Rmu_sc0000573.1_g000051

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000573.1
OG0008578
Physical Location & Seq
Forward (+)
161205 .. 169448
8244 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4209 bp
atggagcggcagaaggagcaaaaacaaggaccgcaaatggggattggtttggagaccttacccagctgcgtattgatcagcgctatcttccccaagctggaactggaaaccctgtgctctctcgcttgcgtcagcagagcaatgaactcctcagtctctcaagccctttcttcactctcctccctcaatctctccgtagaaggtcttaatagcctcaccctcaattgcctgcgcctagacgactcgtcgttggcggtttttctgggtgcacatctccaagaactgaacttgctgtgctgctccttgatgtcgtatcaagtccttgcttccattggagagggttgccccaatctcagggtgcttgggctagaattggtagaccaggaatcacctgaagcttttaagatgaacctggacagaatgcttaatggtagtttatgcttggagtctttatctataaagattcgatggagaggtgtaggtgcaaatcctttacagccatttcgattcttcctgcctaaaactctcaaaagcatcaagttgcagccggtgcttgaggaagacgtaatccatttcactagtttgaatgatgcatttaggaactatttgccaaaagctaatgccagcacccctgtttctaaatcctcatttgcattcgcattgcagcgcttgtcgcttgtcttagatgttatatcagatcagctaattgtcgccattgggatttgtgacaatttggtagagctggatcttgaagatagaccaattcaggaaccattgccaaaccatgacttaaccagcgtggggcttatgagtctggcccctaattttcgtttgaagggattgtctcttgtaagaagtagacagaactgccaagtctcgtttaagggagtggatgatatgggcatgtttgttttttctaagtactgcacgactctagagtcggtgagactttttggtttctccagagttagtgacgcaggctatgcatcagtcctacaatcgtgtcagaatttgaagaaattcgaagtgcgtaatgccttccttatgtcagacttggcctttgttgaccttactcggttctcatgttctcttgttgaggtgaaattgttgtcatgctgtcttataacttgtgaaacggtaaatcagctcaccagttctggggtcctggaggtgcttgatctgtatggctgtaggagcatagcagactcctgcctcatctccattgcatcgcttcatagcttaaccatgctgaatctttcaggagctgatatttctgattacggcttatctgttcttgggagagggaatccacccatcacacatttgtgtcttagaaactgtaagggtgtgactgatgaaggagttttacttttgtttcttggaggaggcacaattggcaaaacactgtcatccctcgatttaggacacataccaggaatatctgataaagcagttctcacaattgctgtggccggtacagggattactgagttgtgtctcagttattgctcctctatgactaattcgtccctagtagctttggctacaaggaaaccggatcgagatgattgtaaactacttcggaggcttgatcttttcaattgcaagggtttgtctgctgactcaattaggttgctgaagaggcctttcttccccggactgcactggcttggtattgggaatactcgtttgaatagtaaaggatgtccagctctcagtgaaattggttacgaaagaccatggctgacgatatgtttggatggctgtgaaatgggcctcgtctctgccggcgaggttttcgagttgggttttttcaaccctggcagacactcaagatggtacttgggcatatggtacatgaaaatcaaagaaagaacagttgtttgggtggccaacagagacaatccgctcccaaacaactcagccagtctcaaaattggatacgacggaatactttctcttgtggatgaatccggaaaagtttcttggatgtcaagcaatcaatcacaatttgttaaaaatccaatccttcagcttttggattccggcaacttggtcctcaaagaagcaaatgaaaccaaccctaacaagttcctctggcagagcttcgactaccctacacacactttgttgccggagatgaagctcggaaggaactcgaataccggattggatagatacataacctcgtggaaaactccacaagacccttctactggtgattattccttcaagctcgactaccgcggtttcccggaggctttcctccggcagagacagtcgataaagtaccggagcggcccctggaacgggttgagttttagcggggctccggagatgaacaccgctagctctaatggtattggtttcaaattcgttgtaaacaatgaggagatgtactactctttttcggaaactaatccagctctgtattcacggctgattgtatctccctccggcaacatccaacggctttcgtggatcgagagccgcaagacctggagccagtgctggtaccttcccagggacccatgcgacacgtacagggagtgcgggccctatggtgtctgcgacgccaacgatgctttgccggtgtgtaagtgcatgaggggattccgacctaagaacccttctgcatggagcttgagagatggcaccgaaggatgtgaacgtcagacggagctggagtgtgggagtaaggacaagtttatgaaggtggagaatttgaagctgccagagagcggcggagcggtggtggatatggagatgggtttggaggagtgtgaggagaagtgtttggggaattgctcgtgtagtgggttttcgagtgcgaggattgagaaaggagggagtggatgtgtgatgtggtttgggaagttgatggatatgaggagttatgccggcaacagcggccaagagttctatctccgattaccagcttctgaactagatggtgatgggaaaacaaaacgcatagttatgagcgtaggcattgtagttggtattgccgttctgctatcagctggtcttattatttggttcgtgcggaggaggaggaactcgggtagcaccctgaggagagaatcaaaaggtcctcttgaaagaattcgaagccaggattttcttctaaacagtgtgttagtctcaagtaagaaggagcactactctggcgacagaagcaatgatgacctagagttgccattgtttgattttacctctatagcagcggctacggagaacttttctgatgaaaataaactgggacaaggtggttttggttgtgtttacaaggggtttgtcgaaggtcaagagatagctgtgaagagactttcaaagaactctggacaaggaaccaaagaattcaaaaacgaagtgaagttaattgcaaggcttcaacacagaaatcttgttcgactgcttggttgctgtgttgatgcggatgaaaagatgctgatttacgaatacatggaaaataaaagcctcgattcctttttattcgataaagcaaaaaggtgtttgctggattggcaaaagcgtttcaacattatctgtgggattgctcgagggcttctttatcttcaccaggattcgagattcagaattatccatagggacctcaaggcaagcaacattctactagatggagaattggaccccaagatatcggactttggaatggcaagactattcggtcaggatcagacagaggccaatacaaaaaaagtagtgggaacatatggctatatgtctcctgaatacgcaatggatggtctcttctcaatcaagtcggatgtatttagttttggtgttctagtattggagattgttagtggtcaaaagaacagaggattctattattcaaacaatgagctaaacctcctaggacatttttgggagctatggaatggaggtcgtgcgttggatataatagattcatcagttggtgattcaatttcagaatctgaggtgttgagatgcatgcaggtagggcttttatgcgtccaagagcgtgcagaagataggcctacaatgtcctctgtgtccatattgcagctcttggacaatgacaatctgatcttgagagatgagagtggtggagattcagaggggtgtgtttttacccgggatatgaactcatggttgtcctccggtgacccctctacgggagaatttactttcactgtggaccggcctgatcaagcaccttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

1402

Amino Acids

155.53

Weight (kDa)

5.87

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 58 - 593 4.2e-167 Leucine Rich Repeat Domain of unknown function (DUF7885)
B_lectin PF01453 626 - 732 7.6e-37 D-mannose binding lectin
S_locus_glycop PF00954 764 - 876 8.5e-33 S-locus glycoprotein domain
PAN_2 PF08276 897 - 963 1.3e-20 PAN-like domain
DUF3660 PF12398 1035 - 1071 7.6e-08 Receptor serine/threonine kinase
Pkinase PF00069 1082 - 1280 1.3e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1084 - 1347 7.4e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1124
AasI GACNNNNNNGTC 3 cut(s) 937, 1525, 3690
Acc36I ACCTGC 1 cut(s) 3973
Acc65I GGTACC 1 cut(s) 2514
AccB1I GGYRCC 2 cut(s) 2514, 2654
AccBSI CCGCTC 5 cut(s) 7, 1909, 2298, 2742, 2750
AccI GTMKAC 2 cut(s) 378, 859
AccII CGCG 1 cut(s) 2247
AccIII TCCGGA 2 cut(s) 1973, 2332
AclWI GGATC 4 cut(s) 753, 1566, 2489, 3705
AcoI YGGCCR 3 cut(s) 1470, 1890, 2911
AcsI RAATTY 7 cut(s) 1009, 1019, 2372, 2722, 3105, 3359, 4169
AcuI CTGAAG 3 cut(s) 414, 1658, 2015
AcyI GRCGYC 1 cut(s) 2574
AfaI GTAC 8 cut(s) 923, 1477, 1841, 1856, 2291, 2399, 2516, 2543
AfeI AGCGCT 2 cut(s) 82, 668
AflIII ACRYGT 1 cut(s) 2538
AhdI GACNNNNNGTC 1 cut(s) 244
AhlI ACTAGT 1 cut(s) 578
AjuI GAANNNNNNNTTGG 6 cut(s) 1867, 1899, 1969, 2001, 2153, 2185
AleI CACNNNNGTG 1 cut(s) 1324
Alw21I GWGCWC 3 cut(s) 119, 271, 3162
Alw44I GTGCAC 1 cut(s) 267
AlwI GGATC 4 cut(s) 753, 1566, 2489, 3705
AlwNI CAGNNNCTG 3 cut(s) 1265, 2942, 3962
Ama87I CYCGRG 3 cut(s) 3061, 3561, 4122
Aor13HI TCCGGA 2 cut(s) 1973, 2332
Aor51HI AGCGCT 2 cut(s) 82, 668
ApaI GGGCCC 1 cut(s) 2559
ApaLI GTGCAC 1 cut(s) 267
ApeKI GCWGC 7 cut(s) 66, 297, 544, 664, 2731, 3224, 4051
ApoI RAATTY 7 cut(s) 1009, 1019, 2372, 2722, 3105, 3359, 4169
ArsI GACNNNNNNTTYG 4 cut(s) 1043, 1075, 1738, 1770
Asp700I GAANNNNTTC 4 cut(s) 1019, 1954, 1981, 3105
Asp718I GGTACC 1 cut(s) 2514
AspA2I CCTAGG 1 cut(s) 3880
AspLEI GCGC 3 cut(s) 83, 234, 669
AsuC2I CCSGG 4 cut(s) 1656, 2255, 4123, 4124
AsuII TTCGAA 2 cut(s) 1023, 3109
AsuNHI GCTAGC 1 cut(s) 2348
AvaI CYCGRG 3 cut(s) 3061, 3561, 4122
AvaII GGWCC 8 cut(s) 29, 1162, 2056, 2527, 3092, 3613, 3652, 4186
AvrII CCTAGG 1 cut(s) 3880
AxyI CCTNAGG 1 cut(s) 3074
BaeGI GKGCMC 2 cut(s) 271, 2559
BalI TGGCCA 1 cut(s) 1892
BanI GGYRCC 2 cut(s) 2514, 2654
BanII GRGCYC 2 cut(s) 2332, 2559
BarI GAAGNNNNNNTAC 4 cut(s) 1350, 1382, 3146, 3178
BauI CACGAG 2 cut(s) 2188, 2809
BbsI GAAGAC 1 cut(s) 567
Bbv12I GWGCWC 3 cut(s) 119, 271, 3162
BbvI GCAGC 7 cut(s) 53, 284, 556, 676, 2718, 3236, 4063
BceAI ACGGC 4 cut(s) 1297, 2453, 2486, 2993
BciVI GTATCC 1 cut(s) 1934
BclI TGATCA 2 cut(s) 75, 4195
BcnI CCSGG 4 cut(s) 1656, 2255, 4123, 4124
BcuI ACTAGT 1 cut(s) 578
BfmI CTRYAG 2 cut(s) 1189, 3219
BfoI RGCGCY 2 cut(s) 84, 670
BfuAI ACCTGC 1 cut(s) 3973
BfuI GTATCC 1 cut(s) 1934
BlnI CCTAGG 1 cut(s) 3880
BmcAI AGTACT 1 cut(s) 923
Bme18I GGWCC 8 cut(s) 29, 1162, 2056, 2527, 3092, 3613, 3652, 4186
BmeRI GACNNNNNGTC 1 cut(s) 244
BmeT110I CYCGRG 3 cut(s) 3061, 3561, 4122
BmrI ACTGGG 1 cut(s) 3269
BmsI GCATC 8 cut(s) 543, 580, 995, 1235, 2572, 3424, 3438, 3965
BmtI GCTAGC 1 cut(s) 2352
BmuI ACTGGG 1 cut(s) 3269
BpiI GAAGAC 1 cut(s) 567
BplI GAGNNNNNCTC 2 cut(s) 3149, 3181
BpmI CTGGAG 4 cut(s) 946, 1187, 2521, 2705
Bpu14I TTCGAA 2 cut(s) 1023, 3109
BpuEI CTTGAG 7 cut(s) 144, 575, 1816, 2665, 3130, 3602, 4099
BpuMI CCSGG 4 cut(s) 1656, 2255, 4123, 4124
BsaAI YACGTR 1 cut(s) 2541
BsaHI GRCGYC 1 cut(s) 2574
BsaI GGTCTC 2 cut(s) 47, 3776
BsaJI CCNNGG 9 cut(s) 1654, 1739, 1819, 2245, 2303, 2522, 2523, 3880, 4122
BsaWI WCCGGW 6 cut(s) 1555, 1973, 2165, 2292, 2332, 4148
BsaXI ACNNNNNCTCC 6 cut(s) 2672, 2702, 2739, 2769, 2842, 2872
Bse118I RCCGGY 6 cut(s) 547, 1472, 1787, 2590, 2900, 4188
Bse1I ACTGG 7 cut(s) 108, 1152, 1670, 1926, 2221, 2506, 3264
Bse21I CCTNAGG 1 cut(s) 3074
Bse3DI GCAATG 6 cut(s) 147, 659, 773, 1221, 3187, 3768
BseAI TCCGGA 2 cut(s) 1973, 2332
BseDI CCNNGG 9 cut(s) 1654, 1739, 1819, 2245, 2303, 2522, 2523, 3880, 4122
BseMI GCAATG 6 cut(s) 147, 659, 773, 1221, 3187, 3768
BseMII CTCAG 8 cut(s) 165, 367, 1479, 1513, 1729, 1935, 3065, 3954
BseNI ACTGG 7 cut(s) 108, 1152, 1670, 1926, 2221, 2506, 3264
BseSI GKGCMC 2 cut(s) 271, 2559
BseXI GCAGC 7 cut(s) 53, 284, 556, 676, 2718, 3236, 4063
BseYI CCCAGC 1 cut(s) 62
BsgI GTGCAG 3 cut(s) 910, 1646, 4032
Bsh1236I CGCG 1 cut(s) 2247
BshNI GGYRCC 2 cut(s) 2514, 2654
BsiHKAI GWGCWC 3 cut(s) 119, 271, 3162
BsiHKCI CYCGRG 3 cut(s) 3061, 3561, 4122
BslFI GGGAC 4 cut(s) 1513, 2540, 3276, 3626
BsmBI CGTCTC 1 cut(s) 1786
BsmFI GGGAC 4 cut(s) 1513, 2540, 3276, 3626
BsmI GAATGC 2 cut(s) 426, 653
Bso31I GGTCTC 2 cut(s) 47, 3776
BsoBI CYCGRG 3 cut(s) 3061, 3561, 4122
Bsp119I TTCGAA 2 cut(s) 1023, 3109
Bsp120I GGGCCC 1 cut(s) 2555
Bsp1286I GDGCHC 5 cut(s) 119, 271, 2332, 2559, 3162
Bsp13I TCCGGA 2 cut(s) 1973, 2332
Bsp19I CCATGG 1 cut(s) 1739
BspCNI CTCAG 8 cut(s) 164, 366, 1480, 1512, 1728, 1934, 3066, 3955
BspEI TCCGGA 2 cut(s) 1973, 2332
BspFNI CGCG 1 cut(s) 2247
BspMI ACCTGC 1 cut(s) 3973
BspOI GCTAGC 1 cut(s) 2352
BspPI GGATC 4 cut(s) 753, 1566, 2489, 3705
BspT104I TTCGAA 2 cut(s) 1023, 3109
BspT107I GGYRCC 2 cut(s) 2514, 2654
BspTNI GGTCTC 2 cut(s) 47, 3776
BsrBI CCGCTC 5 cut(s) 7, 1909, 2298, 2742, 2750
BsrDI GCAATG 6 cut(s) 147, 659, 773, 1221, 3187, 3768
BsrFI RCCGGY 6 cut(s) 547, 1472, 1787, 2590, 2900, 4188
BsrI ACTGG 7 cut(s) 108, 1152, 1670, 1926, 2221, 2506, 3264
BssAI RCCGGY 6 cut(s) 547, 1472, 1787, 2590, 2900, 4188
BssECI CCNNGG 9 cut(s) 1654, 1739, 1819, 2245, 2303, 2522, 2523, 3880, 4122
BssNI GRCGYC 1 cut(s) 2574
BssSI CACGAG 2 cut(s) 2188, 2809
BssT1I CCWWGG 2 cut(s) 1739, 3880
Bst2BI CACGAG 2 cut(s) 2188, 2809
Bst4CI ACNGT 7 cut(s) 1138, 1340, 1407, 1879, 2280, 3134, 4183
Bst6I CTCTTC 3 cut(s) 1634, 3317, 3779
BstACI GRCGYC 1 cut(s) 2574
BstAPI GCANNNNNTGC 2 cut(s) 550, 983
BstBAI YACGTR 1 cut(s) 2541
BstBI TTCGAA 2 cut(s) 1023, 3109
BstDSI CCRYGG 2 cut(s) 1739, 2245
BstEII GGTNACC 1 cut(s) 4151
BstFNI CGCG 1 cut(s) 2247
BstH2I RGCGCY 2 cut(s) 84, 670
BstHHI GCGC 3 cut(s) 83, 234, 669
BstNSI RCATGY 2 cut(s) 907, 3982
BstPI GGTNACC 1 cut(s) 4151
BstSFI CTRYAG 2 cut(s) 1189, 3219
BstSLI GKGCMC 2 cut(s) 271, 2559
BstUI CGCG 1 cut(s) 2247
BstV1I GCAGC 7 cut(s) 53, 284, 556, 676, 2718, 3236, 4063
BstV2I GAAGAC 1 cut(s) 567
BstX2I RGATCY 1 cut(s) 745
BstYI RGATCY 1 cut(s) 745
Bsu36I CCTNAGG 1 cut(s) 3074
BsuI GTATCC 1 cut(s) 1934
BtgI CCRYGG 2 cut(s) 1739, 2245
BtgZI GCGATG 1 cut(s) 1212
BtsIMutI CAGTG 6 cut(s) 1403, 1663, 1723, 2513, 3139, 4179
BveI ACCTGC 1 cut(s) 3973
CaiI CAGNNNCTG 3 cut(s) 1265, 2942, 3962
CfoI GCGC 3 cut(s) 83, 234, 669
Cfr10I RCCGGY 6 cut(s) 547, 1472, 1787, 2590, 2900, 4188
Cfr42I CCGCGG 1 cut(s) 2248
Cfr9I CCCGGG 1 cut(s) 4122
CseI GACGC 4 cut(s) 118, 983, 2582, 3988
Csp6I GTAC 8 cut(s) 922, 1476, 1840, 1855, 2290, 2398, 2515, 2542
CspCI CAANNNNNGTGG 2 cut(s) 1449, 1484
CviQI GTAC 8 cut(s) 922, 1476, 1840, 1855, 2290, 2398, 2515, 2542
DrdI GACNNNNNNGTC 3 cut(s) 937, 1525, 3690
DriI GACNNNNNGTC 1 cut(s) 244
DseDI GACNNNNNNGTC 3 cut(s) 937, 1525, 3690
EaeI YGGCCR 3 cut(s) 1470, 1890, 2911
Eam1104I CTCTTC 3 cut(s) 1634, 3317, 3779
Eam1105I GACNNNNNGTC 1 cut(s) 244
EarI CTCTTC 3 cut(s) 1634, 3317, 3779
EciI GGCGGA 1 cut(s) 2760
Eco130I CCWWGG 2 cut(s) 1739, 3880
Eco147I AGGCCT 2 cut(s) 1645, 4024
Eco24I GRGCYC 2 cut(s) 2332, 2559
Eco31I GGTCTC 2 cut(s) 47, 3776
Eco32I GATATC 1 cut(s) 3663
Eco47I GGWCC 8 cut(s) 29, 1162, 2056, 2527, 3092, 3613, 3652, 4186
Eco47III AGCGCT 2 cut(s) 82, 668
Eco57I CTGAAG 3 cut(s) 414, 1658, 2015
Eco81I CCTNAGG 1 cut(s) 3074
Eco88I CYCGRG 3 cut(s) 3061, 3561, 4122
Eco91I GGTNACC 1 cut(s) 4151
EcoO109I RGGNCCY 5 cut(s) 1162, 2527, 2556, 3092, 3613
EcoO65I GGTNACC 1 cut(s) 4151
EcoRI GAATTC 2 cut(s) 3105, 3359
EcoRV GATATC 1 cut(s) 3663
EcoT14I CCWWGG 2 cut(s) 1739, 3880
EcoT22I ATGCAT 3 cut(s) 595, 988, 3980
EcoT38I GRGCYC 2 cut(s) 2332, 2559
ErhI CCWWGG 2 cut(s) 1739, 3880
Esp3I CGTCTC 1 cut(s) 1786
FalI AAGNNNNNCTT 2 cut(s) 3081, 3113
FaqI GGGAC 4 cut(s) 1513, 2540, 3276, 3626
FauI CCCGC 2 cut(s) 2318, 2546
FauNDI CATATG 2 cut(s) 1850, 3736
FbaI TGATCA 2 cut(s) 75, 4195
FblI GTMKAC 2 cut(s) 378, 859
FriOI GRGCYC 2 cut(s) 2332, 2559
GlaI GCGC 3 cut(s) 82, 233, 668
GsaI CCCAGC 1 cut(s) 66
GsuI CTGGAG 4 cut(s) 946, 1187, 2521, 2705
HaeII RGCGCY 2 cut(s) 84, 670
HgaI GACGC 4 cut(s) 118, 983, 2582, 3988
HhaI GCGC 3 cut(s) 83, 234, 669
Hin1I GRCGYC 1 cut(s) 2574
Hin6I GCGC 3 cut(s) 81, 232, 667
HinP1I GCGC 3 cut(s) 81, 232, 667
HincII GTYRAC 1 cut(s) 1066
HindII GTYRAC 1 cut(s) 1066
HindIII AAGCTT 1 cut(s) 396
Hpy166II GTNNAC 9 cut(s) 269, 379, 860, 1066, 1574, 2383, 2669, 3286, 4186
Hpy8I GTNNAC 9 cut(s) 269, 379, 860, 1066, 1574, 2383, 2669, 3286, 4186
Hpy99I CGWCG 3 cut(s) 250, 1949, 2576
HpyCH4III ACNGT 7 cut(s) 1138, 1340, 1407, 1879, 2280, 3134, 4183
HpyCH4IV ACGT 3 cut(s) 564, 2540, 2671
HpySE526I ACGT 3 cut(s) 564, 2540, 2671
Hsp92I GRCGYC 1 cut(s) 2574
HspAI GCGC 3 cut(s) 81, 232, 667
KflI GGGWCCC 1 cut(s) 2527
Kpn2I TCCGGA 2 cut(s) 1973, 2332
KpnI GGTACC 1 cut(s) 2518
KroI GCCGGC 2 cut(s) 1787, 2900
KroNI GCCGGC 2 cut(s) 1789, 2902
Ksp22I TGATCA 2 cut(s) 75, 4195
KspI CCGCGG 1 cut(s) 2248
Lsp1109I GCAGC 7 cut(s) 53, 284, 556, 676, 2718, 3236, 4063
LweI GCATC 8 cut(s) 543, 580, 995, 1235, 2572, 3424, 3438, 3965
MaeII ACGT 3 cut(s) 564, 2540, 2671
MaeIII GTNAC 5 cut(s) 725, 971, 1348, 1727, 4151
MbiI CCGCTC 5 cut(s) 7, 1909, 2298, 2742, 2750
MfeI CAATTG 4 cut(s) 223, 1392, 1461, 1600
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 5 cut(s) 119, 271, 2332, 2559, 3162
MlsI TGGCCA 1 cut(s) 1892
MluNI TGGCCA 1 cut(s) 1892
MlyI GAGTC 7 cut(s) 236, 455, 820, 925, 947, 1199, 1616
MmeI TCCRAC 4 cut(s) 2491, 2641, 3768, 3900
Mox20I TGGCCA 1 cut(s) 1892
Mph1103I ATGCAT 3 cut(s) 595, 988, 3980
MroI TCCGGA 2 cut(s) 1973, 2332
MroNI GCCGGC 2 cut(s) 1787, 2900
MroXI GAANNNNTTC 4 cut(s) 1019, 1954, 1981, 3105
MscI TGGCCA 1 cut(s) 1892
MseI TTAA 8 cut(s) 207, 402, 426, 791, 882, 1241, 2016, 3380
MslI CAYNNNNRTG 2 cut(s) 1324, 2978
Msp20I TGGCCA 1 cut(s) 1892
MspA1I CMGCKG 5 cut(s) 66, 2247, 2910, 3023, 3227
MunI CAATTG 4 cut(s) 223, 1392, 1461, 1600
Mva1269I GAATGC 2 cut(s) 426, 653
MvnI CGCG 1 cut(s) 2247
NaeI GCCGGC 2 cut(s) 1789, 2902
NciI CCSGG 4 cut(s) 1656, 2255, 4123, 4124
NcoI CCATGG 1 cut(s) 1739
NdeI CATATG 2 cut(s) 1850, 3736
NgoMIV GCCGGC 2 cut(s) 1787, 2900
NheI GCTAGC 1 cut(s) 2348
NmuCI GTSAC 4 cut(s) 725, 971, 1348, 4151
NsiI ATGCAT 3 cut(s) 595, 988, 3980
NspI RCATGY 2 cut(s) 907, 3982
NspV TTCGAA 2 cut(s) 1023, 3109
OliI CACNNNNGTG 1 cut(s) 1324
PaeI GCATGC 1 cut(s) 3982
PaeR7I CTCGAG 1 cut(s) 3561
PasI CCCWGGG 1 cut(s) 2523
PceI AGGCCT 2 cut(s) 1645, 4024
PctI GAATGC 2 cut(s) 426, 653
PdiI GCCGGC 2 cut(s) 1789, 2902
PdmI GAANNNNTTC 4 cut(s) 1019, 1954, 1981, 3105
PfoI TCCNGGA 2 cut(s) 1164, 2253
PleI GAGTC 7 cut(s) 236, 454, 819, 925, 946, 1199, 1616
PpsI GAGTC 7 cut(s) 236, 454, 819, 925, 946, 1199, 1616
Ppu21I YACGTR 1 cut(s) 2541
PpuMI RGGWCCY 4 cut(s) 1162, 2527, 3092, 3613
PsiI TTATAA 1 cut(s) 1124
Psp5II RGGWCCY 4 cut(s) 1162, 2527, 3092, 3613
PspEI GGTNACC 1 cut(s) 4151
PspFI CCCAGC 1 cut(s) 62
PspOMI GGGCCC 1 cut(s) 2555
PspPPI RGGWCCY 4 cut(s) 1162, 2527, 3092, 3613
PspXI VCTCGAGB 1 cut(s) 3561
PstNI CAGNNNCTG 3 cut(s) 1265, 2942, 3962
PsuI RGATCY 1 cut(s) 745
PvuII CAGCTG 2 cut(s) 66, 3023
RsaI GTAC 8 cut(s) 923, 1477, 1841, 1856, 2291, 2399, 2516, 2543
RsaNI GTAC 8 cut(s) 922, 1476, 1840, 1855, 2290, 2398, 2515, 2542
RseI CAYNNNNRTG 2 cut(s) 1324, 2978
SacII CCGCGG 1 cut(s) 2248
SaqAI TTAA 8 cut(s) 207, 402, 426, 791, 882, 1241, 2016, 3380
ScaI AGTACT 1 cut(s) 923
SchI GAGTC 7 cut(s) 236, 455, 820, 925, 947, 1199, 1616
SduI GDGCHC 5 cut(s) 119, 271, 2332, 2559, 3162
SfaNI GCATC 8 cut(s) 543, 580, 995, 1235, 2572, 3424, 3438, 3965
SfcI CTRYAG 2 cut(s) 1189, 3219
Sfr274I CTCGAG 1 cut(s) 3561
Sfr303I CCGCGG 1 cut(s) 2248
SfuI TTCGAA 2 cut(s) 1023, 3109
SgrBI CCGCGG 1 cut(s) 2248
SinI GGWCC 8 cut(s) 29, 1162, 2056, 2527, 3092, 3613, 3652, 4186
SlaI CTCGAG 1 cut(s) 3561
SmaI CCCGGG 1 cut(s) 4124
SmiMI CAYNNNNRTG 2 cut(s) 1324, 2978
SmlI CTYRAG 8 cut(s) 159, 554, 1831, 2644, 3145, 3561, 3617, 4078
SmoI CTYRAG 8 cut(s) 159, 554, 1831, 2644, 3145, 3561, 3617, 4078
SpeI ACTAGT 1 cut(s) 578
SphI GCATGC 1 cut(s) 3982
SseBI AGGCCT 2 cut(s) 1645, 4024
StuI AGGCCT 2 cut(s) 1645, 4024
StyI CCWWGG 2 cut(s) 1739, 3880
TaaI ACNGT 7 cut(s) 1138, 1340, 1407, 1879, 2280, 3134, 4183
TaiI ACGT 3 cut(s) 567, 2543, 2674
TaqII GACCGA 1 cut(s) 3680
TatI WGTACW 2 cut(s) 921, 2397
TauI GCSGC 6 cut(s) 10, 2301, 2493, 2745, 2913, 3230
Tru1I TTAA 8 cut(s) 207, 402, 426, 791, 882, 1241, 2016, 3380
Tru9I TTAA 8 cut(s) 207, 402, 426, 791, 882, 1241, 2016, 3380
TscAI CASTG 6 cut(s) 1410, 1670, 1723, 2513, 3139, 4186
TseFI GTSAC 4 cut(s) 725, 971, 1348, 4151
TseI GCWGC 7 cut(s) 66, 297, 544, 664, 2731, 3224, 4051
Tsp45I GTSAC 4 cut(s) 725, 971, 1348, 4151
TspGWI ACGGA 4 cut(s) 184, 1962, 2693, 3248
TspMI CCCGGG 1 cut(s) 4122
TspRI CASTG 6 cut(s) 1410, 1670, 1723, 2513, 3139, 4186
VneI GTGCAC 1 cut(s) 267
VpaK11BI GGWCC 8 cut(s) 29, 1162, 2056, 2527, 3092, 3613, 3652, 4186
XapI RAATTY 7 cut(s) 1009, 1019, 2372, 2722, 3105, 3359, 4169
XbaI TCTAGA 1 cut(s) 934
XceI RCATGY 2 cut(s) 907, 3982
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XhoI CTCGAG 1 cut(s) 3561
XmaI CCCGGG 1 cut(s) 4122
XmaJI CCTAGG 1 cut(s) 3880
XmiI GTMKAC 2 cut(s) 378, 859
XmnI GAANNNNTTC 4 cut(s) 1019, 1954, 1981, 3105
ZrmI AGTACT 1 cut(s) 923
Zsp2I ATGCAT 3 cut(s) 595, 988, 3980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.