Rmu_sc0000982.1_g000069

Cysteine desulfurase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000982.1
OG0018256
Physical Location & Seq
Reverse (-)
282626 .. 285495
2870 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 558 bp
atggttagaggggactgccgactaactacttcatgccatctggaggggactgccgaccagatgttgtaccggaggggactgccgacagaaatattctggaggggattgccgaccagaatattgtctggaggggactaccgaccagacttatctggaggggactgccgaccagtgaacttcaaggctcaaaatcgatctcctccgaccgaatcactgcaaaacactaccacagatgtaacaaggaggaagaggcgagcttgccgatgcccggattccgtcgtgggtcggccggagaaggaagaaatcgaaggaggaagaatgggaccgaagaggagaaaaggatcggggaagaagagagagaaaactagatcctactcacagtcggctaacatcatagtgcgattgttccctggttctgttcttcctattgaagatatcgcatgttgcgctcatgatgttaaggcaagagttcttgtagatgcttatcagagtgttccacacatggcggttgatgtacaggatcttaatgctgactttcttgttgcttcctctcactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00450 ko01100 map00450 map01100
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

185

Amino Acids

20.61

Weight (kDa)

9.44

Isoelectric Point (pI)

72.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 138 - 185 7.4e-12 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 506
AclWI GGATC 3 cut(s) 349, 363, 528
AcoI YGGCCR 1 cut(s) 287
AfaI GTAC 2 cut(s) 68, 516
AfiI CCNNNNNNNGG 2 cut(s) 43, 268
AgsI TTSAA 2 cut(s) 181, 431
AjnI CCWGG 1 cut(s) 409
AluBI AGCT 1 cut(s) 257
AluI AGCT 1 cut(s) 257
AlwI GGATC 3 cut(s) 349, 363, 528
AoxI GGCC 1 cut(s) 287
AspLEI GCGC 1 cut(s) 449
AspS9I GGNCC 1 cut(s) 323
AsuC2I CCSGG 1 cut(s) 269
AvaII GGWCC 1 cut(s) 323
BccI CCATC 1 cut(s) 45
BciT130I CCWGG 1 cut(s) 411
BcnI CCSGG 1 cut(s) 269
BfaI CTAG 2 cut(s) 366, 556
Bme1390I CCNGG 2 cut(s) 269, 411
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 2 cut(s) 269, 411
BmsI GCATC 2 cut(s) 254, 469
BpmI CTGGAG 4 cut(s) 62, 118, 147, 174
BpuMI CCSGG 1 cut(s) 269
Bsa29I ATCGAT 1 cut(s) 193
BsaBI GATNNNNATC 1 cut(s) 483
BsaJI CCNNGG 1 cut(s) 409
BsaWI WCCGGW 1 cut(s) 69
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 268
Bse1I ACTGG 1 cut(s) 170
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 1 cut(s) 411
BseCI ATCGAT 1 cut(s) 193
BseDI CCNNGG 1 cut(s) 409
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 2 cut(s) 43, 268
BseNI ACTGG 1 cut(s) 170
BseRI GAGGAG 2 cut(s) 189, 346
BseX3I CGGCCG 1 cut(s) 287
Bsh1285I CGRYCG 2 cut(s) 207, 290
BshFI GGCC 1 cut(s) 289
BshVI ATCGAT 1 cut(s) 193
BsiEI CGRYCG 2 cut(s) 207, 290
BsiSI CCGG 3 cut(s) 70, 269, 290
BslFI GGGAC 6 cut(s) 26, 61, 90, 146, 173, 336
BslI CCNNNNNNNGG 2 cut(s) 43, 268
BsmFI GGGAC 6 cut(s) 26, 61, 90, 146, 173, 336
BsnI GGCC 1 cut(s) 289
Bsp1407I TGTACA 1 cut(s) 514
Bsp143I GATC 4 cut(s) 194, 341, 368, 520
BspACI CCGC 1 cut(s) 506
BspANI GGCC 1 cut(s) 289
BspDI ATCGAT 1 cut(s) 193
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 1 cut(s) 324
BspPI GGATC 3 cut(s) 349, 363, 528
BsrGI TGTACA 1 cut(s) 514
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 409
BssMI GATC 4 cut(s) 194, 341, 368, 520
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 1 cut(s) 381
Bst6I CTCTTC 3 cut(s) 242, 323, 347
BstAUI TGTACA 1 cut(s) 514
BstC8I GCNNGC 2 cut(s) 255, 259
BstHHI GCGC 1 cut(s) 449
BstKTI GATC 4 cut(s) 197, 344, 371, 523
BstMBI GATC 4 cut(s) 194, 341, 368, 520
BstMCI CGRYCG 2 cut(s) 207, 290
BstMWI GCNNNNNNNGC 1 cut(s) 446
BstNI CCWGG 1 cut(s) 411
BstNSI RCATGY 1 cut(s) 444
BstSCI CCNGG 2 cut(s) 267, 409
BstX2I RGATCY 2 cut(s) 368, 520
BstYI RGATCY 2 cut(s) 368, 520
BstZI CGGCCG 1 cut(s) 287
Bsu15I ATCGAT 1 cut(s) 193
BsuRI GGCC 1 cut(s) 289
BsuTUI ATCGAT 1 cut(s) 193
BtsI GCAGTG 1 cut(s) 212
BtsIMutI CAGTG 2 cut(s) 177, 212
Cac8I GCNNGC 2 cut(s) 255, 259
CciI TCATGA 1 cut(s) 451
CfoI GCGC 1 cut(s) 449
Cfr13I GGNCC 1 cut(s) 323
ClaI ATCGAT 1 cut(s) 193
Csp6I GTAC 2 cut(s) 67, 515
CviAII CATG 4 cut(s) 33, 441, 452, 502
CviJI RGCY 4 cut(s) 185, 257, 289, 386
CviKI_1 RGCY 4 cut(s) 185, 257, 289, 386
CviQI GTAC 2 cut(s) 67, 515
DpnI GATC 4 cut(s) 196, 343, 370, 522
DpnII GATC 4 cut(s) 194, 341, 368, 520
EaeI YGGCCR 1 cut(s) 287
EagI CGGCCG 1 cut(s) 287
Eam1104I CTCTTC 3 cut(s) 242, 323, 347
EarI CTCTTC 3 cut(s) 242, 323, 347
EclXI CGGCCG 1 cut(s) 287
Eco32I GATATC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 323
Eco52I CGGCCG 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 409
EcoRV GATATC 1 cut(s) 436
FaeI CATG 4 cut(s) 36, 444, 455, 505
FaiI YATR 5 cut(s) 34, 395, 442, 453, 503
FaqI GGGAC 6 cut(s) 26, 61, 90, 146, 173, 336
FatI CATG 4 cut(s) 32, 440, 451, 501
FspBI CTAG 2 cut(s) 366, 556
GlaI GCGC 1 cut(s) 448
GsuI CTGGAG 4 cut(s) 62, 118, 147, 174
HaeIII GGCC 1 cut(s) 289
HapII CCGG 3 cut(s) 70, 269, 290
HhaI GCGC 1 cut(s) 449
Hin1II CATG 4 cut(s) 36, 444, 455, 505
Hin6I GCGC 1 cut(s) 447
HinP1I GCGC 1 cut(s) 447
HinfI GANTC 2 cut(s) 209, 272
HpaII CCGG 3 cut(s) 70, 269, 290
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 2 cut(s) 204, 489
Hpy188III TCNNGA 5 cut(s) 41, 97, 126, 153, 452
Hpy8I GTNNAC 1 cut(s) 175
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 2 cut(s) 289, 302
HpyCH4III ACNGT 1 cut(s) 381
HpyCH4V TGCA 1 cut(s) 217
HpyF10VI GCNNNNNNNGC 1 cut(s) 446
Hsp92II CATG 4 cut(s) 36, 444, 455, 505
HspAI GCGC 1 cut(s) 447
Kzo9I GATC 4 cut(s) 194, 341, 368, 520
LweI GCATC 2 cut(s) 254, 469
MaeI CTAG 2 cut(s) 366, 556
MaeIII GTNAC 1 cut(s) 235
MalI GATC 4 cut(s) 196, 343, 370, 522
MboI GATC 4 cut(s) 194, 341, 368, 520
MboII GAAGA 8 cut(s) 259, 311, 327, 340, 361, 364, 413, 443
MflI RGATCY 2 cut(s) 368, 520
MmeI TCCRAC 1 cut(s) 227
MseI TTAA 2 cut(s) 459, 525
MslI CAYNNNNRTG 1 cut(s) 395
MspI CCGG 3 cut(s) 70, 269, 290
MspR9I CCNGG 2 cut(s) 269, 411
MvaI CCWGG 1 cut(s) 411
MwoI GCNNNNNNNGC 1 cut(s) 446
NciI CCSGG 1 cut(s) 269
NdeII GATC 4 cut(s) 194, 341, 368, 520
NlaIII CATG 4 cut(s) 36, 444, 455, 505
NlaIV GGNNCC 1 cut(s) 324
NspI RCATGY 1 cut(s) 444
PagI TCATGA 1 cut(s) 451
PfeI GAWTC 2 cut(s) 209, 272
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 1 cut(s) 323
PsuI RGATCY 2 cut(s) 368, 520
RsaI GTAC 2 cut(s) 68, 516
RsaNI GTAC 2 cut(s) 67, 515
RseI CAYNNNNRTG 1 cut(s) 395
SaqAI TTAA 2 cut(s) 459, 525
Sau3AI GATC 4 cut(s) 194, 341, 368, 520
Sau96I GGNCC 1 cut(s) 323
ScrFI CCNGG 2 cut(s) 269, 411
SetI ASST 1 cut(s) 259
SfaNI GCATC 2 cut(s) 254, 469
SinI GGWCC 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 395
SsiI CCGC 1 cut(s) 506
SspI AATATT 2 cut(s) 93, 120
SspMI CTAG 2 cut(s) 366, 556
StyD4I CCNGG 2 cut(s) 267, 409
TaaI ACNGT 1 cut(s) 381
TaqI TCGA 2 cut(s) 193, 306
TaqII GACCGA 2 cut(s) 221, 340
TatI WGTACW 1 cut(s) 514
TfiI GAWTC 2 cut(s) 209, 272
Tru1I TTAA 2 cut(s) 459, 525
Tru9I TTAA 2 cut(s) 459, 525
TscAI CASTG 2 cut(s) 177, 219
TspDTI ATGAA 1 cut(s) 21
TspGWI ACGGA 1 cut(s) 265
TspRI CASTG 2 cut(s) 177, 219
VpaK11BI GGWCC 1 cut(s) 323
XceI RCATGY 1 cut(s) 444
XspI CTAG 2 cut(s) 366, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.