Rmu_sc0001962.1_g000032

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001962.1
OG0018639
Physical Location & Seq
Forward (+)
133057 .. 134223
1167 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 666 bp
atggcacgatctgagattgataaccacatagggcagagtaggctaattgaggaatcagaccttgacaagcttccctatcttagatgcatcatagatgaatcatcagagaacaccatcggaggcttccatgttccctgtggaagaatgatagtggtaatgtacagtacagaacgcgcctcacgcgcggagaaaaatgagagagagagagcgtcttcgagtgcgacctctcccggccgaacgccaccggctccctgccgctgcgttccggtccgggagatgaagttttccctggctagcggcatggatctctggcggaggcttggttcacggatggtggaaggtggtttgacgtcggacggggcagatcgggtgagatctcctcctattgggtcatgggtgagatctgacaaggaacagcgaaggggtgattgccagcggctgttcctggcgtcacggatggttcagggtgtacggaggtggctggggcttcgatcccagggcagggacgagctcggagatggttcgcccttttttgggcttcaatctcggacatcgaccaggtttgggggtggccggctttgtggtgcggcgatgcggtggtgtaggaatgcgttgctgcaacgtggtggtgcaaggcggcggtgtggtgacgatggagagtgctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

221

Amino Acids

24.61

Weight (kDa)

9.75

Isoelectric Point (pI)

79.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018639)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0216911
rosa_multiflora Rmu_sc0001962.1_g000032
rosa_roxburghii Rroxscaffold_4G00310480 Rroxscaffold_4G00311460
rosa_rugosa Rorug02G0550200 Rorug03G0263200
rosa_samantha Rh1AG256000 Rh2BG061600 Rh2BG373300 Rh4BG201700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 353
AccII CGCG 3 cut(s) 174, 183, 185
AciI CCGC 9 cut(s) 185, 256, 297, 313, 436, 587, 595, 637, 640
AclWI GGATC 2 cut(s) 312, 486
AcoI YGGCCR 2 cut(s) 232, 571
AcyI GRCGYC 2 cut(s) 350, 449
AfaI GTAC 3 cut(s) 161, 166, 471
AfiI CCNNNNNNNGG 6 cut(s) 386, 501, 502, 533, 534, 564
AgsI TTSAA 1 cut(s) 542
AjnI CCWGG 4 cut(s) 288, 444, 495, 557
AloI GAACNNNNNNTCC 2 cut(s) 307, 339
AluBI AGCT 2 cut(s) 70, 511
AluI AGCT 2 cut(s) 70, 511
Alw21I GWGCWC 1 cut(s) 513
AlwI GGATC 2 cut(s) 312, 486
AlwNI CAGNNNCTG 1 cut(s) 439
AoxI GGCC 2 cut(s) 232, 571
ApeKI GCWGC 2 cut(s) 258, 616
AspLEI GCGC 2 cut(s) 176, 185
AspS9I GGNCC 1 cut(s) 268
AsuC2I CCSGG 2 cut(s) 231, 272
AsuHPI GGTGA 4 cut(s) 382, 409, 437, 659
AsuNHI GCTAGC 1 cut(s) 293
AvaII GGWCC 1 cut(s) 268
BanII GRGCYC 1 cut(s) 513
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 513
BbvI GCAGC 2 cut(s) 245, 603
BccI CCATC 5 cut(s) 122, 325, 451, 512, 647
BciT130I CCWGG 4 cut(s) 290, 446, 497, 559
BcnI CCSGG 2 cut(s) 231, 272
BfaI CTAG 2 cut(s) 294, 664
BglII AGATCT 2 cut(s) 374, 401
BisI GCNGC 7 cut(s) 256, 259, 298, 437, 588, 617, 638
BlsI GCNGC 7 cut(s) 257, 260, 299, 438, 589, 618, 639
Bme1390I CCNGG 6 cut(s) 231, 272, 290, 446, 497, 559
Bme18I GGWCC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 6 cut(s) 231, 272, 290, 446, 497, 559
BmsI GCATC 3 cut(s) 74, 96, 582
BmtI GCTAGC 1 cut(s) 297
BpiI GAAGAC 1 cut(s) 204
BplI GAGNNNNNCTC 2 cut(s) 364, 396
BpuMI CCSGG 2 cut(s) 231, 272
BsaHI GRCGYC 2 cut(s) 350, 449
BsaJI CCNNGG 3 cut(s) 288, 495, 496
BsaWI WCCGGW 1 cut(s) 265
BsaXI ACNNNNNCTCC 4 cut(s) 266, 296, 307, 337
Bsc4I CCNNNNNNNGG 6 cut(s) 386, 501, 502, 533, 534, 564
Bse118I RCCGGY 2 cut(s) 244, 573
BseBI CCWGG 4 cut(s) 290, 446, 497, 559
BseDI CCNNGG 3 cut(s) 288, 495, 496
BseGI GGATG 2 cut(s) 336, 462
BseLI CCNNNNNNNGG 6 cut(s) 386, 501, 502, 533, 534, 564
BseRI GAGGAG 1 cut(s) 369
BseX3I CGGCCG 1 cut(s) 232
BseXI GCAGC 2 cut(s) 245, 603
BseYI CCCAGC 1 cut(s) 481
Bsh1236I CGCG 3 cut(s) 174, 183, 185
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 2 cut(s) 234, 573
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 1 cut(s) 513
BsiSI CCGG 5 cut(s) 231, 245, 266, 271, 574
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 6 cut(s) 386, 501, 502, 533, 534, 564
BsmFI GGGAC 1 cut(s) 518
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 2 cut(s) 234, 573
Bsp1286I GDGCHC 1 cut(s) 513
Bsp1407I TGTACA 1 cut(s) 159
Bsp143I GATC 6 cut(s) 8, 304, 364, 374, 401, 491
BspACI CCGC 9 cut(s) 185, 256, 297, 313, 436, 587, 595, 637, 640
BspANI GGCC 2 cut(s) 234, 573
BspCNI CTCAG 1 cut(s) 4
BspFNI CGCG 3 cut(s) 174, 183, 185
BspLI GGNNCC 1 cut(s) 249
BspOI GCTAGC 1 cut(s) 297
BspPI GGATC 2 cut(s) 312, 486
BsrFI RCCGGY 2 cut(s) 244, 573
BsrGI TGTACA 1 cut(s) 159
BssAI RCCGGY 2 cut(s) 244, 573
BssECI CCNNGG 3 cut(s) 288, 495, 496
BssMI GATC 6 cut(s) 8, 304, 364, 374, 401, 491
BssNI GRCGYC 2 cut(s) 350, 449
Bst2UI CCWGG 4 cut(s) 290, 446, 497, 559
Bst4CI ACNGT 1 cut(s) 164
BstACI GRCGYC 2 cut(s) 350, 449
BstAUI TGTACA 1 cut(s) 159
BstC8I GCNNGC 3 cut(s) 295, 434, 575
BstDEI CTNAG 2 cut(s) 12, 80
BstF5I GGATG 2 cut(s) 336, 462
BstFNI CGCG 3 cut(s) 174, 183, 185
BstHHI GCGC 2 cut(s) 176, 185
BstKTI GATC 6 cut(s) 11, 307, 367, 377, 404, 494
BstMBI GATC 6 cut(s) 8, 304, 364, 374, 401, 491
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 3 cut(s) 40, 180, 182
BstNI CCWGG 4 cut(s) 290, 446, 497, 559
BstSCI CCNGG 6 cut(s) 229, 270, 288, 444, 495, 557
BstUI CGCG 3 cut(s) 174, 183, 185
BstV1I GCAGC 2 cut(s) 245, 603
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 3 cut(s) 304, 374, 401
BstYI RGATCY 3 cut(s) 304, 374, 401
BstZI CGGCCG 1 cut(s) 232
BsuRI GGCC 2 cut(s) 234, 573
BtgZI GCGATG 1 cut(s) 605
BtsCI GGATG 2 cut(s) 336, 462
Cac8I GCNNGC 3 cut(s) 295, 434, 575
CaiI CAGNNNCTG 1 cut(s) 439
CfoI GCGC 2 cut(s) 176, 185
Cfr10I RCCGGY 2 cut(s) 244, 573
Cfr13I GGNCC 1 cut(s) 268
CpoI CGGWCCG 1 cut(s) 268
CseI GACGC 2 cut(s) 198, 438
CsiI ACCWGGT 1 cut(s) 557
Csp6I GTAC 3 cut(s) 160, 165, 470
CspI CGGWCCG 1 cut(s) 268
CviAII CATG 3 cut(s) 128, 301, 393
CviQI GTAC 3 cut(s) 160, 165, 470
DdeI CTNAG 2 cut(s) 12, 80
DpnI GATC 6 cut(s) 10, 306, 366, 376, 403, 493
DpnII GATC 6 cut(s) 8, 304, 364, 374, 401, 491
EaeI YGGCCR 2 cut(s) 232, 571
EagI CGGCCG 1 cut(s) 232
EciI GGCGGA 1 cut(s) 328
Ecl136II GAGCTC 1 cut(s) 511
EclXI CGGCCG 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 513
Eco47I GGWCC 1 cut(s) 268
Eco52I CGGCCG 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 511
EcoICRI GAGCTC 1 cut(s) 511
EcoRII CCWGG 4 cut(s) 288, 444, 495, 557
EcoT22I ATGCAT 1 cut(s) 89
EcoT38I GRGCYC 1 cut(s) 513
FaeI CATG 3 cut(s) 131, 304, 396
FaiI YATR 5 cut(s) 29, 92, 129, 302, 394
FaqI GGGAC 1 cut(s) 518
FatI CATG 3 cut(s) 127, 300, 392
Fnu4HI GCNGC 7 cut(s) 256, 259, 298, 437, 588, 617, 638
FokI GGATG 2 cut(s) 343, 469
FriOI GRGCYC 1 cut(s) 513
Fsp4HI GCNGC 7 cut(s) 256, 259, 298, 437, 588, 617, 638
FspBI CTAG 2 cut(s) 294, 664
GlaI GCGC 2 cut(s) 175, 184
GluI GCNGC 7 cut(s) 256, 259, 298, 437, 588, 617, 638
GsaI CCCAGC 1 cut(s) 485
HaeIII GGCC 2 cut(s) 234, 573
HapII CCGG 5 cut(s) 231, 245, 266, 271, 574
HgaI GACGC 2 cut(s) 198, 438
HhaI GCGC 2 cut(s) 176, 185
Hin1I GRCGYC 2 cut(s) 350, 449
Hin1II CATG 3 cut(s) 131, 304, 396
Hin6I GCGC 2 cut(s) 174, 183
HinP1I GCGC 2 cut(s) 174, 183
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 2 cut(s) 53, 98
HpaII CCGG 5 cut(s) 231, 245, 266, 271, 574
HphI GGTGA 4 cut(s) 382, 409, 437, 659
Hpy166II GTNNAC 2 cut(s) 326, 470
Hpy188I TCNGA 8 cut(s) 13, 58, 106, 119, 355, 406, 515, 549
Hpy8I GTNNAC 2 cut(s) 326, 470
Hpy99I CGWCG 1 cut(s) 355
HpyAV CCTTC 2 cut(s) 332, 414
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4IV ACGT 2 cut(s) 350, 622
HpyCH4V TGCA 3 cut(s) 87, 619, 632
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 180, 182
HpyF3I CTNAG 2 cut(s) 12, 80
HpySE526I ACGT 2 cut(s) 350, 622
Hsp92I GRCGYC 2 cut(s) 350, 449
Hsp92II CATG 3 cut(s) 131, 304, 396
HspAI GCGC 2 cut(s) 174, 183
KroI GCCGGC 1 cut(s) 573
KroNI GCCGGC 1 cut(s) 575
Kzo9I GATC 6 cut(s) 8, 304, 364, 374, 401, 491
LmnI GCTCC 1 cut(s) 253
Lsp1109I GCAGC 2 cut(s) 245, 603
LweI GCATC 3 cut(s) 74, 96, 582
MabI ACCWGGT 1 cut(s) 557
MaeI CTAG 2 cut(s) 294, 664
MaeII ACGT 2 cut(s) 350, 622
MaeIII GTNAC 2 cut(s) 450, 647
MalI GATC 6 cut(s) 10, 306, 366, 376, 403, 493
MboI GATC 6 cut(s) 8, 304, 364, 374, 401, 491
MboII GAAGA 2 cut(s) 153, 204
MflI RGATCY 3 cut(s) 304, 374, 401
MhlI GDGCHC 1 cut(s) 513
MluCI AATT 1 cut(s) 45
MmeI TCCRAC 1 cut(s) 333
MnlI CCTC 7 cut(s) 43, 113, 187, 235, 309, 390, 468
Mph1103I ATGCAT 1 cut(s) 89
MroNI GCCGGC 1 cut(s) 573
MspA1I CMGCKG 2 cut(s) 258, 436
MspI CCGG 5 cut(s) 231, 245, 266, 271, 574
MspR9I CCNGG 6 cut(s) 231, 272, 290, 446, 497, 559
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 4 cut(s) 290, 446, 497, 559
MvnI CGCG 3 cut(s) 174, 183, 185
MwoI GCNNNNNNNGC 3 cut(s) 40, 180, 182
NaeI GCCGGC 1 cut(s) 575
NciI CCSGG 2 cut(s) 231, 272
NdeII GATC 6 cut(s) 8, 304, 364, 374, 401, 491
NgoMIV GCCGGC 1 cut(s) 573
NheI GCTAGC 1 cut(s) 293
NlaIII CATG 3 cut(s) 131, 304, 396
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 2 cut(s) 450, 647
NsiI ATGCAT 1 cut(s) 89
PasI CCCWGGG 1 cut(s) 496
PctI GAATGC 1 cut(s) 613
PdiI GCCGGC 1 cut(s) 575
PfeI GAWTC 2 cut(s) 53, 98
PfoI TCCNGGA 1 cut(s) 270
PkrI GCNGC 7 cut(s) 257, 260, 299, 438, 589, 618, 639
Psp124BI GAGCTC 1 cut(s) 513
Psp6I CCWGG 4 cut(s) 288, 444, 495, 557
PspFI CCCAGC 1 cut(s) 481
PspGI CCWGG 4 cut(s) 288, 444, 495, 557
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 439
PsuI RGATCY 3 cut(s) 304, 374, 401
RsaI GTAC 3 cut(s) 161, 166, 471
RsaNI GTAC 3 cut(s) 160, 165, 470
Rsr2I CGGWCCG 1 cut(s) 268
RsrII CGGWCCG 1 cut(s) 268
SacI GAGCTC 1 cut(s) 513
SatI GCNGC 7 cut(s) 256, 259, 298, 437, 588, 617, 638
Sau3AI GATC 6 cut(s) 8, 304, 364, 374, 401, 491
Sau96I GGNCC 1 cut(s) 268
ScrFI CCNGG 6 cut(s) 231, 272, 290, 446, 497, 559
SduI GDGCHC 1 cut(s) 513
SetI ASST 9 cut(s) 63, 72, 227, 343, 353, 479, 513, 563, 625
SexAI ACCWGGT 1 cut(s) 557
SfaNI GCATC 3 cut(s) 74, 96, 582
SinI GGWCC 1 cut(s) 268
Sse9I AATT 1 cut(s) 45
SsiI CCGC 9 cut(s) 185, 256, 297, 313, 436, 587, 595, 637, 640
SspMI CTAG 2 cut(s) 294, 664
SstI GAGCTC 1 cut(s) 513
StyD4I CCNGG 6 cut(s) 229, 270, 288, 444, 495, 557
TaaI ACNGT 1 cut(s) 164
TaiI ACGT 2 cut(s) 353, 625
TaqI TCGA 3 cut(s) 215, 490, 554
TasI AATT 1 cut(s) 45
TatI WGTACW 2 cut(s) 159, 164
TauI GCSGC 5 cut(s) 258, 300, 439, 590, 640
TfiI GAWTC 2 cut(s) 53, 98
TseFI GTSAC 2 cut(s) 450, 647
TseI GCWGC 2 cut(s) 258, 616
Tsp45I GTSAC 2 cut(s) 450, 647
TspDTI ATGAA 2 cut(s) 111, 293
TspGWI ACGGA 3 cut(s) 343, 469, 487
VpaK11BI GGWCC 1 cut(s) 268
XcmI CCANNNNNNNNNTGG 1 cut(s) 134
XspI CTAG 2 cut(s) 294, 664
ZraI GACGTC 1 cut(s) 351
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.