Rmu_sc0001754.1_g000007

Mannose-1-phosphate guanyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001754.1
OG0007684
Physical Location & Seq
Reverse (-)
34348 .. 38190
3843 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1506 bp
atggtcccacaccgctccttcccttgtgccccaaaaagtaatgagaagccaaaacggaaacttcgttggcagtggcgcagtgcttatcagcagcgaggagcggtgatgtttctaccattttcggcatataccgtatcaacccgtatattcaccgacactcactttttactgttcattaaggttgttgaaggctctggttttgtagcagacaaggggttgttgttggctttggggatctggatttgtgttaagagccagatggggagctcagaggagaaagttgttgctgtgatcatggtgggtgggcccactaaaggcactagattccggccactgtcattgaatattccgaagccactttttcctttagccggacaaccaatggttcatcatccaatttctgcttgtaaaaagattcctaacctggggcaaatctttctcattggtttctatgaggagcgtgagttatcattatatgtatccgcaatctctaatgagcttaaggtccccgttagatatttgagggaagacaggccacatggttcagctggtggactttataacttcagagatctgatcatggaagacagcccatcacacattttcttgctgaactgtgatgtttgctgcagttttccgcttccagaaatgcttgaggctcacagaaaatatggtgggatgggaacaatcctggtaatcaaggtttctgctgagtcagcaagccagtttggggaacttgtagctgatccagtcaccaatgaactgctgcattacacagagaagcctgagacttttgtcagtgaccggataaattgtggtgtctacatattcacaccagatatttttaatgccatagaggatgtttcctctcagcggaaggacggagctgacttaaggcgtctatccagttttgaagccctgcactccgcaacaaggagtgcatccacacattttgtaaggttggatcaagatatcctatcccctcttgcaggaaagaagcagttatatacatatgaaaccatggacttctgggaacagattaaaactcctggaatgtccttgaaatgttctgggttgtatctttcacaattcagagtcaccagtccccatctgttggtgagtggggatggtacaaagagtgccagtatttctggtgatgtttacatccatccatcagcaaaagtacacccaagtgctaaaattggccctaatgtctcaatatctgcaaatgctcgtataggagctggtgtgaggctcataagttgtatcatacttgacaatgtcgaaattaaggaaaatgcagttgttatgcatgcaattattggatggaaatcttctattgggaaatggtctcgtatccaggctagtgggaattacaattctaagctcgggatcacaatccttggtgaagatgtgacagttgaagatgaagtcgtggttatcaacagcattgtcctcccccataagacgctcaatctgagtgttcaggaggaaataattctgtga

Protein Analysis

501

Amino Acids

55.45

Weight (kDa)

8.58

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 96 - 291 2.9e-31 Nucleotidyl transferase
LbH_EIF2B PF25084 379 - 455 1.3e-08 EIF2B subunit epsilon LbH domain
Hexapep PF00132 381 - 414 1.6e-06 Bacterial transferase hexapeptide (six repeats)
GMPPB_C PF25087 383 - 500 9.3e-39 GMPPB C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 561
AccB7I CCANNNNNTGG 1 cut(s) 1114
AccBSI CCGCTC 2 cut(s) 15, 101
AccI GTMKAC 1 cut(s) 822
AciI CCGC 6 cut(s) 13, 101, 483, 638, 874, 927
AclWI GGATC 4 cut(s) 242, 740, 972, 1400
AcoI YGGCCR 1 cut(s) 329
AcuI CTGAAG 1 cut(s) 550
AcyI GRCGYC 1 cut(s) 898
AfaI GTAC 2 cut(s) 1132, 1185
AfiI CCNNNNNNNGG 8 cut(s) 314, 371, 425, 730, 873, 933, 989, 1114
AflII CTTAAG 2 cut(s) 500, 892
AgsI TTSAA 5 cut(s) 188, 343, 914, 1063, 1424
AjnI CCWGG 4 cut(s) 423, 690, 1048, 1359
AleI CACNNNNGTG 1 cut(s) 1191
AluBI AGCT 7 cut(s) 267, 499, 548, 743, 887, 1244, 1387
AluI AGCT 7 cut(s) 267, 499, 548, 743, 887, 1244, 1387
Alw21I GWGCWC 1 cut(s) 269
Alw26I GTCTC 3 cut(s) 782, 1219, 1356
AlwI GGATC 4 cut(s) 242, 740, 972, 1400
Ama87I CYCGRG 1 cut(s) 1388
AoxI GGCC 4 cut(s) 305, 329, 533, 1204
ApaI GGGCCC 1 cut(s) 309
ApeKI GCWGC 3 cut(s) 91, 627, 766
Asp700I GAANNNNTTC 1 cut(s) 1497
AspLEI GCGC 1 cut(s) 78
AspS9I GGNCC 5 cut(s) 4, 305, 306, 505, 1205
AsuHPI GGTGA 7 cut(s) 115, 142, 745, 1090, 1129, 1166, 1418
AvaI CYCGRG 1 cut(s) 1388
AvaII GGWCC 2 cut(s) 4, 505
BaeGI GKGCMC 2 cut(s) 31, 309
BanII GRGCYC 2 cut(s) 269, 309
BbsI GAAGAC 2 cut(s) 534, 591
Bbv12I GWGCWC 1 cut(s) 269
BbvI GCAGC 3 cut(s) 103, 614, 753
BccI CCATC 8 cut(s) 253, 601, 673, 1116, 1121, 1176, 1180, 1320
BciT130I CCWGG 4 cut(s) 425, 692, 1050, 1361
BciVI GTATCC 2 cut(s) 490, 1367
BclI TGATCA 2 cut(s) 291, 576
BcoDI GTCTC 3 cut(s) 782, 1219, 1356
BfaI CTAG 2 cut(s) 321, 1365
BfmI CTRYAG 1 cut(s) 628
BfrI CTTAAG 2 cut(s) 500, 892
BfuI GTATCC 2 cut(s) 490, 1367
BglII AGATCT 1 cut(s) 571
BisI GCNGC 3 cut(s) 92, 628, 767
BlsI GCNGC 3 cut(s) 93, 629, 768
Bme1390I CCNGG 4 cut(s) 425, 692, 1050, 1361
Bme18I GGWCC 2 cut(s) 4, 505
BmeT110I CYCGRG 1 cut(s) 1388
BmgT120I GGNCC 5 cut(s) 4, 305, 306, 505, 1205
BmiI GGNNCC 3 cut(s) 6, 307, 507
BmrFI CCNGG 4 cut(s) 425, 692, 1050, 1361
BmsI GCATC 1 cut(s) 950
BoxI GACNNNNGTC 1 cut(s) 794
BpiI GAAGAC 2 cut(s) 534, 591
BpuEI CTTGAG 1 cut(s) 674
BsaBI GATNNNNATC 2 cut(s) 1330, 1397
BsaHI GRCGYC 1 cut(s) 898
BsaI GGTCTC 1 cut(s) 1356
BsaJI CCNNGG 3 cut(s) 424, 1020, 1402
BsaWI WCCGGW 1 cut(s) 804
BsaXI ACNNNNNCTCC 4 cut(s) 449, 479, 1233, 1263
Bsc4I CCNNNNNNNGG 8 cut(s) 314, 371, 425, 730, 873, 933, 989, 1114
Bse1I ACTGG 5 cut(s) 724, 749, 906, 1101, 1143
Bse8I GATNNNNATC 2 cut(s) 1330, 1397
BseBI CCWGG 4 cut(s) 425, 692, 1050, 1361
BseDI CCNNGG 3 cut(s) 424, 1020, 1402
BseGI GGATG 8 cut(s) 391, 684, 865, 941, 1132, 1164, 1168, 1331
BseJI GATNNNNATC 2 cut(s) 1330, 1397
BseLI CCNNNNNNNGG 8 cut(s) 314, 371, 425, 730, 873, 933, 989, 1114
BseMII CTCAG 5 cut(s) 282, 702, 777, 884, 1469
BseNI ACTGG 5 cut(s) 724, 749, 906, 1101, 1143
BseRI GAGGAG 3 cut(s) 111, 287, 470
BseSI GKGCMC 2 cut(s) 31, 309
BseXI GCAGC 3 cut(s) 103, 614, 753
BsgI GTGCAG 1 cut(s) 905
BshFI GGCC 4 cut(s) 307, 331, 535, 1206
BsiHKAI GWGCWC 1 cut(s) 269
BsiHKCI CYCGRG 1 cut(s) 1388
BsiSI CCGG 3 cut(s) 328, 372, 805
BslFI GGGAC 2 cut(s) 491, 1089
BslI CCNNNNNNNGG 8 cut(s) 314, 371, 425, 730, 873, 933, 989, 1114
BsmAI GTCTC 3 cut(s) 782, 1219, 1356
BsmFI GGGAC 2 cut(s) 491, 1089
BsnI GGCC 4 cut(s) 307, 331, 535, 1206
Bso31I GGTCTC 1 cut(s) 1356
BsoBI CYCGRG 1 cut(s) 1388
Bsp120I GGGCCC 1 cut(s) 305
Bsp1286I GDGCHC 3 cut(s) 31, 269, 309
Bsp143I GATC 7 cut(s) 234, 291, 571, 576, 745, 964, 1392
Bsp19I CCATGG 1 cut(s) 1020
BspACI CCGC 6 cut(s) 13, 101, 483, 638, 874, 927
BspANI GGCC 4 cut(s) 307, 331, 535, 1206
BspCNI CTCAG 5 cut(s) 281, 703, 778, 883, 1470
BspLI GGNNCC 3 cut(s) 6, 307, 507
BspMAI CTGCAG 1 cut(s) 632
BspPI GGATC 4 cut(s) 242, 740, 972, 1400
BspTI CTTAAG 2 cut(s) 500, 892
BspTNI GGTCTC 1 cut(s) 1356
BsrBI CCGCTC 2 cut(s) 15, 101
BsrI ACTGG 5 cut(s) 724, 749, 906, 1101, 1143
BssECI CCNNGG 3 cut(s) 424, 1020, 1402
BssMI GATC 7 cut(s) 234, 291, 571, 576, 745, 964, 1392
BssNI GRCGYC 1 cut(s) 898
BssT1I CCWWGG 2 cut(s) 1020, 1402
Bst2UI CCWGG 4 cut(s) 425, 692, 1050, 1361
Bst4CI ACNGT 5 cut(s) 133, 171, 336, 617, 1420
BstACI GRCGYC 1 cut(s) 898
BstAFI CTTAAG 2 cut(s) 500, 892
BstC8I GCNNGC 2 cut(s) 721, 1314
BstDEI CTNAG 6 cut(s) 268, 711, 786, 870, 1383, 1478
BstDSI CCRYGG 1 cut(s) 1020
BstENI CCTNNNNNAGG 1 cut(s) 987
BstF5I GGATG 8 cut(s) 391, 684, 865, 941, 1132, 1164, 1168, 1331
BstHHI GCGC 1 cut(s) 78
BstKTI GATC 7 cut(s) 237, 294, 574, 579, 748, 967, 1395
BstMAI GTCTC 3 cut(s) 782, 1219, 1356
BstMBI GATC 7 cut(s) 234, 291, 571, 576, 745, 964, 1392
BstMWI GCNNNNNNNGC 1 cut(s) 716
BstNI CCWGG 4 cut(s) 425, 692, 1050, 1361
BstNSI RCATGY 1 cut(s) 1316
BstPAI GACNNNNGTC 1 cut(s) 794
BstSCI CCNGG 4 cut(s) 423, 690, 1048, 1359
BstSFI CTRYAG 1 cut(s) 628
BstSLI GKGCMC 2 cut(s) 31, 309
BstV1I GCAGC 3 cut(s) 103, 614, 753
BstV2I GAAGAC 2 cut(s) 534, 591
BstX2I RGATCY 2 cut(s) 234, 571
BstXI CCANNNNNNTGG 1 cut(s) 1367
BstYI RGATCY 2 cut(s) 234, 571
BsuI GTATCC 2 cut(s) 490, 1367
BsuRI GGCC 4 cut(s) 307, 331, 535, 1206
BtgI CCRYGG 1 cut(s) 1020
BtsCI GGATG 8 cut(s) 391, 684, 865, 941, 1132, 1164, 1168, 1331
BtsI GCAGTG 2 cut(s) 77, 85
BtsIMutI CAGTG 4 cut(s) 77, 85, 332, 805
Cac8I GCNNGC 2 cut(s) 721, 1314
CfoI GCGC 1 cut(s) 78
Cfr13I GGNCC 5 cut(s) 4, 305, 306, 505, 1205
CseI GACGC 2 cut(s) 887, 1477
Csp6I GTAC 2 cut(s) 1131, 1184
CspCI CAANNNNNGTGG 4 cut(s) 321, 356, 934, 969
CviAII CATG 5 cut(s) 295, 539, 580, 1021, 1313
CviQI GTAC 2 cut(s) 1131, 1184
DdeI CTNAG 6 cut(s) 268, 711, 786, 870, 1383, 1478
DpnI GATC 7 cut(s) 236, 293, 573, 578, 747, 966, 1394
DpnII GATC 7 cut(s) 234, 291, 571, 576, 745, 964, 1392
EaeI YGGCCR 1 cut(s) 329
Ecl136II GAGCTC 1 cut(s) 267
Eco130I CCWWGG 2 cut(s) 1020, 1402
Eco24I GRGCYC 2 cut(s) 269, 309
Eco31I GGTCTC 1 cut(s) 1356
Eco32I GATATC 1 cut(s) 973
Eco47I GGWCC 2 cut(s) 4, 505
Eco53kI GAGCTC 1 cut(s) 267
Eco57I CTGAAG 1 cut(s) 550
Eco88I CYCGRG 1 cut(s) 1388
EcoICRI GAGCTC 1 cut(s) 267
EcoNI CCTNNNNNAGG 1 cut(s) 987
EcoO109I RGGNCCY 1 cut(s) 505
EcoRII CCWGG 4 cut(s) 423, 690, 1048, 1359
EcoRV GATATC 1 cut(s) 973
EcoT14I CCWWGG 2 cut(s) 1020, 1402
EcoT22I ATGCAT 1 cut(s) 1314
EcoT38I GRGCYC 2 cut(s) 269, 309
ErhI CCWWGG 2 cut(s) 1020, 1402
FaeI CATG 5 cut(s) 298, 542, 583, 1024, 1316
FaqI GGGAC 2 cut(s) 491, 1089
FatI CATG 5 cut(s) 294, 538, 579, 1020, 1312
FauNDI CATATG 1 cut(s) 1012
FbaI TGATCA 2 cut(s) 291, 576
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 3 cut(s) 92, 628, 767
FokI GGATG 8 cut(s) 378, 691, 872, 928, 1139, 1151, 1155, 1338
FriOI GRGCYC 2 cut(s) 269, 309
Fsp4HI GCNGC 3 cut(s) 92, 628, 767
FspBI CTAG 2 cut(s) 321, 1365
GlaI GCGC 1 cut(s) 77
GluI GCNGC 3 cut(s) 92, 628, 767
HaeIII GGCC 4 cut(s) 307, 331, 535, 1206
HapII CCGG 3 cut(s) 328, 372, 805
HgaI GACGC 2 cut(s) 887, 1477
HhaI GCGC 1 cut(s) 78
Hin1I GRCGYC 1 cut(s) 898
Hin1II CATG 5 cut(s) 298, 542, 583, 1024, 1316
Hin6I GCGC 1 cut(s) 76
HinP1I GCGC 1 cut(s) 76
HinfI GANTC 4 cut(s) 324, 415, 713, 1095
HpaII CCGG 3 cut(s) 328, 372, 805
HphI GGTGA 7 cut(s) 115, 142, 745, 1090, 1129, 1166, 1418
Hpy166II GTNNAC 4 cut(s) 554, 823, 1162, 1186
Hpy188I TCNGA 6 cut(s) 271, 351, 569, 576, 1094, 1479
Hpy188III TCNNGA 5 cut(s) 238, 644, 968, 1390, 1487
Hpy8I GTNNAC 4 cut(s) 554, 823, 1162, 1186
HpyAV CCTTC 3 cut(s) 28, 182, 871
HpyCH4III ACNGT 5 cut(s) 133, 171, 336, 617, 1420
HpyCH4V TGCA 9 cut(s) 630, 769, 922, 941, 989, 1226, 1301, 1312, 1316
HpyF10VI GCNNNNNNNGC 1 cut(s) 716
HpyF3I CTNAG 6 cut(s) 268, 711, 786, 870, 1383, 1478
Hsp92I GRCGYC 1 cut(s) 898
Hsp92II CATG 5 cut(s) 298, 542, 583, 1024, 1316
HspAI GCGC 1 cut(s) 76
Ksp22I TGATCA 2 cut(s) 291, 576
Kzo9I GATC 7 cut(s) 234, 291, 571, 576, 745, 964, 1392
LmnI GCTCC 6 cut(s) 20, 98, 264, 457, 884, 1241
Lsp1109I GCAGC 3 cut(s) 103, 614, 753
LweI GCATC 1 cut(s) 950
MaeI CTAG 2 cut(s) 321, 1365
MaeIII GTNAC 4 cut(s) 751, 800, 1096, 1414
MalI GATC 7 cut(s) 236, 293, 573, 578, 747, 966, 1394
MbiI CCGCTC 2 cut(s) 15, 101
MboI GATC 7 cut(s) 234, 291, 571, 576, 745, 964, 1392
MboII GAAGA 5 cut(s) 539, 596, 1326, 1421, 1436
MflI RGATCY 2 cut(s) 234, 571
MhlI GDGCHC 3 cut(s) 31, 269, 309
MluCI AATT 9 cut(s) 396, 811, 1088, 1200, 1287, 1317, 1372, 1378, 1497
MlyI GAGTC 2 cut(s) 722, 1104
MmeI TCCRAC 1 cut(s) 942
Mph1103I ATGCAT 1 cut(s) 1314
MroXI GAANNNNTTC 1 cut(s) 1497
MseI TTAA 7 cut(s) 177, 249, 501, 846, 893, 1041, 1290
MslI CAYNNNNRTG 1 cut(s) 1191
MspA1I CMGCKG 2 cut(s) 548, 874
MspCI CTTAAG 2 cut(s) 500, 892
MspI CCGG 3 cut(s) 328, 372, 805
MspR9I CCNGG 4 cut(s) 425, 692, 1050, 1361
MvaI CCWGG 4 cut(s) 425, 692, 1050, 1361
MwoI GCNNNNNNNGC 1 cut(s) 716
NcoI CCATGG 1 cut(s) 1020
NdeI CATATG 1 cut(s) 1012
NdeII GATC 7 cut(s) 234, 291, 571, 576, 745, 964, 1392
NlaIII CATG 5 cut(s) 298, 542, 583, 1024, 1316
NlaIV GGNNCC 3 cut(s) 6, 307, 507
NmuCI GTSAC 4 cut(s) 751, 800, 1096, 1414
NsiI ATGCAT 1 cut(s) 1314
NspI RCATGY 1 cut(s) 1316
OliI CACNNNNGTG 1 cut(s) 1191
PaeI GCATGC 1 cut(s) 1316
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PdmI GAANNNNTTC 1 cut(s) 1497
PfeI GAWTC 2 cut(s) 324, 415
PflFI GACNNNGTC 1 cut(s) 1280
PflMI CCANNNNNTGG 1 cut(s) 1114
PfoI TCCNGGA 1 cut(s) 1048
PkrI GCNGC 3 cut(s) 93, 629, 768
PleI GAGTC 2 cut(s) 721, 1103
PpsI GAGTC 2 cut(s) 721, 1103
PpuMI RGGWCCY 1 cut(s) 505
PshAI GACNNNNGTC 1 cut(s) 794
PsiI TTATAA 1 cut(s) 561
Psp124BI GAGCTC 1 cut(s) 269
Psp5II RGGWCCY 1 cut(s) 505
Psp6I CCWGG 4 cut(s) 423, 690, 1048, 1359
PspGI CCWGG 4 cut(s) 423, 690, 1048, 1359
PspN4I GGNNCC 3 cut(s) 6, 307, 507
PspOMI GGGCCC 1 cut(s) 305
PspPI GGNCC 5 cut(s) 4, 305, 306, 505, 1205
PspPPI RGGWCCY 1 cut(s) 505
PstI CTGCAG 1 cut(s) 632
PsuI RGATCY 2 cut(s) 234, 571
PsyI GACNNNGTC 1 cut(s) 1280
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 2 cut(s) 1132, 1185
RsaNI GTAC 2 cut(s) 1131, 1184
RseI CAYNNNNRTG 1 cut(s) 1191
SacI GAGCTC 1 cut(s) 269
SaqAI TTAA 7 cut(s) 177, 249, 501, 846, 893, 1041, 1290
SatI GCNGC 3 cut(s) 92, 628, 767
Sau3AI GATC 7 cut(s) 234, 291, 571, 576, 745, 964, 1392
Sau96I GGNCC 5 cut(s) 4, 305, 306, 505, 1205
SchI GAGTC 2 cut(s) 722, 1104
ScrFI CCNGG 4 cut(s) 425, 692, 1050, 1361
SduI GDGCHC 3 cut(s) 31, 269, 309
SfaNI GCATC 1 cut(s) 950
SfcI CTRYAG 1 cut(s) 628
SinI GGWCC 2 cut(s) 4, 505
SmiMI CAYNNNNRTG 1 cut(s) 1191
SmlI CTYRAG 3 cut(s) 500, 653, 892
SmoI CTYRAG 3 cut(s) 500, 653, 892
SphI GCATGC 1 cut(s) 1316
Sse9I AATT 9 cut(s) 396, 811, 1088, 1200, 1287, 1317, 1372, 1378, 1497
SsiI CCGC 6 cut(s) 13, 101, 483, 638, 874, 927
SspI AATATT 1 cut(s) 346
SspMI CTAG 2 cut(s) 321, 1365
SstI GAGCTC 1 cut(s) 269
StyD4I CCNGG 4 cut(s) 423, 690, 1048, 1359
StyI CCWWGG 2 cut(s) 1020, 1402
TaaI ACNGT 5 cut(s) 133, 171, 336, 617, 1420
TaqI TCGA 1 cut(s) 1284
TasI AATT 9 cut(s) 396, 811, 1088, 1200, 1287, 1317, 1372, 1378, 1497
TatI WGTACW 1 cut(s) 1183
TfiI GAWTC 2 cut(s) 324, 415
Tru1I TTAA 7 cut(s) 177, 249, 501, 846, 893, 1041, 1290
Tru9I TTAA 7 cut(s) 177, 249, 501, 846, 893, 1041, 1290
TscAI CASTG 4 cut(s) 77, 85, 339, 805
TseFI GTSAC 4 cut(s) 751, 800, 1096, 1414
TseI GCWGC 3 cut(s) 91, 627, 766
Tsp45I GTSAC 4 cut(s) 751, 800, 1096, 1414
TspDTI ATGAA 5 cut(s) 163, 377, 774, 1029, 1443
TspGWI ACGGA 2 cut(s) 70, 897
TspRI CASTG 4 cut(s) 77, 85, 339, 805
Tth111I GACNNNGTC 1 cut(s) 1280
Van91I CCANNNNNTGG 1 cut(s) 1114
Vha464I CTTAAG 2 cut(s) 500, 892
VpaK11BI GGWCC 2 cut(s) 4, 505
XagI CCTNNNNNAGG 1 cut(s) 987
XceI RCATGY 1 cut(s) 1316
XmiI GTMKAC 1 cut(s) 822
XmnI GAANNNNTTC 1 cut(s) 1497
XspI CTAG 2 cut(s) 321, 1365
Zsp2I ATGCAT 1 cut(s) 1314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.