Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 2 cut(s) | 371, 659 |
| AccI | GTMKAC | 1 cut(s) | 292 |
| AccIII | TCCGGA | 1 cut(s) | 38 |
| AclWI | GGATC | 1 cut(s) | 745 |
| AcsI | RAATTY | 3 cut(s) | 318, 655, 952 |
| AcuI | CTGAAG | 2 cut(s) | 708, 761 |
| AfaI | GTAC | 2 cut(s) | 464, 479 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 371, 587, 659 |
| AflII | CTTAAG | 1 cut(s) | 452 |
| AgsI | TTSAA | 4 cut(s) | 501, 842, 886, 925 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 747, 779 |
| AluBI | AGCT | 6 cut(s) | 162, 451, 469, 613, 676, 874 |
| AluI | AGCT | 6 cut(s) | 162, 451, 469, 613, 676, 874 |
| Alw21I | GWGCWC | 2 cut(s) | 615, 678 |
| Alw26I | GTCTC | 1 cut(s) | 750 |
| AlwI | GGATC | 1 cut(s) | 745 |
| Aor13HI | TCCGGA | 1 cut(s) | 38 |
| AoxI | GGCC | 1 cut(s) | 9 |
| ApeKI | GCWGC | 1 cut(s) | 246 |
| ApoI | RAATTY | 3 cut(s) | 318, 655, 952 |
| AspLEI | GCGC | 1 cut(s) | 475 |
| AspS9I | GGNCC | 3 cut(s) | 10, 569, 641 |
| AsuHPI | GGTGA | 2 cut(s) | 271, 818 |
| AvaII | GGWCC | 2 cut(s) | 569, 641 |
| BanII | GRGCYC | 2 cut(s) | 615, 678 |
| Bbv12I | GWGCWC | 2 cut(s) | 615, 678 |
| BbvI | GCAGC | 1 cut(s) | 258 |
| BccI | CCATC | 1 cut(s) | 144 |
| BceAI | ACGGC | 3 cut(s) | 24, 37, 88 |
| BcoDI | GTCTC | 1 cut(s) | 750 |
| BfaI | CTAG | 5 cut(s) | 117, 213, 296, 731, 875 |
| BfmI | CTRYAG | 1 cut(s) | 902 |
| BfrI | CTTAAG | 1 cut(s) | 452 |
| BglII | AGATCT | 1 cut(s) | 898 |
| BisI | GCNGC | 1 cut(s) | 247 |
| BlsI | GCNGC | 1 cut(s) | 248 |
| Bme18I | GGWCC | 2 cut(s) | 569, 641 |
| BmgT120I | GGNCC | 3 cut(s) | 10, 569, 641 |
| BmiI | GGNNCC | 2 cut(s) | 571, 859 |
| BmsI | GCATC | 2 cut(s) | 218, 613 |
| BpmI | CTGGAG | 2 cut(s) | 564, 876 |
| BpuEI | CTTGAG | 2 cut(s) | 410, 420 |
| Bsa29I | ATCGAT | 1 cut(s) | 621 |
| BsaJI | CCNNGG | 1 cut(s) | 135 |
| BsaWI | WCCGGW | 2 cut(s) | 38, 287 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 371, 587, 659 |
| Bse1I | ACTGG | 1 cut(s) | 238 |
| Bse3DI | GCAATG | 1 cut(s) | 229 |
| BseAI | TCCGGA | 1 cut(s) | 38 |
| BseCI | ATCGAT | 1 cut(s) | 621 |
| BseDI | CCNNGG | 1 cut(s) | 135 |
| BseGI | GGATG | 1 cut(s) | 357 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 371, 587, 659 |
| BseMI | GCAATG | 1 cut(s) | 229 |
| BseMII | CTCAG | 1 cut(s) | 103 |
| BseNI | ACTGG | 1 cut(s) | 238 |
| BseXI | GCAGC | 1 cut(s) | 258 |
| BsgI | GTGCAG | 1 cut(s) | 383 |
| BshFI | GGCC | 1 cut(s) | 11 |
| BshVI | ATCGAT | 1 cut(s) | 621 |
| BsiHKAI | GWGCWC | 2 cut(s) | 615, 678 |
| BsiSI | CCGG | 2 cut(s) | 39, 288 |
| BslFI | GGGAC | 1 cut(s) | 555 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 371, 587, 659 |
| BsmAI | GTCTC | 1 cut(s) | 750 |
| BsmFI | GGGAC | 1 cut(s) | 555 |
| BsnI | GGCC | 1 cut(s) | 11 |
| Bsp1286I | GDGCHC | 2 cut(s) | 615, 678 |
| Bsp13I | TCCGGA | 1 cut(s) | 38 |
| Bsp1407I | TGTACA | 1 cut(s) | 477 |
| Bsp143I | GATC | 4 cut(s) | 298, 328, 750, 898 |
| BspANI | GGCC | 1 cut(s) | 11 |
| BspCNI | CTCAG | 1 cut(s) | 102 |
| BspDI | ATCGAT | 1 cut(s) | 621 |
| BspEI | TCCGGA | 1 cut(s) | 38 |
| BspLI | GGNNCC | 2 cut(s) | 571, 859 |
| BspPI | GGATC | 1 cut(s) | 745 |
| BspQI | GCTCTTC | 1 cut(s) | 147 |
| BspTI | CTTAAG | 1 cut(s) | 452 |
| BsrDI | GCAATG | 1 cut(s) | 229 |
| BsrGI | TGTACA | 1 cut(s) | 477 |
| BsrI | ACTGG | 1 cut(s) | 238 |
| BssECI | CCNNGG | 1 cut(s) | 135 |
| BssMI | GATC | 4 cut(s) | 298, 328, 750, 898 |
| BssNAI | GTATAC | 1 cut(s) | 293 |
| Bst1107I | GTATAC | 1 cut(s) | 293 |
| Bst4CI | ACNGT | 2 cut(s) | 682, 906 |
| Bst6I | CTCTTC | 1 cut(s) | 147 |
| BstAFI | CTTAAG | 1 cut(s) | 452 |
| BstAUI | TGTACA | 1 cut(s) | 477 |
| BstC8I | GCNNGC | 2 cut(s) | 160, 471 |
| BstDEI | CTNAG | 1 cut(s) | 89 |
| BstEII | GGTNACC | 1 cut(s) | 806 |
| BstF5I | GGATG | 1 cut(s) | 357 |
| BstHHI | GCGC | 1 cut(s) | 475 |
| BstKTI | GATC | 4 cut(s) | 301, 331, 753, 901 |
| BstMAI | GTCTC | 1 cut(s) | 750 |
| BstMBI | GATC | 4 cut(s) | 298, 328, 750, 898 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 113, 525 |
| BstPI | GGTNACC | 1 cut(s) | 806 |
| BstSFI | CTRYAG | 1 cut(s) | 902 |
| BstV1I | GCAGC | 1 cut(s) | 258 |
| BstX2I | RGATCY | 1 cut(s) | 898 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 422, 566 |
| BstYI | RGATCY | 1 cut(s) | 898 |
| BstZ17I | GTATAC | 1 cut(s) | 293 |
| Bsu15I | ATCGAT | 1 cut(s) | 621 |
| BsuRI | GGCC | 1 cut(s) | 11 |
| BsuTUI | ATCGAT | 1 cut(s) | 621 |
| BtsCI | GGATG | 1 cut(s) | 357 |
| BtsI | GCAGTG | 1 cut(s) | 249 |
| BtsIMutI | CAGTG | 2 cut(s) | 249, 678 |
| Cac8I | GCNNGC | 2 cut(s) | 160, 471 |
| CfoI | GCGC | 1 cut(s) | 475 |
| Cfr13I | GGNCC | 3 cut(s) | 10, 569, 641 |
| ClaI | ATCGAT | 1 cut(s) | 621 |
| Csp6I | GTAC | 2 cut(s) | 463, 478 |
| CviAII | CATG | 5 cut(s) | 195, 219, 460, 532, 913 |
| CviQI | GTAC | 2 cut(s) | 463, 478 |
| DdeI | CTNAG | 1 cut(s) | 89 |
| DpnI | GATC | 4 cut(s) | 300, 330, 752, 900 |
| DpnII | GATC | 4 cut(s) | 298, 328, 750, 898 |
| DraI | TTTAAA | 1 cut(s) | 420 |
| Eam1104I | CTCTTC | 1 cut(s) | 147 |
| EarI | CTCTTC | 1 cut(s) | 147 |
| Ecl136II | GAGCTC | 2 cut(s) | 613, 676 |
| Eco24I | GRGCYC | 2 cut(s) | 615, 678 |
| Eco47I | GGWCC | 2 cut(s) | 569, 641 |
| Eco53kI | GAGCTC | 2 cut(s) | 613, 676 |
| Eco57I | CTGAAG | 2 cut(s) | 708, 761 |
| Eco91I | GGTNACC | 1 cut(s) | 806 |
| EcoICRI | GAGCTC | 2 cut(s) | 613, 676 |
| EcoO65I | GGTNACC | 1 cut(s) | 806 |
| EcoT22I | ATGCAT | 1 cut(s) | 211 |
| EcoT38I | GRGCYC | 2 cut(s) | 615, 678 |
| FaeI | CATG | 5 cut(s) | 198, 222, 463, 535, 916 |
| FaqI | GGGAC | 1 cut(s) | 555 |
| FatI | CATG | 5 cut(s) | 194, 218, 459, 531, 912 |
| FblI | GTMKAC | 1 cut(s) | 292 |
| Fnu4HI | GCNGC | 1 cut(s) | 247 |
| FokI | GGATG | 1 cut(s) | 344 |
| FriOI | GRGCYC | 2 cut(s) | 615, 678 |
| Fsp4HI | GCNGC | 1 cut(s) | 247 |
| FspBI | CTAG | 5 cut(s) | 117, 213, 296, 731, 875 |
| GlaI | GCGC | 1 cut(s) | 474 |
| GluI | GCNGC | 1 cut(s) | 247 |
| GsuI | CTGGAG | 2 cut(s) | 564, 876 |
| HaeIII | GGCC | 1 cut(s) | 11 |
| HapII | CCGG | 2 cut(s) | 39, 288 |
| HhaI | GCGC | 1 cut(s) | 475 |
| Hin1II | CATG | 5 cut(s) | 198, 222, 463, 535, 916 |
| Hin6I | GCGC | 1 cut(s) | 473 |
| HinP1I | GCGC | 1 cut(s) | 473 |
| HincII | GTYRAC | 2 cut(s) | 265, 909 |
| HindII | GTYRAC | 2 cut(s) | 265, 909 |
| HinfI | GANTC | 2 cut(s) | 87, 916 |
| HpaII | CCGG | 2 cut(s) | 39, 288 |
| HphI | GGTGA | 2 cut(s) | 271, 818 |
| Hpy166II | GTNNAC | 4 cut(s) | 265, 293, 480, 909 |
| Hpy188I | TCNGA | 7 cut(s) | 70, 92, 149, 727, 741, 750, 771 |
| Hpy188III | TCNNGA | 8 cut(s) | 39, 326, 389, 437, 491, 581, 793, 855 |
| Hpy8I | GTNNAC | 4 cut(s) | 265, 293, 480, 909 |
| Hpy99I | CGWCG | 2 cut(s) | 10, 74 |
| HpyAV | CCTTC | 3 cut(s) | 488, 605, 732 |
| HpyCH4III | ACNGT | 2 cut(s) | 682, 906 |
| HpyCH4V | TGCA | 4 cut(s) | 209, 222, 246, 400 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 113, 525 |
| HpyF3I | CTNAG | 1 cut(s) | 89 |
| Hsp92II | CATG | 5 cut(s) | 198, 222, 463, 535, 916 |
| HspAI | GCGC | 1 cut(s) | 473 |
| Kpn2I | TCCGGA | 1 cut(s) | 38 |
| Kzo9I | GATC | 4 cut(s) | 298, 328, 750, 898 |
| LguI | GCTCTTC | 1 cut(s) | 147 |
| LmnI | GCTCC | 1 cut(s) | 857 |
| Lsp1109I | GCAGC | 1 cut(s) | 258 |
| LweI | GCATC | 2 cut(s) | 218, 613 |
| MaeI | CTAG | 5 cut(s) | 117, 213, 296, 731, 875 |
| MaeIII | GTNAC | 3 cut(s) | 695, 806, 983 |
| MalI | GATC | 4 cut(s) | 300, 330, 752, 900 |
| MboI | GATC | 4 cut(s) | 298, 328, 750, 898 |
| MboII | GAAGA | 2 cut(s) | 102, 134 |
| MfeI | CAATTG | 1 cut(s) | 920 |
| MflI | RGATCY | 1 cut(s) | 898 |
| MhlI | GDGCHC | 2 cut(s) | 615, 678 |
| MlyI | GAGTC | 1 cut(s) | 81 |
| MmeI | TCCRAC | 2 cut(s) | 93, 778 |
| Mph1103I | ATGCAT | 1 cut(s) | 211 |
| MroI | TCCGGA | 1 cut(s) | 38 |
| MseI | TTAA | 3 cut(s) | 380, 419, 453 |
| MslI | CAYNNNNRTG | 2 cut(s) | 420, 683 |
| MspCI | CTTAAG | 1 cut(s) | 452 |
| MspI | CCGG | 2 cut(s) | 39, 288 |
| MunI | CAATTG | 1 cut(s) | 920 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 113, 525 |
| NdeII | GATC | 4 cut(s) | 298, 328, 750, 898 |
| NlaIII | CATG | 5 cut(s) | 198, 222, 463, 535, 916 |
| NlaIV | GGNNCC | 2 cut(s) | 571, 859 |
| NmeAIII | GCCGAG | 1 cut(s) | 495 |
| NmuCI | GTSAC | 2 cut(s) | 806, 983 |
| NsiI | ATGCAT | 1 cut(s) | 211 |
| PciSI | GCTCTTC | 1 cut(s) | 147 |
| PfeI | GAWTC | 1 cut(s) | 916 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 371, 659 |
| PkrI | GCNGC | 1 cut(s) | 248 |
| PleI | GAGTC | 1 cut(s) | 81 |
| PpsI | GAGTC | 1 cut(s) | 81 |
| Psp124BI | GAGCTC | 2 cut(s) | 615, 678 |
| PspEI | GGTNACC | 1 cut(s) | 806 |
| PspN4I | GGNNCC | 2 cut(s) | 571, 859 |
| PspPI | GGNCC | 3 cut(s) | 10, 569, 641 |
| PsuI | RGATCY | 1 cut(s) | 898 |
| RsaI | GTAC | 2 cut(s) | 464, 479 |
| RsaNI | GTAC | 2 cut(s) | 463, 478 |
| RseI | CAYNNNNRTG | 2 cut(s) | 420, 683 |
| SacI | GAGCTC | 2 cut(s) | 615, 678 |
| SapI | GCTCTTC | 1 cut(s) | 147 |
| SaqAI | TTAA | 3 cut(s) | 380, 419, 453 |
| SatI | GCNGC | 1 cut(s) | 247 |
| Sau3AI | GATC | 4 cut(s) | 298, 328, 750, 898 |
| Sau96I | GGNCC | 3 cut(s) | 10, 569, 641 |
| SchI | GAGTC | 1 cut(s) | 81 |
| SduI | GDGCHC | 2 cut(s) | 615, 678 |
| SfaNI | GCATC | 2 cut(s) | 218, 613 |
| SfcI | CTRYAG | 1 cut(s) | 902 |
| SinI | GGWCC | 2 cut(s) | 569, 641 |
| SmiI | ATTTAAAT | 1 cut(s) | 420 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 420, 683 |
| SmlI | CTYRAG | 3 cut(s) | 389, 435, 452 |
| SmoI | CTYRAG | 3 cut(s) | 389, 435, 452 |
| SspMI | CTAG | 5 cut(s) | 117, 213, 296, 731, 875 |
| SstI | GAGCTC | 2 cut(s) | 615, 678 |
| SwaI | ATTTAAAT | 1 cut(s) | 420 |
| TaaI | ACNGT | 2 cut(s) | 682, 906 |
| TaqI | TCGA | 3 cut(s) | 85, 492, 621 |
| TaqII | GACCGA | 1 cut(s) | 20 |
| TatI | WGTACW | 1 cut(s) | 477 |
| TfiI | GAWTC | 1 cut(s) | 916 |
| Tru1I | TTAA | 3 cut(s) | 380, 419, 453 |
| Tru9I | TTAA | 3 cut(s) | 380, 419, 453 |
| TscAI | CASTG | 2 cut(s) | 256, 685 |
| TseFI | GTSAC | 2 cut(s) | 806, 983 |
| TseI | GCWGC | 1 cut(s) | 246 |
| Tsp45I | GTSAC | 2 cut(s) | 806, 983 |
| TspDTI | ATGAA | 7 cut(s) | 448, 567, 587, 658, 755, 929, 933 |
| TspGWI | ACGGA | 1 cut(s) | 971 |
| TspRI | CASTG | 2 cut(s) | 256, 685 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 371, 659 |
| Vha464I | CTTAAG | 1 cut(s) | 452 |
| VpaK11BI | GGWCC | 2 cut(s) | 569, 641 |
| XapI | RAATTY | 3 cut(s) | 318, 655, 952 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 225 |
| XmiI | GTMKAC | 1 cut(s) | 292 |
| XspI | CTAG | 5 cut(s) | 117, 213, 296, 731, 875 |
| Zsp2I | ATGCAT | 1 cut(s) | 211 |