Rmu_sc0002029.1_g000003

serine-threonine kinase receptor-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002029.1
OG0017828
Physical Location & Seq
Forward (+)
2685 .. 6309
3625 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 810 bp
atggccgccatggctcaggctcgcaccgttgtttgccccggtcatgagcgttcgattacggatctttgttacagcccagatccttgtggtggttctcttctgctcagtgctagcgtggacaggcgggccattctgagagatggagagtgcggagattggattagaacatttgctgaacaaacttcttcagtatggaattgctctttgaatattactgcaacagcttcaacggctgcaactgcctctcactgcaatgctttgctaacaggaggactcggctacactatccgtatatttgatttggatcatccacatggaaaaccagaaatcttacgggagtcaaataaacctcagcgaggatcagttcttactgctgcatggctccacaacgatcaggtcatattgagtacatgtagtcttctacagggtgtcagattatgggatcgaaggagtggtaagatactccgtacactcgagactgagtcaatcgtgagaagttcagaactgagtcaaggttatatcaccactgctgctggctctacggtgacattttgggatgaaaccgactttaggtgcgtgaatcgctacaaaatgccatgcatggtacaatcggcttctttgtatgggaacaagtttattgcagggggagatgatgggcttgttcgtttgtttgatttccatactggtgaagagattgaacaatctaatatgggtgacttcggaaccgttttatgtgtgcgtttctcgcccgatggaaaatcctatgctgcgggatttgatgatggcactgttataatcaagaagttgtag

Protein Analysis

269

Amino Acids

29.36

Weight (kDa)

5.87

Isoelectric Point (pI)

50.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 12 - 197 8.2e-12 WDR5 beta-propeller domain
WD40_Prp19 PF24814 12 - 197 7.5e-07 Prp19 WD40 domain
WD40_Gbeta PF25391 31 - 262 1.9e-08 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 85 - 265 6.5e-17 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 138 - 266 9.9e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 173 - 266 4.5e-06 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 173 - 266 9.5e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017828)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0218071
rosa_laevigata RLG00000002412 RLG00000002416
rosa_multiflora Rmu_sc0000379.1_g000006 Rmu_sc0002029.1_g000003
rosa_roxburghii Rroxscaffold_3G00241420
rosa_rugosa Rorug07G0173500
rosa_samantha Rh7AG316000 Rh7BG306300 Rh7DG314200 Rh7DG314700
rosa_wichuraiana Rw7G026690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 794
AciI CCGC 4 cut(s) 6, 124, 150, 770
AclWI GGATC 5 cut(s) 69, 74, 312, 367, 450
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 171
AfaI GTAC 3 cut(s) 409, 469, 606
AfiI CCNNNNNNNGG 3 cut(s) 89, 356, 570
AflIII ACRYGT 1 cut(s) 410
AgsI TTSAA 3 cut(s) 208, 228, 698
AluBI AGCT 1 cut(s) 224
AluI AGCT 1 cut(s) 224
Alw26I GTCTC 1 cut(s) 470
AlwI GGATC 5 cut(s) 69, 74, 312, 367, 450
Ama87I CYCGRG 1 cut(s) 473
AoxI GGCC 2 cut(s) 3, 126
ApeKI GCWGC 4 cut(s) 233, 374, 530, 767
AspS9I GGNCC 1 cut(s) 126
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 4 cut(s) 514, 556, 698, 725
AsuNHI GCTAGC 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 473
BbsI GAAGAC 1 cut(s) 410
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 4 cut(s) 220, 361, 517, 754
BccI CCATC 4 cut(s) 134, 647, 746, 776
BceAI ACGGC 1 cut(s) 246
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 470
BfaI CTAG 1 cut(s) 111
BfmI CTRYAG 1 cut(s) 422
BglI GCCNNNNNGGC 1 cut(s) 11
BisI GCNGC 5 cut(s) 6, 234, 375, 531, 768
BlsI GCNGC 5 cut(s) 7, 235, 376, 532, 769
Bme1390I CCNGG 1 cut(s) 39
BmeT110I CYCGRG 1 cut(s) 473
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 2 cut(s) 383, 724
BmrFI CCNGG 1 cut(s) 39
BmtI GCTAGC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 410
Bpu10I CCTNAGC 2 cut(s) 15, 351
BpuMI CCSGG 1 cut(s) 39
BsaBI GATNNNNATC 1 cut(s) 303
BsaJI CCNNGG 2 cut(s) 9, 37
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 356, 570
Bse1I ACTGG 1 cut(s) 688
Bse3DI GCAATG 1 cut(s) 259
Bse8I GATNNNNATC 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 9, 37
BseGI GGATG 2 cut(s) 307, 562
BseJI GATNNNNATC 1 cut(s) 303
BseLI CCNNNNNNNGG 3 cut(s) 89, 356, 570
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 6 cut(s) 29, 118, 125, 365, 471, 497
BseNI ACTGG 1 cut(s) 688
BseXI GCAGC 4 cut(s) 220, 361, 517, 754
BshFI GGCC 2 cut(s) 5, 128
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 3 cut(s) 89, 356, 570
BsmAI GTCTC 1 cut(s) 470
BsnI GGCC 2 cut(s) 5, 128
BsoBI CYCGRG 1 cut(s) 473
Bsp143I GATC 6 cut(s) 61, 79, 304, 359, 391, 442
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 4 cut(s) 6, 124, 150, 770
BspANI GGCC 2 cut(s) 5, 128
BspCNI CTCAG 6 cut(s) 28, 117, 126, 364, 472, 498
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 2 cut(s) 383, 724
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 5 cut(s) 69, 74, 312, 367, 450
BsrDI GCAATG 1 cut(s) 259
BsrI ACTGG 1 cut(s) 688
BssECI CCNNGG 2 cut(s) 9, 37
BssMI GATC 6 cut(s) 61, 79, 304, 359, 391, 442
BssT1I CCWWGG 1 cut(s) 9
Bst4CI ACNGT 4 cut(s) 28, 544, 727, 790
Bst6I CTCTTC 2 cut(s) 102, 684
BstC8I GCNNGC 4 cut(s) 22, 112, 126, 535
BstDEI CTNAG 6 cut(s) 15, 104, 134, 351, 480, 506
BstDSI CCRYGG 1 cut(s) 9
BstENI CCTNNNNNAGG 1 cut(s) 354
BstF5I GGATG 2 cut(s) 307, 562
BstKTI GATC 6 cut(s) 64, 82, 307, 362, 394, 445
BstMAI GTCTC 1 cut(s) 470
BstMBI GATC 6 cut(s) 61, 79, 304, 359, 391, 442
BstMWI GCNNNNNNNGC 5 cut(s) 11, 230, 239, 582, 745
BstNSI RCATGY 1 cut(s) 414
BstSCI CCNGG 1 cut(s) 37
BstSFI CTRYAG 1 cut(s) 422
BstV1I GCAGC 4 cut(s) 220, 361, 517, 754
BstV2I GAAGAC 1 cut(s) 410
BstX2I RGATCY 2 cut(s) 61, 79
BstYI RGATCY 2 cut(s) 61, 79
BsuRI GGCC 2 cut(s) 5, 128
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 2 cut(s) 307, 562
BtsI GCAGTG 2 cut(s) 247, 525
BtsIMutI CAGTG 4 cut(s) 112, 247, 525, 786
Cac8I GCNNGC 4 cut(s) 22, 112, 126, 535
CciI TCATGA 1 cut(s) 43
Cfr13I GGNCC 1 cut(s) 126
Csp6I GTAC 3 cut(s) 408, 468, 605
CviAII CATG 7 cut(s) 10, 44, 314, 378, 411, 597, 601
CviQI GTAC 3 cut(s) 408, 468, 605
DdeI CTNAG 6 cut(s) 15, 104, 134, 351, 480, 506
DpnI GATC 6 cut(s) 63, 81, 306, 361, 393, 444
DpnII GATC 6 cut(s) 61, 79, 304, 359, 391, 442
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 102, 684
EarI CTCTTC 2 cut(s) 102, 684
Eco130I CCWWGG 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 171
Eco88I CYCGRG 1 cut(s) 473
EcoNI CCTNNNNNAGG 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 602
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 7 cut(s) 13, 47, 317, 381, 414, 600, 604
FatI CATG 7 cut(s) 9, 43, 313, 377, 410, 596, 600
FauI CCCGC 2 cut(s) 117, 763
Fnu4HI GCNGC 5 cut(s) 6, 234, 375, 531, 768
FokI GGATG 2 cut(s) 294, 569
Fsp4HI GCNGC 5 cut(s) 6, 234, 375, 531, 768
FspBI CTAG 1 cut(s) 111
GluI GCNGC 5 cut(s) 6, 234, 375, 531, 768
HaeIII GGCC 2 cut(s) 5, 128
HapII CCGG 1 cut(s) 39
Hin1II CATG 7 cut(s) 13, 47, 317, 381, 414, 600, 604
HinfI GANTC 5 cut(s) 273, 338, 482, 508, 580
HpaII CCGG 1 cut(s) 39
HphI GGTGA 4 cut(s) 514, 556, 698, 725
Hpy166II GTNNAC 2 cut(s) 118, 470
Hpy188I TCNGA 4 cut(s) 135, 434, 502, 722
Hpy188III TCNNGA 4 cut(s) 44, 475, 490, 799
Hpy8I GTNNAC 2 cut(s) 118, 470
HpyAV CCTTC 1 cut(s) 441
HpyCH4III ACNGT 4 cut(s) 28, 544, 727, 790
HpyCH4V TGCA 6 cut(s) 218, 236, 252, 377, 600, 641
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 230, 239, 582, 745
HpyF3I CTNAG 6 cut(s) 15, 104, 134, 351, 480, 506
Hsp92II CATG 7 cut(s) 13, 47, 317, 381, 414, 600, 604
Kzo9I GATC 6 cut(s) 61, 79, 304, 359, 391, 442
LmnI GCTCC 1 cut(s) 387
Lsp1109I GCAGC 4 cut(s) 220, 361, 517, 754
MaeI CTAG 1 cut(s) 111
MaeIII GTNAC 3 cut(s) 68, 544, 713
MalI GATC 6 cut(s) 63, 81, 306, 361, 393, 444
MboI GATC 6 cut(s) 61, 79, 304, 359, 391, 442
MboII GAAGA 4 cut(s) 89, 177, 410, 701
MflI RGATCY 2 cut(s) 61, 79
MluCI AATT 1 cut(s) 196
MlyI GAGTC 4 cut(s) 267, 347, 491, 517
MnlI CCTC 4 cut(s) 253, 263, 350, 360
Mph1103I ATGCAT 1 cut(s) 602
MslI CAYNNNNRTG 3 cut(s) 252, 312, 684
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 1 cut(s) 39
MwoI GCNNNNNNNGC 5 cut(s) 11, 230, 239, 582, 745
NciI CCSGG 1 cut(s) 39
NcoI CCATGG 1 cut(s) 9
NdeII GATC 6 cut(s) 61, 79, 304, 359, 391, 442
NheI GCTAGC 1 cut(s) 110
NlaIII CATG 7 cut(s) 13, 47, 317, 381, 414, 600, 604
NlaIV GGNNCC 2 cut(s) 383, 724
NmeAIII GCCGAG 1 cut(s) 255
NmuCI GTSAC 2 cut(s) 544, 713
NsiI ATGCAT 1 cut(s) 602
NspI RCATGY 1 cut(s) 414
PaeR7I CTCGAG 1 cut(s) 473
PagI TCATGA 1 cut(s) 43
PciI ACATGT 1 cut(s) 410
PfeI GAWTC 1 cut(s) 580
PflFI GACNNNGTC 1 cut(s) 481
PkrI GCNGC 5 cut(s) 7, 235, 376, 532, 769
PleI GAGTC 4 cut(s) 267, 346, 490, 516
PpsI GAGTC 4 cut(s) 267, 346, 490, 516
PscI ACATGT 1 cut(s) 410
PsiI TTATAA 1 cut(s) 794
PspN4I GGNNCC 2 cut(s) 383, 724
PspPI GGNCC 1 cut(s) 126
PsuI RGATCY 2 cut(s) 61, 79
PsyI GACNNNGTC 1 cut(s) 481
RsaI GTAC 3 cut(s) 409, 469, 606
RsaNI GTAC 3 cut(s) 408, 468, 605
RseI CAYNNNNRTG 3 cut(s) 252, 312, 684
SatI GCNGC 5 cut(s) 6, 234, 375, 531, 768
Sau3AI GATC 6 cut(s) 61, 79, 304, 359, 391, 442
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 4 cut(s) 267, 347, 491, 517
ScrFI CCNGG 1 cut(s) 39
SetI ASST 5 cut(s) 226, 352, 399, 517, 575
SfcI CTRYAG 1 cut(s) 422
Sfr274I CTCGAG 1 cut(s) 473
SlaI CTCGAG 1 cut(s) 473
SmiMI CAYNNNNRTG 3 cut(s) 252, 312, 684
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 1 cut(s) 196
SsiI CCGC 4 cut(s) 6, 124, 150, 770
SspI AATATT 1 cut(s) 211
SspMI CTAG 1 cut(s) 111
StyD4I CCNGG 1 cut(s) 37
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 4 cut(s) 28, 544, 727, 790
TaqI TCGA 3 cut(s) 53, 445, 474
TasI AATT 1 cut(s) 196
TatI WGTACW 1 cut(s) 407
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 580
TscAI CASTG 4 cut(s) 112, 254, 532, 793
TseFI GTSAC 2 cut(s) 544, 713
TseI GCWGC 4 cut(s) 233, 374, 530, 767
Tsp45I GTSAC 2 cut(s) 544, 713
TspDTI ATGAA 1 cut(s) 573
TspGWI ACGGA 3 cut(s) 74, 278, 455
TspRI CASTG 4 cut(s) 112, 254, 532, 793
Tth111I GACNNNGTC 1 cut(s) 481
XagI CCTNNNNNAGG 1 cut(s) 354
XceI RCATGY 1 cut(s) 414
XhoI CTCGAG 1 cut(s) 473
XspI CTAG 1 cut(s) 111
Zsp2I ATGCAT 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.