Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1331 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2494 |
| Acc36I | ACCTGC | 1 cut(s) | 2917 |
| Acc65I | GGTACC | 1 cut(s) | 631 |
| AccB1I | GGYRCC | 2 cut(s) | 631, 1454 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 1199, 1250, 1321 |
| AccBSI | CCGCTC | 1 cut(s) | 178 |
| AccI | GTMKAC | 1 cut(s) | 3188 |
| AciI | CCGC | 6 cut(s) | 178, 482, 771, 1204, 2411, 2733 |
| AclI | AACGTT | 1 cut(s) | 2615 |
| AclWI | GGATC | 9 cut(s) | 148, 1235, 1280, 1737, 1841, 2108, 2177, 2190, 3046 |
| AcoI | YGGCCR | 4 cut(s) | 408, 556, 721, 1907 |
| AcsI | RAATTY | 7 cut(s) | 731, 1031, 1178, 1246, 1325, 1814, 3260 |
| AcuI | CTGAAG | 4 cut(s) | 281, 2607, 2730, 3027 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2421 |
| AjnI | CCWGG | 3 cut(s) | 1009, 1100, 3034 |
| AjuI | GAANNNNNNNTTGG | 8 cut(s) | 360, 392, 1101, 1133, 1461, 1493, 2605, 2637 |
| Alw21I | GWGCWC | 3 cut(s) | 1191, 2866, 3289 |
| Alw26I | GTCTC | 6 cut(s) | 825, 2284, 2660, 2671, 2961, 3044 |
| Alw44I | GTGCAC | 1 cut(s) | 1187 |
| AlwI | GGATC | 9 cut(s) | 148, 1235, 1280, 1737, 1841, 2108, 2177, 2190, 3046 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1444, 3110 |
| Ama87I | CYCGRG | 4 cut(s) | 635, 1601, 1794, 1842 |
| AoxI | GGCC | 6 cut(s) | 273, 408, 556, 721, 870, 1907 |
| ApaLI | GTGCAC | 1 cut(s) | 1187 |
| ApeKI | GCWGC | 7 cut(s) | 1403, 1545, 1805, 2445, 2525, 3026, 3296 |
| ApoI | RAATTY | 7 cut(s) | 731, 1031, 1178, 1246, 1325, 1814, 3260 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1358, 1390, 3189, 3221 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 2151, 2758 |
| Asp718I | GGTACC | 1 cut(s) | 631 |
| AspLEI | GCGC | 4 cut(s) | 308, 565, 1314, 2275 |
| AspS9I | GGNCC | 2 cut(s) | 361, 3205 |
| AsuC2I | CCSGG | 2 cut(s) | 636, 637 |
| AsuHPI | GGTGA | 6 cut(s) | 2036, 2206, 2615, 2678, 3067, 3152 |
| AvaI | CYCGRG | 4 cut(s) | 635, 1601, 1794, 1842 |
| AvaII | GGWCC | 2 cut(s) | 361, 3205 |
| AxyI | CCTNAGG | 1 cut(s) | 348 |
| BaeGI | GKGCMC | 1 cut(s) | 1191 |
| BalI | TGGCCA | 3 cut(s) | 410, 558, 1909 |
| BamHI | GGATCC | 1 cut(s) | 2182 |
| BanI | GGYRCC | 2 cut(s) | 631, 1454 |
| BanII | GRGCYC | 2 cut(s) | 1884, 2635 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 504, 536, 749, 781 |
| BauI | CACGAG | 3 cut(s) | 822, 1407, 2960 |
| BbsI | GAAGAC | 3 cut(s) | 1031, 2144, 3231 |
| Bbv12I | GWGCWC | 3 cut(s) | 1191, 2866, 3289 |
| BbvI | GCAGC | 7 cut(s) | 1415, 1557, 1792, 2432, 2537, 3038, 3283 |
| BceAI | ACGGC | 5 cut(s) | 16, 708, 784, 809, 2351 |
| BciT130I | CCWGG | 3 cut(s) | 1011, 1102, 3036 |
| BciVI | GTATCC | 3 cut(s) | 577, 1117, 2249 |
| BcnI | CCSGG | 2 cut(s) | 636, 637 |
| BcoDI | GTCTC | 6 cut(s) | 825, 2284, 2660, 2671, 2961, 3044 |
| BfaI | CTAG | 8 cut(s) | 104, 708, 800, 1646, 1721, 1776, 2018, 2501 |
| BfmI | CTRYAG | 3 cut(s) | 1430, 1543, 3297 |
| BfoI | RGCGCY | 1 cut(s) | 1315 |
| BfuAI | ACCTGC | 1 cut(s) | 2917 |
| BfuI | GTATCC | 3 cut(s) | 577, 1117, 2249 |
| BisI | GCNGC | 7 cut(s) | 1404, 1546, 1806, 2446, 2526, 3027, 3297 |
| BlsI | GCNGC | 7 cut(s) | 1405, 1547, 1807, 2447, 2527, 3028, 3298 |
| Bme1390I | CCNGG | 5 cut(s) | 636, 637, 1011, 1102, 3036 |
| Bme18I | GGWCC | 2 cut(s) | 361, 3205 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2421 |
| BmeT110I | CYCGRG | 4 cut(s) | 635, 1601, 1794, 1842 |
| BmgT120I | GGNCC | 2 cut(s) | 361, 3205 |
| BmiI | GGNNCC | 9 cut(s) | 124, 363, 544, 633, 1456, 2031, 2184, 3094, 3207 |
| BmrFI | CCNGG | 5 cut(s) | 636, 637, 1011, 1102, 3036 |
| BpiI | GAAGAC | 3 cut(s) | 1031, 2144, 3231 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 795, 827 |
| BpmI | CTGGAG | 5 cut(s) | 55, 561, 1085, 2684, 2903 |
| BpuEI | CTTGAG | 3 cut(s) | 194, 1474, 1721 |
| BpuMI | CCSGG | 2 cut(s) | 636, 637 |
| BsaAI | YACGTR | 2 cut(s) | 1658, 2066 |
| BsaI | GGTCTC | 1 cut(s) | 825 |
| BsaJI | CCNNGG | 4 cut(s) | 432, 635, 636, 1101 |
| Bse1I | ACTGG | 9 cut(s) | 544, 763, 821, 1025, 1261, 1667, 1966, 2674, 2804 |
| Bse21I | CCTNAGG | 1 cut(s) | 348 |
| Bse3DI | GCAATG | 1 cut(s) | 2385 |
| BseBI | CCWGG | 3 cut(s) | 1011, 1102, 3036 |
| BseDI | CCNNGG | 4 cut(s) | 432, 635, 636, 1101 |
| BseGI | GGATG | 8 cut(s) | 334, 490, 1141, 1395, 1910, 2053, 2527, 2993 |
| BseMI | GCAATG | 1 cut(s) | 2385 |
| BseMII | CTCAG | 6 cut(s) | 339, 858, 1773, 1823, 2306, 2881 |
| BseNI | ACTGG | 9 cut(s) | 544, 763, 821, 1025, 1261, 1667, 1966, 2674, 2804 |
| BseRI | GAGGAG | 6 cut(s) | 179, 182, 363, 516, 1739, 2397 |
| BseSI | GKGCMC | 1 cut(s) | 1191 |
| BseXI | GCAGC | 7 cut(s) | 1415, 1557, 1792, 2432, 2537, 3038, 3283 |
| BshFI | GGCC | 6 cut(s) | 275, 410, 558, 723, 872, 1909 |
| BshNI | GGYRCC | 2 cut(s) | 631, 1454 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1191, 2866, 3289 |
| BsiHKCI | CYCGRG | 4 cut(s) | 635, 1601, 1794, 1842 |
| BsiSI | CCGG | 1 cut(s) | 636 |
| BslFI | GGGAC | 3 cut(s) | 278, 1704, 3191 |
| BsmAI | GTCTC | 6 cut(s) | 825, 2284, 2660, 2671, 2961, 3044 |
| BsmBI | CGTCTC | 2 cut(s) | 2284, 3044 |
| BsmFI | GGGAC | 3 cut(s) | 278, 1704, 3191 |
| BsnI | GGCC | 6 cut(s) | 275, 410, 558, 723, 872, 1909 |
| Bso31I | GGTCTC | 1 cut(s) | 825 |
| BsoBI | CYCGRG | 4 cut(s) | 635, 1601, 1794, 1842 |
| Bsp1286I | GDGCHC | 5 cut(s) | 1191, 1884, 2635, 2866, 3289 |
| Bsp1407I | TGTACA | 2 cut(s) | 1446, 2571 |
| BspACI | CCGC | 6 cut(s) | 178, 482, 771, 1204, 2411, 2733 |
| BspANI | GGCC | 6 cut(s) | 275, 410, 558, 723, 872, 1909 |
| BspCNI | CTCAG | 6 cut(s) | 340, 859, 1774, 1822, 2305, 2880 |
| BspHI | TCATGA | 1 cut(s) | 2766 |
| BspLI | GGNNCC | 9 cut(s) | 124, 363, 544, 633, 1456, 2031, 2184, 3094, 3207 |
| BspMAI | CTGCAG | 3 cut(s) | 1434, 1547, 3301 |
| BspMI | ACCTGC | 1 cut(s) | 2917 |
| BspPI | GGATC | 9 cut(s) | 148, 1235, 1280, 1737, 1841, 2108, 2177, 2190, 3046 |
| BspQI | GCTCTTC | 3 cut(s) | 16, 2268, 2706 |
| BspT107I | GGYRCC | 2 cut(s) | 631, 1454 |
| BspTNI | GGTCTC | 1 cut(s) | 825 |
| BsrBI | CCGCTC | 1 cut(s) | 178 |
| BsrDI | GCAATG | 1 cut(s) | 2385 |
| BsrGI | TGTACA | 2 cut(s) | 1446, 2571 |
| BsrI | ACTGG | 9 cut(s) | 544, 763, 821, 1025, 1261, 1667, 1966, 2674, 2804 |
| BssECI | CCNNGG | 4 cut(s) | 432, 635, 636, 1101 |
| BssSI | CACGAG | 3 cut(s) | 822, 1407, 2960 |
| Bst2BI | CACGAG | 3 cut(s) | 822, 1407, 2960 |
| Bst2UI | CCWGG | 3 cut(s) | 1011, 1102, 3036 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1712, 2645 |
| BstAUI | TGTACA | 2 cut(s) | 1446, 2571 |
| BstBAI | YACGTR | 2 cut(s) | 1658, 2066 |
| BstC8I | GCNNGC | 2 cut(s) | 252, 3243 |
| BstDSI | CCRYGG | 1 cut(s) | 432 |
| BstEII | GGTNACC | 2 cut(s) | 2333, 2904 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 161, 476 |
| BstF5I | GGATG | 8 cut(s) | 334, 490, 1141, 1395, 1910, 2053, 2527, 2993 |
| BstH2I | RGCGCY | 1 cut(s) | 1315 |
| BstHHI | GCGC | 4 cut(s) | 308, 565, 1314, 2275 |
| BstMAI | GTCTC | 6 cut(s) | 825, 2284, 2660, 2671, 2961, 3044 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 272, 656, 1318, 1412, 1712, 2645, 2870, 3293 |
| BstNI | CCWGG | 3 cut(s) | 1011, 1102, 3036 |
| BstNSI | RCATGY | 2 cut(s) | 2648, 3026 |
| BstPI | GGTNACC | 2 cut(s) | 2333, 2904 |
| BstSCI | CCNGG | 5 cut(s) | 634, 635, 1009, 1100, 3034 |
| BstSFI | CTRYAG | 3 cut(s) | 1430, 1543, 3297 |
| BstSLI | GKGCMC | 1 cut(s) | 1191 |
| BstV1I | GCAGC | 7 cut(s) | 1415, 1557, 1792, 2432, 2537, 3038, 3283 |
| BstV2I | GAAGAC | 3 cut(s) | 1031, 2144, 3231 |
| BstX2I | RGATCY | 4 cut(s) | 153, 1846, 2182, 3038 |
| BstYI | RGATCY | 4 cut(s) | 153, 1846, 2182, 3038 |
| Bsu36I | CCTNAGG | 1 cut(s) | 348 |
| BsuI | GTATCC | 3 cut(s) | 577, 1117, 2249 |
| BsuRI | GGCC | 6 cut(s) | 275, 410, 558, 723, 872, 1909 |
| BtgI | CCRYGG | 1 cut(s) | 432 |
| BtgZI | GCGATG | 1 cut(s) | 2409 |
| BtsCI | GGATG | 8 cut(s) | 334, 490, 1141, 1395, 1910, 2053, 2527, 2993 |
| BtsI | GCAGTG | 1 cut(s) | 2286 |
| BtsIMutI | CAGTG | 6 cut(s) | 864, 1581, 1762, 2253, 2286, 2496 |
| BveI | ACCTGC | 1 cut(s) | 2917 |
| Cac8I | GCNNGC | 2 cut(s) | 252, 3243 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1444, 3110 |
| CciI | TCATGA | 1 cut(s) | 2766 |
| CfoI | GCGC | 4 cut(s) | 308, 565, 1314, 2275 |
| Cfr13I | GGNCC | 2 cut(s) | 361, 3205 |
| Cfr9I | CCCGGG | 1 cut(s) | 635 |
| CseI | GACGC | 3 cut(s) | 97, 321, 2267 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2472, 2507 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2494 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2421 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2494 |
| EaeI | YGGCCR | 4 cut(s) | 408, 556, 721, 1907 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2421 |
| EciI | GGCGGA | 2 cut(s) | 497, 1219 |
| Eco147I | AGGCCT | 1 cut(s) | 872 |
| Eco24I | GRGCYC | 2 cut(s) | 1884, 2635 |
| Eco31I | GGTCTC | 1 cut(s) | 825 |
| Eco32I | GATATC | 1 cut(s) | 1972 |
| Eco47I | GGWCC | 2 cut(s) | 361, 3205 |
| Eco57I | CTGAAG | 4 cut(s) | 281, 2607, 2730, 3027 |
| Eco81I | CCTNAGG | 1 cut(s) | 348 |
| Eco88I | CYCGRG | 4 cut(s) | 635, 1601, 1794, 1842 |
| Eco91I | GGTNACC | 2 cut(s) | 2333, 2904 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 161, 476 |
| EcoO109I | RGGNCCY | 1 cut(s) | 3205 |
| EcoO65I | GGTNACC | 2 cut(s) | 2333, 2904 |
| EcoRI | GAATTC | 2 cut(s) | 731, 3260 |
| EcoRII | CCWGG | 3 cut(s) | 1009, 1100, 3034 |
| EcoRV | GATATC | 1 cut(s) | 1972 |
| EcoT22I | ATGCAT | 3 cut(s) | 2509, 2545, 2858 |
| EcoT38I | GRGCYC | 2 cut(s) | 1884, 2635 |
| Esp3I | CGTCTC | 2 cut(s) | 2284, 3044 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 382, 414, 882, 914, 2743, 2775, 3063, 3095 |
| FaqI | GGGAC | 3 cut(s) | 278, 1704, 3191 |
| FauI | CCCGC | 1 cut(s) | 2740 |
| FblI | GTMKAC | 1 cut(s) | 3188 |
| Fnu4HI | GCNGC | 7 cut(s) | 1404, 1546, 1806, 2446, 2526, 3027, 3297 |
| FokI | GGATG | 8 cut(s) | 341, 497, 1128, 1402, 1917, 2040, 2534, 2980 |
| FriOI | GRGCYC | 2 cut(s) | 1884, 2635 |
| Fsp4HI | GCNGC | 7 cut(s) | 1404, 1546, 1806, 2446, 2526, 3027, 3297 |
| FspBI | CTAG | 8 cut(s) | 104, 708, 800, 1646, 1721, 1776, 2018, 2501 |
| GlaI | GCGC | 4 cut(s) | 307, 564, 1313, 2274 |
| GluI | GCNGC | 7 cut(s) | 1404, 1546, 1806, 2446, 2526, 3027, 3297 |
| GsuI | CTGGAG | 5 cut(s) | 55, 561, 1085, 2684, 2903 |
| HaeII | RGCGCY | 1 cut(s) | 1315 |
| HaeIII | GGCC | 6 cut(s) | 275, 410, 558, 723, 872, 1909 |
| HapII | CCGG | 1 cut(s) | 636 |
| HgaI | GACGC | 3 cut(s) | 97, 321, 2267 |
| HhaI | GCGC | 4 cut(s) | 308, 565, 1314, 2275 |
| Hin6I | GCGC | 4 cut(s) | 306, 563, 1312, 2273 |
| HinP1I | GCGC | 4 cut(s) | 306, 563, 1312, 2273 |
| HincII | GTYRAC | 2 cut(s) | 643, 1540 |
| HindII | GTYRAC | 2 cut(s) | 643, 1540 |
| HindIII | AAGCTT | 3 cut(s) | 231, 2387, 3239 |
| HinfI | GANTC | 5 cut(s) | 97, 140, 343, 2416, 2455 |
| HpaI | GTTAAC | 1 cut(s) | 1540 |
| HpaII | CCGG | 1 cut(s) | 636 |
| HphI | GGTGA | 6 cut(s) | 2036, 2206, 2615, 2678, 3067, 3152 |
| Hpy99I | CGWCG | 1 cut(s) | 91 |
| HpyCH4IV | ACGT | 5 cut(s) | 1536, 1657, 2065, 2615, 2934 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 272, 656, 1318, 1412, 1712, 2645, 2870, 3293 |
| HpySE526I | ACGT | 5 cut(s) | 1536, 1657, 2065, 2615, 2934 |
| HspAI | GCGC | 4 cut(s) | 306, 563, 1312, 2273 |
| KpnI | GGTACC | 1 cut(s) | 635 |
| KspAI | GTTAAC | 1 cut(s) | 1540 |
| LguI | GCTCTTC | 3 cut(s) | 16, 2268, 2706 |
| LmnI | GCTCC | 5 cut(s) | 74, 169, 542, 803, 3092 |
| Lsp1109I | GCAGC | 7 cut(s) | 1415, 1557, 1792, 2432, 2537, 3038, 3283 |
| MaeI | CTAG | 8 cut(s) | 104, 708, 800, 1646, 1721, 1776, 2018, 2501 |
| MaeII | ACGT | 5 cut(s) | 1536, 1657, 2065, 2615, 2934 |
| MbiI | CCGCTC | 1 cut(s) | 178 |
| MfeI | CAATTG | 1 cut(s) | 576 |
| MflI | RGATCY | 4 cut(s) | 153, 1846, 2182, 3038 |
| MhlI | GDGCHC | 5 cut(s) | 1191, 1884, 2635, 2866, 3289 |
| MlsI | TGGCCA | 3 cut(s) | 410, 558, 1909 |
| MluNI | TGGCCA | 3 cut(s) | 410, 558, 1909 |
| MlyI | GAGTC | 1 cut(s) | 2410 |
| MmeI | TCCRAC | 6 cut(s) | 110, 117, 578, 1285, 1967, 2946 |
| Mox20I | TGGCCA | 3 cut(s) | 410, 558, 1909 |
| Mph1103I | ATGCAT | 3 cut(s) | 2509, 2545, 2858 |
| MroXI | GAANNNNTTC | 2 cut(s) | 2151, 2758 |
| MscI | TGGCCA | 3 cut(s) | 410, 558, 1909 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1146, 1170 |
| Msp20I | TGGCCA | 3 cut(s) | 410, 558, 1909 |
| MspA1I | CMGCKG | 1 cut(s) | 2445 |
| MspI | CCGG | 1 cut(s) | 636 |
| MspR9I | CCNGG | 5 cut(s) | 636, 637, 1011, 1102, 3036 |
| MunI | CAATTG | 1 cut(s) | 576 |
| MvaI | CCWGG | 3 cut(s) | 1011, 1102, 3036 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 272, 656, 1318, 1412, 1712, 2645, 2870, 3293 |
| NciI | CCSGG | 2 cut(s) | 636, 637 |
| NlaIV | GGNNCC | 9 cut(s) | 124, 363, 544, 633, 1456, 2031, 2184, 3094, 3207 |
| NmuCI | GTSAC | 8 cut(s) | 3, 535, 862, 1658, 1757, 2496, 2603, 2774 |
| NsiI | ATGCAT | 3 cut(s) | 2509, 2545, 2858 |
| NspI | RCATGY | 2 cut(s) | 2648, 3026 |
| PaeR7I | CTCGAG | 3 cut(s) | 1601, 1794, 1842 |
| PagI | TCATGA | 1 cut(s) | 2766 |
| PceI | AGGCCT | 1 cut(s) | 872 |
| PciSI | GCTCTTC | 3 cut(s) | 16, 2268, 2706 |
| PdmI | GAANNNNTTC | 2 cut(s) | 2151, 2758 |
| PfeI | GAWTC | 4 cut(s) | 97, 140, 343, 2455 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 1199, 1250, 1321 |
| PkrI | GCNGC | 7 cut(s) | 1405, 1547, 1807, 2447, 2527, 3028, 3298 |
| PleI | GAGTC | 1 cut(s) | 2410 |
| PpsI | GAGTC | 1 cut(s) | 2410 |
| Ppu21I | YACGTR | 2 cut(s) | 1658, 2066 |
| PpuMI | RGGWCCY | 1 cut(s) | 3205 |
| PsiI | TTATAA | 1 cut(s) | 1331 |
| Psp1406I | AACGTT | 1 cut(s) | 2615 |
| Psp5II | RGGWCCY | 1 cut(s) | 3205 |
| Psp6I | CCWGG | 3 cut(s) | 1009, 1100, 3034 |
| PspEI | GGTNACC | 2 cut(s) | 2333, 2904 |
| PspGI | CCWGG | 3 cut(s) | 1009, 1100, 3034 |
| PspN4I | GGNNCC | 9 cut(s) | 124, 363, 544, 633, 1456, 2031, 2184, 3094, 3207 |
| PspPI | GGNCC | 2 cut(s) | 361, 3205 |
| PspPPI | RGGWCCY | 1 cut(s) | 3205 |
| PspXI | VCTCGAGB | 1 cut(s) | 1601 |
| PstI | CTGCAG | 3 cut(s) | 1434, 1547, 3301 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1444, 3110 |
| PsuI | RGATCY | 4 cut(s) | 153, 1846, 2182, 3038 |
| PvuII | CAGCTG | 1 cut(s) | 2445 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1146, 1170 |
| SapI | GCTCTTC | 3 cut(s) | 16, 2268, 2706 |
| SatI | GCNGC | 7 cut(s) | 1404, 1546, 1806, 2446, 2526, 3027, 3297 |
| Sau96I | GGNCC | 2 cut(s) | 361, 3205 |
| SchI | GAGTC | 1 cut(s) | 2410 |
| ScrFI | CCNGG | 5 cut(s) | 636, 637, 1011, 1102, 3036 |
| SduI | GDGCHC | 5 cut(s) | 1191, 1884, 2635, 2866, 3289 |
| SfcI | CTRYAG | 3 cut(s) | 1430, 1543, 3297 |
| Sfr274I | CTCGAG | 3 cut(s) | 1601, 1794, 1842 |
| SinI | GGWCC | 2 cut(s) | 361, 3205 |
| SlaI | CTCGAG | 3 cut(s) | 1601, 1794, 1842 |
| SmaI | CCCGGG | 1 cut(s) | 637 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1146, 1170 |
| SmlI | CTYRAG | 6 cut(s) | 173, 1489, 1601, 1736, 1794, 1842 |
| SmoI | CTYRAG | 6 cut(s) | 173, 1489, 1601, 1736, 1794, 1842 |
| SseBI | AGGCCT | 1 cut(s) | 872 |
| SsiI | CCGC | 6 cut(s) | 178, 482, 771, 1204, 2411, 2733 |
| SspMI | CTAG | 8 cut(s) | 104, 708, 800, 1646, 1721, 1776, 2018, 2501 |
| StuI | AGGCCT | 1 cut(s) | 872 |
| StyD4I | CCNGG | 5 cut(s) | 634, 635, 1009, 1100, 3034 |
| TaiI | ACGT | 5 cut(s) | 1539, 1660, 2068, 2618, 2937 |
| TaqI | TCGA | 8 cut(s) | 675, 813, 978, 1602, 1795, 1843, 2323, 3264 |
| TatI | WGTACW | 4 cut(s) | 1446, 1620, 1855, 2571 |
| TfiI | GAWTC | 4 cut(s) | 97, 140, 343, 2455 |
| TscAI | CASTG | 6 cut(s) | 871, 1588, 1762, 2260, 2293, 2496 |
| TseFI | GTSAC | 8 cut(s) | 3, 535, 862, 1658, 1757, 2496, 2603, 2774 |
| TseI | GCWGC | 7 cut(s) | 1403, 1545, 1805, 2445, 2525, 3026, 3296 |
| Tsp45I | GTSAC | 8 cut(s) | 3, 535, 862, 1658, 1757, 2496, 2603, 2774 |
| TspDTI | ATGAA | 7 cut(s) | 1130, 1160, 1482, 1602, 2027, 2144, 2399 |
| TspGWI | ACGGA | 2 cut(s) | 2148, 3105 |
| TspMI | CCCGGG | 1 cut(s) | 635 |
| TspRI | CASTG | 6 cut(s) | 871, 1588, 1762, 2260, 2293, 2496 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 1199, 1250, 1321 |
| VneI | GTGCAC | 1 cut(s) | 1187 |
| VpaK11BI | GGWCC | 2 cut(s) | 361, 3205 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 161, 476 |
| XapI | RAATTY | 7 cut(s) | 731, 1031, 1178, 1246, 1325, 1814, 3260 |
| XbaI | TCTAGA | 1 cut(s) | 1720 |
| XceI | RCATGY | 2 cut(s) | 2648, 3026 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1102 |
| XhoI | CTCGAG | 3 cut(s) | 1601, 1794, 1842 |
| XmaI | CCCGGG | 1 cut(s) | 635 |
| XmiI | GTMKAC | 1 cut(s) | 3188 |
| XmnI | GAANNNNTTC | 2 cut(s) | 2151, 2758 |
| XspI | CTAG | 8 cut(s) | 104, 708, 800, 1646, 1721, 1776, 2018, 2501 |
| Zsp2I | ATGCAT | 3 cut(s) | 2509, 2545, 2858 |