Rmu_sc0000751.1_g000031

importin subunit beta-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000751.1
OG0016981
Physical Location & Seq
Reverse (-)
73416 .. 78747
5332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000751.1_g000031.1.cds

Sequence Viewer

Length: 1305 bp
atggaagctactgagttgtacaagggaatggataaaaatactaaagctgctaatgacattattaagagtatgaaaaaaattgcagaaaaggtaattgatggagtgatctgtaagttggctgaattggtacgtccttctgtgcataagattcttgttttgattgaacctctattgattgatgacaactattatgtacgtgttgaggggagactcactcaaaatcccagctattataatcttcagggtattcaaaggagtttttcagattacctctcagagcttattgaaaatacattgactgacttggagagaagcaaagaagatgttgaggaatcacaaatgaggactgctgcatttggagcattgaatgaagttgtaaagtgctgcatcaatgagattgctccaatggtgatgcacttagttccaattattatgaatgaactccaccagactcttgaaggacagaggccttcaattgatgaaagagacaagcaaaatgaactgcaaggccttctttgtggttgcttgcaggtcatcatacagaagcttagggtcacacagattgtccacattaatagggctgcaaacatttgtcgtgagcactgcacagcaaatgctacagtgggagttgattactttttgcaagctgcaactctacaagaattggtacaagtttccagtgatcaaatttctctatataagctcttggttgacatcatgcttcttagtcttgggtgccttatgaaggctttggctttgcttcttggtgaatattttgaaatgctgcatgctgcacccattgcttccttgacagttgtaaaatttgtgaggagcaccataacaatagtgatgatacaattggtgattaatgtcatgaacccatcgtttctacttgtgaagaggatcattgctacagctccacttgctgctttccagactttagatgacaactatacttctattgcgaaagttggtgtttacaatgctcatgaagaagttgagatccaacgttgtgggatagcttcaagcctgagaggtttgcaaatggtggagaagagccatacaagctcatgccatttggacaaggaagaagggcttggcctggcatgggcttggcctggctttgggatcattgattcaatgtttcgattgggaaaaagcaagcacatgggctacaactacaccaatgtggtgcagtccccatatggggatcactggcgcaacctacgccgcattggcactaccgaaatctactctcccactgggctcaacatgttctcgagcatcaggaaggacgaagtctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000060 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000819 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007079 GO:0007080 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0008092 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009987 GO:0010494 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0019894 GO:0019899 GO:0019904 GO:0022402 GO:0022607 GO:0023052 GO:0030953 GO:0031072 GO:0031090 GO:0031122 GO:0031291 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031984 GO:0032991 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0035556 GO:0035770 GO:0036464 GO:0040001 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046907 GO:0048285 GO:0050000 GO:0050789 GO:0050794 GO:0050896 GO:0051169 GO:0051170 GO:0051179 GO:0051225 GO:0051234 GO:0051276 GO:0051293 GO:0051303 GO:0051305 GO:0051310 GO:0051640 GO:0051641 GO:0051649 GO:0051653 GO:0051656 GO:0051716 GO:0051879 GO:0065007 GO:0070013 GO:0070727 GO:0070925 GO:0071702 GO:0071705 GO:0071782 GO:0071840 GO:0072594 GO:0090307 GO:0097435 GO:0098813 GO:0098827 GO:0140014 GO:1902850 GO:1903047 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
HEAT HEAT_EZ IBN_N

Protein Analysis

434

Amino Acids

48.76

Weight (kDa)

6.03

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 234
Acc36I ACCTGC 1 cut(s) 520
AccB1I GGYRCC 1 cut(s) 735
AciI CCGC 1 cut(s) 1231
AclI AACGTT 1 cut(s) 1009
AclWI GGATC 4 cut(s) 911, 997, 1136, 1218
AcsI RAATTY 2 cut(s) 687, 821
AcuI CTGAAG 1 cut(s) 224
AfaI GTAC 4 cut(s) 20, 129, 195, 669
AfiI CCNNNNNNNGG 5 cut(s) 745, 1108, 1124, 1206, 1207
AflIII ACRYGT 2 cut(s) 196, 1272
AgsI TTSAA 9 cut(s) 164, 251, 287, 367, 458, 474, 779, 1026, 1140
AjnI CCWGG 2 cut(s) 1101, 1117
AjuI GAANNNNNNNTTGG 2 cut(s) 1080, 1112
AleI CACNNNNGTG 1 cut(s) 1187
Alw21I GWGCWC 2 cut(s) 603, 836
Alw26I GTCTC 2 cut(s) 202, 480
AlwI GGATC 4 cut(s) 911, 997, 1136, 1218
Ama87I CYCGRG 1 cut(s) 1279
AoxI GGCC 4 cut(s) 467, 508, 1099, 1115
ApeKI GCWGC 8 cut(s) 47, 350, 384, 581, 647, 784, 791, 926
ApoI RAATTY 2 cut(s) 687, 821
AseI ATTAAT 2 cut(s) 573, 867
AspLEI GCGC 1 cut(s) 1221
AsuHPI GGTGA 3 cut(s) 421, 779, 874
AvaI CYCGRG 1 cut(s) 1279
BanI GGYRCC 1 cut(s) 735
BanII GRGCYC 1 cut(s) 1269
Bbv12I GWGCWC 2 cut(s) 603, 836
BbvI GCAGC 8 cut(s) 34, 337, 371, 568, 634, 771, 778, 913
BccI CCATC 2 cut(s) 92, 889
BciT130I CCWGG 2 cut(s) 1103, 1119
BclI TGATCA 1 cut(s) 682
BcoDI GTCTC 2 cut(s) 202, 480
BfaI CTAG 1 cut(s) 1303
BfmI CTRYAG 2 cut(s) 618, 912
BfuAI ACCTGC 1 cut(s) 520
BglI GCCNNNNNGGC 1 cut(s) 1236
BisI GCNGC 9 cut(s) 48, 351, 385, 582, 648, 785, 792, 927, 1231
BlsI GCNGC 9 cut(s) 49, 352, 386, 583, 649, 786, 793, 928, 1232
Bme1390I CCNGG 2 cut(s) 1103, 1119
BmeT110I CYCGRG 1 cut(s) 1279
BmiI GGNNCC 1 cut(s) 737
BmrFI CCNGG 2 cut(s) 1103, 1119
BmrI ACTGGG 1 cut(s) 1272
BmsI GCATC 3 cut(s) 396, 402, 1293
BmuI ACTGGG 1 cut(s) 1272
BplI GAGNNNNNCTC 2 cut(s) 199, 231
Bpu10I CCTNAGC 1 cut(s) 548
BsaAI YACGTR 1 cut(s) 197
Bsc4I CCNNNNNNNGG 5 cut(s) 745, 1108, 1124, 1206, 1207
Bse1I ACTGG 3 cut(s) 678, 1220, 1267
Bse3DI GCAATG 2 cut(s) 798, 906
BseBI CCWGG 2 cut(s) 1103, 1119
BseLI CCNNNNNNNGG 5 cut(s) 745, 1108, 1124, 1206, 1207
BseMI GCAATG 2 cut(s) 798, 906
BseMII CTCAG 2 cut(s) 288, 1022
BseNI ACTGG 3 cut(s) 678, 1220, 1267
BseRI GAGGAG 1 cut(s) 844
BseXI GCAGC 8 cut(s) 34, 337, 371, 568, 634, 771, 778, 913
BseYI CCCAGC 1 cut(s) 224
BsgI GTGCAG 3 cut(s) 589, 777, 1214
BshFI GGCC 4 cut(s) 469, 510, 1101, 1117
BshNI GGYRCC 1 cut(s) 735
BsiHKAI GWGCWC 2 cut(s) 603, 836
BsiHKCI CYCGRG 1 cut(s) 1279
BslFI GGGAC 1 cut(s) 1183
BslI CCNNNNNNNGG 5 cut(s) 745, 1108, 1124, 1206, 1207
BsmAI GTCTC 2 cut(s) 202, 480
BsmFI GGGAC 1 cut(s) 1183
BsnI GGCC 4 cut(s) 469, 510, 1101, 1117
BsoBI CYCGRG 1 cut(s) 1279
Bsp1286I GDGCHC 3 cut(s) 603, 836, 1269
Bsp1407I TGTACA 1 cut(s) 18
Bsp143I GATC 6 cut(s) 105, 682, 903, 1002, 1128, 1210
BspACI CCGC 1 cut(s) 1231
BspANI GGCC 4 cut(s) 469, 510, 1101, 1117
BspCNI CTCAG 3 cut(s) 4, 287, 1023
BspHI TCATGA 2 cut(s) 873, 988
BspLI GGNNCC 1 cut(s) 737
BspMI ACCTGC 1 cut(s) 520
BspPI GGATC 4 cut(s) 911, 997, 1136, 1218
BspQI GCTCTTC 1 cut(s) 1049
BspT107I GGYRCC 1 cut(s) 735
BsrDI GCAATG 2 cut(s) 798, 906
BsrGI TGTACA 1 cut(s) 18
BsrI ACTGG 3 cut(s) 678, 1220, 1267
BssMI GATC 6 cut(s) 105, 682, 903, 1002, 1128, 1210
Bst2UI CCWGG 2 cut(s) 1103, 1119
Bst4CI ACNGT 2 cut(s) 622, 814
Bst6I CTCTTC 2 cut(s) 893, 1049
BstAPI GCANNNNNTGC 1 cut(s) 800
BstAUI TGTACA 1 cut(s) 18
BstBAI YACGTR 1 cut(s) 197
BstC8I GCNNGC 4 cut(s) 527, 645, 789, 1163
BstDEI CTNAG 6 cut(s) 12, 274, 418, 548, 725, 1031
BstENI CCTNNNNNAGG 1 cut(s) 743
BstHHI GCGC 1 cut(s) 1221
BstKTI GATC 6 cut(s) 108, 685, 906, 1005, 1131, 1213
BstMAI GTCTC 2 cut(s) 202, 480
BstMBI GATC 6 cut(s) 105, 682, 903, 1002, 1128, 1210
BstMWI GCNNNNNNNGC 6 cut(s) 359, 800, 923, 1065, 1227, 1236
BstNI CCWGG 2 cut(s) 1103, 1119
BstNSI RCATGY 2 cut(s) 791, 1276
BstSCI CCNGG 2 cut(s) 1101, 1117
BstSFI CTRYAG 2 cut(s) 618, 912
BstV1I GCAGC 8 cut(s) 34, 337, 371, 568, 634, 771, 778, 913
BstX2I RGATCY 1 cut(s) 1002
BstXI CCANNNNNNTGG 1 cut(s) 1013
BstYI RGATCY 1 cut(s) 1002
BsuRI GGCC 4 cut(s) 469, 510, 1101, 1117
BtsI GCAGTG 1 cut(s) 601
BtsIMutI CAGTG 5 cut(s) 601, 627, 685, 1213, 1260
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 4 cut(s) 527, 645, 789, 1163
CciI TCATGA 2 cut(s) 873, 988
CfoI GCGC 1 cut(s) 1221
Csp6I GTAC 4 cut(s) 19, 128, 194, 668
CviAII CATG 8 cut(s) 718, 788, 874, 989, 1071, 1107, 1168, 1273
CviQI GTAC 4 cut(s) 19, 128, 194, 668
DdeI CTNAG 6 cut(s) 12, 274, 418, 548, 725, 1031
DpnI GATC 6 cut(s) 107, 684, 905, 1004, 1130, 1212
DpnII GATC 6 cut(s) 105, 682, 903, 1002, 1128, 1210
Eam1104I CTCTTC 2 cut(s) 893, 1049
EarI CTCTTC 2 cut(s) 893, 1049
Eco147I AGGCCT 2 cut(s) 469, 510
Eco24I GRGCYC 1 cut(s) 1269
Eco57I CTGAAG 1 cut(s) 224
Eco88I CYCGRG 1 cut(s) 1279
EcoNI CCTNNNNNAGG 1 cut(s) 743
EcoRII CCWGG 2 cut(s) 1101, 1117
EcoT38I GRGCYC 1 cut(s) 1269
FaeI CATG 8 cut(s) 721, 791, 877, 992, 1074, 1110, 1171, 1276
FalI AAGNNNNNCTT 4 cut(s) 498, 530, 1080, 1112
FaqI GGGAC 1 cut(s) 1183
FatI CATG 8 cut(s) 717, 787, 873, 988, 1070, 1106, 1167, 1272
FauNDI CATATG 1 cut(s) 1204
FbaI TGATCA 1 cut(s) 682
Fnu4HI GCNGC 9 cut(s) 48, 351, 385, 582, 648, 785, 792, 927, 1231
FriOI GRGCYC 1 cut(s) 1269
Fsp4HI GCNGC 9 cut(s) 48, 351, 385, 582, 648, 785, 792, 927, 1231
FspBI CTAG 1 cut(s) 1303
GlaI GCGC 1 cut(s) 1220
GluI GCNGC 9 cut(s) 48, 351, 385, 582, 648, 785, 792, 927, 1231
GsaI CCCAGC 1 cut(s) 228
HaeIII GGCC 4 cut(s) 469, 510, 1101, 1117
HhaI GCGC 1 cut(s) 1221
Hin1II CATG 8 cut(s) 721, 791, 877, 992, 1074, 1110, 1171, 1276
Hin6I GCGC 1 cut(s) 1219
HinP1I GCGC 1 cut(s) 1219
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HindIII AAGCTT 1 cut(s) 545
HinfI GANTC 5 cut(s) 148, 210, 332, 451, 1136
HphI GGTGA 3 cut(s) 421, 779, 874
Hpy166II GTNNAC 3 cut(s) 568, 712, 979
Hpy188I TCNGA 2 cut(s) 265, 277
Hpy188III TCNNGA 7 cut(s) 455, 596, 874, 934, 989, 1279, 1288
Hpy8I GTNNAC 3 cut(s) 568, 712, 979
HpyAV CCTTC 7 cut(s) 144, 452, 480, 521, 739, 1085, 1285
HpyCH4III ACNGT 2 cut(s) 622, 814
HpyCH4IV ACGT 3 cut(s) 130, 196, 1009
HpyF10VI GCNNNNNNNGC 6 cut(s) 359, 800, 923, 1065, 1227, 1236
HpyF3I CTNAG 6 cut(s) 12, 274, 418, 548, 725, 1031
HpySE526I ACGT 3 cut(s) 130, 196, 1009
Hsp92II CATG 8 cut(s) 721, 791, 877, 992, 1074, 1110, 1171, 1276
HspAI GCGC 1 cut(s) 1219
Ksp22I TGATCA 1 cut(s) 682
Kzo9I GATC 6 cut(s) 105, 682, 903, 1002, 1128, 1210
LguI GCTCTTC 1 cut(s) 1049
LmnI GCTCC 4 cut(s) 359, 406, 831, 922
Lsp1109I GCAGC 8 cut(s) 34, 337, 371, 568, 634, 771, 778, 913
LweI GCATC 3 cut(s) 396, 402, 1293
MaeI CTAG 1 cut(s) 1303
MaeII ACGT 3 cut(s) 130, 196, 1009
MaeIII GTNAC 1 cut(s) 553
MalI GATC 6 cut(s) 107, 684, 905, 1004, 1130, 1212
MboI GATC 6 cut(s) 105, 682, 903, 1002, 1128, 1210
MboII GAAGA 6 cut(s) 230, 332, 910, 1004, 1066, 1100
MfeI CAATTG 2 cut(s) 474, 857
MflI RGATCY 1 cut(s) 1002
MhlI GDGCHC 3 cut(s) 603, 836, 1269
MluCI AATT 9 cut(s) 78, 93, 122, 426, 474, 662, 687, 821, 857
MlyI GAGTC 2 cut(s) 204, 445
MmeI TCCRAC 1 cut(s) 1030
MnlI CCTC 9 cut(s) 177, 196, 281, 322, 336, 459, 822, 894, 1028
MseI TTAA 3 cut(s) 63, 573, 867
MslI CAYNNNNRTG 1 cut(s) 1187
MspR9I CCNGG 2 cut(s) 1103, 1119
MunI CAATTG 2 cut(s) 474, 857
MvaI CCWGG 2 cut(s) 1103, 1119
MwoI GCNNNNNNNGC 6 cut(s) 359, 800, 923, 1065, 1227, 1236
NdeI CATATG 1 cut(s) 1204
NdeII GATC 6 cut(s) 105, 682, 903, 1002, 1128, 1210
NlaIII CATG 8 cut(s) 721, 791, 877, 992, 1074, 1110, 1171, 1276
NlaIV GGNNCC 1 cut(s) 737
NmuCI GTSAC 1 cut(s) 553
NspI RCATGY 2 cut(s) 791, 1276
OliI CACNNNNGTG 1 cut(s) 1187
PaeI GCATGC 1 cut(s) 791
PaeR7I CTCGAG 1 cut(s) 1279
PagI TCATGA 2 cut(s) 873, 988
PceI AGGCCT 2 cut(s) 469, 510
PciI ACATGT 1 cut(s) 1272
PciSI GCTCTTC 1 cut(s) 1049
PfeI GAWTC 3 cut(s) 148, 332, 1136
PflFI GACNNNGTC 1 cut(s) 1298
PkrI GCNGC 9 cut(s) 49, 352, 386, 583, 649, 786, 793, 928, 1232
PleI GAGTC 2 cut(s) 204, 445
PpsI GAGTC 2 cut(s) 204, 445
Ppu21I YACGTR 1 cut(s) 197
PscI ACATGT 1 cut(s) 1272
PshBI ATTAAT 2 cut(s) 573, 867
PsiI TTATAA 1 cut(s) 234
Psp1406I AACGTT 1 cut(s) 1009
Psp6I CCWGG 2 cut(s) 1101, 1117
PspFI CCCAGC 1 cut(s) 224
PspGI CCWGG 2 cut(s) 1101, 1117
PspN4I GGNNCC 1 cut(s) 737
PsuI RGATCY 1 cut(s) 1002
PsyI GACNNNGTC 1 cut(s) 1298
RsaI GTAC 4 cut(s) 20, 129, 195, 669
RsaNI GTAC 4 cut(s) 19, 128, 194, 668
RseI CAYNNNNRTG 1 cut(s) 1187
SapI GCTCTTC 1 cut(s) 1049
SaqAI TTAA 3 cut(s) 63, 573, 867
SatI GCNGC 9 cut(s) 48, 351, 385, 582, 648, 785, 792, 927, 1231
Sau3AI GATC 6 cut(s) 105, 682, 903, 1002, 1128, 1210
SchI GAGTC 2 cut(s) 204, 445
ScrFI CCNGG 2 cut(s) 1103, 1119
SduI GDGCHC 3 cut(s) 603, 836, 1269
SfaNI GCATC 3 cut(s) 396, 402, 1293
SfcI CTRYAG 2 cut(s) 618, 912
Sfr274I CTCGAG 1 cut(s) 1279
SlaI CTCGAG 1 cut(s) 1279
SmiMI CAYNNNNRTG 1 cut(s) 1187
SmlI CTYRAG 1 cut(s) 1279
SmoI CTYRAG 1 cut(s) 1279
SphI GCATGC 1 cut(s) 791
Sse9I AATT 9 cut(s) 78, 93, 122, 426, 474, 662, 687, 821, 857
SseBI AGGCCT 2 cut(s) 469, 510
SsiI CCGC 1 cut(s) 1231
SspI AATATT 1 cut(s) 773
SspMI CTAG 1 cut(s) 1303
StuI AGGCCT 2 cut(s) 469, 510
StyD4I CCNGG 2 cut(s) 1101, 1117
TaaI ACNGT 2 cut(s) 622, 814
TaiI ACGT 3 cut(s) 133, 199, 1012
TaqI TCGA 2 cut(s) 1147, 1280
TasI AATT 9 cut(s) 78, 93, 122, 426, 474, 662, 687, 821, 857
TatI WGTACW 1 cut(s) 18
TauI GCSGC 1 cut(s) 1233
TfiI GAWTC 3 cut(s) 148, 332, 1136
Tru1I TTAA 3 cut(s) 63, 573, 867
Tru9I TTAA 3 cut(s) 63, 573, 867
TscAI CASTG 5 cut(s) 608, 627, 685, 1220, 1267
TseFI GTSAC 1 cut(s) 553
TseI GCWGC 8 cut(s) 47, 350, 384, 581, 647, 784, 791, 926
Tsp45I GTSAC 1 cut(s) 553
TspDTI ATGAA 9 cut(s) 86, 384, 449, 453, 495, 513, 758, 890, 1005
TspRI CASTG 5 cut(s) 608, 627, 685, 1220, 1267
Tth111I GACNNNGTC 1 cut(s) 1298
VspI ATTAAT 2 cut(s) 573, 867
XagI CCTNNNNNAGG 1 cut(s) 743
XapI RAATTY 2 cut(s) 687, 821
XceI RCATGY 2 cut(s) 791, 1276
XhoI CTCGAG 1 cut(s) 1279
XspI CTAG 1 cut(s) 1303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.