Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000332.1

Orthogroup ID

OG0012855

Physical Location & Seq

Genomic Coordinates Reverse (-)

191375 .. 194905

Gene Structure 3531 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3087 bp

atgcggctcctccttctcctcctcctcctcctccacctccgccactctctctcctcaccgcaccgtctcctctccacagccacagccacacgcccaatgtcgacctaccgggccctcctctccctcaaggcctccatcaccgacgaccccgactcccgcctctcctcctggacccccaacaccagccactgcacctgggcaggcgtcacgtgcgactcccgccgtcacgtgacctccctcgacctttccggcttgaatctctccggctatctctcccccgatatcgcccaccttgtctacctctctaaccttactctcgccgacaacacgttctcgggtcctatcccgcccgagatctcagccctctcggggctccgactcctcaacctctccaacaacgtcttcaaccgcacgttcccgccggagctctccaacctcacaaacctccgcgtgctcgacctctacaacaacaacttgaccggggaattgccggtatcagtgacccagatgaccaacctcaggcacttgcatttgggtggcaactttttcgagggttcgatcccgccggagtacggccggttcccgtttctcgagtacttggccgtctccggcaactctatcaccggcaaaatccccaaggagattggaaacctgaccaggctcaaggagctttacattggctactacaacagctacgacggcggcataccggccgagatcgggaacttgacagaactcgtccggttcgacgccgccaactgcaatttatccggcgagatcccgccggaactgggtcggttgcaaaatgtggatactctgtttctccaggtgaatgcgctttccgggtcattgacgtctgagctgggctacctcaagagcttgaagtccatggacttatccaacaacttgttctccggcgagattccggtctctttcgcagagctaaagaacctgacgcttctcaatctcttccggaaccagcttcacggcgccattcccgacttcatcggtgacttgccggagctccaggttctgcagctgtgggagaacaacttcactgggtctattcctcagggtttgggcagaaatgggaagcttcaaatcctcgacttgtcttcgaacaaattgacaggagctcttcctcctgatatgtgcacagggaacaatcttcaaaccctcatcactcttggcaactttctcttcggtcccattccagagtcactcggagactgccagtcactcagtcggattcgaatgggtgagaactttctaaatgggtcgattcctaaaggtctatttgggttgcccaaactcacccaagtggagctgcaagacaatttcttaggcggttcgtttccagagtctgattcaatctctggtgcgctcggtcagattagtctttccaacaatcggttatccgggcccttgccgccgaccatcggaaacttctccggcgtccagaagcttctccttgacgggaacaagttctcgggtcgaatcccgccggagattggacgcctgcagcaactgtcaaagattgattttagccacaacatgttttcgggtccaattgcgccggcgatcagccaatgcaagctgctgacgtttgttgatctcagtcggaatgatctctccggtgagattccgaaagagattaccggtatgaggatactgaattacctcaatctctcgaggaaccatctcgtggggaacatcccatcttccatttcctcaatgcaaagcttaacctcggtggatttttcgtataacaatctttcgggtttggttccggggactggtcagttcagctacttcaattacacttcgtttttgggcaactctgatctttgtggtccctatttggttccttgcaaagatggagttgcaaatggcactcatcagcctcgcgtcaaaagctcattcactgcttcattgaagctattgcttgttgtcgggttgctactctgctccatcatatttgcagtgattacaataatcaaagctcgatcgtacaagagagagagggattccagagcttggaagttgactgcattccagcgcttggatttcaccgttgatgatgtcttggattcactgaaggaagacaacattataggcaaaggaggtgctgggattgtgtacaaaggcgccatgcctagtggtgacaatgtggctgtgaaaagactccctgcaatgagcagaggctcatctcatgatcatggattcaatgccgagattcagactctgggtcgaattcgacaccgtcacattgttagattgttgggtttctgttcaaaccatgagacaaatcttctggtatacgagtacatgcctaatgggagtttgggtgaggttcttcatggcaagaaaggaggtcacttgcattgggatacaaggtacaagattgctattgaagctgcaaaggggctttgctatcttcaccatgactgttcgcctctgatagtccatagagatgtgaaatcgaataacattctcctcgactccaattttgaggctcatgttgctgattttggccttgccaagttccttcaggattccggcacgtccgagtgcatgtctgccattgctggttcttatggatacatcgctccagagtatgcctacacactgaaggtcgatgagaagagcgacgtctatagttttggtgtagttctcttagaacttgttagtggcaagaaaccagttggtgaatttggagatggggtcgatatagtccaatgggttcggaaaatgacggattcaaacaaggaaggagtcctgaagatccttgattccagactcccgtctgttcccctacatgaggttatgcacgtattttacgtcgcaatgctctgcgttgaagaacaagcagtggaacgtcccacgatgagagaagtggttcaaattcttacagagcttccaattccaccaggctcaaagcaaggaggagacacaacaacaatcacagactcccctcaacaatctgccattattactgcaatggaatcaccaactaccacaaaagacacgaaagaccatcaacagccaccgccacaatcgccaccgccggatcttctaagcatttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009908 GO:0010073 GO:0010075 GO:0016020 GO:0022414 GO:0032501 GO:0032502 GO:0033612 GO:0040008 GO:0043621 GO:0044464 GO:0048229 GO:0048367 GO:0048437 GO:0048507 GO:0048509 GO:0048608 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0061458 GO:0065007 GO:0071944 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1028

Amino Acids

112.4

Weight (kDa)

6.27

Isoelectric Point (pI)

36.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	37 - 72	3.9e-12	Leucine rich repeat N-terminal domain
LRR_14	PF23598	118 - 179	5e-07	Leucine-rich repeat region
LRR_14	PF23598	141 - 278	9.3e-14	Leucine-rich repeat region
LRR_4	PF12799	147 - 181	1.9e-06	Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr	PF07714	701 - 970	1.7e-45	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	701 - 968	2.4e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0012855)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49670 AT5G65700 AT5G65700
fragaria_vesca	FvH4_1g18710
malus_domestica	MD02G1194600.v1.1 MD15G1304900.v1.1
prunus_persica	Prupe.6G212200_v2.0.a1
pyrus_communis	pycom02g15850 pycom15g26730
rosa_chinensis	RchiOBHm_Chr2g0109581
rosa_laevigata	RLG00000017747
rosa_multiflora	Rmu_sc0000332.1_g000045
rosa_roxburghii	Rroxscaffold_2G00134130
rosa_rugosa	Rorug02G0160800
rosa_samantha	Rh2AG213200 Rh2BG223400 Rh2CG215200 Rh2DG218700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	857, 2655
AccB1I	GGYRCC	2 cut(s)	989, 2147
AccB7I	CCANNNNNTGG	3 cut(s)	1705, 2037, 2062
AccI	GTMKAC	3 cut(s)	101, 297, 2319
AccII	CGCG	2 cut(s)	450, 1908
AccIII	TCCGGA	1 cut(s)	972
AclWI	GGATC	4 cut(s)	553, 772, 2779, 3078
AcoI	YGGCCR	3 cut(s)	574, 600, 711
AcsI	RAATTY	3 cut(s)	2253, 2711, 2904
AcuI	CTGAAG	4 cut(s)	2117, 2534, 2651, 2801
AcvI	CACGTG	2 cut(s)	210, 229
AcyI	GRCGYC	8 cut(s)	204, 750, 854, 990, 1455, 1516, 2148, 2652
AfaI	GTAC	6 cut(s)	572, 596, 2012, 2141, 2327, 2399
AfeI	AGCGCT	1 cut(s)	2060
AflIII	ACRYGT	2 cut(s)	327, 1554
AgeI	ACCGGT	1 cut(s)	1658
AhdI	GACNNNNNGTC	2 cut(s)	1234, 2247
AjiI	CACGTC	1 cut(s)	2565
AjnI	CCWGG	6 cut(s)	167, 194, 656, 825, 1026, 2929
AjuI	GAANNNNNNNTTGG	4 cut(s)	386, 418, 893, 925
AleI	CACNNNNGTG	1 cut(s)	1319
Alw21I	GWGCWC	5 cut(s)	429, 456, 1026, 1138, 1157
Alw26I	GTCTC	6 cut(s)	71, 610, 934, 1221, 2297, 2943
Alw44I	GTGCAC	1 cut(s)	1153
AlwI	GGATC	4 cut(s)	553, 772, 2779, 3078
AlwNI	CAGNNNCTG	5 cut(s)	189, 1033, 1364, 1528, 2245
Ama87I	CYCGRG	6 cut(s)	334, 350, 367, 590, 1489, 1690
Aor13HI	TCCGGA	1 cut(s)	972
Aor51HI	AGCGCT	1 cut(s)	2060
AoxI	GGCC	7 cut(s)	111, 129, 574, 600, 711, 1421, 2533
ApaI	GGGCCC	2 cut(s)	115, 1425
ApaLI	GTGCAC	1 cut(s)	1153
ApeKI	GCWGC	5 cut(s)	1036, 1327, 1522, 1597, 2417
ApoI	RAATTY	3 cut(s)	2253, 2711, 2904
ArsI	GACNNNNNNTTYG	2 cut(s)	2492, 2524
AsiGI	ACCGGT	1 cut(s)	1658
Asp700I	GAANNNNTTC	3 cut(s)	1052, 1484, 2898
AspLEI	GCGC	6 cut(s)	838, 992, 1384, 1576, 2061, 2150
AspS9I	GGNCC	9 cut(s)	111, 112, 171, 338, 1205, 1421, 1422, 1565, 1853
AsuC2I	CCSGG	5 cut(s)	110, 481, 844, 1420, 1791
AsuII	TTCGAA	2 cut(s)	1118, 1252
AvaI	CYCGRG	6 cut(s)	334, 350, 367, 590, 1489, 1690
AvaII	GGWCC	5 cut(s)	171, 338, 1205, 1565, 1853
AxyI	CCTNAGG	2 cut(s)	518, 1071
BaeGI	GKGCMC	3 cut(s)	115, 1157, 1425
BanI	GGYRCC	2 cut(s)	989, 2147
BanII	GRGCYC	6 cut(s)	115, 375, 429, 1026, 1138, 1425
BauI	CACGAG	1 cut(s)	1703
BbrPI	CACGTG	2 cut(s)	210, 229
BbsI	GAAGAC	3 cut(s)	394, 1107, 2109
Bbv12I	GWGCWC	5 cut(s)	429, 456, 1026, 1138, 1157
BbvI	GCAGC	5 cut(s)	1048, 1314, 1534, 1584, 2404
BccI	CCATC	8 cut(s)	143, 1445, 1707, 1726, 1871, 1979, 2714, 3045
BceAI	ACGGC	5 cut(s)	207, 587, 589, 715, 1003
BcgI	CGANNNNNNTGC	2 cut(s)	529, 563
BciT130I	CCWGG	6 cut(s)	169, 196, 658, 827, 1028, 2931
BciVI	GTATCC	4 cut(s)	805, 1662, 2383, 2594
BclI	TGATCA	1 cut(s)	2215
BcnI	CCSGG	5 cut(s)	110, 481, 844, 1420, 1791
BcoDI	GTCTC	6 cut(s)	71, 610, 934, 1221, 2297, 2943
BfaI	CTAG	1 cut(s)	2157
BfmI	CTRYAG	3 cut(s)	1034, 1520, 2656
BfoI	RGCGCY	3 cut(s)	993, 2062, 2151
BfuI	GTATCC	4 cut(s)	805, 1662, 2383, 2594
BglII	AGATCT	1 cut(s)	354
BisI	GCNGC	9 cut(s)	5, 703, 753, 1037, 1328, 1430, 1523, 1598, 2418
BlsI	GCNGC	9 cut(s)	6, 704, 754, 1038, 1329, 1431, 1524, 1599, 2419
BmcAI	AGTACT	1 cut(s)	596
Bme18I	GGWCC	5 cut(s)	171, 338, 1205, 1565, 1853
BmeRI	GACNNNNNGTC	2 cut(s)	1234, 2247
BmeT110I	CYCGRG	6 cut(s)	334, 350, 367, 590, 1489, 1690
BmgBI	CACGTC	1 cut(s)	2565
BmgT120I	GGNCC	9 cut(s)	111, 112, 171, 338, 1205, 1421, 1422, 1565, 1853
BmrI	ACTGGG	2 cut(s)	800, 1068
BmuI	ACTGGG	2 cut(s)	800, 1068
BoxI	GACNNNNGTC	1 cut(s)	2803
BpiI	GAAGAC	3 cut(s)	394, 1107, 2109
BpmI	CTGGAG	3 cut(s)	809, 1010, 2595
Bpu14I	TTCGAA	2 cut(s)	1118, 1252
BpuEI	CTTGAG	3 cut(s)	110, 647, 857
BpuMI	CCSGG	5 cut(s)	110, 481, 844, 1420, 1791
BsaAI	YACGTR	3 cut(s)	210, 229, 2833
BsaHI	GRCGYC	8 cut(s)	204, 750, 854, 990, 1455, 1516, 2148, 2652
BsaI	GGTCTC	1 cut(s)	934
BsaJI	CCNNGG	6 cut(s)	195, 480, 636, 888, 1749, 1790
BsaWI	WCCGGW	5 cut(s)	741, 925, 972, 1634, 1658
Bse118I	RCCGGY	6 cut(s)	490, 576, 623, 709, 1576, 1658
Bse1I	ACTGG	5 cut(s)	795, 1063, 1234, 1801, 2702
Bse21I	CCTNAGG	2 cut(s)	518, 1071
Bse3DI	GCAATG	4 cut(s)	2199, 2583, 2853, 3006
BseAI	TCCGGA	1 cut(s)	972
BseBI	CCWGG	6 cut(s)	169, 196, 658, 827, 1028, 2931
BseDI	CCNNGG	6 cut(s)	195, 480, 636, 888, 1749, 1790
BseGI	GGATG	1 cut(s)	1713
BseMI	GCAATG	4 cut(s)	2199, 2583, 2853, 3006
BseMII	CTCAG	6 cut(s)	372, 532, 849, 1085, 1255, 1630
BseNI	ACTGG	5 cut(s)	795, 1063, 1234, 1801, 2702
BseSI	GKGCMC	3 cut(s)	115, 1157, 1425
BseX3I	CGGCCG	2 cut(s)	574, 711
BseXI	GCAGC	5 cut(s)	1048, 1314, 1534, 1584, 2404
BseYI	CCCAGC	2 cut(s)	862, 2129
BsgI	GTGCAG	1 cut(s)	175
Bsh1236I	CGCG	2 cut(s)	450, 1908
Bsh1285I	CGRYCG	3 cut(s)	577, 714, 2009
BshFI	GGCC	7 cut(s)	113, 131, 576, 602, 713, 1423, 2535
BshNI	GGYRCC	2 cut(s)	989, 2147
BshTI	ACCGGT	1 cut(s)	1658
BsiEI	CGRYCG	3 cut(s)	577, 714, 2009
BsiHKAI	GWGCWC	5 cut(s)	429, 456, 1026, 1138, 1157
BsiHKCI	CYCGRG	6 cut(s)	334, 350, 367, 590, 1489, 1690
BslFI	GGGAC	4 cut(s)	1191, 1807, 1839, 2865
BsmAI	GTCTC	6 cut(s)	71, 610, 934, 1221, 2297, 2943
BsmBI	CGTCTC	2 cut(s)	71, 610
BsmFI	GGGAC	4 cut(s)	1191, 1807, 1839, 2865
BsmI	GAATGC	2 cut(s)	838, 2051
BsnI	GGCC	7 cut(s)	113, 131, 576, 602, 713, 1423, 2535
Bso31I	GGTCTC	1 cut(s)	934
BsoBI	CYCGRG	6 cut(s)	334, 350, 367, 590, 1489, 1690
Bsp119I	TTCGAA	2 cut(s)	1118, 1252
Bsp120I	GGGCCC	2 cut(s)	111, 1421
Bsp1286I	GDGCHC	8 cut(s)	115, 375, 429, 456, 1026, 1138, 1157, 1425
Bsp13I	TCCGGA	1 cut(s)	972
Bsp1407I	TGTACA	1 cut(s)	2139
Bsp19I	CCATGG	1 cut(s)	888
BspANI	GGCC	7 cut(s)	113, 131, 576, 602, 713, 1423, 2535
BspCNI	CTCAG	6 cut(s)	371, 531, 850, 1084, 1254, 1629
BspEI	TCCGGA	1 cut(s)	972
BspFNI	CGCG	2 cut(s)	450, 1908
BspHI	TCATGA	1 cut(s)	2212
BspMAI	CTGCAG	2 cut(s)	1038, 1524
BspPI	GGATC	4 cut(s)	553, 772, 2779, 3078
BspQI	GCTCTTC	2 cut(s)	1143, 2639
BspT104I	TTCGAA	2 cut(s)	1118, 1252
BspT107I	GGYRCC	2 cut(s)	989, 2147
BspTNI	GGTCTC	1 cut(s)	934
BsrDI	GCAATG	4 cut(s)	2199, 2583, 2853, 3006
BsrFI	RCCGGY	6 cut(s)	490, 576, 623, 709, 1576, 1658
BsrGI	TGTACA	1 cut(s)	2139
BsrI	ACTGG	5 cut(s)	795, 1063, 1234, 1801, 2702
BssAI	RCCGGY	6 cut(s)	490, 576, 623, 709, 1576, 1658
BssECI	CCNNGG	6 cut(s)	195, 480, 636, 888, 1749, 1790
BssNAI	GTATAC	1 cut(s)	2320
BssNI	GRCGYC	8 cut(s)	204, 750, 854, 990, 1455, 1516, 2148, 2652
BssSI	CACGAG	1 cut(s)	1703
BssT1I	CCWWGG	2 cut(s)	636, 888
Bst1107I	GTATAC	1 cut(s)	2320
Bst2BI	CACGAG	1 cut(s)	1703
Bst2UI	CCWGG	6 cut(s)	169, 196, 658, 827, 1028, 2931
Bst4CI	ACNGT	5 cut(s)	65, 1530, 2074, 2264, 2450
Bst6I	CTCTTC	4 cut(s)	974, 1143, 1205, 2639
BstACI	GRCGYC	8 cut(s)	204, 750, 854, 990, 1455, 1516, 2148, 2652
BstAUI	TGTACA	1 cut(s)	2139
BstBAI	YACGTR	3 cut(s)	210, 229, 2833
BstBI	TTCGAA	2 cut(s)	1118, 1252
BstC8I	GCNNGC	5 cut(s)	202, 452, 1520, 1578, 1595
BstDEI	CTNAG	9 cut(s)	358, 518, 858, 1071, 1241, 1342, 1616, 2677, 3077
BstDSI	CCRYGG	1 cut(s)	888
BstENI	CCTNNNNNAGG	1 cut(s)	2819
BstF5I	GGATG	1 cut(s)	1713
BstFNI	CGCG	2 cut(s)	450, 1908
BstH2I	RGCGCY	3 cut(s)	993, 2062, 2151
BstHHI	GCGC	6 cut(s)	838, 992, 1384, 1576, 2061, 2150
BstMAI	GTCTC	6 cut(s)	71, 610, 934, 1221, 2297, 2943
BstMCI	CGRYCG	3 cut(s)	577, 714, 2009
BstMWI	GCNNNNNNNGC	9 cut(s)	210, 219, 667, 699, 1429, 1914, 2414, 2522, 3058
BstNI	CCWGG	6 cut(s)	169, 196, 658, 827, 1028, 2931
BstNSI	RCATGY	3 cut(s)	1558, 2332, 2578
BstPAI	GACNNNNGTC	1 cut(s)	2803
BstSFI	CTRYAG	3 cut(s)	1034, 1520, 2656
BstSLI	GKGCMC	3 cut(s)	115, 1157, 1425
BstUI	CGCG	2 cut(s)	450, 1908
BstV1I	GCAGC	5 cut(s)	1048, 1314, 1534, 1584, 2404
BstV2I	GAAGAC	3 cut(s)	394, 1107, 2109
BstX2I	RGATCY	4 cut(s)	354, 777, 2784, 3070
BstYI	RGATCY	4 cut(s)	354, 777, 2784, 3070
BstZ17I	GTATAC	1 cut(s)	2320
BstZI	CGGCCG	2 cut(s)	574, 711
Bsu36I	CCTNAGG	2 cut(s)	518, 1071
BsuI	GTATCC	4 cut(s)	805, 1662, 2383, 2594
BsuRI	GGCC	7 cut(s)	113, 131, 576, 602, 713, 1423, 2535
BtgI	CCRYGG	1 cut(s)	888
BtgZI	GCGATG	1 cut(s)	2590
BtrI	CACGTC	1 cut(s)	2565
BtsCI	GGATG	1 cut(s)	1713
BtsI	GCAGTG	4 cut(s)	187, 1923, 1989, 2877
BtsIMutI	CAGTG	8 cut(s)	187, 504, 1056, 1923, 1989, 2093, 2627, 2877
Cac8I	GCNNGC	5 cut(s)	202, 452, 1520, 1578, 1595
CaiI	CAGNNNCTG	5 cut(s)	189, 1033, 1364, 1528, 2245
CciI	TCATGA	1 cut(s)	2212
CfoI	GCGC	6 cut(s)	838, 992, 1384, 1576, 2061, 2150
Cfr10I	RCCGGY	6 cut(s)	490, 576, 623, 709, 1576, 1658
Cfr13I	GGNCC	9 cut(s)	111, 112, 171, 338, 1205, 1421, 1422, 1565, 1853
CseI	GACGC	6 cut(s)	193, 758, 964, 1444, 1524, 1897
Csp6I	GTAC	6 cut(s)	571, 595, 2011, 2140, 2326, 2398
CspAI	ACCGGT	1 cut(s)	1658
CviQI	GTAC	6 cut(s)	571, 595, 2011, 2140, 2326, 2398
DdeI	CTNAG	9 cut(s)	358, 518, 858, 1071, 1241, 1342, 1616, 2677, 3077
DinI	GGCGCC	2 cut(s)	991, 2149
DriI	GACNNNNNGTC	2 cut(s)	1234, 2247
EaeI	YGGCCR	3 cut(s)	574, 600, 711
EagI	CGGCCG	2 cut(s)	574, 711
Eam1104I	CTCTTC	4 cut(s)	974, 1143, 1205, 2639
Eam1105I	GACNNNNNGTC	2 cut(s)	1234, 2247
EarI	CTCTTC	4 cut(s)	974, 1143, 1205, 2639
EciI	GGCGGA	1 cut(s)	29
Ecl136II	GAGCTC	3 cut(s)	427, 1024, 1136
EclXI	CGGCCG	2 cut(s)	574, 711
Eco130I	CCWWGG	2 cut(s)	636, 888
Eco147I	AGGCCT	1 cut(s)	131
Eco24I	GRGCYC	6 cut(s)	115, 375, 429, 1026, 1138, 1425
Eco31I	GGTCTC	1 cut(s)	934
Eco32I	GATATC	1 cut(s)	283
Eco47I	GGWCC	5 cut(s)	171, 338, 1205, 1565, 1853
Eco47III	AGCGCT	1 cut(s)	2060
Eco52I	CGGCCG	2 cut(s)	574, 711
Eco53kI	GAGCTC	3 cut(s)	427, 1024, 1136
Eco57I	CTGAAG	4 cut(s)	2117, 2534, 2651, 2801
Eco72I	CACGTG	2 cut(s)	210, 229
Eco81I	CCTNAGG	2 cut(s)	518, 1071
Eco88I	CYCGRG	6 cut(s)	334, 350, 367, 590, 1489, 1690
EcoICRI	GAGCTC	3 cut(s)	427, 1024, 1136
EcoNI	CCTNNNNNAGG	1 cut(s)	2819
EcoO109I	RGGNCCY	3 cut(s)	112, 338, 1422
EcoRI	GAATTC	1 cut(s)	2253
EcoRII	CCWGG	6 cut(s)	167, 194, 656, 825, 1026, 2929
EcoRV	GATATC	1 cut(s)	283
EcoT14I	CCWWGG	2 cut(s)	636, 888
EcoT38I	GRGCYC	6 cut(s)	115, 375, 429, 1026, 1138, 1425
EgeI	GGCGCC	2 cut(s)	991, 2149
EheI	GGCGCC	2 cut(s)	991, 2149
ErhI	CCWWGG	2 cut(s)	636, 888
Esp3I	CGTCTC	2 cut(s)	71, 610
FaqI	GGGAC	4 cut(s)	1191, 1807, 1839, 2865
FauI	CCCGC	7 cut(s)	164, 227, 354, 426, 570, 789, 1509
FbaI	TGATCA	1 cut(s)	2215
FblI	GTMKAC	3 cut(s)	101, 297, 2319
Fnu4HI	GCNGC	9 cut(s)	5, 703, 753, 1037, 1328, 1430, 1523, 1598, 2418
FokI	GGATG	1 cut(s)	1700
FriOI	GRGCYC	6 cut(s)	115, 375, 429, 1026, 1138, 1425
Fsp4HI	GCNGC	9 cut(s)	5, 703, 753, 1037, 1328, 1430, 1523, 1598, 2418
FspBI	CTAG	1 cut(s)	2157
GlaI	GCGC	6 cut(s)	837, 991, 1383, 1575, 2060, 2149
GluI	GCNGC	9 cut(s)	5, 703, 753, 1037, 1328, 1430, 1523, 1598, 2418
GsaI	CCCAGC	2 cut(s)	866, 2133
GsuI	CTGGAG	3 cut(s)	809, 1010, 2595
HaeII	RGCGCY	3 cut(s)	993, 2062, 2151
HaeIII	GGCC	7 cut(s)	113, 131, 576, 602, 713, 1423, 2535
HgaI	GACGC	6 cut(s)	193, 758, 964, 1444, 1524, 1897
HhaI	GCGC	6 cut(s)	838, 992, 1384, 1576, 2061, 2150
Hin1I	GRCGYC	8 cut(s)	204, 750, 854, 990, 1455, 1516, 2148, 2652
Hin6I	GCGC	6 cut(s)	836, 990, 1382, 1574, 2059, 2148
HinP1I	GCGC	6 cut(s)	836, 990, 1382, 1574, 2059, 2148
HincII	GTYRAC	2 cut(s)	102, 2046
HindII	GTYRAC	2 cut(s)	102, 2046
HindIII	AAGCTT	3 cut(s)	1094, 1463, 1741
Hpy166II	GTNNAC	6 cut(s)	102, 298, 1155, 2046, 2140, 2320
Hpy8I	GTNNAC	6 cut(s)	102, 298, 1155, 2046, 2140, 2320
Hpy99I	CGWCG	5 cut(s)	146, 701, 752, 2654, 2846
HpyAV	CCTTC	5 cut(s)	23, 2092, 2558, 2626, 2765
HpyCH4III	ACNGT	5 cut(s)	65, 1530, 2074, 2264, 2450
HpyF10VI	GCNNNNNNNGC	9 cut(s)	210, 219, 667, 699, 1429, 1914, 2414, 2522, 3058
HpyF3I	CTNAG	9 cut(s)	358, 518, 858, 1071, 1241, 1342, 1616, 2677, 3077
Hsp92I	GRCGYC	8 cut(s)	204, 750, 854, 990, 1455, 1516, 2148, 2652
HspAI	GCGC	6 cut(s)	836, 990, 1382, 1574, 2059, 2148
KasI	GGCGCC	2 cut(s)	989, 2147
Kpn2I	TCCGGA	1 cut(s)	972
KroI	GCCGGC	1 cut(s)	1576
KroNI	GCCGGC	1 cut(s)	1578
Ksp22I	TGATCA	1 cut(s)	2215
LguI	GCTCTTC	2 cut(s)	1143, 2639
Lsp1109I	GCAGC	5 cut(s)	1048, 1314, 1534, 1584, 2404
MaeI	CTAG	1 cut(s)	2157
MfeI	CAATTG	1 cut(s)	1569
MflI	RGATCY	4 cut(s)	354, 777, 2784, 3070
MhlI	GDGCHC	8 cut(s)	115, 375, 429, 456, 1026, 1138, 1157, 1425
Mly113I	GGCGCC	2 cut(s)	990, 2148
MmeI	TCCRAC	7 cut(s)	400, 417, 456, 924, 1226, 1428, 1601
MreI	CGCCGGCG	1 cut(s)	1576
MroI	TCCGGA	1 cut(s)	972
MroNI	GCCGGC	1 cut(s)	1576
MroXI	GAANNNNTTC	3 cut(s)	1052, 1484, 2898
MseI	TTAA	1 cut(s)	1745
MslI	CAYNNNNRTG	4 cut(s)	534, 1319, 2217, 2472
MspA1I	CMGCKG	1 cut(s)	1039
MunI	CAATTG	1 cut(s)	1569
Mva1269I	GAATGC	2 cut(s)	838, 2051
MvaI	CCWGG	6 cut(s)	169, 196, 658, 827, 1028, 2931
MvnI	CGCG	2 cut(s)	450, 1908
MwoI	GCNNNNNNNGC	9 cut(s)	210, 219, 667, 699, 1429, 1914, 2414, 2522, 3058
NaeI	GCCGGC	1 cut(s)	1578
NarI	GGCGCC	2 cut(s)	990, 2148
NciI	CCSGG	5 cut(s)	110, 481, 844, 1420, 1791
NcoI	CCATGG	1 cut(s)	888
NgoMIV	GCCGGC	1 cut(s)	1576
NmeAIII	GCCGAG	2 cut(s)	739, 2257
NspI	RCATGY	3 cut(s)	1558, 2332, 2578
NspV	TTCGAA	2 cut(s)	1118, 1252
OliI	CACNNNNGTG	1 cut(s)	1319
PaeR7I	CTCGAG	2 cut(s)	590, 1690
PagI	TCATGA	1 cut(s)	2212
PceI	AGGCCT	1 cut(s)	131
PciI	ACATGT	1 cut(s)	1554
PciSI	GCTCTTC	2 cut(s)	1143, 2639
PcsI	WCGNNNNNNNCGW	2 cut(s)	744, 2838
PctI	GAATGC	2 cut(s)	838, 2051
PdiI	GCCGGC	1 cut(s)	1578
PdmI	GAANNNNTTC	3 cut(s)	1052, 1484, 2898
PflFI	GACNNNGTC	1 cut(s)	2262
PflMI	CCANNNNNTGG	3 cut(s)	1705, 2037, 2062
PfoI	TCCNGGA	1 cut(s)	167
PinAI	ACCGGT	1 cut(s)	1658
PkrI	GCNGC	9 cut(s)	6, 704, 754, 1038, 1329, 1431, 1524, 1599, 2419
Ple19I	CGATCG	1 cut(s)	2009
PluTI	GGCGCC	2 cut(s)	993, 2151
PmaCI	CACGTG	2 cut(s)	210, 229
PmlI	CACGTG	2 cut(s)	210, 229
Ppu21I	YACGTR	3 cut(s)	210, 229, 2833
PpuMI	RGGWCCY	1 cut(s)	338
PscI	ACATGT	1 cut(s)	1554
PshAI	GACNNNNGTC	1 cut(s)	2803
Psp124BI	GAGCTC	3 cut(s)	429, 1026, 1138
Psp5II	RGGWCCY	1 cut(s)	338
Psp6I	CCWGG	6 cut(s)	167, 194, 656, 825, 1026, 2929
PspCI	CACGTG	2 cut(s)	210, 229
PspFI	CCCAGC	2 cut(s)	862, 2129
PspGI	CCWGG	6 cut(s)	167, 194, 656, 825, 1026, 2929
PspOMI	GGGCCC	2 cut(s)	111, 1421
PspPI	GGNCC	9 cut(s)	111, 112, 171, 338, 1205, 1421, 1422, 1565, 1853
PspPPI	RGGWCCY	1 cut(s)	338
PsrI	GAACNNNNNNTAC	2 cut(s)	563, 595
PstI	CTGCAG	2 cut(s)	1038, 1524
PstNI	CAGNNNCTG	5 cut(s)	189, 1033, 1364, 1528, 2245
PsuI	RGATCY	4 cut(s)	354, 777, 2784, 3070
PsyI	GACNNNGTC	1 cut(s)	2262
PvuI	CGATCG	1 cut(s)	2009
PvuII	CAGCTG	1 cut(s)	1039
RsaI	GTAC	6 cut(s)	572, 596, 2012, 2141, 2327, 2399
RsaNI	GTAC	6 cut(s)	571, 595, 2011, 2140, 2326, 2398
RseI	CAYNNNNRTG	4 cut(s)	534, 1319, 2217, 2472
SacI	GAGCTC	3 cut(s)	429, 1026, 1138
SalI	GTCGAC	1 cut(s)	100
SapI	GCTCTTC	2 cut(s)	1143, 2639
SaqAI	TTAA	1 cut(s)	1745
SatI	GCNGC	9 cut(s)	5, 703, 753, 1037, 1328, 1430, 1523, 1598, 2418
Sau96I	GGNCC	9 cut(s)	111, 112, 171, 338, 1205, 1421, 1422, 1565, 1853
ScaI	AGTACT	1 cut(s)	596
SduI	GDGCHC	8 cut(s)	115, 375, 429, 456, 1026, 1138, 1157, 1425
SfcI	CTRYAG	3 cut(s)	1034, 1520, 2656
SfoI	GGCGCC	2 cut(s)	991, 2149
Sfr274I	CTCGAG	2 cut(s)	590, 1690
SfuI	TTCGAA	2 cut(s)	1118, 1252
SgrAI	CRCCGGYG	1 cut(s)	1576
SinI	GGWCC	5 cut(s)	171, 338, 1205, 1565, 1853
SlaI	CTCGAG	2 cut(s)	590, 1690
SmiMI	CAYNNNNRTG	4 cut(s)	534, 1319, 2217, 2472
SmlI	CTYRAG	5 cut(s)	125, 590, 662, 872, 1690
SmoI	CTYRAG	5 cut(s)	125, 590, 662, 872, 1690
SseBI	AGGCCT	1 cut(s)	131
SspDI	GGCGCC	2 cut(s)	989, 2147
SspMI	CTAG	1 cut(s)	2157
SstI	GAGCTC	3 cut(s)	429, 1026, 1138
StuI	AGGCCT	1 cut(s)	131
StyI	CCWWGG	2 cut(s)	636, 888
TaaI	ACNGT	5 cut(s)	65, 1530, 2074, 2264, 2450
TaqII	GACCGA	2 cut(s)	1193, 1376
TatI	WGTACW	3 cut(s)	594, 2139, 2325
TauI	GCSGC	4 cut(s)	7, 705, 755, 1432
Tru1I	TTAA	1 cut(s)	1745
Tru9I	TTAA	1 cut(s)	1745
TscAI	CASTG	8 cut(s)	194, 504, 1063, 1930, 1989, 2100, 2634, 2877
TseI	GCWGC	5 cut(s)	1036, 1327, 1522, 1597, 2417
TspDTI	ATGAA	3 cut(s)	994, 1920, 2348
TspGWI	ACGGA	1 cut(s)	2771
TspRI	CASTG	8 cut(s)	194, 504, 1063, 1930, 1989, 2100, 2634, 2877
Tth111I	GACNNNGTC	1 cut(s)	2262
Van91I	CCANNNNNTGG	3 cut(s)	1705, 2037, 2062
VneI	GTGCAC	1 cut(s)	1153
VpaK11BI	GGWCC	5 cut(s)	171, 338, 1205, 1565, 1853
XagI	CCTNNNNNAGG	1 cut(s)	2819
XapI	RAATTY	3 cut(s)	2253, 2711, 2904
XceI	RCATGY	3 cut(s)	1558, 2332, 2578
XhoI	CTCGAG	2 cut(s)	590, 1690
XmiI	GTMKAC	3 cut(s)	101, 297, 2319
XmnI	GAANNNNTTC	3 cut(s)	1052, 1484, 2898
XspI	CTAG	1 cut(s)	2157
ZraI	GACGTC	2 cut(s)	855, 2653
ZrmI	AGTACT	1 cut(s)	596

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.