Rh4AG230300

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016400
Physical Location & Seq
Forward (+)
53309491 .. 53311809
2319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG230300.1

Sequence Viewer

Length: 1251 bp
ATGATCCGCATGAAACCTGGGAAGCTAAAACCAAAAGTGCTGGTCCGTGATCCGTTAAAGAATTGGGAGGAAAAATGGGTAACGACTGTGGAGAACCCGGAAAAACCAGGACAAATCCTATGGGTGAAATACATGGAAGGGCTGTCGGAGAGCGATTTAGATGTACCTCCGTTGAGTGAGAAGTTGAAAGAAACGAGAAAAGCCGCTGCCCGGTTCCTTTCAACTCGTGGGACCAAAACCGACCGGTGGATGTGTGGGGTTTGTTATCAGTGGGATGATCACTGGCCTCCAACGTGCCCATACACGGATGATCCTACACGACCACGACCCCACTCCAAAACGGCAAAACTCATACAGTCTAAGATGCAGGCTCAAGGTAGAAATGCAGAAGAACCAAAGGATAATGCTGCAGACTGTTTGGCTCAAGCCTCGAAAAAAAGTGGTAACGTGTATAGCCATTGGCAATGTAGGATTTGTGGTAAGATAGATGACCACTGGCCTCCAATGTGCCCGTACAAGTGTATCTATCCCGAATTTGCTGAATACAGTGATAGCGCGTCTGCTAGTGGCATGAGTACAGTCCAAATGCCTGAAAGCTTGACCAAAGGAGGTGATGTTATGTTTTGTGGGATTTGTGGTGCGAGGGACCACATCCCTAAAATATGCTACTACAAGCTTTTTGTCAAATTATTCACTGGTACACCTCCACCTGTCAAGACTATTGATGTTCGTCCAAGCTCACCAGAAAGGAAATTCACTTGGTCGACGGACTTCCTTGTTGTGCCAGAGCAGTTTTTACGCACGTCAGTGTACCAATGGGTGAAGCCCAGCGGTGATGCGGATGAGGAATTCTTTGCTTCCCCGGCGGAGATGGTTGAATATATCATGCAGACAAACAGGAAGAATAAACCAACAGGTAGTATGGATGAAAAACCAGTGGTTATTGGAGGTGCGTTTACTTCTCCACGACATCCTCTTGAGTACTGTAGGATTTGTTGCAAGTACATTGACCACTCAGATATAACTTGTCCGTACCTGGAATATATCCCGCCTGGTGCAACTGTTGGCCCTGGCTGTCTAGTAGTTTGTAGTATGTGTGGTTCCGAGAATTGTGAAGAAGGTCGGGCCCAACGTGTTGCTACCGTGTCTTGTCTGAAGTGTGGTACTAAGTTTGACCACCTCGAGAGAGATTGCCCACTCAAGAACAGCTGCCCACCTAACTTCAGAGATTGCCCACCTCTGAAGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

416

Amino Acids

47.0

Weight (kDa)

8.19

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016400)

Species Orthologous Gene IDs
malus_domestica MD10G1001800.v1.1
pyrus_communis pycom10g00170
rosa_laevigata RLG00000007703 RLG00000007704
rosa_multiflora Rmu_sc0001162.1_g000001
rosa_rugosa Rorug04G0169600 Rorug04G0175900
rosa_samantha Rh4AG230300 Rh4AG234900 Rh4BG233200 Rh4CG249800 Rh4DG229000 Rh4DG233800
rosa_wichuraiana Rw4G019940 Rw4G020350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 764
AccII CGCG 1 cut(s) 557
AciI CCGC 6 cut(s) 7, 204, 831, 839, 866, 1049
AclWI GGATC 2 cut(s) 44, 305
AcsI RAATTY 3 cut(s) 533, 752, 848
AcuI CTGAAG 2 cut(s) 1175, 1207
AfaI GTAC 9 cut(s) 165, 515, 577, 700, 812, 983, 1004, 1034, 1165
AfiI CCNNNNNNNGG 3 cut(s) 210, 246, 304
AflIII ACRYGT 2 cut(s) 447, 1132
AgeI ACCGGT 1 cut(s) 243
AgsI TTSAA 3 cut(s) 187, 222, 878
AjiI CACGTC 1 cut(s) 804
AjnI CCWGG 5 cut(s) 16, 106, 1035, 1051, 1069
AleI CACNNNNGTG 1 cut(s) 806
AluBI AGCT 5 cut(s) 25, 597, 676, 738, 1209
AluI AGCT 5 cut(s) 25, 597, 676, 738, 1209
AlwI GGATC 2 cut(s) 44, 305
Ama87I CYCGRG 1 cut(s) 1181
AoxI GGCC 4 cut(s) 284, 497, 1066, 1125
ApaI GGGCCC 1 cut(s) 1129
ApeKI GCWGC 3 cut(s) 206, 407, 1209
ApoI RAATTY 3 cut(s) 533, 752, 848
AsiGI ACCGGT 1 cut(s) 243
AspLEI GCGC 1 cut(s) 557
AspS9I GGNCC 6 cut(s) 43, 231, 646, 1067, 1125, 1126
AsuC2I CCSGG 3 cut(s) 98, 211, 863
AsuHPI GGTGA 5 cut(s) 136, 623, 732, 832, 845
AvaI CYCGRG 1 cut(s) 1181
AvaII GGWCC 3 cut(s) 43, 231, 646
BaeGI GKGCMC 3 cut(s) 299, 512, 1129
BaeI ACNNNNGTAYC 2 cut(s) 505, 538
BanII GRGCYC 1 cut(s) 1129
BauI CACGAG 1 cut(s) 225
BbvI GCAGC 3 cut(s) 193, 394, 1196
BccI CCATC 1 cut(s) 865
BceAI ACGGC 1 cut(s) 357
BciT130I CCWGG 5 cut(s) 18, 108, 1037, 1053, 1071
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 3 cut(s) 98, 211, 863
BfaI CTAG 2 cut(s) 564, 1079
BfmI CTRYAG 2 cut(s) 408, 985
BisI GCNGC 4 cut(s) 204, 207, 408, 1210
BlsI GCNGC 4 cut(s) 205, 208, 409, 1211
BmcAI AGTACT 1 cut(s) 983
Bme1390I CCNGG 8 cut(s) 18, 98, 108, 211, 863, 1037, 1053, 1071
Bme18I GGWCC 3 cut(s) 43, 231, 646
BmeT110I CYCGRG 1 cut(s) 1181
BmgBI CACGTC 1 cut(s) 804
BmgT120I GGNCC 6 cut(s) 43, 231, 646, 1067, 1125, 1126
BmiI GGNNCC 5 cut(s) 215, 232, 647, 1102, 1127
BmrFI CCNGG 8 cut(s) 18, 98, 108, 211, 863, 1037, 1053, 1071
BmsI GCATC 2 cut(s) 354, 826
BpuEI CTTGAG 4 cut(s) 357, 408, 998, 1184
BpuMI CCSGG 3 cut(s) 98, 211, 863
BsaJI CCNNGG 3 cut(s) 17, 861, 1069
BsaWI WCCGGW 1 cut(s) 243
BsaXI ACNNNNNCTCC 2 cut(s) 600, 630
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 246, 304
Bse118I RCCGGY 1 cut(s) 243
Bse1I ACTGG 4 cut(s) 287, 500, 700, 935
Bse3DI GCAATG 1 cut(s) 470
BseBI CCWGG 5 cut(s) 18, 108, 1037, 1053, 1071
BseDI CCNNGG 3 cut(s) 17, 861, 1069
BseGI GGATG 7 cut(s) 255, 280, 313, 651, 847, 931, 970
BseLI CCNNNNNNNGG 3 cut(s) 210, 246, 304
BseMI GCAATG 1 cut(s) 470
BseMII CTCAG 1 cut(s) 1029
BseNI ACTGG 4 cut(s) 287, 500, 700, 935
BseSI GKGCMC 3 cut(s) 299, 512, 1129
BseXI GCAGC 3 cut(s) 193, 394, 1196
BseYI CCCAGC 1 cut(s) 827
Bsh1236I CGCG 1 cut(s) 557
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 4 cut(s) 286, 499, 1068, 1127
BshTI ACCGGT 1 cut(s) 243
BsiEI CGRYCG 1 cut(s) 244
BsiHKCI CYCGRG 1 cut(s) 1181
BsiSI CCGG 4 cut(s) 98, 211, 244, 863
BslFI GGGAC 2 cut(s) 244, 659
BslI CCNNNNNNNGG 3 cut(s) 210, 246, 304
BsmFI GGGAC 2 cut(s) 244, 659
BsnI GGCC 4 cut(s) 286, 499, 1068, 1127
BsoBI CYCGRG 1 cut(s) 1181
Bsp120I GGGCCC 1 cut(s) 1125
Bsp1286I GDGCHC 3 cut(s) 299, 512, 1129
Bsp143I GATC 4 cut(s) 3, 49, 277, 310
BspACI CCGC 6 cut(s) 7, 204, 831, 839, 866, 1049
BspANI GGCC 4 cut(s) 286, 499, 1068, 1127
BspCNI CTCAG 1 cut(s) 1028
BspFNI CGCG 1 cut(s) 557
BspLI GGNNCC 5 cut(s) 215, 232, 647, 1102, 1127
BspMAI CTGCAG 1 cut(s) 412
BspPI GGATC 2 cut(s) 44, 305
BsrDI GCAATG 1 cut(s) 470
BsrFI RCCGGY 1 cut(s) 243
BsrI ACTGG 4 cut(s) 287, 500, 700, 935
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 3 cut(s) 17, 861, 1069
BssMI GATC 4 cut(s) 3, 49, 277, 310
BssSI CACGAG 1 cut(s) 225
Bst2BI CACGAG 1 cut(s) 225
Bst2UI CCWGG 5 cut(s) 18, 108, 1037, 1053, 1071
Bst4CI ACNGT 8 cut(s) 88, 357, 416, 548, 580, 986, 1063, 1144
BstC8I GCNNGC 1 cut(s) 369
BstDEI CTNAG 3 cut(s) 360, 1015, 1167
BstF5I GGATG 7 cut(s) 255, 280, 313, 651, 847, 931, 970
BstFNI CGCG 1 cut(s) 557
BstHHI GCGC 1 cut(s) 557
BstKTI GATC 4 cut(s) 6, 52, 280, 313
BstMBI GATC 4 cut(s) 3, 49, 277, 310
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 1 cut(s) 863
BstNI CCWGG 5 cut(s) 18, 108, 1037, 1053, 1071
BstSCI CCNGG 8 cut(s) 16, 96, 106, 209, 861, 1035, 1051, 1069
BstSFI CTRYAG 2 cut(s) 408, 985
BstSLI GKGCMC 3 cut(s) 299, 512, 1129
BstUI CGCG 1 cut(s) 557
BstV1I GCAGC 3 cut(s) 193, 394, 1196
BsuRI GGCC 4 cut(s) 286, 499, 1068, 1127
BtrI CACGTC 1 cut(s) 804
BtsCI GGATG 7 cut(s) 255, 280, 313, 651, 847, 931, 970
BtsIMutI CAGTG 7 cut(s) 275, 280, 493, 553, 693, 813, 942
Cac8I GCNNGC 1 cut(s) 369
CfoI GCGC 1 cut(s) 557
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 6 cut(s) 43, 231, 646, 1067, 1125, 1126
CseI GACGC 1 cut(s) 546
Csp6I GTAC 9 cut(s) 164, 514, 576, 699, 811, 982, 1003, 1033, 1164
CspAI ACCGGT 1 cut(s) 243
CviAII CATG 4 cut(s) 10, 133, 571, 886
CviQI GTAC 9 cut(s) 164, 514, 576, 699, 811, 982, 1003, 1033, 1164
DdeI CTNAG 3 cut(s) 360, 1015, 1167
DpnI GATC 4 cut(s) 5, 51, 279, 312
DpnII GATC 4 cut(s) 3, 49, 277, 310
EciI GGCGGA 1 cut(s) 881
Eco24I GRGCYC 1 cut(s) 1129
Eco47I GGWCC 3 cut(s) 43, 231, 646
Eco57I CTGAAG 2 cut(s) 1175, 1207
Eco88I CYCGRG 1 cut(s) 1181
EcoRI GAATTC 1 cut(s) 848
EcoRII CCWGG 5 cut(s) 16, 106, 1035, 1051, 1069
EcoT38I GRGCYC 1 cut(s) 1129
FaeI CATG 4 cut(s) 13, 136, 574, 889
FaqI GGGAC 2 cut(s) 244, 659
FatI CATG 4 cut(s) 9, 132, 570, 885
FauI CCCGC 1 cut(s) 1056
FbaI TGATCA 1 cut(s) 277
FblI GTMKAC 1 cut(s) 764
Fnu4HI GCNGC 4 cut(s) 204, 207, 408, 1210
FokI GGATG 7 cut(s) 262, 287, 320, 638, 854, 938, 957
FriOI GRGCYC 1 cut(s) 1129
Fsp4HI GCNGC 4 cut(s) 204, 207, 408, 1210
FspBI CTAG 2 cut(s) 564, 1079
GlaI GCGC 1 cut(s) 556
GluI GCNGC 4 cut(s) 204, 207, 408, 1210
GsaI CCCAGC 1 cut(s) 831
HaeIII GGCC 4 cut(s) 286, 499, 1068, 1127
HapII CCGG 4 cut(s) 98, 211, 244, 863
HgaI GACGC 1 cut(s) 546
HhaI GCGC 1 cut(s) 557
Hin1II CATG 4 cut(s) 13, 136, 574, 889
Hin6I GCGC 1 cut(s) 555
HinP1I GCGC 1 cut(s) 555
HincII GTYRAC 1 cut(s) 765
HindII GTYRAC 1 cut(s) 765
HindIII AAGCTT 2 cut(s) 595, 674
HpaII CCGG 4 cut(s) 98, 211, 244, 863
HphI GGTGA 5 cut(s) 136, 623, 732, 832, 845
Hpy166II GTNNAC 4 cut(s) 701, 765, 811, 957
Hpy188I TCNGA 6 cut(s) 148, 1018, 1105, 1155, 1226, 1242
Hpy188III TCNNGA 5 cut(s) 530, 715, 977, 1183, 1201
Hpy8I GTNNAC 4 cut(s) 701, 765, 811, 957
Hpy99I CGWCG 1 cut(s) 769
HpyAV CCTTC 2 cut(s) 131, 1112
HpyCH4III ACNGT 8 cut(s) 88, 357, 416, 548, 580, 986, 1063, 1144
HpyCH4IV ACGT 4 cut(s) 293, 447, 803, 1132
HpyCH4V TGCA 6 cut(s) 367, 386, 410, 889, 999, 1058
HpyF10VI GCNNNNNNNGC 1 cut(s) 863
HpyF3I CTNAG 3 cut(s) 360, 1015, 1167
HpySE526I ACGT 4 cut(s) 293, 447, 803, 1132
Hsp92II CATG 4 cut(s) 13, 136, 574, 889
HspAI GCGC 1 cut(s) 555
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 4 cut(s) 3, 49, 277, 310
Lsp1109I GCAGC 3 cut(s) 193, 394, 1196
LweI GCATC 2 cut(s) 354, 826
MaeI CTAG 2 cut(s) 564, 1079
MaeII ACGT 4 cut(s) 293, 447, 803, 1132
MaeIII GTNAC 2 cut(s) 79, 443
MalI GATC 4 cut(s) 5, 51, 279, 312
MboI GATC 4 cut(s) 3, 49, 277, 310
MboII GAAGA 3 cut(s) 401, 913, 1127
MhlI GDGCHC 3 cut(s) 299, 512, 1129
MluCI AATT 6 cut(s) 61, 533, 686, 752, 848, 1108
MmeI TCCRAC 2 cut(s) 126, 314
MseI TTAA 1 cut(s) 56
MslI CAYNNNNRTG 1 cut(s) 806
MspA1I CMGCKG 3 cut(s) 206, 831, 1209
MspI CCGG 4 cut(s) 98, 211, 244, 863
MspR9I CCNGG 8 cut(s) 18, 98, 108, 211, 863, 1037, 1053, 1071
MvaI CCWGG 5 cut(s) 18, 108, 1037, 1053, 1071
MvnI CGCG 1 cut(s) 557
MwoI GCNNNNNNNGC 1 cut(s) 863
NciI CCSGG 3 cut(s) 98, 211, 863
NdeII GATC 4 cut(s) 3, 49, 277, 310
NlaIII CATG 4 cut(s) 13, 136, 574, 889
NlaIV GGNNCC 5 cut(s) 215, 232, 647, 1102, 1127
OliI CACNNNNGTG 1 cut(s) 806
PaeR7I CTCGAG 1 cut(s) 1181
PcsI WCGNNNNNNNCGW 1 cut(s) 1129
PinAI ACCGGT 1 cut(s) 243
PkrI GCNGC 4 cut(s) 205, 208, 409, 1211
Psp6I CCWGG 5 cut(s) 16, 106, 1035, 1051, 1069
PspFI CCCAGC 1 cut(s) 827
PspGI CCWGG 5 cut(s) 16, 106, 1035, 1051, 1069
PspN4I GGNNCC 5 cut(s) 215, 232, 647, 1102, 1127
PspOMI GGGCCC 1 cut(s) 1125
PspPI GGNCC 6 cut(s) 43, 231, 646, 1067, 1125, 1126
PsrI GAACNNNNNNTAC 2 cut(s) 1084, 1116
PstI CTGCAG 1 cut(s) 412
PvuII CAGCTG 1 cut(s) 1209
RsaI GTAC 9 cut(s) 165, 515, 577, 700, 812, 983, 1004, 1034, 1165
RsaNI GTAC 9 cut(s) 164, 514, 576, 699, 811, 982, 1003, 1033, 1164
RseI CAYNNNNRTG 1 cut(s) 806
SalI GTCGAC 1 cut(s) 763
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 4 cut(s) 204, 207, 408, 1210
Sau3AI GATC 4 cut(s) 3, 49, 277, 310
Sau96I GGNCC 6 cut(s) 43, 231, 646, 1067, 1125, 1126
ScaI AGTACT 1 cut(s) 983
ScrFI CCNGG 8 cut(s) 18, 98, 108, 211, 863, 1037, 1053, 1071
SduI GDGCHC 3 cut(s) 299, 512, 1129
SfaNI GCATC 2 cut(s) 354, 826
SfcI CTRYAG 2 cut(s) 408, 985
Sfr274I CTCGAG 1 cut(s) 1181
SinI GGWCC 3 cut(s) 43, 231, 646
SlaI CTCGAG 1 cut(s) 1181
SmiMI CAYNNNNRTG 1 cut(s) 806
SmlI CTYRAG 5 cut(s) 372, 423, 977, 1181, 1199
SmoI CTYRAG 5 cut(s) 372, 423, 977, 1181, 1199
Sse9I AATT 6 cut(s) 61, 533, 686, 752, 848, 1108
SsiI CCGC 6 cut(s) 7, 204, 831, 839, 866, 1049
SspMI CTAG 2 cut(s) 564, 1079
StyD4I CCNGG 8 cut(s) 16, 96, 106, 209, 861, 1035, 1051, 1069
TaaI ACNGT 8 cut(s) 88, 357, 416, 548, 580, 986, 1063, 1144
TaiI ACGT 4 cut(s) 296, 450, 806, 1135
TaqI TCGA 3 cut(s) 431, 764, 1182
TasI AATT 6 cut(s) 61, 533, 686, 752, 848, 1108
TatI WGTACW 3 cut(s) 575, 981, 1002
TauI GCSGC 1 cut(s) 206
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TscAI CASTG 7 cut(s) 275, 287, 500, 553, 700, 813, 942
TseI GCWGC 3 cut(s) 206, 407, 1209
TspDTI ATGAA 2 cut(s) 26, 942
TspGWI ACGGA 6 cut(s) 35, 42, 159, 320, 782, 1020
TspRI CASTG 7 cut(s) 275, 287, 500, 553, 700, 813, 942
VpaK11BI GGWCC 3 cut(s) 43, 231, 646
XapI RAATTY 3 cut(s) 533, 752, 848
XhoI CTCGAG 1 cut(s) 1181
XmiI GTMKAC 1 cut(s) 764
XspI CTAG 2 cut(s) 564, 1079
ZrmI AGTACT 1 cut(s) 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.