Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 744, 1257, 2502 |
| AccB1I | GGYRCC | 1 cut(s) | 629 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1106, 2856 |
| AccI | GTMKAC | 2 cut(s) | 1484, 1712 |
| AccII | CGCG | 1 cut(s) | 1264 |
| AccIII | TCCGGA | 1 cut(s) | 1000 |
| AciI | CCGC | 4 cut(s) | 380, 457, 460, 2770 |
| AclWI | GGATC | 4 cut(s) | 1091, 1136, 2003, 2501 |
| AcoI | YGGCCR | 3 cut(s) | 1781, 2771, 2858 |
| AcsI | RAATTY | 4 cut(s) | 1103, 1235, 1676, 2924 |
| AcuI | CTGAAG | 3 cut(s) | 113, 878, 1299 |
| AfaI | GTAC | 4 cut(s) | 942, 1909, 2364, 2453 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 555, 735, 1055, 1106, 1564, 2106, 2856 |
| AgsI | TTSAA | 8 cut(s) | 189, 260, 842, 1832, 2042, 2064, 2477, 2913 |
| AjnI | CCWGG | 4 cut(s) | 490, 563, 865, 2773 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 957, 989 |
| AleI | CACNNNNGTG | 1 cut(s) | 398 |
| Alw21I | GWGCWC | 1 cut(s) | 87 |
| Alw26I | GTCTC | 4 cut(s) | 258, 659, 1260, 2800 |
| AlwI | GGATC | 4 cut(s) | 1091, 1136, 2003, 2501 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1667 |
| Ama87I | CYCGRG | 1 cut(s) | 1534 |
| Aor13HI | TCCGGA | 1 cut(s) | 1000 |
| AoxI | GGCC | 6 cut(s) | 412, 892, 1781, 2557, 2771, 2858 |
| ApeKI | GCWGC | 7 cut(s) | 428, 640, 643, 1667, 1924, 2423, 2627 |
| ApoI | RAATTY | 4 cut(s) | 1103, 1235, 1676, 2924 |
| AseI | ATTAAT | 1 cut(s) | 2319 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2120 |
| AspLEI | GCGC | 5 cut(s) | 27, 1138, 1170, 1264, 1414 |
| AspS9I | GGNCC | 3 cut(s) | 217, 499, 1720 |
| AsuC2I | CCSGG | 2 cut(s) | 556, 1133 |
| AsuHPI | GGTGA | 6 cut(s) | 989, 1285, 1547, 1910, 2806, 2878 |
| AsuII | TTCGAA | 1 cut(s) | 1155 |
| AvaI | CYCGRG | 1 cut(s) | 1534 |
| AvaII | GGWCC | 3 cut(s) | 217, 499, 1720 |
| AxyI | CCTNAGG | 2 cut(s) | 1599, 2561 |
| BalI | TGGCCA | 1 cut(s) | 1783 |
| BanI | GGYRCC | 1 cut(s) | 629 |
| BanII | GRGCYC | 2 cut(s) | 87, 1371 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 924, 956 |
| BbsI | GAAGAC | 3 cut(s) | 1803, 1812, 2606 |
| Bbv12I | GWGCWC | 1 cut(s) | 87 |
| BbvI | GCAGC | 7 cut(s) | 415, 652, 655, 1654, 1911, 2435, 2639 |
| BccI | CCATC | 8 cut(s) | 1199, 1497, 1620, 1898, 2641, 2668, 2698, 2895 |
| BceAI | ACGGC | 4 cut(s) | 101, 636, 2783, 2845 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2223, 2257 |
| BciT130I | CCWGG | 4 cut(s) | 492, 565, 867, 2775 |
| BciVI | GTATCC | 3 cut(s) | 387, 2346, 2878 |
| BclI | TGATCA | 1 cut(s) | 1489 |
| BcnI | CCSGG | 2 cut(s) | 556, 1133 |
| BcoDI | GTCTC | 4 cut(s) | 258, 659, 1260, 2800 |
| BfaI | CTAG | 5 cut(s) | 410, 1347, 1463, 1656, 2747 |
| BfmI | CTRYAG | 2 cut(s) | 147, 760 |
| BfoI | RGCGCY | 1 cut(s) | 1171 |
| BfuI | GTATCC | 3 cut(s) | 387, 2346, 2878 |
| BisI | GCNGC | 9 cut(s) | 429, 458, 641, 644, 1668, 1925, 2424, 2628, 2771 |
| BlsI | GCNGC | 9 cut(s) | 430, 459, 642, 645, 1669, 1926, 2425, 2629, 2772 |
| BmcAI | AGTACT | 1 cut(s) | 2364 |
| Bme1390I | CCNGG | 6 cut(s) | 492, 556, 565, 867, 1133, 2775 |
| Bme18I | GGWCC | 3 cut(s) | 217, 499, 1720 |
| BmeT110I | CYCGRG | 1 cut(s) | 1534 |
| BmgT120I | GGNCC | 3 cut(s) | 217, 499, 1720 |
| BmiI | GGNNCC | 5 cut(s) | 219, 631, 864, 1368, 1722 |
| BmrFI | CCNGG | 6 cut(s) | 492, 556, 565, 867, 1133, 2775 |
| BmrI | ACTGGG | 2 cut(s) | 793, 1845 |
| BmsI | GCATC | 9 cut(s) | 331, 1006, 1030, 1072, 1236, 1539, 1954, 2143, 2410 |
| BmuI | ACTGGG | 2 cut(s) | 793, 1845 |
| BpiI | GAAGAC | 3 cut(s) | 1803, 1812, 2606 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 692, 724, 812, 844 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2427 |
| Bpu14I | TTCGAA | 1 cut(s) | 1155 |
| BpuEI | CTTGAG | 3 cut(s) | 1199, 1271, 1529 |
| BpuMI | CCSGG | 2 cut(s) | 556, 1133 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1331 |
| BsaJI | CCNNGG | 4 cut(s) | 38, 213, 1131, 2664 |
| BsaWI | WCCGGW | 1 cut(s) | 1000 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 555, 735, 1055, 1106, 1564, 2106, 2856 |
| Bse21I | CCTNAGG | 2 cut(s) | 1599, 2561 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1331 |
| BseAI | TCCGGA | 1 cut(s) | 1000 |
| BseBI | CCWGG | 4 cut(s) | 492, 565, 867, 2775 |
| BseDI | CCNNGG | 4 cut(s) | 38, 213, 1131, 2664 |
| BseJI | GATNNNNATC | 1 cut(s) | 1331 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 555, 735, 1055, 1106, 1564, 2106, 2856 |
| BseMII | CTCAG | 8 cut(s) | 533, 1305, 1397, 1590, 1730, 2373, 2552, 2804 |
| BseRI | GAGGAG | 2 cut(s) | 1451, 2342 |
| BseXI | GCAGC | 7 cut(s) | 415, 652, 655, 1654, 1911, 2435, 2639 |
| BseYI | CCCAGC | 1 cut(s) | 506 |
| BsgI | GTGCAG | 3 cut(s) | 1931, 2169, 2169 |
| Bsh1236I | CGCG | 1 cut(s) | 1264 |
| BshFI | GGCC | 6 cut(s) | 414, 894, 1783, 2559, 2773, 2860 |
| BshNI | GGYRCC | 1 cut(s) | 629 |
| BsiHKAI | GWGCWC | 1 cut(s) | 87 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1534 |
| BsiSI | CCGG | 4 cut(s) | 555, 1001, 1133, 1565 |
| BslFI | GGGAC | 2 cut(s) | 452, 2303 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 555, 735, 1055, 1106, 1564, 2106, 2856 |
| BsmAI | GTCTC | 4 cut(s) | 258, 659, 1260, 2800 |
| BsmBI | CGTCTC | 1 cut(s) | 659 |
| BsmFI | GGGAC | 2 cut(s) | 452, 2303 |
| BsmI | GAATGC | 3 cut(s) | 1808, 2263, 2737 |
| BsnI | GGCC | 6 cut(s) | 414, 894, 1783, 2559, 2773, 2860 |
| BsoBI | CYCGRG | 1 cut(s) | 1534 |
| Bsp119I | TTCGAA | 1 cut(s) | 1155 |
| Bsp1286I | GDGCHC | 2 cut(s) | 87, 1371 |
| Bsp13I | TCCGGA | 1 cut(s) | 1000 |
| Bsp143I | GATC | 8 cut(s) | 3, 571, 1096, 1141, 1467, 1489, 2008, 2506 |
| Bsp19I | CCATGG | 2 cut(s) | 38, 2664 |
| BspACI | CCGC | 4 cut(s) | 380, 457, 460, 2770 |
| BspANI | GGCC | 6 cut(s) | 414, 894, 1783, 2559, 2773, 2860 |
| BspCNI | CTCAG | 8 cut(s) | 532, 1306, 1396, 1591, 1729, 2372, 2553, 2803 |
| BspEI | TCCGGA | 1 cut(s) | 1000 |
| BspFNI | CGCG | 1 cut(s) | 1264 |
| BspLI | GGNNCC | 5 cut(s) | 219, 631, 864, 1368, 1722 |
| BspPI | GGATC | 4 cut(s) | 1091, 1136, 2003, 2501 |
| BspQI | GCTCTTC | 1 cut(s) | 2163 |
| BspT104I | TTCGAA | 1 cut(s) | 1155 |
| BspT107I | GGYRCC | 1 cut(s) | 629 |
| BssECI | CCNNGG | 4 cut(s) | 38, 213, 1131, 2664 |
| BssMI | GATC | 8 cut(s) | 3, 571, 1096, 1141, 1467, 1489, 2008, 2506 |
| BssNAI | GTATAC | 1 cut(s) | 1485 |
| BssT1I | CCWWGG | 3 cut(s) | 38, 213, 2664 |
| Bst1107I | GTATAC | 1 cut(s) | 1485 |
| Bst2UI | CCWGG | 4 cut(s) | 492, 565, 867, 2775 |
| Bst4CI | ACNGT | 8 cut(s) | 155, 249, 626, 1559, 1614, 2190, 2294, 2389 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1574, 1667, 2522 |
| BstBI | TTCGAA | 1 cut(s) | 1155 |
| BstC8I | GCNNGC | 4 cut(s) | 455, 1166, 1781, 1867 |
| BstDEI | CTNAG | 9 cut(s) | 519, 1314, 1383, 1599, 1716, 2359, 2427, 2561, 2790 |
| BstDSI | CCRYGG | 2 cut(s) | 38, 2664 |
| BstEII | GGTNACC | 1 cut(s) | 1553 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 733 |
| BstFNI | CGCG | 1 cut(s) | 1264 |
| BstH2I | RGCGCY | 1 cut(s) | 1171 |
| BstHHI | GCGC | 5 cut(s) | 27, 1138, 1170, 1264, 1414 |
| BstKTI | GATC | 8 cut(s) | 6, 574, 1099, 1144, 1470, 1492, 2011, 2509 |
| BstMAI | GTCTC | 4 cut(s) | 258, 659, 1260, 2800 |
| BstMBI | GATC | 8 cut(s) | 3, 571, 1096, 1141, 1467, 1489, 2008, 2506 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 113, 425, 512, 1418, 1574, 1667, 2429, 2522 |
| BstNI | CCWGG | 4 cut(s) | 492, 565, 867, 2775 |
| BstNSI | RCATGY | 1 cut(s) | 1040 |
| BstPI | GGTNACC | 1 cut(s) | 1553 |
| BstSCI | CCNGG | 6 cut(s) | 490, 554, 563, 865, 1131, 2773 |
| BstSFI | CTRYAG | 2 cut(s) | 147, 760 |
| BstUI | CGCG | 1 cut(s) | 1264 |
| BstV1I | GCAGC | 7 cut(s) | 415, 652, 655, 1654, 1911, 2435, 2639 |
| BstV2I | GAAGAC | 3 cut(s) | 1803, 1812, 2606 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1226, 2901 |
| BstZ17I | GTATAC | 1 cut(s) | 1485 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1599, 2561 |
| BsuI | GTATCC | 3 cut(s) | 387, 2346, 2878 |
| BsuRI | GGCC | 6 cut(s) | 414, 894, 1783, 2559, 2773, 2860 |
| BtgI | CCRYGG | 2 cut(s) | 38, 2664 |
| BtsI | GCAGTG | 3 cut(s) | 423, 1668, 2181 |
| Cac8I | GCNNGC | 4 cut(s) | 455, 1166, 1781, 1867 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1667 |
| CfoI | GCGC | 5 cut(s) | 27, 1138, 1170, 1264, 1414 |
| Cfr13I | GGNCC | 3 cut(s) | 217, 499, 1720 |
| Csp6I | GTAC | 4 cut(s) | 941, 1908, 2363, 2452 |
| CspCI | CAANNNNNGTGG | 8 cut(s) | 270, 305, 699, 734, 1529, 1564, 2125, 2160 |
| CviQI | GTAC | 4 cut(s) | 941, 1908, 2363, 2452 |
| DdeI | CTNAG | 9 cut(s) | 519, 1314, 1383, 1599, 1716, 2359, 2427, 2561, 2790 |
| DpnI | GATC | 8 cut(s) | 5, 573, 1098, 1143, 1469, 1491, 2010, 2508 |
| DpnII | GATC | 8 cut(s) | 3, 571, 1096, 1141, 1467, 1489, 2008, 2506 |
| EaeI | YGGCCR | 3 cut(s) | 1781, 2771, 2858 |
| EciI | GGCGGA | 2 cut(s) | 369, 475 |
| Ecl136II | GAGCTC | 1 cut(s) | 85 |
| Eco130I | CCWWGG | 3 cut(s) | 38, 213, 2664 |
| Eco24I | GRGCYC | 2 cut(s) | 87, 1371 |
| Eco32I | GATATC | 2 cut(s) | 2231, 2817 |
| Eco47I | GGWCC | 3 cut(s) | 217, 499, 1720 |
| Eco53kI | GAGCTC | 1 cut(s) | 85 |
| Eco57I | CTGAAG | 3 cut(s) | 113, 878, 1299 |
| Eco81I | CCTNAGG | 2 cut(s) | 1599, 2561 |
| Eco88I | CYCGRG | 1 cut(s) | 1534 |
| Eco91I | GGTNACC | 1 cut(s) | 1553 |
| EcoICRI | GAGCTC | 1 cut(s) | 85 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 733 |
| EcoO109I | RGGNCCY | 2 cut(s) | 217, 1720 |
| EcoO65I | GGTNACC | 1 cut(s) | 1553 |
| EcoRII | CCWGG | 4 cut(s) | 490, 563, 865, 2773 |
| EcoRV | GATATC | 2 cut(s) | 2231, 2817 |
| EcoT14I | CCWWGG | 3 cut(s) | 38, 213, 2664 |
| EcoT38I | GRGCYC | 2 cut(s) | 87, 1371 |
| ErhI | CCWWGG | 3 cut(s) | 38, 213, 2664 |
| Esp3I | CGTCTC | 1 cut(s) | 659 |
| FaqI | GGGAC | 2 cut(s) | 452, 2303 |
| FbaI | TGATCA | 1 cut(s) | 1489 |
| FblI | GTMKAC | 2 cut(s) | 1484, 1712 |
| Fnu4HI | GCNGC | 9 cut(s) | 429, 458, 641, 644, 1668, 1925, 2424, 2628, 2771 |
| FriOI | GRGCYC | 2 cut(s) | 87, 1371 |
| Fsp4HI | GCNGC | 9 cut(s) | 429, 458, 641, 644, 1668, 1925, 2424, 2628, 2771 |
| FspBI | CTAG | 5 cut(s) | 410, 1347, 1463, 1656, 2747 |
| GlaI | GCGC | 5 cut(s) | 26, 1137, 1169, 1263, 1413 |
| GluI | GCNGC | 9 cut(s) | 429, 458, 641, 644, 1668, 1925, 2424, 2628, 2771 |
| GsaI | CCCAGC | 1 cut(s) | 510 |
| HaeII | RGCGCY | 1 cut(s) | 1171 |
| HaeIII | GGCC | 6 cut(s) | 414, 894, 1783, 2559, 2773, 2860 |
| HapII | CCGG | 4 cut(s) | 555, 1001, 1133, 1565 |
| HhaI | GCGC | 5 cut(s) | 27, 1138, 1170, 1264, 1414 |
| Hin6I | GCGC | 5 cut(s) | 25, 1136, 1168, 1262, 1412 |
| HinP1I | GCGC | 5 cut(s) | 25, 1136, 1168, 1262, 1412 |
| HincII | GTYRAC | 2 cut(s) | 781, 2765 |
| HindII | GTYRAC | 2 cut(s) | 781, 2765 |
| HindIII | AAGCTT | 3 cut(s) | 125, 1088, 2057 |
| HinfI | GANTC | 6 cut(s) | 226, 827, 1065, 1709, 1957, 2402 |
| HpaII | CCGG | 4 cut(s) | 555, 1001, 1133, 1565 |
| HphI | GGTGA | 6 cut(s) | 989, 1285, 1547, 1910, 2806, 2878 |
| Hpy166II | GTNNAC | 7 cut(s) | 781, 826, 1485, 1713, 2048, 2744, 2765 |
| Hpy188I | TCNGA | 6 cut(s) | 122, 387, 550, 814, 2194, 2419 |
| Hpy8I | GTNNAC | 7 cut(s) | 781, 826, 1485, 1713, 2048, 2744, 2765 |
| HpyCH4III | ACNGT | 8 cut(s) | 155, 249, 626, 1559, 1614, 2190, 2294, 2389 |
| HpyCH4IV | ACGT | 3 cut(s) | 851, 1252, 2611 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 113, 425, 512, 1418, 1574, 1667, 2429, 2522 |
| HpyF3I | CTNAG | 9 cut(s) | 519, 1314, 1383, 1599, 1716, 2359, 2427, 2561, 2790 |
| HpySE526I | ACGT | 3 cut(s) | 851, 1252, 2611 |
| HspAI | GCGC | 5 cut(s) | 25, 1136, 1168, 1262, 1412 |
| Kpn2I | TCCGGA | 1 cut(s) | 1000 |
| Ksp22I | TGATCA | 1 cut(s) | 1489 |
| Kzo9I | GATC | 8 cut(s) | 3, 571, 1096, 1141, 1467, 1489, 2008, 2506 |
| LguI | GCTCTTC | 1 cut(s) | 2163 |
| LmnI | GCTCC | 5 cut(s) | 356, 797, 1366, 2329, 2896 |
| Lsp1109I | GCAGC | 7 cut(s) | 415, 652, 655, 1654, 1911, 2435, 2639 |
| LweI | GCATC | 9 cut(s) | 331, 1006, 1030, 1072, 1236, 1539, 1954, 2143, 2410 |
| MaeI | CTAG | 5 cut(s) | 410, 1347, 1463, 1656, 2747 |
| MaeII | ACGT | 3 cut(s) | 851, 1252, 2611 |
| MaeIII | GTNAC | 6 cut(s) | 391, 852, 1273, 1448, 1520, 1553 |
| MalI | GATC | 8 cut(s) | 5, 573, 1098, 1143, 1469, 1491, 2010, 2508 |
| MboI | GATC | 8 cut(s) | 3, 571, 1096, 1141, 1467, 1489, 2008, 2506 |
| MfeI | CAATTG | 2 cut(s) | 2376, 2810 |
| MhlI | GDGCHC | 2 cut(s) | 87, 1371 |
| MlsI | TGGCCA | 1 cut(s) | 1783 |
| MluNI | TGGCCA | 1 cut(s) | 1783 |
| MlyI | GAGTC | 3 cut(s) | 821, 1718, 2411 |
| MmeI | TCCRAC | 4 cut(s) | 219, 1596, 2534, 2918 |
| Mox20I | TGGCCA | 1 cut(s) | 1783 |
| MroI | TCCGGA | 1 cut(s) | 1000 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2120 |
| MscI | TGGCCA | 1 cut(s) | 1783 |
| MseI | TTAA | 6 cut(s) | 1320, 1703, 1803, 2319, 2846, 2944 |
| MslI | CAYNNNNRTG | 6 cut(s) | 398, 1002, 1026, 1430, 1874, 2622 |
| Msp20I | TGGCCA | 1 cut(s) | 1783 |
| MspI | CCGG | 4 cut(s) | 555, 1001, 1133, 1565 |
| MspR9I | CCNGG | 6 cut(s) | 492, 556, 565, 867, 1133, 2775 |
| MunI | CAATTG | 2 cut(s) | 2376, 2810 |
| Mva1269I | GAATGC | 3 cut(s) | 1808, 2263, 2737 |
| MvaI | CCWGG | 4 cut(s) | 492, 565, 867, 2775 |
| MvnI | CGCG | 1 cut(s) | 1264 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 113, 425, 512, 1418, 1574, 1667, 2429, 2522 |
| NciI | CCSGG | 2 cut(s) | 556, 1133 |
| NcoI | CCATGG | 2 cut(s) | 38, 2664 |
| NdeII | GATC | 8 cut(s) | 3, 571, 1096, 1141, 1467, 1489, 2008, 2506 |
| NlaIV | GGNNCC | 5 cut(s) | 219, 631, 864, 1368, 1722 |
| NmuCI | GTSAC | 4 cut(s) | 391, 1273, 1520, 1553 |
| NspI | RCATGY | 1 cut(s) | 1040 |
| NspV | TTCGAA | 1 cut(s) | 1155 |
| OliI | CACNNNNGTG | 1 cut(s) | 398 |
| PaeR7I | CTCGAG | 1 cut(s) | 1534 |
| PciSI | GCTCTTC | 1 cut(s) | 2163 |
| PctI | GAATGC | 3 cut(s) | 1808, 2263, 2737 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2120 |
| PfeI | GAWTC | 3 cut(s) | 226, 1065, 1957 |
| PflFI | GACNNNGTC | 1 cut(s) | 2389 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1106, 2856 |
| PkrI | GCNGC | 9 cut(s) | 430, 459, 642, 645, 1669, 1926, 2425, 2629, 2772 |
| PleI | GAGTC | 3 cut(s) | 821, 1717, 2410 |
| PpsI | GAGTC | 3 cut(s) | 821, 1717, 2410 |
| PpuMI | RGGWCCY | 2 cut(s) | 217, 1720 |
| PshBI | ATTAAT | 1 cut(s) | 2319 |
| PsiI | TTATAA | 3 cut(s) | 744, 1257, 2502 |
| Psp124BI | GAGCTC | 1 cut(s) | 87 |
| Psp5II | RGGWCCY | 2 cut(s) | 217, 1720 |
| Psp6I | CCWGG | 4 cut(s) | 490, 563, 865, 2773 |
| PspEI | GGTNACC | 1 cut(s) | 1553 |
| PspFI | CCCAGC | 1 cut(s) | 506 |
| PspGI | CCWGG | 4 cut(s) | 490, 563, 865, 2773 |
| PspN4I | GGNNCC | 5 cut(s) | 219, 631, 864, 1368, 1722 |
| PspPI | GGNCC | 3 cut(s) | 217, 499, 1720 |
| PspPPI | RGGWCCY | 2 cut(s) | 217, 1720 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1667 |
| PsyI | GACNNNGTC | 1 cut(s) | 2389 |
| RsaI | GTAC | 4 cut(s) | 942, 1909, 2364, 2453 |
| RsaNI | GTAC | 4 cut(s) | 941, 1908, 2363, 2452 |
| RseI | CAYNNNNRTG | 6 cut(s) | 398, 1002, 1026, 1430, 1874, 2622 |
| SacI | GAGCTC | 1 cut(s) | 87 |
| SapI | GCTCTTC | 1 cut(s) | 2163 |
| SaqAI | TTAA | 6 cut(s) | 1320, 1703, 1803, 2319, 2846, 2944 |
| SatI | GCNGC | 9 cut(s) | 429, 458, 641, 644, 1668, 1925, 2424, 2628, 2771 |
| Sau3AI | GATC | 8 cut(s) | 3, 571, 1096, 1141, 1467, 1489, 2008, 2506 |
| Sau96I | GGNCC | 3 cut(s) | 217, 499, 1720 |
| ScaI | AGTACT | 1 cut(s) | 2364 |
| SchI | GAGTC | 3 cut(s) | 821, 1718, 2411 |
| ScrFI | CCNGG | 6 cut(s) | 492, 556, 565, 867, 1133, 2775 |
| SduI | GDGCHC | 2 cut(s) | 87, 1371 |
| SfaNI | GCATC | 9 cut(s) | 331, 1006, 1030, 1072, 1236, 1539, 1954, 2143, 2410 |
| SfcI | CTRYAG | 2 cut(s) | 147, 760 |
| Sfr274I | CTCGAG | 1 cut(s) | 1534 |
| SfuI | TTCGAA | 1 cut(s) | 1155 |
| SinI | GGWCC | 3 cut(s) | 217, 499, 1720 |
| SlaI | CTCGAG | 1 cut(s) | 1534 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 398, 1002, 1026, 1430, 1874, 2622 |
| SmlI | CTYRAG | 4 cut(s) | 1178, 1286, 1508, 1534 |
| SmoI | CTYRAG | 4 cut(s) | 1178, 1286, 1508, 1534 |
| SsiI | CCGC | 4 cut(s) | 380, 457, 460, 2770 |
| SspMI | CTAG | 5 cut(s) | 410, 1347, 1463, 1656, 2747 |
| SstI | GAGCTC | 1 cut(s) | 87 |
| StyD4I | CCNGG | 6 cut(s) | 490, 554, 563, 865, 1131, 2773 |
| StyI | CCWWGG | 3 cut(s) | 38, 213, 2664 |
| TaaI | ACNGT | 8 cut(s) | 155, 249, 626, 1559, 1614, 2190, 2294, 2389 |
| TaiI | ACGT | 3 cut(s) | 854, 1255, 2614 |
| TaqI | TCGA | 4 cut(s) | 677, 1155, 1535, 2233 |
| TatI | WGTACW | 1 cut(s) | 2362 |
| TauI | GCSGC | 2 cut(s) | 460, 2773 |
| TfiI | GAWTC | 3 cut(s) | 226, 1065, 1957 |
| Tru1I | TTAA | 6 cut(s) | 1320, 1703, 1803, 2319, 2846, 2944 |
| Tru9I | TTAA | 6 cut(s) | 1320, 1703, 1803, 2319, 2846, 2944 |
| TseFI | GTSAC | 4 cut(s) | 391, 1273, 1520, 1553 |
| TseI | GCWGC | 7 cut(s) | 428, 640, 643, 1667, 1924, 2423, 2627 |
| Tsp45I | GTSAC | 4 cut(s) | 391, 1273, 1520, 1553 |
| TspGWI | ACGGA | 2 cut(s) | 1038, 1806 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2389 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1106, 2856 |
| VpaK11BI | GGWCC | 3 cut(s) | 217, 499, 1720 |
| VspI | ATTAAT | 1 cut(s) | 2319 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 733 |
| XapI | RAATTY | 4 cut(s) | 1103, 1235, 1676, 2924 |
| XbaI | TCTAGA | 1 cut(s) | 1346 |
| XceI | RCATGY | 1 cut(s) | 1040 |
| XhoI | CTCGAG | 1 cut(s) | 1534 |
| XmiI | GTMKAC | 2 cut(s) | 1484, 1712 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2120 |
| XspI | CTAG | 5 cut(s) | 410, 1347, 1463, 1656, 2747 |
| ZrmI | AGTACT | 1 cut(s) | 2364 |