Belongs to the class-I aminoacyl-tRNA synthetase family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1553 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 803 |
| Acc16I | TGCGCA | 1 cut(s) | 529 |
| Acc36I | ACCTGC | 2 cut(s) | 705, 1553 |
| AccB1I | GGYRCC | 2 cut(s) | 960, 1069 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 970 |
| AccBSI | CCGCTC | 2 cut(s) | 174, 460 |
| AccI | GTMKAC | 1 cut(s) | 807 |
| AccII | CGCG | 9 cut(s) | 186, 199, 517, 551, 731, 815, 956, 1041, 1075 |
| AclWI | GGATC | 7 cut(s) | 253, 373, 403, 578, 935, 1005, 2114 |
| AcoI | YGGCCR | 4 cut(s) | 455, 879, 1018, 1076 |
| AcsI | RAATTY | 3 cut(s) | 253, 1669, 2125 |
| AcuI | CTGAAG | 2 cut(s) | 1724, 1725 |
| AcyI | GRCGYC | 5 cut(s) | 738, 786, 801, 1070, 1865 |
| AfaI | GTAC | 5 cut(s) | 643, 665, 902, 938, 2140 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 297, 352, 586, 612, 747, 970, 991, 1411, 1831 |
| AflIII | ACRYGT | 1 cut(s) | 954 |
| AgsI | TTSAA | 7 cut(s) | 1115, 1265, 1425, 1462, 1713, 1849, 1910 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1176 |
| AjiI | CACGTC | 2 cut(s) | 895, 1063 |
| AjnI | CCWGG | 3 cut(s) | 123, 1395, 1656 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2073, 2105 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1527, 1559 |
| Alw21I | GWGCWC | 3 cut(s) | 701, 928, 1507 |
| Alw26I | GTCTC | 6 cut(s) | 394, 702, 720, 1583, 1937, 2185 |
| AlwI | GGATC | 7 cut(s) | 253, 373, 403, 578, 935, 1005, 2114 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 970 |
| AoxI | GGCC | 9 cut(s) | 126, 455, 869, 879, 915, 1018, 1076, 1821, 1978 |
| ApeKI | GCWGC | 8 cut(s) | 79, 82, 85, 88, 483, 530, 1559, 1968 |
| ApoI | RAATTY | 3 cut(s) | 253, 1669, 2125 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 786, 818 |
| AspLEI | GCGC | 5 cut(s) | 186, 201, 530, 551, 1072 |
| AsuC2I | CCSGG | 7 cut(s) | 102, 110, 281, 353, 454, 859, 945 |
| AsuHPI | GGTGA | 5 cut(s) | 601, 781, 1677, 1757, 1847 |
| AsuII | TTCGAA | 1 cut(s) | 251 |
| AvaII | GGWCC | 8 cut(s) | 112, 322, 497, 576, 619, 1393, 1399, 1654 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 959, 992, 1682, 1715 |
| BanI | GGYRCC | 2 cut(s) | 960, 1069 |
| BanII | GRGCYC | 4 cut(s) | 304, 701, 928, 1507 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1799, 1831 |
| BauI | CACGAG | 2 cut(s) | 175, 478 |
| BbsI | GAAGAC | 1 cut(s) | 1443 |
| Bbv12I | GWGCWC | 3 cut(s) | 701, 928, 1507 |
| BbvI | GCAGC | 8 cut(s) | 66, 94, 97, 100, 495, 542, 1546, 1980 |
| BccI | CCATC | 4 cut(s) | 666, 1604, 1742, 1926 |
| BceAI | ACGGC | 5 cut(s) | 725, 727, 814, 1005, 1087 |
| BciT130I | CCWGG | 3 cut(s) | 125, 1397, 1658 |
| BciVI | GTATCC | 2 cut(s) | 1155, 2024 |
| BcnI | CCSGG | 7 cut(s) | 102, 110, 281, 353, 454, 859, 945 |
| BcoDI | GTCTC | 6 cut(s) | 394, 702, 720, 1583, 1937, 2185 |
| BfaI | CTAG | 4 cut(s) | 140, 645, 1209, 1547 |
| BfmI | CTRYAG | 3 cut(s) | 80, 1560, 2162 |
| BfoI | RGCGCY | 1 cut(s) | 1073 |
| BfuAI | ACCTGC | 2 cut(s) | 705, 1553 |
| BfuI | GTATCC | 2 cut(s) | 1155, 2024 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 1108, 1977 |
| BmcAI | AGTACT | 2 cut(s) | 643, 665 |
| Bme18I | GGWCC | 8 cut(s) | 112, 322, 497, 576, 619, 1393, 1399, 1654 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1176 |
| BmgBI | CACGTC | 2 cut(s) | 895, 1063 |
| BmrI | ACTGGG | 1 cut(s) | 1168 |
| BmsI | GCATC | 2 cut(s) | 404, 1179 |
| BmuI | ACTGGG | 1 cut(s) | 1168 |
| BpiI | GAAGAC | 1 cut(s) | 1443 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 744, 776 |
| BpmI | CTGGAG | 3 cut(s) | 769, 992, 2025 |
| Bpu10I | CCTNAGC | 1 cut(s) | 623 |
| Bpu14I | TTCGAA | 1 cut(s) | 251 |
| BpuEI | CTTGAG | 1 cut(s) | 2003 |
| BpuMI | CCSGG | 7 cut(s) | 102, 110, 281, 353, 454, 859, 945 |
| BsaAI | YACGTR | 2 cut(s) | 559, 904 |
| BsaBI | GATNNNNATC | 3 cut(s) | 1130, 1712, 2200 |
| BsaHI | GRCGYC | 5 cut(s) | 738, 786, 801, 1070, 1865 |
| BsaJI | CCNNGG | 8 cut(s) | 123, 452, 882, 918, 944, 1039, 1073, 1245 |
| BsaWI | WCCGGW | 1 cut(s) | 16 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 668, 698 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 297, 352, 586, 612, 747, 970, 991, 1411, 1831 |
| Bse118I | RCCGGY | 1 cut(s) | 871 |
| Bse1I | ACTGG | 7 cut(s) | 262, 975, 1174, 1208, 1382, 1445, 2042 |
| Bse8I | GATNNNNATC | 3 cut(s) | 1130, 1712, 2200 |
| BseBI | CCWGG | 3 cut(s) | 125, 1397, 1658 |
| BseDI | CCNNGG | 8 cut(s) | 123, 452, 882, 918, 944, 1039, 1073, 1245 |
| BseGI | GGATG | 3 cut(s) | 677, 1096, 1194 |
| BseJI | GATNNNNATC | 3 cut(s) | 1130, 1712, 2200 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 297, 352, 586, 612, 747, 970, 991, 1411, 1831 |
| BseMII | CTCAG | 5 cut(s) | 487, 614, 920, 1209, 2039 |
| BseNI | ACTGG | 7 cut(s) | 262, 975, 1174, 1208, 1382, 1445, 2042 |
| BseRI | GAGGAG | 1 cut(s) | 404 |
| BseX3I | CGGCCG | 3 cut(s) | 455, 1018, 1076 |
| BseXI | GCAGC | 8 cut(s) | 66, 94, 97, 100, 495, 542, 1546, 1980 |
| Bsh1236I | CGCG | 9 cut(s) | 186, 199, 517, 551, 731, 815, 956, 1041, 1075 |
| Bsh1285I | CGRYCG | 5 cut(s) | 458, 735, 1021, 1045, 1079 |
| BshFI | GGCC | 9 cut(s) | 128, 457, 871, 881, 917, 1020, 1078, 1823, 1980 |
| BshNI | GGYRCC | 2 cut(s) | 960, 1069 |
| BsiEI | CGRYCG | 5 cut(s) | 458, 735, 1021, 1045, 1079 |
| BsiHKAI | GWGCWC | 3 cut(s) | 701, 928, 1507 |
| BslFI | GGGAC | 5 cut(s) | 483, 562, 632, 965, 1456 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 297, 352, 586, 612, 747, 970, 991, 1411, 1831 |
| BsmAI | GTCTC | 6 cut(s) | 394, 702, 720, 1583, 1937, 2185 |
| BsmBI | CGTCTC | 5 cut(s) | 394, 702, 720, 1937, 2185 |
| BsmFI | GGGAC | 5 cut(s) | 483, 562, 632, 965, 1456 |
| BsnI | GGCC | 9 cut(s) | 128, 457, 871, 881, 917, 1020, 1078, 1823, 1980 |
| Bsp119I | TTCGAA | 1 cut(s) | 251 |
| Bsp1286I | GDGCHC | 4 cut(s) | 304, 701, 928, 1507 |
| Bsp19I | CCATGG | 2 cut(s) | 882, 1245 |
| BspANI | GGCC | 9 cut(s) | 128, 457, 871, 881, 917, 1020, 1078, 1823, 1980 |
| BspCNI | CTCAG | 5 cut(s) | 486, 615, 921, 1208, 2038 |
| BspFNI | CGCG | 9 cut(s) | 186, 199, 517, 551, 731, 815, 956, 1041, 1075 |
| BspHI | TCATGA | 3 cut(s) | 1221, 1527, 1951 |
| BspMAI | CTGCAG | 3 cut(s) | 84, 1564, 2166 |
| BspMI | ACCTGC | 2 cut(s) | 705, 1553 |
| BspPI | GGATC | 7 cut(s) | 253, 373, 403, 578, 935, 1005, 2114 |
| BspQI | GCTCTTC | 2 cut(s) | 309, 1495 |
| BspT104I | TTCGAA | 1 cut(s) | 251 |
| BspT107I | GGYRCC | 2 cut(s) | 960, 1069 |
| BsrBI | CCGCTC | 2 cut(s) | 174, 460 |
| BsrFI | RCCGGY | 1 cut(s) | 871 |
| BsrI | ACTGG | 7 cut(s) | 262, 975, 1174, 1208, 1382, 1445, 2042 |
| BssAI | RCCGGY | 1 cut(s) | 871 |
| BssECI | CCNNGG | 8 cut(s) | 123, 452, 882, 918, 944, 1039, 1073, 1245 |
| BssNI | GRCGYC | 5 cut(s) | 738, 786, 801, 1070, 1865 |
| BssSI | CACGAG | 2 cut(s) | 175, 478 |
| BssT1I | CCWWGG | 3 cut(s) | 882, 918, 1245 |
| Bst2BI | CACGAG | 2 cut(s) | 175, 478 |
| Bst2UI | CCWGG | 3 cut(s) | 125, 1397, 1658 |
| Bst4CI | ACNGT | 1 cut(s) | 1458 |
| Bst6I | CTCTTC | 6 cut(s) | 309, 344, 1495, 1531, 1797, 1877 |
| BstACI | GRCGYC | 5 cut(s) | 738, 786, 801, 1070, 1865 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 838 |
| BstBAI | YACGTR | 2 cut(s) | 559, 904 |
| BstBI | TTCGAA | 1 cut(s) | 251 |
| BstC8I | GCNNGC | 3 cut(s) | 488, 601, 1151 |
| BstDEI | CTNAG | 6 cut(s) | 473, 542, 623, 929, 1195, 2025 |
| BstDSI | CCRYGG | 4 cut(s) | 882, 1039, 1073, 1245 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1829 |
| BstF5I | GGATG | 3 cut(s) | 677, 1096, 1194 |
| BstFNI | CGCG | 9 cut(s) | 186, 199, 517, 551, 731, 815, 956, 1041, 1075 |
| BstH2I | RGCGCY | 1 cut(s) | 1073 |
| BstHHI | GCGC | 5 cut(s) | 186, 201, 530, 551, 1072 |
| BstMAI | GTCTC | 6 cut(s) | 394, 702, 720, 1583, 1937, 2185 |
| BstMCI | CGRYCG | 5 cut(s) | 458, 735, 1021, 1045, 1079 |
| BstNI | CCWGG | 3 cut(s) | 125, 1397, 1658 |
| BstNSI | RCATGY | 1 cut(s) | 841 |
| BstSFI | CTRYAG | 3 cut(s) | 80, 1560, 2162 |
| BstUI | CGCG | 9 cut(s) | 186, 199, 517, 551, 731, 815, 956, 1041, 1075 |
| BstV1I | GCAGC | 8 cut(s) | 66, 94, 97, 100, 495, 542, 1546, 1980 |
| BstV2I | GAAGAC | 1 cut(s) | 1443 |
| BstX2I | RGATCY | 1 cut(s) | 2119 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1925 |
| BstYI | RGATCY | 1 cut(s) | 2119 |
| BstZI | CGGCCG | 3 cut(s) | 455, 1018, 1076 |
| BsuI | GTATCC | 2 cut(s) | 1155, 2024 |
| BsuRI | GGCC | 9 cut(s) | 128, 457, 871, 881, 917, 1020, 1078, 1823, 1980 |
| BtgI | CCRYGG | 4 cut(s) | 882, 1039, 1073, 1245 |
| BtrI | CACGTC | 2 cut(s) | 895, 1063 |
| BtsCI | GGATG | 3 cut(s) | 677, 1096, 1194 |
| BtsI | GCAGTG | 2 cut(s) | 1634, 2171 |
| BtsIMutI | CAGTG | 5 cut(s) | 1181, 1201, 1634, 2049, 2171 |
| BveI | ACCTGC | 2 cut(s) | 705, 1553 |
| Cac8I | GCNNGC | 3 cut(s) | 488, 601, 1151 |
| CaiI | CAGNNNCTG | 1 cut(s) | 970 |
| CciI | TCATGA | 3 cut(s) | 1221, 1527, 1951 |
| CfoI | GCGC | 5 cut(s) | 186, 201, 530, 551, 1072 |
| Cfr10I | RCCGGY | 1 cut(s) | 871 |
| Cfr42I | CCGCGG | 2 cut(s) | 1042, 1076 |
| CseI | GACGC | 9 cut(s) | 175, 506, 737, 746, 790, 794, 821, 962, 1873 |
| CsiI | ACCWGGT | 1 cut(s) | 1656 |
| Csp6I | GTAC | 5 cut(s) | 642, 664, 901, 937, 2139 |
| CviQI | GTAC | 5 cut(s) | 642, 664, 901, 937, 2139 |
| DdeI | CTNAG | 6 cut(s) | 473, 542, 623, 929, 1195, 2025 |
| DinI | GGCGCC | 1 cut(s) | 1071 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 803 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1176 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 803 |
| EaeI | YGGCCR | 4 cut(s) | 455, 879, 1018, 1076 |
| EagI | CGGCCG | 3 cut(s) | 455, 1018, 1076 |
| Eam1104I | CTCTTC | 6 cut(s) | 309, 344, 1495, 1531, 1797, 1877 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1176 |
| EarI | CTCTTC | 6 cut(s) | 309, 344, 1495, 1531, 1797, 1877 |
| EciI | GGCGGA | 3 cut(s) | 323, 335, 1010 |
| Ecl136II | GAGCTC | 3 cut(s) | 699, 926, 1505 |
| EclXI | CGGCCG | 3 cut(s) | 455, 1018, 1076 |
| Eco130I | CCWWGG | 3 cut(s) | 882, 918, 1245 |
| Eco147I | AGGCCT | 1 cut(s) | 128 |
| Eco24I | GRGCYC | 4 cut(s) | 304, 701, 928, 1507 |
| Eco32I | GATATC | 1 cut(s) | 1878 |
| Eco47I | GGWCC | 8 cut(s) | 112, 322, 497, 576, 619, 1393, 1399, 1654 |
| Eco52I | CGGCCG | 3 cut(s) | 455, 1018, 1076 |
| Eco53kI | GAGCTC | 3 cut(s) | 699, 926, 1505 |
| Eco57I | CTGAAG | 2 cut(s) | 1724, 1725 |
| EcoICRI | GAGCTC | 3 cut(s) | 699, 926, 1505 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1829 |
| EcoO109I | RGGNCCY | 4 cut(s) | 576, 619, 1393, 1822 |
| EcoRI | GAATTC | 1 cut(s) | 253 |
| EcoRII | CCWGG | 3 cut(s) | 123, 1395, 1656 |
| EcoRV | GATATC | 1 cut(s) | 1878 |
| EcoT14I | CCWWGG | 3 cut(s) | 882, 918, 1245 |
| EcoT38I | GRGCYC | 4 cut(s) | 304, 701, 928, 1507 |
| EgeI | GGCGCC | 1 cut(s) | 1071 |
| EheI | GGCGCC | 1 cut(s) | 1071 |
| ErhI | CCWWGG | 3 cut(s) | 882, 918, 1245 |
| Esp3I | CGTCTC | 5 cut(s) | 394, 702, 720, 1937, 2185 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1310, 1342 |
| FaqI | GGGAC | 5 cut(s) | 483, 562, 632, 965, 1456 |
| FauI | CCCGC | 2 cut(s) | 333, 522 |
| FauNDI | CATATG | 1 cut(s) | 1780 |
| FblI | GTMKAC | 1 cut(s) | 807 |
| FokI | GGATG | 3 cut(s) | 684, 1103, 1201 |
| FriOI | GRGCYC | 4 cut(s) | 304, 701, 928, 1507 |
| FspBI | CTAG | 4 cut(s) | 140, 645, 1209, 1547 |
| FspI | TGCGCA | 1 cut(s) | 529 |
| GlaI | GCGC | 5 cut(s) | 185, 200, 529, 550, 1071 |
| GsuI | CTGGAG | 3 cut(s) | 769, 992, 2025 |
| HaeII | RGCGCY | 1 cut(s) | 1073 |
| HaeIII | GGCC | 9 cut(s) | 128, 457, 871, 881, 917, 1020, 1078, 1823, 1980 |
| HgaI | GACGC | 9 cut(s) | 175, 506, 737, 746, 790, 794, 821, 962, 1873 |
| HhaI | GCGC | 5 cut(s) | 186, 201, 530, 551, 1072 |
| Hin1I | GRCGYC | 5 cut(s) | 738, 786, 801, 1070, 1865 |
| Hin6I | GCGC | 5 cut(s) | 184, 199, 528, 549, 1070 |
| HinP1I | GCGC | 5 cut(s) | 184, 199, 528, 549, 1070 |
| HincII | GTYRAC | 1 cut(s) | 145 |
| HindII | GTYRAC | 1 cut(s) | 145 |
| HindIII | AAGCTT | 2 cut(s) | 1382, 1759 |
| HinfI | GANTC | 8 cut(s) | 43, 105, 974, 1126, 1576, 1648, 1769, 2023 |
| HphI | GGTGA | 5 cut(s) | 601, 781, 1677, 1757, 1847 |
| Hpy166II | GTNNAC | 9 cut(s) | 145, 556, 592, 772, 808, 864, 910, 1060, 1201 |
| Hpy188I | TCNGA | 8 cut(s) | 930, 979, 1002, 1047, 1308, 1362, 1704, 2206 |
| Hpy8I | GTNNAC | 9 cut(s) | 145, 556, 592, 772, 808, 864, 910, 1060, 1201 |
| Hpy99I | CGWCG | 8 cut(s) | 522, 611, 740, 746, 788, 806, 815, 1108 |
| HpyAV | CCTTC | 5 cut(s) | 814, 927, 1688, 1832, 2177 |
| HpyCH4III | ACNGT | 1 cut(s) | 1458 |
| HpyCH4IV | ACGT | 7 cut(s) | 31, 558, 594, 774, 894, 903, 1062 |
| HpyCH4V | TGCA | 7 cut(s) | 82, 765, 1497, 1562, 1968, 2060, 2164 |
| HpyF3I | CTNAG | 6 cut(s) | 473, 542, 623, 929, 1195, 2025 |
| HpySE526I | ACGT | 7 cut(s) | 31, 558, 594, 774, 894, 903, 1062 |
| Hsp92I | GRCGYC | 5 cut(s) | 738, 786, 801, 1070, 1865 |
| HspAI | GCGC | 5 cut(s) | 184, 199, 528, 549, 1070 |
| KasI | GGCGCC | 1 cut(s) | 1069 |
| KflI | GGGWCCC | 1 cut(s) | 576 |
| KspI | CCGCGG | 2 cut(s) | 1042, 1076 |
| LguI | GCTCTTC | 2 cut(s) | 309, 1495 |
| LmnI | GCTCC | 5 cut(s) | 608, 657, 704, 923, 2044 |
| Lsp1109I | GCAGC | 8 cut(s) | 66, 94, 97, 100, 495, 542, 1546, 1980 |
| LweI | GCATC | 2 cut(s) | 404, 1179 |
| MabI | ACCWGGT | 1 cut(s) | 1656 |
| MaeI | CTAG | 4 cut(s) | 140, 645, 1209, 1547 |
| MaeII | ACGT | 7 cut(s) | 31, 558, 594, 774, 894, 903, 1062 |
| MaeIII | GTNAC | 2 cut(s) | 1583, 1861 |
| MbiI | CCGCTC | 2 cut(s) | 174, 460 |
| MfeI | CAATTG | 1 cut(s) | 1476 |
| MflI | RGATCY | 1 cut(s) | 2119 |
| MhlI | GDGCHC | 4 cut(s) | 304, 701, 928, 1507 |
| MluI | ACGCGT | 1 cut(s) | 954 |
| Mly113I | GGCGCC | 1 cut(s) | 1070 |
| MlyI | GAGTC | 5 cut(s) | 52, 99, 983, 1585, 2017 |
| MmeI | TCCRAC | 1 cut(s) | 1025 |
| MseI | TTAA | 6 cut(s) | 1409, 1419, 1667, 1811, 2100, 2157 |
| MspA1I | CMGCKG | 6 cut(s) | 79, 486, 533, 1041, 1075, 1153 |
| MunI | CAATTG | 1 cut(s) | 1476 |
| MvaI | CCWGG | 3 cut(s) | 125, 1397, 1658 |
| MvnI | CGCG | 9 cut(s) | 186, 199, 517, 551, 731, 815, 956, 1041, 1075 |
| NarI | GGCGCC | 1 cut(s) | 1070 |
| NciI | CCSGG | 7 cut(s) | 102, 110, 281, 353, 454, 859, 945 |
| NcoI | CCATGG | 2 cut(s) | 882, 1245 |
| NdeI | CATATG | 1 cut(s) | 1780 |
| NmeAIII | GCCGAG | 2 cut(s) | 1104, 1164 |
| NmuCI | GTSAC | 1 cut(s) | 1861 |
| NsbI | TGCGCA | 1 cut(s) | 529 |
| NspI | RCATGY | 1 cut(s) | 841 |
| NspV | TTCGAA | 1 cut(s) | 251 |
| PagI | TCATGA | 3 cut(s) | 1221, 1527, 1951 |
| PaqCI | CACCTGC | 1 cut(s) | 1553 |
| PceI | AGGCCT | 1 cut(s) | 128 |
| PciSI | GCTCTTC | 2 cut(s) | 309, 1495 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 780, 900, 909 |
| PfeI | GAWTC | 3 cut(s) | 1126, 1648, 1769 |
| PflFI | GACNNNGTC | 2 cut(s) | 740, 800 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 970 |
| PfoI | TCCNGGA | 2 cut(s) | 108, 1395 |
| Ple19I | CGATCG | 1 cut(s) | 735 |
| PleI | GAGTC | 5 cut(s) | 51, 99, 982, 1584, 2017 |
| PluTI | GGCGCC | 1 cut(s) | 1073 |
| PpsI | GAGTC | 5 cut(s) | 51, 99, 982, 1584, 2017 |
| Ppu21I | YACGTR | 2 cut(s) | 559, 904 |
| PpuMI | RGGWCCY | 3 cut(s) | 576, 619, 1393 |
| Psp124BI | GAGCTC | 3 cut(s) | 701, 928, 1507 |
| Psp5II | RGGWCCY | 3 cut(s) | 576, 619, 1393 |
| Psp6I | CCWGG | 3 cut(s) | 123, 1395, 1656 |
| PspGI | CCWGG | 3 cut(s) | 123, 1395, 1656 |
| PspPPI | RGGWCCY | 3 cut(s) | 576, 619, 1393 |
| PstI | CTGCAG | 3 cut(s) | 84, 1564, 2166 |
| PstNI | CAGNNNCTG | 1 cut(s) | 970 |
| PsuI | RGATCY | 1 cut(s) | 2119 |
| PsyI | GACNNNGTC | 2 cut(s) | 740, 800 |
| PvuI | CGATCG | 1 cut(s) | 735 |
| PvuII | CAGCTG | 4 cut(s) | 79, 486, 533, 1153 |
| RsaI | GTAC | 5 cut(s) | 643, 665, 902, 938, 2140 |
| RsaNI | GTAC | 5 cut(s) | 642, 664, 901, 937, 2139 |
| SacI | GAGCTC | 3 cut(s) | 701, 928, 1507 |
| SacII | CCGCGG | 2 cut(s) | 1042, 1076 |
| SapI | GCTCTTC | 2 cut(s) | 309, 1495 |
| SaqAI | TTAA | 6 cut(s) | 1409, 1419, 1667, 1811, 2100, 2157 |
| ScaI | AGTACT | 2 cut(s) | 643, 665 |
| SchI | GAGTC | 5 cut(s) | 52, 99, 983, 1585, 2017 |
| SduI | GDGCHC | 4 cut(s) | 304, 701, 928, 1507 |
| SexAI | ACCWGGT | 1 cut(s) | 1656 |
| SfaNI | GCATC | 2 cut(s) | 404, 1179 |
| SfcI | CTRYAG | 3 cut(s) | 80, 1560, 2162 |
| SfoI | GGCGCC | 1 cut(s) | 1071 |
| Sfr303I | CCGCGG | 2 cut(s) | 1042, 1076 |
| SfuI | TTCGAA | 1 cut(s) | 251 |
| SgrBI | CCGCGG | 2 cut(s) | 1042, 1076 |
| SinI | GGWCC | 8 cut(s) | 112, 322, 497, 576, 619, 1393, 1399, 1654 |
| SmlI | CTYRAG | 1 cut(s) | 2018 |
| SmoI | CTYRAG | 1 cut(s) | 2018 |
| SseBI | AGGCCT | 1 cut(s) | 128 |
| SspDI | GGCGCC | 1 cut(s) | 1069 |
| SspI | AATATT | 1 cut(s) | 1735 |
| SspMI | CTAG | 4 cut(s) | 140, 645, 1209, 1547 |
| SstI | GAGCTC | 3 cut(s) | 701, 928, 1507 |
| StuI | AGGCCT | 1 cut(s) | 128 |
| StyI | CCWWGG | 3 cut(s) | 882, 918, 1245 |
| TaaI | ACNGT | 1 cut(s) | 1458 |
| TaiI | ACGT | 7 cut(s) | 34, 561, 597, 777, 897, 906, 1065 |
| TaqI | TCGA | 7 cut(s) | 251, 257, 609, 735, 1827, 1880, 1990 |
| TaqII | GACCGA | 1 cut(s) | 485 |
| TatI | WGTACW | 2 cut(s) | 641, 663 |
| TfiI | GAWTC | 3 cut(s) | 1126, 1648, 1769 |
| Tru1I | TTAA | 6 cut(s) | 1409, 1419, 1667, 1811, 2100, 2157 |
| Tru9I | TTAA | 6 cut(s) | 1409, 1419, 1667, 1811, 2100, 2157 |
| TscAI | CASTG | 5 cut(s) | 1181, 1208, 1641, 2049, 2171 |
| TseFI | GTSAC | 1 cut(s) | 1861 |
| TseI | GCWGC | 8 cut(s) | 79, 82, 85, 88, 483, 530, 1559, 1968 |
| Tsp45I | GTSAC | 1 cut(s) | 1861 |
| TspDTI | ATGAA | 9 cut(s) | 234, 349, 1210, 1238, 1242, 1364, 1670, 1940, 2118 |
| TspGWI | ACGGA | 4 cut(s) | 154, 376, 595, 1174 |
| TspRI | CASTG | 5 cut(s) | 1181, 1208, 1641, 2049, 2171 |
| Tth111I | GACNNNGTC | 2 cut(s) | 740, 800 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 970 |
| VpaK11BI | GGWCC | 8 cut(s) | 112, 322, 497, 576, 619, 1393, 1399, 1654 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1829 |
| XapI | RAATTY | 3 cut(s) | 253, 1669, 2125 |
| XceI | RCATGY | 1 cut(s) | 841 |
| XmiI | GTMKAC | 1 cut(s) | 807 |
| XspI | CTAG | 4 cut(s) | 140, 645, 1209, 1547 |
| ZrmI | AGTACT | 2 cut(s) | 643, 665 |