Rroxscaffold_1G00066960

DEHYDRATION-INDUCED 19-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012591
Physical Location & Seq
Forward (+)
88383812 .. 88387427
3616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00066960.1

Sequence Viewer

Length: 696 bp
ATGGATTCCGAGTTCTGGACCTCCCGCCTCGCCGCAGCGAAACGTCAGTATACGTTGCAGCACCATCACCAGAGCTCCCACTTGGATCGGCTGAGCATGGATGATTTCGAGGTGGAAGACGAGGTCCGACCGGACTTCCCGTGCCCGTATTGCTACGAGGACTTCGACATCGCGTCGTTGTGTTCGCACCTGGAGGACGAGCACCCGTGCGAATCTAGAGTCACCGTTTGTCCGATTTGCTCAATTAAAGTTACACGGGACATGTTAAGTCATATTACACTGCAACATGGGCACTTGTTCAAGTTGCAGAGACGTCGCAGGTTACGTAGGGTTGCTATTCCTAATAGTCAAGCACTGTCTCTCCTTGGCCGGGATCTTCGTGAGGCTCATCTCCAGGTGCTTTTAGGGAGTGGGGGATATCGATCAGAGAATGCTAATGTGACTAATGCAGCTACTGATCCATTCCTGTCATCACTTATTTTGAATTTCCCTGCATCAGGAGCAGATGAAATTTCAAAATCTGTGGTAACAACTGCTGAGGACACTCCTGCAAAGAAGGTGGCACCATCACATATTTGGAAATCAAGTTTTGATCCTTCCTTGAGTCATGAAGAGCGGGAGAAAAGGATACAACAAGCTATGGGGAGAGCTGGTTTTGTGCAAGATCTGTTTCTCTCTACAATGTTAAGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Di19_C zf-Di19

Protein Analysis

231

Amino Acids

26.16

Weight (kDa)

6.05

Isoelectric Point (pI)

52.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Di19 PF05605 45 - 97 4.7e-23 Drought induced 19 protein (Di19), zinc-binding
Di19_C PF14571 119 - 228 2.1e-30 Stress-induced protein Di19, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 316
Acc36I ACCTGC 1 cut(s) 309
AccB1I GGYRCC 1 cut(s) 562
AccBSI CCGCTC 1 cut(s) 616
AccI GTMKAC 1 cut(s) 50
AccII CGCG 1 cut(s) 173
AciI CCGC 3 cut(s) 25, 33, 616
AclWI GGATC 4 cut(s) 93, 381, 452, 587
AcoI YGGCCR 1 cut(s) 367
AcsI RAATTY 2 cut(s) 484, 510
AcyI GRCGYC 1 cut(s) 313
AfiI CCNNNNNNNGG 3 cut(s) 15, 370, 497
AflIII ACRYGT 1 cut(s) 261
AgsI TTSAA 3 cut(s) 301, 484, 516
AhdI GACNNNNNGTC 1 cut(s) 172
AjnI CCWGG 2 cut(s) 189, 393
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 4 cut(s) 75, 452, 638, 650
AluI AGCT 4 cut(s) 75, 452, 638, 650
Alw21I GWGCWC 2 cut(s) 77, 204
Alw26I GTCTC 2 cut(s) 304, 363
AlwI GGATC 4 cut(s) 93, 381, 452, 587
AlwNI CAGNNNCTG 1 cut(s) 455
AoxI GGCC 1 cut(s) 367
ApeKI GCWGC 3 cut(s) 35, 58, 449
ApoI RAATTY 2 cut(s) 484, 510
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
AspS9I GGNCC 2 cut(s) 18, 124
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 2 cut(s) 59, 214
AvaII GGWCC 2 cut(s) 18, 124
BaeGI GKGCMC 2 cut(s) 146, 294
BanI GGYRCC 1 cut(s) 562
BanII GRGCYC 1 cut(s) 77
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 2 cut(s) 77, 204
BbvCI CCTCAGC 1 cut(s) 537
BbvI GCAGC 3 cut(s) 47, 70, 461
BccI CCATC 2 cut(s) 72, 574
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 2 cut(s) 191, 395
BciVI GTATCC 1 cut(s) 621
BcnI CCSGG 1 cut(s) 371
BcoDI GTCTC 2 cut(s) 304, 363
BfaI CTAG 1 cut(s) 216
BfuAI ACCTGC 1 cut(s) 309
BfuI GTATCC 1 cut(s) 621
BglII AGATCT 1 cut(s) 664
BisI GCNGC 4 cut(s) 33, 36, 59, 450
BlpI GCTNAGC 1 cut(s) 92
BlsI GCNGC 4 cut(s) 34, 37, 60, 451
Bme1390I CCNGG 3 cut(s) 191, 371, 395
Bme18I GGWCC 2 cut(s) 18, 124
BmeRI GACNNNNNGTC 1 cut(s) 172
BmgT120I GGNCC 2 cut(s) 18, 124
BmiI GGNNCC 1 cut(s) 564
BmrFI CCNGG 3 cut(s) 191, 371, 395
BmsI GCATC 1 cut(s) 503
BpiI GAAGAC 1 cut(s) 123
BpmI CTGGAG 2 cut(s) 212, 377
Bpu10I CCTNAGC 1 cut(s) 537
Bpu1102I GCTNAGC 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 622
BpuMI CCSGG 1 cut(s) 371
Bsa29I ATCGAT 1 cut(s) 421
BsaAI YACGTR 1 cut(s) 326
BsaBI GATNNNNATC 1 cut(s) 421
BsaHI GRCGYC 1 cut(s) 313
BsaJI CCNNGG 1 cut(s) 364
BsaWI WCCGGW 1 cut(s) 130
BsaXI ACNNNNNCTCC 6 cut(s) 59, 89, 345, 375, 637, 667
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 370, 497
Bse8I GATNNNNATC 1 cut(s) 421
BseBI CCWGG 2 cut(s) 191, 395
BseCI ATCGAT 1 cut(s) 421
BseDI CCNNGG 1 cut(s) 364
BseGI GGATG 1 cut(s) 106
BseJI GATNNNNATC 1 cut(s) 421
BseLI CCNNNNNNNGG 3 cut(s) 15, 370, 497
BseMII CTCAG 2 cut(s) 83, 528
BseSI GKGCMC 2 cut(s) 146, 294
BseXI GCAGC 3 cut(s) 47, 70, 461
Bsh1236I CGCG 1 cut(s) 173
Bsh1285I CGRYCG 1 cut(s) 131
BshFI GGCC 1 cut(s) 369
BshNI GGYRCC 1 cut(s) 562
BshVI ATCGAT 1 cut(s) 421
BsiEI CGRYCG 1 cut(s) 131
BsiHKAI GWGCWC 2 cut(s) 77, 204
BsiSI CCGG 2 cut(s) 131, 370
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 3 cut(s) 15, 370, 497
BsmAI GTCTC 2 cut(s) 304, 363
BsmBI CGTCTC 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 436
BsnI GGCC 1 cut(s) 369
Bsp1286I GDGCHC 4 cut(s) 77, 146, 204, 294
Bsp143I GATC 6 cut(s) 85, 373, 422, 457, 592, 664
Bsp1720I GCTNAGC 1 cut(s) 92
BspACI CCGC 3 cut(s) 25, 33, 616
BspANI GGCC 1 cut(s) 369
BspCNI CTCAG 2 cut(s) 84, 529
BspDI ATCGAT 1 cut(s) 421
BspFNI CGCG 1 cut(s) 173
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 1 cut(s) 564
BspMI ACCTGC 1 cut(s) 309
BspPI GGATC 4 cut(s) 93, 381, 452, 587
BspQI GCTCTTC 1 cut(s) 606
BspT107I GGYRCC 1 cut(s) 562
BsrBI CCGCTC 1 cut(s) 616
BssECI CCNNGG 1 cut(s) 364
BssMI GATC 6 cut(s) 85, 373, 422, 457, 592, 664
BssNAI GTATAC 1 cut(s) 51
BssNI GRCGYC 1 cut(s) 313
BssT1I CCWWGG 1 cut(s) 364
Bst1107I GTATAC 1 cut(s) 51
Bst2UI CCWGG 2 cut(s) 191, 395
Bst4CI ACNGT 2 cut(s) 226, 357
Bst6I CTCTTC 1 cut(s) 606
BstACI GRCGYC 1 cut(s) 313
BstBAI YACGTR 1 cut(s) 326
BstDEI CTNAG 2 cut(s) 92, 537
BstENI CCTNNNNNAGG 1 cut(s) 495
BstF5I GGATG 1 cut(s) 106
BstFNI CGCG 1 cut(s) 173
BstKTI GATC 6 cut(s) 88, 376, 425, 460, 595, 667
BstMAI GTCTC 2 cut(s) 304, 363
BstMBI GATC 6 cut(s) 85, 373, 422, 457, 592, 664
BstMCI CGRYCG 1 cut(s) 131
BstMWI GCNNNNNNNGC 3 cut(s) 150, 289, 500
BstNI CCWGG 2 cut(s) 191, 395
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 3 cut(s) 189, 369, 393
BstSLI GKGCMC 2 cut(s) 146, 294
BstSNI TACGTA 1 cut(s) 326
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 3 cut(s) 47, 70, 461
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 2 cut(s) 373, 664
BstYI RGATCY 2 cut(s) 373, 664
BstZ17I GTATAC 1 cut(s) 51
Bsu15I ATCGAT 1 cut(s) 421
BsuI GTATCC 1 cut(s) 621
BsuRI GGCC 1 cut(s) 369
BsuTUI ATCGAT 1 cut(s) 421
BtgZI GCGATG 1 cut(s) 154
BtsCI GGATG 1 cut(s) 106
BtsI GCAGTG 1 cut(s) 278
BtsIMutI CAGTG 2 cut(s) 278, 353
BveI ACCTGC 1 cut(s) 309
CaiI CAGNNNCTG 1 cut(s) 455
CciI TCATGA 1 cut(s) 607
Cfr13I GGNCC 2 cut(s) 18, 124
ClaI ATCGAT 1 cut(s) 421
CseI GACGC 1 cut(s) 162
CspCI CAANNNNNGTGG 4 cut(s) 504, 539, 540, 575
CviAII CATG 4 cut(s) 97, 262, 287, 608
CviJI RGCY 7 cut(s) 75, 91, 369, 386, 452, 638, 650
CviKI_1 RGCY 7 cut(s) 75, 91, 369, 386, 452, 638, 650
DdeI CTNAG 2 cut(s) 92, 537
DpnI GATC 6 cut(s) 87, 375, 424, 459, 594, 666
DpnII GATC 6 cut(s) 85, 373, 422, 457, 592, 664
DriI GACNNNNNGTC 1 cut(s) 172
EaeI YGGCCR 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 606
Eam1105I GACNNNNNGTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 606
Ecl136II GAGCTC 1 cut(s) 75
Eco105I TACGTA 1 cut(s) 326
Eco130I CCWWGG 1 cut(s) 364
Eco24I GRGCYC 1 cut(s) 77
Eco32I GATATC 1 cut(s) 419
Eco47I GGWCC 2 cut(s) 18, 124
Eco53kI GAGCTC 1 cut(s) 75
EcoICRI GAGCTC 1 cut(s) 75
EcoNI CCTNNNNNAGG 1 cut(s) 495
EcoRII CCWGG 2 cut(s) 189, 393
EcoRV GATATC 1 cut(s) 419
EcoT14I CCWWGG 1 cut(s) 364
EcoT38I GRGCYC 1 cut(s) 77
ErhI CCWWGG 1 cut(s) 364
Esp3I CGTCTC 1 cut(s) 304
FaeI CATG 4 cut(s) 100, 265, 290, 611
FaiI YATR 8 cut(s) 51, 98, 263, 273, 288, 573, 609, 641
FaqI GGGAC 1 cut(s) 272
FatI CATG 4 cut(s) 96, 261, 286, 607
FauI CCCGC 2 cut(s) 32, 609
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 4 cut(s) 33, 36, 59, 450
FokI GGATG 1 cut(s) 113
FriOI GRGCYC 1 cut(s) 77
Fsp4HI GCNGC 4 cut(s) 33, 36, 59, 450
FspBI CTAG 1 cut(s) 216
GluI GCNGC 4 cut(s) 33, 36, 59, 450
GsuI CTGGAG 2 cut(s) 212, 377
HaeIII GGCC 1 cut(s) 369
HapII CCGG 2 cut(s) 131, 370
HgaI GACGC 1 cut(s) 162
Hin1I GRCGYC 1 cut(s) 313
Hin1II CATG 4 cut(s) 100, 265, 290, 611
HinfI GANTC 4 cut(s) 5, 212, 219, 604
HpaII CCGG 2 cut(s) 131, 370
HphI GGTGA 2 cut(s) 59, 214
Hpy166II GTNNAC 1 cut(s) 51
Hpy188I TCNGA 4 cut(s) 10, 128, 234, 427
Hpy188III TCNNGA 5 cut(s) 16, 216, 380, 498, 608
Hpy8I GTNNAC 1 cut(s) 51
Hpy99I CGWCG 2 cut(s) 178, 318
HpyAV CCTTC 2 cut(s) 550, 606
HpyCH4III ACNGT 2 cut(s) 226, 357
HpyCH4IV ACGT 4 cut(s) 43, 53, 313, 325
HpyCH4V TGCA 7 cut(s) 58, 283, 307, 449, 494, 551, 661
HpyF10VI GCNNNNNNNGC 3 cut(s) 150, 289, 500
HpyF3I CTNAG 2 cut(s) 92, 537
HpySE526I ACGT 4 cut(s) 43, 53, 313, 325
Hsp92I GRCGYC 1 cut(s) 313
Hsp92II CATG 4 cut(s) 100, 265, 290, 611
Kzo9I GATC 6 cut(s) 85, 373, 422, 457, 592, 664
LguI GCTCTTC 1 cut(s) 606
LmnI GCTCC 2 cut(s) 80, 500
Lsp1109I GCAGC 3 cut(s) 47, 70, 461
LweI GCATC 1 cut(s) 503
MaeI CTAG 1 cut(s) 216
MaeII ACGT 4 cut(s) 43, 53, 313, 325
MaeIII GTNAC 6 cut(s) 220, 250, 321, 439, 526, 689
MalI GATC 6 cut(s) 87, 375, 424, 459, 594, 666
MbiI CCGCTC 1 cut(s) 616
MboI GATC 6 cut(s) 85, 373, 422, 457, 592, 664
MboII GAAGA 3 cut(s) 128, 368, 623
MflI RGATCY 2 cut(s) 373, 664
MhlI GDGCHC 4 cut(s) 77, 146, 204, 294
MluCI AATT 3 cut(s) 243, 484, 510
MlyI GAGTC 2 cut(s) 228, 613
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 8 cut(s) 31, 38, 103, 115, 151, 187, 376, 532
MseI TTAA 3 cut(s) 246, 266, 686
MspI CCGG 2 cut(s) 131, 370
MspR9I CCNGG 3 cut(s) 191, 371, 395
Mva1269I GAATGC 1 cut(s) 436
MvaI CCWGG 2 cut(s) 191, 395
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 3 cut(s) 150, 289, 500
NciI CCSGG 1 cut(s) 371
NdeII GATC 6 cut(s) 85, 373, 422, 457, 592, 664
NlaIII CATG 4 cut(s) 100, 265, 290, 611
NlaIV GGNNCC 1 cut(s) 564
NmuCI GTSAC 3 cut(s) 220, 439, 689
NspI RCATGY 1 cut(s) 265
PagI TCATGA 1 cut(s) 607
PciI ACATGT 1 cut(s) 261
PciSI GCTCTTC 1 cut(s) 606
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 322
PctI GAATGC 1 cut(s) 436
PfeI GAWTC 2 cut(s) 5, 212
PflFI GACNNNGTC 1 cut(s) 122
PkrI GCNGC 4 cut(s) 34, 37, 60, 451
PleI GAGTC 2 cut(s) 227, 612
PpsI GAGTC 2 cut(s) 227, 612
Ppu21I YACGTR 1 cut(s) 326
PscI ACATGT 1 cut(s) 261
Psp124BI GAGCTC 1 cut(s) 77
Psp6I CCWGG 2 cut(s) 189, 393
PspGI CCWGG 2 cut(s) 189, 393
PspN4I GGNNCC 1 cut(s) 564
PspPI GGNCC 2 cut(s) 18, 124
PstNI CAGNNNCTG 1 cut(s) 455
PsuI RGATCY 2 cut(s) 373, 664
PsyI GACNNNGTC 1 cut(s) 122
SacI GAGCTC 1 cut(s) 77
SapI GCTCTTC 1 cut(s) 606
SaqAI TTAA 3 cut(s) 246, 266, 686
SatI GCNGC 4 cut(s) 33, 36, 59, 450
Sau3AI GATC 6 cut(s) 85, 373, 422, 457, 592, 664
Sau96I GGNCC 2 cut(s) 18, 124
SchI GAGTC 2 cut(s) 228, 613
ScrFI CCNGG 3 cut(s) 191, 371, 395
SduI GDGCHC 4 cut(s) 77, 146, 204, 294
SfaNI GCATC 1 cut(s) 503
SinI GGWCC 2 cut(s) 18, 124
SmlI CTYRAG 1 cut(s) 601
SmoI CTYRAG 1 cut(s) 601
SnaBI TACGTA 1 cut(s) 326
Sse9I AATT 3 cut(s) 243, 484, 510
SsiI CCGC 3 cut(s) 25, 33, 616
SspMI CTAG 1 cut(s) 216
SstI GAGCTC 1 cut(s) 77
StyD4I CCNGG 3 cut(s) 189, 369, 393
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 2 cut(s) 226, 357
TaiI ACGT 4 cut(s) 46, 56, 316, 328
TaqI TCGA 3 cut(s) 108, 165, 421
TasI AATT 3 cut(s) 243, 484, 510
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 2 cut(s) 5, 212
Tru1I TTAA 3 cut(s) 246, 266, 686
Tru9I TTAA 3 cut(s) 246, 266, 686
TscAI CASTG 2 cut(s) 285, 360
TseFI GTSAC 3 cut(s) 220, 439, 689
TseI GCWGC 3 cut(s) 35, 58, 449
Tsp45I GTSAC 3 cut(s) 220, 439, 689
TspDTI ATGAA 2 cut(s) 522, 624
TspRI CASTG 2 cut(s) 285, 360
Tth111I GACNNNGTC 1 cut(s) 122
VpaK11BI GGWCC 2 cut(s) 18, 124
XagI CCTNNNNNAGG 1 cut(s) 495
XapI RAATTY 2 cut(s) 484, 510
XbaI TCTAGA 1 cut(s) 215
XceI RCATGY 1 cut(s) 265
XcmI CCANNNNNNNNNTGG 1 cut(s) 573
XmiI GTMKAC 1 cut(s) 50
XspI CTAG 1 cut(s) 216
ZraI GACGTC 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.