Rmu_sc0001350.1_g000004

Chaperone protein dnaJ 20, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001350.1
OG0016939
Physical Location & Seq
Forward (+)
42491 .. 42874
384 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 384 bp
atggatgacataaagagagcttacagaagcatggctctccggtaccaccctgacgttgtggatcatcgagctgatgactcttcgatgaaggagtcaactcagttgtttgttcagctgaatgaggcatacaagacgctgtcggatcccgtgttgcgtgaggagtacgattatgaattgggtttgacagcgaattcatcatgcagttcctcttgtaggagtactacctataaattcaggaaggatatgagtactgttggaccaaatttgcaatggcgacaacagattctcgagttgaagagaagatcaaacggccgtatggcaggaaaggaaggatcctgggctagcagagtcaggagggaagaacatgtttcaaccatggagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

127

Amino Acids

14.8

Weight (kDa)

8.58

Isoelectric Point (pI)

63.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 2 - 56 5.3e-14 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016939)

Species Orthologous Gene IDs
malus_domestica MD08G1221200.v1.1
prunus_persica Prupe.1G558200_v2.0.a1
pyrus_communis pycom15g36670
rosa_chinensis RchiOBHm_Chr7g0226211
rosa_laevigata RLG00000001758
rosa_multiflora Rmu_sc0001350.1_g000004 Rmu_sc0013267.1_g000006
rosa_roxburghii Rroxscaffold_3G00233120
rosa_rugosa Rorug07G0235700
rosa_samantha Rh7AG382600 Rh7BG369800 Rh7CG402100 Rh7DG381000
rosa_wichuraiana Rw7G032060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 42
AccB1I GGYRCC 1 cut(s) 42
AclWI GGATC 5 cut(s) 69, 137, 150, 327, 340
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 3 cut(s) 190, 230, 262
AfaI GTAC 4 cut(s) 44, 164, 220, 250
AfiI CCNNNNNNNGG 1 cut(s) 213
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 2 cut(s) 295, 372
AjnI CCWGG 1 cut(s) 335
AluBI AGCT 3 cut(s) 20, 71, 115
AluI AGCT 3 cut(s) 20, 71, 115
AlwI GGATC 5 cut(s) 69, 137, 150, 327, 340
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 1 cut(s) 310
ApoI RAATTY 3 cut(s) 190, 230, 262
Asp718I GGTACC 1 cut(s) 42
AspS9I GGNCC 1 cut(s) 257
AsuNHI GCTAGC 1 cut(s) 341
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 257
BamHI GGATCC 2 cut(s) 142, 332
BanI GGYRCC 1 cut(s) 42
BceAI ACGGC 2 cut(s) 297, 325
BciT130I CCWGG 1 cut(s) 337
BfaI CTAG 1 cut(s) 342
BmcAI AGTACT 2 cut(s) 220, 250
Bme1390I CCNGG 1 cut(s) 337
Bme18I GGWCC 1 cut(s) 257
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 257
BmiI GGNNCC 3 cut(s) 44, 144, 334
BmrFI CCNGG 1 cut(s) 337
BmtI GCTAGC 1 cut(s) 345
BsaJI CCNNGG 2 cut(s) 336, 375
BsaWI WCCGGW 1 cut(s) 39
Bsc4I CCNNNNNNNGG 1 cut(s) 213
Bse3DI GCAATG 1 cut(s) 275
BseBI CCWGG 1 cut(s) 337
BseDI CCNNGG 2 cut(s) 336, 375
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 1 cut(s) 213
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 1 cut(s) 113
BseRI GAGGAG 1 cut(s) 173
BseX3I CGGCCG 1 cut(s) 310
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 1 cut(s) 312
BshNI GGYRCC 1 cut(s) 42
BsiEI CGRYCG 1 cut(s) 313
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 1 cut(s) 40
BslI CCNNNNNNNGG 1 cut(s) 213
BsnI GGCC 1 cut(s) 312
BsoBI CYCGRG 1 cut(s) 287
Bsp143I GATC 4 cut(s) 61, 142, 302, 332
Bsp19I CCATGG 1 cut(s) 375
BspANI GGCC 1 cut(s) 312
BspCNI CTCAG 1 cut(s) 112
BspLI GGNNCC 3 cut(s) 44, 144, 334
BspOI GCTAGC 1 cut(s) 345
BspPI GGATC 5 cut(s) 69, 137, 150, 327, 340
BspT107I GGYRCC 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 336, 375
BssMI GATC 4 cut(s) 61, 142, 302, 332
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 1 cut(s) 253
Bst6I CTCTTC 2 cut(s) 85, 290
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 1 cut(s) 99
BstDSI CCRYGG 1 cut(s) 375
BstENI CCTNNNNNAGG 1 cut(s) 211
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 4 cut(s) 64, 145, 305, 335
BstMBI GATC 4 cut(s) 61, 142, 302, 332
BstMCI CGRYCG 1 cut(s) 313
BstNI CCWGG 1 cut(s) 337
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 335
BstX2I RGATCY 2 cut(s) 142, 332
BstYI RGATCY 2 cut(s) 142, 332
BstZI CGGCCG 1 cut(s) 310
BsuRI GGCC 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 1 cut(s) 10
Cac8I GCNNGC 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 257
CseI GACGC 1 cut(s) 142
Csp6I GTAC 4 cut(s) 43, 163, 219, 249
CviAII CATG 4 cut(s) 31, 198, 365, 376
CviJI RGCY 6 cut(s) 20, 35, 71, 115, 312, 341
CviKI_1 RGCY 6 cut(s) 20, 35, 71, 115, 312, 341
CviQI GTAC 4 cut(s) 43, 163, 219, 249
DdeI CTNAG 1 cut(s) 99
DpnI GATC 4 cut(s) 63, 144, 304, 334
DpnII GATC 4 cut(s) 61, 142, 302, 332
EaeI YGGCCR 1 cut(s) 310
EagI CGGCCG 1 cut(s) 310
Eam1104I CTCTTC 2 cut(s) 85, 290
EarI CTCTTC 2 cut(s) 85, 290
EclXI CGGCCG 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 375
Eco47I GGWCC 1 cut(s) 257
Eco52I CGGCCG 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 287
EcoNI CCTNNNNNAGG 1 cut(s) 211
EcoRI GAATTC 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 335
EcoT14I CCWWGG 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 4 cut(s) 34, 201, 368, 379
FatI CATG 4 cut(s) 30, 197, 364, 375
FokI GGATG 1 cut(s) 17
FspBI CTAG 1 cut(s) 342
HaeIII GGCC 1 cut(s) 312
HapII CCGG 1 cut(s) 40
HgaI GACGC 1 cut(s) 142
Hin1II CATG 4 cut(s) 34, 201, 368, 379
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
HinfI GANTC 4 cut(s) 77, 92, 283, 348
HpaII CCGG 1 cut(s) 40
Hpy166II GTNNAC 1 cut(s) 96
Hpy188I TCNGA 1 cut(s) 142
Hpy188III TCNNGA 3 cut(s) 235, 287, 352
Hpy8I GTNNAC 1 cut(s) 96
HpyAV CCTTC 3 cut(s) 82, 232, 323
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 2 cut(s) 201, 268
HpyF3I CTNAG 1 cut(s) 99
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 4 cut(s) 34, 201, 368, 379
KpnI GGTACC 1 cut(s) 46
Kzo9I GATC 4 cut(s) 61, 142, 302, 332
LpnPI CCDG 7 cut(s) 53, 63, 220, 306, 322, 337, 349
MaeI CTAG 1 cut(s) 342
MaeII ACGT 1 cut(s) 54
MalI GATC 4 cut(s) 63, 144, 304, 334
MboI GATC 4 cut(s) 61, 142, 302, 332
MboII GAAGA 4 cut(s) 72, 307, 312, 371
MflI RGATCY 2 cut(s) 142, 332
MluCI AATT 4 cut(s) 173, 190, 230, 262
MlyI GAGTC 3 cut(s) 71, 101, 357
MmeI TCCRAC 2 cut(s) 120, 235
MnlI CCTC 4 cut(s) 115, 151, 217, 348
MspA1I CMGCKG 1 cut(s) 115
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 1 cut(s) 337
MvaI CCWGG 1 cut(s) 337
NcoI CCATGG 1 cut(s) 375
NdeII GATC 4 cut(s) 61, 142, 302, 332
NheI GCTAGC 1 cut(s) 341
NlaIII CATG 4 cut(s) 34, 201, 368, 379
NlaIV GGNNCC 3 cut(s) 44, 144, 334
NspI RCATGY 1 cut(s) 368
PaeR7I CTCGAG 1 cut(s) 287
PciI ACATGT 1 cut(s) 364
PfeI GAWTC 1 cut(s) 283
PflFI GACNNNGTC 1 cut(s) 136
PleI GAGTC 3 cut(s) 71, 100, 356
PpsI GAGTC 3 cut(s) 71, 100, 356
PscI ACATGT 1 cut(s) 364
Psp6I CCWGG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 335
PspN4I GGNNCC 3 cut(s) 44, 144, 334
PspPI GGNCC 1 cut(s) 257
PsuI RGATCY 2 cut(s) 142, 332
PsyI GACNNNGTC 1 cut(s) 136
PvuII CAGCTG 1 cut(s) 115
RsaI GTAC 4 cut(s) 44, 164, 220, 250
RsaNI GTAC 4 cut(s) 43, 163, 219, 249
Sau3AI GATC 4 cut(s) 61, 142, 302, 332
Sau96I GGNCC 1 cut(s) 257
ScaI AGTACT 2 cut(s) 220, 250
SchI GAGTC 3 cut(s) 71, 101, 357
ScrFI CCNGG 1 cut(s) 337
SetI ASST 5 cut(s) 22, 57, 73, 117, 227
Sfr274I CTCGAG 1 cut(s) 287
SinI GGWCC 1 cut(s) 257
SlaI CTCGAG 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 4 cut(s) 173, 190, 230, 262
SspMI CTAG 1 cut(s) 342
StyD4I CCNGG 1 cut(s) 335
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 1 cut(s) 253
TaiI ACGT 1 cut(s) 57
TaqI TCGA 3 cut(s) 67, 83, 288
TasI AATT 4 cut(s) 173, 190, 230, 262
TatI WGTACW 2 cut(s) 218, 248
TfiI GAWTC 1 cut(s) 283
TspDTI ATGAA 3 cut(s) 101, 183, 186
Tth111I GACNNNGTC 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 257
XagI CCTNNNNNAGG 1 cut(s) 211
XapI RAATTY 3 cut(s) 190, 230, 262
XceI RCATGY 1 cut(s) 368
XcmI CCANNNNNNNNNTGG 1 cut(s) 267
XhoI CTCGAG 1 cut(s) 287
XspI CTAG 1 cut(s) 342
ZrmI AGTACT 2 cut(s) 220, 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.