Rmu_sc0002183.1_g000014
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002183.1
OG0018676
Physical Location & Seq
Reverse (-)
61815 .. 63498
1684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002183.1_g000014.1.cds

Sequence Viewer

Length: 1245 bp
atgagaaacaaagttgaaaaagaagagctgaactgtgaaggtcggttgtggctcattagatatatcgatggatcattccctcgtccaccacaacacattattacagaagaaggtggggttatgtctgatatgacaaaggagtacatggactgggtgcaaaatgattctgccgttatgtctatacttgcagctactctgtctaatgatgttctttcctttattgttggaagtaagacttctagggaaatttggtgtcgacttaaggagaaatatgctagtgcatcggcatataacataatgacattgaagatctccttatacaacatccaaaaaggctcagactctattgacatgtatttacttcgagttaagttactatgtgatcagcttgctattgtaggaatttatctggcagatgaggatataatatatagtctcaatgagattaatcaaactgtgatgtccatgtctttgtcctccaagactgctatggctgcatttggtgatacttccagagctcaagctactaatgctgcatgtgtctctagtgatatgatgtttacaaggtatagctctgatgataactcaacttctgattccactgtggctgaaagtcagagtgttgatcaactgattacagaagattgtgcttatgaagacattcaaattcctcaagattcgaagaagaaacaagttggagaagactcttattcagataaagaggagcaattgaactctcaagaacatgtttttctggatgagaaagttgcttctaataaagataagtttctacaagaaaagaaatctacttcttttgttgtaatagaatctacttctgattctgtttcaggttatttttgttcgatccctgagctttcctttatgagtcctcttatgagttctcttggcaacaaaagtgataatgcaaccaactcaagaacagaagctcaggcacattttatgacatctgagcaggttcaagagtacaatcaaggaaagcaaacatttgttgcaaagtcaacaaggagtgaaaagcaaaggaatgatgaattgccattttacaaatgcaaaaatatcgaatctggtttatctcttgataatgctttcaggagggaatatcaccaagattttcatgatcaaggtgtaagaggtgaacaacacgatgattatgattacaagcctcaatctctacttggagcttatctgatacaaggctcttggcagctgttatccactccttcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

414

Amino Acids

46.77

Weight (kDa)

4.75

Isoelectric Point (pI)

55.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 964
AccI GTMKAC 1 cut(s) 256
AclWI GGATC 2 cut(s) 79, 859
AcsI RAATTY 3 cut(s) 246, 402, 666
AfaI GTAC 2 cut(s) 143, 986
AflII CTTAAG 1 cut(s) 260
AflIII ACRYGT 2 cut(s) 351, 745
AgsI TTSAA 5 cut(s) 17, 307, 665, 733, 980
Alw21I GWGCWC 1 cut(s) 520
Alw26I GTCTC 2 cut(s) 440, 547
AlwI GGATC 2 cut(s) 79, 859
ApeKI GCWGC 4 cut(s) 188, 494, 533, 1222
ApoI RAATTY 3 cut(s) 246, 402, 666
AseI ATTAAT 1 cut(s) 447
Asp700I GAANNNNTTC 1 cut(s) 660
AsuHPI GGTGA 3 cut(s) 515, 1112, 1163
AsuII TTCGAA 1 cut(s) 680
BanII GRGCYC 1 cut(s) 520
BbsI GAAGAC 2 cut(s) 663, 708
Bbv12I GWGCWC 1 cut(s) 520
BbvI GCAGC 4 cut(s) 200, 481, 520, 1234
BccI CCATC 1 cut(s) 62
BceAI ACGGC 1 cut(s) 155
BcgI CGANNNNNNTGC 2 cut(s) 1057, 1091
BclI TGATCA 3 cut(s) 382, 625, 1135
BcoDI GTCTC 2 cut(s) 440, 547
BfaI CTAG 3 cut(s) 240, 276, 546
BfrI CTTAAG 1 cut(s) 260
BfuAI ACCTGC 1 cut(s) 964
BglII AGATCT 1 cut(s) 309
BisI GCNGC 4 cut(s) 189, 495, 534, 1223
BlsI GCNGC 4 cut(s) 190, 496, 535, 1224
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 1 cut(s) 290
BmuI ACTGGG 1 cut(s) 160
BpiI GAAGAC 2 cut(s) 663, 708
Bpu10I CCTNAGC 2 cut(s) 870, 948
Bpu14I TTCGAA 1 cut(s) 680
BpuEI CTTGAG 4 cut(s) 504, 657, 723, 919
Bsa29I ATCGAT 1 cut(s) 66
Bse1I ACTGG 1 cut(s) 155
BseCI ATCGAT 1 cut(s) 66
BseGI GGATG 2 cut(s) 324, 763
BseMII CTCAG 4 cut(s) 351, 861, 960, 962
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 1 cut(s) 737
BseXI GCAGC 4 cut(s) 200, 481, 520, 1234
BshVI ATCGAT 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 520
BsmAI GTCTC 2 cut(s) 440, 547
Bsp119I TTCGAA 1 cut(s) 680
Bsp1286I GDGCHC 1 cut(s) 520
Bsp143I GATC 6 cut(s) 71, 309, 382, 625, 864, 1135
BspCNI CTCAG 4 cut(s) 350, 862, 961, 961
BspDI ATCGAT 1 cut(s) 66
BspHI TCATGA 1 cut(s) 1132
BspMI ACCTGC 1 cut(s) 964
BspPI GGATC 2 cut(s) 79, 859
BspQI GCTCTTC 1 cut(s) 18
BspT104I TTCGAA 1 cut(s) 680
BspTI CTTAAG 1 cut(s) 260
BsrI ACTGG 1 cut(s) 155
BssMI GATC 6 cut(s) 71, 309, 382, 625, 864, 1135
Bst4CI ACNGT 3 cut(s) 35, 457, 604
Bst6I CTCTTC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 260
BstBI TTCGAA 1 cut(s) 680
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 4 cut(s) 337, 870, 948, 969
BstF5I GGATG 2 cut(s) 324, 763
BstKTI GATC 6 cut(s) 74, 312, 385, 628, 867, 1138
BstMAI GTCTC 2 cut(s) 440, 547
BstMBI GATC 6 cut(s) 71, 309, 382, 625, 864, 1135
BstMWI GCNNNNNNNGC 2 cut(s) 494, 530
BstNSI RCATGY 3 cut(s) 355, 540, 749
BstV1I GCAGC 4 cut(s) 200, 481, 520, 1234
BstV2I GAAGAC 2 cut(s) 663, 708
BstX2I RGATCY 1 cut(s) 309
BstYI RGATCY 1 cut(s) 309
Bsu15I ATCGAT 1 cut(s) 66
BsuTUI ATCGAT 1 cut(s) 66
BtsCI GGATG 2 cut(s) 324, 763
BtsIMutI CAGTG 1 cut(s) 600
BveI ACCTGC 1 cut(s) 964
Cac8I GCNNGC 1 cut(s) 390
CciI TCATGA 1 cut(s) 1132
ClaI ATCGAT 1 cut(s) 66
Csp6I GTAC 2 cut(s) 142, 985
CviAII CATG 6 cut(s) 145, 352, 466, 537, 746, 1133
CviQI GTAC 2 cut(s) 142, 985
DdeI CTNAG 4 cut(s) 337, 870, 948, 969
DpnI GATC 6 cut(s) 73, 311, 384, 627, 866, 1137
DpnII GATC 6 cut(s) 71, 309, 382, 625, 864, 1135
Eam1104I CTCTTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 18
Ecl136II GAGCTC 1 cut(s) 518
Eco24I GRGCYC 1 cut(s) 520
Eco53kI GAGCTC 1 cut(s) 518
EcoICRI GAGCTC 1 cut(s) 518
EcoT38I GRGCYC 1 cut(s) 520
FaeI CATG 6 cut(s) 148, 355, 469, 540, 749, 1136
FalI AAGNNNNNCTT 4 cut(s) 220, 252, 299, 331
FatI CATG 6 cut(s) 144, 351, 465, 536, 745, 1132
FbaI TGATCA 3 cut(s) 382, 625, 1135
FblI GTMKAC 1 cut(s) 256
Fnu4HI GCNGC 4 cut(s) 189, 495, 534, 1223
FokI GGATG 2 cut(s) 311, 770
FriOI GRGCYC 1 cut(s) 520
Fsp4HI GCNGC 4 cut(s) 189, 495, 534, 1223
FspBI CTAG 3 cut(s) 240, 276, 546
GluI GCNGC 4 cut(s) 189, 495, 534, 1223
Hin1II CATG 6 cut(s) 148, 355, 469, 540, 749, 1136
HincII GTYRAC 2 cut(s) 257, 1020
HindII GTYRAC 2 cut(s) 257, 1020
HinfI GANTC 9 cut(s) 164, 341, 596, 677, 704, 827, 839, 886, 1079
HphI GGTGA 3 cut(s) 515, 1112, 1163
Hpy166II GTNNAC 5 cut(s) 86, 257, 561, 1020, 1154
Hpy188I TCNGA 9 cut(s) 127, 340, 577, 595, 618, 715, 838, 970, 1206
Hpy188III TCNNGA 9 cut(s) 513, 674, 740, 755, 936, 980, 1094, 1108, 1133
Hpy8I GTNNAC 5 cut(s) 86, 257, 561, 1020, 1154
HpyAV CCTTC 2 cut(s) 32, 104
HpyCH4III ACNGT 3 cut(s) 35, 457, 604
HpyCH4V TGCA 8 cut(s) 157, 188, 281, 497, 536, 926, 1013, 1068
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 530
HpyF3I CTNAG 4 cut(s) 337, 870, 948, 969
Hsp92II CATG 6 cut(s) 148, 355, 469, 540, 749, 1136
Ksp22I TGATCA 3 cut(s) 382, 625, 1135
Kzo9I GATC 6 cut(s) 71, 309, 382, 625, 864, 1135
LguI GCTCTTC 1 cut(s) 18
LmnI GCTCC 2 cut(s) 724, 1196
Lsp1109I GCAGC 4 cut(s) 200, 481, 520, 1234
LweI GCATC 1 cut(s) 290
MaeI CTAG 3 cut(s) 240, 276, 546
MaeIII GTNAC 1 cut(s) 372
MalI GATC 6 cut(s) 73, 311, 384, 627, 866, 1137
MboI GATC 6 cut(s) 71, 309, 382, 625, 864, 1135
MboII GAAGA 8 cut(s) 35, 119, 319, 653, 668, 694, 697, 713
MfeI CAATTG 1 cut(s) 728
MflI RGATCY 1 cut(s) 309
MhlI GDGCHC 1 cut(s) 520
MluCI AATT 5 cut(s) 246, 402, 666, 728, 1049
MlyI GAGTC 3 cut(s) 335, 698, 895
MmeI TCCRAC 2 cut(s) 205, 676
MnlI CCTC 9 cut(s) 90, 412, 487, 681, 715, 900, 1104, 1142, 1191
MroXI GAANNNNTTC 1 cut(s) 660
MseI TTAA 3 cut(s) 261, 369, 447
MspA1I CMGCKG 1 cut(s) 1225
MspCI CTTAAG 1 cut(s) 260
MunI CAATTG 1 cut(s) 728
MwoI GCNNNNNNNGC 2 cut(s) 494, 530
NdeII GATC 6 cut(s) 71, 309, 382, 625, 864, 1135
NlaIII CATG 6 cut(s) 148, 355, 469, 540, 749, 1136
NspI RCATGY 3 cut(s) 355, 540, 749
NspV TTCGAA 1 cut(s) 680
PagI TCATGA 1 cut(s) 1132
PciI ACATGT 2 cut(s) 351, 745
PciSI GCTCTTC 1 cut(s) 18
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 6 cut(s) 164, 596, 677, 827, 839, 1079
PkrI GCNGC 4 cut(s) 190, 496, 535, 1224
PleI GAGTC 3 cut(s) 335, 698, 894
PpsI GAGTC 3 cut(s) 335, 698, 894
PscI ACATGT 2 cut(s) 351, 745
PshBI ATTAAT 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 520
PsuI RGATCY 1 cut(s) 309
PvuII CAGCTG 1 cut(s) 1225
RsaI GTAC 2 cut(s) 143, 986
RsaNI GTAC 2 cut(s) 142, 985
SacI GAGCTC 1 cut(s) 520
SalI GTCGAC 1 cut(s) 255
SapI GCTCTTC 1 cut(s) 18
SaqAI TTAA 3 cut(s) 261, 369, 447
SatI GCNGC 4 cut(s) 189, 495, 534, 1223
Sau3AI GATC 6 cut(s) 71, 309, 382, 625, 864, 1135
SchI GAGTC 3 cut(s) 335, 698, 895
SduI GDGCHC 1 cut(s) 520
SfaNI GCATC 1 cut(s) 290
SfuI TTCGAA 1 cut(s) 680
SmlI CTYRAG 5 cut(s) 260, 519, 672, 738, 934
SmoI CTYRAG 5 cut(s) 260, 519, 672, 738, 934
Sse9I AATT 5 cut(s) 246, 402, 666, 728, 1049
SspMI CTAG 3 cut(s) 240, 276, 546
SstI GAGCTC 1 cut(s) 520
TaaI ACNGT 3 cut(s) 35, 457, 604
TaqI TCGA 6 cut(s) 66, 256, 364, 680, 863, 1077
TasI AATT 5 cut(s) 246, 402, 666, 728, 1049
TatI WGTACW 2 cut(s) 141, 984
TfiI GAWTC 6 cut(s) 164, 596, 677, 827, 839, 1079
Tru1I TTAA 3 cut(s) 261, 369, 447
Tru9I TTAA 3 cut(s) 261, 369, 447
TscAI CASTG 1 cut(s) 607
TseI GCWGC 4 cut(s) 188, 494, 533, 1222
TspDTI ATGAA 4 cut(s) 669, 1062, 1121, 1230
TspRI CASTG 1 cut(s) 607
Vha464I CTTAAG 1 cut(s) 260
VspI ATTAAT 1 cut(s) 447
XapI RAATTY 3 cut(s) 246, 402, 666
XceI RCATGY 3 cut(s) 355, 540, 749
XcmI CCANNNNNNNNNTGG 1 cut(s) 487
XmiI GTMKAC 1 cut(s) 256
XmnI GAANNNNTTC 1 cut(s) 660
XspI CTAG 3 cut(s) 240, 276, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.