Rmu_sc0001162.1_g000001

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001162.1
OG0016400
Physical Location & Seq
Forward (+)
8 .. 3904
3897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001162.1_g000001.1.cds

Sequence Viewer

Length: 1461 bp
atgaaacctgggaagctgaaaccaatggtgctggtccgtgatccgttaaggaattgggagctaaaatgggtaacgacggtggagaacccggaaaaaccaggacaaatcctatgggtgaaatatatggaagggctgtcagaggacgatttagatatacctccgttgagtgagaagatgaaagaaatgaaaaaaaccgcttctcggatcctttcaactcgtgggactaaaaccgaccagtgggtttgtgggatttgtaatcagtgggatgaccactggcctccaacgtgcccgtacattgatgatcctacagtaacccacttcacaacggcacaactcatacaggctcaaggtagcaatgcagaagaaccaaagtataatgctgcagactgtttggctcaagcctcgaaaaagagtggtaacgtgtatagccattggcaatgtaggatttgtggtaagatagatgaccactggcctccaatgtgcccgtacaaatgtatctatcccgactttgctgaatacagtgatagcgcgtctgctactggcaagagtacattccaaatgcctgaaagctttaccaaaggaggtgatgctatgttttgtgggatttgtggtgcgagggaccacatccctaaaacatgctactacaagcgttgtgtcaaattattcactggtacacctcctcctgtcgagactattgatgttcgtccaagctcaccagaaaggaaattcacttggtcgacggacttccttgttgtgccagaggagtttttgcatacgtcaatgtaccaatgggtgaagcccagcggtgatgtggatgaggaattctttgcttccccggcggagatggttgaatatatcatggagacaaacaagaagagtaaaccaacaggtagtatggcagaaaaaccagtggttgttggaggtgcttttacttctccacgacatcctcttgagtactgtaggatttgttgcaagtacattgaccactcagatataacttgtccgtacctggtatatgtcccacctggtgcaactgttggccctggctgtctagtagtttgtagtgtgtgtggttccgagaaatgtgacgaatgtcgggcccaacgtgttgctcccctgtattgtctgatttgtggtactaagtttgaccacctcgagagagattgcccactcaagacttgtgcaatctctgttcgtcttgatgataaaaattaccccacttggcattttctgatgcagcactaccttcgtggtcttggattgctcaagtttgtagatggttcttattcttgccctccacagtttcagagggccgattatgattctctctctcttgacaacactgaagcttatgaagagtggctgcaacaagatagttatgtgatttgttcgctttctgctgatgccacttactctggttattggatgttagacatcgatggaggtatggctcacccttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

486

Amino Acids

54.95

Weight (kDa)

5.46

Isoelectric Point (pI)

34.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016400)

Species Orthologous Gene IDs
malus_domestica MD10G1001800.v1.1
pyrus_communis pycom10g00170
rosa_laevigata RLG00000007703 RLG00000007704
rosa_multiflora Rmu_sc0001162.1_g000001
rosa_rugosa Rorug04G0169600 Rorug04G0175900
rosa_samantha Rh4AG230300 Rh4AG234900 Rh4BG233200 Rh4CG249800 Rh4DG229000 Rh4DG233800
rosa_wichuraiana Rw4G019940 Rw4G020350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 737
AccII CGCG 1 cut(s) 530
AciI CCGC 3 cut(s) 195, 804, 839
AclWI GGATC 4 cut(s) 35, 199, 212, 296
AcsI RAATTY 2 cut(s) 725, 821
AcuI CTGAAG 1 cut(s) 1365
AdeI CACNNNGTG 1 cut(s) 1028
AfaI GTAC 9 cut(s) 293, 488, 550, 673, 785, 956, 977, 1007, 1138
AfiI CCNNNNNNNGG 2 cut(s) 201, 237
AflIII ACRYGT 2 cut(s) 420, 1105
AgsI TTSAA 2 cut(s) 213, 851
AjnI CCWGG 5 cut(s) 7, 97, 1008, 1024, 1042
AluBI AGCT 5 cut(s) 16, 61, 570, 711, 1349
AluI AGCT 5 cut(s) 16, 61, 570, 711, 1349
Alw26I GTCTC 2 cut(s) 683, 857
AlwI GGATC 4 cut(s) 35, 199, 212, 296
Ama87I CYCGRG 1 cut(s) 1154
AoxI GGCC 5 cut(s) 275, 470, 1039, 1098, 1311
ApaI GGGCCC 1 cut(s) 1102
ApeKI GCWGC 3 cut(s) 380, 1237, 1363
ApoI RAATTY 2 cut(s) 725, 821
AspLEI GCGC 1 cut(s) 530
AspS9I GGNCC 6 cut(s) 34, 619, 1040, 1098, 1099, 1311
AsuC2I CCSGG 2 cut(s) 89, 836
AsuHPI GGTGA 6 cut(s) 127, 596, 705, 805, 818, 1445
AvaI CYCGRG 1 cut(s) 1154
AvaII GGWCC 2 cut(s) 34, 619
BaeGI GKGCMC 3 cut(s) 290, 485, 1102
BaeI ACNNNNGTAYC 2 cut(s) 478, 511
BamHI GGATCC 1 cut(s) 204
BanII GRGCYC 1 cut(s) 1102
BauI CACGAG 1 cut(s) 216
BbvI GCAGC 3 cut(s) 367, 1249, 1350
BccI CCATC 3 cut(s) 838, 1271, 1433
BceAI ACGGC 1 cut(s) 342
BcgI CGANNNNNNTGC 2 cut(s) 1229, 1263
BciT130I CCWGG 5 cut(s) 9, 99, 1010, 1026, 1044
BcnI CCSGG 2 cut(s) 89, 836
BcoDI GTCTC 2 cut(s) 683, 857
BfaI CTAG 1 cut(s) 1052
BfmI CTRYAG 3 cut(s) 306, 381, 958
BisI GCNGC 3 cut(s) 381, 1238, 1364
BlsI GCNGC 3 cut(s) 382, 1239, 1365
BmcAI AGTACT 1 cut(s) 956
Bme1390I CCNGG 7 cut(s) 9, 89, 99, 836, 1010, 1026, 1044
Bme18I GGWCC 2 cut(s) 34, 619
BmeT110I CYCGRG 1 cut(s) 1154
BmgT120I GGNCC 6 cut(s) 34, 619, 1040, 1098, 1099, 1311
BmiI GGNNCC 4 cut(s) 206, 620, 1075, 1100
BmrFI CCNGG 7 cut(s) 9, 89, 99, 836, 1010, 1026, 1044
BmsI GCATC 3 cut(s) 577, 1224, 1393
BoxI GACNNNNGTC 1 cut(s) 1092
BpuEI CTTGAG 5 cut(s) 330, 381, 971, 1157, 1250
BpuMI CCSGG 2 cut(s) 89, 836
Bsa29I ATCGAT 1 cut(s) 1437
BsaJI CCNNGG 3 cut(s) 8, 834, 1042
BsaXI ACNNNNNCTCC 2 cut(s) 664, 694
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 237
Bse1I ACTGG 6 cut(s) 235, 278, 473, 544, 673, 908
Bse3DI GCAATG 2 cut(s) 361, 443
BseBI CCWGG 5 cut(s) 9, 99, 1010, 1026, 1044
BseCI ATCGAT 1 cut(s) 1437
BseDI CCNNGG 3 cut(s) 8, 834, 1042
BseGI GGATG 5 cut(s) 271, 624, 820, 943, 1431
BseLI CCNNNNNNNGG 2 cut(s) 201, 237
BseMI GCAATG 2 cut(s) 361, 443
BseMII CTCAG 1 cut(s) 1002
BseNI ACTGG 6 cut(s) 235, 278, 473, 544, 673, 908
BseRI GAGGAG 2 cut(s) 669, 776
BseSI GKGCMC 3 cut(s) 290, 485, 1102
BseXI GCAGC 3 cut(s) 367, 1249, 1350
BseYI CCCAGC 1 cut(s) 800
Bsh1236I CGCG 1 cut(s) 530
BshFI GGCC 5 cut(s) 277, 472, 1041, 1100, 1313
BshVI ATCGAT 1 cut(s) 1437
BsiHKCI CYCGRG 1 cut(s) 1154
BsiSI CCGG 2 cut(s) 89, 836
BslFI GGGAC 3 cut(s) 235, 632, 1004
BslI CCNNNNNNNGG 2 cut(s) 201, 237
BsmAI GTCTC 2 cut(s) 683, 857
BsmFI GGGAC 3 cut(s) 235, 632, 1004
BsnI GGCC 5 cut(s) 277, 472, 1041, 1100, 1313
BsoBI CYCGRG 1 cut(s) 1154
Bsp120I GGGCCC 1 cut(s) 1098
Bsp1286I GDGCHC 3 cut(s) 290, 485, 1102
Bsp143I GATC 3 cut(s) 40, 204, 301
BspACI CCGC 3 cut(s) 195, 804, 839
BspANI GGCC 5 cut(s) 277, 472, 1041, 1100, 1313
BspCNI CTCAG 1 cut(s) 1001
BspDI ATCGAT 1 cut(s) 1437
BspFNI CGCG 1 cut(s) 530
BspLI GGNNCC 4 cut(s) 206, 620, 1075, 1100
BspMAI CTGCAG 1 cut(s) 385
BspPI GGATC 4 cut(s) 35, 199, 212, 296
BsrDI GCAATG 2 cut(s) 361, 443
BsrI ACTGG 6 cut(s) 235, 278, 473, 544, 673, 908
BssECI CCNNGG 3 cut(s) 8, 834, 1042
BssMI GATC 3 cut(s) 40, 204, 301
BssSI CACGAG 1 cut(s) 216
Bst2BI CACGAG 1 cut(s) 216
Bst2UI CCWGG 5 cut(s) 9, 99, 1010, 1026, 1044
Bst4CI ACNGT 7 cut(s) 79, 310, 389, 521, 959, 1036, 1302
Bst6I CTCTTC 2 cut(s) 869, 1350
BstDEI CTNAG 2 cut(s) 988, 1140
BstF5I GGATG 5 cut(s) 271, 624, 820, 943, 1431
BstFNI CGCG 1 cut(s) 530
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 3 cut(s) 43, 207, 304
BstMAI GTCTC 2 cut(s) 683, 857
BstMBI GATC 3 cut(s) 40, 204, 301
BstMWI GCNNNNNNNGC 1 cut(s) 836
BstNI CCWGG 5 cut(s) 9, 99, 1010, 1026, 1044
BstNSI RCATGY 1 cut(s) 639
BstPAI GACNNNNGTC 1 cut(s) 1092
BstSCI CCNGG 7 cut(s) 7, 87, 97, 834, 1008, 1024, 1042
BstSFI CTRYAG 3 cut(s) 306, 381, 958
BstSLI GKGCMC 3 cut(s) 290, 485, 1102
BstUI CGCG 1 cut(s) 530
BstV1I GCAGC 3 cut(s) 367, 1249, 1350
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
Bsu15I ATCGAT 1 cut(s) 1437
BsuRI GGCC 5 cut(s) 277, 472, 1041, 1100, 1313
BsuTUI ATCGAT 1 cut(s) 1437
BtsCI GGATG 5 cut(s) 271, 624, 820, 943, 1431
BtsIMutI CAGTG 8 cut(s) 242, 266, 271, 466, 526, 666, 915, 1341
CfoI GCGC 1 cut(s) 530
Cfr13I GGNCC 6 cut(s) 34, 619, 1040, 1098, 1099, 1311
ClaI ATCGAT 1 cut(s) 1437
CseI GACGC 1 cut(s) 519
CsiI ACCWGGT 2 cut(s) 1008, 1024
Csp6I GTAC 9 cut(s) 292, 487, 549, 672, 784, 955, 976, 1006, 1137
CviAII CATG 2 cut(s) 636, 859
CviQI GTAC 9 cut(s) 292, 487, 549, 672, 784, 955, 976, 1006, 1137
DdeI CTNAG 2 cut(s) 988, 1140
DpnI GATC 3 cut(s) 42, 206, 303
DpnII GATC 3 cut(s) 40, 204, 301
DraIII CACNNNGTG 1 cut(s) 1028
Eam1104I CTCTTC 2 cut(s) 869, 1350
EarI CTCTTC 2 cut(s) 869, 1350
EciI GGCGGA 1 cut(s) 854
Eco24I GRGCYC 1 cut(s) 1102
Eco47I GGWCC 2 cut(s) 34, 619
Eco57I CTGAAG 1 cut(s) 1365
Eco88I CYCGRG 1 cut(s) 1154
EcoRI GAATTC 1 cut(s) 821
EcoRII CCWGG 5 cut(s) 7, 97, 1008, 1024, 1042
EcoT38I GRGCYC 1 cut(s) 1102
FaeI CATG 2 cut(s) 639, 862
FaqI GGGAC 3 cut(s) 235, 632, 1004
FatI CATG 2 cut(s) 635, 858
FblI GTMKAC 1 cut(s) 737
Fnu4HI GCNGC 3 cut(s) 381, 1238, 1364
FokI GGATG 5 cut(s) 278, 611, 827, 930, 1438
FriOI GRGCYC 1 cut(s) 1102
Fsp4HI GCNGC 3 cut(s) 381, 1238, 1364
FspBI CTAG 1 cut(s) 1052
GlaI GCGC 1 cut(s) 529
GluI GCNGC 3 cut(s) 381, 1238, 1364
GsaI CCCAGC 1 cut(s) 804
HaeIII GGCC 5 cut(s) 277, 472, 1041, 1100, 1313
HapII CCGG 2 cut(s) 89, 836
HgaI GACGC 1 cut(s) 519
HhaI GCGC 1 cut(s) 530
Hin1II CATG 2 cut(s) 639, 862
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HincII GTYRAC 1 cut(s) 738
HindII GTYRAC 1 cut(s) 738
HindIII AAGCTT 2 cut(s) 568, 1347
HinfI GANTC 1 cut(s) 1322
HpaII CCGG 2 cut(s) 89, 836
HphI GGTGA 6 cut(s) 127, 596, 705, 805, 818, 1445
Hpy166II GTNNAC 3 cut(s) 674, 738, 881
Hpy188I TCNGA 7 cut(s) 139, 204, 991, 1078, 1128, 1233, 1308
Hpy188III TCNNGA 7 cut(s) 503, 688, 950, 1156, 1174, 1199, 1334
Hpy8I GTNNAC 3 cut(s) 674, 738, 881
Hpy99I CGWCG 2 cut(s) 79, 742
HpyAV CCTTC 2 cut(s) 122, 1256
HpyCH4III ACNGT 7 cut(s) 79, 310, 389, 521, 959, 1036, 1302
HpyCH4IV ACGT 4 cut(s) 284, 420, 776, 1105
HpyCH4V TGCA 8 cut(s) 359, 383, 772, 972, 1031, 1184, 1237, 1366
HpyF10VI GCNNNNNNNGC 1 cut(s) 836
HpyF3I CTNAG 2 cut(s) 988, 1140
HpySE526I ACGT 4 cut(s) 284, 420, 776, 1105
Hsp92II CATG 2 cut(s) 639, 862
HspAI GCGC 1 cut(s) 528
Kzo9I GATC 3 cut(s) 40, 204, 301
LmnI GCTCC 2 cut(s) 58, 1117
Lsp1109I GCAGC 3 cut(s) 367, 1249, 1350
LweI GCATC 3 cut(s) 577, 1224, 1393
MabI ACCWGGT 2 cut(s) 1008, 1024
MaeI CTAG 1 cut(s) 1052
MaeII ACGT 4 cut(s) 284, 420, 776, 1105
MaeIII GTNAC 4 cut(s) 70, 310, 416, 1085
MalI GATC 3 cut(s) 42, 206, 303
MboI GATC 3 cut(s) 40, 204, 301
MboII GAAGA 4 cut(s) 184, 374, 886, 1367
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 3 cut(s) 290, 485, 1102
MluCI AATT 5 cut(s) 52, 659, 725, 821, 1210
MmeI TCCRAC 2 cut(s) 305, 898
MseI TTAA 2 cut(s) 47, 1459
MspA1I CMGCKG 1 cut(s) 804
MspI CCGG 2 cut(s) 89, 836
MspR9I CCNGG 7 cut(s) 9, 89, 99, 836, 1010, 1026, 1044
MvaI CCWGG 5 cut(s) 9, 99, 1010, 1026, 1044
MvnI CGCG 1 cut(s) 530
MwoI GCNNNNNNNGC 1 cut(s) 836
NciI CCSGG 2 cut(s) 89, 836
NdeII GATC 3 cut(s) 40, 204, 301
NlaIII CATG 2 cut(s) 639, 862
NlaIV GGNNCC 4 cut(s) 206, 620, 1075, 1100
NmuCI GTSAC 1 cut(s) 1085
NspI RCATGY 1 cut(s) 639
PaeR7I CTCGAG 1 cut(s) 1154
PcsI WCGNNNNNNNCGW 1 cut(s) 1102
PfeI GAWTC 1 cut(s) 1322
PkrI GCNGC 3 cut(s) 382, 1239, 1365
PshAI GACNNNNGTC 1 cut(s) 1092
Psp6I CCWGG 5 cut(s) 7, 97, 1008, 1024, 1042
PspFI CCCAGC 1 cut(s) 800
PspGI CCWGG 5 cut(s) 7, 97, 1008, 1024, 1042
PspN4I GGNNCC 4 cut(s) 206, 620, 1075, 1100
PspOMI GGGCCC 1 cut(s) 1098
PspPI GGNCC 6 cut(s) 34, 619, 1040, 1098, 1099, 1311
PstI CTGCAG 1 cut(s) 385
PsuI RGATCY 1 cut(s) 204
RsaI GTAC 9 cut(s) 293, 488, 550, 673, 785, 956, 977, 1007, 1138
RsaNI GTAC 9 cut(s) 292, 487, 549, 672, 784, 955, 976, 1006, 1137
SalI GTCGAC 1 cut(s) 736
SaqAI TTAA 2 cut(s) 47, 1459
SatI GCNGC 3 cut(s) 381, 1238, 1364
Sau3AI GATC 3 cut(s) 40, 204, 301
Sau96I GGNCC 6 cut(s) 34, 619, 1040, 1098, 1099, 1311
ScaI AGTACT 1 cut(s) 956
ScrFI CCNGG 7 cut(s) 9, 89, 99, 836, 1010, 1026, 1044
SduI GDGCHC 3 cut(s) 290, 485, 1102
SexAI ACCWGGT 2 cut(s) 1008, 1024
SfaNI GCATC 3 cut(s) 577, 1224, 1393
SfcI CTRYAG 3 cut(s) 306, 381, 958
Sfr274I CTCGAG 1 cut(s) 1154
SinI GGWCC 2 cut(s) 34, 619
SlaI CTCGAG 1 cut(s) 1154
SmlI CTYRAG 6 cut(s) 345, 396, 950, 1154, 1172, 1265
SmoI CTYRAG 6 cut(s) 345, 396, 950, 1154, 1172, 1265
Sse9I AATT 5 cut(s) 52, 659, 725, 821, 1210
SsiI CCGC 3 cut(s) 195, 804, 839
SspMI CTAG 1 cut(s) 1052
StyD4I CCNGG 7 cut(s) 7, 87, 97, 834, 1008, 1024, 1042
TaaI ACNGT 7 cut(s) 79, 310, 389, 521, 959, 1036, 1302
TaiI ACGT 4 cut(s) 287, 423, 779, 1108
TaqI TCGA 5 cut(s) 404, 687, 737, 1155, 1437
TasI AATT 5 cut(s) 52, 659, 725, 821, 1210
TatI WGTACW 3 cut(s) 548, 954, 975
TfiI GAWTC 1 cut(s) 1322
Tru1I TTAA 2 cut(s) 47, 1459
Tru9I TTAA 2 cut(s) 47, 1459
TscAI CASTG 8 cut(s) 242, 266, 278, 473, 526, 673, 915, 1348
TseFI GTSAC 1 cut(s) 1085
TseI GCWGC 3 cut(s) 380, 1237, 1363
Tsp45I GTSAC 1 cut(s) 1085
TspDTI ATGAA 4 cut(s) 17, 191, 200, 1368
TspGWI ACGGA 5 cut(s) 26, 33, 150, 755, 993
TspRI CASTG 8 cut(s) 242, 266, 278, 473, 526, 673, 915, 1348
VpaK11BI GGWCC 2 cut(s) 34, 619
XapI RAATTY 2 cut(s) 725, 821
XceI RCATGY 1 cut(s) 639
XcmI CCANNNNNNNNNTGG 2 cut(s) 242, 808
XhoI CTCGAG 1 cut(s) 1154
XmiI GTMKAC 1 cut(s) 737
XspI CTAG 1 cut(s) 1052
ZrmI AGTACT 1 cut(s) 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.