Rh4DG032300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0018256
Physical Location & Seq
Forward (+)
5438987 .. 5440935
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG032300.1

Sequence Viewer

Length: 267 bp
ATGGCAAATGCCGTCTTTCTGCCTTGCCATGCTTGCACTTTGTTGAACCCCGAATCCATCTTCAAGCACAGCGACTTCAACTCCATCAACAACCCCTCGCTATTATCCACCTCGCCGTACTCATCATCGGAGTGGGCTTCTCTTCAGGAGCTTCAACGCGGCCTTCTTGGTCTCCTCGACCTTGACGAGCGCGATCTCGACGCTTTGGTTGATGGTGATCTCTCGCTGCTCGAGCTCGTCAAGGCTCTCCTTGATCTCGCGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

88

Amino Acids

9.61

Weight (kDa)

4.16

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 159, 192, 260
AciI CCGC 1 cut(s) 159
AcuI CTGAAG 1 cut(s) 128
AfaI GTAC 1 cut(s) 119
AgsI TTSAA 4 cut(s) 46, 64, 79, 155
AloI GAACNNNNNNTCC 2 cut(s) 38, 70
AluBI AGCT 2 cut(s) 151, 235
AluI AGCT 2 cut(s) 151, 235
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 176
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 1 cut(s) 160
ApeKI GCWGC 1 cut(s) 226
AspLEI GCGC 2 cut(s) 192, 262
AsuHPI GGTGA 1 cut(s) 227
AvaI CYCGRG 1 cut(s) 230
BanII GRGCYC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 1 cut(s) 213
BccI CCATC 3 cut(s) 65, 92, 206
BceAI ACGGC 1 cut(s) 100
BcoDI GTCTC 1 cut(s) 176
BfaI CTAG 1 cut(s) 265
BisI GCNGC 2 cut(s) 160, 227
BlsI GCNGC 2 cut(s) 161, 228
BmeT110I CYCGRG 1 cut(s) 230
BsaBI GATNNNNATC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 176
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bse8I GATNNNNATC 1 cut(s) 216
BseJI GATNNNNATC 1 cut(s) 216
BseRI GAGGAG 1 cut(s) 164
BseXI GCAGC 1 cut(s) 213
Bsh1236I CGCG 3 cut(s) 159, 192, 260
BshFI GGCC 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 230
BsmAI GTCTC 1 cut(s) 176
BsnI GGCC 1 cut(s) 162
Bso31I GGTCTC 1 cut(s) 176
BsoBI CYCGRG 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 3 cut(s) 193, 217, 253
BspACI CCGC 1 cut(s) 159
BspANI GGCC 1 cut(s) 162
BspFNI CGCG 3 cut(s) 159, 192, 260
BspTNI GGTCTC 1 cut(s) 176
BssMI GATC 3 cut(s) 193, 217, 253
Bst6I CTCTTC 1 cut(s) 147
BstC8I GCNNGC 1 cut(s) 34
BstFNI CGCG 3 cut(s) 159, 192, 260
BstHHI GCGC 2 cut(s) 192, 262
BstKTI GATC 3 cut(s) 196, 220, 256
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 3 cut(s) 193, 217, 253
BstMWI GCNNNNNNNGC 2 cut(s) 33, 232
BstUI CGCG 3 cut(s) 159, 192, 260
BstV1I GCAGC 1 cut(s) 213
BsuRI GGCC 1 cut(s) 162
Cac8I GCNNGC 1 cut(s) 34
CfoI GCGC 2 cut(s) 192, 262
CseI GACGC 1 cut(s) 209
Csp6I GTAC 1 cut(s) 118
CviAII CATG 1 cut(s) 29
CviJI RGCY 5 cut(s) 137, 151, 162, 235, 245
CviKI_1 RGCY 5 cut(s) 137, 151, 162, 235, 245
CviQI GTAC 1 cut(s) 118
DpnI GATC 3 cut(s) 195, 219, 255
DpnII GATC 3 cut(s) 193, 217, 253
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Ecl136II GAGCTC 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 1 cut(s) 176
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 128
Eco88I CYCGRG 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 237
FaeI CATG 1 cut(s) 32
FaiI YATR 1 cut(s) 30
FatI CATG 1 cut(s) 28
Fnu4HI GCNGC 2 cut(s) 160, 227
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 2 cut(s) 160, 227
FspBI CTAG 1 cut(s) 265
GlaI GCGC 2 cut(s) 191, 261
GluI GCNGC 2 cut(s) 160, 227
HaeIII GGCC 1 cut(s) 162
HgaI GACGC 1 cut(s) 209
HhaI GCGC 2 cut(s) 192, 262
Hin1II CATG 1 cut(s) 32
Hin6I GCGC 2 cut(s) 190, 260
HinP1I GCGC 2 cut(s) 190, 260
HinfI GANTC 1 cut(s) 53
HphI GGTGA 1 cut(s) 227
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 2 cut(s) 146, 197
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 1 cut(s) 173
HpyCH4V TGCA 1 cut(s) 36
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 232
Hsp92II CATG 1 cut(s) 32
HspAI GCGC 2 cut(s) 190, 260
Kzo9I GATC 3 cut(s) 193, 217, 253
LmnI GCTCC 1 cut(s) 148
LpnPI CCDG 1 cut(s) 131
Lsp1109I GCAGC 1 cut(s) 213
MaeI CTAG 1 cut(s) 265
MalI GATC 3 cut(s) 195, 219, 255
MboI GATC 3 cut(s) 193, 217, 253
MboII GAAGA 2 cut(s) 52, 134
MhlI GDGCHC 1 cut(s) 237
MnlI CCTC 3 cut(s) 106, 121, 185
MslI CAYNNNNRTG 1 cut(s) 130
MvnI CGCG 3 cut(s) 159, 192, 260
MwoI GCNNNNNNNGC 2 cut(s) 33, 232
NdeII GATC 3 cut(s) 193, 217, 253
NlaIII CATG 1 cut(s) 32
PaeR7I CTCGAG 1 cut(s) 230
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PfeI GAWTC 1 cut(s) 53
PkrI GCNGC 2 cut(s) 161, 228
Psp124BI GAGCTC 1 cut(s) 237
PspXI VCTCGAGB 1 cut(s) 230
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
RseI CAYNNNNRTG 1 cut(s) 130
SacI GAGCTC 1 cut(s) 237
SatI GCNGC 2 cut(s) 160, 227
Sau3AI GATC 3 cut(s) 193, 217, 253
SduI GDGCHC 1 cut(s) 237
SetI ASST 4 cut(s) 113, 153, 183, 237
Sfr274I CTCGAG 1 cut(s) 230
SlaI CTCGAG 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 130
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
SsiI CCGC 1 cut(s) 159
SspMI CTAG 1 cut(s) 265
SstI GAGCTC 1 cut(s) 237
TaqI TCGA 3 cut(s) 177, 198, 231
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 1 cut(s) 53
TseI GCWGC 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 230
XspI CTAG 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.