Rmu_sc0005278.1_g000007

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005278.1
OG0018256
Physical Location & Seq
Reverse (-)
44496 .. 47297
2802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005278.1_g000007.1.cds

Sequence Viewer

Length: 1152 bp
atggccggaatagccggaaatcgacaaaactcccaaactgctatagtgaacttcatggctaaattccgagctctaccggtcgagccaccgcaaaacaccaccacaggtgtgacaagaaggaggaggcgagcttaccggtgcccggattccatcgtggggtggccggagggggaagaaatcggaaggagaagaaattgggccgaagaggagaaagggtcaggggagaggagagagaaaagctgggtgggtcggatctggacgatgagtcgcggtgagtcgagaaatctcacacggtcaggggttagcgccgaagtctgggtcaaggatgtctctggtttggttggagttcttcgacgtcagcaattgcgctcgggtctcacggggctgggacaggtgttggagacggatcaccggcgacatggagtggttgggcacatcggagctgggcgcggaacaggaccgaaatcccgttcggttcgggtcaagcttgggtgtccagagaagaaatgcagacctttgggaacttcaatccaattccaaaccccaccaccaccactagaccaagaccaagaaggtccgatcaaaaccatatccagacataattccagttcccggaagaaaaaaaaagccgccggattaagaatcggttacgccagagatgccaacgctcactctctctctttcgacgccgatgacgtcatcattgccgacggaactgattcgcttcccgaacccagccattcttctatatcagtcttaaatagttctattgagacttcagtggaggatgacaagagagagaaagaatctctttgtaagaaaaaggaaactcttacggaagaactggagaaacttcttgctttgatctctgcttcgatctcctctgcttcgatcttcttgcttcgatcaaaaattgaacaaaccccaaaacagaggtcaacgcccaagaaggcagcgacctcgttctccacaacgactccggccccgacctcattctacacccacttcgtaccaacacccgtcggaccctttcacgcccagaagccaccggagccgctcctctacttcgatctcctctccgatctcaacttcgccgtcgccgaaaagcccaaggtccctctgcatatgctattaagagttgaagacctttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UVR

Protein Analysis

383

Amino Acids

42.28

Weight (kDa)

9.84

Isoelectric Point (pI)

59.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 358, 699
AccB1I GGYRCC 1 cut(s) 138
AccBSI CCGCTC 1 cut(s) 1057
AccII CGCG 2 cut(s) 270, 450
AciI CCGC 5 cut(s) 89, 270, 450, 630, 1055
AclWI GGATC 2 cut(s) 260, 414
AcoI YGGCCR 2 cut(s) 3, 161
AcsI RAATTY 1 cut(s) 62
AcuI CTGAAG 1 cut(s) 762
AcyI GRCGYC 3 cut(s) 355, 687, 696
AfaI GTAC 1 cut(s) 1011
AfiI CCNNNNNNNGG 4 cut(s) 142, 156, 315, 612
AgeI ACCGGT 2 cut(s) 76, 135
AgsI TTSAA 3 cut(s) 528, 917, 1142
AhdI GACNNNNNGTC 1 cut(s) 264
AleI CACNNNNGTG 1 cut(s) 107
AluBI AGCT 5 cut(s) 71, 131, 240, 443, 487
AluI AGCT 5 cut(s) 71, 131, 240, 443, 487
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 4 cut(s) 334, 380, 395, 767
AlwI GGATC 2 cut(s) 260, 414
Ama87I CYCGRG 1 cut(s) 370
AoxI GGCC 4 cut(s) 3, 161, 198, 981
ApeKI GCWGC 1 cut(s) 953
ApoI RAATTY 1 cut(s) 62
ArsI GACNNNNNNTTYG 4 cut(s) 303, 335, 920, 952
AsiGI ACCGGT 2 cut(s) 76, 135
Asp700I GAANNNNTTC 1 cut(s) 718
AspLEI GCGC 3 cut(s) 308, 369, 450
AspS9I GGNCC 6 cut(s) 198, 458, 575, 982, 1025, 1114
AsuC2I CCSGG 2 cut(s) 143, 613
AsuHPI GGTGA 2 cut(s) 284, 401
AvaI CYCGRG 1 cut(s) 370
AvaII GGWCC 4 cut(s) 458, 575, 1025, 1114
BaeGI GKGCMC 2 cut(s) 143, 435
BanI GGYRCC 1 cut(s) 138
BanII GRGCYC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 1 cut(s) 965
BccI CCATC 1 cut(s) 158
BceAI ACGGC 1 cut(s) 1079
BcgI CGANNNNNNTGC 4 cut(s) 880, 914, 942, 976
BcnI CCSGG 2 cut(s) 143, 613
BcoDI GTCTC 4 cut(s) 334, 380, 395, 767
BfaI CTAG 1 cut(s) 557
BfmI CTRYAG 1 cut(s) 42
BfoI RGCGCY 1 cut(s) 309
BisI GCNGC 3 cut(s) 630, 954, 1055
BlsI GCNGC 3 cut(s) 631, 955, 1056
Bme1390I CCNGG 2 cut(s) 143, 613
Bme18I GGWCC 4 cut(s) 458, 575, 1025, 1114
BmeRI GACNNNNNGTC 1 cut(s) 264
BmeT110I CYCGRG 1 cut(s) 370
BmgT120I GGNCC 6 cut(s) 198, 458, 575, 982, 1025, 1114
BmiI GGNNCC 5 cut(s) 140, 984, 1027, 1053, 1116
BmrFI CCNGG 2 cut(s) 143, 613
BmsI GCATC 1 cut(s) 649
BpmI CTGGAG 1 cut(s) 866
BpuMI CCSGG 2 cut(s) 143, 613
BsaHI GRCGYC 3 cut(s) 355, 687, 696
BsaI GGTCTC 1 cut(s) 380
BsaJI CCNNGG 1 cut(s) 1110
BsaWI WCCGGW 3 cut(s) 76, 135, 1048
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 961, 991
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 156, 315, 612
Bse118I RCCGGY 3 cut(s) 76, 135, 411
Bse1I ACTGG 2 cut(s) 606, 849
Bse3DI GCAATG 1 cut(s) 702
BseDI CCNNGG 1 cut(s) 1110
BseGI GGATG 2 cut(s) 331, 793
BseLI CCNNNNNNNGG 4 cut(s) 142, 156, 315, 612
BseMI GCAATG 1 cut(s) 702
BseNI ACTGG 2 cut(s) 606, 849
BseRI GAGGAG 6 cut(s) 136, 221, 241, 871, 1049, 1064
BseSI GKGCMC 2 cut(s) 143, 435
BseXI GCAGC 1 cut(s) 965
BseYI CCCAGC 4 cut(s) 240, 385, 443, 734
Bsh1236I CGCG 2 cut(s) 270, 450
Bsh1285I CGRYCG 1 cut(s) 81
BshFI GGCC 4 cut(s) 5, 163, 200, 983
BshNI GGYRCC 1 cut(s) 138
BshTI ACCGGT 2 cut(s) 76, 135
BsiEI CGRYCG 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 73
BsiHKCI CYCGRG 1 cut(s) 370
BslFI GGGAC 2 cut(s) 402, 1100
BslI CCNNNNNNNGG 4 cut(s) 142, 156, 315, 612
BsmAI GTCTC 4 cut(s) 334, 380, 395, 767
BsmBI CGTCTC 1 cut(s) 395
BsmFI GGGAC 2 cut(s) 402, 1100
BsnI GGCC 4 cut(s) 5, 163, 200, 983
Bso31I GGTCTC 1 cut(s) 380
BsoBI CYCGRG 1 cut(s) 370
Bsp1286I GDGCHC 3 cut(s) 73, 143, 435
Bsp143I GATC 9 cut(s) 252, 406, 579, 864, 876, 891, 905, 1069, 1081
BspACI CCGC 5 cut(s) 89, 270, 450, 630, 1055
BspANI GGCC 4 cut(s) 5, 163, 200, 983
BspFNI CGCG 2 cut(s) 270, 450
BspLI GGNNCC 5 cut(s) 140, 984, 1027, 1053, 1116
BspPI GGATC 2 cut(s) 260, 414
BspT107I GGYRCC 1 cut(s) 138
BspTNI GGTCTC 1 cut(s) 380
BsrBI CCGCTC 1 cut(s) 1057
BsrDI GCAATG 1 cut(s) 702
BsrFI RCCGGY 3 cut(s) 76, 135, 411
BsrI ACTGG 2 cut(s) 606, 849
BssAI RCCGGY 3 cut(s) 76, 135, 411
BssECI CCNNGG 1 cut(s) 1110
BssMI GATC 9 cut(s) 252, 406, 579, 864, 876, 891, 905, 1069, 1081
BssNI GRCGYC 3 cut(s) 355, 687, 696
BssT1I CCWWGG 1 cut(s) 1110
Bst4CI ACNGT 1 cut(s) 294
Bst6I CTCTTC 1 cut(s) 198
BstACI GRCGYC 3 cut(s) 355, 687, 696
BstC8I GCNNGC 1 cut(s) 129
BstF5I GGATG 2 cut(s) 331, 793
BstFNI CGCG 2 cut(s) 270, 450
BstH2I RGCGCY 1 cut(s) 309
BstHHI GCGC 3 cut(s) 308, 369, 450
BstKTI GATC 9 cut(s) 255, 409, 582, 867, 879, 894, 908, 1072, 1084
BstMAI GTCTC 4 cut(s) 334, 380, 395, 767
BstMBI GATC 9 cut(s) 252, 406, 579, 864, 876, 891, 905, 1069, 1081
BstMCI CGRYCG 1 cut(s) 81
BstMWI GCNNNNNNNGC 3 cut(s) 11, 659, 1051
BstSCI CCNGG 2 cut(s) 141, 611
BstSFI CTRYAG 1 cut(s) 42
BstSLI GKGCMC 2 cut(s) 143, 435
BstUI CGCG 2 cut(s) 270, 450
BstV1I GCAGC 1 cut(s) 965
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BsuRI GGCC 4 cut(s) 5, 163, 200, 983
BtsCI GGATG 2 cut(s) 331, 793
BtsIMutI CAGTG 1 cut(s) 786
Cac8I GCNNGC 1 cut(s) 129
CfoI GCGC 3 cut(s) 308, 369, 450
Cfr10I RCCGGY 3 cut(s) 76, 135, 411
Cfr13I GGNCC 6 cut(s) 198, 458, 575, 982, 1025, 1114
CseI GACGC 1 cut(s) 695
Csp6I GTAC 1 cut(s) 1010
CspAI ACCGGT 2 cut(s) 76, 135
CviAII CATG 2 cut(s) 55, 419
CviQI GTAC 1 cut(s) 1010
DpnI GATC 9 cut(s) 254, 408, 581, 866, 878, 893, 907, 1071, 1083
DpnII GATC 9 cut(s) 252, 406, 579, 864, 876, 891, 905, 1069, 1081
DriI GACNNNNNGTC 1 cut(s) 264
EaeI YGGCCR 2 cut(s) 3, 161
Eam1104I CTCTTC 1 cut(s) 198
Eam1105I GACNNNNNGTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 198
Ecl136II GAGCTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 1110
Eco24I GRGCYC 1 cut(s) 73
Eco31I GGTCTC 1 cut(s) 380
Eco47I GGWCC 4 cut(s) 458, 575, 1025, 1114
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 762
Eco88I CYCGRG 1 cut(s) 370
EcoICRI GAGCTC 1 cut(s) 71
EcoO109I RGGNCCY 1 cut(s) 1114
EcoT14I CCWWGG 1 cut(s) 1110
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 1110
Esp3I CGTCTC 1 cut(s) 395
FaeI CATG 2 cut(s) 58, 422
FaiI YATR 8 cut(s) 44, 56, 420, 590, 600, 749, 1125, 1127
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FaqI GGGAC 2 cut(s) 402, 1100
FatI CATG 2 cut(s) 54, 418
FauNDI CATATG 1 cut(s) 1125
Fnu4HI GCNGC 3 cut(s) 630, 954, 1055
FokI GGATG 2 cut(s) 338, 800
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 3 cut(s) 630, 954, 1055
FspBI CTAG 1 cut(s) 557
GlaI GCGC 3 cut(s) 307, 368, 449
GluI GCNGC 3 cut(s) 630, 954, 1055
GsaI CCCAGC 4 cut(s) 244, 389, 447, 738
GsuI CTGGAG 1 cut(s) 866
HaeII RGCGCY 1 cut(s) 309
HaeIII GGCC 4 cut(s) 5, 163, 200, 983
HgaI GACGC 1 cut(s) 695
HhaI GCGC 3 cut(s) 308, 369, 450
Hin1I GRCGYC 3 cut(s) 355, 687, 696
Hin1II CATG 2 cut(s) 58, 422
Hin6I GCGC 3 cut(s) 306, 367, 448
HinP1I GCGC 3 cut(s) 306, 367, 448
HincII GTYRAC 1 cut(s) 939
HindII GTYRAC 1 cut(s) 939
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 7 cut(s) 146, 265, 275, 642, 719, 806, 976
HphI GGTGA 2 cut(s) 284, 401
Hpy166II GTNNAC 2 cut(s) 49, 939
Hpy188I TCNGA 7 cut(s) 68, 182, 252, 440, 579, 1025, 1081
Hpy188III TCNNGA 5 cut(s) 256, 279, 497, 594, 728
Hpy8I GTNNAC 2 cut(s) 49, 939
Hpy99I CGWCG 5 cut(s) 357, 689, 713, 1025, 1100
HpyAV CCTTC 4 cut(s) 111, 177, 566, 943
HpyCH4III ACNGT 1 cut(s) 294
HpyCH4IV ACGT 2 cut(s) 355, 696
HpyCH4V TGCA 2 cut(s) 510, 1123
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 659, 1051
HpySE526I ACGT 2 cut(s) 355, 696
Hsp92I GRCGYC 3 cut(s) 355, 687, 696
Hsp92II CATG 2 cut(s) 58, 422
HspAI GCGC 3 cut(s) 306, 367, 448
Kzo9I GATC 9 cut(s) 252, 406, 579, 864, 876, 891, 905, 1069, 1081
LmnI GCTCC 3 cut(s) 440, 1051, 1062
Lsp1109I GCAGC 1 cut(s) 965
LweI GCATC 1 cut(s) 649
MaeI CTAG 1 cut(s) 557
MaeII ACGT 2 cut(s) 355, 696
MaeIII GTNAC 2 cut(s) 109, 647
MalI GATC 9 cut(s) 254, 408, 581, 866, 878, 893, 907, 1071, 1083
MbiI CCGCTC 1 cut(s) 1057
MboI GATC 9 cut(s) 252, 406, 579, 864, 876, 891, 905, 1069, 1081
MboII GAAGA 9 cut(s) 185, 201, 215, 341, 514, 628, 735, 851, 886
MfeI CAATTG 1 cut(s) 362
MflI RGATCY 1 cut(s) 252
MhlI GDGCHC 3 cut(s) 73, 143, 435
MluCI AATT 6 cut(s) 62, 193, 362, 533, 601, 912
MlyI GAGTC 3 cut(s) 274, 284, 970
MmeI TCCRAC 4 cut(s) 230, 322, 378, 1003
MroXI GAANNNNTTC 1 cut(s) 718
MseI TTAA 3 cut(s) 638, 758, 1133
MslI CAYNNNNRTG 1 cut(s) 107
MspR9I CCNGG 2 cut(s) 143, 613
MunI CAATTG 1 cut(s) 362
MvnI CGCG 2 cut(s) 270, 450
MwoI GCNNNNNNNGC 3 cut(s) 11, 659, 1051
NciI CCSGG 2 cut(s) 143, 613
NdeI CATATG 1 cut(s) 1125
NdeII GATC 9 cut(s) 252, 406, 579, 864, 876, 891, 905, 1069, 1081
NlaIII CATG 2 cut(s) 58, 422
NlaIV GGNNCC 5 cut(s) 140, 984, 1027, 1053, 1116
NmuCI GTSAC 1 cut(s) 109
OliI CACNNNNGTG 1 cut(s) 107
PcsI WCGNNNNNNNCGW 2 cut(s) 693, 1098
PdmI GAANNNNTTC 1 cut(s) 718
PfeI GAWTC 4 cut(s) 146, 642, 719, 806
PfoI TCCNGGA 1 cut(s) 611
PinAI ACCGGT 2 cut(s) 76, 135
PkrI GCNGC 3 cut(s) 631, 955, 1056
PleI GAGTC 3 cut(s) 273, 283, 970
PpsI GAGTC 3 cut(s) 273, 283, 970
PpuMI RGGWCCY 1 cut(s) 1114
Psp124BI GAGCTC 1 cut(s) 73
Psp5II RGGWCCY 1 cut(s) 1114
PspFI CCCAGC 4 cut(s) 240, 385, 443, 734
PspN4I GGNNCC 5 cut(s) 140, 984, 1027, 1053, 1116
PspPI GGNCC 6 cut(s) 198, 458, 575, 982, 1025, 1114
PspPPI RGGWCCY 1 cut(s) 1114
PsuI RGATCY 1 cut(s) 252
RsaI GTAC 1 cut(s) 1011
RsaNI GTAC 1 cut(s) 1010
RseI CAYNNNNRTG 1 cut(s) 107
SacI GAGCTC 1 cut(s) 73
SaqAI TTAA 3 cut(s) 638, 758, 1133
SatI GCNGC 3 cut(s) 630, 954, 1055
Sau3AI GATC 9 cut(s) 252, 406, 579, 864, 876, 891, 905, 1069, 1081
Sau96I GGNCC 6 cut(s) 198, 458, 575, 982, 1025, 1114
SchI GAGTC 3 cut(s) 274, 284, 970
ScrFI CCNGG 2 cut(s) 143, 613
SduI GDGCHC 3 cut(s) 73, 143, 435
SfaNI GCATC 1 cut(s) 649
SfcI CTRYAG 1 cut(s) 42
SgrAI CRCCGGYG 1 cut(s) 411
SinI GGWCC 4 cut(s) 458, 575, 1025, 1114
SmiMI CAYNNNNRTG 1 cut(s) 107
Sse9I AATT 6 cut(s) 62, 193, 362, 533, 601, 912
SsiI CCGC 5 cut(s) 89, 270, 450, 630, 1055
SspMI CTAG 1 cut(s) 557
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 2 cut(s) 141, 611
StyI CCWWGG 1 cut(s) 1110
TaaI ACNGT 1 cut(s) 294
TaiI ACGT 2 cut(s) 358, 699
TaqI TCGA 9 cut(s) 22, 81, 278, 352, 684, 875, 890, 904, 1068
TaqII GACCGA 1 cut(s) 475
TasI AATT 6 cut(s) 62, 193, 362, 533, 601, 912
TauI GCSGC 2 cut(s) 632, 1057
TfiI GAWTC 4 cut(s) 146, 642, 719, 806
Tru1I TTAA 3 cut(s) 638, 758, 1133
Tru9I TTAA 3 cut(s) 638, 758, 1133
TscAI CASTG 1 cut(s) 786
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 1 cut(s) 953
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 43
TspGWI ACGGA 3 cut(s) 419, 726, 851
TspRI CASTG 1 cut(s) 786
VpaK11BI GGWCC 4 cut(s) 458, 575, 1025, 1114
XapI RAATTY 1 cut(s) 62
XmnI GAANNNNTTC 1 cut(s) 718
XspI CTAG 1 cut(s) 557
ZraI GACGTC 2 cut(s) 356, 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.