Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 417 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 1260, 1558, 1882 |
| AccI | GTMKAC | 1 cut(s) | 233 |
| AccIII | TCCGGA | 1 cut(s) | 1716 |
| AciI | CCGC | 2 cut(s) | 483, 1285 |
| AclWI | GGATC | 8 cut(s) | 449, 462, 716, 785, 798, 1211, 1359, 1708 |
| AcsI | RAATTY | 3 cut(s) | 93, 437, 784 |
| AcuI | CTGAAG | 3 cut(s) | 54, 540, 557 |
| AcvI | CACGTG | 1 cut(s) | 474 |
| AfaI | GTAC | 6 cut(s) | 639, 978, 1074, 1118, 1163, 1202 |
| AfeI | AGCGCT | 2 cut(s) | 374, 1406 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 819, 1260, 1558, 1882 |
| AflIII | ACRYGT | 1 cut(s) | 471 |
| AleI | CACNNNNGTG | 1 cut(s) | 1142 |
| AluBI | AGCT | 6 cut(s) | 1097, 1190, 1505, 1675, 1756, 1831 |
| AluI | AGCT | 6 cut(s) | 1097, 1190, 1505, 1675, 1756, 1831 |
| Alw26I | GTCTC | 4 cut(s) | 494, 892, 1421, 1863 |
| AlwI | GGATC | 8 cut(s) | 449, 462, 716, 785, 798, 1211, 1359, 1708 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 32, 1139, 1508 |
| Ama87I | CYCGRG | 2 cut(s) | 18, 1841 |
| Aor13HI | TCCGGA | 1 cut(s) | 1716 |
| Aor51HI | AGCGCT | 2 cut(s) | 374, 1406 |
| AoxI | GGCC | 5 cut(s) | 52, 72, 402, 515, 1361 |
| ApeKI | GCWGC | 5 cut(s) | 185, 1133, 1330, 1548, 1616 |
| ApoI | RAATTY | 3 cut(s) | 93, 437, 784 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1214, 1246, 1779, 1811 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 177, 1687 |
| AspLEI | GCGC | 4 cut(s) | 87, 375, 1335, 1407 |
| AspS9I | GGNCC | 2 cut(s) | 52, 1795 |
| AsuC2I | CCSGG | 1 cut(s) | 1799 |
| AsuHPI | GGTGA | 3 cut(s) | 296, 350, 1335 |
| AvaI | CYCGRG | 2 cut(s) | 18, 1841 |
| AvaII | GGWCC | 1 cut(s) | 1795 |
| BamHI | GGATCC | 2 cut(s) | 454, 790 |
| BanII | GRGCYC | 1 cut(s) | 126 |
| BbrPI | CACGTG | 1 cut(s) | 474 |
| BbsI | GAAGAC | 1 cut(s) | 1737 |
| BbvI | GCAGC | 5 cut(s) | 172, 1145, 1342, 1560, 1603 |
| BccI | CCATC | 4 cut(s) | 246, 1348, 1560, 1720 |
| BceAI | ACGGC | 2 cut(s) | 1183, 1671 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1595, 1629, 1878, 1912 |
| BciVI | GTATCC | 1 cut(s) | 1056 |
| BcnI | CCSGG | 1 cut(s) | 1799 |
| BcoDI | GTCTC | 4 cut(s) | 494, 892, 1421, 1863 |
| BfaI | CTAG | 3 cut(s) | 102, 1400, 1912 |
| BfmI | CTRYAG | 2 cut(s) | 807, 1140 |
| BfoI | RGCGCY | 3 cut(s) | 376, 1336, 1408 |
| BfuI | GTATCC | 1 cut(s) | 1056 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 23 |
| BglII | AGATCT | 1 cut(s) | 532 |
| BisI | GCNGC | 5 cut(s) | 186, 1134, 1331, 1549, 1617 |
| BlsI | GCNGC | 5 cut(s) | 187, 1135, 1332, 1550, 1618 |
| BmcAI | AGTACT | 1 cut(s) | 1074 |
| Bme1390I | CCNGG | 1 cut(s) | 1799 |
| Bme18I | GGWCC | 1 cut(s) | 1795 |
| BmeT110I | CYCGRG | 2 cut(s) | 18, 1841 |
| BmgT120I | GGNCC | 2 cut(s) | 52, 1795 |
| BmiI | GGNNCC | 4 cut(s) | 123, 456, 792, 1797 |
| BmrFI | CCNGG | 1 cut(s) | 1799 |
| BmsI | GCATC | 8 cut(s) | 55, 72, 371, 667, 772, 856, 1120, 1603 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1278 |
| BpiI | GAAGAC | 1 cut(s) | 1737 |
| BpmI | CTGGAG | 1 cut(s) | 1580 |
| Bpu10I | CCTNAGC | 1 cut(s) | 69 |
| BpuEI | CTTGAG | 3 cut(s) | 233, 278, 1655 |
| BpuMI | CCSGG | 1 cut(s) | 1799 |
| BsaAI | YACGTR | 1 cut(s) | 474 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1029 |
| BsaI | GGTCTC | 2 cut(s) | 494, 1421 |
| BsaJI | CCNNGG | 4 cut(s) | 88, 705, 795, 840 |
| BsaWI | WCCGGW | 1 cut(s) | 1716 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 819, 1260, 1558, 1882 |
| Bse118I | RCCGGY | 2 cut(s) | 485, 1778 |
| Bse1I | ACTGG | 5 cut(s) | 417, 574, 1057, 1563, 1630 |
| Bse3DI | GCAATG | 1 cut(s) | 1739 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1029 |
| BseAI | TCCGGA | 1 cut(s) | 1716 |
| BseDI | CCNNGG | 4 cut(s) | 88, 705, 795, 840 |
| BseGI | GGATG | 5 cut(s) | 219, 924, 1009, 1294, 1634 |
| BseJI | GATNNNNATC | 1 cut(s) | 1029 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 819, 1260, 1558, 1882 |
| BseMI | GCAATG | 1 cut(s) | 1739 |
| BseMII | CTCAG | 4 cut(s) | 914, 1035, 1083, 1140 |
| BseNI | ACTGG | 5 cut(s) | 417, 574, 1057, 1563, 1630 |
| BseXI | GCAGC | 5 cut(s) | 172, 1145, 1342, 1560, 1603 |
| BseYI | CCCAGC | 1 cut(s) | 375 |
| BshFI | GGCC | 5 cut(s) | 54, 74, 404, 517, 1363 |
| BsiHKCI | CYCGRG | 2 cut(s) | 18, 1841 |
| BsiSI | CCGG | 4 cut(s) | 486, 1717, 1779, 1799 |
| BsiWI | CGTACG | 1 cut(s) | 1116 |
| BslFI | GGGAC | 1 cut(s) | 1781 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 819, 1260, 1558, 1882 |
| BsmAI | GTCTC | 4 cut(s) | 494, 892, 1421, 1863 |
| BsmFI | GGGAC | 1 cut(s) | 1781 |
| BsmI | GAATGC | 1 cut(s) | 976 |
| BsnI | GGCC | 5 cut(s) | 54, 74, 404, 517, 1363 |
| Bso31I | GGTCTC | 2 cut(s) | 494, 1421 |
| BsoBI | CYCGRG | 2 cut(s) | 18, 1841 |
| Bsp1286I | GDGCHC | 1 cut(s) | 126 |
| Bsp13I | TCCGGA | 1 cut(s) | 1716 |
| Bsp143I | GATC | 8 cut(s) | 216, 454, 532, 721, 790, 1216, 1351, 1713 |
| BspACI | CCGC | 2 cut(s) | 483, 1285 |
| BspANI | GGCC | 5 cut(s) | 54, 74, 404, 517, 1363 |
| BspCNI | CTCAG | 4 cut(s) | 913, 1034, 1082, 1139 |
| BspEI | TCCGGA | 1 cut(s) | 1716 |
| BspHI | TCATGA | 1 cut(s) | 574 |
| BspLI | GGNNCC | 4 cut(s) | 123, 456, 792, 1797 |
| BspMAI | CTGCAG | 1 cut(s) | 1144 |
| BspPI | GGATC | 8 cut(s) | 449, 462, 716, 785, 798, 1211, 1359, 1708 |
| BspQI | GCTCTTC | 1 cut(s) | 876 |
| BspTNI | GGTCTC | 2 cut(s) | 494, 1421 |
| BsrDI | GCAATG | 1 cut(s) | 1739 |
| BsrFI | RCCGGY | 2 cut(s) | 485, 1778 |
| BsrI | ACTGG | 5 cut(s) | 417, 574, 1057, 1563, 1630 |
| BssAI | RCCGGY | 2 cut(s) | 485, 1778 |
| BssECI | CCNNGG | 4 cut(s) | 88, 705, 795, 840 |
| BssMI | GATC | 8 cut(s) | 216, 454, 532, 721, 790, 1216, 1351, 1713 |
| BssNAI | GTATAC | 1 cut(s) | 234 |
| BssT1I | CCWWGG | 4 cut(s) | 88, 705, 795, 840 |
| Bst1107I | GTATAC | 1 cut(s) | 234 |
| Bst4CI | ACNGT | 6 cut(s) | 231, 308, 808, 966, 1326, 1597 |
| Bst6I | CTCTTC | 4 cut(s) | 242, 393, 497, 876 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1139, 1148 |
| BstBAI | YACGTR | 1 cut(s) | 474 |
| BstC8I | GCNNGC | 6 cut(s) | 515, 995, 1365, 1780, 1873, 1877 |
| BstDEI | CTNAG | 6 cut(s) | 69, 699, 900, 1021, 1069, 1126 |
| BstEII | GGTNACC | 1 cut(s) | 302 |
| BstF5I | GGATG | 5 cut(s) | 219, 924, 1009, 1294, 1634 |
| BstH2I | RGCGCY | 3 cut(s) | 376, 1336, 1408 |
| BstHHI | GCGC | 4 cut(s) | 87, 375, 1335, 1407 |
| BstKTI | GATC | 8 cut(s) | 219, 457, 535, 724, 793, 1219, 1354, 1716 |
| BstMAI | GTCTC | 4 cut(s) | 494, 892, 1421, 1863 |
| BstMBI | GATC | 8 cut(s) | 216, 454, 532, 721, 790, 1216, 1351, 1713 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 23, 182, 769, 1139, 1148 |
| BstNSI | RCATGY | 1 cut(s) | 416 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1278 |
| BstPI | GGTNACC | 1 cut(s) | 302 |
| BstSCI | CCNGG | 1 cut(s) | 1797 |
| BstSFI | CTRYAG | 2 cut(s) | 807, 1140 |
| BstV1I | GCAGC | 5 cut(s) | 172, 1145, 1342, 1560, 1603 |
| BstV2I | GAAGAC | 1 cut(s) | 1737 |
| BstX2I | RGATCY | 4 cut(s) | 454, 532, 790, 1713 |
| BstYI | RGATCY | 4 cut(s) | 454, 532, 790, 1713 |
| BstZ17I | GTATAC | 1 cut(s) | 234 |
| BsuI | GTATCC | 1 cut(s) | 1056 |
| BsuRI | GGCC | 5 cut(s) | 54, 74, 404, 517, 1363 |
| BtgZI | GCGATG | 2 cut(s) | 1188, 1781 |
| BtsCI | GGATG | 5 cut(s) | 219, 924, 1009, 1294, 1634 |
| BtsI | GCAGTG | 4 cut(s) | 33, 163, 1137, 1149 |
| BtsIMutI | CAGTG | 5 cut(s) | 33, 163, 1064, 1137, 1149 |
| Cac8I | GCNNGC | 6 cut(s) | 515, 995, 1365, 1780, 1873, 1877 |
| CaiI | CAGNNNCTG | 3 cut(s) | 32, 1139, 1508 |
| CciI | TCATGA | 1 cut(s) | 574 |
| CfoI | GCGC | 4 cut(s) | 87, 375, 1335, 1407 |
| Cfr10I | RCCGGY | 2 cut(s) | 485, 1778 |
| Cfr13I | GGNCC | 2 cut(s) | 52, 1795 |
| Csp6I | GTAC | 6 cut(s) | 638, 977, 1073, 1117, 1162, 1201 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 278, 313 |
| CviAII | CATG | 5 cut(s) | 127, 182, 413, 575, 683 |
| CviQI | GTAC | 6 cut(s) | 638, 977, 1073, 1117, 1162, 1201 |
| DdeI | CTNAG | 6 cut(s) | 69, 699, 900, 1021, 1069, 1126 |
| DpnI | GATC | 8 cut(s) | 218, 456, 534, 723, 792, 1218, 1353, 1715 |
| DpnII | GATC | 8 cut(s) | 216, 454, 532, 721, 790, 1216, 1351, 1713 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 417 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 417 |
| Eam1104I | CTCTTC | 4 cut(s) | 242, 393, 497, 876 |
| EarI | CTCTTC | 4 cut(s) | 242, 393, 497, 876 |
| EciI | GGCGGA | 1 cut(s) | 472 |
| Eco130I | CCWWGG | 4 cut(s) | 88, 705, 795, 840 |
| Eco24I | GRGCYC | 1 cut(s) | 126 |
| Eco31I | GGTCTC | 2 cut(s) | 494, 1421 |
| Eco32I | GATATC | 1 cut(s) | 1896 |
| Eco47I | GGWCC | 1 cut(s) | 1795 |
| Eco47III | AGCGCT | 2 cut(s) | 374, 1406 |
| Eco57I | CTGAAG | 3 cut(s) | 54, 540, 557 |
| Eco72I | CACGTG | 1 cut(s) | 474 |
| Eco88I | CYCGRG | 2 cut(s) | 18, 1841 |
| Eco91I | GGTNACC | 1 cut(s) | 302 |
| EcoO109I | RGGNCCY | 2 cut(s) | 52, 1795 |
| EcoO65I | GGTNACC | 1 cut(s) | 302 |
| EcoRV | GATATC | 1 cut(s) | 1896 |
| EcoT14I | CCWWGG | 4 cut(s) | 88, 705, 795, 840 |
| EcoT38I | GRGCYC | 1 cut(s) | 126 |
| ErhI | CCWWGG | 4 cut(s) | 88, 705, 795, 840 |
| FaeI | CATG | 5 cut(s) | 130, 185, 416, 578, 686 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 41, 73, 162, 194, 1187, 1219, 1689, 1721 |
| FaqI | GGGAC | 1 cut(s) | 1781 |
| FatI | CATG | 5 cut(s) | 126, 181, 412, 574, 682 |
| FauNDI | CATATG | 1 cut(s) | 1881 |
| FblI | GTMKAC | 1 cut(s) | 233 |
| Fnu4HI | GCNGC | 5 cut(s) | 186, 1134, 1331, 1549, 1617 |
| FokI | GGATG | 5 cut(s) | 206, 911, 1016, 1281, 1641 |
| FriOI | GRGCYC | 1 cut(s) | 126 |
| Fsp4HI | GCNGC | 5 cut(s) | 186, 1134, 1331, 1549, 1617 |
| FspBI | CTAG | 3 cut(s) | 102, 1400, 1912 |
| GlaI | GCGC | 4 cut(s) | 86, 374, 1334, 1406 |
| GluI | GCNGC | 5 cut(s) | 186, 1134, 1331, 1549, 1617 |
| GsaI | CCCAGC | 1 cut(s) | 379 |
| GsuI | CTGGAG | 1 cut(s) | 1580 |
| HaeII | RGCGCY | 3 cut(s) | 376, 1336, 1408 |
| HaeIII | GGCC | 5 cut(s) | 54, 74, 404, 517, 1363 |
| HapII | CCGG | 4 cut(s) | 486, 1717, 1779, 1799 |
| HhaI | GCGC | 4 cut(s) | 87, 375, 1335, 1407 |
| Hin1II | CATG | 5 cut(s) | 130, 185, 416, 578, 686 |
| Hin6I | GCGC | 4 cut(s) | 85, 373, 1333, 1405 |
| HinP1I | GCGC | 4 cut(s) | 85, 373, 1333, 1405 |
| HindIII | AAGCTT | 2 cut(s) | 1673, 1829 |
| HinfI | GANTC | 6 cut(s) | 670, 1229, 1438, 1448, 1807, 1835 |
| HpaII | CCGG | 4 cut(s) | 486, 1717, 1779, 1799 |
| HphI | GGTGA | 3 cut(s) | 296, 350, 1335 |
| Hpy166II | GTNNAC | 3 cut(s) | 234, 1472, 1648 |
| Hpy188I | TCNGA | 8 cut(s) | 367, 537, 768, 907, 1024, 1129, 1234, 1812 |
| Hpy8I | GTNNAC | 3 cut(s) | 234, 1472, 1648 |
| HpyAV | CCTTC | 9 cut(s) | 43, 188, 532, 749, 812, 1346, 1401, 1756, 1840 |
| HpyCH4III | ACNGT | 6 cut(s) | 231, 308, 808, 966, 1326, 1597 |
| HpyCH4IV | ACGT | 3 cut(s) | 7, 473, 636 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 23, 182, 769, 1139, 1148 |
| HpyF3I | CTNAG | 6 cut(s) | 69, 699, 900, 1021, 1069, 1126 |
| HpySE526I | ACGT | 3 cut(s) | 7, 473, 636 |
| Hsp92II | CATG | 5 cut(s) | 130, 185, 416, 578, 686 |
| HspAI | GCGC | 4 cut(s) | 85, 373, 1333, 1405 |
| Kpn2I | TCCGGA | 1 cut(s) | 1716 |
| KroI | GCCGGC | 1 cut(s) | 1778 |
| KroNI | GCCGGC | 1 cut(s) | 1780 |
| Kzo9I | GATC | 8 cut(s) | 216, 454, 532, 721, 790, 1216, 1351, 1713 |
| LguI | GCTCTTC | 1 cut(s) | 876 |
| LmnI | GCTCC | 2 cut(s) | 121, 359 |
| Lsp1109I | GCAGC | 5 cut(s) | 172, 1145, 1342, 1560, 1603 |
| LweI | GCATC | 8 cut(s) | 55, 72, 371, 667, 772, 856, 1120, 1603 |
| MaeI | CTAG | 3 cut(s) | 102, 1400, 1912 |
| MaeII | ACGT | 3 cut(s) | 7, 473, 636 |
| MaeIII | GTNAC | 3 cut(s) | 28, 302, 1312 |
| MalI | GATC | 8 cut(s) | 218, 456, 534, 723, 792, 1218, 1353, 1715 |
| MboI | GATC | 8 cut(s) | 216, 454, 532, 721, 790, 1216, 1351, 1713 |
| MfeI | CAATTG | 1 cut(s) | 443 |
| MflI | RGATCY | 4 cut(s) | 454, 532, 790, 1713 |
| MhlI | GDGCHC | 1 cut(s) | 126 |
| MluCI | AATT | 8 cut(s) | 93, 352, 437, 443, 784, 800, 1371, 1579 |
| MlyI | GAGTC | 1 cut(s) | 1238 |
| MmeI | TCCRAC | 1 cut(s) | 1089 |
| MroI | TCCGGA | 1 cut(s) | 1716 |
| MroNI | GCCGGC | 1 cut(s) | 1778 |
| MroXI | GAANNNNTTC | 2 cut(s) | 177, 1687 |
| MseI | TTAA | 2 cut(s) | 41, 150 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1142 |
| MspI | CCGG | 4 cut(s) | 486, 1717, 1779, 1799 |
| MspR9I | CCNGG | 1 cut(s) | 1799 |
| MunI | CAATTG | 1 cut(s) | 443 |
| Mva1269I | GAATGC | 1 cut(s) | 976 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 23, 182, 769, 1139, 1148 |
| NaeI | GCCGGC | 1 cut(s) | 1780 |
| NciI | CCSGG | 1 cut(s) | 1799 |
| NdeI | CATATG | 1 cut(s) | 1881 |
| NdeII | GATC | 8 cut(s) | 216, 454, 532, 721, 790, 1216, 1351, 1713 |
| NgoMIV | GCCGGC | 1 cut(s) | 1778 |
| NlaIII | CATG | 5 cut(s) | 130, 185, 416, 578, 686 |
| NlaIV | GGNNCC | 4 cut(s) | 123, 456, 792, 1797 |
| NmuCI | GTSAC | 3 cut(s) | 28, 302, 1312 |
| NspI | RCATGY | 1 cut(s) | 416 |
| OliI | CACNNNNGTG | 1 cut(s) | 1142 |
| PaeR7I | CTCGAG | 1 cut(s) | 1841 |
| PagI | TCATGA | 1 cut(s) | 574 |
| PciSI | GCTCTTC | 1 cut(s) | 876 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1112, 1495 |
| PctI | GAATGC | 1 cut(s) | 976 |
| PdiI | GCCGGC | 1 cut(s) | 1780 |
| PdmI | GAANNNNTTC | 2 cut(s) | 177, 1687 |
| PfeI | GAWTC | 5 cut(s) | 670, 1438, 1448, 1807, 1835 |
| Pfl23II | CGTACG | 1 cut(s) | 1116 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 1260, 1558, 1882 |
| PfoI | TCCNGGA | 1 cut(s) | 1797 |
| PkrI | GCNGC | 5 cut(s) | 187, 1135, 1332, 1550, 1618 |
| PleI | GAGTC | 1 cut(s) | 1237 |
| PmaCI | CACGTG | 1 cut(s) | 474 |
| PmlI | CACGTG | 1 cut(s) | 474 |
| PpsI | GAGTC | 1 cut(s) | 1237 |
| Ppu21I | YACGTR | 1 cut(s) | 474 |
| PpuMI | RGGWCCY | 1 cut(s) | 1795 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1278 |
| Psp5II | RGGWCCY | 1 cut(s) | 1795 |
| PspCI | CACGTG | 1 cut(s) | 474 |
| PspEI | GGTNACC | 1 cut(s) | 302 |
| PspFI | CCCAGC | 1 cut(s) | 375 |
| PspLI | CGTACG | 1 cut(s) | 1116 |
| PspN4I | GGNNCC | 4 cut(s) | 123, 456, 792, 1797 |
| PspPI | GGNCC | 2 cut(s) | 52, 1795 |
| PspPPI | RGGWCCY | 1 cut(s) | 1795 |
| PstI | CTGCAG | 1 cut(s) | 1144 |
| PstNI | CAGNNNCTG | 3 cut(s) | 32, 1139, 1508 |
| PsuI | RGATCY | 4 cut(s) | 454, 532, 790, 1713 |
| RsaI | GTAC | 6 cut(s) | 639, 978, 1074, 1118, 1163, 1202 |
| RsaNI | GTAC | 6 cut(s) | 638, 977, 1073, 1117, 1162, 1201 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1142 |
| SapI | GCTCTTC | 1 cut(s) | 876 |
| SaqAI | TTAA | 2 cut(s) | 41, 150 |
| SatI | GCNGC | 5 cut(s) | 186, 1134, 1331, 1549, 1617 |
| Sau3AI | GATC | 8 cut(s) | 216, 454, 532, 721, 790, 1216, 1351, 1713 |
| Sau96I | GGNCC | 2 cut(s) | 52, 1795 |
| ScaI | AGTACT | 1 cut(s) | 1074 |
| SchI | GAGTC | 1 cut(s) | 1238 |
| ScrFI | CCNGG | 1 cut(s) | 1799 |
| SduI | GDGCHC | 1 cut(s) | 126 |
| SfaNI | GCATC | 8 cut(s) | 55, 72, 371, 667, 772, 856, 1120, 1603 |
| SfcI | CTRYAG | 2 cut(s) | 807, 1140 |
| Sfr274I | CTCGAG | 1 cut(s) | 1841 |
| SinI | GGWCC | 1 cut(s) | 1795 |
| SlaI | CTCGAG | 1 cut(s) | 1841 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1142 |
| SmlI | CTYRAG | 4 cut(s) | 212, 257, 1670, 1841 |
| SmoI | CTYRAG | 4 cut(s) | 212, 257, 1670, 1841 |
| Sse9I | AATT | 8 cut(s) | 93, 352, 437, 443, 784, 800, 1371, 1579 |
| SsiI | CCGC | 2 cut(s) | 483, 1285 |
| SspMI | CTAG | 3 cut(s) | 102, 1400, 1912 |
| StyD4I | CCNGG | 1 cut(s) | 1797 |
| StyI | CCWWGG | 4 cut(s) | 88, 705, 795, 840 |
| TaaI | ACNGT | 6 cut(s) | 231, 308, 808, 966, 1326, 1597 |
| TaiI | ACGT | 3 cut(s) | 10, 476, 639 |
| TaqI | TCGA | 5 cut(s) | 1519, 1760, 1842, 1863, 1898 |
| TaqII | GACCGA | 1 cut(s) | 1445 |
| TasI | AATT | 8 cut(s) | 93, 352, 437, 443, 784, 800, 1371, 1579 |
| TatI | WGTACW | 1 cut(s) | 1072 |
| TfiI | GAWTC | 5 cut(s) | 670, 1438, 1448, 1807, 1835 |
| Tru1I | TTAA | 2 cut(s) | 41, 150 |
| Tru9I | TTAA | 2 cut(s) | 41, 150 |
| TscAI | CASTG | 5 cut(s) | 33, 163, 1064, 1144, 1149 |
| TseFI | GTSAC | 3 cut(s) | 28, 302, 1312 |
| TseI | GCWGC | 5 cut(s) | 185, 1133, 1330, 1548, 1616 |
| Tsp45I | GTSAC | 3 cut(s) | 28, 302, 1312 |
| TspDTI | ATGAA | 7 cut(s) | 170, 408, 1283, 1431, 1451, 1680, 1784 |
| TspGWI | ACGGA | 2 cut(s) | 1287, 1457 |
| TspRI | CASTG | 5 cut(s) | 33, 163, 1064, 1144, 1149 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 1260, 1558, 1882 |
| VpaK11BI | GGWCC | 1 cut(s) | 1795 |
| XapI | RAATTY | 3 cut(s) | 93, 437, 784 |
| XbaI | TCTAGA | 2 cut(s) | 101, 1399 |
| XceI | RCATGY | 1 cut(s) | 416 |
| XhoI | CTCGAG | 1 cut(s) | 1841 |
| XmiI | GTMKAC | 1 cut(s) | 233 |
| XmnI | GAANNNNTTC | 2 cut(s) | 177, 1687 |
| XspI | CTAG | 3 cut(s) | 102, 1400, 1912 |
| ZrmI | AGTACT | 1 cut(s) | 1074 |