Rh4CG249800

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0016400
Physical Location & Seq
Forward (+)
50003857 .. 50006133
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG249800.1

Sequence Viewer

Length: 1197 bp
ATGATCCGCATGAAACCTGGGAAGCTGAAACCAATGGTGCTGGTCCGTGATCCGTTAAGGAATTGGGAGCTAAAATGGGTAACGACGGTGGAGAACCCAGAAAAACCAGGACAAATCCTATGGGTGAAATATATGGAAGCGCTGTCAGAGGACGATTTAGATATACCTCCGTTGAGTGAGAAGATGAAAGAAATGAAAAAAACCGCTTCTCGGATCCTTTCAACTCGTGGGACCAAAACCGACCAGTGGGTTTGTGGGATTTGTAATCAGTGGGATGACCACTGGCCTCCAACGTGCCCGTACATTGATGATCCTACACTAACCCACTTCACAACGGCACAACTCATACAGGCTCAAGGTAGCAATGCAGAAGAACCAAAGTATAATGCTGCAGACTGTTTGGCTCAAGCCTCGAAAAAGAGTGGTAACGTGTATAGCCATTGGCAATGTAGGATTTGTGGTAAGATAGATGACCACTGGCCTCCAATGTGCCGGTACAAATGTATCTATCCCGACTTTGCTGAATACAGTGATAGCGCGTCTGCTACTGGCAAGAGTTCATTCCAAATGCCTGAAAGCTTTACCAAAGGAGGTGATGTTATGTTTTGTGGGATTTGTGGTGCGAGGGACCACATCCCAAAAACATGCTACTACAAGCGTTGTGTCAAATTATTCACTGGTACACCTCCTCCTGTCGAGACTATTGATGTTCGTCCAAGCTCACCAGAAAGGAAATTCACTTGGTCAACGGACTTCCTTGTTGTGCCAGAGGAGTTTTTGCATACGTCAATGTACCAATGGGTGAAGCCTAGCGGTGATGTGGATGAGGAATTCTTTGCTTCCCCGGCGGAGATGGTTGAATATATCATGGAGACAAACAAGAAGAGTAAACCAACAGGTAGTATGGCAGAAAAACCAGTGGTTGTTGGAGGTGCTTTTACTTCTCCACGACATCCTCTTGAGTACTGCAGGATTTGTTGCAAGTACATTGACCACTCAGATATAACTTGTCCGTACCTGGTATATGTCCCACCTGGTGCAACTGTTGGCCCTGGCTGTCTAGTAGTTTGTAGTGTGTGTGGTTCCGAGAAATGTGACGAATGTCGGGCCCAACGTGTTGCTCCCCTGTATTGTCTGATTTGTGGTACTAAGTTTGACCACCTCGAGAGAGATTGCCCACTCAAGACTTGGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

398

Amino Acids

45.13

Weight (kDa)

6.33

Isoelectric Point (pI)

31.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016400)

Species Orthologous Gene IDs
malus_domestica MD10G1001800.v1.1
pyrus_communis pycom10g00170
rosa_laevigata RLG00000007703 RLG00000007704
rosa_multiflora Rmu_sc0001162.1_g000001
rosa_rugosa Rorug04G0169600 Rorug04G0175900
rosa_samantha Rh4AG230300 Rh4AG234900 Rh4BG233200 Rh4CG249800 Rh4DG229000 Rh4DG233800
rosa_wichuraiana Rw4G019940 Rw4G020350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 539
AciI CCGC 4 cut(s) 7, 204, 813, 848
AclWI GGATC 4 cut(s) 44, 208, 221, 305
AcsI RAATTY 2 cut(s) 734, 830
AdeI CACNNNGTG 1 cut(s) 1037
AfaI GTAC 9 cut(s) 302, 497, 682, 794, 965, 986, 1016, 1147, 1193
AfeI AGCGCT 1 cut(s) 141
AfiI CCNNNNNNNGG 2 cut(s) 210, 246
AflIII ACRYGT 2 cut(s) 429, 1114
AgsI TTSAA 2 cut(s) 222, 860
AjnI CCWGG 5 cut(s) 16, 106, 1017, 1033, 1051
AluBI AGCT 4 cut(s) 25, 70, 579, 720
AluI AGCT 4 cut(s) 25, 70, 579, 720
Alw26I GTCTC 2 cut(s) 692, 866
AlwI GGATC 4 cut(s) 44, 208, 221, 305
Ama87I CYCGRG 1 cut(s) 1163
Aor51HI AGCGCT 1 cut(s) 141
AoxI GGCC 4 cut(s) 284, 479, 1048, 1107
ApaI GGGCCC 1 cut(s) 1111
ApeKI GCWGC 1 cut(s) 389
ApoI RAATTY 2 cut(s) 734, 830
AspLEI GCGC 2 cut(s) 142, 539
AspS9I GGNCC 6 cut(s) 43, 231, 628, 1049, 1107, 1108
AsuC2I CCSGG 1 cut(s) 845
AsuHPI GGTGA 5 cut(s) 136, 605, 714, 814, 827
AvaI CYCGRG 1 cut(s) 1163
AvaII GGWCC 3 cut(s) 43, 231, 628
BaeGI GKGCMC 2 cut(s) 299, 1111
BaeI ACNNNNGTAYC 4 cut(s) 487, 487, 520, 520
BamHI GGATCC 1 cut(s) 213
BanII GRGCYC 1 cut(s) 1111
BauI CACGAG 1 cut(s) 225
BbvI GCAGC 1 cut(s) 376
BccI CCATC 1 cut(s) 847
BceAI ACGGC 1 cut(s) 351
BciT130I CCWGG 5 cut(s) 18, 108, 1019, 1035, 1053
BcnI CCSGG 1 cut(s) 845
BcoDI GTCTC 2 cut(s) 692, 866
BfaI CTAG 2 cut(s) 810, 1061
BfmI CTRYAG 2 cut(s) 390, 967
BfoI RGCGCY 1 cut(s) 143
BisI GCNGC 1 cut(s) 390
BlsI GCNGC 1 cut(s) 391
BmcAI AGTACT 1 cut(s) 965
Bme1390I CCNGG 6 cut(s) 18, 108, 845, 1019, 1035, 1053
Bme18I GGWCC 3 cut(s) 43, 231, 628
BmeT110I CYCGRG 1 cut(s) 1163
BmgT120I GGNCC 6 cut(s) 43, 231, 628, 1049, 1107, 1108
BmiI GGNNCC 5 cut(s) 215, 232, 629, 1084, 1109
BmrFI CCNGG 6 cut(s) 18, 108, 845, 1019, 1035, 1053
BoxI GACNNNNGTC 1 cut(s) 1101
BpuEI CTTGAG 4 cut(s) 339, 390, 980, 1166
BpuMI CCSGG 1 cut(s) 845
BsaJI CCNNGG 3 cut(s) 17, 843, 1051
BsaXI ACNNNNNCTCC 4 cut(s) 582, 612, 673, 703
Bsc4I CCNNNNNNNGG 2 cut(s) 210, 246
Bse118I RCCGGY 1 cut(s) 492
Bse1I ACTGG 6 cut(s) 244, 287, 482, 553, 682, 917
Bse3DI GCAATG 2 cut(s) 370, 452
BseBI CCWGG 5 cut(s) 18, 108, 1019, 1035, 1053
BseDI CCNNGG 3 cut(s) 17, 843, 1051
BseGI GGATG 4 cut(s) 280, 633, 829, 952
BseLI CCNNNNNNNGG 2 cut(s) 210, 246
BseMI GCAATG 2 cut(s) 370, 452
BseMII CTCAG 1 cut(s) 1011
BseNI ACTGG 6 cut(s) 244, 287, 482, 553, 682, 917
BseRI GAGGAG 2 cut(s) 678, 785
BseSI GKGCMC 2 cut(s) 299, 1111
BseXI GCAGC 1 cut(s) 376
Bsh1236I CGCG 1 cut(s) 539
BshFI GGCC 4 cut(s) 286, 481, 1050, 1109
BsiHKCI CYCGRG 1 cut(s) 1163
BsiSI CCGG 2 cut(s) 493, 845
BslFI GGGAC 3 cut(s) 244, 641, 1013
BslI CCNNNNNNNGG 2 cut(s) 210, 246
BsmAI GTCTC 2 cut(s) 692, 866
BsmFI GGGAC 3 cut(s) 244, 641, 1013
BsnI GGCC 4 cut(s) 286, 481, 1050, 1109
BsoBI CYCGRG 1 cut(s) 1163
Bsp120I GGGCCC 1 cut(s) 1107
Bsp1286I GDGCHC 2 cut(s) 299, 1111
Bsp143I GATC 4 cut(s) 3, 49, 213, 310
BspACI CCGC 4 cut(s) 7, 204, 813, 848
BspANI GGCC 4 cut(s) 286, 481, 1050, 1109
BspCNI CTCAG 1 cut(s) 1010
BspFNI CGCG 1 cut(s) 539
BspLI GGNNCC 5 cut(s) 215, 232, 629, 1084, 1109
BspMAI CTGCAG 2 cut(s) 394, 971
BspPI GGATC 4 cut(s) 44, 208, 221, 305
BsrDI GCAATG 2 cut(s) 370, 452
BsrFI RCCGGY 1 cut(s) 492
BsrI ACTGG 6 cut(s) 244, 287, 482, 553, 682, 917
BssAI RCCGGY 1 cut(s) 492
BssECI CCNNGG 3 cut(s) 17, 843, 1051
BssMI GATC 4 cut(s) 3, 49, 213, 310
BssSI CACGAG 1 cut(s) 225
Bst2BI CACGAG 1 cut(s) 225
Bst2UI CCWGG 5 cut(s) 18, 108, 1019, 1035, 1053
Bst4CI ACNGT 4 cut(s) 88, 398, 530, 1045
Bst6I CTCTTC 1 cut(s) 878
BstDEI CTNAG 2 cut(s) 997, 1149
BstF5I GGATG 4 cut(s) 280, 633, 829, 952
BstFNI CGCG 1 cut(s) 539
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 2 cut(s) 142, 539
BstKTI GATC 4 cut(s) 6, 52, 216, 313
BstMAI GTCTC 2 cut(s) 692, 866
BstMBI GATC 4 cut(s) 3, 49, 213, 310
BstMWI GCNNNNNNNGC 1 cut(s) 845
BstNI CCWGG 5 cut(s) 18, 108, 1019, 1035, 1053
BstNSI RCATGY 1 cut(s) 648
BstPAI GACNNNNGTC 1 cut(s) 1101
BstSCI CCNGG 6 cut(s) 16, 106, 843, 1017, 1033, 1051
BstSFI CTRYAG 2 cut(s) 390, 967
BstSLI GKGCMC 2 cut(s) 299, 1111
BstUI CGCG 1 cut(s) 539
BstV1I GCAGC 1 cut(s) 376
BstX2I RGATCY 1 cut(s) 213
BstYI RGATCY 1 cut(s) 213
BsuRI GGCC 4 cut(s) 286, 481, 1050, 1109
BtsCI GGATG 4 cut(s) 280, 633, 829, 952
BtsIMutI CAGTG 7 cut(s) 251, 275, 280, 475, 535, 675, 924
CfoI GCGC 2 cut(s) 142, 539
Cfr10I RCCGGY 1 cut(s) 492
Cfr13I GGNCC 6 cut(s) 43, 231, 628, 1049, 1107, 1108
CseI GACGC 1 cut(s) 528
CsiI ACCWGGT 2 cut(s) 1017, 1033
Csp6I GTAC 9 cut(s) 301, 496, 681, 793, 964, 985, 1015, 1146, 1192
CviAII CATG 3 cut(s) 10, 645, 868
CviQI GTAC 9 cut(s) 301, 496, 681, 793, 964, 985, 1015, 1146, 1192
DdeI CTNAG 2 cut(s) 997, 1149
DpnI GATC 4 cut(s) 5, 51, 215, 312
DpnII GATC 4 cut(s) 3, 49, 213, 310
DraIII CACNNNGTG 1 cut(s) 1037
Eam1104I CTCTTC 1 cut(s) 878
EarI CTCTTC 1 cut(s) 878
EciI GGCGGA 1 cut(s) 863
Eco24I GRGCYC 1 cut(s) 1111
Eco47I GGWCC 3 cut(s) 43, 231, 628
Eco47III AGCGCT 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 1163
EcoRI GAATTC 1 cut(s) 830
EcoRII CCWGG 5 cut(s) 16, 106, 1017, 1033, 1051
EcoT38I GRGCYC 1 cut(s) 1111
FaeI CATG 3 cut(s) 13, 648, 871
FaqI GGGAC 3 cut(s) 244, 641, 1013
FatI CATG 3 cut(s) 9, 644, 867
Fnu4HI GCNGC 1 cut(s) 390
FokI GGATG 4 cut(s) 287, 620, 836, 939
FriOI GRGCYC 1 cut(s) 1111
Fsp4HI GCNGC 1 cut(s) 390
FspBI CTAG 2 cut(s) 810, 1061
GlaI GCGC 2 cut(s) 141, 538
GluI GCNGC 1 cut(s) 390
HaeII RGCGCY 1 cut(s) 143
HaeIII GGCC 4 cut(s) 286, 481, 1050, 1109
HapII CCGG 2 cut(s) 493, 845
HgaI GACGC 1 cut(s) 528
HhaI GCGC 2 cut(s) 142, 539
Hin1II CATG 3 cut(s) 13, 648, 871
Hin6I GCGC 2 cut(s) 140, 537
HinP1I GCGC 2 cut(s) 140, 537
HincII GTYRAC 1 cut(s) 747
HindII GTYRAC 1 cut(s) 747
HindIII AAGCTT 1 cut(s) 577
HpaII CCGG 2 cut(s) 493, 845
HphI GGTGA 5 cut(s) 136, 605, 714, 814, 827
Hpy166II GTNNAC 3 cut(s) 683, 747, 890
Hpy188I TCNGA 5 cut(s) 148, 213, 1000, 1087, 1137
Hpy188III TCNNGA 5 cut(s) 512, 697, 959, 1165, 1183
Hpy8I GTNNAC 3 cut(s) 683, 747, 890
Hpy99I CGWCG 1 cut(s) 88
HpyCH4III ACNGT 4 cut(s) 88, 398, 530, 1045
HpyCH4IV ACGT 4 cut(s) 293, 429, 785, 1114
HpyCH4V TGCA 6 cut(s) 368, 392, 781, 969, 981, 1040
HpyF10VI GCNNNNNNNGC 1 cut(s) 845
HpyF3I CTNAG 2 cut(s) 997, 1149
HpySE526I ACGT 4 cut(s) 293, 429, 785, 1114
Hsp92II CATG 3 cut(s) 13, 648, 871
HspAI GCGC 2 cut(s) 140, 537
Kzo9I GATC 4 cut(s) 3, 49, 213, 310
LmnI GCTCC 2 cut(s) 67, 1126
Lsp1109I GCAGC 1 cut(s) 376
MabI ACCWGGT 2 cut(s) 1017, 1033
MaeI CTAG 2 cut(s) 810, 1061
MaeII ACGT 4 cut(s) 293, 429, 785, 1114
MaeIII GTNAC 3 cut(s) 79, 425, 1094
MalI GATC 4 cut(s) 5, 51, 215, 312
MboI GATC 4 cut(s) 3, 49, 213, 310
MboII GAAGA 3 cut(s) 193, 383, 895
MflI RGATCY 1 cut(s) 213
MhlI GDGCHC 2 cut(s) 299, 1111
MluCI AATT 4 cut(s) 61, 668, 734, 830
MmeI TCCRAC 2 cut(s) 314, 907
MseI TTAA 1 cut(s) 56
MspI CCGG 2 cut(s) 493, 845
MspR9I CCNGG 6 cut(s) 18, 108, 845, 1019, 1035, 1053
MvaI CCWGG 5 cut(s) 18, 108, 1019, 1035, 1053
MvnI CGCG 1 cut(s) 539
MwoI GCNNNNNNNGC 1 cut(s) 845
NciI CCSGG 1 cut(s) 845
NdeII GATC 4 cut(s) 3, 49, 213, 310
NlaIII CATG 3 cut(s) 13, 648, 871
NlaIV GGNNCC 5 cut(s) 215, 232, 629, 1084, 1109
NmuCI GTSAC 1 cut(s) 1094
NspI RCATGY 1 cut(s) 648
PaeR7I CTCGAG 1 cut(s) 1163
PcsI WCGNNNNNNNCGW 1 cut(s) 1111
PkrI GCNGC 1 cut(s) 391
PshAI GACNNNNGTC 1 cut(s) 1101
Psp6I CCWGG 5 cut(s) 16, 106, 1017, 1033, 1051
PspGI CCWGG 5 cut(s) 16, 106, 1017, 1033, 1051
PspN4I GGNNCC 5 cut(s) 215, 232, 629, 1084, 1109
PspOMI GGGCCC 1 cut(s) 1107
PspPI GGNCC 6 cut(s) 43, 231, 628, 1049, 1107, 1108
PstI CTGCAG 2 cut(s) 394, 971
PsuI RGATCY 1 cut(s) 213
RsaI GTAC 9 cut(s) 302, 497, 682, 794, 965, 986, 1016, 1147, 1193
RsaNI GTAC 9 cut(s) 301, 496, 681, 793, 964, 985, 1015, 1146, 1192
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 1 cut(s) 390
Sau3AI GATC 4 cut(s) 3, 49, 213, 310
Sau96I GGNCC 6 cut(s) 43, 231, 628, 1049, 1107, 1108
ScaI AGTACT 1 cut(s) 965
ScrFI CCNGG 6 cut(s) 18, 108, 845, 1019, 1035, 1053
SduI GDGCHC 2 cut(s) 299, 1111
SexAI ACCWGGT 2 cut(s) 1017, 1033
SfcI CTRYAG 2 cut(s) 390, 967
Sfr274I CTCGAG 1 cut(s) 1163
SinI GGWCC 3 cut(s) 43, 231, 628
SlaI CTCGAG 1 cut(s) 1163
SmlI CTYRAG 5 cut(s) 354, 405, 959, 1163, 1181
SmoI CTYRAG 5 cut(s) 354, 405, 959, 1163, 1181
Sse9I AATT 4 cut(s) 61, 668, 734, 830
SsiI CCGC 4 cut(s) 7, 204, 813, 848
SspMI CTAG 2 cut(s) 810, 1061
StyD4I CCNGG 6 cut(s) 16, 106, 843, 1017, 1033, 1051
TaaI ACNGT 4 cut(s) 88, 398, 530, 1045
TaiI ACGT 4 cut(s) 296, 432, 788, 1117
TaqI TCGA 3 cut(s) 413, 696, 1164
TasI AATT 4 cut(s) 61, 668, 734, 830
TatI WGTACW 2 cut(s) 963, 984
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TscAI CASTG 7 cut(s) 251, 275, 287, 482, 535, 682, 924
TseFI GTSAC 1 cut(s) 1094
TseI GCWGC 1 cut(s) 389
Tsp45I GTSAC 1 cut(s) 1094
TspDTI ATGAA 4 cut(s) 26, 200, 209, 549
TspGWI ACGGA 5 cut(s) 35, 42, 159, 764, 1002
TspRI CASTG 7 cut(s) 251, 275, 287, 482, 535, 682, 924
VpaK11BI GGWCC 3 cut(s) 43, 231, 628
XapI RAATTY 2 cut(s) 734, 830
XceI RCATGY 1 cut(s) 648
XcmI CCANNNNNNNNNTGG 2 cut(s) 251, 1185
XhoI CTCGAG 1 cut(s) 1163
XspI CTAG 2 cut(s) 810, 1061
ZrmI AGTACT 1 cut(s) 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.