Rmu_sc0000547.1_g000013

dnaJ homolog subfamily C member 25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000547.1
OG0013208
Physical Location & Seq
Reverse (-)
48968 .. 53038
4071 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1128 bp
atgaatgaaggaataagagagagaaaaattgggtcggcccgattggaggctggggtgtggttacgatgcagggctcggcccagagaagcttgggcggctgacagtgacttggcttggaggaatggcaactacggtttatctgggcttttgggcaataggcagagagagagagagtgtttgcagggaaaccatttcagaggaaatctagagagctccatcgtcgccatggcgccaccggcatcgattcggtggttcgcggccgcttcagctctaatgctctgccttctcatctctccatccaccgccatctactgcgacgaagacgactgctacgatctcctcggggttacgcagagcgcgaactcgtcggaaatcaagaaagcgtactataagctatctttgaagcatcacccggataagaacccggatccggaatcgaaaaagctgtttgttaaaattgcaaatgcttatgagattctgaaggacgaggcgtcgcgggagcagtatgattatgcaattgcacatcctgaggagtatttttacaatacagctcgctactatcaagcatattatggtcacaagacggatcctcgtgctgtactggttggctttcttttggttctttcagcatttcagtatttcaatcaatggacaaggtataatcaggctatagacatggttaaaaagacgcctgcttacaaaaatagacttcggcaattggaacttgagcgtagtggggggactacaactaagaagaagagtaacaagcagacaaacaagaaaatggatgaagatctcagcaaagaacttgaattgcagattacaggtgctgagcaaccctccatgtgggatcttcttggtgtccgtttcatactgttaccctacactattggcaagctgctgttatggcatggctgttggttctggagatacaaggtgaaacaagctccatatacatgggatgatgcctcctacctgacacagagatcccttagtgcgccacttgacgcatggagaaatgttgatgacttacgaaaggaagatcttatcaatagacgtttatgggaaggttcgaacttggagagctattatgcggagatgcgaaaagaatccaagcgcagaagatag

Protein Analysis

375

Amino Acids

43.69

Weight (kDa)

9.17

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 109 - 170 9e-24 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61770
fragaria_vesca FvH4_3g02660
malus_domestica MD05G1341100.v1.1 MD10G1314600.v1.1
prunus_persica Prupe.4G026200_v2.0.a1 Prupe.4G026200_v2.0.a1
pyrus_communis pycom05g31040 pycom10g26670
rosa_chinensis RchiOBHm_Chr5g0003981
rosa_laevigata RLG00000031194
rosa_multiflora Rmu_sc0000547.1_g000013
rosa_roxburghii Rroxscaffold_1G00071980
rosa_rugosa Rorug04G0408700
rosa_samantha Rh5AG036400 Rh5BG035500 Rh5CG039100 Rh5DG035000
rosa_wichuraiana Rw5G003310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 229
AccII CGCG 3 cut(s) 257, 359, 496
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 6 cut(s) 95, 257, 261, 303, 496, 1094
AclWI GGATC 6 cut(s) 422, 435, 581, 594, 858, 981
AcoI YGGCCR 1 cut(s) 258
AcuI CTGAAG 2 cut(s) 249, 500
AcyI GRCGYC 3 cut(s) 230, 491, 689
AfaI GTAC 2 cut(s) 386, 600
AfiI CCNNNNNNNGG 3 cut(s) 46, 430, 846
AgsI TTSAA 3 cut(s) 403, 643, 812
AhdI GACNNNNNGTC 1 cut(s) 490
AluBI AGCT 9 cut(s) 89, 213, 269, 394, 445, 551, 898, 947, 1086
AluI AGCT 9 cut(s) 89, 213, 269, 394, 445, 551, 898, 947, 1086
Alw21I GWGCWC 1 cut(s) 215
AlwI GGATC 6 cut(s) 422, 435, 581, 594, 858, 981
AlwNI CAGNNNCTG 1 cut(s) 830
Ama87I CYCGRG 1 cut(s) 341
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 3 cut(s) 36, 77, 258
ApeKI GCWGC 1 cut(s) 898
AspLEI GCGC 4 cut(s) 232, 359, 1000, 1119
AspS9I GGNCC 2 cut(s) 37, 78
AsuC2I CCSGG 2 cut(s) 413, 425
AsuHPI GGTGA 2 cut(s) 401, 949
AsuII TTCGAA 1 cut(s) 1073
AvaI CYCGRG 1 cut(s) 341
AxyI CCTNAGG 1 cut(s) 528
BamHI GGATCC 2 cut(s) 427, 586
BanI GGYRCC 1 cut(s) 229
BanII GRGCYC 2 cut(s) 76, 215
BauI CACGAG 1 cut(s) 591
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 1 cut(s) 885
BccI CCATC 3 cut(s) 224, 304, 314
BcnI CCSGG 2 cut(s) 413, 425
BfaI CTAG 1 cut(s) 206
BfmI CTRYAG 1 cut(s) 669
BfoI RGCGCY 1 cut(s) 233
BglII AGATCT 2 cut(s) 793, 1042
BisI GCNGC 4 cut(s) 96, 258, 261, 899
BlpI GCTNAGC 1 cut(s) 831
BlsI GCNGC 4 cut(s) 97, 259, 262, 900
Bme1390I CCNGG 2 cut(s) 413, 425
BmeRI GACNNNNNGTC 1 cut(s) 490
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 2 cut(s) 37, 78
BmiI GGNNCC 3 cut(s) 231, 429, 588
BmrFI CCNGG 2 cut(s) 413, 425
BmsI GCATC 5 cut(s) 56, 248, 415, 955, 1089
BpiI GAAGAC 1 cut(s) 327
BplI GAGNNNNNCTC 2 cut(s) 824, 856
BpmI CTGGAG 1 cut(s) 946
Bpu1102I GCTNAGC 1 cut(s) 831
Bpu14I TTCGAA 1 cut(s) 1073
BpuEI CTTGAG 1 cut(s) 746
BpuMI CCSGG 2 cut(s) 413, 425
Bsa29I ATCGAT 1 cut(s) 242
BsaBI GATNNNNATC 1 cut(s) 792
BsaHI GRCGYC 3 cut(s) 230, 491, 689
BsaJI CCNNGG 2 cut(s) 225, 340
BsaWI WCCGGW 1 cut(s) 430
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 430, 846
Bse118I RCCGGY 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 606
Bse21I CCTNAGG 1 cut(s) 528
Bse8I GATNNNNATC 1 cut(s) 792
BseAI TCCGGA 1 cut(s) 430
BseCI ATCGAT 1 cut(s) 242
BseDI CCNNGG 2 cut(s) 225, 340
BseGI GGATG 4 cut(s) 296, 523, 793, 967
BseJI GATNNNNATC 1 cut(s) 792
BseLI CCNNNNNNNGG 3 cut(s) 46, 430, 846
BseMII CTCAG 3 cut(s) 519, 811, 822
BseNI ACTGG 1 cut(s) 606
BseRI GAGGAG 2 cut(s) 329, 545
BseX3I CGGCCG 1 cut(s) 258
BseXI GCAGC 1 cut(s) 885
BseYI CCCAGC 1 cut(s) 50
Bsh1236I CGCG 3 cut(s) 257, 359, 496
Bsh1285I CGRYCG 1 cut(s) 261
BshFI GGCC 3 cut(s) 38, 79, 260
BshNI GGYRCC 1 cut(s) 229
BshVI ATCGAT 1 cut(s) 242
BsiEI CGRYCG 1 cut(s) 261
BsiHKAI GWGCWC 1 cut(s) 215
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 4 cut(s) 236, 413, 425, 431
BslFI GGGAC 1 cut(s) 754
BslI CCNNNNNNNGG 3 cut(s) 46, 430, 846
BsmFI GGGAC 1 cut(s) 754
BsnI GGCC 3 cut(s) 38, 79, 260
BsoBI CYCGRG 1 cut(s) 341
Bsp119I TTCGAA 1 cut(s) 1073
Bsp1286I GDGCHC 2 cut(s) 76, 215
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 7 cut(s) 334, 427, 586, 793, 850, 986, 1042
Bsp1720I GCTNAGC 1 cut(s) 831
Bsp19I CCATGG 1 cut(s) 225
BspACI CCGC 6 cut(s) 95, 257, 261, 303, 496, 1094
BspANI GGCC 3 cut(s) 38, 79, 260
BspCNI CTCAG 3 cut(s) 520, 810, 823
BspDI ATCGAT 1 cut(s) 242
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 3 cut(s) 257, 359, 496
BspLI GGNNCC 3 cut(s) 231, 429, 588
BspPI GGATC 6 cut(s) 422, 435, 581, 594, 858, 981
BspT104I TTCGAA 1 cut(s) 1073
BspT107I GGYRCC 1 cut(s) 229
BsrFI RCCGGY 1 cut(s) 235
BsrI ACTGG 1 cut(s) 606
BssAI RCCGGY 1 cut(s) 235
BssECI CCNNGG 2 cut(s) 225, 340
BssMI GATC 7 cut(s) 334, 427, 586, 793, 850, 986, 1042
BssNI GRCGYC 3 cut(s) 230, 491, 689
BssSI CACGAG 1 cut(s) 591
BssT1I CCWWGG 1 cut(s) 225
Bst2BI CACGAG 1 cut(s) 591
Bst4CI ACNGT 3 cut(s) 104, 134, 876
Bst6I CTCTTC 1 cut(s) 752
BstACI GRCGYC 3 cut(s) 230, 491, 689
BstBI TTCGAA 1 cut(s) 1073
BstC8I GCNNGC 3 cut(s) 553, 693, 896
BstDEI CTNAG 5 cut(s) 528, 750, 797, 831, 992
BstDSI CCRYGG 1 cut(s) 225
BstF5I GGATG 4 cut(s) 296, 523, 793, 967
BstFNI CGCG 3 cut(s) 257, 359, 496
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 4 cut(s) 232, 359, 1000, 1119
BstKTI GATC 7 cut(s) 337, 430, 589, 796, 853, 989, 1045
BstMBI GATC 7 cut(s) 334, 427, 586, 793, 850, 986, 1042
BstMCI CGRYCG 1 cut(s) 261
BstMWI GCNNNNNNNGC 4 cut(s) 95, 236, 266, 907
BstSCI CCNGG 2 cut(s) 411, 423
BstSFI CTRYAG 1 cut(s) 669
BstUI CGCG 3 cut(s) 257, 359, 496
BstV1I GCAGC 1 cut(s) 885
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 6 cut(s) 427, 586, 793, 850, 986, 1042
BstXI CCANNNNNNTGG 1 cut(s) 957
BstYI RGATCY 6 cut(s) 427, 586, 793, 850, 986, 1042
BstZI CGGCCG 1 cut(s) 258
Bsu15I ATCGAT 1 cut(s) 242
Bsu36I CCTNAGG 1 cut(s) 528
BsuRI GGCC 3 cut(s) 38, 79, 260
BsuTUI ATCGAT 1 cut(s) 242
BtgI CCRYGG 1 cut(s) 225
BtsCI GGATG 4 cut(s) 296, 523, 793, 967
BtsIMutI CAGTG 1 cut(s) 109
Cac8I GCNNGC 3 cut(s) 553, 693, 896
CaiI CAGNNNCTG 1 cut(s) 830
CciNI GCGGCCGC 1 cut(s) 258
CfoI GCGC 4 cut(s) 232, 359, 1000, 1119
Cfr10I RCCGGY 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 37, 78
ClaI ATCGAT 1 cut(s) 242
CseI GACGC 3 cut(s) 480, 697, 1016
Csp6I GTAC 2 cut(s) 385, 599
CviAII CATG 6 cut(s) 226, 676, 844, 911, 957, 1011
CviQI GTAC 2 cut(s) 385, 599
DdeI CTNAG 5 cut(s) 528, 750, 797, 831, 992
DinI GGCGCC 1 cut(s) 231
DpnI GATC 7 cut(s) 336, 429, 588, 795, 852, 988, 1044
DpnII GATC 7 cut(s) 334, 427, 586, 793, 850, 986, 1042
DriI GACNNNNNGTC 1 cut(s) 490
EaeI YGGCCR 1 cut(s) 258
EagI CGGCCG 1 cut(s) 258
Eam1104I CTCTTC 1 cut(s) 752
Eam1105I GACNNNNNGTC 1 cut(s) 490
EarI CTCTTC 1 cut(s) 752
Ecl136II GAGCTC 1 cut(s) 213
EclXI CGGCCG 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 225
Eco24I GRGCYC 2 cut(s) 76, 215
Eco52I CGGCCG 1 cut(s) 258
Eco53kI GAGCTC 1 cut(s) 213
Eco57I CTGAAG 2 cut(s) 249, 500
Eco81I CCTNAGG 1 cut(s) 528
Eco88I CYCGRG 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 225
EcoT38I GRGCYC 2 cut(s) 76, 215
EgeI GGCGCC 1 cut(s) 231
EheI GGCGCC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 6 cut(s) 229, 679, 847, 914, 960, 1014
FaqI GGGAC 1 cut(s) 754
FatI CATG 6 cut(s) 225, 675, 843, 910, 956, 1010
FauI CCCGC 1 cut(s) 489
Fnu4HI GCNGC 4 cut(s) 96, 258, 261, 899
FokI GGATG 4 cut(s) 283, 510, 800, 974
FriOI GRGCYC 2 cut(s) 76, 215
Fsp4HI GCNGC 4 cut(s) 96, 258, 261, 899
FspBI CTAG 1 cut(s) 206
GlaI GCGC 4 cut(s) 231, 358, 999, 1118
GluI GCNGC 4 cut(s) 96, 258, 261, 899
GsaI CCCAGC 1 cut(s) 54
GsuI CTGGAG 1 cut(s) 946
HaeII RGCGCY 1 cut(s) 233
HaeIII GGCC 3 cut(s) 38, 79, 260
HapII CCGG 4 cut(s) 236, 413, 425, 431
HgaI GACGC 3 cut(s) 480, 697, 1016
HhaI GCGC 4 cut(s) 232, 359, 1000, 1119
Hin1I GRCGYC 3 cut(s) 230, 491, 689
Hin1II CATG 6 cut(s) 229, 679, 847, 914, 960, 1014
Hin6I GCGC 4 cut(s) 230, 357, 998, 1117
HinP1I GCGC 4 cut(s) 230, 357, 998, 1117
HindIII AAGCTT 1 cut(s) 87
HinfI GANTC 4 cut(s) 244, 434, 475, 1109
HpaII CCGG 4 cut(s) 236, 413, 425, 431
HphI GGTGA 2 cut(s) 401, 949
Hpy188I TCNGA 3 cut(s) 197, 370, 480
Hpy188III TCNNGA 5 cut(s) 206, 376, 431, 527, 925
Hpy99I CGWCG 4 cut(s) 224, 320, 370, 496
HpyAV CCTTC 3 cut(s) 293, 475, 1061
HpyCH4III ACNGT 3 cut(s) 104, 134, 876
HpyCH4IV ACGT 1 cut(s) 1057
HpyCH4V TGCA 6 cut(s) 69, 181, 461, 515, 521, 817
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 236, 266, 907
HpyF3I CTNAG 5 cut(s) 528, 750, 797, 831, 992
HpySE526I ACGT 1 cut(s) 1057
Hsp92I GRCGYC 3 cut(s) 230, 491, 689
Hsp92II CATG 6 cut(s) 229, 679, 847, 914, 960, 1014
HspAI GCGC 4 cut(s) 230, 357, 998, 1117
KasI GGCGCC 1 cut(s) 229
Kpn2I TCCGGA 1 cut(s) 430
Kzo9I GATC 7 cut(s) 334, 427, 586, 793, 850, 986, 1042
LmnI GCTCC 3 cut(s) 218, 499, 952
Lsp1109I GCAGC 1 cut(s) 885
LweI GCATC 5 cut(s) 56, 248, 415, 955, 1089
MaeI CTAG 1 cut(s) 206
MaeII ACGT 1 cut(s) 1057
MaeIII GTNAC 6 cut(s) 60, 104, 346, 575, 761, 876
MalI GATC 7 cut(s) 336, 429, 588, 795, 852, 988, 1044
MboI GATC 7 cut(s) 334, 427, 586, 793, 850, 986, 1042
MboII GAAGA 6 cut(s) 332, 766, 769, 803, 845, 1052
MfeI CAATTG 2 cut(s) 516, 716
MflI RGATCY 6 cut(s) 427, 586, 793, 850, 986, 1042
MhlI GDGCHC 2 cut(s) 76, 215
MluCI AATT 5 cut(s) 27, 456, 516, 716, 812
Mly113I GGCGCC 1 cut(s) 230
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 9 cut(s) 40, 111, 191, 350, 481, 523, 600, 850, 979
MroI TCCGGA 1 cut(s) 430
MseI TTAA 2 cut(s) 453, 681
MslI CAYNNNNRTG 1 cut(s) 955
MspI CCGG 4 cut(s) 236, 413, 425, 431
MspR9I CCNGG 2 cut(s) 413, 425
MunI CAATTG 2 cut(s) 516, 716
MvnI CGCG 3 cut(s) 257, 359, 496
MwoI GCNNNNNNNGC 4 cut(s) 95, 236, 266, 907
NarI GGCGCC 1 cut(s) 230
NciI CCSGG 2 cut(s) 413, 425
NcoI CCATGG 1 cut(s) 225
NdeII GATC 7 cut(s) 334, 427, 586, 793, 850, 986, 1042
NlaIII CATG 6 cut(s) 229, 679, 847, 914, 960, 1014
NlaIV GGNNCC 3 cut(s) 231, 429, 588
NmeAIII GCCGAG 1 cut(s) 55
NmuCI GTSAC 2 cut(s) 104, 575
NotI GCGGCCGC 1 cut(s) 258
NspV TTCGAA 1 cut(s) 1073
PcsI WCGNNNNNNNCGW 2 cut(s) 330, 356
PfeI GAWTC 4 cut(s) 244, 434, 475, 1109
PkrI GCNGC 4 cut(s) 97, 259, 262, 900
PluTI GGCGCC 1 cut(s) 233
Psp124BI GAGCTC 1 cut(s) 215
PspFI CCCAGC 1 cut(s) 50
PspN4I GGNNCC 3 cut(s) 231, 429, 588
PspPI GGNCC 2 cut(s) 37, 78
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 6 cut(s) 427, 586, 793, 850, 986, 1042
RsaI GTAC 2 cut(s) 386, 600
RsaNI GTAC 2 cut(s) 385, 599
RseI CAYNNNNRTG 1 cut(s) 955
SacI GAGCTC 1 cut(s) 215
SaqAI TTAA 2 cut(s) 453, 681
SatI GCNGC 4 cut(s) 96, 258, 261, 899
Sau3AI GATC 7 cut(s) 334, 427, 586, 793, 850, 986, 1042
Sau96I GGNCC 2 cut(s) 37, 78
ScrFI CCNGG 2 cut(s) 413, 425
SduI GDGCHC 2 cut(s) 76, 215
SfaNI GCATC 5 cut(s) 56, 248, 415, 955, 1089
SfcI CTRYAG 1 cut(s) 669
SfoI GGCGCC 1 cut(s) 231
SfuI TTCGAA 1 cut(s) 1073
SmiMI CAYNNNNRTG 1 cut(s) 955
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
Sse9I AATT 5 cut(s) 27, 456, 516, 716, 812
SsiI CCGC 6 cut(s) 95, 257, 261, 303, 496, 1094
SspDI GGCGCC 1 cut(s) 229
SspMI CTAG 1 cut(s) 206
SstI GAGCTC 1 cut(s) 215
StyD4I CCNGG 2 cut(s) 411, 423
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 3 cut(s) 104, 134, 876
TaiI ACGT 1 cut(s) 1060
TaqI TCGA 3 cut(s) 242, 437, 1073
TasI AATT 5 cut(s) 27, 456, 516, 716, 812
TatI WGTACW 1 cut(s) 598
TauI GCSGC 3 cut(s) 98, 260, 263
TfiI GAWTC 4 cut(s) 244, 434, 475, 1109
Tru1I TTAA 2 cut(s) 453, 681
Tru9I TTAA 2 cut(s) 453, 681
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 2 cut(s) 104, 575
TseI GCWGC 1 cut(s) 898
Tsp45I GTSAC 2 cut(s) 104, 575
TspDTI ATGAA 4 cut(s) 17, 21, 804, 859
TspGWI ACGGA 2 cut(s) 599, 854
TspRI CASTG 1 cut(s) 109
XbaI TCTAGA 1 cut(s) 205
XcmI CCANNNNNNNNNTGG 2 cut(s) 223, 1008
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.