Rmu_sc0001715.1_g000010

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001715.1
OG0018192
Physical Location & Seq
Forward (+)
27862 .. 29233
1372 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 813 bp
atgagtagtgagggggaaagcttttccaattgggttgacatggatattgatgatgataatgaatctgttgatttgactagcatacataatctgggagaagcttttttgaaggatttctgtaaggaggcatcagtttcattcttcaaccagtatggcctcattagtcaccaaataaactcgtacaatgacttcattaaaaatggtatacaaagagtctttgatagttttgatgagattatagttgagcctgggtatgacccatcaaagaaggggaatgcagaatggcaatatgcttccatcaggtttgggaaggttaccctcattcgacaaagtttttggggcggtgctgtgaatgataaggagtataatatgctacctaggcatgctcggcttcaaaatatgacatactcagccaagatcaaagtgaacgttactgttgaggtatatactcaaaatcttgtcagaagtgaaaagtttaaaactgggaaagaacaatgtcttgacaagcagattgtgactacagagagtagagatgtctttattggtaagctcccagtgatggtgaagtctgacttgtgttggatgaaagacgagaaagctgactgtgaatttgaccatggaggttattttataatcaagggtgcagagaagacctttattgcacaagagcagaactgtctgaagagacttctaattacaaataatcaggggatcacagtagcatataggtctgaggtaaggcggcatagactactaataatattatcagggatttctcaacttgaaaacattgaaggagtagagaaagtttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

270

Amino Acids

30.97

Weight (kDa)

5.51

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_1 PF04563 52 - 224 5.5e-29 RNA polymerase beta subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 632
AccI GTMKAC 1 cut(s) 205
AciI CCGC 2 cut(s) 342, 742
AclI AACGTT 1 cut(s) 429
AclWI GGATC 1 cut(s) 719
AcsI RAATTY 1 cut(s) 608
AcuI CTGAAG 1 cut(s) 701
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 1 cut(s) 559
AgsI TTSAA 5 cut(s) 109, 145, 395, 785, 794
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 4 cut(s) 21, 101, 550, 599
AluI AGCT 4 cut(s) 21, 101, 550, 599
Alw26I GTCTC 1 cut(s) 679
AlwI GGATC 1 cut(s) 719
AoxI GGCC 1 cut(s) 154
ApoI RAATTY 1 cut(s) 608
ArsI GACNNNNNNTTYG 4 cut(s) 318, 350, 593, 625
Asp700I GAANNNNTTC 1 cut(s) 113
AspA2I CCTAGG 1 cut(s) 377
AsuHPI GGTGA 2 cut(s) 158, 574
AvrII CCTAGG 1 cut(s) 377
BarI GAAGNNNNNNTAC 2 cut(s) 173, 205
BbsI GAAGAC 1 cut(s) 656
BccI CCATC 3 cut(s) 268, 305, 553
BciT130I CCWGG 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 679
BfaI CTAG 2 cut(s) 78, 378
BfmI CTRYAG 1 cut(s) 519
BisI GCNGC 1 cut(s) 743
BlnI CCTAGG 1 cut(s) 377
BlsI GCNGC 1 cut(s) 744
Bme1390I CCNGG 1 cut(s) 249
BmrFI CCNGG 1 cut(s) 249
BmrI ACTGGG 2 cut(s) 492, 548
BmsI GCATC 1 cut(s) 137
BmuI ACTGGG 2 cut(s) 492, 548
BpiI GAAGAC 1 cut(s) 656
BsaJI CCNNGG 3 cut(s) 248, 377, 616
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse1I ACTGG 3 cut(s) 148, 487, 554
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 3 cut(s) 248, 377, 616
BseGI GGATG 1 cut(s) 588
BseLI CCNNNNNNNGG 1 cut(s) 559
BseMII CTCAG 2 cut(s) 423, 723
BseNI ACTGG 3 cut(s) 148, 487, 554
BsgI GTGCAG 1 cut(s) 663
BshFI GGCC 1 cut(s) 156
BslI CCNNNNNNNGG 1 cut(s) 559
BsmAI GTCTC 1 cut(s) 679
BsmI GAATGC 1 cut(s) 280
BsnI GGCC 1 cut(s) 156
Bsp143I GATC 2 cut(s) 417, 711
Bsp19I CCATGG 1 cut(s) 616
BspACI CCGC 2 cut(s) 342, 742
BspANI GGCC 1 cut(s) 156
BspCNI CTCAG 2 cut(s) 422, 724
BspPI GGATC 1 cut(s) 719
BsrI ACTGG 3 cut(s) 148, 487, 554
BssECI CCNNGG 3 cut(s) 248, 377, 616
BssMI GATC 2 cut(s) 417, 711
BssNAI GTATAC 1 cut(s) 206
BssT1I CCWWGG 2 cut(s) 377, 616
Bst1107I GTATAC 1 cut(s) 206
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 4 cut(s) 436, 605, 677, 718
Bst6I CTCTTC 1 cut(s) 677
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 2 cut(s) 409, 732
BstDSI CCRYGG 1 cut(s) 616
BstEII GGTNACC 1 cut(s) 313
BstF5I GGATG 1 cut(s) 588
BstKTI GATC 2 cut(s) 420, 714
BstMAI GTCTC 1 cut(s) 679
BstMBI GATC 2 cut(s) 417, 711
BstMWI GCNNNNNNNGC 2 cut(s) 379, 388
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 1 cut(s) 386
BstPI GGTNACC 1 cut(s) 313
BstSCI CCNGG 1 cut(s) 247
BstSFI CTRYAG 1 cut(s) 519
BstV2I GAAGAC 1 cut(s) 656
BstZ17I GTATAC 1 cut(s) 206
BsuRI GGCC 1 cut(s) 156
BtgI CCRYGG 1 cut(s) 616
BtsCI GGATG 1 cut(s) 588
BtsIMutI CAGTG 1 cut(s) 561
Cac8I GCNNGC 1 cut(s) 384
Csp6I GTAC 1 cut(s) 181
CviAII CATG 3 cut(s) 40, 383, 617
CviJI RGCY 8 cut(s) 21, 101, 156, 247, 391, 413, 550, 599
CviKI_1 RGCY 8 cut(s) 21, 101, 156, 247, 391, 413, 550, 599
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 2 cut(s) 409, 732
DpnI GATC 2 cut(s) 419, 713
DpnII GATC 2 cut(s) 417, 711
DraI TTTAAA 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 677
EarI CTCTTC 1 cut(s) 677
Eco130I CCWWGG 2 cut(s) 377, 616
Eco57I CTGAAG 1 cut(s) 701
Eco91I GGTNACC 1 cut(s) 313
EcoO65I GGTNACC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 247
EcoT14I CCWWGG 2 cut(s) 377, 616
ErhI CCWWGG 2 cut(s) 377, 616
FaeI CATG 3 cut(s) 43, 386, 620
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FatI CATG 3 cut(s) 39, 382, 616
FblI GTMKAC 1 cut(s) 205
Fnu4HI GCNGC 1 cut(s) 743
FokI GGATG 1 cut(s) 595
Fsp4HI GCNGC 1 cut(s) 743
FspBI CTAG 2 cut(s) 78, 378
GluI GCNGC 1 cut(s) 743
HaeIII GGCC 1 cut(s) 156
Hin1II CATG 3 cut(s) 43, 386, 620
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HindIII AAGCTT 2 cut(s) 19, 99
HinfI GANTC 2 cut(s) 62, 213
HphI GGTGA 2 cut(s) 158, 574
Hpy166II GTNNAC 3 cut(s) 37, 206, 427
Hpy188I TCNGA 4 cut(s) 464, 571, 681, 733
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 3 cut(s) 37, 206, 427
HpyAV CCTTC 4 cut(s) 103, 262, 304, 788
HpyCH4III ACNGT 4 cut(s) 436, 605, 677, 718
HpyCH4IV ACGT 1 cut(s) 429
HpyCH4V TGCA 3 cut(s) 278, 644, 662
HpyF10VI GCNNNNNNNGC 2 cut(s) 379, 388
HpyF3I CTNAG 2 cut(s) 409, 732
HpySE526I ACGT 1 cut(s) 429
Hsp92II CATG 3 cut(s) 43, 386, 620
Kzo9I GATC 2 cut(s) 417, 711
LmnI GCTCC 1 cut(s) 555
LpnPI CCDG 9 cut(s) 77, 161, 234, 261, 286, 468, 567, 692, 753
LweI GCATC 1 cut(s) 137
MaeI CTAG 2 cut(s) 78, 378
MaeII ACGT 1 cut(s) 429
MaeIII GTNAC 4 cut(s) 164, 313, 430, 514
MalI GATC 2 cut(s) 419, 713
MboI GATC 2 cut(s) 417, 711
MboII GAAGA 3 cut(s) 133, 661, 694
MfeI CAATTG 1 cut(s) 28
MluCI AATT 3 cut(s) 28, 608, 693
MlyI GAGTC 1 cut(s) 222
MmeI TCCRAC 1 cut(s) 560
MnlI CCTC 7 cut(s) 4, 118, 167, 329, 433, 614, 727
MroXI GAANNNNTTC 1 cut(s) 113
MseI TTAA 2 cut(s) 195, 477
MspR9I CCNGG 1 cut(s) 249
MunI CAATTG 1 cut(s) 28
Mva1269I GAATGC 1 cut(s) 280
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 2 cut(s) 379, 388
NcoI CCATGG 1 cut(s) 616
NdeII GATC 2 cut(s) 417, 711
NlaIII CATG 3 cut(s) 43, 386, 620
NmeAIII GCCGAG 1 cut(s) 367
NmuCI GTSAC 2 cut(s) 164, 514
NspI RCATGY 1 cut(s) 386
PaeI GCATGC 1 cut(s) 386
PctI GAATGC 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 113
PfeI GAWTC 1 cut(s) 62
PkrI GCNGC 1 cut(s) 744
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PsiI TTATAA 1 cut(s) 632
Psp1406I AACGTT 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 247
PspEI GGTNACC 1 cut(s) 313
PspGI CCWGG 1 cut(s) 247
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
SaqAI TTAA 2 cut(s) 195, 477
SatI GCNGC 1 cut(s) 743
Sau3AI GATC 2 cut(s) 417, 711
SchI GAGTC 1 cut(s) 222
ScrFI CCNGG 1 cut(s) 249
SfaNI GCATC 1 cut(s) 137
SfcI CTRYAG 1 cut(s) 519
SphI GCATGC 1 cut(s) 386
Sse9I AATT 3 cut(s) 28, 608, 693
SsiI CCGC 2 cut(s) 342, 742
SspI AATATT 1 cut(s) 762
SspMI CTAG 2 cut(s) 78, 378
StyD4I CCNGG 1 cut(s) 247
StyI CCWWGG 2 cut(s) 377, 616
TaaI ACNGT 4 cut(s) 436, 605, 677, 718
TaiI ACGT 1 cut(s) 432
TaqI TCGA 1 cut(s) 325
TasI AATT 3 cut(s) 28, 608, 693
TauI GCSGC 1 cut(s) 745
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 2 cut(s) 195, 477
Tru9I TTAA 2 cut(s) 195, 477
TscAI CASTG 1 cut(s) 561
TseFI GTSAC 2 cut(s) 164, 514
Tsp45I GTSAC 2 cut(s) 164, 514
TspDTI ATGAA 4 cut(s) 75, 126, 181, 599
TspRI CASTG 1 cut(s) 561
XapI RAATTY 1 cut(s) 608
XceI RCATGY 1 cut(s) 386
XmaJI CCTAGG 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 2 cut(s) 78, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.