Rmu_sc0002034.1_g000010

DEHYDRATION-INDUCED 19-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002034.1
OG0012591
Physical Location & Seq
Forward (+)
28932 .. 31708
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002034.1_g000010.1.cds

Sequence Viewer

Length: 645 bp
atggattccgagttctggacctcccgccttgccgccgcgaaacgtcagtacacgctgcagcagcatcaccagagctcccacttggatcggctgagcatggatgatttcgaggtggaagacgaggtccgaccggaattcccgtgtccgtattgctacgaggacttcgacatcgcgtcgttgtgttcgcacctggaggacgagcacccgtgcgaatctagagtcaccgtttgtccgatttgctcaattaaagttacacgggacatgttaagtcatattacactgcaacatgggcactttcaagcactgtctctccttggtcgggatcttcgtgaggctcatctccaggtgcttttagggagtgggggatatcgatcagagaatgctaatgtcactaatgcagctactgatccattcctgtcatcacttattttgaatttccctgcatcaggagcagatgaaatttcaaaatctgtggtaacaactgctgaggacactcctgcaaagaaggtggcgccatcacatatttggaaatcaagttttgatccttccttgagtcatgaagagcgggagaaaaggatacaacaagctacggggagagctggttttgtgcaagatctgtttctctctacaatgttaagtgactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Di19_C zf-Di19

Protein Analysis

214

Amino Acids

24.02

Weight (kDa)

5.21

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 511
AccBSI CCGCTC 1 cut(s) 565
AccII CGCG 2 cut(s) 38, 173
AciI CCGC 4 cut(s) 25, 33, 36, 565
AclWI GGATC 4 cut(s) 93, 330, 401, 536
AcsI RAATTY 3 cut(s) 134, 433, 459
AcyI GRCGYC 1 cut(s) 512
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 3 cut(s) 15, 319, 446
AflIII ACRYGT 1 cut(s) 261
AgsI TTSAA 3 cut(s) 299, 433, 465
AhdI GACNNNNNGTC 1 cut(s) 172
AjnI CCWGG 2 cut(s) 189, 342
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 4 cut(s) 75, 401, 587, 599
AluI AGCT 4 cut(s) 75, 401, 587, 599
Alw21I GWGCWC 2 cut(s) 77, 204
Alw26I GTCTC 1 cut(s) 312
AlwI GGATC 4 cut(s) 93, 330, 401, 536
AlwNI CAGNNNCTG 1 cut(s) 404
ApeKI GCWGC 4 cut(s) 55, 58, 61, 398
ApoI RAATTY 3 cut(s) 134, 433, 459
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
AspLEI GCGC 1 cut(s) 514
AspS9I GGNCC 2 cut(s) 18, 124
AsuHPI GGTGA 2 cut(s) 59, 214
AvaII GGWCC 2 cut(s) 18, 124
BaeGI GKGCMC 1 cut(s) 294
BanI GGYRCC 1 cut(s) 511
BanII GRGCYC 1 cut(s) 77
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 2 cut(s) 77, 204
BbvCI CCTCAGC 1 cut(s) 486
BbvI GCAGC 4 cut(s) 42, 70, 73, 410
BccI CCATC 1 cut(s) 523
BciT130I CCWGG 2 cut(s) 191, 344
BciVI GTATCC 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 312
BfaI CTAG 1 cut(s) 216
BfmI CTRYAG 1 cut(s) 56
BfoI RGCGCY 1 cut(s) 515
BfuI GTATCC 1 cut(s) 570
BglII AGATCT 1 cut(s) 613
BisI GCNGC 6 cut(s) 33, 36, 56, 59, 62, 399
BlpI GCTNAGC 1 cut(s) 92
BlsI GCNGC 6 cut(s) 34, 37, 57, 60, 63, 400
Bme1390I CCNGG 2 cut(s) 191, 344
Bme18I GGWCC 2 cut(s) 18, 124
BmeRI GACNNNNNGTC 1 cut(s) 172
BmgT120I GGNCC 2 cut(s) 18, 124
BmiI GGNNCC 1 cut(s) 513
BmrFI CCNGG 2 cut(s) 191, 344
BmsI GCATC 2 cut(s) 73, 452
BpiI GAAGAC 1 cut(s) 123
BpmI CTGGAG 2 cut(s) 212, 326
Bpu10I CCTNAGC 1 cut(s) 486
Bpu1102I GCTNAGC 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 571
Bsa29I ATCGAT 1 cut(s) 370
BsaBI GATNNNNATC 1 cut(s) 370
BsaHI GRCGYC 1 cut(s) 512
BsaJI CCNNGG 1 cut(s) 313
BsaWI WCCGGW 1 cut(s) 130
BsaXI ACNNNNNCTCC 6 cut(s) 59, 89, 294, 324, 586, 616
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 319, 446
Bse8I GATNNNNATC 1 cut(s) 370
BseBI CCWGG 2 cut(s) 191, 344
BseCI ATCGAT 1 cut(s) 370
BseDI CCNNGG 1 cut(s) 313
BseGI GGATG 1 cut(s) 106
BseJI GATNNNNATC 1 cut(s) 370
BseLI CCNNNNNNNGG 3 cut(s) 15, 319, 446
BseMII CTCAG 2 cut(s) 83, 477
BseSI GKGCMC 1 cut(s) 294
BseXI GCAGC 4 cut(s) 42, 70, 73, 410
Bsh1236I CGCG 2 cut(s) 38, 173
Bsh1285I CGRYCG 1 cut(s) 131
BshNI GGYRCC 1 cut(s) 511
BshVI ATCGAT 1 cut(s) 370
BsiEI CGRYCG 1 cut(s) 131
BsiHKAI GWGCWC 2 cut(s) 77, 204
BsiSI CCGG 1 cut(s) 131
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 3 cut(s) 15, 319, 446
BsmAI GTCTC 1 cut(s) 312
BsmFI GGGAC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 385
Bsp1286I GDGCHC 3 cut(s) 77, 204, 294
Bsp143I GATC 6 cut(s) 85, 322, 371, 406, 541, 613
Bsp1720I GCTNAGC 1 cut(s) 92
BspACI CCGC 4 cut(s) 25, 33, 36, 565
BspCNI CTCAG 2 cut(s) 84, 478
BspDI ATCGAT 1 cut(s) 370
BspFNI CGCG 2 cut(s) 38, 173
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 1 cut(s) 513
BspMAI CTGCAG 1 cut(s) 60
BspPI GGATC 4 cut(s) 93, 330, 401, 536
BspQI GCTCTTC 1 cut(s) 555
BspT107I GGYRCC 1 cut(s) 511
BsrBI CCGCTC 1 cut(s) 565
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 6 cut(s) 85, 322, 371, 406, 541, 613
BssNI GRCGYC 1 cut(s) 512
BssT1I CCWWGG 1 cut(s) 313
Bst2UI CCWGG 2 cut(s) 191, 344
Bst4CI ACNGT 2 cut(s) 226, 306
Bst6I CTCTTC 1 cut(s) 555
BstACI GRCGYC 1 cut(s) 512
BstDEI CTNAG 2 cut(s) 92, 486
BstENI CCTNNNNNAGG 1 cut(s) 444
BstF5I GGATG 1 cut(s) 106
BstFNI CGCG 2 cut(s) 38, 173
BstH2I RGCGCY 1 cut(s) 515
BstHHI GCGC 1 cut(s) 514
BstKTI GATC 6 cut(s) 88, 325, 374, 409, 544, 616
BstMAI GTCTC 1 cut(s) 312
BstMBI GATC 6 cut(s) 85, 322, 371, 406, 541, 613
BstMCI CGRYCG 1 cut(s) 131
BstMWI GCNNNNNNNGC 3 cut(s) 61, 289, 449
BstNI CCWGG 2 cut(s) 191, 344
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 2 cut(s) 189, 342
BstSFI CTRYAG 1 cut(s) 56
BstSLI GKGCMC 1 cut(s) 294
BstUI CGCG 2 cut(s) 38, 173
BstV1I GCAGC 4 cut(s) 42, 70, 73, 410
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 2 cut(s) 322, 613
BstYI RGATCY 2 cut(s) 322, 613
Bsu15I ATCGAT 1 cut(s) 370
BsuI GTATCC 1 cut(s) 570
BsuTUI ATCGAT 1 cut(s) 370
BtgZI GCGATG 1 cut(s) 154
BtsCI GGATG 1 cut(s) 106
BtsI GCAGTG 1 cut(s) 278
BtsIMutI CAGTG 2 cut(s) 278, 302
CaiI CAGNNNCTG 1 cut(s) 404
CciI TCATGA 1 cut(s) 556
CfoI GCGC 1 cut(s) 514
Cfr13I GGNCC 2 cut(s) 18, 124
ClaI ATCGAT 1 cut(s) 370
CseI GACGC 1 cut(s) 162
Csp6I GTAC 1 cut(s) 49
CspCI CAANNNNNGTGG 4 cut(s) 453, 488, 489, 524
CviAII CATG 4 cut(s) 97, 262, 287, 557
CviJI RGCY 6 cut(s) 75, 91, 335, 401, 587, 599
CviKI_1 RGCY 6 cut(s) 75, 91, 335, 401, 587, 599
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 2 cut(s) 92, 486
DinI GGCGCC 1 cut(s) 513
DpnI GATC 6 cut(s) 87, 324, 373, 408, 543, 615
DpnII GATC 6 cut(s) 85, 322, 371, 406, 541, 613
DriI GACNNNNNGTC 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 555
Eam1105I GACNNNNNGTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 555
Ecl136II GAGCTC 1 cut(s) 75
Eco130I CCWWGG 1 cut(s) 313
Eco24I GRGCYC 1 cut(s) 77
Eco32I GATATC 1 cut(s) 368
Eco47I GGWCC 2 cut(s) 18, 124
Eco53kI GAGCTC 1 cut(s) 75
EcoICRI GAGCTC 1 cut(s) 75
EcoNI CCTNNNNNAGG 1 cut(s) 444
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 2 cut(s) 189, 342
EcoRV GATATC 1 cut(s) 368
EcoT14I CCWWGG 1 cut(s) 313
EcoT38I GRGCYC 1 cut(s) 77
EgeI GGCGCC 1 cut(s) 513
EheI GGCGCC 1 cut(s) 513
ErhI CCWWGG 1 cut(s) 313
FaeI CATG 4 cut(s) 100, 265, 290, 560
FaiI YATR 6 cut(s) 98, 263, 273, 288, 522, 558
FaqI GGGAC 1 cut(s) 272
FatI CATG 4 cut(s) 96, 261, 286, 556
FauI CCCGC 2 cut(s) 32, 558
Fnu4HI GCNGC 6 cut(s) 33, 36, 56, 59, 62, 399
FokI GGATG 1 cut(s) 113
FriOI GRGCYC 1 cut(s) 77
Fsp4HI GCNGC 6 cut(s) 33, 36, 56, 59, 62, 399
FspBI CTAG 1 cut(s) 216
GlaI GCGC 1 cut(s) 513
GluI GCNGC 6 cut(s) 33, 36, 56, 59, 62, 399
GsuI CTGGAG 2 cut(s) 212, 326
HaeII RGCGCY 1 cut(s) 515
HapII CCGG 1 cut(s) 131
HgaI GACGC 1 cut(s) 162
HhaI GCGC 1 cut(s) 514
Hin1I GRCGYC 1 cut(s) 512
Hin1II CATG 4 cut(s) 100, 265, 290, 560
Hin6I GCGC 1 cut(s) 512
HinP1I GCGC 1 cut(s) 512
HinfI GANTC 4 cut(s) 5, 212, 219, 553
HpaII CCGG 1 cut(s) 131
HphI GGTGA 2 cut(s) 59, 214
Hpy166II GTNNAC 1 cut(s) 51
Hpy188I TCNGA 4 cut(s) 10, 128, 234, 376
Hpy188III TCNNGA 6 cut(s) 16, 216, 320, 329, 447, 557
Hpy8I GTNNAC 1 cut(s) 51
Hpy99I CGWCG 1 cut(s) 178
HpyAV CCTTC 2 cut(s) 499, 555
HpyCH4III ACNGT 2 cut(s) 226, 306
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 6 cut(s) 58, 283, 398, 443, 500, 610
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 289, 449
HpyF3I CTNAG 2 cut(s) 92, 486
HpySE526I ACGT 1 cut(s) 43
Hsp92I GRCGYC 1 cut(s) 512
Hsp92II CATG 4 cut(s) 100, 265, 290, 560
HspAI GCGC 1 cut(s) 512
KasI GGCGCC 1 cut(s) 511
Kzo9I GATC 6 cut(s) 85, 322, 371, 406, 541, 613
LguI GCTCTTC 1 cut(s) 555
LmnI GCTCC 2 cut(s) 80, 449
Lsp1109I GCAGC 4 cut(s) 42, 70, 73, 410
LweI GCATC 2 cut(s) 73, 452
MaeI CTAG 1 cut(s) 216
MaeII ACGT 1 cut(s) 43
MaeIII GTNAC 5 cut(s) 220, 250, 388, 475, 638
MalI GATC 6 cut(s) 87, 324, 373, 408, 543, 615
MbiI CCGCTC 1 cut(s) 565
MboI GATC 6 cut(s) 85, 322, 371, 406, 541, 613
MboII GAAGA 3 cut(s) 128, 317, 572
MflI RGATCY 2 cut(s) 322, 613
MhlI GDGCHC 3 cut(s) 77, 204, 294
MluCI AATT 4 cut(s) 134, 243, 433, 459
Mly113I GGCGCC 1 cut(s) 512
MlyI GAGTC 2 cut(s) 228, 562
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 7 cut(s) 31, 103, 115, 151, 187, 325, 481
MseI TTAA 3 cut(s) 246, 266, 635
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 2 cut(s) 191, 344
Mva1269I GAATGC 1 cut(s) 385
MvaI CCWGG 2 cut(s) 191, 344
MvnI CGCG 2 cut(s) 38, 173
MwoI GCNNNNNNNGC 3 cut(s) 61, 289, 449
NarI GGCGCC 1 cut(s) 512
NdeII GATC 6 cut(s) 85, 322, 371, 406, 541, 613
NlaIII CATG 4 cut(s) 100, 265, 290, 560
NlaIV GGNNCC 1 cut(s) 513
NmuCI GTSAC 3 cut(s) 220, 388, 638
NspI RCATGY 1 cut(s) 265
PagI TCATGA 1 cut(s) 556
PciI ACATGT 1 cut(s) 261
PciSI GCTCTTC 1 cut(s) 555
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 325
PctI GAATGC 1 cut(s) 385
PfeI GAWTC 2 cut(s) 5, 212
PflFI GACNNNGTC 1 cut(s) 122
PkrI GCNGC 6 cut(s) 34, 37, 57, 60, 63, 400
PleI GAGTC 2 cut(s) 227, 561
PluTI GGCGCC 1 cut(s) 515
PpsI GAGTC 2 cut(s) 227, 561
PscI ACATGT 1 cut(s) 261
Psp124BI GAGCTC 1 cut(s) 77
Psp6I CCWGG 2 cut(s) 189, 342
PspGI CCWGG 2 cut(s) 189, 342
PspN4I GGNNCC 1 cut(s) 513
PspPI GGNCC 2 cut(s) 18, 124
PstI CTGCAG 1 cut(s) 60
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 2 cut(s) 322, 613
PsyI GACNNNGTC 1 cut(s) 122
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SacI GAGCTC 1 cut(s) 77
SapI GCTCTTC 1 cut(s) 555
SaqAI TTAA 3 cut(s) 246, 266, 635
SatI GCNGC 6 cut(s) 33, 36, 56, 59, 62, 399
Sau3AI GATC 6 cut(s) 85, 322, 371, 406, 541, 613
Sau96I GGNCC 2 cut(s) 18, 124
SchI GAGTC 2 cut(s) 228, 562
ScrFI CCNGG 2 cut(s) 191, 344
SduI GDGCHC 3 cut(s) 77, 204, 294
SfaNI GCATC 2 cut(s) 73, 452
SfcI CTRYAG 1 cut(s) 56
SfoI GGCGCC 1 cut(s) 513
SinI GGWCC 2 cut(s) 18, 124
SmlI CTYRAG 1 cut(s) 550
SmoI CTYRAG 1 cut(s) 550
Sse9I AATT 4 cut(s) 134, 243, 433, 459
SsiI CCGC 4 cut(s) 25, 33, 36, 565
SspDI GGCGCC 1 cut(s) 511
SspMI CTAG 1 cut(s) 216
SstI GAGCTC 1 cut(s) 77
StyD4I CCNGG 2 cut(s) 189, 342
StyI CCWWGG 1 cut(s) 313
TaaI ACNGT 2 cut(s) 226, 306
TaiI ACGT 1 cut(s) 46
TaqI TCGA 3 cut(s) 108, 165, 370
TasI AATT 4 cut(s) 134, 243, 433, 459
TatI WGTACW 1 cut(s) 48
TauI GCSGC 2 cut(s) 35, 38
TfiI GAWTC 2 cut(s) 5, 212
Tru1I TTAA 3 cut(s) 246, 266, 635
Tru9I TTAA 3 cut(s) 246, 266, 635
TscAI CASTG 2 cut(s) 285, 309
TseFI GTSAC 3 cut(s) 220, 388, 638
TseI GCWGC 4 cut(s) 55, 58, 61, 398
Tsp45I GTSAC 3 cut(s) 220, 388, 638
TspDTI ATGAA 2 cut(s) 471, 573
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 2 cut(s) 285, 309
Tth111I GACNNNGTC 1 cut(s) 122
VpaK11BI GGWCC 2 cut(s) 18, 124
XagI CCTNNNNNAGG 1 cut(s) 444
XapI RAATTY 3 cut(s) 134, 433, 459
XbaI TCTAGA 1 cut(s) 215
XceI RCATGY 1 cut(s) 265
XcmI CCANNNNNNNNNTGG 1 cut(s) 522
XspI CTAG 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.