Rmu_sc0001759.1_g000024

cysteine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001759.1
OG0014007
Physical Location & Seq
Forward (+)
87052 .. 90877
3826 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1494 bp
atggtgcgaggaatgatggtcgaagatgggggtggcaatgaaggaggtggtgctaatgaggatctgcagctcgtgtcctctcccacccacaacctagatccgacaaggattttgatgctgtggccactttccggtccagcccagaaccactgccgccacccatgttctcccatggttgcttttcggtctaacccagcgccttttggcggtgttccagttcgaaccgccgttatggcgcctgtgcgaattacaggcgctatggtcgctgcaatctcagtgaccatggccttgctctctatagtcctctacaagtccaagaagaccaaaccggccttaccctcaaacaaaaatcccagaaatggcctcgttggagcaattggcaacacccctctcattcgcatcaacagcctctctgacgccactggttgtgaaattcttgggaagtgcgagtttttgaacccaggagggagtgtgaaagatagagttgctgtgaaaattattgaagaggctttggaatctggagacctagctccaggtggagtagtcactgagggaagtgctggaagcacagctattagccttgctacagttgctcctgcttatggttgcaaatgccatgtggttatacctgatgacgctgctattgagaagtctcagatacttgaagccctgggagccactgttgagagggtaagaccagtttcaatcacacacagggatcactttgtcaacattgctaggcaacgggcatcagaagcaaatgagttagccttaaagcatagaaaagatgagcaaaagaatggcaaaacccttgagcaagttaatggtttccattatgatgaagagaaacagttgtttccaaggaattgtcctggtggatactttgctgatcaatttgaaaacttggcaaacttccgggcacactatgagggtaccgggcctgagatttgggagcagactggaggaaatgtagatgcatttgtggcggcagcaggaacaggtggtactgttgctggtgcttccaagtttctacaggaaaggaatccaaacatcaaatgtttcctgatagaccctcctggttctggtttgtttaataagattacgaggggagtgatgtacaccaaagaggaggctgaagggcgaaggctaaagaatccatttgacacgataactgaaggaattggaatcaatagactaacaaagaattttctgatggcaaagcttgatggggcttttcgaggcacagataaggaagctgttgaaatgtccaggtttctgatgaagaacgatgggctcttcttggggagttcttcagctatgaattgtgttggagcagttcgagtggcacagtcaattggtcctggtcacactattgtcaccatattgtgtgatagtgggatgagacatctgagcaaattttacaatgctgagtatctgtctaaatttgatttgacacccactgcttgcggattagagttcctgggtgttggataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

497

Amino Acids

53.12

Weight (kDa)

7.57

Isoelectric Point (pI)

39.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 125 - 464 2.9e-65 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55880 AT1G55880
fragaria_vesca FvH4_3g29380 FvH4_3g29380
malus_domestica MD03G1184200.v1.1
prunus_persica Prupe.4G242200_v2.0.a1
pyrus_communis pycom03g13750
rosa_chinensis RchiOBHm_Chr5g0053771
rosa_laevigata RLG00000034902
rosa_multiflora Rmu_sc0001759.1_g000024
rosa_roxburghii Rroxscaffold_1G00026820
rosa_rugosa Rorug05G0282500 Rorug05G0282600 Rorug05G0282700
rosa_samantha Rh5BG364700 Rh5DG379500
rosa_wichuraiana Rw5G033350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 932
AccB1I GGYRCC 2 cut(s) 235, 932
AciI CCGC 5 cut(s) 154, 207, 225, 986, 1467
AclWI GGATC 3 cut(s) 69, 92, 726
AcoI YGGCCR 1 cut(s) 122
AcsI RAATTY 4 cut(s) 432, 1204, 1415, 1442
AcuI CTGAAG 3 cut(s) 1155, 1194, 1296
AcyI GRCGYC 2 cut(s) 236, 417
AfaI GTAC 3 cut(s) 934, 1006, 1118
AfiI CCNNNNNNNGG 5 cut(s) 131, 206, 359, 602, 1082
AgsI TTSAA 6 cut(s) 457, 503, 665, 705, 899, 1262
AjnI CCWGG 8 cut(s) 460, 532, 669, 871, 1075, 1268, 1360, 1479
AluBI AGCT 6 cut(s) 70, 530, 572, 1222, 1256, 1316
AluI AGCT 6 cut(s) 70, 530, 572, 1222, 1256, 1316
Alw26I GTCTC 3 cut(s) 516, 657, 1396
AlwI GGATC 3 cut(s) 69, 92, 726
AoxI GGCC 5 cut(s) 122, 285, 330, 361, 938
ApeKI GCWGC 4 cut(s) 67, 266, 638, 989
ApoI RAATTY 4 cut(s) 432, 1204, 1415, 1442
ArsI GACNNNNNNTTYG 2 cut(s) 94, 126
Asp718I GGTACC 1 cut(s) 932
AspLEI GCGC 3 cut(s) 199, 238, 257
AspS9I GGNCC 3 cut(s) 134, 938, 1358
AsuC2I CCSGG 2 cut(s) 917, 937
AsuHPI GGTGA 1 cut(s) 1369
AsuII TTCGAA 1 cut(s) 220
AvaII GGWCC 2 cut(s) 134, 1358
BaeGI GKGCMC 1 cut(s) 922
BalI TGGCCA 1 cut(s) 124
BanI GGYRCC 2 cut(s) 235, 932
BanII GRGCYC 1 cut(s) 1296
BauI CACGAG 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 326
BbvI GCAGC 4 cut(s) 79, 253, 625, 1001
BccI CCATC 5 cut(s) 10, 20, 1207, 1220, 1283
BceAI ACGGC 1 cut(s) 212
BciT130I CCWGG 8 cut(s) 462, 534, 671, 873, 1077, 1270, 1362, 1481
BciVI GTATCC 1 cut(s) 872
BclI TGATCA 1 cut(s) 889
BcnI CCSGG 2 cut(s) 917, 937
BcoDI GTCTC 3 cut(s) 516, 657, 1396
BfaI CTAG 3 cut(s) 95, 527, 738
BfmI CTRYAG 4 cut(s) 65, 297, 585, 1031
BfoI RGCGCY 3 cut(s) 200, 239, 258
BfuI GTATCC 1 cut(s) 872
BglI GCCNNNNNGGC 1 cut(s) 233
BisI GCNGC 6 cut(s) 68, 154, 267, 639, 987, 990
BlsI GCNGC 6 cut(s) 69, 155, 268, 640, 988, 991
Bme18I GGWCC 2 cut(s) 134, 1358
BmgT120I GGNCC 3 cut(s) 134, 938, 1358
BmiI GGNNCC 3 cut(s) 237, 676, 934
BmsI GCATC 4 cut(s) 105, 408, 758, 964
BpiI GAAGAC 1 cut(s) 326
BpmI CTGGAG 3 cut(s) 516, 540, 981
Bpu14I TTCGAA 1 cut(s) 220
BpuEI CTTGAG 1 cut(s) 833
BpuMI CCSGG 2 cut(s) 917, 937
BsaHI GRCGYC 2 cut(s) 236, 417
BsaI GGTCTC 1 cut(s) 516
BsaJI CCNNGG 7 cut(s) 171, 282, 460, 669, 670, 860, 1480
BsaWI WCCGGW 1 cut(s) 131
BsaXI ACNNNNNCTCC 8 cut(s) 456, 486, 577, 607, 666, 696, 954, 984
Bsc4I CCNNNNNNNGG 5 cut(s) 131, 206, 359, 602, 1082
Bse118I RCCGGY 1 cut(s) 328
Bse1I ACTGG 4 cut(s) 215, 427, 698, 964
Bse3DI GCAATG 2 cut(s) 43, 732
BseBI CCWGG 8 cut(s) 462, 534, 671, 873, 1077, 1270, 1362, 1481
BseDI CCNNGG 7 cut(s) 171, 282, 460, 669, 670, 860, 1480
BseGI GGATG 1 cut(s) 1404
BseLI CCNNNNNNNGG 5 cut(s) 131, 206, 359, 602, 1082
BseMI GCAATG 2 cut(s) 43, 732
BseMII CTCAG 6 cut(s) 288, 540, 668, 933, 1400, 1419
BseNI ACTGG 4 cut(s) 215, 427, 698, 964
BseRI GAGGAG 1 cut(s) 1142
BseSI GKGCMC 1 cut(s) 922
BseXI GCAGC 4 cut(s) 79, 253, 625, 1001
BseYI CCCAGC 1 cut(s) 193
BshFI GGCC 5 cut(s) 124, 287, 332, 363, 940
BshNI GGYRCC 2 cut(s) 235, 932
BsiSI CCGG 4 cut(s) 132, 329, 916, 936
BslI CCNNNNNNNGG 5 cut(s) 131, 206, 359, 602, 1082
BsmAI GTCTC 3 cut(s) 516, 657, 1396
BsnI GGCC 5 cut(s) 124, 287, 332, 363, 940
Bso31I GGTCTC 1 cut(s) 516
Bsp119I TTCGAA 1 cut(s) 220
Bsp1286I GDGCHC 2 cut(s) 922, 1296
Bsp1407I TGTACA 1 cut(s) 1116
Bsp143I GATC 4 cut(s) 61, 97, 718, 889
Bsp19I CCATGG 2 cut(s) 171, 282
BspACI CCGC 5 cut(s) 154, 207, 225, 986, 1467
BspANI GGCC 5 cut(s) 124, 287, 332, 363, 940
BspCNI CTCAG 6 cut(s) 287, 541, 667, 934, 1401, 1420
BspLI GGNNCC 3 cut(s) 237, 676, 934
BspMAI CTGCAG 1 cut(s) 69
BspPI GGATC 3 cut(s) 69, 92, 726
BspQI GCTCTTC 1 cut(s) 1301
BspT104I TTCGAA 1 cut(s) 220
BspT107I GGYRCC 2 cut(s) 235, 932
BspTNI GGTCTC 1 cut(s) 516
BsrDI GCAATG 2 cut(s) 43, 732
BsrFI RCCGGY 1 cut(s) 328
BsrGI TGTACA 1 cut(s) 1116
BsrI ACTGG 4 cut(s) 215, 427, 698, 964
BssAI RCCGGY 1 cut(s) 328
BssECI CCNNGG 7 cut(s) 171, 282, 460, 669, 670, 860, 1480
BssMI GATC 4 cut(s) 61, 97, 718, 889
BssNI GRCGYC 2 cut(s) 236, 417
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 3 cut(s) 171, 282, 860
Bst2BI CACGAG 1 cut(s) 71
Bst2UI CCWGG 8 cut(s) 462, 534, 671, 873, 1077, 1270, 1362, 1481
Bst4CI ACNGT 5 cut(s) 589, 682, 852, 1009, 1350
Bst6I CTCTTC 3 cut(s) 498, 837, 1301
BstACI GRCGYC 2 cut(s) 236, 417
BstAUI TGTACA 1 cut(s) 1116
BstBI TTCGAA 1 cut(s) 220
BstC8I GCNNGC 1 cut(s) 1465
BstDEI CTNAG 6 cut(s) 274, 549, 654, 942, 1409, 1428
BstDSI CCRYGG 2 cut(s) 171, 282
BstF5I GGATG 1 cut(s) 1404
BstH2I RGCGCY 3 cut(s) 200, 239, 258
BstHHI GCGC 3 cut(s) 199, 238, 257
BstKTI GATC 4 cut(s) 64, 100, 721, 892
BstMAI GTCTC 3 cut(s) 516, 657, 1396
BstMBI GATC 4 cut(s) 61, 97, 718, 889
BstMWI GCNNNNNNNGC 7 cut(s) 233, 263, 405, 590, 674, 755, 983
BstNI CCWGG 8 cut(s) 462, 534, 671, 873, 1077, 1270, 1362, 1481
BstSFI CTRYAG 4 cut(s) 65, 297, 585, 1031
BstSLI GKGCMC 1 cut(s) 922
BstV1I GCAGC 4 cut(s) 79, 253, 625, 1001
BstV2I GAAGAC 1 cut(s) 326
BstX2I RGATCY 2 cut(s) 61, 97
BstYI RGATCY 2 cut(s) 61, 97
BsuI GTATCC 1 cut(s) 872
BsuRI GGCC 5 cut(s) 124, 287, 332, 363, 940
BtgI CCRYGG 2 cut(s) 171, 282
BtsCI GGATG 1 cut(s) 1404
BtsI GCAGTG 2 cut(s) 148, 1458
BtsIMutI CAGTG 6 cut(s) 148, 282, 420, 546, 678, 1458
Cac8I GCNNGC 1 cut(s) 1465
CfoI GCGC 3 cut(s) 199, 238, 257
Cfr10I RCCGGY 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 134, 938, 1358
CseI GACGC 2 cut(s) 425, 644
Csp6I GTAC 3 cut(s) 933, 1005, 1117
CviAII CATG 4 cut(s) 162, 172, 283, 617
CviQI GTAC 3 cut(s) 933, 1005, 1117
DdeI CTNAG 6 cut(s) 274, 549, 654, 942, 1409, 1428
DinI GGCGCC 1 cut(s) 237
DpnI GATC 4 cut(s) 63, 99, 720, 891
DpnII GATC 4 cut(s) 61, 97, 718, 889
EaeI YGGCCR 1 cut(s) 122
Eam1104I CTCTTC 3 cut(s) 498, 837, 1301
EarI CTCTTC 3 cut(s) 498, 837, 1301
Eco130I CCWWGG 3 cut(s) 171, 282, 860
Eco24I GRGCYC 1 cut(s) 1296
Eco31I GGTCTC 1 cut(s) 516
Eco47I GGWCC 2 cut(s) 134, 1358
Eco57I CTGAAG 3 cut(s) 1155, 1194, 1296
EcoRII CCWGG 8 cut(s) 460, 532, 669, 871, 1075, 1268, 1360, 1479
EcoT14I CCWWGG 3 cut(s) 171, 282, 860
EcoT22I ATGCAT 1 cut(s) 979
EcoT38I GRGCYC 1 cut(s) 1296
EgeI GGCGCC 1 cut(s) 237
EheI GGCGCC 1 cut(s) 237
ErhI CCWWGG 3 cut(s) 171, 282, 860
FaeI CATG 4 cut(s) 165, 175, 286, 620
FatI CATG 4 cut(s) 161, 171, 282, 616
FbaI TGATCA 1 cut(s) 889
Fnu4HI GCNGC 6 cut(s) 68, 154, 267, 639, 987, 990
FokI GGATG 1 cut(s) 1411
FriOI GRGCYC 1 cut(s) 1296
Fsp4HI GCNGC 6 cut(s) 68, 154, 267, 639, 987, 990
FspBI CTAG 3 cut(s) 95, 527, 738
GlaI GCGC 3 cut(s) 198, 237, 256
GluI GCNGC 6 cut(s) 68, 154, 267, 639, 987, 990
GsaI CCCAGC 1 cut(s) 197
GsuI CTGGAG 3 cut(s) 516, 540, 981
HaeII RGCGCY 3 cut(s) 200, 239, 258
HaeIII GGCC 5 cut(s) 124, 287, 332, 363, 940
HapII CCGG 4 cut(s) 132, 329, 916, 936
HgaI GACGC 2 cut(s) 425, 644
HhaI GCGC 3 cut(s) 199, 238, 257
Hin1I GRCGYC 2 cut(s) 236, 417
Hin1II CATG 4 cut(s) 165, 175, 286, 620
Hin6I GCGC 3 cut(s) 197, 236, 255
HinP1I GCGC 3 cut(s) 197, 236, 255
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HindIII AAGCTT 1 cut(s) 1220
HinfI GANTC 4 cut(s) 515, 1042, 1153, 1185
HpaII CCGG 4 cut(s) 132, 329, 916, 936
HphI GGTGA 1 cut(s) 1369
Hpy166II GTNNAC 2 cut(s) 730, 1119
Hpy188I TCNGA 7 cut(s) 102, 415, 657, 754, 1212, 1278, 1410
Hpy188III TCNNGA 2 cut(s) 519, 1063
Hpy8I GTNNAC 2 cut(s) 730, 1119
HpyAV CCTTC 4 cut(s) 35, 1130, 1137, 1169
HpyCH4III ACNGT 5 cut(s) 589, 682, 852, 1009, 1350
HpyCH4V TGCA 4 cut(s) 67, 269, 609, 977
HpyF10VI GCNNNNNNNGC 7 cut(s) 233, 263, 405, 590, 674, 755, 983
HpyF3I CTNAG 6 cut(s) 274, 549, 654, 942, 1409, 1428
Hsp92I GRCGYC 2 cut(s) 236, 417
Hsp92II CATG 4 cut(s) 165, 175, 286, 620
HspAI GCGC 3 cut(s) 197, 236, 255
KasI GGCGCC 1 cut(s) 235
KpnI GGTACC 1 cut(s) 936
Ksp22I TGATCA 1 cut(s) 889
Kzo9I GATC 4 cut(s) 61, 97, 718, 889
LguI GCTCTTC 1 cut(s) 1301
LmnI GCTCC 6 cut(s) 371, 535, 598, 674, 952, 1331
Lsp1109I GCAGC 4 cut(s) 79, 253, 625, 1001
LweI GCATC 4 cut(s) 105, 408, 758, 964
MaeI CTAG 3 cut(s) 95, 527, 738
MaeIII GTNAC 4 cut(s) 277, 544, 1364, 1375
MalI GATC 4 cut(s) 63, 99, 720, 891
MboI GATC 4 cut(s) 61, 97, 718, 889
MboII GAAGA 7 cut(s) 35, 331, 515, 854, 1288, 1294, 1302
MfeI CAATTG 2 cut(s) 375, 1353
MflI RGATCY 2 cut(s) 61, 97
MhlI GDGCHC 2 cut(s) 922, 1296
MlsI TGGCCA 1 cut(s) 124
MluNI TGGCCA 1 cut(s) 124
Mly113I GGCGCC 1 cut(s) 236
MmeI TCCRAC 4 cut(s) 125, 349, 1309, 1468
Mox20I TGGCCA 1 cut(s) 124
Mph1103I ATGCAT 1 cut(s) 979
MscI TGGCCA 1 cut(s) 124
MseI TTAA 3 cut(s) 773, 822, 1092
MslI CAYNNNNRTG 1 cut(s) 837
Msp20I TGGCCA 1 cut(s) 124
MspI CCGG 4 cut(s) 132, 329, 916, 936
MunI CAATTG 2 cut(s) 375, 1353
MvaI CCWGG 8 cut(s) 462, 534, 671, 873, 1077, 1270, 1362, 1481
MwoI GCNNNNNNNGC 7 cut(s) 233, 263, 405, 590, 674, 755, 983
NarI GGCGCC 1 cut(s) 236
NciI CCSGG 2 cut(s) 917, 937
NcoI CCATGG 2 cut(s) 171, 282
NdeII GATC 4 cut(s) 61, 97, 718, 889
NlaIII CATG 4 cut(s) 165, 175, 286, 620
NlaIV GGNNCC 3 cut(s) 237, 676, 934
NmuCI GTSAC 4 cut(s) 277, 544, 1364, 1375
NsiI ATGCAT 1 cut(s) 979
NspV TTCGAA 1 cut(s) 220
PasI CCCWGGG 1 cut(s) 670
PciSI GCTCTTC 1 cut(s) 1301
PfeI GAWTC 4 cut(s) 515, 1042, 1153, 1185
PkrI GCNGC 6 cut(s) 69, 155, 268, 640, 988, 991
PluTI GGCGCC 1 cut(s) 239
Psp6I CCWGG 8 cut(s) 460, 532, 669, 871, 1075, 1268, 1360, 1479
PspFI CCCAGC 1 cut(s) 193
PspGI CCWGG 8 cut(s) 460, 532, 669, 871, 1075, 1268, 1360, 1479
PspN4I GGNNCC 3 cut(s) 237, 676, 934
PspPI GGNCC 3 cut(s) 134, 938, 1358
PsrI GAACNNNNNNTAC 2 cut(s) 988, 1020
PstI CTGCAG 1 cut(s) 69
PsuI RGATCY 2 cut(s) 61, 97
RsaI GTAC 3 cut(s) 934, 1006, 1118
RsaNI GTAC 3 cut(s) 933, 1005, 1117
RseI CAYNNNNRTG 1 cut(s) 837
SapI GCTCTTC 1 cut(s) 1301
SaqAI TTAA 3 cut(s) 773, 822, 1092
SatI GCNGC 6 cut(s) 68, 154, 267, 639, 987, 990
Sau3AI GATC 4 cut(s) 61, 97, 718, 889
Sau96I GGNCC 3 cut(s) 134, 938, 1358
SduI GDGCHC 2 cut(s) 922, 1296
SfaNI GCATC 4 cut(s) 105, 408, 758, 964
SfcI CTRYAG 4 cut(s) 65, 297, 585, 1031
SfoI GGCGCC 1 cut(s) 237
SfuI TTCGAA 1 cut(s) 220
SinI GGWCC 2 cut(s) 134, 1358
SmiMI CAYNNNNRTG 1 cut(s) 837
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
SsiI CCGC 5 cut(s) 154, 207, 225, 986, 1467
SspDI GGCGCC 1 cut(s) 235
SspMI CTAG 3 cut(s) 95, 527, 738
StyI CCWWGG 3 cut(s) 171, 282, 860
TaaI ACNGT 5 cut(s) 589, 682, 852, 1009, 1350
TaqI TCGA 4 cut(s) 21, 220, 1237, 1339
TaqII GACCGA 1 cut(s) 174
TatI WGTACW 1 cut(s) 1116
TauI GCSGC 2 cut(s) 156, 989
TfiI GAWTC 4 cut(s) 515, 1042, 1153, 1185
Tru1I TTAA 3 cut(s) 773, 822, 1092
Tru9I TTAA 3 cut(s) 773, 822, 1092
TscAI CASTG 6 cut(s) 155, 282, 427, 553, 685, 1465
TseFI GTSAC 4 cut(s) 277, 544, 1364, 1375
TseI GCWGC 4 cut(s) 67, 266, 638, 989
Tsp45I GTSAC 4 cut(s) 277, 544, 1364, 1375
TspDTI ATGAA 4 cut(s) 54, 855, 1295, 1334
TspRI CASTG 6 cut(s) 155, 282, 427, 553, 685, 1465
VpaK11BI GGWCC 2 cut(s) 134, 1358
XapI RAATTY 4 cut(s) 432, 1204, 1415, 1442
XspI CTAG 3 cut(s) 95, 527, 738
Zsp2I ATGCAT 1 cut(s) 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.