Rmu_sc0001791.1_g000015

DA1-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001791.1
OG0002660
Physical Location & Seq
Forward (+)
91901 .. 94397
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1251 bp
atgtctcccaacacagtggatgagccgtctagaacccagaaagagaaagaggaattagaccatgcaattgcgctctctctggctgaagattcaaagagacacagaggatatagatggcaggacaaagataacgagctagctaggtcacttcaggatataaatccaccaatatatcctccacatgcccctccagttcctcaacataatccgtatgcagttcctcaacataatacgtatggaactagaatatgtggtggttgtcagcaagatatacgttacgggagggatccttatcataagtcctgttttaaggagctggcccatccaaaatgtgaagtttgtcatcaatttattccaacgaatcaagctggtttgatcgagtatagatgccatccattttggtcacaaaaatactgtccatcacatgaacatgataacacagctcgttgctgtagttgtgaacgcttagagtcttgggatgcaagatatatttcagtgggagatggacggagtctatgctttgaatgtatggaatcagctatcatggatactggtgattgtcagccattgtatcatgccatcagagattattatgaaggaatgaatatgaaattagatcagcaaattcccatgcttctggttgaaagacaagcacttaacgaagctattgtcggggagaagaatggcctccatcacatgcccgagacaaggggtttatgcctttctgaagagcagacagtcaccagtatactcaaaaggccaagaattggagggcatcaacttatcggaatgagaacccaacctcaaaagttgactcgaagatgtgaagttactgccattcttgttctctatagccttccaagattactaacaggtgctattcttgctcatgagttgatgcacggctggttacgtctcaaaggctaccgtaaccttaatcctgaagtagaggaaggtatctgtcaagtgctttcatacatgtggcttgaaacagaagtaatgccaggattcaaaaacatgccatccacgtctacatgtgcagcttcttcttccacttcctcatcctcatcgaaaaagggtggaaagtcgaatgttgaacagaagcttggtgaatttttcatgcatcaaattgcgaatgattcttccccagcatatggtggagggtttagagcagctaatgcagctgtcaataagtatggtttacgtcgtactctggatcacattcgactcactggaaatttcccattgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0007275 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0022622 GO:0032182 GO:0032501 GO:0032502 GO:0043130 GO:0048364 GO:0048731 GO:0048856 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

416

Amino Acids

47.12

Weight (kDa)

7.26

Isoelectric Point (pI)

55.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 200 - 411 7.8e-101 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 767, 1154
AccI GTMKAC 2 cut(s) 748, 1031
AclWI GGATC 3 cut(s) 281, 294, 1224
AcsI RAATTY 3 cut(s) 624, 1112, 1237
AcuI CTGAAG 4 cut(s) 105, 134, 747, 963
AfaI GTAC 1 cut(s) 1210
AfiI CCNNNNNNNGG 3 cut(s) 708, 767, 1154
AflIII ACRYGT 2 cut(s) 978, 1034
AgsI TTSAA 6 cut(s) 93, 524, 644, 989, 1012, 1097
AjiI CACGTC 1 cut(s) 1029
AjnI CCWGG 1 cut(s) 1003
AjuI GAANNNNNNNTTGG 2 cut(s) 1089, 1121
Alw26I GTCTC 4 cut(s) 9, 91, 698, 920
AlwI GGATC 3 cut(s) 281, 294, 1224
Ama87I CYCGRG 1 cut(s) 701
AoxI GGCC 3 cut(s) 318, 685, 758
ApeKI GCWGC 3 cut(s) 1040, 1172, 1181
ApoI RAATTY 3 cut(s) 624, 1112, 1237
ArsI GACNNNNNNTTYG 4 cut(s) 400, 432, 654, 686
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 319
AsuHPI GGTGA 3 cut(s) 566, 733, 1121
AsuNHI GCTAGC 1 cut(s) 136
AvaI CYCGRG 1 cut(s) 701
BamHI GGATCC 1 cut(s) 286
BbvI GCAGC 3 cut(s) 1052, 1184, 1193
BccI CCATC 8 cut(s) 108, 330, 399, 427, 497, 587, 699, 1030
BceAI ACGGC 2 cut(s) 10, 919
BciT130I CCWGG 1 cut(s) 1005
BciVI GTATCC 1 cut(s) 541
BcoDI GTCTC 4 cut(s) 9, 91, 698, 920
BfaI CTAG 4 cut(s) 30, 137, 141, 243
BfmI CTRYAG 2 cut(s) 451, 850
BfuI GTATCC 1 cut(s) 541
BisI GCNGC 3 cut(s) 1041, 1173, 1182
BlsI GCNGC 3 cut(s) 1042, 1174, 1183
Bme1390I CCNGG 1 cut(s) 1005
BmeT110I CYCGRG 1 cut(s) 701
BmgBI CACGTC 1 cut(s) 1029
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 1 cut(s) 288
BmrFI CCNGG 1 cut(s) 1005
BmsI GCATC 5 cut(s) 377, 469, 784, 888, 1132
BmtI GCTAGC 1 cut(s) 140
BpmI CTGGAG 1 cut(s) 174
BsaAI YACGTR 1 cut(s) 234
BsaBI GATNNNNATC 2 cut(s) 159, 291
Bsc4I CCNNNNNNNGG 3 cut(s) 708, 767, 1154
Bse1I ACTGG 4 cut(s) 191, 556, 744, 1237
Bse8I GATNNNNATC 2 cut(s) 159, 291
BseBI CCWGG 1 cut(s) 1005
BseGI GGATG 6 cut(s) 25, 322, 391, 484, 1022, 1061
BseJI GATNNNNATC 2 cut(s) 159, 291
BseLI CCNNNNNNNGG 3 cut(s) 708, 767, 1154
BseNI ACTGG 4 cut(s) 191, 556, 744, 1237
BseXI GCAGC 3 cut(s) 1052, 1184, 1193
BseYI CCCAGC 1 cut(s) 1147
BsgI GTGCAG 1 cut(s) 1059
BshFI GGCC 3 cut(s) 320, 687, 760
BsiHKCI CYCGRG 1 cut(s) 701
BslI CCNNNNNNNGG 3 cut(s) 708, 767, 1154
BsmAI GTCTC 4 cut(s) 9, 91, 698, 920
BsmBI CGTCTC 1 cut(s) 920
BsnI GGCC 3 cut(s) 320, 687, 760
BsoBI CYCGRG 1 cut(s) 701
Bsp143I GATC 4 cut(s) 286, 375, 616, 1216
BspANI GGCC 3 cut(s) 320, 687, 760
BspHI TCATGA 1 cut(s) 889
BspLI GGNNCC 1 cut(s) 288
BspOI GCTAGC 1 cut(s) 140
BspPI GGATC 3 cut(s) 281, 294, 1224
BspQI GCTCTTC 1 cut(s) 723
BsrI ACTGG 4 cut(s) 191, 556, 744, 1237
BssMI GATC 4 cut(s) 286, 375, 616, 1216
BssNAI GTATAC 1 cut(s) 749
Bst1107I GTATAC 1 cut(s) 749
Bst2UI CCWGG 1 cut(s) 1005
Bst4CI ACNGT 4 cut(s) 16, 416, 739, 929
Bst6I CTCTTC 1 cut(s) 723
BstAPI GCANNNNNTGC 1 cut(s) 1178
BstBAI YACGTR 1 cut(s) 234
BstC8I GCNNGC 2 cut(s) 138, 318
BstDEI CTNAG 1 cut(s) 466
BstF5I GGATG 6 cut(s) 25, 322, 391, 484, 1022, 1061
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 4 cut(s) 289, 378, 619, 1219
BstMAI GTCTC 4 cut(s) 9, 91, 698, 920
BstMBI GATC 4 cut(s) 286, 375, 616, 1216
BstMWI GCNNNNNNNGC 3 cut(s) 884, 1178, 1181
BstNI CCWGG 1 cut(s) 1005
BstNSI RCATGY 5 cut(s) 185, 700, 982, 1021, 1038
BstSCI CCNGG 1 cut(s) 1003
BstSFI CTRYAG 2 cut(s) 451, 850
BstSNI TACGTA 1 cut(s) 234
BstV1I GCAGC 3 cut(s) 1052, 1184, 1193
BstX2I RGATCY 1 cut(s) 286
BstXI CCANNNNNNTGG 2 cut(s) 16, 637
BstYI RGATCY 1 cut(s) 286
BstZ17I GTATAC 1 cut(s) 749
BsuI GTATCC 1 cut(s) 541
BsuRI GGCC 3 cut(s) 320, 687, 760
BtrI CACGTC 1 cut(s) 1029
BtsCI GGATG 6 cut(s) 25, 322, 391, 484, 1022, 1061
BtsIMutI CAGTG 3 cut(s) 21, 501, 1230
Cac8I GCNNGC 2 cut(s) 138, 318
CciI TCATGA 1 cut(s) 889
CfoI GCGC 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 319
Csp6I GTAC 1 cut(s) 1209
CviQI GTAC 1 cut(s) 1209
DdeI CTNAG 1 cut(s) 466
DpnI GATC 4 cut(s) 288, 377, 618, 1218
DpnII GATC 4 cut(s) 286, 375, 616, 1216
Eam1104I CTCTTC 1 cut(s) 723
EarI CTCTTC 1 cut(s) 723
Eco105I TACGTA 1 cut(s) 234
Eco57I CTGAAG 4 cut(s) 105, 134, 747, 963
Eco88I CYCGRG 1 cut(s) 701
EcoRII CCWGG 1 cut(s) 1003
EcoT22I ATGCAT 1 cut(s) 1125
Esp3I CGTCTC 1 cut(s) 920
FauNDI CATATG 1 cut(s) 1153
FblI GTMKAC 2 cut(s) 748, 1031
Fnu4HI GCNGC 3 cut(s) 1041, 1173, 1182
FokI GGATG 6 cut(s) 32, 309, 378, 491, 1009, 1048
Fsp4HI GCNGC 3 cut(s) 1041, 1173, 1182
FspBI CTAG 4 cut(s) 30, 137, 141, 243
GlaI GCGC 1 cut(s) 72
GluI GCNGC 3 cut(s) 1041, 1173, 1182
GsaI CCCAGC 1 cut(s) 1151
GsuI CTGGAG 1 cut(s) 174
HaeIII GGCC 3 cut(s) 320, 687, 760
HhaI GCGC 1 cut(s) 73
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HincII GTYRAC 1 cut(s) 813
HindII GTYRAC 1 cut(s) 813
HindIII AAGCTT 1 cut(s) 1103
HinfI GANTC 9 cut(s) 89, 361, 470, 511, 533, 814, 1008, 1139, 1227
HphI GGTGA 3 cut(s) 566, 733, 1121
Hpy166II GTNNAC 5 cut(s) 461, 749, 813, 1032, 1202
Hpy188I TCNGA 3 cut(s) 584, 727, 788
Hpy188III TCNNGA 5 cut(s) 30, 152, 890, 941, 1214
Hpy8I GTNNAC 5 cut(s) 461, 749, 813, 1032, 1202
Hpy99I CGWCG 1 cut(s) 1209
HpyAV CCTTC 3 cut(s) 590, 866, 947
HpyCH4III ACNGT 4 cut(s) 16, 416, 739, 929
HpyCH4IV ACGT 5 cut(s) 233, 274, 913, 1028, 1204
HpyCH4V TGCA 7 cut(s) 65, 215, 482, 901, 1040, 1123, 1181
HpyF10VI GCNNNNNNNGC 3 cut(s) 884, 1178, 1181
HpyF3I CTNAG 1 cut(s) 466
HpySE526I ACGT 5 cut(s) 233, 274, 913, 1028, 1204
HspAI GCGC 1 cut(s) 71
Kzo9I GATC 4 cut(s) 286, 375, 616, 1216
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 1 cut(s) 313
Lsp1109I GCAGC 3 cut(s) 1052, 1184, 1193
LweI GCATC 5 cut(s) 377, 469, 784, 888, 1132
MaeI CTAG 4 cut(s) 30, 137, 141, 243
MaeII ACGT 5 cut(s) 233, 274, 913, 1028, 1204
MaeIII GTNAC 7 cut(s) 144, 275, 402, 739, 829, 909, 929
MalI GATC 4 cut(s) 288, 377, 618, 1218
MboI GATC 4 cut(s) 286, 375, 616, 1216
MboII GAAGA 7 cut(s) 98, 691, 740, 831, 1038, 1041, 1134
MfeI CAATTG 1 cut(s) 66
MflI RGATCY 1 cut(s) 286
MluCI AATT 9 cut(s) 53, 66, 347, 611, 624, 765, 1112, 1128, 1237
MlyI GAGTC 4 cut(s) 479, 520, 808, 1221
MmeI TCCRAC 1 cut(s) 380
Mph1103I ATGCAT 1 cut(s) 1125
MseI TTAA 3 cut(s) 309, 657, 936
MslI CAYNNNNRTG 2 cut(s) 429, 979
MspA1I CMGCKG 1 cut(s) 1184
MspR9I CCNGG 1 cut(s) 1005
MunI CAATTG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 1005
MwoI GCNNNNNNNGC 3 cut(s) 884, 1178, 1181
NdeI CATATG 1 cut(s) 1153
NdeII GATC 4 cut(s) 286, 375, 616, 1216
NheI GCTAGC 1 cut(s) 136
NlaIV GGNNCC 1 cut(s) 288
NmuCI GTSAC 3 cut(s) 144, 402, 739
NsiI ATGCAT 1 cut(s) 1125
NspI RCATGY 5 cut(s) 185, 700, 982, 1021, 1038
PagI TCATGA 1 cut(s) 889
PciI ACATGT 2 cut(s) 978, 1034
PciSI GCTCTTC 1 cut(s) 723
PfeI GAWTC 5 cut(s) 89, 361, 533, 1008, 1139
PflFI GACNNNGTC 1 cut(s) 510
PflMI CCANNNNNTGG 2 cut(s) 767, 1154
PkrI GCNGC 3 cut(s) 1042, 1174, 1183
PleI GAGTC 4 cut(s) 478, 519, 808, 1221
PpsI GAGTC 4 cut(s) 478, 519, 808, 1221
Ppu21I YACGTR 1 cut(s) 234
PscI ACATGT 2 cut(s) 978, 1034
Psp6I CCWGG 1 cut(s) 1003
PspFI CCCAGC 1 cut(s) 1147
PspGI CCWGG 1 cut(s) 1003
PspN4I GGNNCC 1 cut(s) 288
PspPI GGNCC 1 cut(s) 319
PsuI RGATCY 1 cut(s) 286
PsyI GACNNNGTC 1 cut(s) 510
PvuII CAGCTG 1 cut(s) 1184
RsaI GTAC 1 cut(s) 1210
RsaNI GTAC 1 cut(s) 1209
RseI CAYNNNNRTG 2 cut(s) 429, 979
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 3 cut(s) 309, 657, 936
SatI GCNGC 3 cut(s) 1041, 1173, 1182
Sau3AI GATC 4 cut(s) 286, 375, 616, 1216
Sau96I GGNCC 1 cut(s) 319
SchI GAGTC 4 cut(s) 479, 520, 808, 1221
ScrFI CCNGG 1 cut(s) 1005
SfaNI GCATC 5 cut(s) 377, 469, 784, 888, 1132
SfcI CTRYAG 2 cut(s) 451, 850
SmiMI CAYNNNNRTG 2 cut(s) 429, 979
SnaBI TACGTA 1 cut(s) 234
Sse9I AATT 9 cut(s) 53, 66, 347, 611, 624, 765, 1112, 1128, 1237
SspMI CTAG 4 cut(s) 30, 137, 141, 243
StyD4I CCNGG 1 cut(s) 1003
TaaI ACNGT 4 cut(s) 16, 416, 739, 929
TaiI ACGT 5 cut(s) 236, 277, 916, 1031, 1207
TaqI TCGA 5 cut(s) 378, 817, 1070, 1088, 1225
TasI AATT 9 cut(s) 53, 66, 347, 611, 624, 765, 1112, 1128, 1237
TfiI GAWTC 5 cut(s) 89, 361, 533, 1008, 1139
Tru1I TTAA 3 cut(s) 309, 657, 936
Tru9I TTAA 3 cut(s) 309, 657, 936
TscAI CASTG 3 cut(s) 21, 501, 1237
TseFI GTSAC 3 cut(s) 144, 402, 739
TseI GCWGC 3 cut(s) 1040, 1172, 1181
Tsp45I GTSAC 3 cut(s) 144, 402, 739
TspDTI ATGAA 6 cut(s) 441, 609, 617, 623, 963, 1108
TspGWI ACGGA 2 cut(s) 198, 523
TspRI CASTG 3 cut(s) 21, 501, 1237
Tth111I GACNNNGTC 1 cut(s) 510
Van91I CCANNNNNTGG 2 cut(s) 767, 1154
XapI RAATTY 3 cut(s) 624, 1112, 1237
XbaI TCTAGA 1 cut(s) 29
XceI RCATGY 5 cut(s) 185, 700, 982, 1021, 1038
XmiI GTMKAC 2 cut(s) 748, 1031
XspI CTAG 4 cut(s) 30, 137, 141, 243
Zsp2I ATGCAT 1 cut(s) 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.