Rh4BG233200

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0016400
Physical Location & Seq
Forward (+)
39852929 .. 39855354
2426 bp
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UTR
Exon/CDS
Intron
Rh4BG233200.1

Sequence Viewer

Length: 1062 bp
ATGATCCGCATGAAACCTGGGAAGCTGAAACCAATGGTGCTGGTCTGTGATCCGTTAAAGAATTGGGAGCAAAAATGGGTAACGACGGTGGAGAACCCGGAAAAACCAGGACAAATCCTATGGGTGAAATACATGGAAGGGCTGTCGGAGGGCGATTTAGATGTACCACCGTTGAGTAGCAATGCAGAAGAACCAAAGGATAATGCTGCAGACTGTTTGGCTCAAGCCTCGAAAAAAAGTGGTAACGTGTATAGCCATTGGCAATGTAGGATTTGTGGTAAGATAGATGACCACTGGCCTCCAATGTGCCCGTACAAGTGTATCTATCCCGAATTTGCTGAATACAGTGATAGTGCGTCTGCTACTGGCAAGAGTACAGTCCAAATGCGTGAAAGCTTTACCAAAGGAGGTGATGTTATGTATTGTGGGATTTGTGGTGCGAGAGACCACATCCCTAAAATATGCTACTTCAAGCGTTTTGTCAAATTATTCACTGGTACACCTCCACCTGTCAAGACTATTGATGTTCGTCCAAGCTCACCAGAAAGCAAATTCACTTGGTCGACGGACTTCCTTGTTGTGCCAACGGAGTTTTTACGTACGTCAATGGACCAATGGGTGAAGCCTAGCGGTGATGTGGATGAGGAATTCTTTTCTTCCCCGGCGGAGATGGTTGAATATATCATGGAGACAAACAAGAAGAGTAAACCAACAGGTAGTATGGCAGAAAAACCAGTGGTTGTTGGAGGTGCGTTTACTTCTCCACGACGTCCTCTTGAGTACTGTAGGATTTGTTGCAAGTACATTGACCACTCAGATTTTACTTGTCCGTACCTGGAATATGTCCCACCTGGTGCAACTGTTGGCCCTGGCTGTCTAGTAGTTTGTAGTGTGTGTGGTTCCGAGAAATGTGACGAATGTCGGGCCCAACGTGTTGCTCCCATGTATTGTCTGAAGTGTGGTACTAAGTTTGACCACCTCGCGAGAGATTGCCCACTCAAGAACAGCTGCTGGTTCTCTTGTGAACTGGGTAGGTTCCCAGATAACAATCAATTAAACTAA

Protein Analysis

353

Amino Acids

39.68

Weight (kDa)

6.53

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016400)

Species Orthologous Gene IDs
malus_domestica MD10G1001800.v1.1
pyrus_communis pycom10g00170
rosa_laevigata RLG00000007703 RLG00000007704
rosa_multiflora Rmu_sc0001162.1_g000001
rosa_rugosa Rorug04G0169600 Rorug04G0175900
rosa_samantha Rh4AG230300 Rh4AG234900 Rh4BG233200 Rh4CG249800 Rh4DG229000 Rh4DG233800
rosa_wichuraiana Rw4G019940 Rw4G020350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 772
AccI GTMKAC 1 cut(s) 563
AccII CGCG 1 cut(s) 983
AciI CCGC 3 cut(s) 7, 630, 665
AclWI GGATC 1 cut(s) 44
AcsI RAATTY 3 cut(s) 332, 551, 647
AcuI CTGAAG 1 cut(s) 974
AcyI GRCGYC 1 cut(s) 769
AdeI CACNNNGTG 1 cut(s) 854
AfaI GTAC 9 cut(s) 165, 314, 376, 499, 601, 782, 803, 833, 964
AflIII ACRYGT 2 cut(s) 246, 931
AgsI TTSAA 2 cut(s) 472, 677
AjnI CCWGG 5 cut(s) 16, 106, 834, 850, 868
AluBI AGCT 4 cut(s) 25, 396, 537, 1008
AluI AGCT 4 cut(s) 25, 396, 537, 1008
Alw26I GTCTC 2 cut(s) 438, 683
AlwI GGATC 1 cut(s) 44
AlwNI CAGNNNCTG 1 cut(s) 1011
AoxI GGCC 3 cut(s) 296, 865, 924
ApaI GGGCCC 1 cut(s) 928
ApeKI GCWGC 2 cut(s) 206, 1008
ApoI RAATTY 3 cut(s) 332, 551, 647
AspS9I GGNCC 4 cut(s) 610, 866, 924, 925
AsuC2I CCSGG 2 cut(s) 98, 662
AsuHPI GGTGA 5 cut(s) 136, 422, 531, 631, 644
AvaII GGWCC 1 cut(s) 610
BaeGI GKGCMC 2 cut(s) 311, 928
BaeI ACNNNNGTAYC 2 cut(s) 304, 337
BanII GRGCYC 1 cut(s) 928
BbvI GCAGC 2 cut(s) 193, 995
BccI CCATC 1 cut(s) 664
BciT130I CCWGG 5 cut(s) 18, 108, 836, 852, 870
BcnI CCSGG 2 cut(s) 98, 662
BcoDI GTCTC 2 cut(s) 438, 683
BfaI CTAG 2 cut(s) 627, 878
BfmI CTRYAG 2 cut(s) 207, 784
BisI GCNGC 2 cut(s) 207, 1009
BlsI GCNGC 2 cut(s) 208, 1010
BmcAI AGTACT 1 cut(s) 782
Bme1390I CCNGG 7 cut(s) 18, 98, 108, 662, 836, 852, 870
Bme18I GGWCC 1 cut(s) 610
BmgT120I GGNCC 4 cut(s) 610, 866, 924, 925
BmiI GGNNCC 3 cut(s) 901, 926, 1037
BmrFI CCNGG 7 cut(s) 18, 98, 108, 662, 836, 852, 870
BmrI ACTGGG 1 cut(s) 1037
BmuI ACTGGG 1 cut(s) 1037
BoxI GACNNNNGTC 1 cut(s) 918
BpuEI CTTGAG 3 cut(s) 207, 797, 983
BpuMI CCSGG 2 cut(s) 98, 662
BsaAI YACGTR 1 cut(s) 599
BsaBI GATNNNNATC 1 cut(s) 1047
BsaHI GRCGYC 1 cut(s) 769
BsaI GGTCTC 1 cut(s) 438
BsaJI CCNNGG 3 cut(s) 17, 660, 868
BsaXI ACNNNNNCTCC 2 cut(s) 399, 429
Bse1I ACTGG 5 cut(s) 299, 370, 499, 734, 1032
Bse3DI GCAATG 2 cut(s) 187, 269
Bse8I GATNNNNATC 1 cut(s) 1047
BseBI CCWGG 5 cut(s) 18, 108, 836, 852, 870
BseDI CCNNGG 3 cut(s) 17, 660, 868
BseGI GGATG 2 cut(s) 450, 646
BseJI GATNNNNATC 1 cut(s) 1047
BseMI GCAATG 2 cut(s) 187, 269
BseMII CTCAG 1 cut(s) 828
BseNI ACTGG 5 cut(s) 299, 370, 499, 734, 1032
BseSI GKGCMC 2 cut(s) 311, 928
BseXI GCAGC 2 cut(s) 193, 995
Bsh1236I CGCG 1 cut(s) 983
BshFI GGCC 3 cut(s) 298, 867, 926
BsiSI CCGG 2 cut(s) 98, 662
BsiWI CGTACG 1 cut(s) 599
BslFI GGGAC 1 cut(s) 830
BsmAI GTCTC 2 cut(s) 438, 683
BsmFI GGGAC 1 cut(s) 830
BsnI GGCC 3 cut(s) 298, 867, 926
Bso31I GGTCTC 1 cut(s) 438
Bsp120I GGGCCC 1 cut(s) 924
Bsp1286I GDGCHC 2 cut(s) 311, 928
Bsp143I GATC 2 cut(s) 3, 49
Bsp68I TCGCGA 1 cut(s) 983
BspACI CCGC 3 cut(s) 7, 630, 665
BspANI GGCC 3 cut(s) 298, 867, 926
BspCNI CTCAG 1 cut(s) 827
BspFNI CGCG 1 cut(s) 983
BspLI GGNNCC 3 cut(s) 901, 926, 1037
BspMAI CTGCAG 1 cut(s) 211
BspPI GGATC 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 438
BsrDI GCAATG 2 cut(s) 187, 269
BsrI ACTGG 5 cut(s) 299, 370, 499, 734, 1032
BssECI CCNNGG 3 cut(s) 17, 660, 868
BssMI GATC 2 cut(s) 3, 49
BssNI GRCGYC 1 cut(s) 769
Bst2UI CCWGG 5 cut(s) 18, 108, 836, 852, 870
Bst4CI ACNGT 7 cut(s) 88, 171, 215, 347, 379, 785, 862
Bst6I CTCTTC 1 cut(s) 695
BstACI GRCGYC 1 cut(s) 769
BstBAI YACGTR 1 cut(s) 599
BstDEI CTNAG 2 cut(s) 814, 966
BstF5I GGATG 2 cut(s) 450, 646
BstFNI CGCG 1 cut(s) 983
BstKTI GATC 2 cut(s) 6, 52
BstMAI GTCTC 2 cut(s) 438, 683
BstMBI GATC 2 cut(s) 3, 49
BstNI CCWGG 5 cut(s) 18, 108, 836, 852, 870
BstPAI GACNNNNGTC 1 cut(s) 918
BstSCI CCNGG 7 cut(s) 16, 96, 106, 660, 834, 850, 868
BstSFI CTRYAG 2 cut(s) 207, 784
BstSLI GKGCMC 2 cut(s) 311, 928
BstSNI TACGTA 1 cut(s) 599
BstUI CGCG 1 cut(s) 983
BstV1I GCAGC 2 cut(s) 193, 995
BsuRI GGCC 3 cut(s) 298, 867, 926
BtsCI GGATG 2 cut(s) 450, 646
BtsIMutI CAGTG 4 cut(s) 292, 352, 492, 741
BtuMI TCGCGA 1 cut(s) 983
CaiI CAGNNNCTG 1 cut(s) 1011
Cfr13I GGNCC 4 cut(s) 610, 866, 924, 925
CseI GACGC 1 cut(s) 345
CsiI ACCWGGT 1 cut(s) 850
Csp6I GTAC 9 cut(s) 164, 313, 375, 498, 600, 781, 802, 832, 963
CviAII CATG 4 cut(s) 10, 133, 685, 943
CviQI GTAC 9 cut(s) 164, 313, 375, 498, 600, 781, 802, 832, 963
DdeI CTNAG 2 cut(s) 814, 966
DpnI GATC 2 cut(s) 5, 51
DpnII GATC 2 cut(s) 3, 49
DraIII CACNNNGTG 1 cut(s) 854
Eam1104I CTCTTC 1 cut(s) 695
EarI CTCTTC 1 cut(s) 695
EciI GGCGGA 1 cut(s) 680
Eco105I TACGTA 1 cut(s) 599
Eco24I GRGCYC 1 cut(s) 928
Eco31I GGTCTC 1 cut(s) 438
Eco47I GGWCC 1 cut(s) 610
Eco57I CTGAAG 1 cut(s) 974
EcoRI GAATTC 1 cut(s) 647
EcoRII CCWGG 5 cut(s) 16, 106, 834, 850, 868
EcoT38I GRGCYC 1 cut(s) 928
FaeI CATG 4 cut(s) 13, 136, 688, 946
FaqI GGGAC 1 cut(s) 830
FatI CATG 4 cut(s) 9, 132, 684, 942
FblI GTMKAC 1 cut(s) 563
Fnu4HI GCNGC 2 cut(s) 207, 1009
FokI GGATG 2 cut(s) 437, 653
FriOI GRGCYC 1 cut(s) 928
Fsp4HI GCNGC 2 cut(s) 207, 1009
FspBI CTAG 2 cut(s) 627, 878
GluI GCNGC 2 cut(s) 207, 1009
HaeIII GGCC 3 cut(s) 298, 867, 926
HapII CCGG 2 cut(s) 98, 662
HgaI GACGC 1 cut(s) 345
Hin1I GRCGYC 1 cut(s) 769
Hin1II CATG 4 cut(s) 13, 136, 688, 946
HincII GTYRAC 1 cut(s) 564
HindII GTYRAC 1 cut(s) 564
HindIII AAGCTT 1 cut(s) 394
HpaII CCGG 2 cut(s) 98, 662
HphI GGTGA 5 cut(s) 136, 422, 531, 631, 644
Hpy166II GTNNAC 5 cut(s) 500, 564, 707, 756, 1025
Hpy188I TCNGA 4 cut(s) 148, 817, 904, 954
Hpy188III TCNNGA 5 cut(s) 329, 514, 776, 982, 1000
Hpy8I GTNNAC 5 cut(s) 500, 564, 707, 756, 1025
Hpy99I CGWCG 3 cut(s) 88, 568, 771
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 7 cut(s) 88, 171, 215, 347, 379, 785, 862
HpyCH4IV ACGT 5 cut(s) 246, 598, 602, 769, 931
HpyCH4V TGCA 4 cut(s) 185, 209, 798, 857
HpyF3I CTNAG 2 cut(s) 814, 966
HpySE526I ACGT 5 cut(s) 246, 598, 602, 769, 931
Hsp92I GRCGYC 1 cut(s) 769
Hsp92II CATG 4 cut(s) 13, 136, 688, 946
Kzo9I GATC 2 cut(s) 3, 49
LmnI GCTCC 2 cut(s) 67, 943
Lsp1109I GCAGC 2 cut(s) 193, 995
MabI ACCWGGT 1 cut(s) 850
MaeI CTAG 2 cut(s) 627, 878
MaeII ACGT 5 cut(s) 246, 598, 602, 769, 931
MaeIII GTNAC 3 cut(s) 79, 242, 911
MalI GATC 2 cut(s) 5, 51
MboI GATC 2 cut(s) 3, 49
MboII GAAGA 3 cut(s) 200, 648, 712
MhlI GDGCHC 2 cut(s) 311, 928
MluCI AATT 6 cut(s) 61, 332, 485, 551, 647, 1052
MmeI TCCRAC 2 cut(s) 126, 724
MnlI CCTC 9 cut(s) 142, 238, 309, 401, 513, 637, 740, 783, 989
MseI TTAA 2 cut(s) 56, 1055
MspA1I CMGCKG 1 cut(s) 1008
MspI CCGG 2 cut(s) 98, 662
MspR9I CCNGG 7 cut(s) 18, 98, 108, 662, 836, 852, 870
MvaI CCWGG 5 cut(s) 18, 108, 836, 852, 870
MvnI CGCG 1 cut(s) 983
NciI CCSGG 2 cut(s) 98, 662
NdeII GATC 2 cut(s) 3, 49
NlaIII CATG 4 cut(s) 13, 136, 688, 946
NlaIV GGNNCC 3 cut(s) 901, 926, 1037
NmuCI GTSAC 1 cut(s) 911
NruI TCGCGA 1 cut(s) 983
PcsI WCGNNNNNNNCGW 1 cut(s) 928
Pfl23II CGTACG 1 cut(s) 599
PkrI GCNGC 2 cut(s) 208, 1010
Ppu21I YACGTR 1 cut(s) 599
PshAI GACNNNNGTC 1 cut(s) 918
Psp6I CCWGG 5 cut(s) 16, 106, 834, 850, 868
PspGI CCWGG 5 cut(s) 16, 106, 834, 850, 868
PspLI CGTACG 1 cut(s) 599
PspN4I GGNNCC 3 cut(s) 901, 926, 1037
PspOMI GGGCCC 1 cut(s) 924
PspPI GGNCC 4 cut(s) 610, 866, 924, 925
PstI CTGCAG 1 cut(s) 211
PstNI CAGNNNCTG 1 cut(s) 1011
PvuII CAGCTG 1 cut(s) 1008
RruI TCGCGA 1 cut(s) 983
RsaI GTAC 9 cut(s) 165, 314, 376, 499, 601, 782, 803, 833, 964
RsaNI GTAC 9 cut(s) 164, 313, 375, 498, 600, 781, 802, 832, 963
SalI GTCGAC 1 cut(s) 562
SaqAI TTAA 2 cut(s) 56, 1055
SatI GCNGC 2 cut(s) 207, 1009
Sau3AI GATC 2 cut(s) 3, 49
Sau96I GGNCC 4 cut(s) 610, 866, 924, 925
ScaI AGTACT 1 cut(s) 782
ScrFI CCNGG 7 cut(s) 18, 98, 108, 662, 836, 852, 870
SduI GDGCHC 2 cut(s) 311, 928
SexAI ACCWGGT 1 cut(s) 850
SfcI CTRYAG 2 cut(s) 207, 784
SinI GGWCC 1 cut(s) 610
SmlI CTYRAG 3 cut(s) 222, 776, 998
SmoI CTYRAG 3 cut(s) 222, 776, 998
SnaBI TACGTA 1 cut(s) 599
Sse9I AATT 6 cut(s) 61, 332, 485, 551, 647, 1052
SsiI CCGC 3 cut(s) 7, 630, 665
SspMI CTAG 2 cut(s) 627, 878
StyD4I CCNGG 7 cut(s) 16, 96, 106, 660, 834, 850, 868
TaaI ACNGT 7 cut(s) 88, 171, 215, 347, 379, 785, 862
TaiI ACGT 5 cut(s) 249, 601, 605, 772, 934
TaqI TCGA 2 cut(s) 230, 563
TasI AATT 6 cut(s) 61, 332, 485, 551, 647, 1052
TatI WGTACW 3 cut(s) 374, 780, 801
Tru1I TTAA 2 cut(s) 56, 1055
Tru9I TTAA 2 cut(s) 56, 1055
TscAI CASTG 4 cut(s) 299, 352, 499, 741
TseFI GTSAC 1 cut(s) 911
TseI GCWGC 2 cut(s) 206, 1008
Tsp45I GTSAC 1 cut(s) 911
TspDTI ATGAA 1 cut(s) 26
TspGWI ACGGA 4 cut(s) 42, 581, 602, 819
TspRI CASTG 4 cut(s) 299, 352, 499, 741
VpaK11BI GGWCC 1 cut(s) 610
XapI RAATTY 3 cut(s) 332, 551, 647
XmiI GTMKAC 1 cut(s) 563
XspI CTAG 2 cut(s) 627, 878
ZraI GACGTC 1 cut(s) 770
ZrmI AGTACT 1 cut(s) 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.