Rmu_sc0000497.1_g000007

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000497.1
OG0014609
Physical Location & Seq
Forward (+)
38828 .. 42953
4126 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2715 bp
atgcttggagtcctcggttccatttgggtttttgtctgcaactacttgttctttgtgtctggattcattagtagatacatacttatcagatttcaggcagatgatctcagaaatcataaccctgttgtttgtttggagtgtgatcatcaatttgatgcaaatagagacgaaaaagaacaggtgtctttggattctcctattataagaagaaatgatgatcaaaacagagcagaaacagaggattccgatcaaaaaagaacagaaagagaggattccgatgatcaaaacagagcagaaacagaggattccgatcaaaaaagaacagaaagagaggattccggtcaaaacagagcagaaacgaagaattccgatcaaaacagagcagaaacagagggttccgatcaaaacagagcagaaacagaggatttcgatcaaaacagagcagaaacgaataattcagatcaaaacagagcagaaacggagaattccgatcaaaacagagcagaaacagagaattccggttttgtggagaccgtctctgctgtaaccaccgatttgtatgagtatacttctaatagagttatcaatggctatatggaagaaccaaaagccacgagctttattgtccaagaattctatgttgattggggcagttctaaattctctgattatcaaattcctattaaagcagcagaggatgatgatgctgatcaggaaaaagtagtggattctattgaaagcgttgcggccttagagaatggagagcacgagaaaccagtcactgatcaaaaccctcttgaggaattcagtactgggaacgttttagatactgaaattgagctttgtgaggttgaagaagacaatttctctgtagtttctgattcgaaacaagacccggttagctcaagaaaagatcatgatgtgggttcacttaactacgagtttttgatatacagaaatgtggttcagtttgatgaggggcttgaccctcaaagctctggattccagtatgatgaggaagatcatagagtccactttgatatggaagatgatcagttgcttgatgtaagtatcccagacggaaaagcagttgtcttttccgatcaactcgatggttctgaatctcctgatgatgaatacattgaattgttagagccccaattgagaagttatggtcaacgacttgatcaagttgtggagagaggtgaagataaagaggaactagtccaagttgagacagaagccacgagtccactagtacaatctgcacaagaaaaaataggattgcagcctaaaaagtttggattgtattcggatgatgaaaacgaattggagtctcctgacgagcatgatgatctaattgaacagataaaaagggaaattaagaatgtgagaaccggagggcttcccactatttcagaagaagatcattcggagtctacaagaatggtggagaatctaaagcttaagccactgaagattgatgaaaagctcgaatacaaagatcgaatcgcagagattcagaaggtgtacaaaagctactcagagaaaatgcgaaagctggacattttgaacgaccagaccatgcatgcaattggttttctacagctgaaagaccaagttaagccaatttcattggaaaagtcttccattaatccaattatgaagtctcttctttcgcagaacctgttgaggtgtaaagcgcaaagacgaccgacagttgaccctatgccgaaatttgtgggagacttgcacaaggacttggaattggtctatgttggacaggtttgcctctcttgggaaattcttcactggcagcacagaaaagccgaagagttgcagcaatatgatcatcatcactataatgttgttgccactgagtttcaactgtttcaagtgctcctgcaaagattcatagaagatgatccatttcaaggccctcgagtccagcactatgtccggaaccgatgtgtccttcgtagtcttcttcaagttccagccatcagagatgattgcaggaaggataagaagcatgacatgggaggcgaggaggatttgataatcttaagtgagatgctggtgaaaatcattgaagaaacgatgcagatattttggaaatttcttcgcaaggatgagagaaatgcggtcctaaagagccttcaagaaagtcaggttgagcataaggacttggagcttcttgtagatatccggagggatcttcaaaagaaggagaaaaaactcaaagacatacagaggattggaaattgtgtagtgaagaagttccaaaagcagcatcaagatcaagacagactcgagcatagtttttttgtcgctcaagttgaactgaggttggtttcaagagtgctcaacatgtcaaaactaacatcagatcagttagtttggtgccatgaaaagctagacaacatcaattttgttcacagaaggctaggaggtgagggagagggatttcgagagagggtcatggtgttgggagaggttgaggtcgttccacagaggacagtggggatttggccgcagtggatgaggattgtcgtgacggtaatcgcgattcatcggtggatattggtcatggacgtggctcttccatggaaggggattgcttgggcagggcttgtttgtcgatgcgggtttaaggagaccggtgtcgctggaccttggccgcttcacttctttctggcatacttttgctcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

904

Amino Acids

105.0

Weight (kDa)

4.77

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 586 - 817 1.7e-84 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014609)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69610 AT1G69610 AT1G69610
fragaria_vesca FvH4_4g31360
malus_domestica MD16G1072900.v1.1
prunus_persica Prupe.1G274600_v2.0.a1
pyrus_communis pycom16g06320
rosa_chinensis RchiOBHm_Chr4g0439871
rosa_laevigata RLG00000006231
rosa_multiflora Rmu_sc0000497.1_g000007
rosa_roxburghii Rroxscaffold_5G00380780
rosa_rugosa Rorug04G0321800
rosa_samantha Rh4AG372900 Rh4BG385300 Rh4CG399800 Rh4DG379500
rosa_wichuraiana Rw4G031880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
AccB1I GGYRCC 1 cut(s) 2403
AccI GTMKAC 2 cut(s) 566, 1439
AccII CGCG 1 cut(s) 2567
AccIII TCCGGA 2 cut(s) 1959, 2208
AciI CCGC 5 cut(s) 746, 2144, 2534, 2646, 2681
AclI AACGTT 1 cut(s) 819
AclWI GGATC 2 cut(s) 1919, 2223
AcoI YGGCCR 2 cut(s) 2531, 2678
AcuI CTGAAG 1 cut(s) 1496
AfaI GTAC 3 cut(s) 811, 1260, 1532
AfiI CCNNNNNNNGG 4 cut(s) 799, 1798, 1934, 2612
AflII CTTAAG 2 cut(s) 1466, 2065
AflIII ACRYGT 1 cut(s) 2370
AgeI ACCGGT 1 cut(s) 2660
AhlI ACTAGT 2 cut(s) 1222, 1255
AjiI CACGTC 1 cut(s) 2596
Alw21I GWGCWC 3 cut(s) 768, 1902, 2367
Alw26I GTCTC 8 cut(s) 159, 524, 541, 1229, 1341, 1674, 1740, 2651
AlwI GGATC 2 cut(s) 1919, 2223
AlwNI CAGNNNCTG 2 cut(s) 782, 1687
Ama87I CYCGRG 2 cut(s) 1941, 2312
Aor13HI TCCGGA 2 cut(s) 1959, 2208
AoxI GGCC 4 cut(s) 747, 1936, 2531, 2678
ApeKI GCWGC 5 cut(s) 689, 1288, 1816, 1840, 2290
AseI ATTAAT 1 cut(s) 1653
AsiGI ACCGGT 1 cut(s) 2660
Asp700I GAANNNNTTC 2 cut(s) 1806, 2279
AspLEI GCGC 1 cut(s) 1705
AspS9I GGNCC 3 cut(s) 1937, 2146, 2672
AsuC2I CCSGG 1 cut(s) 896
AsuHPI GGTGA 3 cut(s) 1217, 2092, 2465
AsuII TTCGAA 1 cut(s) 884
AvaI CYCGRG 2 cut(s) 1941, 2312
AvaII GGWCC 2 cut(s) 2146, 2672
BanI GGYRCC 1 cut(s) 2403
BanII GRGCYC 1 cut(s) 1158
BauI CACGAG 3 cut(s) 613, 767, 1246
BbsI GAAGAC 3 cut(s) 864, 1638, 1976
Bbv12I GWGCWC 3 cut(s) 768, 1902, 2367
BbvI GCAGC 5 cut(s) 701, 1300, 1828, 1852, 2302
BccI CCATC 2 cut(s) 1106, 2009
BciVI GTATCC 1 cut(s) 1082
BclI TGATCA 8 cut(s) 142, 217, 280, 709, 784, 1051, 1186, 1849
BcnI CCSGG 1 cut(s) 896
BcoDI GTCTC 8 cut(s) 159, 524, 541, 1229, 1341, 1674, 1740, 2651
BcuI ACTAGT 2 cut(s) 1222, 1255
BfaI CTAG 4 cut(s) 1223, 1256, 2417, 2447
BfmI CTRYAG 2 cut(s) 870, 1604
BfrI CTTAAG 2 cut(s) 1466, 2065
BfuI GTATCC 1 cut(s) 1082
BisI GCNGC 8 cut(s) 690, 747, 1289, 1817, 1841, 2291, 2534, 2681
BlsI GCNGC 8 cut(s) 691, 748, 1290, 1818, 1842, 2292, 2535, 2682
BmcAI AGTACT 1 cut(s) 811
Bme1390I CCNGG 1 cut(s) 896
Bme18I GGWCC 2 cut(s) 2146, 2672
BmeT110I CYCGRG 2 cut(s) 1941, 2312
BmgBI CACGTC 1 cut(s) 2596
BmgT120I GGNCC 3 cut(s) 1937, 2146, 2672
BmiI GGNNCC 4 cut(s) 19, 397, 1964, 2405
BmrFI CCNGG 1 cut(s) 896
BmrI ACTGGG 1 cut(s) 822
BmsI GCATC 6 cut(s) 145, 694, 2064, 2091, 2302, 2633
BmuI ACTGGG 1 cut(s) 822
BoxI GACNNNNGTC 1 cut(s) 2663
BpiI GAAGAC 3 cut(s) 864, 1638, 1976
BplI GAGNNNNNCTC 2 cut(s) 521, 553
Bpu14I TTCGAA 1 cut(s) 884
BpuEI CTTGAG 3 cut(s) 818, 889, 2319
BpuMI CCSGG 1 cut(s) 896
BsaBI GATNNNNATC 5 cut(s) 246, 309, 429, 708, 1854
BsaI GGTCTC 2 cut(s) 524, 2651
BsaJI CCNNGG 3 cut(s) 13, 2606, 2675
BsaWI WCCGGW 6 cut(s) 338, 518, 1397, 1959, 2208, 2660
Bsc4I CCNNNNNNNGG 4 cut(s) 799, 1798, 1934, 2612
Bse118I RCCGGY 1 cut(s) 2660
Bse1I ACTGG 4 cut(s) 776, 817, 1006, 1817
Bse8I GATNNNNATC 5 cut(s) 246, 309, 429, 708, 1854
BseAI TCCGGA 2 cut(s) 1959, 2208
BseDI CCNNGG 3 cut(s) 13, 2606, 2675
BseGI GGATG 4 cut(s) 703, 1321, 2137, 2547
BseJI GATNNNNATC 5 cut(s) 246, 309, 429, 708, 1854
BseLI CCNNNNNNNGG 4 cut(s) 799, 1798, 1934, 2612
BseMII CTCAG 4 cut(s) 121, 1557, 1869, 2336
BseNI ACTGG 4 cut(s) 776, 817, 1006, 1817
BseRI GAGGAG 1 cut(s) 2063
BseXI GCAGC 5 cut(s) 701, 1300, 1828, 1852, 2302
BsgI GTGCAG 1 cut(s) 1251
Bsh1236I CGCG 1 cut(s) 2567
Bsh1285I CGRYCG 1 cut(s) 1715
BshFI GGCC 4 cut(s) 749, 1938, 2533, 2680
BshNI GGYRCC 1 cut(s) 2403
BshTI ACCGGT 1 cut(s) 2660
BsiEI CGRYCG 1 cut(s) 1715
BsiHKAI GWGCWC 3 cut(s) 768, 1902, 2367
BsiHKCI CYCGRG 2 cut(s) 1941, 2312
BsiSI CCGG 7 cut(s) 339, 519, 896, 1398, 1960, 2209, 2661
BslI CCNNNNNNNGG 4 cut(s) 799, 1798, 1934, 2612
BsmAI GTCTC 8 cut(s) 159, 524, 541, 1229, 1341, 1674, 1740, 2651
BsmBI CGTCTC 2 cut(s) 159, 541
BsnI GGCC 4 cut(s) 749, 1938, 2533, 2680
Bso31I GGTCTC 2 cut(s) 524, 2651
BsoBI CYCGRG 2 cut(s) 1941, 2312
Bsp119I TTCGAA 1 cut(s) 884
Bsp1286I GDGCHC 4 cut(s) 768, 1158, 1902, 2367
Bsp13I TCCGGA 2 cut(s) 1959, 2208
Bsp1407I TGTACA 1 cut(s) 1530
Bsp19I CCATGG 1 cut(s) 2606
Bsp68I TCGCGA 1 cut(s) 2567
BspACI CCGC 5 cut(s) 746, 2144, 2534, 2646, 2681
BspANI GGCC 4 cut(s) 749, 1938, 2533, 2680
BspCNI CTCAG 4 cut(s) 120, 1556, 1870, 2337
BspEI TCCGGA 2 cut(s) 1959, 2208
BspFNI CGCG 1 cut(s) 2567
BspHI TCATGA 1 cut(s) 916
BspLI GGNNCC 4 cut(s) 19, 397, 1964, 2405
BspPI GGATC 2 cut(s) 1919, 2223
BspQI GCTCTTC 1 cut(s) 2607
BspT104I TTCGAA 1 cut(s) 884
BspT107I GGYRCC 1 cut(s) 2403
BspTI CTTAAG 2 cut(s) 1466, 2065
BspTNI GGTCTC 2 cut(s) 524, 2651
BsrFI RCCGGY 1 cut(s) 2660
BsrGI TGTACA 1 cut(s) 1530
BsrI ACTGG 4 cut(s) 776, 817, 1006, 1817
BssAI RCCGGY 1 cut(s) 2660
BssECI CCNNGG 3 cut(s) 13, 2606, 2675
BssNAI GTATAC 1 cut(s) 567
BssSI CACGAG 3 cut(s) 613, 767, 1246
BssT1I CCWWGG 2 cut(s) 2606, 2675
Bst1107I GTATAC 1 cut(s) 567
Bst2BI CACGAG 3 cut(s) 613, 767, 1246
Bst4CI ACNGT 5 cut(s) 535, 1720, 1890, 2521, 2560
Bst6I CTCTTC 3 cut(s) 1677, 1827, 2607
BstAFI CTTAAG 2 cut(s) 1466, 2065
BstAUI TGTACA 1 cut(s) 1530
BstBI TTCGAA 1 cut(s) 884
BstC8I GCNNGC 1 cut(s) 1590
BstDEI CTNAG 5 cut(s) 107, 751, 1543, 1878, 2345
BstDSI CCRYGG 1 cut(s) 2606
BstF5I GGATG 4 cut(s) 703, 1321, 2137, 2547
BstFNI CGCG 1 cut(s) 2567
BstHHI GCGC 1 cut(s) 1705
BstMAI GTCTC 8 cut(s) 159, 524, 541, 1229, 1341, 1674, 1740, 2651
BstMCI CGRYCG 1 cut(s) 1715
BstNSI RCATGY 2 cut(s) 1592, 2374
BstPAI GACNNNNGTC 1 cut(s) 2663
BstSCI CCNGG 1 cut(s) 894
BstSFI CTRYAG 2 cut(s) 870, 1604
BstUI CGCG 1 cut(s) 2567
BstV1I GCAGC 5 cut(s) 701, 1300, 1828, 1852, 2302
BstV2I GAAGAC 3 cut(s) 864, 1638, 1976
BstX2I RGATCY 1 cut(s) 2215
BstYI RGATCY 1 cut(s) 2215
BstZ17I GTATAC 1 cut(s) 567
BsuI GTATCC 1 cut(s) 1082
BsuRI GGCC 4 cut(s) 749, 1938, 2533, 2680
BtgI CCRYGG 1 cut(s) 2606
BtrI CACGTC 1 cut(s) 2596
BtsCI GGATG 4 cut(s) 703, 1321, 2137, 2547
BtsI GCAGTG 1 cut(s) 2543
BtsIMutI CAGTG 6 cut(s) 780, 1472, 1810, 1875, 2526, 2543
BtuMI TCGCGA 1 cut(s) 2567
Cac8I GCNNGC 1 cut(s) 1590
CaiI CAGNNNCTG 2 cut(s) 782, 1687
CciI TCATGA 1 cut(s) 916
CfoI GCGC 1 cut(s) 1705
Cfr10I RCCGGY 1 cut(s) 2660
Cfr13I GGNCC 3 cut(s) 1937, 2146, 2672
Csp6I GTAC 3 cut(s) 810, 1259, 1531
CspAI ACCGGT 1 cut(s) 2660
CspCI CAANNNNNGTGG 2 cut(s) 1431, 1466
CviQI GTAC 3 cut(s) 810, 1259, 1531
DdeI CTNAG 5 cut(s) 107, 751, 1543, 1878, 2345
EaeI YGGCCR 2 cut(s) 2531, 2678
Eam1104I CTCTTC 3 cut(s) 1677, 1827, 2607
EarI CTCTTC 3 cut(s) 1677, 1827, 2607
Eco130I CCWWGG 2 cut(s) 2606, 2675
Eco24I GRGCYC 1 cut(s) 1158
Eco31I GGTCTC 2 cut(s) 524, 2651
Eco32I GATATC 1 cut(s) 2206
Eco47I GGWCC 2 cut(s) 2146, 2672
Eco57I CTGAAG 1 cut(s) 1496
Eco88I CYCGRG 2 cut(s) 1941, 2312
EcoO109I RGGNCCY 1 cut(s) 1937
EcoRI GAATTC 5 cut(s) 364, 484, 514, 632, 803
EcoRV GATATC 1 cut(s) 2206
EcoT14I CCWWGG 2 cut(s) 2606, 2675
EcoT22I ATGCAT 1 cut(s) 1590
EcoT38I GRGCYC 1 cut(s) 1158
ErhI CCWWGG 2 cut(s) 2606, 2675
Esp3I CGTCTC 2 cut(s) 159, 541
FauI CCCGC 1 cut(s) 2639
FbaI TGATCA 8 cut(s) 142, 217, 280, 709, 784, 1051, 1186, 1849
FblI GTMKAC 2 cut(s) 566, 1439
Fnu4HI GCNGC 8 cut(s) 690, 747, 1289, 1817, 1841, 2291, 2534, 2681
FokI GGATG 4 cut(s) 710, 1328, 2144, 2554
FriOI GRGCYC 1 cut(s) 1158
Fsp4HI GCNGC 8 cut(s) 690, 747, 1289, 1817, 1841, 2291, 2534, 2681
FspBI CTAG 4 cut(s) 1223, 1256, 2417, 2447
GlaI GCGC 1 cut(s) 1704
GluI GCNGC 8 cut(s) 690, 747, 1289, 1817, 1841, 2291, 2534, 2681
HaeIII GGCC 4 cut(s) 749, 1938, 2533, 2680
HapII CCGG 7 cut(s) 339, 519, 896, 1398, 1960, 2209, 2661
HhaI GCGC 1 cut(s) 1705
Hin6I GCGC 1 cut(s) 1703
HinP1I GCGC 1 cut(s) 1703
HincII GTYRAC 2 cut(s) 1178, 1723
HindII GTYRAC 2 cut(s) 1178, 1723
HindIII AAGCTT 1 cut(s) 1463
HpaII CCGG 7 cut(s) 339, 519, 896, 1398, 1960, 2209, 2661
HphI GGTGA 3 cut(s) 1217, 2092, 2465
Hpy166II GTNNAC 9 cut(s) 567, 929, 1033, 1178, 1253, 1440, 1531, 1723, 2437
Hpy8I GTNNAC 9 cut(s) 567, 929, 1033, 1178, 1253, 1440, 1531, 1723, 2437
HpyAV CCTTC 7 cut(s) 1519, 1985, 2014, 2168, 2221, 2436, 2605
HpyCH4III ACNGT 5 cut(s) 535, 1720, 1890, 2521, 2560
HpyCH4IV ACGT 2 cut(s) 819, 2595
HpyF3I CTNAG 5 cut(s) 107, 751, 1543, 1878, 2345
HpySE526I ACGT 2 cut(s) 819, 2595
HspAI GCGC 1 cut(s) 1703
Kpn2I TCCGGA 2 cut(s) 1959, 2208
Ksp22I TGATCA 8 cut(s) 142, 217, 280, 709, 784, 1051, 1186, 1849
LguI GCTCTTC 1 cut(s) 2607
LmnI GCTCC 2 cut(s) 1905, 2191
Lsp1109I GCAGC 5 cut(s) 701, 1300, 1828, 1852, 2302
LweI GCATC 6 cut(s) 145, 694, 2064, 2091, 2302, 2633
MaeI CTAG 4 cut(s) 1223, 1256, 2417, 2447
MaeII ACGT 2 cut(s) 819, 2595
MaeIII GTNAC 3 cut(s) 544, 778, 2554
MfeI CAATTG 2 cut(s) 1160, 1593
MflI RGATCY 1 cut(s) 2215
MhlI GDGCHC 4 cut(s) 768, 1158, 1902, 2367
MlyI GAGTC 7 cut(s) 18, 1038, 1258, 1343, 1445, 1953, 2304
MmeI TCCRAC 1 cut(s) 1759
Mph1103I ATGCAT 1 cut(s) 1590
MroI TCCGGA 2 cut(s) 1959, 2208
MroXI GAANNNNTTC 2 cut(s) 1806, 2279
MseI TTAA 8 cut(s) 684, 933, 1383, 1467, 1623, 1653, 2066, 2652
MslI CAYNNNNRTG 2 cut(s) 1863, 2594
MspA1I CMGCKG 1 cut(s) 1609
MspCI CTTAAG 2 cut(s) 1466, 2065
MspI CCGG 7 cut(s) 339, 519, 896, 1398, 1960, 2209, 2661
MspR9I CCNGG 1 cut(s) 896
MunI CAATTG 2 cut(s) 1160, 1593
MvnI CGCG 1 cut(s) 2567
NciI CCSGG 1 cut(s) 896
NcoI CCATGG 1 cut(s) 2606
NlaIV GGNNCC 4 cut(s) 19, 397, 1964, 2405
NmuCI GTSAC 2 cut(s) 778, 2554
NruI TCGCGA 1 cut(s) 2567
NsiI ATGCAT 1 cut(s) 1590
NspI RCATGY 2 cut(s) 1592, 2374
NspV TTCGAA 1 cut(s) 884
PaeI GCATGC 1 cut(s) 1592
PaeR7I CTCGAG 2 cut(s) 1941, 2312
PagI TCATGA 1 cut(s) 916
PciI ACATGT 1 cut(s) 2370
PciSI GCTCTTC 1 cut(s) 2607
PcsI WCGNNNNNNNCGW 1 cut(s) 2564
PdmI GAANNNNTTC 2 cut(s) 1806, 2279
PinAI ACCGGT 1 cut(s) 2660
PkrI GCNGC 8 cut(s) 691, 748, 1290, 1818, 1842, 2292, 2535, 2682
PleI GAGTC 7 cut(s) 17, 1037, 1257, 1342, 1444, 1952, 2304
PpsI GAGTC 7 cut(s) 17, 1037, 1257, 1342, 1444, 1952, 2304
PscI ACATGT 1 cut(s) 2370
PshAI GACNNNNGTC 1 cut(s) 2663
PshBI ATTAAT 1 cut(s) 1653
PsiI TTATAA 1 cut(s) 203
Psp1406I AACGTT 1 cut(s) 819
PspN4I GGNNCC 4 cut(s) 19, 397, 1964, 2405
PspPI GGNCC 3 cut(s) 1937, 2146, 2672
PspXI VCTCGAGB 2 cut(s) 1941, 2312
PstNI CAGNNNCTG 2 cut(s) 782, 1687
PsuI RGATCY 1 cut(s) 2215
PvuII CAGCTG 1 cut(s) 1609
RruI TCGCGA 1 cut(s) 2567
RsaI GTAC 3 cut(s) 811, 1260, 1532
RsaNI GTAC 3 cut(s) 810, 1259, 1531
RseI CAYNNNNRTG 2 cut(s) 1863, 2594
SapI GCTCTTC 1 cut(s) 2607
SaqAI TTAA 8 cut(s) 684, 933, 1383, 1467, 1623, 1653, 2066, 2652
SatI GCNGC 8 cut(s) 690, 747, 1289, 1817, 1841, 2291, 2534, 2681
Sau96I GGNCC 3 cut(s) 1937, 2146, 2672
ScaI AGTACT 1 cut(s) 811
SchI GAGTC 7 cut(s) 18, 1038, 1258, 1343, 1445, 1953, 2304
ScrFI CCNGG 1 cut(s) 896
SduI GDGCHC 4 cut(s) 768, 1158, 1902, 2367
SfaNI GCATC 6 cut(s) 145, 694, 2064, 2091, 2302, 2633
SfcI CTRYAG 2 cut(s) 870, 1604
Sfr274I CTCGAG 2 cut(s) 1941, 2312
SfuI TTCGAA 1 cut(s) 884
SinI GGWCC 2 cut(s) 2146, 2672
SlaI CTCGAG 2 cut(s) 1941, 2312
SmiMI CAYNNNNRTG 2 cut(s) 1863, 2594
SmlI CTYRAG 7 cut(s) 797, 904, 1466, 1941, 2065, 2312, 2334
SmoI CTYRAG 7 cut(s) 797, 904, 1466, 1941, 2065, 2312, 2334
SpeI ACTAGT 2 cut(s) 1222, 1255
SphI GCATGC 1 cut(s) 1592
SsiI CCGC 5 cut(s) 746, 2144, 2534, 2646, 2681
SspMI CTAG 4 cut(s) 1223, 1256, 2417, 2447
StyD4I CCNGG 1 cut(s) 894
StyI CCWWGG 2 cut(s) 2606, 2675
TaaI ACNGT 5 cut(s) 535, 1720, 1890, 2521, 2560
TaiI ACGT 2 cut(s) 822, 2598
TaqI TCGA 9 cut(s) 429, 884, 1110, 1494, 1507, 1942, 2313, 2470, 2641
TaqII GACCGA 1 cut(s) 1729
TatI WGTACW 3 cut(s) 809, 1258, 1530
TauI GCSGC 3 cut(s) 749, 2536, 2683
Tru1I TTAA 8 cut(s) 684, 933, 1383, 1467, 1623, 1653, 2066, 2652
Tru9I TTAA 8 cut(s) 684, 933, 1383, 1467, 1623, 1653, 2066, 2652
TscAI CASTG 6 cut(s) 787, 1479, 1817, 1882, 2526, 2543
TseFI GTSAC 2 cut(s) 778, 2554
TseI GCWGC 5 cut(s) 689, 1288, 1816, 1840, 2290
Tsp45I GTSAC 2 cut(s) 778, 2554
TspDTI ATGAA 9 cut(s) 55, 1149, 1335, 1500, 1623, 1679, 1903, 2424, 2561
TspGWI ACGGA 2 cut(s) 494, 1095
TspRI CASTG 6 cut(s) 787, 1479, 1817, 1882, 2526, 2543
Vha464I CTTAAG 2 cut(s) 1466, 2065
VpaK11BI GGWCC 2 cut(s) 2146, 2672
VspI ATTAAT 1 cut(s) 1653
XceI RCATGY 2 cut(s) 1592, 2374
XhoI CTCGAG 2 cut(s) 1941, 2312
XmiI GTMKAC 2 cut(s) 566, 1439
XmnI GAANNNNTTC 2 cut(s) 1806, 2279
XspI CTAG 4 cut(s) 1223, 1256, 2417, 2447
ZrmI AGTACT 1 cut(s) 811
Zsp2I ATGCAT 1 cut(s) 1590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.