Rmu_sc0000470.1_g000018

maintenance of mitotic sister chromatid cohesion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000470.1
OG0007251
Physical Location & Seq
Reverse (-)
81100 .. 83924
2825 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1080 bp
atggatgcggtggcggaagggctgtgggggttggcggactaccaggagcagagcggcgagatcggcaaagccgtcaagtgcctggaagccatctgccaaagcgacgtgtcgtttttccccatcgtcgaggtcaagactcgtctccgaatcgctactcttctcctcaagcactcccacaacgtcaaccacgccaaggctcacttggagcgcgcgcagttgctgctcaaatccattccttcctgttttgatttgaagtgcagagcttacagtctgctcagccagtgttaccatctcgtcggctccatagctcctcagaaacacgtcctccacaaggccctggagctcactgctagcggctacgatattgcagtgaagttatggtcatgcaacttcaattcgcagctggcaaatgcgttgattattgagggagactaccagagttcgattgcggcattggacgccggctatgtttgtgctactcagataggttatcctgagttgcagatgttctttgcaacttgtatgcttcatgtacatctcatgcactgggaggatgagagttcagttgagcgagctgctgcgaaatgcgatgaggtctgggagttccttgacccccagaaatatttgacaatccttgggagtttggcactggcgctgcatgacgctggacaagcaagagagattttgagatcatccttgacattggcaaagaagctttctgatatccccacgcagatatgggtgctctctgttttgacagctttatatcaagaattaggtgagaagggaagtgaaatggagaatgttgagtttcagaagagtaggatggatgctctgcaacagaaacttatagatgcaaattcatccattcaccatattgaactaattgacacagtaaggattgaagtccagcaatttcatgaggttgacagtcgttcaacccttggcccagccatgagcgccaatcttgacattccagaatctgttggcctgtctgccccaatgcccggtcagtcaacatcaaggctagtagatatagacataggaaacataggaagacgtgggaagaggaaaatgtag

Protein Analysis

359

Amino Acids

39.81

Weight (kDa)

5.78

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cohesin_load PF10345 22 - 197 3e-16 Cohesin loading factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 54
AccII CGCG 2 cut(s) 210, 212
AciI CCGC 6 cut(s) 8, 14, 35, 54, 354, 449
AcsI RAATTY 1 cut(s) 859
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 1 cut(s) 534
AfiI CCNNNNNNNGG 3 cut(s) 193, 331, 1007
AflIII ACRYGT 2 cut(s) 105, 319
AgsI TTSAA 5 cut(s) 253, 394, 881, 905, 939
AjiI CACGTC 3 cut(s) 106, 322, 1061
AjnI CCWGG 3 cut(s) 42, 81, 336
AluBI AGCT 7 cut(s) 263, 308, 343, 403, 575, 715, 761
AluI AGCT 7 cut(s) 263, 308, 343, 403, 575, 715, 761
Alw21I GWGCWC 2 cut(s) 345, 747
Alw26I GTCTC 2 cut(s) 146, 423
AlwNI CAGNNNCTG 2 cut(s) 220, 983
AoxI GGCC 3 cut(s) 333, 946, 988
ApeKI GCWGC 5 cut(s) 220, 400, 575, 578, 655
ApoI RAATTY 1 cut(s) 859
AspLEI GCGC 5 cut(s) 210, 212, 214, 655, 962
AspS9I GGNCC 2 cut(s) 334, 947
AsuC2I CCSGG 1 cut(s) 1008
AsuHPI GGTGA 2 cut(s) 791, 863
AsuNHI GCTAGC 1 cut(s) 350
BanII GRGCYC 1 cut(s) 345
BbsI GAAGAC 1 cut(s) 1063
Bbv12I GWGCWC 2 cut(s) 345, 747
BbvI GCAGC 5 cut(s) 207, 412, 562, 565, 642
BccI CCATC 4 cut(s) 98, 128, 297, 820
BceAI ACGGC 1 cut(s) 56
BciT130I CCWGG 3 cut(s) 44, 83, 338
BcnI CCSGG 1 cut(s) 1008
BcoDI GTCTC 2 cut(s) 146, 423
BfaI CTAG 2 cut(s) 351, 1028
BfoI RGCGCY 2 cut(s) 656, 963
BisI GCNGC 8 cut(s) 55, 221, 355, 401, 450, 576, 579, 656
BlpI GCTNAGC 1 cut(s) 275
BlsI GCNGC 8 cut(s) 56, 222, 356, 402, 451, 577, 580, 657
Bme1390I CCNGG 4 cut(s) 44, 83, 338, 1008
BmgBI CACGTC 3 cut(s) 106, 322, 1061
BmgT120I GGNCC 2 cut(s) 334, 947
BmiI GGNNCC 1 cut(s) 301
BmrFI CCNGG 4 cut(s) 44, 83, 338, 1008
BmrI ACTGGG 1 cut(s) 556
BmsI GCATC 2 cut(s) 820, 844
BmtI GCTAGC 1 cut(s) 354
BmuI ACTGGG 1 cut(s) 556
BpiI GAAGAC 1 cut(s) 1063
BpmI CTGGAG 1 cut(s) 359
Bpu1102I GCTNAGC 1 cut(s) 275
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 1008
BsaHI GRCGYC 1 cut(s) 459
BsaJI CCNNGG 4 cut(s) 192, 336, 634, 943
BsaXI ACNNNNNCTCC 2 cut(s) 309, 339
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 331, 1007
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 3 cut(s) 280, 551, 654
BseBI CCWGG 3 cut(s) 44, 83, 338
BseDI CCNNGG 4 cut(s) 192, 336, 634, 943
BseGI GGATG 6 cut(s) 10, 559, 692, 831, 835, 863
BseLI CCNNNNNNNGG 3 cut(s) 193, 331, 1007
BseMII CTCAG 4 cut(s) 289, 326, 486, 494
BseNI ACTGG 3 cut(s) 280, 551, 654
BsePI GCGCGC 2 cut(s) 208, 210
BseRI GAGGAG 2 cut(s) 152, 300
BseXI GCAGC 5 cut(s) 207, 412, 562, 565, 642
BseYI CCCAGC 1 cut(s) 949
BsgI GTGCAG 1 cut(s) 277
Bsh1236I CGCG 2 cut(s) 210, 212
BshFI GGCC 3 cut(s) 335, 948, 990
BsiHKAI GWGCWC 2 cut(s) 345, 747
BsiSI CCGG 2 cut(s) 462, 1008
BslI CCNNNNNNNGG 3 cut(s) 193, 331, 1007
BsmAI GTCTC 2 cut(s) 146, 423
BsmBI CGTCTC 1 cut(s) 146
BsnI GGCC 3 cut(s) 335, 948, 990
Bsp1286I GDGCHC 2 cut(s) 345, 747
Bsp1407I TGTACA 1 cut(s) 532
Bsp143I GATC 2 cut(s) 60, 689
Bsp1720I GCTNAGC 1 cut(s) 275
BspACI CCGC 6 cut(s) 8, 14, 35, 54, 354, 449
BspANI GGCC 3 cut(s) 335, 948, 990
BspCNI CTCAG 4 cut(s) 288, 325, 487, 493
BspFNI CGCG 2 cut(s) 210, 212
BspHI TCATGA 1 cut(s) 919
BspLI GGNNCC 1 cut(s) 301
BspOI GCTAGC 1 cut(s) 354
BsrBI CCGCTC 1 cut(s) 54
BsrFI RCCGGY 1 cut(s) 461
BsrGI TGTACA 1 cut(s) 532
BsrI ACTGG 3 cut(s) 280, 551, 654
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 4 cut(s) 192, 336, 634, 943
BssHII GCGCGC 2 cut(s) 208, 210
BssMI GATC 2 cut(s) 60, 689
BssNI GRCGYC 1 cut(s) 459
BssT1I CCWWGG 3 cut(s) 192, 634, 943
Bst2UI CCWGG 3 cut(s) 44, 83, 338
Bst4CI ACNGT 3 cut(s) 269, 895, 932
Bst6I CTCTTC 3 cut(s) 162, 812, 1061
BstACI GRCGYC 1 cut(s) 459
BstAPI GCANNNNNTGC 1 cut(s) 220
BstAUI TGTACA 1 cut(s) 532
BstC8I GCNNGC 6 cut(s) 210, 212, 352, 405, 463, 573
BstDEI CTNAG 4 cut(s) 275, 312, 480, 495
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 6 cut(s) 10, 559, 692, 831, 835, 863
BstFNI CGCG 2 cut(s) 210, 212
BstH2I RGCGCY 2 cut(s) 656, 963
BstHHI GCGC 5 cut(s) 210, 212, 214, 655, 962
BstKTI GATC 2 cut(s) 63, 692
BstMAI GTCTC 2 cut(s) 146, 423
BstMBI GATC 2 cut(s) 60, 689
BstMWI GCNNNNNNNGC 5 cut(s) 63, 220, 458, 671, 959
BstNI CCWGG 3 cut(s) 44, 83, 338
BstSCI CCNGG 4 cut(s) 42, 81, 336, 1006
BstUI CGCG 2 cut(s) 210, 212
BstV1I GCAGC 5 cut(s) 207, 412, 562, 565, 642
BstV2I GAAGAC 1 cut(s) 1063
BsuRI GGCC 3 cut(s) 335, 948, 990
BtgZI GCGATG 1 cut(s) 603
BtrI CACGTC 3 cut(s) 106, 322, 1061
BtsCI GGATG 6 cut(s) 10, 559, 692, 831, 835, 863
BtsI GCAGTG 2 cut(s) 345, 375
BtsIMutI CAGTG 5 cut(s) 287, 345, 375, 544, 647
Cac8I GCNNGC 6 cut(s) 210, 212, 352, 405, 463, 573
CaiI CAGNNNCTG 2 cut(s) 220, 983
CciI TCATGA 1 cut(s) 919
CfoI GCGC 5 cut(s) 210, 212, 214, 655, 962
Cfr10I RCCGGY 1 cut(s) 461
Cfr13I GGNCC 2 cut(s) 334, 947
CseI GACGC 2 cut(s) 467, 671
Csp6I GTAC 1 cut(s) 533
CviAII CATG 6 cut(s) 384, 530, 541, 659, 920, 955
CviQI GTAC 1 cut(s) 533
DdeI CTNAG 4 cut(s) 275, 312, 480, 495
DpnI GATC 2 cut(s) 62, 691
DpnII GATC 2 cut(s) 60, 689
Eam1104I CTCTTC 3 cut(s) 162, 812, 1061
EarI CTCTTC 3 cut(s) 162, 812, 1061
EciI GGCGGA 2 cut(s) 29, 50
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 3 cut(s) 192, 634, 943
Eco24I GRGCYC 1 cut(s) 345
Eco32I GATATC 1 cut(s) 724
Eco53kI GAGCTC 1 cut(s) 343
EcoICRI GAGCTC 1 cut(s) 343
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 334
EcoRII CCWGG 3 cut(s) 42, 81, 336
EcoRV GATATC 1 cut(s) 724
EcoT14I CCWWGG 3 cut(s) 192, 634, 943
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 3 cut(s) 192, 634, 943
Esp3I CGTCTC 1 cut(s) 146
FaeI CATG 6 cut(s) 387, 533, 544, 662, 923, 958
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FatI CATG 6 cut(s) 383, 529, 540, 658, 919, 954
Fnu4HI GCNGC 8 cut(s) 55, 221, 355, 401, 450, 576, 579, 656
FokI GGATG 6 cut(s) 17, 566, 679, 838, 842, 850
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 8 cut(s) 55, 221, 355, 401, 450, 576, 579, 656
FspBI CTAG 2 cut(s) 351, 1028
GlaI GCGC 5 cut(s) 209, 211, 213, 654, 961
GluI GCNGC 8 cut(s) 55, 221, 355, 401, 450, 576, 579, 656
GsaI CCCAGC 1 cut(s) 953
GsuI CTGGAG 1 cut(s) 359
HaeII RGCGCY 2 cut(s) 656, 963
HaeIII GGCC 3 cut(s) 335, 948, 990
HapII CCGG 2 cut(s) 462, 1008
HgaI GACGC 2 cut(s) 467, 671
HhaI GCGC 5 cut(s) 210, 212, 214, 655, 962
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 6 cut(s) 387, 533, 544, 662, 923, 958
Hin6I GCGC 5 cut(s) 208, 210, 212, 653, 960
HinP1I GCGC 5 cut(s) 208, 210, 212, 653, 960
HincII GTYRAC 3 cut(s) 184, 928, 1017
HindII GTYRAC 3 cut(s) 184, 928, 1017
HindIII AAGCTT 1 cut(s) 713
HinfI GANTC 3 cut(s) 136, 147, 980
HpaII CCGG 2 cut(s) 462, 1008
HphI GGTGA 2 cut(s) 791, 863
Hpy166II GTNNAC 3 cut(s) 184, 928, 1017
Hpy188I TCNGA 5 cut(s) 146, 315, 483, 721, 816
Hpy188III TCNNGA 6 cut(s) 133, 494, 770, 920, 968, 977
Hpy8I GTNNAC 3 cut(s) 184, 928, 1017
Hpy99I CGWCG 3 cut(s) 107, 128, 299
HpyAV CCTTC 3 cut(s) 11, 246, 778
HpyCH4III ACNGT 3 cut(s) 269, 895, 932
HpyCH4IV ACGT 4 cut(s) 105, 180, 321, 1060
HpyCH4V TGCA 9 cut(s) 258, 368, 387, 502, 515, 544, 658, 838, 857
HpyF10VI GCNNNNNNNGC 5 cut(s) 63, 220, 458, 671, 959
HpyF3I CTNAG 4 cut(s) 275, 312, 480, 495
HpySE526I ACGT 4 cut(s) 105, 180, 321, 1060
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 6 cut(s) 387, 533, 544, 662, 923, 958
HspAI GCGC 5 cut(s) 208, 210, 212, 653, 960
KroI GCCGGC 1 cut(s) 461
KroNI GCCGGC 1 cut(s) 463
Kzo9I GATC 2 cut(s) 60, 689
LmnI GCTCC 5 cut(s) 46, 205, 305, 313, 340
Lsp1109I GCAGC 5 cut(s) 207, 412, 562, 565, 642
LweI GCATC 2 cut(s) 820, 844
MaeI CTAG 2 cut(s) 351, 1028
MaeII ACGT 4 cut(s) 105, 180, 321, 1060
MaeIII GTNAC 1 cut(s) 284
MalI GATC 2 cut(s) 62, 691
MbiI CCGCTC 1 cut(s) 54
MboI GATC 2 cut(s) 60, 689
MboII GAAGA 4 cut(s) 149, 829, 1068, 1078
MhlI GDGCHC 2 cut(s) 345, 747
MluCI AATT 5 cut(s) 394, 773, 859, 885, 914
MlyI GAGTC 1 cut(s) 130
MnlI CCTC 9 cut(s) 121, 173, 321, 335, 418, 544, 586, 916, 1062
MroNI GCCGGC 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 2 cut(s) 462, 1008
MspR9I CCNGG 4 cut(s) 44, 83, 338, 1008
MvaI CCWGG 3 cut(s) 44, 83, 338
MvnI CGCG 2 cut(s) 210, 212
MwoI GCNNNNNNNGC 5 cut(s) 63, 220, 458, 671, 959
NaeI GCCGGC 1 cut(s) 463
NciI CCSGG 1 cut(s) 1008
NdeII GATC 2 cut(s) 60, 689
NgoMIV GCCGGC 1 cut(s) 461
NheI GCTAGC 1 cut(s) 350
NlaIII CATG 6 cut(s) 387, 533, 544, 662, 923, 958
NlaIV GGNNCC 1 cut(s) 301
PagI TCATGA 1 cut(s) 919
PauI GCGCGC 2 cut(s) 208, 210
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PdiI GCCGGC 1 cut(s) 463
PfeI GAWTC 2 cut(s) 147, 980
PkrI GCNGC 8 cut(s) 56, 222, 356, 402, 451, 577, 580, 657
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
Psp124BI GAGCTC 1 cut(s) 345
Psp6I CCWGG 3 cut(s) 42, 81, 336
PspFI CCCAGC 1 cut(s) 949
PspGI CCWGG 3 cut(s) 42, 81, 336
PspN4I GGNNCC 1 cut(s) 301
PspPI GGNCC 2 cut(s) 334, 947
PstNI CAGNNNCTG 2 cut(s) 220, 983
PteI GCGCGC 2 cut(s) 208, 210
PvuII CAGCTG 1 cut(s) 403
RsaI GTAC 1 cut(s) 534
RsaNI GTAC 1 cut(s) 533
SacI GAGCTC 1 cut(s) 345
SatI GCNGC 8 cut(s) 55, 221, 355, 401, 450, 576, 579, 656
Sau3AI GATC 2 cut(s) 60, 689
Sau96I GGNCC 2 cut(s) 334, 947
SchI GAGTC 1 cut(s) 130
ScrFI CCNGG 4 cut(s) 44, 83, 338, 1008
SduI GDGCHC 2 cut(s) 345, 747
SfaNI GCATC 2 cut(s) 820, 844
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 5 cut(s) 394, 773, 859, 885, 914
SsiI CCGC 6 cut(s) 8, 14, 35, 54, 354, 449
SspI AATATT 1 cut(s) 623
SspMI CTAG 2 cut(s) 351, 1028
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 4 cut(s) 42, 81, 336, 1006
StyI CCWWGG 3 cut(s) 192, 634, 943
TaaI ACNGT 3 cut(s) 269, 895, 932
TaiI ACGT 4 cut(s) 108, 183, 324, 1063
TaqI TCGA 2 cut(s) 126, 443
TasI AATT 5 cut(s) 394, 773, 859, 885, 914
TatI WGTACW 1 cut(s) 532
TauI GCSGC 3 cut(s) 57, 357, 452
TfiI GAWTC 2 cut(s) 147, 980
TscAI CASTG 5 cut(s) 287, 352, 375, 551, 654
TseI GCWGC 5 cut(s) 220, 400, 575, 578, 655
TspDTI ATGAA 3 cut(s) 518, 852, 908
TspRI CASTG 5 cut(s) 287, 352, 375, 551, 654
XagI CCTNNNNNAGG 1 cut(s) 329
XapI RAATTY 1 cut(s) 859
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XspI CTAG 2 cut(s) 351, 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.