Rmu_sc0000235.1_g000027

Villin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000235.1
OG0018355
Physical Location & Seq
Reverse (-)
71547 .. 72095
549 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
atgaatttcacaaattatgatctaaattttccagtcacactacattttatggccaaatacaagctcccctggatcttcaaatggcattacgaagtcaaagaacatgtagtctcaaggtatttcctagtcaaatggtgggataaattcaaaactgaaagaattatctcccaatttaatgaagaatttccagttactccatcacaacatactcagtcaagagctccagtcactccaatgccaccccaaacggcatccacttcccagtcacaacctaagtccctcaagtcacattcctcttccaaatccaaggggaaagcaaagtcaacaaaatcagatacatcctccaagtcagaagagttgaaagagttagcaaaaaggctcttagcacaagcagctgatctcagtaatgatgatgatgaatctgaagaagaagaagaaatttcttcccgatcctcatcatcatctcgcagtcatccaggcactcccggtaaaccagtcaaatgggctgatttccaagacagccaagacccatatgaacacttcgagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0006810 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010053 GO:0010054 GO:0010639 GO:0015629 GO:0016043 GO:0016049 GO:0016482 GO:0021700 GO:0022411 GO:0022622 GO:0030029 GO:0030036 GO:0030042 GO:0030154 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0033043 GO:0033993 GO:0040007 GO:0042221 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0043624 GO:0043933 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048523 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051261 GO:0051493 GO:0051494 GO:0051592 GO:0051641 GO:0051649 GO:0051693 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0080147 GO:0090066 GO:0090558 GO:0090627 GO:0097305 GO:0097435 GO:0099402 GO:0099636 GO:0110053 GO:1901700 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04666 ko04810 ko05203 map04666 map04810 map05203
Pfam Domains
Protein Families
Gelsolin VHP

Protein Analysis

182

Amino Acids

20.99

Weight (kDa)

6.67

Isoelectric Point (pI)

61.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 80, 444
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 5 cut(s) 4, 25, 143, 182, 438
AcuI CTGAAG 1 cut(s) 444
AflIII ACRYGT 1 cut(s) 103
AgsI TTSAA 3 cut(s) 79, 148, 361
AjnI CCWGG 2 cut(s) 68, 475
AluBI AGCT 3 cut(s) 64, 221, 395
AluI AGCT 3 cut(s) 64, 221, 395
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 115
AlwI GGATC 2 cut(s) 80, 444
AoxI GGCC 1 cut(s) 51
ApeKI GCWGC 1 cut(s) 392
ApoI RAATTY 5 cut(s) 4, 25, 143, 182, 438
Asp700I GAANNNNTTC 1 cut(s) 183
AsuC2I CCSGG 1 cut(s) 486
BalI TGGCCA 1 cut(s) 53
BanII GRGCYC 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 1 cut(s) 404
BccI CCATC 1 cut(s) 205
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 2 cut(s) 70, 477
BcnI CCSGG 1 cut(s) 486
BcoDI GTCTC 1 cut(s) 115
BfaI CTAG 1 cut(s) 125
BisI GCNGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 394
Bme1390I CCNGG 3 cut(s) 70, 477, 486
BmrFI CCNGG 3 cut(s) 70, 477, 486
BmrI ACTGGG 1 cut(s) 256
BmsI GCATC 1 cut(s) 260
BmuI ACTGGG 1 cut(s) 256
BpmI CTGGAG 1 cut(s) 207
BpuEI CTTGAG 2 cut(s) 97, 266
BpuMI CCSGG 1 cut(s) 486
BsaBI GATNNNNATC 1 cut(s) 454
BsaJI CCNNGG 2 cut(s) 68, 306
Bse1I ACTGG 5 cut(s) 32, 188, 224, 262, 494
Bse8I GATNNNNATC 1 cut(s) 454
BseBI CCWGG 2 cut(s) 70, 477
BseDI CCNNGG 2 cut(s) 68, 306
BseGI GGATG 3 cut(s) 251, 338, 472
BseJI GATNNNNATC 1 cut(s) 454
BseMII CTCAG 2 cut(s) 224, 415
BseNI ACTGG 5 cut(s) 32, 188, 224, 262, 494
BseXI GCAGC 1 cut(s) 404
BshFI GGCC 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 223
BsiSI CCGG 1 cut(s) 486
BslFI GGGAC 1 cut(s) 262
BsmAI GTCTC 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 4 cut(s) 19, 72, 397, 449
BspANI GGCC 1 cut(s) 53
BspCNI CTCAG 2 cut(s) 223, 414
BspPI GGATC 2 cut(s) 80, 444
BsrI ACTGG 5 cut(s) 32, 188, 224, 262, 494
BssECI CCNNGG 2 cut(s) 68, 306
BssMI GATC 4 cut(s) 19, 72, 397, 449
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 2 cut(s) 70, 477
Bst6I CTCTTC 2 cut(s) 301, 348
BstDEI CTNAG 4 cut(s) 210, 273, 382, 401
BstF5I GGATG 3 cut(s) 251, 338, 472
BstKTI GATC 4 cut(s) 22, 75, 400, 452
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 4 cut(s) 19, 72, 397, 449
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNI CCWGG 2 cut(s) 70, 477
BstNSI RCATGY 1 cut(s) 107
BstSCI CCNGG 3 cut(s) 68, 475, 484
BstV1I GCAGC 1 cut(s) 404
BstX2I RGATCY 1 cut(s) 72
BstXI CCANNNNNNTGG 1 cut(s) 501
BstYI RGATCY 1 cut(s) 72
BsuRI GGCC 1 cut(s) 53
BtsCI GGATG 3 cut(s) 251, 338, 472
CviAII CATG 1 cut(s) 104
CviJI RGCY 7 cut(s) 53, 64, 221, 379, 395, 506, 522
CviKI_1 RGCY 7 cut(s) 53, 64, 221, 379, 395, 506, 522
DdeI CTNAG 4 cut(s) 210, 273, 382, 401
DpnI GATC 4 cut(s) 21, 74, 399, 451
DpnII GATC 4 cut(s) 19, 72, 397, 449
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 2 cut(s) 301, 348
EarI CTCTTC 2 cut(s) 301, 348
Ecl136II GAGCTC 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 444
EcoICRI GAGCTC 1 cut(s) 221
EcoRII CCWGG 2 cut(s) 68, 475
EcoT14I CCWWGG 1 cut(s) 306
EcoT38I GRGCYC 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 1 cut(s) 107
FaiI YATR 6 cut(s) 18, 50, 105, 207, 532, 534
FaqI GGGAC 1 cut(s) 262
FatI CATG 1 cut(s) 103
FauNDI CATATG 1 cut(s) 532
Fnu4HI GCNGC 1 cut(s) 393
FokI GGATG 3 cut(s) 238, 325, 459
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 1 cut(s) 393
FspBI CTAG 1 cut(s) 125
GluI GCNGC 1 cut(s) 393
GsuI CTGGAG 1 cut(s) 207
HaeIII GGCC 1 cut(s) 53
HapII CCGG 1 cut(s) 486
Hin1II CATG 1 cut(s) 107
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HinfI GANTC 1 cut(s) 419
HpaII CCGG 1 cut(s) 486
Hpy166II GTNNAC 2 cut(s) 324, 491
Hpy188I TCNGA 3 cut(s) 334, 352, 424
Hpy188III TCNNGA 2 cut(s) 216, 447
Hpy8I GTNNAC 2 cut(s) 324, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
HpyF3I CTNAG 4 cut(s) 210, 273, 382, 401
Hsp92II CATG 1 cut(s) 107
Kzo9I GATC 4 cut(s) 19, 72, 397, 449
LmnI GCTCC 2 cut(s) 69, 226
Lsp1109I GCAGC 1 cut(s) 404
LweI GCATC 1 cut(s) 260
MaeI CTAG 1 cut(s) 125
MaeIII GTNAC 5 cut(s) 34, 190, 226, 264, 285
MalI GATC 4 cut(s) 21, 74, 399, 451
MboI GATC 4 cut(s) 19, 72, 397, 449
MboII GAAGA 9 cut(s) 67, 191, 288, 365, 435, 437, 440, 443, 446
MflI RGATCY 1 cut(s) 72
MhlI GDGCHC 1 cut(s) 223
MlsI TGGCCA 1 cut(s) 53
MluCI AATT 8 cut(s) 4, 13, 25, 143, 159, 170, 182, 438
MluNI TGGCCA 1 cut(s) 53
MnlI CCTC 4 cut(s) 290, 304, 352, 463
Mox20I TGGCCA 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 183
MscI TGGCCA 1 cut(s) 53
MseI TTAA 1 cut(s) 174
MslI CAYNNNNRTG 1 cut(s) 233
Msp20I TGGCCA 1 cut(s) 53
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 1 cut(s) 486
MspR9I CCNGG 3 cut(s) 70, 477, 486
MvaI CCWGG 2 cut(s) 70, 477
MwoI GCNNNNNNNGC 1 cut(s) 392
NciI CCSGG 1 cut(s) 486
NdeI CATATG 1 cut(s) 532
NdeII GATC 4 cut(s) 19, 72, 397, 449
NlaIII CATG 1 cut(s) 107
NmuCI GTSAC 4 cut(s) 34, 226, 264, 285
NspI RCATGY 1 cut(s) 107
PciI ACATGT 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 1 cut(s) 419
PkrI GCNGC 1 cut(s) 394
PscI ACATGT 1 cut(s) 103
Psp124BI GAGCTC 1 cut(s) 223
Psp6I CCWGG 2 cut(s) 68, 475
PspGI CCWGG 2 cut(s) 68, 475
PsuI RGATCY 1 cut(s) 72
PvuII CAGCTG 1 cut(s) 395
RseI CAYNNNNRTG 1 cut(s) 233
SacI GAGCTC 1 cut(s) 223
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 1 cut(s) 393
Sau3AI GATC 4 cut(s) 19, 72, 397, 449
ScrFI CCNGG 3 cut(s) 70, 477, 486
SduI GDGCHC 1 cut(s) 223
SetI ASST 5 cut(s) 66, 119, 223, 274, 397
SfaNI GCATC 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 233
SmlI CTYRAG 2 cut(s) 112, 281
SmoI CTYRAG 2 cut(s) 112, 281
Sse9I AATT 8 cut(s) 4, 13, 25, 143, 159, 170, 182, 438
SspMI CTAG 1 cut(s) 125
SstI GAGCTC 1 cut(s) 223
StyD4I CCNGG 3 cut(s) 68, 475, 484
StyI CCWWGG 1 cut(s) 306
TaqI TCGA 1 cut(s) 543
TasI AATT 8 cut(s) 4, 13, 25, 143, 159, 170, 182, 438
TfiI GAWTC 1 cut(s) 419
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TseFI GTSAC 4 cut(s) 34, 226, 264, 285
TseI GCWGC 1 cut(s) 392
Tsp45I GTSAC 4 cut(s) 34, 226, 264, 285
TspDTI ATGAA 4 cut(s) 17, 192, 432, 549
XapI RAATTY 5 cut(s) 4, 25, 143, 182, 438
XceI RCATGY 1 cut(s) 107
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.