Rmu_sc0000998.1_g000004

Protein of unknown function (DUF3339)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000998.1
OG0018714
Physical Location & Seq
Forward (+)
10921 .. 13830
2910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000998.1_g000004.1.cds

Sequence Viewer

Length: 669 bp
atggaagcagcgggcagtggaatcggatggctgaagcagctagccgccgccgccggtgtagatgtgaacaccaatagctatgagaaagacggtgatgagtgcaaagtaaatgatggtgtgcaccaaaatggaaactccactcgtctgcatgttgttaaactccaccactcggctgttcccggagcagagggaatgatgagtgctgattgggggccggtcgttgttgcggtggtcttgttcattctcttgtcgccggggctgctattccaattaccggcaaggacgagggtgatggagttcggaaacatgatgacaagcgggattgctatcctggttcatgccatcatatacttctgcatacttactatcttgatcattgcaattgaaaaggctaaagagatacagagacacacagatccaaagctgggggagagtaccggatcatcaaccggtgtagatgtggacgtgtacggccaagatgagaactgcatagagcgcaaaaaaaatgagtgtgtgcacagctatggctttgccgttggtcttcatgctcccaaactcgacctgcttgccgtttcccggcaacgagaagaggagtcccggttggagcagaataaacagcacaaccccaatcagtactgggccccagtccgccatttccttcaaagttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3339

Protein Analysis

222

Amino Acids

24.34

Weight (kDa)

6.04

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018714)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27027 AT5G40980
malus_domestica MD09G1009300.v1.1 MD17G1001100.v1.1
prunus_persica Prupe.3G307600_v2.0.a1
pyrus_communis pycom111g00750
rosa_multiflora Rmu_sc0000998.1_g000004
rosa_samantha Rh2DG692000
rosa_wichuraiana Rw2G054680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 570
AciI CCGC 7 cut(s) 11, 45, 48, 51, 227, 318, 649
AclWI GGATC 2 cut(s) 410, 448
AcoI YGGCCR 1 cut(s) 472
AcuI CTGAAG 1 cut(s) 53
AfaI GTAC 3 cut(s) 436, 470, 635
AfiI CCNNNNNNNGG 4 cut(s) 169, 274, 425, 576
AflIII ACRYGT 1 cut(s) 465
AgeI ACCGGT 1 cut(s) 449
AgsI TTSAA 2 cut(s) 386, 662
AjiI CACGTC 1 cut(s) 466
AjnI CCWGG 1 cut(s) 330
AluBI AGCT 4 cut(s) 40, 78, 424, 522
AluI AGCT 4 cut(s) 40, 78, 424, 522
Alw21I GWGCWC 2 cut(s) 123, 519
Alw26I GTCTC 1 cut(s) 400
Alw44I GTGCAC 2 cut(s) 119, 515
AlwI GGATC 2 cut(s) 410, 448
AoxI GGCC 3 cut(s) 212, 472, 639
ApaI GGGCCC 1 cut(s) 643
ApaLI GTGCAC 2 cut(s) 119, 515
ApeKI GCWGC 3 cut(s) 8, 37, 259
AsiGI ACCGGT 1 cut(s) 449
AspLEI GCGC 1 cut(s) 498
AspS9I GGNCC 3 cut(s) 212, 639, 640
AsuC2I CCSGG 4 cut(s) 180, 255, 577, 598
AsuHPI GGTGA 2 cut(s) 104, 301
AsuNHI GCTAGC 1 cut(s) 40
BaeGI GKGCMC 3 cut(s) 123, 519, 643
BanII GRGCYC 1 cut(s) 643
BbsI GAAGAC 1 cut(s) 533
Bbv12I GWGCWC 2 cut(s) 123, 519
BbvI GCAGC 3 cut(s) 20, 49, 246
BccI CCATC 4 cut(s) 21, 107, 286, 350
BceAI ACGGC 3 cut(s) 487, 518, 554
BcgI CGANNNNNNTGC 4 cut(s) 4, 38, 548, 582
BciT130I CCWGG 1 cut(s) 332
BclI TGATCA 1 cut(s) 372
BcnI CCSGG 4 cut(s) 180, 255, 577, 598
BcoDI GTCTC 1 cut(s) 400
BfaI CTAG 1 cut(s) 41
BfuAI ACCTGC 1 cut(s) 570
BisI GCNGC 6 cut(s) 9, 38, 45, 48, 51, 260
BlsI GCNGC 6 cut(s) 10, 39, 46, 49, 52, 261
BmcAI AGTACT 1 cut(s) 635
Bme1390I CCNGG 5 cut(s) 180, 255, 332, 577, 598
BmgBI CACGTC 1 cut(s) 466
BmgT120I GGNCC 3 cut(s) 212, 639, 640
BmiI GGNNCC 3 cut(s) 213, 641, 642
BmrFI CCNGG 5 cut(s) 180, 255, 332, 577, 598
BmrI ACTGGG 2 cut(s) 638, 646
BmtI GCTAGC 1 cut(s) 44
BmuI ACTGGG 2 cut(s) 638, 646
BpiI GAAGAC 1 cut(s) 533
BpuMI CCSGG 4 cut(s) 180, 255, 577, 598
BsaBI GATNNNNATC 1 cut(s) 326
BsaJI CCNNGG 1 cut(s) 254
BsaWI WCCGGW 2 cut(s) 437, 449
BsaXI ACNNNNNCTCC 2 cut(s) 584, 614
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 274, 425, 576
Bse118I RCCGGY 4 cut(s) 53, 214, 274, 449
Bse1I ACTGG 2 cut(s) 641, 644
Bse3DI GCAATG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 326
BseBI CCWGG 1 cut(s) 332
BseDI CCNNGG 1 cut(s) 254
BseGI GGATG 1 cut(s) 32
BseJI GATNNNNATC 1 cut(s) 326
BseLI CCNNNNNNNGG 4 cut(s) 169, 274, 425, 576
BseMI GCAATG 1 cut(s) 375
BseNI ACTGG 2 cut(s) 641, 644
BseRI GAGGAG 1 cut(s) 605
BseSI GKGCMC 3 cut(s) 123, 519, 643
BseXI GCAGC 3 cut(s) 20, 49, 246
BseYI CCCAGC 1 cut(s) 424
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 3 cut(s) 214, 474, 641
BshTI ACCGGT 1 cut(s) 449
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 2 cut(s) 123, 519
BsiSI CCGG 9 cut(s) 54, 180, 215, 254, 275, 438, 450, 577, 598
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 4 cut(s) 169, 274, 425, 576
BsmAI GTCTC 1 cut(s) 400
BsmFI GGGAC 1 cut(s) 580
BsnI GGCC 3 cut(s) 214, 474, 641
Bsp120I GGGCCC 1 cut(s) 639
Bsp1286I GDGCHC 3 cut(s) 123, 519, 643
Bsp143I GATC 3 cut(s) 372, 415, 440
BspACI CCGC 7 cut(s) 11, 45, 48, 51, 227, 318, 649
BspANI GGCC 3 cut(s) 214, 474, 641
BspLI GGNNCC 3 cut(s) 213, 641, 642
BspMI ACCTGC 1 cut(s) 570
BspOI GCTAGC 1 cut(s) 44
BspPI GGATC 2 cut(s) 410, 448
BsrDI GCAATG 1 cut(s) 375
BsrFI RCCGGY 4 cut(s) 53, 214, 274, 449
BsrI ACTGG 2 cut(s) 641, 644
BssAI RCCGGY 4 cut(s) 53, 214, 274, 449
BssECI CCNNGG 1 cut(s) 254
BssMI GATC 3 cut(s) 372, 415, 440
Bst2UI CCWGG 1 cut(s) 332
Bst4CI ACNGT 1 cut(s) 92
Bst6I CTCTTC 1 cut(s) 582
BstC8I GCNNGC 3 cut(s) 13, 42, 567
BstF5I GGATG 1 cut(s) 32
BstHHI GCGC 1 cut(s) 498
BstKTI GATC 3 cut(s) 375, 418, 443
BstMAI GTCTC 1 cut(s) 400
BstMBI GATC 3 cut(s) 372, 415, 440
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 4 cut(s) 37, 50, 259, 495
BstNI CCWGG 1 cut(s) 332
BstNSI RCATGY 1 cut(s) 152
BstSCI CCNGG 5 cut(s) 178, 253, 330, 575, 596
BstSLI GKGCMC 3 cut(s) 123, 519, 643
BstV1I GCAGC 3 cut(s) 20, 49, 246
BstV2I GAAGAC 1 cut(s) 533
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 3 cut(s) 214, 474, 641
BtrI CACGTC 1 cut(s) 466
BtsCI GGATG 1 cut(s) 32
BtsI GCAGTG 1 cut(s) 22
BtsIMutI CAGTG 1 cut(s) 22
BveI ACCTGC 1 cut(s) 570
Cac8I GCNNGC 3 cut(s) 13, 42, 567
CfoI GCGC 1 cut(s) 498
Cfr10I RCCGGY 4 cut(s) 53, 214, 274, 449
Cfr13I GGNCC 3 cut(s) 212, 639, 640
Csp6I GTAC 3 cut(s) 435, 469, 634
CspAI ACCGGT 1 cut(s) 449
CviAII CATG 4 cut(s) 149, 307, 338, 545
CviQI GTAC 3 cut(s) 435, 469, 634
DpnI GATC 3 cut(s) 374, 417, 442
DpnII GATC 3 cut(s) 372, 415, 440
EaeI YGGCCR 1 cut(s) 472
Eam1104I CTCTTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 582
EciI GGCGGA 1 cut(s) 638
Eco24I GRGCYC 1 cut(s) 643
Eco57I CTGAAG 1 cut(s) 53
EcoO109I RGGNCCY 1 cut(s) 640
EcoRII CCWGG 1 cut(s) 330
EcoT38I GRGCYC 1 cut(s) 643
FaeI CATG 4 cut(s) 152, 310, 341, 548
FaqI GGGAC 1 cut(s) 580
FatI CATG 4 cut(s) 148, 306, 337, 544
FauI CCCGC 2 cut(s) 4, 311
FbaI TGATCA 1 cut(s) 372
Fnu4HI GCNGC 6 cut(s) 9, 38, 45, 48, 51, 260
FokI GGATG 1 cut(s) 39
FriOI GRGCYC 1 cut(s) 643
Fsp4HI GCNGC 6 cut(s) 9, 38, 45, 48, 51, 260
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 497
GluI GCNGC 6 cut(s) 9, 38, 45, 48, 51, 260
GsaI CCCAGC 1 cut(s) 428
HaeIII GGCC 3 cut(s) 214, 474, 641
HapII CCGG 9 cut(s) 54, 180, 215, 254, 275, 438, 450, 577, 598
HhaI GCGC 1 cut(s) 498
Hin1II CATG 4 cut(s) 152, 310, 341, 548
Hin6I GCGC 1 cut(s) 496
HinP1I GCGC 1 cut(s) 496
HinfI GANTC 2 cut(s) 21, 593
HpaII CCGG 9 cut(s) 54, 180, 215, 254, 275, 438, 450, 577, 598
HphI GGTGA 2 cut(s) 104, 301
Hpy166II GTNNAC 5 cut(s) 67, 121, 463, 469, 517
Hpy188I TCNGA 2 cut(s) 26, 302
Hpy188III TCNNGA 1 cut(s) 370
Hpy8I GTNNAC 5 cut(s) 67, 121, 463, 469, 517
HpyAV CCTTC 1 cut(s) 668
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4IV ACGT 1 cut(s) 465
HpyCH4V TGCA 7 cut(s) 102, 121, 148, 357, 380, 489, 517
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 50, 259, 495
HpySE526I ACGT 1 cut(s) 465
Hsp92II CATG 4 cut(s) 152, 310, 341, 548
HspAI GCGC 1 cut(s) 496
Ksp22I TGATCA 1 cut(s) 372
Kzo9I GATC 3 cut(s) 372, 415, 440
LmnI GCTCC 3 cut(s) 182, 553, 604
Lsp1109I GCAGC 3 cut(s) 20, 49, 246
MaeI CTAG 1 cut(s) 41
MaeII ACGT 1 cut(s) 465
MalI GATC 3 cut(s) 374, 417, 442
MboI GATC 3 cut(s) 372, 415, 440
MboII GAAGA 2 cut(s) 533, 599
MfeI CAATTG 1 cut(s) 381
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 3 cut(s) 123, 519, 643
MluCI AATT 2 cut(s) 269, 381
MlyI GAGTC 1 cut(s) 602
MmeI TCCRAC 1 cut(s) 582
MnlI CCTC 3 cut(s) 181, 279, 583
MseI TTAA 2 cut(s) 156, 667
MslI CAYNNNNRTG 2 cut(s) 126, 522
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 9 cut(s) 54, 180, 215, 254, 275, 438, 450, 577, 598
MspR9I CCNGG 5 cut(s) 180, 255, 332, 577, 598
MunI CAATTG 1 cut(s) 381
MvaI CCWGG 1 cut(s) 332
MwoI GCNNNNNNNGC 4 cut(s) 37, 50, 259, 495
NciI CCSGG 4 cut(s) 180, 255, 577, 598
NdeII GATC 3 cut(s) 372, 415, 440
NheI GCTAGC 1 cut(s) 40
NlaIII CATG 4 cut(s) 152, 310, 341, 548
NlaIV GGNNCC 3 cut(s) 213, 641, 642
NmeAIII GCCGAG 1 cut(s) 149
NspI RCATGY 1 cut(s) 152
PfeI GAWTC 1 cut(s) 21
PfoI TCCNGGA 1 cut(s) 178
PinAI ACCGGT 1 cut(s) 449
PkrI GCNGC 6 cut(s) 10, 39, 46, 49, 52, 261
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
Psp6I CCWGG 1 cut(s) 330
PspFI CCCAGC 1 cut(s) 424
PspGI CCWGG 1 cut(s) 330
PspN4I GGNNCC 3 cut(s) 213, 641, 642
PspOMI GGGCCC 1 cut(s) 639
PspPI GGNCC 3 cut(s) 212, 639, 640
PsuI RGATCY 1 cut(s) 415
RsaI GTAC 3 cut(s) 436, 470, 635
RsaNI GTAC 3 cut(s) 435, 469, 634
RseI CAYNNNNRTG 2 cut(s) 126, 522
SaqAI TTAA 2 cut(s) 156, 667
SatI GCNGC 6 cut(s) 9, 38, 45, 48, 51, 260
Sau3AI GATC 3 cut(s) 372, 415, 440
Sau96I GGNCC 3 cut(s) 212, 639, 640
ScaI AGTACT 1 cut(s) 635
SchI GAGTC 1 cut(s) 602
ScrFI CCNGG 5 cut(s) 180, 255, 332, 577, 598
SduI GDGCHC 3 cut(s) 123, 519, 643
SetI ASST 6 cut(s) 42, 80, 426, 468, 524, 564
SgrAI CRCCGGYG 1 cut(s) 53
SmiMI CAYNNNNRTG 2 cut(s) 126, 522
Sse9I AATT 2 cut(s) 269, 381
SsiI CCGC 7 cut(s) 11, 45, 48, 51, 227, 318, 649
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 5 cut(s) 178, 253, 330, 575, 596
TaaI ACNGT 1 cut(s) 92
TaiI ACGT 1 cut(s) 468
TaqI TCGA 1 cut(s) 558
TasI AATT 2 cut(s) 269, 381
TatI WGTACW 1 cut(s) 633
TauI GCSGC 3 cut(s) 47, 50, 53
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 2 cut(s) 156, 667
Tru9I TTAA 2 cut(s) 156, 667
TscAI CASTG 1 cut(s) 22
TseI GCWGC 3 cut(s) 8, 37, 259
TspDTI ATGAA 3 cut(s) 229, 326, 533
TspRI CASTG 1 cut(s) 22
VneI GTGCAC 2 cut(s) 119, 515
XceI RCATGY 1 cut(s) 152
XspI CTAG 1 cut(s) 41
ZrmI AGTACT 1 cut(s) 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.