Rmu_sc0000911.1_g000012

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000911.1
OG0017768
Physical Location & Seq
Forward (+)
50614 .. 51438
825 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 825 bp
atggtgaacattgaggtcactgttatttccagagacaccattaaaccatcaactgcttcctcttcgcctcgccttctacaaccttacaagcttaacatttgcgatcagctcgttccctacacttatgtcccaatgattttcttctaccccattactgtccccagcttcaatctccttcaagccctaatccacttgagaaactccctctccgaggccctcactctgtactacccattgtccggaaggataaaaaacaacctctatgtacatgacttcgacgcgggtgttccctaccttgaagctcaagtcaattgtcccatgctcgagtttcttaagcttcgagaaaccgaatggctaaaccacctcgtaccattccatccatttctcaaggaaatggattctgagctactgcccctcaccgcatttcaagccaacatcttcgatggtggaatagcagtgggcatttccttttctcacaagctcattgatggggaaacagccaatgcttttctcaagacttgggctgctatttttcgtggctgtcgccaagaaatcatataccctaatcttgtccaagcctcattagtttttccaccacgagataacttgcctaaaaggtatgtaactatcatggagaggttgtggttcggccagaaaaaggattatgttacaaggagatttctgtttgatgctaaagccatatccgcacttcaagtcgaagcaaagagcgagctcgtgcccaaaccatcacgtattgaggcacttacatgttttctctggaaacatcaaatggctgcctctcgggcgttatcatcaggtaattaa

Protein Analysis

274

Amino Acids

31.29

Weight (kDa)

8.8

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 269 1.2e-43 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 281
AccIII TCCGGA 1 cut(s) 239
AciI CCGC 3 cut(s) 281, 420, 705
AcoI YGGCCR 1 cut(s) 649
AfaI GTAC 3 cut(s) 227, 267, 369
AfiI CCNNNNNNNGG 3 cut(s) 211, 239, 658
AflII CTTAAG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 767
AgsI TTSAA 5 cut(s) 169, 179, 299, 428, 713
AluBI AGCT 8 cut(s) 91, 109, 165, 302, 337, 406, 481, 733
AluI AGCT 8 cut(s) 91, 109, 165, 302, 337, 406, 481, 733
Alw21I GWGCWC 1 cut(s) 735
Alw26I GTCTC 1 cut(s) 27
Ama87I CYCGRG 2 cut(s) 323, 801
Aor13HI TCCGGA 1 cut(s) 239
AoxI GGCC 2 cut(s) 213, 649
ApeKI GCWGC 2 cut(s) 524, 794
AspS9I GGNCC 1 cut(s) 214
AsuHPI GGTGA 2 cut(s) 16, 409
AvaI CYCGRG 2 cut(s) 323, 801
BaeGI GKGCMC 1 cut(s) 741
BanII GRGCYC 1 cut(s) 735
BauI CACGAG 2 cut(s) 597, 734
Bbv12I GWGCWC 1 cut(s) 735
BbvI GCAGC 2 cut(s) 511, 781
BccI CCATC 5 cut(s) 55, 384, 437, 482, 754
BcoDI GTCTC 1 cut(s) 27
BfrI CTTAAG 1 cut(s) 332
BglI GCCNNNNNGGC 1 cut(s) 803
BisI GCNGC 2 cut(s) 525, 795
BlsI GCNGC 2 cut(s) 526, 796
BmeT110I CYCGRG 2 cut(s) 323, 801
BmgT120I GGNCC 1 cut(s) 214
BmsI GCATC 1 cut(s) 679
BpuEI CTTGAG 4 cut(s) 214, 288, 371, 497
BsaAI YACGTR 1 cut(s) 752
BsaJI CCNNGG 1 cut(s) 210
BsaWI WCCGGW 1 cut(s) 239
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 626, 656
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 239, 658
BseAI TCCGGA 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 1 cut(s) 376
BseLI CCNNNNNNNGG 3 cut(s) 211, 239, 658
BseMII CTCAG 1 cut(s) 393
BseSI GKGCMC 1 cut(s) 741
BseXI GCAGC 2 cut(s) 511, 781
BseYI CCCAGC 1 cut(s) 161
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 2 cut(s) 215, 651
BsiHKAI GWGCWC 1 cut(s) 735
BsiHKCI CYCGRG 2 cut(s) 323, 801
BsiSI CCGG 1 cut(s) 240
BslFI GGGAC 3 cut(s) 113, 143, 300
BslI CCNNNNNNNGG 3 cut(s) 211, 239, 658
BsmAI GTCTC 1 cut(s) 27
BsmFI GGGAC 3 cut(s) 113, 143, 300
BsnI GGCC 2 cut(s) 215, 651
BsoBI CYCGRG 2 cut(s) 323, 801
Bsp1286I GDGCHC 2 cut(s) 735, 741
Bsp13I TCCGGA 1 cut(s) 239
Bsp1407I TGTACA 1 cut(s) 265
Bsp143I GATC 1 cut(s) 103
BspACI CCGC 3 cut(s) 281, 420, 705
BspANI GGCC 2 cut(s) 215, 651
BspCNI CTCAG 1 cut(s) 394
BspEI TCCGGA 1 cut(s) 239
BspFNI CGCG 1 cut(s) 281
BspTI CTTAAG 1 cut(s) 332
BsrGI TGTACA 1 cut(s) 265
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 1 cut(s) 103
BssSI CACGAG 2 cut(s) 597, 734
Bst2BI CACGAG 2 cut(s) 597, 734
Bst4CI ACNGT 2 cut(s) 22, 157
Bst6I CTCTTC 1 cut(s) 67
BstAFI CTTAAG 1 cut(s) 332
BstAUI TGTACA 1 cut(s) 265
BstBAI YACGTR 1 cut(s) 752
BstC8I GCNNGC 1 cut(s) 731
BstDEI CTNAG 1 cut(s) 402
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 1 cut(s) 376
BstFNI CGCG 1 cut(s) 281
BstKTI GATC 1 cut(s) 106
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 1 cut(s) 103
BstMWI GCNNNNNNNGC 3 cut(s) 428, 704, 803
BstNSI RCATGY 1 cut(s) 771
BstSLI GKGCMC 1 cut(s) 741
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 2 cut(s) 511, 781
BsuRI GGCC 2 cut(s) 215, 651
BtsCI GGATG 1 cut(s) 376
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 2 cut(s) 18, 462
Cac8I GCNNGC 1 cut(s) 731
Cfr13I GGNCC 1 cut(s) 214
CseI GACGC 1 cut(s) 287
Csp6I GTAC 3 cut(s) 226, 266, 368
CviAII CATG 4 cut(s) 269, 319, 631, 768
CviQI GTAC 3 cut(s) 226, 266, 368
DdeI CTNAG 1 cut(s) 402
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 649
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
Ecl136II GAGCTC 1 cut(s) 733
Eco24I GRGCYC 1 cut(s) 735
Eco53kI GAGCTC 1 cut(s) 733
Eco88I CYCGRG 2 cut(s) 323, 801
EcoICRI GAGCTC 1 cut(s) 733
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoO109I RGGNCCY 1 cut(s) 214
EcoT38I GRGCYC 1 cut(s) 735
FaeI CATG 4 cut(s) 272, 322, 634, 771
FaqI GGGAC 3 cut(s) 113, 143, 300
FatI CATG 4 cut(s) 268, 318, 630, 767
FauI CCCGC 1 cut(s) 274
Fnu4HI GCNGC 2 cut(s) 525, 795
FokI GGATG 1 cut(s) 363
FriOI GRGCYC 1 cut(s) 735
Fsp4HI GCNGC 2 cut(s) 525, 795
GluI GCNGC 2 cut(s) 525, 795
GsaI CCCAGC 1 cut(s) 165
HaeIII GGCC 2 cut(s) 215, 651
HapII CCGG 1 cut(s) 240
HgaI GACGC 1 cut(s) 287
Hin1II CATG 4 cut(s) 272, 322, 634, 771
HindIII AAGCTT 2 cut(s) 89, 335
HinfI GANTC 1 cut(s) 398
HpaII CCGG 1 cut(s) 240
HphI GGTGA 2 cut(s) 16, 409
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 2 cut(s) 211, 403
Hpy188III TCNNGA 5 cut(s) 30, 240, 341, 514, 778
Hpy8I GTNNAC 1 cut(s) 7
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 3 cut(s) 83, 185, 237
HpyCH4III ACNGT 2 cut(s) 22, 157
HpyCH4IV ACGT 1 cut(s) 751
HpyF10VI GCNNNNNNNGC 3 cut(s) 428, 704, 803
HpyF3I CTNAG 1 cut(s) 402
HpySE526I ACGT 1 cut(s) 751
Hsp92II CATG 4 cut(s) 272, 322, 634, 771
Kpn2I TCCGGA 1 cut(s) 239
Kzo9I GATC 1 cut(s) 103
LpnPI CCDG 6 cut(s) 43, 175, 253, 665, 763, 801
Lsp1109I GCAGC 2 cut(s) 511, 781
LweI GCATC 1 cut(s) 679
MaeII ACGT 1 cut(s) 751
MaeIII GTNAC 3 cut(s) 16, 622, 667
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 3 cut(s) 54, 133, 430
MfeI CAATTG 1 cut(s) 310
MhlI GDGCHC 2 cut(s) 735, 741
MluCI AATT 2 cut(s) 310, 820
MroI TCCGGA 1 cut(s) 239
MseI TTAA 4 cut(s) 42, 93, 333, 823
MslI CAYNNNNRTG 1 cut(s) 766
MspCI CTTAAG 1 cut(s) 332
MspI CCGG 1 cut(s) 240
MunI CAATTG 1 cut(s) 310
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 3 cut(s) 428, 704, 803
NdeII GATC 1 cut(s) 103
NlaIII CATG 4 cut(s) 272, 322, 634, 771
NmuCI GTSAC 1 cut(s) 16
NspI RCATGY 1 cut(s) 771
PaeR7I CTCGAG 1 cut(s) 323
PciI ACATGT 1 cut(s) 767
PfeI GAWTC 1 cut(s) 398
PkrI GCNGC 2 cut(s) 526, 796
Ppu21I YACGTR 1 cut(s) 752
PscI ACATGT 1 cut(s) 767
Psp124BI GAGCTC 1 cut(s) 735
PspFI CCCAGC 1 cut(s) 161
PspPI GGNCC 1 cut(s) 214
PspXI VCTCGAGB 1 cut(s) 323
RsaI GTAC 3 cut(s) 227, 267, 369
RsaNI GTAC 3 cut(s) 226, 266, 368
RseI CAYNNNNRTG 1 cut(s) 766
SacI GAGCTC 1 cut(s) 735
SaqAI TTAA 4 cut(s) 42, 93, 333, 823
SatI GCNGC 2 cut(s) 525, 795
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 1 cut(s) 214
SduI GDGCHC 2 cut(s) 735, 741
SfaNI GCATC 1 cut(s) 679
Sfr274I CTCGAG 1 cut(s) 323
SlaI CTCGAG 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 766
SmlI CTYRAG 6 cut(s) 193, 303, 323, 332, 386, 512
SmoI CTYRAG 6 cut(s) 193, 303, 323, 332, 386, 512
Sse9I AATT 2 cut(s) 310, 820
SsiI CCGC 3 cut(s) 281, 420, 705
SstI GAGCTC 1 cut(s) 735
TaaI ACNGT 2 cut(s) 22, 157
TaiI ACGT 1 cut(s) 754
TaqI TCGA 5 cut(s) 276, 324, 340, 441, 717
TasI AATT 2 cut(s) 310, 820
TatI WGTACW 2 cut(s) 225, 265
TfiI GAWTC 1 cut(s) 398
Tru1I TTAA 4 cut(s) 42, 93, 333, 823
Tru9I TTAA 4 cut(s) 42, 93, 333, 823
TscAI CASTG 2 cut(s) 25, 462
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 2 cut(s) 524, 794
Tsp45I GTSAC 1 cut(s) 16
TspRI CASTG 2 cut(s) 25, 462
Vha464I CTTAAG 1 cut(s) 332
XagI CCTNNNNNAGG 1 cut(s) 209
XceI RCATGY 1 cut(s) 771
XhoI CTCGAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.