Rorug04G0169600

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0015414
Physical Location & Seq
Reverse (-)
29866184 .. 29874882
8699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0169600.1

Sequence Viewer

Length: 1113 bp
ATGAAAGCTGCTCGAAAACCCCAAGCACAGCGCAACCGACCCAAACACCATCACATCATCACATCATCACCCGAACCCACATCCTCCCCCAATTTCTTAGCCTCCAAGAGCCTCTTCCCCATTCCCAGAAGCCGACCCTTTCTTCTCTTATCTCCCCCTCACCCGCAAACCATAAAGGAAGAAGCTCAAAGTTTTGGTTCTAAGCAACATCACCACGAAGATCGGGAAGCAGAGACACTCTTCATCCTCTCTTTACTACAGAAGAATTTTGGTGAGGAAAAGCTAGCAACAGAACTTACGATTCGGGTTTTGGTTCGAGTTCCTGAAGATGTAGAGTTGAGATTCATTGTTACCGTGGAAGCTGTTGCATGGAAATTTCTTAACAGATTGCAGCGTACAGATAGAACCGAATATGTTGCAAGATGCTCTGTTATACTTGAGATTATGGCTGTCATGGAAAAGCATTCCCATAGCCAAAAAAATTCAGAGATGAATTTTGAAAGAGCTGTCACGTTGTCTAGAACAAGGCGTGTCCCTGTGAAGGGTTATTATTACATTATAAGCACTAAACAATCCCTTAACTTCATGAAGTTCCGCACCAAGGCAAGGTATCCATTTTTATTTAGCACATATGTTCCAACAGATCATCATCTTGTGCCTGGCAGGCTTTTCTCTTATTATAATGGCAAGATGGAGGGAGGACTTAGTCCCAGGCAAGAAATTGGTTCCCATTCAAAATGGAAGCAATATCCCCCAAATTTGTTTCACCTTCAAATTTTGTGGGCAACCAACCAAATTGGTGTTGCCCTTGTTTGTCTTAGTGGAGAGCCAAAATGGTATGGTGTACTTTTTGTGTCAAAAATGTTGAAGGAACCAGCGATCGATGCTGGTAAACTATGGTGTTCCGGATGTGGCAAGGTTTTAGAGGATCACATTTTCTCTGAGGAACCCACTTTCGTTAAGAATGCGGCGGGCCAGAGCCAATTGGCTGGAAGATACGTACATTCTAGGGAAAGCGAAATCTCAGCTTCTCGTGAAAGGATACTCGAAAATGGTCTGTTTTCTTGCGTTTTTGCTCTTTCAATGTTTGGCTTGTTCAAGCTTTTCTTGTAG

Protein Analysis

370

Amino Acids

42.48

Weight (kDa)

9.63

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18110
fragaria_vesca FvH4_4g17300 FvH4_4g17300
malus_domestica MD07G1304500.v1.1
prunus_persica Prupe.6G174500_v2.0.a1
pyrus_communis pycom07g27730
rosa_chinensis RchiOBHm_Chr4g0421271
rosa_laevigata RLG00000007705
rosa_multiflora Rmu_sc0009290.1_g000019
rosa_roxburghii Rroxscaffold_5G00363500
rosa_rugosa Rorug04G0169600
rosa_samantha Rh4AG230100 Rh4BG232900 Rh4CG244500 Rh4DG228700
rosa_wichuraiana Rw4G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 560, 681
AccIII TCCGGA 1 cut(s) 905
AciI CCGC 4 cut(s) 164, 595, 968, 971
AclWI GGATC 1 cut(s) 936
AcsI RAATTY 6 cut(s) 265, 374, 481, 493, 757, 774
AcuI CTGAAG 1 cut(s) 345
AfaI GTAC 3 cut(s) 397, 846, 1002
AfiI CCNNNNNNNGG 4 cut(s) 541, 542, 601, 606
AgsI TTSAA 6 cut(s) 500, 735, 773, 868, 1083, 1099
AjnI CCWGG 2 cut(s) 658, 710
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AluBI AGCT 7 cut(s) 8, 185, 283, 362, 506, 1028, 1102
AluI AGCT 7 cut(s) 8, 185, 283, 362, 506, 1028, 1102
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 1 cut(s) 936
Aor13HI TCCGGA 1 cut(s) 905
AoxI GGCC 1 cut(s) 973
ApeKI GCWGC 2 cut(s) 8, 391
ApoI RAATTY 6 cut(s) 265, 374, 481, 493, 757, 774
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 973
AsuHPI GGTGA 5 cut(s) 60, 152, 203, 284, 758
AsuNHI GCTAGC 1 cut(s) 283
BauI CACGAG 1 cut(s) 1032
BbvI GCAGC 1 cut(s) 403
BccI CCATC 2 cut(s) 57, 685
BcgI CGANNNNNNTGC 2 cut(s) 398, 432
BciT130I CCWGG 2 cut(s) 660, 712
BciVI GTATCC 2 cut(s) 621, 1035
BcoDI GTCTC 1 cut(s) 227
BfaI CTAG 3 cut(s) 284, 519, 1008
BfmI CTRYAG 1 cut(s) 257
BfuI GTATCC 2 cut(s) 621, 1035
BglI GCCNNNNNGGC 1 cut(s) 664
BisI GCNGC 3 cut(s) 9, 392, 969
BlsI GCNGC 3 cut(s) 10, 393, 970
Bme1390I CCNGG 2 cut(s) 660, 712
BmgT120I GGNCC 1 cut(s) 973
BmiI GGNNCC 3 cut(s) 727, 873, 948
BmrFI CCNGG 2 cut(s) 660, 712
BmsI GCATC 2 cut(s) 413, 874
BmtI GCTAGC 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 458
Bsa29I ATCGAT 1 cut(s) 882
BsaAI YACGTR 1 cut(s) 1000
BsaBI GATNNNNATC 1 cut(s) 648
BsaJI CCNNGG 3 cut(s) 354, 600, 710
BsaWI WCCGGW 1 cut(s) 905
Bsc4I CCNNNNNNNGG 4 cut(s) 541, 542, 601, 606
Bse8I GATNNNNATC 1 cut(s) 648
BseAI TCCGGA 1 cut(s) 905
BseBI CCWGG 2 cut(s) 660, 712
BseCI ATCGAT 1 cut(s) 882
BseDI CCNNGG 3 cut(s) 354, 600, 710
BseGI GGATG 3 cut(s) 80, 243, 914
BseJI GATNNNNATC 1 cut(s) 648
BseLI CCNNNNNNNGG 4 cut(s) 541, 542, 601, 606
BseMII CTCAG 2 cut(s) 933, 1038
BseXI GCAGC 1 cut(s) 403
Bsh1285I CGRYCG 1 cut(s) 882
BshFI GGCC 1 cut(s) 975
BshVI ATCGAT 1 cut(s) 882
BsiEI CGRYCG 1 cut(s) 882
BsiSI CCGG 1 cut(s) 906
BslFI GGGAC 2 cut(s) 518, 693
BslI CCNNNNNNNGG 4 cut(s) 541, 542, 601, 606
BsmAI GTCTC 1 cut(s) 227
BsmFI GGGAC 2 cut(s) 518, 693
BsmI GAATGC 2 cut(s) 463, 970
BsnI GGCC 1 cut(s) 975
Bsp13I TCCGGA 1 cut(s) 905
Bsp143I GATC 4 cut(s) 220, 643, 879, 928
BspACI CCGC 4 cut(s) 164, 595, 968, 971
BspANI GGCC 1 cut(s) 975
BspCNI CTCAG 2 cut(s) 934, 1037
BspDI ATCGAT 1 cut(s) 882
BspEI TCCGGA 1 cut(s) 905
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 3 cut(s) 727, 873, 948
BspOI GCTAGC 1 cut(s) 287
BspPI GGATC 1 cut(s) 936
BssECI CCNNGG 3 cut(s) 354, 600, 710
BssMI GATC 4 cut(s) 220, 643, 879, 928
BssSI CACGAG 1 cut(s) 1032
BssT1I CCWWGG 1 cut(s) 600
Bst2BI CACGAG 1 cut(s) 1032
Bst2UI CCWGG 2 cut(s) 660, 712
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 2 cut(s) 119, 245
BstBAI YACGTR 1 cut(s) 1000
BstC8I GCNNGC 3 cut(s) 285, 665, 973
BstDEI CTNAG 6 cut(s) 97, 201, 704, 818, 942, 1024
BstDSI CCRYGG 1 cut(s) 354
BstF5I GGATG 3 cut(s) 80, 243, 914
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 4 cut(s) 223, 646, 882, 931
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 4 cut(s) 220, 643, 879, 928
BstMCI CGRYCG 1 cut(s) 882
BstMWI GCNNNNNNNGC 2 cut(s) 664, 884
BstNI CCWGG 2 cut(s) 660, 712
BstSCI CCNGG 2 cut(s) 658, 710
BstSFI CTRYAG 1 cut(s) 257
BstSNI TACGTA 1 cut(s) 1000
BstV1I GCAGC 1 cut(s) 403
BstXI CCANNNNNNTGG 1 cut(s) 989
Bsu15I ATCGAT 1 cut(s) 882
BsuI GTATCC 2 cut(s) 621, 1035
BsuRI GGCC 1 cut(s) 975
BsuTUI ATCGAT 1 cut(s) 882
BtgI CCRYGG 1 cut(s) 354
BtsCI GGATG 3 cut(s) 80, 243, 914
Cac8I GCNNGC 3 cut(s) 285, 665, 973
CciI TCATGA 1 cut(s) 585
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 1 cut(s) 973
ClaI ATCGAT 1 cut(s) 882
Csp6I GTAC 3 cut(s) 396, 845, 1001
CspCI CAANNNNNGTGG 2 cut(s) 761, 796
CviAII CATG 3 cut(s) 369, 454, 586
CviQI GTAC 3 cut(s) 396, 845, 1001
DdeI CTNAG 6 cut(s) 97, 201, 704, 818, 942, 1024
DpnI GATC 4 cut(s) 222, 645, 881, 930
DpnII GATC 4 cut(s) 220, 643, 879, 928
Eam1104I CTCTTC 2 cut(s) 119, 245
EarI CTCTTC 2 cut(s) 119, 245
Eco105I TACGTA 1 cut(s) 1000
Eco130I CCWWGG 1 cut(s) 600
Eco57I CTGAAG 1 cut(s) 345
EcoRII CCWGG 2 cut(s) 658, 710
EcoT14I CCWWGG 1 cut(s) 600
ErhI CCWWGG 1 cut(s) 600
FaeI CATG 3 cut(s) 372, 457, 589
FalI AAGNNNNNCTT 3 cut(s) 98, 130, 1091
FaqI GGGAC 2 cut(s) 518, 693
FatI CATG 3 cut(s) 368, 453, 585
FauI CCCGC 2 cut(s) 171, 964
FauNDI CATATG 1 cut(s) 631
Fnu4HI GCNGC 3 cut(s) 9, 392, 969
FokI GGATG 3 cut(s) 67, 230, 921
Fsp4HI GCNGC 3 cut(s) 9, 392, 969
FspBI CTAG 3 cut(s) 284, 519, 1008
GlaI GCGC 1 cut(s) 32
GluI GCNGC 3 cut(s) 9, 392, 969
HaeIII GGCC 1 cut(s) 975
HapII CCGG 1 cut(s) 906
HhaI GCGC 1 cut(s) 33
Hin1II CATG 3 cut(s) 372, 457, 589
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HindIII AAGCTT 1 cut(s) 1100
HinfI GANTC 2 cut(s) 301, 342
HpaII CCGG 1 cut(s) 906
HphI GGTGA 5 cut(s) 60, 152, 203, 284, 758
Hpy166II GTNNAC 2 cut(s) 845, 893
Hpy188I TCNGA 2 cut(s) 487, 943
Hpy188III TCNNGA 6 cut(s) 224, 323, 519, 586, 906, 1034
Hpy8I GTNNAC 2 cut(s) 845, 893
HpyAV CCTTC 3 cut(s) 535, 779, 862
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 2 cut(s) 512, 999
HpyCH4V TGCA 3 cut(s) 368, 391, 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 664, 884
HpyF3I CTNAG 6 cut(s) 97, 201, 704, 818, 942, 1024
HpySE526I ACGT 2 cut(s) 512, 999
Hsp92II CATG 3 cut(s) 372, 457, 589
HspAI GCGC 1 cut(s) 31
Kpn2I TCCGGA 1 cut(s) 905
Kzo9I GATC 4 cut(s) 220, 643, 879, 928
Lsp1109I GCAGC 1 cut(s) 403
LweI GCATC 2 cut(s) 413, 874
MaeI CTAG 3 cut(s) 284, 519, 1008
MaeII ACGT 2 cut(s) 512, 999
MaeIII GTNAC 2 cut(s) 349, 508
MalI GATC 4 cut(s) 222, 645, 881, 930
MboI GATC 4 cut(s) 220, 643, 879, 928
MboII GAAGA 8 cut(s) 106, 134, 191, 230, 232, 274, 338, 1005
MfeI CAATTG 1 cut(s) 983
MmeI TCCRAC 1 cut(s) 662
MroI TCCGGA 1 cut(s) 905
MseI TTAA 3 cut(s) 381, 579, 960
MspI CCGG 1 cut(s) 906
MspR9I CCNGG 2 cut(s) 660, 712
MunI CAATTG 1 cut(s) 983
Mva1269I GAATGC 2 cut(s) 463, 970
MvaI CCWGG 2 cut(s) 660, 712
MwoI GCNNNNNNNGC 2 cut(s) 664, 884
NdeI CATATG 1 cut(s) 631
NdeII GATC 4 cut(s) 220, 643, 879, 928
NheI GCTAGC 1 cut(s) 283
NlaIII CATG 3 cut(s) 372, 457, 589
NlaIV GGNNCC 3 cut(s) 727, 873, 948
NmuCI GTSAC 1 cut(s) 508
PagI TCATGA 1 cut(s) 585
PctI GAATGC 2 cut(s) 463, 970
PfeI GAWTC 2 cut(s) 301, 342
PflFI GACNNNGTC 1 cut(s) 705
PkrI GCNGC 3 cut(s) 10, 393, 970
Ple19I CGATCG 1 cut(s) 882
Ppu21I YACGTR 1 cut(s) 1000
PsiI TTATAA 2 cut(s) 560, 681
Psp6I CCWGG 2 cut(s) 658, 710
PspGI CCWGG 2 cut(s) 658, 710
PspN4I GGNNCC 3 cut(s) 727, 873, 948
PspPI GGNCC 1 cut(s) 973
PsyI GACNNNGTC 1 cut(s) 705
PvuI CGATCG 1 cut(s) 882
RsaI GTAC 3 cut(s) 397, 846, 1002
RsaNI GTAC 3 cut(s) 396, 845, 1001
SaqAI TTAA 3 cut(s) 381, 579, 960
SatI GCNGC 3 cut(s) 9, 392, 969
Sau3AI GATC 4 cut(s) 220, 643, 879, 928
Sau96I GGNCC 1 cut(s) 973
ScrFI CCNGG 2 cut(s) 660, 712
SfaNI GCATC 2 cut(s) 413, 874
SfcI CTRYAG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SnaBI TACGTA 1 cut(s) 1000
SsiI CCGC 4 cut(s) 164, 595, 968, 971
SspMI CTAG 3 cut(s) 284, 519, 1008
StyD4I CCNGG 2 cut(s) 658, 710
StyI CCWWGG 1 cut(s) 600
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 2 cut(s) 515, 1002
TaqI TCGA 4 cut(s) 13, 316, 882, 1047
TatI WGTACW 1 cut(s) 844
TauI GCSGC 1 cut(s) 971
TfiI GAWTC 2 cut(s) 301, 342
Tru1I TTAA 3 cut(s) 381, 579, 960
Tru9I TTAA 3 cut(s) 381, 579, 960
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 2 cut(s) 8, 391
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 6 cut(s) 17, 232, 334, 506, 574, 602
Tth111I GACNNNGTC 1 cut(s) 705
XapI RAATTY 6 cut(s) 265, 374, 481, 493, 757, 774
XbaI TCTAGA 1 cut(s) 518
XspI CTAG 3 cut(s) 284, 519, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.