Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 759, 1676 |
| AccB1I | GGYRCC | 1 cut(s) | 644 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1118 |
| AccIII | TCCGGA | 1 cut(s) | 1012 |
| AciI | CCGC | 3 cut(s) | 395, 472, 475 |
| AclWI | GGATC | 3 cut(s) | 1103, 1148, 1675 |
| AcsI | RAATTY | 2 cut(s) | 1115, 1247 |
| AcuI | CTGAAG | 2 cut(s) | 113, 893 |
| AfaI | GTAC | 2 cut(s) | 954, 1584 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 570, 750, 1067, 1118, 1326 |
| AgsI | TTSAA | 4 cut(s) | 189, 275, 857, 1284 |
| AjnI | CCWGG | 3 cut(s) | 505, 578, 880 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 969, 1001 |
| AleI | CACNNNNGTG | 1 cut(s) | 413 |
| Alw21I | GWGCWC | 1 cut(s) | 87 |
| Alw26I | GTCTC | 2 cut(s) | 273, 674 |
| AlwI | GGATC | 3 cut(s) | 1103, 1148, 1675 |
| Aor13HI | TCCGGA | 1 cut(s) | 1012 |
| AoxI | GGCC | 3 cut(s) | 427, 907, 1731 |
| ApeKI | GCWGC | 4 cut(s) | 443, 655, 658, 1643 |
| ApoI | RAATTY | 2 cut(s) | 1115, 1247 |
| AseI | ATTAAT | 1 cut(s) | 1539 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1340 |
| AspLEI | GCGC | 3 cut(s) | 27, 1150, 1182 |
| AspS9I | GGNCC | 2 cut(s) | 232, 514 |
| AsuC2I | CCSGG | 2 cut(s) | 571, 1145 |
| AsuHPI | GGTGA | 2 cut(s) | 221, 1001 |
| AsuII | TTCGAA | 1 cut(s) | 1167 |
| AvaII | GGWCC | 2 cut(s) | 232, 514 |
| BanI | GGYRCC | 1 cut(s) | 644 |
| BanII | GRGCYC | 1 cut(s) | 87 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 936, 968 |
| Bbv12I | GWGCWC | 1 cut(s) | 87 |
| BbvI | GCAGC | 4 cut(s) | 430, 667, 670, 1655 |
| BccI | CCATC | 2 cut(s) | 200, 1211 |
| BceAI | ACGGC | 2 cut(s) | 101, 651 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1443, 1477 |
| BciT130I | CCWGG | 3 cut(s) | 507, 580, 882 |
| BciVI | GTATCC | 2 cut(s) | 402, 1566 |
| BcnI | CCSGG | 2 cut(s) | 571, 1145 |
| BcoDI | GTCTC | 2 cut(s) | 273, 674 |
| BfaI | CTAG | 1 cut(s) | 425 |
| BfmI | CTRYAG | 2 cut(s) | 147, 775 |
| BfoI | RGCGCY | 1 cut(s) | 1183 |
| BfuI | GTATCC | 2 cut(s) | 402, 1566 |
| BisI | GCNGC | 5 cut(s) | 444, 473, 656, 659, 1644 |
| BlsI | GCNGC | 5 cut(s) | 445, 474, 657, 660, 1645 |
| BmcAI | AGTACT | 1 cut(s) | 1584 |
| Bme1390I | CCNGG | 5 cut(s) | 507, 571, 580, 882, 1145 |
| Bme18I | GGWCC | 2 cut(s) | 232, 514 |
| BmgT120I | GGNCC | 2 cut(s) | 232, 514 |
| BmiI | GGNNCC | 3 cut(s) | 234, 646, 879 |
| BmrFI | CCNGG | 5 cut(s) | 507, 571, 580, 882, 1145 |
| BmrI | ACTGGG | 1 cut(s) | 808 |
| BmsI | GCATC | 7 cut(s) | 346, 1018, 1042, 1084, 1248, 1363, 1630 |
| BmuI | ACTGGG | 1 cut(s) | 808 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 707, 739, 827, 859 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1647 |
| Bpu14I | TTCGAA | 1 cut(s) | 1167 |
| BpuEI | CTTGAG | 1 cut(s) | 1211 |
| BpuMI | CCSGG | 2 cut(s) | 571, 1145 |
| BsaJI | CCNNGG | 3 cut(s) | 38, 228, 1143 |
| BsaWI | WCCGGW | 1 cut(s) | 1012 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 570, 750, 1067, 1118, 1326 |
| Bse1I | ACTGG | 4 cut(s) | 803, 1000, 1129, 1463 |
| BseAI | TCCGGA | 1 cut(s) | 1012 |
| BseBI | CCWGG | 3 cut(s) | 507, 580, 882 |
| BseDI | CCNNGG | 3 cut(s) | 38, 228, 1143 |
| BseGI | GGATG | 6 cut(s) | 208, 483, 1009, 1021, 1057, 1263 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 570, 750, 1067, 1118, 1326 |
| BseMII | CTCAG | 2 cut(s) | 548, 1593 |
| BseNI | ACTGG | 4 cut(s) | 803, 1000, 1129, 1463 |
| BseRI | GAGGAG | 1 cut(s) | 1562 |
| BseXI | GCAGC | 4 cut(s) | 430, 667, 670, 1655 |
| BseYI | CCCAGC | 1 cut(s) | 521 |
| BsgI | GTGCAG | 2 cut(s) | 1389, 1389 |
| BshFI | GGCC | 3 cut(s) | 429, 909, 1733 |
| BshNI | GGYRCC | 1 cut(s) | 644 |
| BsiHKAI | GWGCWC | 1 cut(s) | 87 |
| BsiSI | CCGG | 3 cut(s) | 570, 1013, 1145 |
| BslFI | GGGAC | 1 cut(s) | 467 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 570, 750, 1067, 1118, 1326 |
| BsmAI | GTCTC | 2 cut(s) | 273, 674 |
| BsmBI | CGTCTC | 1 cut(s) | 674 |
| BsmFI | GGGAC | 1 cut(s) | 467 |
| BsmI | GAATGC | 1 cut(s) | 1483 |
| BsnI | GGCC | 3 cut(s) | 429, 909, 1733 |
| Bsp119I | TTCGAA | 1 cut(s) | 1167 |
| Bsp1286I | GDGCHC | 1 cut(s) | 87 |
| Bsp13I | TCCGGA | 1 cut(s) | 1012 |
| Bsp143I | GATC | 5 cut(s) | 3, 586, 1108, 1153, 1680 |
| Bsp19I | CCATGG | 1 cut(s) | 38 |
| BspACI | CCGC | 3 cut(s) | 395, 472, 475 |
| BspANI | GGCC | 3 cut(s) | 429, 909, 1733 |
| BspCNI | CTCAG | 2 cut(s) | 547, 1592 |
| BspEI | TCCGGA | 1 cut(s) | 1012 |
| BspLI | GGNNCC | 3 cut(s) | 234, 646, 879 |
| BspPI | GGATC | 3 cut(s) | 1103, 1148, 1675 |
| BspQI | GCTCTTC | 1 cut(s) | 1383 |
| BspT104I | TTCGAA | 1 cut(s) | 1167 |
| BspT107I | GGYRCC | 1 cut(s) | 644 |
| BsrI | ACTGG | 4 cut(s) | 803, 1000, 1129, 1463 |
| BssECI | CCNNGG | 3 cut(s) | 38, 228, 1143 |
| BssMI | GATC | 5 cut(s) | 3, 586, 1108, 1153, 1680 |
| BssT1I | CCWWGG | 2 cut(s) | 38, 228 |
| Bst2UI | CCWGG | 3 cut(s) | 507, 580, 882 |
| Bst4CI | ACNGT | 6 cut(s) | 155, 264, 641, 1410, 1514, 1609 |
| Bst6I | CTCTTC | 3 cut(s) | 47, 276, 1383 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1696 |
| BstBI | TTCGAA | 1 cut(s) | 1167 |
| BstC8I | GCNNGC | 1 cut(s) | 1178 |
| BstDEI | CTNAG | 3 cut(s) | 534, 1579, 1647 |
| BstDSI | CCRYGG | 1 cut(s) | 38 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 748 |
| BstF5I | GGATG | 6 cut(s) | 208, 483, 1009, 1021, 1057, 1263 |
| BstH2I | RGCGCY | 1 cut(s) | 1183 |
| BstHHI | GCGC | 3 cut(s) | 27, 1150, 1182 |
| BstKTI | GATC | 5 cut(s) | 6, 589, 1111, 1156, 1683 |
| BstMAI | GTCTC | 2 cut(s) | 273, 674 |
| BstMBI | GATC | 5 cut(s) | 3, 586, 1108, 1153, 1680 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 113, 440, 527, 1649, 1696 |
| BstNI | CCWGG | 3 cut(s) | 507, 580, 882 |
| BstNSI | RCATGY | 1 cut(s) | 1052 |
| BstSCI | CCNGG | 5 cut(s) | 505, 569, 578, 880, 1143 |
| BstSFI | CTRYAG | 2 cut(s) | 147, 775 |
| BstV1I | GCAGC | 4 cut(s) | 430, 667, 670, 1655 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1238 |
| BsuI | GTATCC | 2 cut(s) | 402, 1566 |
| BsuRI | GGCC | 3 cut(s) | 429, 909, 1733 |
| BtgI | CCRYGG | 1 cut(s) | 38 |
| BtsCI | GGATG | 6 cut(s) | 208, 483, 1009, 1021, 1057, 1263 |
| BtsI | GCAGTG | 2 cut(s) | 438, 1401 |
| BtsIMutI | CAGTG | 7 cut(s) | 160, 438, 1072, 1209, 1401, 1456, 1574 |
| Cac8I | GCNNGC | 1 cut(s) | 1178 |
| CfoI | GCGC | 3 cut(s) | 27, 1150, 1182 |
| Cfr13I | GGNCC | 2 cut(s) | 232, 514 |
| Csp6I | GTAC | 2 cut(s) | 953, 1583 |
| CspCI | CAANNNNNGTGG | 6 cut(s) | 285, 320, 714, 749, 1345, 1380 |
| CviQI | GTAC | 2 cut(s) | 953, 1583 |
| DdeI | CTNAG | 3 cut(s) | 534, 1579, 1647 |
| DpnI | GATC | 5 cut(s) | 5, 588, 1110, 1155, 1682 |
| DpnII | GATC | 5 cut(s) | 3, 586, 1108, 1153, 1680 |
| Eam1104I | CTCTTC | 3 cut(s) | 47, 276, 1383 |
| EarI | CTCTTC | 3 cut(s) | 47, 276, 1383 |
| EciI | GGCGGA | 2 cut(s) | 384, 490 |
| Ecl136II | GAGCTC | 1 cut(s) | 85 |
| Eco130I | CCWWGG | 2 cut(s) | 38, 228 |
| Eco24I | GRGCYC | 1 cut(s) | 87 |
| Eco32I | GATATC | 1 cut(s) | 1451 |
| Eco47I | GGWCC | 2 cut(s) | 232, 514 |
| Eco53kI | GAGCTC | 1 cut(s) | 85 |
| Eco57I | CTGAAG | 2 cut(s) | 113, 893 |
| EcoICRI | GAGCTC | 1 cut(s) | 85 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 748 |
| EcoO109I | RGGNCCY | 1 cut(s) | 232 |
| EcoRII | CCWGG | 3 cut(s) | 505, 578, 880 |
| EcoRV | GATATC | 1 cut(s) | 1451 |
| EcoT14I | CCWWGG | 2 cut(s) | 38, 228 |
| EcoT38I | GRGCYC | 1 cut(s) | 87 |
| ErhI | CCWWGG | 2 cut(s) | 38, 228 |
| Esp3I | CGTCTC | 1 cut(s) | 674 |
| FaqI | GGGAC | 1 cut(s) | 467 |
| Fnu4HI | GCNGC | 5 cut(s) | 444, 473, 656, 659, 1644 |
| FokI | GGATG | 6 cut(s) | 215, 490, 996, 1028, 1044, 1270 |
| FriOI | GRGCYC | 1 cut(s) | 87 |
| Fsp4HI | GCNGC | 5 cut(s) | 444, 473, 656, 659, 1644 |
| FspBI | CTAG | 1 cut(s) | 425 |
| GlaI | GCGC | 3 cut(s) | 26, 1149, 1181 |
| GluI | GCNGC | 5 cut(s) | 444, 473, 656, 659, 1644 |
| GsaI | CCCAGC | 1 cut(s) | 525 |
| HaeII | RGCGCY | 1 cut(s) | 1183 |
| HaeIII | GGCC | 3 cut(s) | 429, 909, 1733 |
| HapII | CCGG | 3 cut(s) | 570, 1013, 1145 |
| HhaI | GCGC | 3 cut(s) | 27, 1150, 1182 |
| Hin6I | GCGC | 3 cut(s) | 25, 1148, 1180 |
| HinP1I | GCGC | 3 cut(s) | 25, 1148, 1180 |
| HincII | GTYRAC | 1 cut(s) | 796 |
| HindII | GTYRAC | 1 cut(s) | 796 |
| HindIII | AAGCTT | 3 cut(s) | 125, 1100, 1277 |
| HinfI | GANTC | 4 cut(s) | 241, 842, 1077, 1622 |
| HpaII | CCGG | 3 cut(s) | 570, 1013, 1145 |
| HphI | GGTGA | 2 cut(s) | 221, 1001 |
| Hpy166II | GTNNAC | 3 cut(s) | 796, 841, 1268 |
| Hpy188I | TCNGA | 6 cut(s) | 122, 402, 565, 829, 1414, 1639 |
| Hpy188III | TCNNGA | 9 cut(s) | 131, 848, 1013, 1112, 1190, 1427, 1435, 1544, 1723 |
| Hpy8I | GTNNAC | 3 cut(s) | 796, 841, 1268 |
| Hpy99I | CGWCG | 1 cut(s) | 1514 |
| HpyAV | CCTTC | 6 cut(s) | 51, 157, 855, 868, 938, 1319 |
| HpyCH4III | ACNGT | 6 cut(s) | 155, 264, 641, 1410, 1514, 1609 |
| HpyCH4IV | ACGT | 3 cut(s) | 466, 866, 1264 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 113, 440, 527, 1649, 1696 |
| HpyF3I | CTNAG | 3 cut(s) | 534, 1579, 1647 |
| HpySE526I | ACGT | 3 cut(s) | 466, 866, 1264 |
| HspAI | GCGC | 3 cut(s) | 25, 1148, 1180 |
| Kpn2I | TCCGGA | 1 cut(s) | 1012 |
| Kzo9I | GATC | 5 cut(s) | 3, 586, 1108, 1153, 1680 |
| LguI | GCTCTTC | 1 cut(s) | 1383 |
| LmnI | GCTCC | 3 cut(s) | 371, 812, 1549 |
| Lsp1109I | GCAGC | 4 cut(s) | 430, 667, 670, 1655 |
| LweI | GCATC | 7 cut(s) | 346, 1018, 1042, 1084, 1248, 1363, 1630 |
| MaeI | CTAG | 1 cut(s) | 425 |
| MaeII | ACGT | 3 cut(s) | 466, 866, 1264 |
| MaeIII | GTNAC | 2 cut(s) | 406, 867 |
| MalI | GATC | 5 cut(s) | 5, 588, 1110, 1155, 1682 |
| MboI | GATC | 5 cut(s) | 3, 586, 1108, 1153, 1680 |
| MfeI | CAATTG | 1 cut(s) | 1596 |
| MhlI | GDGCHC | 1 cut(s) | 87 |
| MluCI | AATT | 5 cut(s) | 831, 1115, 1247, 1536, 1596 |
| MlyI | GAGTC | 2 cut(s) | 836, 1631 |
| MmeI | TCCRAC | 2 cut(s) | 219, 1708 |
| MroI | TCCGGA | 1 cut(s) | 1012 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1340 |
| MseI | TTAA | 1 cut(s) | 1539 |
| MslI | CAYNNNNRTG | 3 cut(s) | 413, 1014, 1038 |
| MspI | CCGG | 3 cut(s) | 570, 1013, 1145 |
| MspR9I | CCNGG | 5 cut(s) | 507, 571, 580, 882, 1145 |
| MunI | CAATTG | 1 cut(s) | 1596 |
| Mva1269I | GAATGC | 1 cut(s) | 1483 |
| MvaI | CCWGG | 3 cut(s) | 507, 580, 882 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 113, 440, 527, 1649, 1696 |
| NciI | CCSGG | 2 cut(s) | 571, 1145 |
| NcoI | CCATGG | 1 cut(s) | 38 |
| NdeII | GATC | 5 cut(s) | 3, 586, 1108, 1153, 1680 |
| NlaIV | GGNNCC | 3 cut(s) | 234, 646, 879 |
| NmuCI | GTSAC | 1 cut(s) | 406 |
| NspI | RCATGY | 1 cut(s) | 1052 |
| NspV | TTCGAA | 1 cut(s) | 1167 |
| OliI | CACNNNNGTG | 1 cut(s) | 413 |
| PciSI | GCTCTTC | 1 cut(s) | 1383 |
| PctI | GAATGC | 1 cut(s) | 1483 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1340 |
| PfeI | GAWTC | 2 cut(s) | 241, 1077 |
| PflFI | GACNNNGTC | 1 cut(s) | 1609 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1118 |
| PkrI | GCNGC | 5 cut(s) | 445, 474, 657, 660, 1645 |
| PleI | GAGTC | 2 cut(s) | 836, 1630 |
| PpsI | GAGTC | 2 cut(s) | 836, 1630 |
| PpuMI | RGGWCCY | 1 cut(s) | 232 |
| PshBI | ATTAAT | 1 cut(s) | 1539 |
| PsiI | TTATAA | 2 cut(s) | 759, 1676 |
| Psp124BI | GAGCTC | 1 cut(s) | 87 |
| Psp5II | RGGWCCY | 1 cut(s) | 232 |
| Psp6I | CCWGG | 3 cut(s) | 505, 578, 880 |
| PspFI | CCCAGC | 1 cut(s) | 521 |
| PspGI | CCWGG | 3 cut(s) | 505, 578, 880 |
| PspN4I | GGNNCC | 3 cut(s) | 234, 646, 879 |
| PspPI | GGNCC | 2 cut(s) | 232, 514 |
| PspPPI | RGGWCCY | 1 cut(s) | 232 |
| PsyI | GACNNNGTC | 1 cut(s) | 1609 |
| RsaI | GTAC | 2 cut(s) | 954, 1584 |
| RsaNI | GTAC | 2 cut(s) | 953, 1583 |
| RseI | CAYNNNNRTG | 3 cut(s) | 413, 1014, 1038 |
| SacI | GAGCTC | 1 cut(s) | 87 |
| SapI | GCTCTTC | 1 cut(s) | 1383 |
| SaqAI | TTAA | 1 cut(s) | 1539 |
| SatI | GCNGC | 5 cut(s) | 444, 473, 656, 659, 1644 |
| Sau3AI | GATC | 5 cut(s) | 3, 586, 1108, 1153, 1680 |
| Sau96I | GGNCC | 2 cut(s) | 232, 514 |
| ScaI | AGTACT | 1 cut(s) | 1584 |
| SchI | GAGTC | 2 cut(s) | 836, 1631 |
| ScrFI | CCNGG | 5 cut(s) | 507, 571, 580, 882, 1145 |
| SduI | GDGCHC | 1 cut(s) | 87 |
| SfaNI | GCATC | 7 cut(s) | 346, 1018, 1042, 1084, 1248, 1363, 1630 |
| SfcI | CTRYAG | 2 cut(s) | 147, 775 |
| SfuI | TTCGAA | 1 cut(s) | 1167 |
| SinI | GGWCC | 2 cut(s) | 232, 514 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 413, 1014, 1038 |
| SmlI | CTYRAG | 1 cut(s) | 1190 |
| SmoI | CTYRAG | 1 cut(s) | 1190 |
| Sse9I | AATT | 5 cut(s) | 831, 1115, 1247, 1536, 1596 |
| SsiI | CCGC | 3 cut(s) | 395, 472, 475 |
| SspMI | CTAG | 1 cut(s) | 425 |
| SstI | GAGCTC | 1 cut(s) | 87 |
| StyD4I | CCNGG | 5 cut(s) | 505, 569, 578, 880, 1143 |
| StyI | CCWWGG | 2 cut(s) | 38, 228 |
| TaaI | ACNGT | 6 cut(s) | 155, 264, 641, 1410, 1514, 1609 |
| TaiI | ACGT | 3 cut(s) | 469, 869, 1267 |
| TaqI | TCGA | 3 cut(s) | 692, 1167, 1453 |
| TasI | AATT | 5 cut(s) | 831, 1115, 1247, 1536, 1596 |
| TatI | WGTACW | 1 cut(s) | 1582 |
| TauI | GCSGC | 1 cut(s) | 475 |
| TfiI | GAWTC | 2 cut(s) | 241, 1077 |
| Tru1I | TTAA | 1 cut(s) | 1539 |
| Tru9I | TTAA | 1 cut(s) | 1539 |
| TscAI | CASTG | 7 cut(s) | 160, 445, 1072, 1216, 1408, 1463, 1574 |
| TseFI | GTSAC | 1 cut(s) | 406 |
| TseI | GCWGC | 4 cut(s) | 443, 655, 658, 1643 |
| Tsp45I | GTSAC | 1 cut(s) | 406 |
| TspDTI | ATGAA | 4 cut(s) | 410, 669, 1028, 1046 |
| TspGWI | ACGGA | 1 cut(s) | 1050 |
| TspRI | CASTG | 7 cut(s) | 160, 445, 1072, 1216, 1408, 1463, 1574 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1609 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1118 |
| VpaK11BI | GGWCC | 2 cut(s) | 232, 514 |
| VspI | ATTAAT | 1 cut(s) | 1539 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 748 |
| XapI | RAATTY | 2 cut(s) | 1115, 1247 |
| XceI | RCATGY | 1 cut(s) | 1052 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1340 |
| XspI | CTAG | 1 cut(s) | 425 |
| ZrmI | AGTACT | 1 cut(s) | 1584 |