Rmu_sc0001809.1_g000026

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001809.1
OG0013097
Physical Location & Seq
Forward (+)
102493 .. 115802
13310 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 6438 bp
atggcgtctggttcgcctcctccgtcgcgcatcaatcagttttttgcttcgaagaaacggaagcctgtatcacctgctttgaagtcggggagaaatgaaaaggatgtacaaactgtagaaggttcacctagtgctaaggccacattggacagttatttgcggacatcgccggagagttataatgtggcgaaacctttgtgcaaatttggtgattcagcggctcggcaagatcaagttagaaggaatttggcatctgagatggataattcttcaaaaaatgaatgtatgcagcaccgtttctcttctcaactgcacactcatgcaagtgaggcttctggagcaaatcagaaggaaatagctaagggattaaccgaggcgggtgatgttgtagctggaggttctttcaaggattactcagattttgcaggaggtgtggagaatgtagagctgaagaagtttgcagccaactttttgtctttgtattgcagtgatgttcaatcaaacaaaagttctgtgtcagagctgaaagtgaatggtcataagagataccctagcccatctttgcttgatggggagagtaagacatttaaaaagaggcattgcggcagtgataaatcctatgaagagcgtgaaacaattcgttctactgagaagagctatgaagcaatgcaatctgcttgtgttgataaaaatagggttaccattcctaatgaattactcgagcttcaacaaaccttgaaaacatgcagtaatacacccaaactgtctctagacgtagttgattgttatacaccaggctcattgattaaatcatgtgttcgtcagacccccaattcaatacgtggtagctcattttcttcacctggtgaagctttttggaacgatgcaattcaacttgctgatggtctatgtgcccaggcagcagaagcaactacagcttcagatagccaatatcatggtataaactcttgcaatttgagaaacgcacactgtgatgaaaagtcaaaggaaatgatggatgaaggtgagcgtatgggtaatggtgctgtatcagagccaatggtaaagcataggaaggacttgcataaagaagtatccccactgcctgttaagcattttgatttctctcttgaggacaagatttcgaatgaaagtatgccacatcatatagattcttacaatttgaaaagcatggctcatggaggtggcgagcaatctgaatccagtcttataggtcctagaggtctaagccatggtattactatggtccgttgcagcaaatctcaggcaaatcaagggcaagatgttgattctgttactgcagtgaccaaaatgaaagtggatttgtctagcaaggataatggtggcccgacattttcttcaccaggtgatgatgtcgttaagttgaatggtaaaggtgaatctagcgaagaaagtactcctttaagctttctgccactgaaagattgtctggatctagatggttggcttgcaccagaaatatgcagcatatatagaaagaaaggaatttccaagctttatccttggcaggtcttgtcatctggaaaaatagctttacttgtacttccttatgtatcactttgcgcagaaaaggcagaacatctggagctacttcttgaaccacttggtaagcgtgttcgtagttattatgggaaccaaggtggtggtacacttcctaaagacactactgtagctgtctgcacaatagaaaaggcaaactttttagtaaacaagctgctggaagagggccgtctttcagaaattggaattgttgtgatagatgaactgcacatggttggcgatccaagtaggggttatcttctggaacttctgttgacaaaacttcgttatgcagctggtgaaggcatttctgaatcaagtagtggagaaagttcaggcatgagcagcggtaaggctgaccctgcccatggtttgcaaattgttggtatgagtgccactatgccaaatgtggcagcagttgctgattggcttcaagctgccctttaccagacagaatttcgaccggtaccactagaagaatacattaaagtagggaacaccatttataacaagaaaatggagattattagaacaatcccaaaagcagttgaccttggtggtaaagatccagatcatgttgtggaactgtgcaatgaggttgtacaagaaggtctttccgtgttaatattttgctctagtcgaaaaggatgtgaatcaacagcgaggcatgtttcaagattccttaagaaatttgcagtcaacattcgcactgatgacagtgaatttaatgatgtgacattggctattgattccttacgaaggtgtcctgctggtttggatccagtgttagaagaaaccctaccttctggagttgcctaccaccatgctgggctcacggttgaggaaagagagattgttgaaacctgctaccgtaaaggccatgtacgcatcttaactgcaacatctaccttagcagctggtgttaatctgccagctaggagagtcatttttcgtcagccccggattggtcgcgattttatcgatgggacaaggtacaggcaaatggctggtcgggctggtcgtactggcatagatacaaaaggagaaagtgtactaatttgcaagccagaagagatcaaaaggattatgggagttcttaacgaaagctgtctgccattgcgctcttgtttatctgaagatatgaatggaatgactcatgcaatcttagaagttgtagcagggggaattgttcaaactgctaatgacattcatcgctatgttaggtgcacacttttaaactccactaaaccttttcaggatgtggtgaaatcagcacaggaatctctccgttggttgtgccacaagaaatttctagaatggaatgacgacactaagttatacaataccacaccccttggacgtgcagcttttggcagttctctatgtcctgaagaatcacttattgtgctggatgatctttcaagagcaagagaaggctttgtgcttgcttctgatttgcatctggtttacctagtaacaccaataaatgttgatgttgagcctgattgggaactgtattatgaacagttcatggaattgtctgctcttgaccagtctgtaggcaatcgcgttggagtgacagaaccatttttgatgcgaatggcacatggtgcacctatgcggtcatcaaacaggatgagagaaaatacgaaagcgttgcgtggcaattatgaaagccgacttgggactacaaagagtactgtgcttccggatgatcaaacagttcgagtttgtaaacggttctatgttgctctgatcttgtcaagactagttcaggaagttgctatcaatgaggtctgtgaagcttttaaagtggctagaggtatggtccaggccttacaagagaatgctggaaggtttgcatctatggtaactatgttttgtgaaagactcggatggcatgatctggaaggcttggttggaaaatttcaaaatcgtgtttcatttggagtgagagcagagattgtagagctgactacaattccatttgtcaagggttctcgagcaagggcactctataaagctggcttgcgtacgcctttagctattgctgaagcatccatccctgaaattgtcaaagctctttttgaatcttcatcatgggcagcacaagaaggttcagctcaaagacgcatccaagtaggagtagccaagaagatcaagaagggtgcacacaaaattgttcttgaaaaggcggaagaggccagagttgctgccttctctgcttttaaatcacttggattggaggtcccacagttttctcgaccattatttccaactgctggtggaagtcccactatgcaaggaacaaaaaattcctctggtgatgaaaatactactagctttgttaatgcccatgtggaggatgctcttaagccatctttggaaggaagggttccttctggtaaggttgtatcaggtgtgaagttgacaaaaacatcagatattggtacattggtggcttcaggagaagtaacttcaagtggtgtcatgcaaattaagtttggggctgaaaattctgtagtaccaattgatgggtctggtgcccttgctgatgagctaaatgctactgttcatcaaactaaaattgctgacttgaccaactcgattaaggtagacagcttaggtgatagaaataggatatccagcaagtctttagatcctaaaaagcagggaagctgtgaaggggaaaatttgtctgttggtgttaagggcaatgcttgtgaaaaaggccctattcatgctatttacactcctggtggaatcgattcatttttgaatatttgggaaaccacctcagagtttcattttgatatccactacaacaaaagatcagaattgaactctgttgccccctttgaaattcatggaatggccatctgttgggaagattctcctgtgtactatgttaacattcctaaggatctgctgtggtctggcagcagcagtaaagacaaagggctgcgtttgactgcatcaggtgatagaactaatgtgttacctcttgatggtatgttggagctggctaaacgtagatggagcaggattggtgaaatcatggggaaaagaggtgtcaggaaatttacttggatgttgaaaagtcagattcaggcattgaagtgccctgcagttcaaatacagagatttggttcccagagtcttgcagggaaaagtatttgttttgaaattatagacacctcgtttttattgttgccacccgttcatatacaagatggcattgacatgtgcattgtggcctggattctttggcctgatgaggagaaaaattctaatccaaacttggaaaaggaagttaagaaaaggttatccagtgacgctgcagcagctgcaaatcgaaacggcaggtggaaaaatcagatgcgaagagctgcacacaatggttgctgccgtcgagttgctcaaacacgggccttatattctgttctgtggaagttgcttgtttctgaagaccttgctgagtcacttataaatatagaagttccattggttaatattcttgctgacatggagctttggggagtgggtcttgatatggagggatgtctgcaagcacgtaatgtgctggggaagaagctaagacatcttgagaaagaagcttataaactggctggcatgacattttcattatatacagcagcagatattgctaatgtgctttatggacacttaaagctgcccataccagaggtgcgcaacaagggaaaacaacatccaagtactgacaagcattgcttggatttattaagggacgagcatcctatcattcccatcatcaaagagcatcgtacattggcaaaactcttaaactgtacgttgggatcaatttgctcacttgctaggctatctgtgaagacacagaagtacacattacacggtcattggctccaaacatcaacagctactggtcggctttcaatggaggaacctaatctccagtgtgtggaacatatggttgacttcaaaatgaacaagtatgaaaatggaggcaaaagtgatgttgatcattacaaaattaatgctcgagaatactttatacctactcaggacaactggatactgttgaccgcagattattctcagatagaactgcggctgatggcccatttttctgaggattcctcactcattgaactccttagtaagccccatggcgatgtcttcaccatgattgcagctagatggactggaatatcagaagattgtattagttctgatgtacgagatcagaccaaaaggttggtgtatggaattctctacggaatgggtgctaatagccttgcagaacaactggattgcagtccccaggcagcttctgagaaaatacaaaacttcaaaagttctttccccggagttgcttcttggctcaatgaagcagttggatgttgccgcaagaaaggatatgtggaaacccttaagggaagaaaacgatttttgtcaaaaataaaatttgggaacagcaaggaaaagtcaaaggcgcagagacaggctgtaaattcgatatgtcagggatcagctgctgacataatcaagattgcaatgataaatatttactccgtaatagttgatggagctgaaaagcctgatttgacttctccatttgcaacaaagtttcacaaactgaagggccgctgccgaattctattgcaggtacatgatgaattggtacttgaagttgatccttctgtgataaaggaagctgctttgctgttacaaacaagcatggaaagtgctgtttcgcttcttgttcctatgcatgtcaaattgaaagttggaagaacttggggttctttgcaaccctttcaggctgaccaatatgaagatctgcctgatccataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000724 GO:0000725 GO:0000726 GO:0002200 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002566 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003684 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006282 GO:0006284 GO:0006302 GO:0006303 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008094 GO:0008150 GO:0008152 GO:0008409 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009933 GO:0009987 GO:0010468 GO:0010564 GO:0010569 GO:0010605 GO:0016043 GO:0016445 GO:0016446 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016829 GO:0016835 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022607 GO:0031323 GO:0031324 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042623 GO:0042802 GO:0043142 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045738 GO:0045910 GO:0045934 GO:0046483 GO:0048507 GO:0048519 GO:0048523 GO:0048532 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051259 GO:0051260 GO:0051301 GO:0051575 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0097159 GO:0097681 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901987 GO:1902749 GO:1990067 GO:2000011 GO:2000026 GO:2000042 GO:2000779 GO:2000780 GO:2001020 GO:2001021
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DNA_pol_A Helicase_C

Protein Analysis

2145

Amino Acids

236.24

Weight (kDa)

8.07

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 520 - 665 2.6e-10 DEAD/DEAH box helicase
Helicase_C PF00271 795 - 870 1.5e-09 Helicase conserved C-terminal domain
HTH_61 PF20470 875 - 966 9.5e-25 Helix-turn-helix domain
POLQ_helical PF21099 990 - 1174 9.6e-53 DNA_pol_Q helicase like region helical domain
DUF7898 PF25453 1180 - 1254 5.9e-07 Domain of unknown function (DUF7898)
DNA_pol_A PF00476 1717 - 2129 5.3e-110 DNA polymerase family A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32700
fragaria_vesca FvH4_2g36240 FvH4_2g36240
malus_domestica MD08G1120000.v1.1
prunus_persica Prupe.1G453900_v2.0.a1 Prupe.1G453900_v2.0.a1
pyrus_communis pycom15g09100
rosa_chinensis RchiOBHm_Chr6g0305641
rosa_laevigata RLG00000010870
rosa_multiflora Rmu_sc0001809.1_g000026
rosa_roxburghii Rroxscaffold_7G00162490
rosa_rugosa Rorug06G0342400 Rorug06G0342500
rosa_samantha Rh6AG454300 Rh6BG447200 Rh6CG468000 Rh6DG454800
rosa_wichuraiana Rw6G039610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 180, 2088, 5069, 5202
AarI CACCTGC 2 cut(s) 82, 4935
Acc16I TGCGCA 2 cut(s) 1606, 5294
Acc36I ACCTGC 5 cut(s) 82, 1540, 2453, 4935, 6238
Acc65I GGTACC 1 cut(s) 2047
AccB1I GGYRCC 2 cut(s) 2047, 4124
AccB7I CCANNNNNTGG 4 cut(s) 3863, 4115, 4464, 5542
AccI GTMKAC 1 cut(s) 4197
AccII CGCG 3 cut(s) 28, 2553, 3150
AccIII TCCGGA 1 cut(s) 3289
AcoI YGGCCR 1 cut(s) 4455
AcuI CTGAAG 8 cut(s) 470, 924, 2736, 2991, 3654, 4029, 5067, 6242
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 5 cut(s) 131, 866, 992, 1388, 3191
AflII CTTAAG 3 cut(s) 2264, 3953, 6014
AflIII ACRYGT 1 cut(s) 4824
AgeI ACCGGT 1 cut(s) 2044
AhlI ACTAGT 1 cut(s) 3349
AjiI CACGTC 1 cut(s) 2942
AjnI CCWGG 8 cut(s) 793, 862, 915, 1384, 3411, 4336, 4838, 5904
AjuI GAANNNNNNNTTGG 4 cut(s) 3246, 3278, 3483, 3515
AloI GAACNNNNNNTCC 2 cut(s) 5517, 5549
Alw21I GWGCWC 3 cut(s) 2810, 3196, 3754
Alw26I GTCTC 2 cut(s) 771, 6076
Alw44I GTGCAC 3 cut(s) 2806, 3192, 3750
AlwNI CAGNNNCTG 5 cut(s) 2003, 4928, 5504, 5915, 6119
Ama87I CYCGRG 3 cut(s) 719, 3582, 5622
Aor13HI TCCGGA 1 cut(s) 3289
ApaLI GTGCAC 3 cut(s) 2806, 3192, 3750
ArsI GACNNNNNNTTYG 2 cut(s) 2575, 2607
AseI ATTAAT 1 cut(s) 5616
AsiGI ACCGGT 1 cut(s) 2044
Asp700I GAANNNNTTC 2 cut(s) 636, 4348
Asp718I GGTACC 1 cut(s) 2047
AspLEI GCGC 5 cut(s) 30, 1607, 2703, 5295, 6079
AsuC2I CCSGG 2 cut(s) 2542, 5949
AsuII TTCGAA 2 cut(s) 50, 1145
AvaI CYCGRG 3 cut(s) 719, 3582, 5622
AvaII GGWCC 4 cut(s) 1235, 1267, 3409, 3829
AxyI CCTNAGG 1 cut(s) 4500
BaeGI GKGCMC 7 cut(s) 916, 2810, 3196, 3595, 3754, 4129, 4706
BaeI ACNNNNGTAYC 2 cut(s) 4023, 4056
BalI TGGCCA 1 cut(s) 4457
BamHI GGATCC 1 cut(s) 2359
BanI GGYRCC 2 cut(s) 2047, 4124
BanII GRGCYC 1 cut(s) 2415
BarI GAAGNNNNNNTAC 2 cut(s) 2364, 2396
BbsI GAAGAC 3 cut(s) 5055, 5459, 5752
Bbv12I GWGCWC 3 cut(s) 2810, 3196, 3754
BceAI ACGGC 3 cut(s) 1755, 4957, 4974
BcgI CGANNNNNNTGC 2 cut(s) 3220, 3254
BciT130I CCWGG 8 cut(s) 795, 864, 917, 1386, 3413, 4338, 4840, 5906
BciVI GTATCC 2 cut(s) 1105, 5649
BclI TGATCA 2 cut(s) 3295, 5602
BcnI CCSGG 2 cut(s) 2542, 5949
BcoDI GTCTC 2 cut(s) 771, 6076
BcuI ACTAGT 1 cut(s) 3349
BfmI CTRYAG 8 cut(s) 114, 933, 1320, 1710, 3138, 4101, 4707, 4920
BfrI CTTAAG 3 cut(s) 2264, 3953, 6014
BfuAI ACCTGC 5 cut(s) 82, 1540, 2453, 4935, 6238
BfuI GTATCC 2 cut(s) 1105, 5649
BglII AGATCT 1 cut(s) 6421
BmcAI AGTACT 3 cut(s) 1438, 3280, 5320
Bme18I GGWCC 4 cut(s) 1235, 1267, 3409, 3829
BmeT110I CYCGRG 3 cut(s) 719, 3582, 5622
BmgBI CACGTC 1 cut(s) 2942
BmiI GGNNCC 9 cut(s) 1676, 2049, 2361, 3831, 3978, 4126, 4732, 5486, 5526
BpiI GAAGAC 3 cut(s) 5055, 5459, 5752
BplI GAGNNNNNCTC 2 cut(s) 5705, 5737
BpmI CTGGAG 5 cut(s) 357, 414, 1646, 2409, 5519
Bpu10I CCTNAGC 4 cut(s) 135, 360, 2491, 4204
Bpu14I TTCGAA 2 cut(s) 50, 1145
BpuEI CTTGAG 2 cut(s) 1151, 5207
BpuMI CCSGG 2 cut(s) 2542, 5949
Bsa29I ATCGAT 2 cut(s) 2562, 4347
BsaAI YACGTR 2 cut(s) 842, 5156
BsaBI GATNNNNATC 4 cut(s) 2151, 2233, 3039, 5601
BsaHI GRCGYC 1 cut(s) 5
BsaWI WCCGGW 2 cut(s) 2044, 3289
BsaXI ACNNNNNCTCC 4 cut(s) 2616, 2646, 5517, 5547
Bse118I RCCGGY 1 cut(s) 2044
Bse21I CCTNAGG 1 cut(s) 4500
Bse3DI GCAATG 7 cut(s) 598, 672, 2179, 2696, 4303, 5329, 6144
Bse8I GATNNNNATC 4 cut(s) 2151, 2233, 3039, 5601
BseAI TCCGGA 1 cut(s) 3289
BseBI CCWGG 8 cut(s) 795, 864, 917, 1386, 3413, 4338, 4840, 5906
BseCI ATCGAT 2 cut(s) 2562, 4347
BseJI GATNNNNATC 4 cut(s) 2151, 2233, 3039, 5601
BseMI GCAATG 7 cut(s) 598, 672, 2179, 2696, 4303, 5329, 6144
BseRI GAGGAG 2 cut(s) 9, 4874
BseSI GKGCMC 7 cut(s) 916, 2810, 3196, 3595, 3754, 4129, 4706
BseYI CCCAGC 2 cut(s) 2408, 5164
BsgI GTGCAG 5 cut(s) 296, 1705, 1793, 2964, 4956
Bsh1236I CGCG 3 cut(s) 28, 2553, 3150
Bsh1285I CGRYCG 1 cut(s) 2045
BshNI GGYRCC 2 cut(s) 2047, 4124
BshTI ACCGGT 1 cut(s) 2044
BshVI ATCGAT 2 cut(s) 2562, 4347
BsiEI CGRYCG 1 cut(s) 2045
BsiHKAI GWGCWC 3 cut(s) 2810, 3196, 3754
BsiHKCI CYCGRG 3 cut(s) 719, 3582, 5622
BsiSI CCGG 5 cut(s) 170, 2045, 2542, 3290, 5949
BsiWI CGTACG 1 cut(s) 3614
BslFI GGGAC 6 cut(s) 2581, 3280, 3815, 3858, 5363, 5886
BsmAI GTCTC 2 cut(s) 771, 6076
BsmFI GGGAC 6 cut(s) 2581, 3280, 3815, 3858, 5363, 5886
BsmI GAATGC 1 cut(s) 3433
BsoBI CYCGRG 3 cut(s) 719, 3582, 5622
Bsp119I TTCGAA 2 cut(s) 50, 1145
Bsp1286I GDGCHC 8 cut(s) 916, 2415, 2810, 3196, 3595, 3754, 4129, 4706
Bsp13I TCCGGA 1 cut(s) 3289
Bsp1407I TGTACA 2 cut(s) 106, 2182
Bsp19I CCATGG 3 cut(s) 1252, 1948, 5749
Bsp68I TCGCGA 1 cut(s) 2553
BspDI ATCGAT 2 cut(s) 2562, 4347
BspEI TCCGGA 1 cut(s) 3289
BspFNI CGCG 3 cut(s) 28, 2553, 3150
BspLI GGNNCC 9 cut(s) 1676, 2049, 2361, 3831, 3978, 4126, 4732, 5486, 5526
BspMAI CTGCAG 3 cut(s) 1324, 4711, 4924
BspMI ACCTGC 5 cut(s) 82, 1540, 2453, 4935, 6238
BspQI GCTCTTC 3 cut(s) 618, 647, 4960
BspT104I TTCGAA 2 cut(s) 50, 1145
BspT107I GGYRCC 2 cut(s) 2047, 4124
BspTI CTTAAG 3 cut(s) 2264, 3953, 6014
BsrDI GCAATG 7 cut(s) 598, 672, 2179, 2696, 4303, 5329, 6144
BsrFI RCCGGY 1 cut(s) 2044
BsrGI TGTACA 2 cut(s) 106, 2182
BssAI RCCGGY 1 cut(s) 2044
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 7 cut(s) 1252, 1544, 1678, 1948, 2134, 2935, 5749
Bst2UI CCWGG 8 cut(s) 795, 864, 917, 1386, 3413, 4338, 4840, 5906
Bst6I CTCTTC 7 cut(s) 307, 618, 647, 1758, 2646, 3774, 4960
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 3 cut(s) 2264, 3953, 6014
BstAPI GCANNNNNTGC 4 cut(s) 2000, 3191, 4928, 5246
BstAUI TGTACA 2 cut(s) 106, 2182
BstBAI YACGTR 2 cut(s) 842, 5156
BstBI TTCGAA 2 cut(s) 50, 1145
BstDSI CCRYGG 3 cut(s) 1252, 1948, 5749
BstEII GGTNACC 1 cut(s) 697
BstENI CCTNNNNNAGG 1 cut(s) 2338
BstFNI CGCG 3 cut(s) 28, 2553, 3150
BstHHI GCGC 5 cut(s) 30, 1607, 2703, 5295, 6079
BstMAI GTCTC 2 cut(s) 771, 6076
BstMCI CGRYCG 1 cut(s) 2045
BstNI CCWGG 8 cut(s) 795, 864, 917, 1386, 3413, 4338, 4840, 5906
BstNSI RCATGY 4 cut(s) 747, 2252, 4828, 6359
BstPI GGTNACC 1 cut(s) 697
BstSFI CTRYAG 8 cut(s) 114, 933, 1320, 1710, 3138, 4101, 4707, 4920
BstSLI GKGCMC 7 cut(s) 916, 2810, 3196, 3595, 3754, 4129, 4706
BstUI CGCG 3 cut(s) 28, 2553, 3150
BstV2I GAAGAC 3 cut(s) 5055, 5459, 5752
BstX2I RGATCY 6 cut(s) 1474, 2146, 2359, 4240, 4504, 6421
BstXI CCANNNNNNTGG 4 cut(s) 956, 1685, 2408, 5839
BstYI RGATCY 6 cut(s) 1474, 2146, 2359, 4240, 4504, 6421
Bsu15I ATCGAT 2 cut(s) 2562, 4347
Bsu36I CCTNAGG 1 cut(s) 4500
BsuI GTATCC 2 cut(s) 1105, 5649
BsuTUI ATCGAT 2 cut(s) 2562, 4347
BtgI CCRYGG 3 cut(s) 1252, 1948, 5749
BtgZI GCGATG 3 cut(s) 150, 2777, 5769
BtrI CACGTC 1 cut(s) 2942
BtsI GCAGTG 4 cut(s) 493, 613, 1100, 1329
BtuMI TCGCGA 1 cut(s) 2553
BveI ACCTGC 5 cut(s) 82, 1540, 2453, 4935, 6238
CaiI CAGNNNCTG 5 cut(s) 2003, 4928, 5504, 5915, 6119
CfoI GCGC 5 cut(s) 30, 1607, 2703, 5295, 6079
Cfr10I RCCGGY 1 cut(s) 2044
ClaI ATCGAT 2 cut(s) 2562, 4347
CseI GACGC 2 cut(s) 3720, 4925
CsiI ACCWGGT 2 cut(s) 862, 1384
CspAI ACCGGT 1 cut(s) 2044
CspCI CAANNNNNGTGG 2 cut(s) 2917, 2952
DraI TTTAAA 4 cut(s) 589, 2817, 3391, 3811
DraIII CACNNNGTG 5 cut(s) 131, 866, 992, 1388, 3191
EaeI YGGCCR 1 cut(s) 4455
Eam1104I CTCTTC 7 cut(s) 307, 618, 647, 1758, 2646, 3774, 4960
EarI CTCTTC 7 cut(s) 307, 618, 647, 1758, 2646, 3774, 4960
EciI GGCGGA 1 cut(s) 3791
Eco130I CCWWGG 7 cut(s) 1252, 1544, 1678, 1948, 2134, 2935, 5749
Eco147I AGGCCT 1 cut(s) 3416
Eco24I GRGCYC 1 cut(s) 2415
Eco32I GATATC 2 cut(s) 4224, 4396
Eco47I GGWCC 4 cut(s) 1235, 1267, 3409, 3829
Eco57I CTGAAG 8 cut(s) 470, 924, 2736, 2991, 3654, 4029, 5067, 6242
Eco81I CCTNAGG 1 cut(s) 4500
Eco88I CYCGRG 3 cut(s) 719, 3582, 5622
Eco91I GGTNACC 1 cut(s) 697
EcoNI CCTNNNNNAGG 1 cut(s) 2338
EcoO109I RGGNCCY 3 cut(s) 1235, 3829, 4313
EcoO65I GGTNACC 1 cut(s) 697
EcoRI GAATTC 2 cut(s) 5850, 6237
EcoRII CCWGG 8 cut(s) 793, 862, 915, 1384, 3411, 4336, 4838, 5904
EcoRV GATATC 2 cut(s) 4224, 4396
EcoT14I CCWWGG 7 cut(s) 1252, 1544, 1678, 1948, 2134, 2935, 5749
EcoT22I ATGCAT 1 cut(s) 6357
EcoT38I GRGCYC 1 cut(s) 2415
ErhI CCWWGG 7 cut(s) 1252, 1544, 1678, 1948, 2134, 2935, 5749
FaqI GGGAC 6 cut(s) 2581, 3280, 3815, 3858, 5363, 5886
FauI CCCGC 1 cut(s) 370
FauNDI CATATG 1 cut(s) 5550
FbaI TGATCA 2 cut(s) 3295, 5602
FblI GTMKAC 1 cut(s) 4197
FriOI GRGCYC 1 cut(s) 2415
FspI TGCGCA 2 cut(s) 1606, 5294
GlaI GCGC 5 cut(s) 29, 1606, 2702, 5294, 6078
GsaI CCCAGC 2 cut(s) 2412, 5168
GsuI CTGGAG 5 cut(s) 357, 414, 1646, 2409, 5519
HapII CCGG 5 cut(s) 170, 2045, 2542, 3290, 5949
HgaI GACGC 2 cut(s) 3720, 4925
HhaI GCGC 5 cut(s) 30, 1607, 2703, 5295, 6079
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 5 cut(s) 28, 1605, 2701, 5293, 6077
HinP1I GCGC 5 cut(s) 28, 1605, 2701, 5293, 6077
HincII GTYRAC 7 cut(s) 1857, 2131, 2281, 4011, 4492, 5557, 5664
HindII GTYRAC 7 cut(s) 1857, 2131, 2281, 4011, 4492, 5557, 5664
HindIII AAGCTT 5 cut(s) 870, 1447, 1535, 3384, 5196
HpaI GTTAAC 1 cut(s) 4492
HpaII CCGG 5 cut(s) 170, 2045, 2542, 3290, 5949
Hpy99I CGWCG 2 cut(s) 28, 4995
HpyCH4IV ACGT 6 cut(s) 774, 841, 2941, 4612, 5155, 5414
HpySE526I ACGT 6 cut(s) 774, 841, 2941, 4612, 5155, 5414
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 5 cut(s) 28, 1605, 2701, 5293, 6077
Kpn2I TCCGGA 1 cut(s) 3289
KpnI GGTACC 1 cut(s) 2051
Ksp22I TGATCA 2 cut(s) 3295, 5602
KspAI GTTAAC 1 cut(s) 4492
LguI GCTCTTC 3 cut(s) 618, 647, 4960
LmnI GCTCC 7 cut(s) 338, 1627, 4600, 4620, 5110, 5490, 6170
MabI ACCWGGT 2 cut(s) 862, 1384
MaeII ACGT 6 cut(s) 774, 841, 2941, 4612, 5155, 5414
MfeI CAATTG 1 cut(s) 4110
MflI RGATCY 6 cut(s) 1474, 2146, 2359, 4240, 4504, 6421
MhlI GDGCHC 8 cut(s) 916, 2415, 2810, 3196, 3595, 3754, 4129, 4706
MlsI TGGCCA 1 cut(s) 4457
MluNI TGGCCA 1 cut(s) 4457
MlyI GAGTC 5 cut(s) 2532, 2728, 3465, 4747, 5069
MmeI TCCRAC 6 cut(s) 3133, 3481, 3881, 4578, 5959, 6352
Mox20I TGGCCA 1 cut(s) 4457
Mph1103I ATGCAT 1 cut(s) 6357
MroI TCCGGA 1 cut(s) 3289
MroXI GAANNNNTTC 2 cut(s) 636, 4348
MscI TGGCCA 1 cut(s) 4457
MslI CAYNNNNRTG 8 cut(s) 318, 324, 993, 1203, 2796, 4699, 4823, 5754
Msp20I TGGCCA 1 cut(s) 4457
MspA1I CMGCKG 7 cut(s) 218, 1877, 1929, 2498, 4928, 6116, 6231
MspCI CTTAAG 3 cut(s) 2264, 3953, 6014
MspI CCGG 5 cut(s) 170, 2045, 2542, 3290, 5949
MunI CAATTG 1 cut(s) 4110
Mva1269I GAATGC 1 cut(s) 3433
MvaI CCWGG 8 cut(s) 795, 864, 917, 1386, 3413, 4338, 4840, 5906
MvnI CGCG 3 cut(s) 28, 2553, 3150
NciI CCSGG 2 cut(s) 2542, 5949
NcoI CCATGG 3 cut(s) 1252, 1948, 5749
NdeI CATATG 1 cut(s) 5550
NlaIV GGNNCC 9 cut(s) 1676, 2049, 2361, 3831, 3978, 4126, 4732, 5486, 5526
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 5 cut(s) 1324, 2314, 3157, 4913, 5061
NruI TCGCGA 1 cut(s) 2553
NsbI TGCGCA 2 cut(s) 1606, 5294
NsiI ATGCAT 1 cut(s) 6357
NspI RCATGY 4 cut(s) 747, 2252, 4828, 6359
NspV TTCGAA 2 cut(s) 50, 1145
PaeR7I CTCGAG 3 cut(s) 719, 3582, 5622
PaqCI CACCTGC 2 cut(s) 82, 4935
PceI AGGCCT 1 cut(s) 3416
PciI ACATGT 1 cut(s) 4824
PciSI GCTCTTC 3 cut(s) 618, 647, 4960
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 2599
PctI GAATGC 1 cut(s) 3433
PdmI GAANNNNTTC 2 cut(s) 636, 4348
Pfl23II CGTACG 1 cut(s) 3614
PflMI CCANNNNNTGG 4 cut(s) 3863, 4115, 4464, 5542
PinAI ACCGGT 1 cut(s) 2044
PleI GAGTC 5 cut(s) 2531, 2728, 3465, 4746, 5068
PpsI GAGTC 5 cut(s) 2531, 2728, 3465, 4746, 5068
Ppu21I YACGTR 2 cut(s) 842, 5156
PpuMI RGGWCCY 2 cut(s) 1235, 3829
PscI ACATGT 1 cut(s) 4824
PshBI ATTAAT 1 cut(s) 5616
PsiI TTATAA 4 cut(s) 180, 2088, 5069, 5202
Psp5II RGGWCCY 2 cut(s) 1235, 3829
Psp6I CCWGG 8 cut(s) 793, 862, 915, 1384, 3411, 4336, 4838, 5904
PspEI GGTNACC 1 cut(s) 697
PspFI CCCAGC 2 cut(s) 2408, 5164
PspGI CCWGG 8 cut(s) 793, 862, 915, 1384, 3411, 4336, 4838, 5904
PspLI CGTACG 1 cut(s) 3614
PspN4I GGNNCC 9 cut(s) 1676, 2049, 2361, 3831, 3978, 4126, 4732, 5486, 5526
PspPPI RGGWCCY 2 cut(s) 1235, 3829
PspXI VCTCGAGB 1 cut(s) 719
PstI CTGCAG 3 cut(s) 1324, 4711, 4924
PstNI CAGNNNCTG 5 cut(s) 2003, 4928, 5504, 5915, 6119
PsuI RGATCY 6 cut(s) 1474, 2146, 2359, 4240, 4504, 6421
PvuII CAGCTG 4 cut(s) 1877, 2498, 4928, 6116
RruI TCGCGA 1 cut(s) 2553
RseI CAYNNNNRTG 8 cut(s) 318, 324, 993, 1203, 2796, 4699, 4823, 5754
SapI GCTCTTC 3 cut(s) 618, 647, 4960
ScaI AGTACT 3 cut(s) 1438, 3280, 5320
SchI GAGTC 5 cut(s) 2532, 2728, 3465, 4747, 5069
SduI GDGCHC 8 cut(s) 916, 2415, 2810, 3196, 3595, 3754, 4129, 4706
SexAI ACCWGGT 2 cut(s) 862, 1384
SfcI CTRYAG 8 cut(s) 114, 933, 1320, 1710, 3138, 4101, 4707, 4920
Sfr274I CTCGAG 3 cut(s) 719, 3582, 5622
SfuI TTCGAA 2 cut(s) 50, 1145
SinI GGWCC 4 cut(s) 1235, 1267, 3409, 3829
SlaI CTCGAG 3 cut(s) 719, 3582, 5622
SmiMI CAYNNNNRTG 8 cut(s) 318, 324, 993, 1203, 2796, 4699, 4823, 5754
SmlI CTYRAG 8 cut(s) 719, 1130, 2264, 3582, 3953, 5186, 5622, 6014
SmoI CTYRAG 8 cut(s) 719, 1130, 2264, 3582, 3953, 5186, 5622, 6014
SpeI ACTAGT 1 cut(s) 3349
SseBI AGGCCT 1 cut(s) 3416
SspI AATATT 4 cut(s) 2208, 4363, 5095, 6148
StuI AGGCCT 1 cut(s) 3416
StyI CCWWGG 7 cut(s) 1252, 1544, 1678, 1948, 2134, 2935, 5749
TaiI ACGT 6 cut(s) 777, 844, 2944, 4615, 5158, 5417
TatI WGTACW 9 cut(s) 106, 1436, 1582, 2182, 2632, 3278, 4482, 5318, 5463
TauI GCSGC 5 cut(s) 221, 606, 5695, 5991, 6231
TseFI GTSAC 5 cut(s) 1324, 2314, 3157, 4913, 5061
Tsp45I GTSAC 5 cut(s) 1324, 2314, 3157, 4913, 5061
TspGWI ACGGA 7 cut(s) 12, 73, 1259, 2188, 2858, 5874, 6145
Van91I CCANNNNNTGG 4 cut(s) 3863, 4115, 4464, 5542
Vha464I CTTAAG 3 cut(s) 2264, 3953, 6014
VneI GTGCAC 3 cut(s) 2806, 3192, 3750
VpaK11BI GGWCC 4 cut(s) 1235, 1267, 3409, 3829
VspI ATTAAT 1 cut(s) 5616
XagI CCTNNNNNAGG 1 cut(s) 2338
XbaI TCTAGA 3 cut(s) 769, 1477, 2893
XceI RCATGY 4 cut(s) 747, 2252, 4828, 6359
XcmI CCANNNNNNNNNTGG 1 cut(s) 1336
XhoI CTCGAG 3 cut(s) 719, 3582, 5622
XmiI GTMKAC 1 cut(s) 4197
XmnI GAANNNNTTC 2 cut(s) 636, 4348
ZrmI AGTACT 3 cut(s) 1438, 3280, 5320
Zsp2I ATGCAT 1 cut(s) 6357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.