Rh2DG692000

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0018714
Physical Location & Seq
Reverse (-)
90838983 .. 90840645
1663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG692000.1

Sequence Viewer

Length: 444 bp
ATGAGTGCTGATTGGGGGCCGGTCGTTGTTGCGGTGGTCTTGTTCATTCTCTTGTCGCCGGGGCTGCTATTCCAATTACCAGCAAGGACAAGGGTGATGGAGTTCGGAAACATGATGACAAGCGGGATTGCTATCCTGAGTTACAACAACAGAGTCTTCGAAAAGGCTAAAGAGATACAGAGACACACAGATCCAAAGCTGGGGGAGAGTACCGGATCATCAACCGGTGTAGATGTGGACGTGTACGGCCAAGACGAGAACGGCATAGAGCGCAAAAAAAATGAGTGTGTGCACAGCTATGGCTTTGCCGTTGGTCTTCATGCTCCCAAACTCGACCTGCTTGCCGTTTCCCGGCAACGAGAAGAGGAGTCCCGGTTGGAGCAGAATAAACAGCACAACCCCAATCAGTACTGGGCCCCAGTCCGCCATTTCCTTCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04216 map04136 map04138 map04140 map04216
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

147

Amino Acids

16.54

Weight (kDa)

6.51

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3339 PF11820 3 - 46 6.1e-21 Protein of unknown function (DUF3339)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018714)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27027 AT5G40980
malus_domestica MD09G1009300.v1.1 MD17G1001100.v1.1
prunus_persica Prupe.3G307600_v2.0.a1
pyrus_communis pycom111g00750
rosa_multiflora Rmu_sc0000998.1_g000004
rosa_samantha Rh2DG692000
rosa_wichuraiana Rw2G054680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 345
AciI CCGC 3 cut(s) 32, 123, 424
AclWI GGATC 2 cut(s) 185, 223
AcoI YGGCCR 1 cut(s) 247
AfaI GTAC 3 cut(s) 211, 245, 410
AfiI CCNNNNNNNGG 2 cut(s) 200, 351
AflIII ACRYGT 1 cut(s) 240
AgeI ACCGGT 1 cut(s) 224
AgsI TTSAA 1 cut(s) 437
AjiI CACGTC 1 cut(s) 241
AluBI AGCT 2 cut(s) 199, 297
AluI AGCT 2 cut(s) 199, 297
Alw21I GWGCWC 1 cut(s) 294
Alw26I GTCTC 1 cut(s) 175
Alw44I GTGCAC 1 cut(s) 290
AlwI GGATC 2 cut(s) 185, 223
AoxI GGCC 3 cut(s) 17, 247, 414
ApaI GGGCCC 1 cut(s) 418
ApaLI GTGCAC 1 cut(s) 290
ApeKI GCWGC 1 cut(s) 64
AsiGI ACCGGT 1 cut(s) 224
AspLEI GCGC 1 cut(s) 273
AspS9I GGNCC 3 cut(s) 17, 414, 415
AsuC2I CCSGG 3 cut(s) 60, 352, 373
AsuHPI GGTGA 1 cut(s) 106
AsuII TTCGAA 1 cut(s) 159
BaeGI GKGCMC 2 cut(s) 294, 418
BanII GRGCYC 1 cut(s) 418
BbsI GAAGAC 2 cut(s) 148, 308
Bbv12I GWGCWC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 51
BccI CCATC 1 cut(s) 91
BceAI ACGGC 4 cut(s) 262, 277, 293, 329
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BcnI CCSGG 3 cut(s) 60, 352, 373
BcoDI GTCTC 1 cut(s) 175
BfuAI ACCTGC 1 cut(s) 345
BisI GCNGC 1 cut(s) 65
BlsI GCNGC 1 cut(s) 66
BmcAI AGTACT 1 cut(s) 410
Bme1390I CCNGG 3 cut(s) 60, 352, 373
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 3 cut(s) 17, 414, 415
BmiI GGNNCC 3 cut(s) 18, 416, 417
BmrFI CCNGG 3 cut(s) 60, 352, 373
BmrI ACTGGG 2 cut(s) 413, 421
BmuI ACTGGG 2 cut(s) 413, 421
BpiI GAAGAC 2 cut(s) 148, 308
Bpu14I TTCGAA 1 cut(s) 159
BpuMI CCSGG 3 cut(s) 60, 352, 373
BsaBI GATNNNNATC 1 cut(s) 131
BsaJI CCNNGG 1 cut(s) 59
BsaWI WCCGGW 2 cut(s) 212, 224
BsaXI ACNNNNNCTCC 2 cut(s) 359, 389
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 351
Bse118I RCCGGY 2 cut(s) 19, 224
Bse1I ACTGG 2 cut(s) 416, 419
Bse8I GATNNNNATC 1 cut(s) 131
BseDI CCNNGG 1 cut(s) 59
BseJI GATNNNNATC 1 cut(s) 131
BseLI CCNNNNNNNGG 2 cut(s) 200, 351
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 2 cut(s) 416, 419
BseRI GAGGAG 1 cut(s) 380
BseSI GKGCMC 2 cut(s) 294, 418
BseXI GCAGC 1 cut(s) 51
BseYI CCCAGC 1 cut(s) 199
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 3 cut(s) 19, 249, 416
BshTI ACCGGT 1 cut(s) 224
BsiEI CGRYCG 1 cut(s) 24
BsiHKAI GWGCWC 1 cut(s) 294
BsiSI CCGG 6 cut(s) 20, 59, 213, 225, 352, 373
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 2 cut(s) 200, 351
BsmAI GTCTC 1 cut(s) 175
BsmFI GGGAC 1 cut(s) 355
BsnI GGCC 3 cut(s) 19, 249, 416
Bsp119I TTCGAA 1 cut(s) 159
Bsp120I GGGCCC 1 cut(s) 414
Bsp1286I GDGCHC 2 cut(s) 294, 418
Bsp143I GATC 2 cut(s) 190, 215
BspACI CCGC 3 cut(s) 32, 123, 424
BspANI GGCC 3 cut(s) 19, 249, 416
BspCNI CTCAG 1 cut(s) 129
BspLI GGNNCC 3 cut(s) 18, 416, 417
BspMI ACCTGC 1 cut(s) 345
BspPI GGATC 2 cut(s) 185, 223
BspT104I TTCGAA 1 cut(s) 159
BsrFI RCCGGY 2 cut(s) 19, 224
BsrI ACTGG 2 cut(s) 416, 419
BssAI RCCGGY 2 cut(s) 19, 224
BssECI CCNNGG 1 cut(s) 59
BssMI GATC 2 cut(s) 190, 215
Bst6I CTCTTC 1 cut(s) 357
BstBI TTCGAA 1 cut(s) 159
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 1 cut(s) 137
BstHHI GCGC 1 cut(s) 273
BstKTI GATC 2 cut(s) 193, 218
BstMAI GTCTC 1 cut(s) 175
BstMBI GATC 2 cut(s) 190, 215
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 2 cut(s) 64, 270
BstSCI CCNGG 3 cut(s) 58, 350, 371
BstSLI GKGCMC 2 cut(s) 294, 418
BstV1I GCAGC 1 cut(s) 51
BstV2I GAAGAC 2 cut(s) 148, 308
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BsuRI GGCC 3 cut(s) 19, 249, 416
BtrI CACGTC 1 cut(s) 241
BveI ACCTGC 1 cut(s) 345
Cac8I GCNNGC 1 cut(s) 342
CfoI GCGC 1 cut(s) 273
Cfr10I RCCGGY 2 cut(s) 19, 224
Cfr13I GGNCC 3 cut(s) 17, 414, 415
Csp6I GTAC 3 cut(s) 210, 244, 409
CspAI ACCGGT 1 cut(s) 224
CviAII CATG 2 cut(s) 112, 320
CviJI RGCY 8 cut(s) 19, 64, 167, 199, 249, 297, 303, 416
CviKI_1 RGCY 8 cut(s) 19, 64, 167, 199, 249, 297, 303, 416
CviQI GTAC 3 cut(s) 210, 244, 409
DdeI CTNAG 1 cut(s) 137
DpnI GATC 2 cut(s) 192, 217
DpnII GATC 2 cut(s) 190, 215
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
EciI GGCGGA 1 cut(s) 413
Eco24I GRGCYC 1 cut(s) 418
EcoO109I RGGNCCY 1 cut(s) 415
EcoT38I GRGCYC 1 cut(s) 418
FaeI CATG 2 cut(s) 115, 323
FaiI YATR 4 cut(s) 113, 266, 300, 321
FaqI GGGAC 1 cut(s) 355
FatI CATG 2 cut(s) 111, 319
FauI CCCGC 1 cut(s) 116
Fnu4HI GCNGC 1 cut(s) 65
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 1 cut(s) 65
GlaI GCGC 1 cut(s) 272
GluI GCNGC 1 cut(s) 65
GsaI CCCAGC 1 cut(s) 203
HaeIII GGCC 3 cut(s) 19, 249, 416
HapII CCGG 6 cut(s) 20, 59, 213, 225, 352, 373
HhaI GCGC 1 cut(s) 273
Hin1II CATG 2 cut(s) 115, 323
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HinfI GANTC 2 cut(s) 153, 368
HpaII CCGG 6 cut(s) 20, 59, 213, 225, 352, 373
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 3 cut(s) 238, 244, 292
Hpy188I TCNGA 1 cut(s) 107
Hpy188III TCNNGA 1 cut(s) 136
Hpy8I GTNNAC 3 cut(s) 238, 244, 292
HpyAV CCTTC 1 cut(s) 443
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 1 cut(s) 292
HpyF10VI GCNNNNNNNGC 2 cut(s) 64, 270
HpyF3I CTNAG 1 cut(s) 137
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 2 cut(s) 115, 323
HspAI GCGC 1 cut(s) 271
Kzo9I GATC 2 cut(s) 190, 215
LmnI GCTCC 2 cut(s) 328, 379
Lsp1109I GCAGC 1 cut(s) 51
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 140
MalI GATC 2 cut(s) 192, 217
MboI GATC 2 cut(s) 190, 215
MboII GAAGA 3 cut(s) 148, 308, 374
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 2 cut(s) 294, 418
MluCI AATT 1 cut(s) 74
MlyI GAGTC 2 cut(s) 162, 377
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 1 cut(s) 358
MseI TTAA 1 cut(s) 442
MslI CAYNNNNRTG 1 cut(s) 297
MspI CCGG 6 cut(s) 20, 59, 213, 225, 352, 373
MspR9I CCNGG 3 cut(s) 60, 352, 373
MwoI GCNNNNNNNGC 2 cut(s) 64, 270
NciI CCSGG 3 cut(s) 60, 352, 373
NdeII GATC 2 cut(s) 190, 215
NlaIII CATG 2 cut(s) 115, 323
NlaIV GGNNCC 3 cut(s) 18, 416, 417
NspV TTCGAA 1 cut(s) 159
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PinAI ACCGGT 1 cut(s) 224
PkrI GCNGC 1 cut(s) 66
PleI GAGTC 2 cut(s) 161, 376
PpsI GAGTC 2 cut(s) 161, 376
PspFI CCCAGC 1 cut(s) 199
PspN4I GGNNCC 3 cut(s) 18, 416, 417
PspOMI GGGCCC 1 cut(s) 414
PspPI GGNCC 3 cut(s) 17, 414, 415
PsuI RGATCY 1 cut(s) 190
RsaI GTAC 3 cut(s) 211, 245, 410
RsaNI GTAC 3 cut(s) 210, 244, 409
RseI CAYNNNNRTG 1 cut(s) 297
SaqAI TTAA 1 cut(s) 442
SatI GCNGC 1 cut(s) 65
Sau3AI GATC 2 cut(s) 190, 215
Sau96I GGNCC 3 cut(s) 17, 414, 415
ScaI AGTACT 1 cut(s) 410
SchI GAGTC 2 cut(s) 162, 377
ScrFI CCNGG 3 cut(s) 60, 352, 373
SduI GDGCHC 2 cut(s) 294, 418
SetI ASST 4 cut(s) 201, 243, 299, 339
SfuI TTCGAA 1 cut(s) 159
SmiMI CAYNNNNRTG 1 cut(s) 297
Sse9I AATT 1 cut(s) 74
SsiI CCGC 3 cut(s) 32, 123, 424
StyD4I CCNGG 3 cut(s) 58, 350, 371
TaiI ACGT 1 cut(s) 243
TaqI TCGA 2 cut(s) 159, 333
TasI AATT 1 cut(s) 74
TatI WGTACW 1 cut(s) 408
Tru1I TTAA 1 cut(s) 442
Tru9I TTAA 1 cut(s) 442
TseI GCWGC 1 cut(s) 64
TspDTI ATGAA 2 cut(s) 34, 308
VneI GTGCAC 1 cut(s) 290
ZrmI AGTACT 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.