Rmu_sc0002169.1_g000008

Belongs to the peptidase M24B family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002169.1
OG0011079
Physical Location & Seq
Reverse (-)
30893 .. 37550
6658 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2616 bp
atgcactccctctcatcccatgcgacgcgtcctctctcctcttccttctctcactccacctcctacttccgcttcatctccctctccctcccatttctccactctcccaaatcccaccccaaacttttcactatttccccaaaagcccccactttcacgatccggagcatctccgccaagccctcgtcggagctccgacccaagcgccccaattccgactccgactcaaagctccgagctctccgcgacctcttctccaagcccggcgtcggaatcgacgcttacatcattccctctcaggatgctcaccagagtgagttcattgctgaatgttatatgaggaggacctatatatctggtttcacagggagtgccggcactgctgttgtcactaaggataaagcagctctttggacagatggccgctattttcttcaggctgaaaagcagctgagttcgagttggattctcatgcgggctggtaattttggagtacctacaactattgaatggctgaatgatgttttggcccctggtgggagagttggcattgacccttttctgttttcttccgatgctgcagaagaattgaagcagggcatcgctaacaagaatcatgagttggtttttctgtatgataaaaatcttgtggatgaaatatggaaagaatcaagaccagagcctccaaaaaaaccaattagagtacacgaactgaaatatgctggtgcagatgtggcatcaaagttatcatctctgaggtctgaacttgttgcagctggttcatctgcaatagttatatccatgcttgacgaagttgcctggctgttgaatttgagaggaagtgatgttccacattcacctgttatgtatgcttatctgattgtggagatcgacaaagcaaagttatttgtagatacttccaaagtaggcactgaggtgttggatcacctggagaatgcgggtattgaattgaggccatatgactccattctttctgaagtagaaagtctggcagcaaaaggggctaaagtttggttggatgcatcatctgtgaatgcagctattgtccacacctacaacactgcttgtgagaaatattatgagagccttgaaagtaaaagaaaaagtaagactaaggaatataatgactcacatggttggtctgaaggacccactggaatgtatagggtgtcccctgtctctttggcaaaggcactgaaaaatgatgctgagttagaagggatgcggagttgtcatttaagggatgctgctgctttagcgcagttttgggtttggatcgaagagcaaatcaacaagaatgtgaaactaactgagggtcatatagctcttgatcaggcagtattccctgaaaacacaccaggttttgtcttagatgcattcgctcgttcttttctttggaagattggactggattaccgacatggaactgggcatggtgtgggagctgcattaaatgttcatgagggtcctcaaagtattagctttcgatttggaaatacaacttccttacagagtggtatgattgttagcaatgaacctggctattatgaagaccatgccttcggcattcggattgagaatctccttgttgtgaaagaagttgacacgccaaatcgttttggaggaattggatacctgggatttgaaaaactcacatatgttcccatccaggctaaattgatagacttgtctttgctttctggtgcggaatttgattggcttaatgattatcattcacaagtttgggacaaggtctcaccattgcttgatggtcctgcacgccaatggctttggaacaacacaagaccacttgtcaagcaaaccatttcgaaggacaatactaaagaagaccttgtcacagcaatgtcaaagaacaagaataaaggcatcaaggaagatcttgccacaagagtgtcgaagatgaagggtttggtggctcaggatctgagcaaactggatctaactcagttagatccactctcgccggaagttatatctcgtcaggccacgatcaacattggcacaataggtcacgtggctcatggtaagtcaacggtggtcaaagcaatttcgggggttcagacagttcgtttcaaacaggagctggagcgcaacataactataaaaacaggatatgctaatgccaaaatttacaagtgcggtagctgcccccgacccgactgctacaagtcttatggcagtggaaaggaagacaatcctctctgtgaacggtgtgggagtcagatgagactggagagacatgtgtcctttgtagattgccccggtcacgaagcactaatggctactatgcttaatggtgcgtccattatggatggagcgttacttgttgtagctgctgacgaacagtttccccaagaacaaaccaaggagcatctggaagctgttgaaatcatgtctcttaaacacaacattgtgcttcaaaacaaagttgaccttgtccgagagaaggcaatcgagcagcgagaggagatgaaggagtggtttcgtggcacggtagctgaagatgcaacaattgtgcctattgttgctcagctgaagtataatatcgatgtagtgaacgagtacattgtctga

Protein Analysis

871

Amino Acids

96.77

Weight (kDa)

6.52

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Creatinase_N PF01321 80 - 204 1.1e-13 Creatinase/Prolidase N-terminal domain
Creatinase_N_2 PF16189 225 - 411 4.4e-46 Creatinase/Prolidase N-terminal domain
Peptidase_M24 PF00557 444 - 540 2.1e-20 Metallopeptidase family M24
Peptidase_M24_C PF16188 551 - 611 8e-25 C-terminal region of peptidase_M24
GTP_EFTU PF00009 677 - 866 1.9e-24 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 28, 246
AccIII TCCGGA 1 cut(s) 162
AciI CCGC 9 cut(s) 70, 174, 244, 424, 475, 959, 1246, 1727, 2181
AclWI GGATC 6 cut(s) 154, 951, 1304, 1971, 1986, 1988
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 3 cut(s) 829, 1730, 2169
AcuI CTGAAG 5 cut(s) 419, 1017, 1185, 2562, 2597
AcvI CACGTG 1 cut(s) 2056
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 3 cut(s) 495, 705, 2606
AfiI CCNNNNNNNGG 3 cut(s) 269, 537, 2488
AflIII ACRYGT 2 cut(s) 26, 2281
AgsI TTSAA 9 cut(s) 509, 592, 829, 968, 1112, 1667, 2116, 2429, 2462
AhdI GACNNNNNGTC 1 cut(s) 1832
AjnI CCWGG 7 cut(s) 532, 818, 948, 1378, 1558, 1656, 1689
AjuI GAANNNNNNNTTGG 4 cut(s) 509, 541, 605, 637
AleI CACNNNNGTG 3 cut(s) 312, 935, 1931
AloI GAACNNNNNNTCC 2 cut(s) 831, 863
Alw21I GWGCWC 2 cut(s) 195, 241
Alw26I GTCTC 5 cut(s) 1204, 1780, 2263, 2272, 2442
AlwI GGATC 6 cut(s) 154, 951, 1304, 1971, 1986, 1988
AlwNI CAGNNNCTG 2 cut(s) 1966, 2125
Aor13HI TCCGGA 1 cut(s) 162
AoxI GGCC 4 cut(s) 421, 528, 974, 2025
ApoI RAATTY 3 cut(s) 829, 1730, 2169
ArsI GACNNNNNNTTYG 2 cut(s) 1565, 1597
Asp700I GAANNNNTTC 1 cut(s) 2388
AspLEI GCGC 3 cut(s) 207, 1282, 2133
AspS9I GGNCC 5 cut(s) 345, 529, 1169, 1487, 1793
AsuC2I CCSGG 2 cut(s) 264, 2304
AsuHPI GGTGA 4 cut(s) 299, 849, 938, 1770
AsuII TTCGAA 1 cut(s) 1850
AvaII GGWCC 4 cut(s) 345, 1169, 1487, 1793
BanII GRGCYC 2 cut(s) 195, 241
BbrPI CACGTG 1 cut(s) 2056
BbsI GAAGAC 3 cut(s) 1578, 1875, 2238
Bbv12I GWGCWC 2 cut(s) 195, 241
BccI CCATC 4 cut(s) 413, 1694, 1784, 2348
BcgI CGANNNNNNTGC 2 cut(s) 2184, 2218
BciT130I CCWGG 7 cut(s) 534, 820, 950, 1380, 1560, 1658, 1691
BciVI GTATCC 1 cut(s) 1646
BclI TGATCA 1 cut(s) 1351
BcnI CCSGG 2 cut(s) 264, 2304
BcoDI GTCTC 5 cut(s) 1204, 1780, 2263, 2272, 2442
BfmI CTRYAG 1 cut(s) 579
BfoI RGCGCY 1 cut(s) 208
BfuI GTATCC 1 cut(s) 1646
BglII AGATCT 1 cut(s) 1918
BlpI GCTNAGC 1 cut(s) 2571
Bme1390I CCNGG 9 cut(s) 264, 534, 820, 950, 1380, 1560, 1658, 1691, 2304
Bme18I GGWCC 4 cut(s) 345, 1169, 1487, 1793
BmeRI GACNNNNNGTC 1 cut(s) 1832
BmgT120I GGNCC 5 cut(s) 345, 529, 1169, 1487, 1793
BmiI GGNNCC 3 cut(s) 531, 1171, 1488
BmrFI CCNGG 9 cut(s) 264, 534, 820, 950, 1380, 1560, 1658, 1691, 2304
BmrI ACTGGG 1 cut(s) 1458
BmuI ACTGGG 1 cut(s) 1458
BoxI GACNNNNGTC 1 cut(s) 2284
BpiI GAAGAC 3 cut(s) 1578, 1875, 2238
BpmI CTGGAG 3 cut(s) 971, 2147, 2294
Bpu10I CCTNAGC 1 cut(s) 1959
Bpu1102I GCTNAGC 1 cut(s) 2571
Bpu14I TTCGAA 1 cut(s) 1850
BpuMI CCSGG 2 cut(s) 264, 2304
Bsa29I ATCGAT 1 cut(s) 2589
BsaAI YACGTR 1 cut(s) 2056
BsaBI GATNNNNATC 1 cut(s) 642
BsaHI GRCGYC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 1780
BsaJI CCNNGG 4 cut(s) 532, 1657, 2302, 2406
BsaWI WCCGGW 1 cut(s) 162
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 239, 269
Bsc4I CCNNNNNNNGG 3 cut(s) 269, 537, 2488
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 5 cut(s) 1180, 1434, 1453, 1980, 2277
Bse3DI GCAATG 4 cut(s) 321, 1558, 1781, 1890
Bse8I GATNNNNATC 1 cut(s) 642
BseAI TCCGGA 1 cut(s) 162
BseBI CCWGG 7 cut(s) 534, 820, 950, 1380, 1560, 1658, 1691
BseCI ATCGAT 1 cut(s) 2589
BseDI CCNNGG 4 cut(s) 532, 1657, 2302, 2406
BseGI GGATG 8 cut(s) 14, 307, 658, 1045, 1248, 1270, 1686, 2359
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 3 cut(s) 269, 537, 2488
BseMI GCAATG 4 cut(s) 321, 1558, 1781, 1890
BseNI ACTGG 5 cut(s) 1180, 1434, 1453, 1980, 2277
BseRI GAGGAG 3 cut(s) 28, 355, 2522
BsgI GTGCAG 2 cut(s) 747, 1782
Bsh1236I CGCG 2 cut(s) 28, 246
BshFI GGCC 4 cut(s) 423, 530, 976, 2027
BshVI ATCGAT 1 cut(s) 2589
BsiHKAI GWGCWC 2 cut(s) 195, 241
BsiSI CCGG 5 cut(s) 163, 264, 375, 2006, 2304
BslFI GGGAC 2 cut(s) 1177, 1781
BslI CCNNNNNNNGG 3 cut(s) 269, 537, 2488
BsmAI GTCTC 5 cut(s) 1204, 1780, 2263, 2272, 2442
BsmFI GGGAC 2 cut(s) 1177, 1781
BsmI GAATGC 4 cut(s) 961, 1060, 1397, 1587
BsnI GGCC 4 cut(s) 423, 530, 976, 2027
Bso31I GGTCTC 1 cut(s) 1780
Bsp119I TTCGAA 1 cut(s) 1850
Bsp1286I GDGCHC 2 cut(s) 195, 241
Bsp13I TCCGGA 1 cut(s) 162
Bsp1720I GCTNAGC 1 cut(s) 2571
BspACI CCGC 9 cut(s) 70, 174, 244, 424, 475, 959, 1246, 1727, 2181
BspANI GGCC 4 cut(s) 423, 530, 976, 2027
BspDI ATCGAT 1 cut(s) 2589
BspEI TCCGGA 1 cut(s) 162
BspFNI CGCG 2 cut(s) 28, 246
BspHI TCATGA 2 cut(s) 616, 1480
BspLI GGNNCC 3 cut(s) 531, 1171, 1488
BspMAI CTGCAG 1 cut(s) 583
BspPI GGATC 6 cut(s) 154, 951, 1304, 1971, 1986, 1988
BspQI GCTCTTC 1 cut(s) 1296
BspT104I TTCGAA 1 cut(s) 1850
BspTNI GGTCTC 1 cut(s) 1780
BsrDI GCAATG 4 cut(s) 321, 1558, 1781, 1890
BsrFI RCCGGY 1 cut(s) 374
BsrI ACTGG 5 cut(s) 1180, 1434, 1453, 1980, 2277
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 4 cut(s) 532, 1657, 2302, 2406
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 2406
Bst2UI CCWGG 7 cut(s) 534, 820, 950, 1380, 1560, 1658, 1691
Bst4CI ACNGT 5 cut(s) 2077, 2107, 2253, 2388, 2536
Bst6I CTCTTC 3 cut(s) 46, 257, 1296
BstACI GRCGYC 1 cut(s) 267
BstBAI YACGTR 1 cut(s) 2056
BstBI TTCGAA 1 cut(s) 1850
BstC8I GCNNGC 3 cut(s) 376, 477, 1801
BstF5I GGATG 8 cut(s) 14, 307, 658, 1045, 1248, 1270, 1686, 2359
BstFNI CGCG 2 cut(s) 28, 246
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 3 cut(s) 207, 1282, 2133
BstMAI GTCTC 5 cut(s) 1204, 1780, 2263, 2272, 2442
BstMWI GCNNNNNNNGC 7 cut(s) 380, 734, 1022, 1277, 2187, 2321, 2546
BstNI CCWGG 7 cut(s) 534, 820, 950, 1380, 1560, 1658, 1691
BstNSI RCATGY 1 cut(s) 2285
BstPAI GACNNNNGTC 1 cut(s) 2284
BstSCI CCNGG 9 cut(s) 262, 532, 818, 948, 1378, 1558, 1656, 1689, 2302
BstSFI CTRYAG 1 cut(s) 579
BstUI CGCG 2 cut(s) 28, 246
BstV2I GAAGAC 3 cut(s) 1578, 1875, 2238
BstX2I RGATCY 4 cut(s) 1918, 1963, 1978, 1993
BstYI RGATCY 4 cut(s) 1918, 1963, 1978, 1993
Bsu15I ATCGAT 1 cut(s) 2589
BsuI GTATCC 1 cut(s) 1646
BsuRI GGCC 4 cut(s) 423, 530, 976, 2027
BsuTUI ATCGAT 1 cut(s) 2589
BtgZI GCGATG 1 cut(s) 586
BtsCI GGATG 8 cut(s) 14, 307, 658, 1045, 1248, 1270, 1686, 2359
BtsI GCAGTG 3 cut(s) 378, 1080, 2227
BtsIMutI CAGTG 6 cut(s) 378, 930, 1080, 1173, 1214, 2227
Cac8I GCNNGC 3 cut(s) 376, 477, 1801
CaiI CAGNNNCTG 2 cut(s) 1966, 2125
CciI TCATGA 2 cut(s) 616, 1480
CfoI GCGC 3 cut(s) 207, 1282, 2133
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 5 cut(s) 345, 529, 1169, 1487, 1793
ClaI ATCGAT 1 cut(s) 2589
CseI GACGC 5 cut(s) 17, 34, 256, 287, 2331
CsiI ACCWGGT 1 cut(s) 1378
Csp6I GTAC 3 cut(s) 494, 704, 2605
CviQI GTAC 3 cut(s) 494, 704, 2605
DriI GACNNNNNGTC 1 cut(s) 1832
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 3 cut(s) 46, 257, 1296
Eam1105I GACNNNNNGTC 1 cut(s) 1832
EarI CTCTTC 3 cut(s) 46, 257, 1296
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 2 cut(s) 193, 239
Eco130I CCWWGG 1 cut(s) 2406
Eco24I GRGCYC 2 cut(s) 195, 241
Eco31I GGTCTC 1 cut(s) 1780
Eco47I GGWCC 4 cut(s) 345, 1169, 1487, 1793
Eco53kI GAGCTC 2 cut(s) 193, 239
Eco57I CTGAAG 5 cut(s) 419, 1017, 1185, 2562, 2597
Eco72I CACGTG 1 cut(s) 2056
EcoICRI GAGCTC 2 cut(s) 193, 239
EcoO109I RGGNCCY 3 cut(s) 345, 1169, 1487
EcoRII CCWGG 7 cut(s) 532, 818, 948, 1378, 1558, 1656, 1689
EcoT14I CCWWGG 1 cut(s) 2406
EcoT22I ATGCAT 2 cut(s) 1045, 1399
EcoT38I GRGCYC 2 cut(s) 195, 241
ErhI CCWWGG 1 cut(s) 2406
FalI AAGNNNNNCTT 6 cut(s) 393, 425, 1857, 1889, 2460, 2492
FaqI GGGAC 2 cut(s) 1177, 1781
FauI CCCGC 2 cut(s) 468, 952
FauNDI CATATG 2 cut(s) 979, 1678
FbaI TGATCA 1 cut(s) 1351
FokI GGATG 7 cut(s) 314, 665, 1052, 1255, 1277, 1673, 2366
FriOI GRGCYC 2 cut(s) 195, 241
GlaI GCGC 3 cut(s) 206, 1281, 2132
GsuI CTGGAG 3 cut(s) 971, 2147, 2294
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 4 cut(s) 423, 530, 976, 2027
HapII CCGG 5 cut(s) 163, 264, 375, 2006, 2304
HgaI GACGC 5 cut(s) 17, 34, 256, 287, 2331
HhaI GCGC 3 cut(s) 207, 1282, 2133
Hin1I GRCGYC 1 cut(s) 267
Hin6I GCGC 3 cut(s) 205, 1280, 2131
HinP1I GCGC 3 cut(s) 205, 1280, 2131
HincII GTYRAC 3 cut(s) 1624, 2073, 2473
HindII GTYRAC 3 cut(s) 1624, 2073, 2473
HpaII CCGG 5 cut(s) 163, 264, 375, 2006, 2304
HphI GGTGA 4 cut(s) 299, 849, 938, 1770
Hpy166II GTNNAC 7 cut(s) 706, 1069, 1624, 2073, 2249, 2473, 2599
Hpy8I GTNNAC 7 cut(s) 706, 1069, 1624, 2073, 2249, 2473, 2599
Hpy99I CGWCG 4 cut(s) 28, 190, 272, 281
HpyAV CCTTC 8 cut(s) 55, 1160, 1232, 1591, 1846, 1939, 2482, 2509
HpyCH4III ACNGT 5 cut(s) 2077, 2107, 2253, 2388, 2536
HpyCH4IV ACGT 1 cut(s) 2055
HpyF10VI GCNNNNNNNGC 7 cut(s) 380, 734, 1022, 1277, 2187, 2321, 2546
HpySE526I ACGT 1 cut(s) 2055
Hsp92I GRCGYC 1 cut(s) 267
HspAI GCGC 3 cut(s) 205, 1280, 2131
Kpn2I TCCGGA 1 cut(s) 162
KroI GCCGGC 1 cut(s) 374
KroNI GCCGGC 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 1351
LguI GCTCTTC 1 cut(s) 1296
LmnI GCTCC 9 cut(s) 165, 190, 198, 237, 1463, 2122, 2128, 2357, 2410
MabI ACCWGGT 1 cut(s) 1378
MaeII ACGT 1 cut(s) 2055
MaeIII GTNAC 5 cut(s) 388, 1876, 2051, 2306, 2361
MfeI CAATTG 1 cut(s) 2553
MflI RGATCY 4 cut(s) 1918, 1963, 1978, 1993
MhlI GDGCHC 2 cut(s) 195, 241
MluI ACGCGT 1 cut(s) 26
MlyI GAGTC 5 cut(s) 212, 218, 977, 1142, 2269
MmeI TCCRAC 8 cut(s) 168, 220, 240, 246, 250, 443, 921, 1017
Mph1103I ATGCAT 2 cut(s) 1045, 1399
MroI TCCGGA 1 cut(s) 162
MroNI GCCGGC 1 cut(s) 374
MroXI GAANNNNTTC 1 cut(s) 2388
MseI TTAA 5 cut(s) 1259, 1472, 1743, 2334, 2442
MslI CAYNNNNRTG 6 cut(s) 312, 935, 1178, 1804, 1883, 1931
MspA1I CMGCKG 3 cut(s) 451, 776, 2575
MspI CCGG 5 cut(s) 163, 264, 375, 2006, 2304
MspR9I CCNGG 9 cut(s) 264, 534, 820, 950, 1380, 1560, 1658, 1691, 2304
MunI CAATTG 1 cut(s) 2553
Mva1269I GAATGC 4 cut(s) 961, 1060, 1397, 1587
MvaI CCWGG 7 cut(s) 534, 820, 950, 1380, 1560, 1658, 1691
MvnI CGCG 2 cut(s) 28, 246
MwoI GCNNNNNNNGC 7 cut(s) 380, 734, 1022, 1277, 2187, 2321, 2546
NaeI GCCGGC 1 cut(s) 376
NciI CCSGG 2 cut(s) 264, 2304
NdeI CATATG 2 cut(s) 979, 1678
NgoMIV GCCGGC 1 cut(s) 374
NlaIV GGNNCC 3 cut(s) 531, 1171, 1488
NmuCI GTSAC 4 cut(s) 388, 1876, 2051, 2306
NsiI ATGCAT 2 cut(s) 1045, 1399
NspI RCATGY 1 cut(s) 2285
NspV TTCGAA 1 cut(s) 1850
OliI CACNNNNGTG 3 cut(s) 312, 935, 1931
PagI TCATGA 2 cut(s) 616, 1480
PciI ACATGT 1 cut(s) 2281
PciSI GCTCTTC 1 cut(s) 1296
PctI GAATGC 4 cut(s) 961, 1060, 1397, 1587
PdiI GCCGGC 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 2388
PfeI GAWTC 5 cut(s) 273, 466, 613, 668, 1600
PflFI GACNNNGTC 3 cut(s) 1772, 1874, 2477
PleI GAGTC 5 cut(s) 212, 218, 977, 1142, 2268
PmaCI CACGTG 1 cut(s) 2056
PmlI CACGTG 1 cut(s) 2056
PpsI GAGTC 5 cut(s) 212, 218, 977, 1142, 2268
Ppu21I YACGTR 1 cut(s) 2056
PpuMI RGGWCCY 3 cut(s) 345, 1169, 1487
PscI ACATGT 1 cut(s) 2281
PshAI GACNNNNGTC 1 cut(s) 2284
Psp124BI GAGCTC 2 cut(s) 195, 241
Psp5II RGGWCCY 3 cut(s) 345, 1169, 1487
Psp6I CCWGG 7 cut(s) 532, 818, 948, 1378, 1558, 1656, 1689
PspCI CACGTG 1 cut(s) 2056
PspGI CCWGG 7 cut(s) 532, 818, 948, 1378, 1558, 1656, 1689
PspN4I GGNNCC 3 cut(s) 531, 1171, 1488
PspPI GGNCC 5 cut(s) 345, 529, 1169, 1487, 1793
PspPPI RGGWCCY 3 cut(s) 345, 1169, 1487
PstI CTGCAG 1 cut(s) 583
PstNI CAGNNNCTG 2 cut(s) 1966, 2125
PsuI RGATCY 4 cut(s) 1918, 1963, 1978, 1993
PsyI GACNNNGTC 3 cut(s) 1772, 1874, 2477
PvuII CAGCTG 3 cut(s) 451, 776, 2575
RsaI GTAC 3 cut(s) 495, 705, 2606
RsaNI GTAC 3 cut(s) 494, 704, 2605
RseI CAYNNNNRTG 6 cut(s) 312, 935, 1178, 1804, 1883, 1931
SacI GAGCTC 2 cut(s) 195, 241
SapI GCTCTTC 1 cut(s) 1296
SaqAI TTAA 5 cut(s) 1259, 1472, 1743, 2334, 2442
Sau96I GGNCC 5 cut(s) 345, 529, 1169, 1487, 1793
SchI GAGTC 5 cut(s) 212, 218, 977, 1142, 2269
ScrFI CCNGG 9 cut(s) 264, 534, 820, 950, 1380, 1560, 1658, 1691, 2304
SduI GDGCHC 2 cut(s) 195, 241
SexAI ACCWGGT 1 cut(s) 1378
SfcI CTRYAG 1 cut(s) 579
SfuI TTCGAA 1 cut(s) 1850
SinI GGWCC 4 cut(s) 345, 1169, 1487, 1793
SmiMI CAYNNNNRTG 6 cut(s) 312, 935, 1178, 1804, 1883, 1931
SsiI CCGC 9 cut(s) 70, 174, 244, 424, 475, 959, 1246, 1727, 2181
SspI AATATT 1 cut(s) 1097
SstI GAGCTC 2 cut(s) 195, 241
StyD4I CCNGG 9 cut(s) 262, 532, 818, 948, 1378, 1558, 1656, 1689, 2302
StyI CCWWGG 1 cut(s) 2406
TaaI ACNGT 5 cut(s) 2077, 2107, 2253, 2388, 2536
TaiI ACGT 1 cut(s) 2058
TaqI TCGA 9 cut(s) 276, 458, 891, 1299, 1507, 1850, 1937, 2496, 2589
TatI WGTACW 2 cut(s) 703, 2604
TauI GCSGC 1 cut(s) 426
TfiI GAWTC 5 cut(s) 273, 466, 613, 668, 1600
Tru1I TTAA 5 cut(s) 1259, 1472, 1743, 2334, 2442
Tru9I TTAA 5 cut(s) 1259, 1472, 1743, 2334, 2442
TscAI CASTG 6 cut(s) 385, 937, 1087, 1180, 1221, 2227
TseFI GTSAC 4 cut(s) 388, 1876, 2051, 2306
Tsp45I GTSAC 4 cut(s) 388, 1876, 2051, 2306
TspDTI ATGAA 9 cut(s) 64, 310, 669, 771, 1469, 1569, 1584, 1958, 2528
TspRI CASTG 6 cut(s) 385, 937, 1087, 1180, 1221, 2227
Tth111I GACNNNGTC 3 cut(s) 1772, 1874, 2477
VpaK11BI GGWCC 4 cut(s) 345, 1169, 1487, 1793
XapI RAATTY 3 cut(s) 829, 1730, 2169
XceI RCATGY 1 cut(s) 2285
XcmI CCANNNNNNNNNTGG 1 cut(s) 2413
XmnI GAANNNNTTC 1 cut(s) 2388
Zsp2I ATGCAT 2 cut(s) 1045, 1399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.