Rroxscaffold_4G00322620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018256
Physical Location & Seq
Forward (+)
53045244 .. 53059027
13784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00322620.1

Sequence Viewer

Length: 564 bp
ATGATCTATGTCAAAAACCGAACATTCGACGCCAATTTGGTAGCCGGAGACGGCCAGAATCCCGGACATCGACAAAACTCTCGTTCGGCTACGGTAACCTTCGTGGCTCAATTCCGAGCTCTCTGGGTCGAGCCACCGCAAAGCACCACCACGGGCGTGACCAGGGTGAAGAGGCGAGCCGGTGATGCCCGGATTCCGTCATGGGGTGGCCGGAGGAGGAAGAAATCGGAGGGAGAAGAAACCACCGAAGAAGAGAAAGGATCGGGGGAGAGGAGAGAGAAAAGGCTGGGAAAGAAGAGAGATGGGATCGGGGAGAGAGAGGACAGTTTACAATTGGGGACTGTTGTGAGAAAGAAAGGAAATTCTTTAGAGACACTACTTGGTACCGTTTGCGTTGGGTATTCAGTCTCCTTCGATGGAACTAATGACACATCTCTTTTACTACAATGCCTTTGCAATCAGCTTTACACCGAGCCAGCCCCGAGGGTAGCTACCTGTACCGAGGCAGTGCCTCGGGCCACCGGCTTCGGGGGCCTCCGAAGCTTTGAGCTCCAGTATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

20.6

Weight (kDa)

9.57

Isoelectric Point (pI)

57.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 383
AccB1I GGYRCC 1 cut(s) 383
AciI CCGC 1 cut(s) 137
AclWI GGATC 2 cut(s) 268, 314
AcoI YGGCCR 2 cut(s) 52, 208
AcsI RAATTY 1 cut(s) 361
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 2 cut(s) 385, 499
AfiI CCNNNNNNNGG 2 cut(s) 203, 528
AjnI CCWGG 1 cut(s) 161
AleI CACNNNNGTG 1 cut(s) 155
AluBI AGCT 5 cut(s) 119, 463, 491, 543, 550
AluI AGCT 5 cut(s) 119, 463, 491, 543, 550
Alw21I GWGCWC 2 cut(s) 121, 552
Alw26I GTCTC 3 cut(s) 42, 365, 412
AlwI GGATC 2 cut(s) 268, 314
Ama87I CYCGRG 2 cut(s) 481, 513
AoxI GGCC 4 cut(s) 52, 208, 516, 532
ApoI RAATTY 1 cut(s) 361
Asp718I GGTACC 1 cut(s) 383
AspS9I GGNCC 2 cut(s) 516, 532
AsuC2I CCSGG 2 cut(s) 63, 190
AsuHPI GGTGA 2 cut(s) 178, 194
AvaI CYCGRG 2 cut(s) 481, 513
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 2 cut(s) 121, 552
Bbv12I GWGCWC 2 cut(s) 121, 552
BccI CCATC 2 cut(s) 296, 410
BceAI ACGGC 1 cut(s) 67
BciT130I CCWGG 1 cut(s) 163
BcnI CCSGG 2 cut(s) 63, 190
BcoDI GTCTC 3 cut(s) 42, 365, 412
Bme1390I CCNGG 3 cut(s) 63, 163, 190
BmeT110I CYCGRG 2 cut(s) 481, 513
BmgT120I GGNCC 2 cut(s) 516, 532
BmiI GGNNCC 2 cut(s) 385, 533
BmrFI CCNGG 3 cut(s) 63, 163, 190
BmsI GCATC 1 cut(s) 175
BpmI CTGGAG 1 cut(s) 536
BpuMI CCSGG 2 cut(s) 63, 190
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 5 cut(s) 150, 162, 482, 501, 512
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 528
Bse118I RCCGGY 2 cut(s) 179, 521
Bse1I ACTGG 1 cut(s) 553
BseBI CCWGG 1 cut(s) 163
BseDI CCNNGG 5 cut(s) 150, 162, 482, 501, 512
BseLI CCNNNNNNNGG 2 cut(s) 203, 528
BseNI ACTGG 1 cut(s) 553
BseRI GAGGAG 2 cut(s) 229, 286
BseYI CCCAGC 1 cut(s) 286
BshFI GGCC 4 cut(s) 54, 210, 518, 534
BshNI GGYRCC 1 cut(s) 383
BsiHKAI GWGCWC 2 cut(s) 121, 552
BsiHKCI CYCGRG 2 cut(s) 481, 513
BsiSI CCGG 6 cut(s) 45, 63, 180, 190, 211, 522
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 2 cut(s) 203, 528
BsmAI GTCTC 3 cut(s) 42, 365, 412
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 1 cut(s) 352
BsnI GGCC 4 cut(s) 54, 210, 518, 534
BsoBI CYCGRG 2 cut(s) 481, 513
Bsp1286I GDGCHC 2 cut(s) 121, 552
Bsp143I GATC 3 cut(s) 3, 260, 306
BspACI CCGC 1 cut(s) 137
BspANI GGCC 4 cut(s) 54, 210, 518, 534
BspLI GGNNCC 2 cut(s) 385, 533
BspPI GGATC 2 cut(s) 268, 314
BspT107I GGYRCC 1 cut(s) 383
BsrFI RCCGGY 2 cut(s) 179, 521
BsrI ACTGG 1 cut(s) 553
BssAI RCCGGY 2 cut(s) 179, 521
BssECI CCNNGG 5 cut(s) 150, 162, 482, 501, 512
BssMI GATC 3 cut(s) 3, 260, 306
BssNI GRCGYC 1 cut(s) 30
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 4 cut(s) 94, 326, 343, 388
Bst6I CTCTTC 3 cut(s) 164, 246, 290
BstACI GRCGYC 1 cut(s) 30
BstC8I GCNNGC 2 cut(s) 177, 477
BstDEI CTNAG 1 cut(s) 561
BstDSI CCRYGG 1 cut(s) 150
BstEII GGTNACC 1 cut(s) 94
BstKTI GATC 3 cut(s) 6, 263, 309
BstMAI GTCTC 3 cut(s) 42, 365, 412
BstMBI GATC 3 cut(s) 3, 260, 306
BstMWI GCNNNNNNNGC 3 cut(s) 185, 531, 540
BstNI CCWGG 1 cut(s) 163
BstPI GGTNACC 1 cut(s) 94
BstSCI CCNGG 3 cut(s) 61, 161, 188
BsuRI GGCC 4 cut(s) 54, 210, 518, 534
BtgI CCRYGG 1 cut(s) 150
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 1 cut(s) 513
Cac8I GCNNGC 2 cut(s) 177, 477
Cfr10I RCCGGY 2 cut(s) 179, 521
Cfr13I GGNCC 2 cut(s) 516, 532
CseI GACGC 1 cut(s) 38
Csp6I GTAC 2 cut(s) 384, 498
CviAII CATG 1 cut(s) 201
CviQI GTAC 2 cut(s) 384, 498
DdeI CTNAG 1 cut(s) 561
DpnI GATC 3 cut(s) 5, 262, 308
DpnII GATC 3 cut(s) 3, 260, 306
EaeI YGGCCR 2 cut(s) 52, 208
Eam1104I CTCTTC 3 cut(s) 164, 246, 290
EarI CTCTTC 3 cut(s) 164, 246, 290
Ecl136II GAGCTC 2 cut(s) 119, 550
Eco24I GRGCYC 2 cut(s) 121, 552
Eco53kI GAGCTC 2 cut(s) 119, 550
Eco88I CYCGRG 2 cut(s) 481, 513
Eco91I GGTNACC 1 cut(s) 94
EcoICRI GAGCTC 2 cut(s) 119, 550
EcoO109I RGGNCCY 1 cut(s) 532
EcoO65I GGTNACC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 161
EcoT38I GRGCYC 2 cut(s) 121, 552
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 1 cut(s) 204
FaiI YATR 3 cut(s) 9, 202, 558
FaqI GGGAC 1 cut(s) 352
FatI CATG 1 cut(s) 200
FriOI GRGCYC 2 cut(s) 121, 552
GsaI CCCAGC 1 cut(s) 290
GsuI CTGGAG 1 cut(s) 536
HaeIII GGCC 4 cut(s) 54, 210, 518, 534
HapII CCGG 6 cut(s) 45, 63, 180, 190, 211, 522
HgaI GACGC 1 cut(s) 38
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 1 cut(s) 204
HindIII AAGCTT 1 cut(s) 541
HinfI GANTC 2 cut(s) 58, 193
HpaII CCGG 6 cut(s) 45, 63, 180, 190, 211, 522
HphI GGTGA 2 cut(s) 178, 194
Hpy166II GTNNAC 1 cut(s) 329
Hpy188I TCNGA 3 cut(s) 116, 229, 539
Hpy8I GTNNAC 1 cut(s) 329
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 109, 421
HpyCH4III ACNGT 4 cut(s) 94, 326, 343, 388
HpyCH4V TGCA 1 cut(s) 456
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 531, 540
HpyF3I CTNAG 1 cut(s) 561
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 1 cut(s) 204
KpnI GGTACC 1 cut(s) 387
Kzo9I GATC 3 cut(s) 3, 260, 306
LmnI GCTCC 1 cut(s) 555
LweI GCATC 1 cut(s) 175
MaeIII GTNAC 2 cut(s) 94, 157
MalI GATC 3 cut(s) 5, 262, 308
MboI GATC 3 cut(s) 3, 260, 306
MboII GAAGA 6 cut(s) 181, 232, 248, 260, 263, 307
MfeI CAATTG 1 cut(s) 332
MhlI GDGCHC 2 cut(s) 121, 552
MluCI AATT 4 cut(s) 34, 110, 332, 361
MslI CAYNNNNRTG 1 cut(s) 155
MspI CCGG 6 cut(s) 45, 63, 180, 190, 211, 522
MspR9I CCNGG 3 cut(s) 63, 163, 190
MunI CAATTG 1 cut(s) 332
MvaI CCWGG 1 cut(s) 163
MwoI GCNNNNNNNGC 3 cut(s) 185, 531, 540
NciI CCSGG 2 cut(s) 63, 190
NdeII GATC 3 cut(s) 3, 260, 306
NlaIII CATG 1 cut(s) 204
NlaIV GGNNCC 2 cut(s) 385, 533
NmuCI GTSAC 1 cut(s) 157
OliI CACNNNNGTG 1 cut(s) 155
PfeI GAWTC 2 cut(s) 58, 193
PfoI TCCNGGA 1 cut(s) 61
Psp124BI GAGCTC 2 cut(s) 121, 552
Psp6I CCWGG 1 cut(s) 161
PspEI GGTNACC 1 cut(s) 94
PspFI CCCAGC 1 cut(s) 286
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 2 cut(s) 385, 533
PspPI GGNCC 2 cut(s) 516, 532
RsaI GTAC 2 cut(s) 385, 499
RsaNI GTAC 2 cut(s) 384, 498
RseI CAYNNNNRTG 1 cut(s) 155
SacI GAGCTC 2 cut(s) 121, 552
Sau3AI GATC 3 cut(s) 3, 260, 306
Sau96I GGNCC 2 cut(s) 516, 532
ScrFI CCNGG 3 cut(s) 63, 163, 190
SduI GDGCHC 2 cut(s) 121, 552
SetI ASST 7 cut(s) 101, 121, 465, 493, 497, 545, 552
SfaNI GCATC 1 cut(s) 175
SmiMI CAYNNNNRTG 1 cut(s) 155
Sse9I AATT 4 cut(s) 34, 110, 332, 361
SsiI CCGC 1 cut(s) 137
SstI GAGCTC 2 cut(s) 121, 552
StyD4I CCNGG 3 cut(s) 61, 161, 188
TaaI ACNGT 4 cut(s) 94, 326, 343, 388
TaqI TCGA 4 cut(s) 27, 70, 129, 414
TasI AATT 4 cut(s) 34, 110, 332, 361
TfiI GAWTC 2 cut(s) 58, 193
TscAI CASTG 1 cut(s) 513
TseFI GTSAC 1 cut(s) 157
Tsp45I GTSAC 1 cut(s) 157
TspGWI ACGGA 1 cut(s) 186
TspRI CASTG 1 cut(s) 513
XapI RAATTY 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.