Rroxscaffold_1G00009430

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0018676
Physical Location & Seq
Reverse (-)
11931779 .. 11939921
8143 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00009430.1

Sequence Viewer

Length: 558 bp
ATGTTCTCCAAGCCGTTTCTTCCTCCTCCGACCACCACCGGACGTGAAACTCAAGGGGTAGCATCGCCTCAACCTTGCGCACCTCCCCACGCCGCCTTGCGGCTTATCTCCGCCGGTAGTCGCCACCGCAAGGGAAACCGGTTTGAGTCCGAAATCGAGTCAATGCCGATATCTCACCACGAAGCAGCCAATCTATTCAGTAATGTCATTGTTCTTACCAGCTGGGCGATGGCAACAGACCGAGTAATAGTAGTCCAGGGCGTTCTAAAAAGGACCAGCTTCATGACCGAGTTAACACCTGAGCATCAACGAGATATGACCAGCACCCAAGTGCTGGCCTTTGCCCACCGGCATCGGCGTTGGCATAGGAGAAATGTTATGGGTAAAACTGCTCAAGAGTACAATCAAGGAAAGCAAACATTTGCTGCAAAGTCAACGAGTGAAAAGCAAAGGAATGATGAATTGTCATTTTACAAATGCAAAAATATCGAATCTGGTTTATCTCTTGATAATGCTTTCGGAGAGAATATCACCAAGATTTTCATGATCAAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.93

Weight (kDa)

9.94

Isoelectric Point (pI)

54.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 79
AccB7I CCANNNNNTGG 1 cut(s) 334
AciI CCGC 4 cut(s) 93, 100, 111, 127
AfaI GTAC 1 cut(s) 401
AfiI CCNNNNNNNGG 3 cut(s) 99, 130, 334
AgeI ACCGGT 1 cut(s) 138
AjiI CACGTC 1 cut(s) 44
AjnI CCWGG 1 cut(s) 255
AleI CACNNNNGTG 1 cut(s) 329
AluBI AGCT 2 cut(s) 222, 279
AluI AGCT 2 cut(s) 222, 279
AoxI GGCC 1 cut(s) 336
ApeKI GCWGC 2 cut(s) 185, 425
AsiGI ACCGGT 1 cut(s) 138
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 273
AsuHPI GGTGA 2 cut(s) 167, 523
AvaII GGWCC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 197, 412
BccI CCATC 1 cut(s) 223
BcgI CGANNNNNNTGC 2 cut(s) 469, 503
BciT130I CCWGG 1 cut(s) 257
BclI TGATCA 1 cut(s) 546
BisI GCNGC 4 cut(s) 93, 101, 186, 426
BlsI GCNGC 4 cut(s) 94, 102, 187, 427
Bme1390I CCNGG 1 cut(s) 257
Bme18I GGWCC 1 cut(s) 273
BmgBI CACGTC 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 273
BmrFI CCNGG 1 cut(s) 257
BmsI GCATC 3 cut(s) 71, 313, 361
Bpu10I CCTNAGC 1 cut(s) 300
BpuEI CTTGAG 2 cut(s) 36, 378
BsaJI CCNNGG 1 cut(s) 256
BsaWI WCCGGW 2 cut(s) 38, 138
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 130, 334
Bse118I RCCGGY 3 cut(s) 113, 138, 348
BseBI CCWGG 1 cut(s) 257
BseDI CCNNGG 1 cut(s) 256
BseLI CCNNNNNNNGG 3 cut(s) 99, 130, 334
BseMII CTCAG 1 cut(s) 291
BseRI GAGGAG 1 cut(s) 15
BseXI GCAGC 2 cut(s) 197, 412
BseYI CCCAGC 1 cut(s) 222
BshFI GGCC 1 cut(s) 338
BshTI ACCGGT 1 cut(s) 138
BsiSI CCGG 4 cut(s) 39, 114, 139, 349
BslI CCNNNNNNNGG 3 cut(s) 99, 130, 334
BsnI GGCC 1 cut(s) 338
Bsp143I GATC 1 cut(s) 546
BspACI CCGC 4 cut(s) 93, 100, 111, 127
BspANI GGCC 1 cut(s) 338
BspCNI CTCAG 1 cut(s) 292
BspHI TCATGA 2 cut(s) 282, 543
BsrFI RCCGGY 3 cut(s) 113, 138, 348
BssAI RCCGGY 3 cut(s) 113, 138, 348
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 257
BstC8I GCNNGC 1 cut(s) 336
BstDEI CTNAG 1 cut(s) 300
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 1 cut(s) 549
BstMBI GATC 1 cut(s) 546
BstNI CCWGG 1 cut(s) 257
BstSCI CCNGG 1 cut(s) 255
BstV1I GCAGC 2 cut(s) 197, 412
BsuRI GGCC 1 cut(s) 338
BtgZI GCGATG 2 cut(s) 48, 242
BtrI CACGTC 1 cut(s) 44
Cac8I GCNNGC 1 cut(s) 336
CciI TCATGA 2 cut(s) 282, 543
CfoI GCGC 1 cut(s) 80
Cfr10I RCCGGY 3 cut(s) 113, 138, 348
Cfr13I GGNCC 1 cut(s) 273
Csp6I GTAC 1 cut(s) 400
CspAI ACCGGT 1 cut(s) 138
CviAII CATG 2 cut(s) 283, 544
CviJI RGCY 6 cut(s) 13, 103, 188, 222, 279, 338
CviKI_1 RGCY 6 cut(s) 13, 103, 188, 222, 279, 338
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 1 cut(s) 300
DpnI GATC 1 cut(s) 548
DpnII GATC 1 cut(s) 546
EciI GGCGGA 1 cut(s) 100
Eco32I GATATC 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 255
EcoRV GATATC 1 cut(s) 171
FaeI CATG 2 cut(s) 286, 547
FaiI YATR 5 cut(s) 284, 317, 366, 380, 545
FatI CATG 2 cut(s) 282, 543
FbaI TGATCA 1 cut(s) 546
Fnu4HI GCNGC 4 cut(s) 93, 101, 186, 426
Fsp4HI GCNGC 4 cut(s) 93, 101, 186, 426
FspI TGCGCA 1 cut(s) 79
GlaI GCGC 1 cut(s) 79
GluI GCNGC 4 cut(s) 93, 101, 186, 426
GsaI CCCAGC 1 cut(s) 226
HaeIII GGCC 1 cut(s) 338
HapII CCGG 4 cut(s) 39, 114, 139, 349
HhaI GCGC 1 cut(s) 80
Hin1II CATG 2 cut(s) 286, 547
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 2 cut(s) 294, 435
HindII GTYRAC 2 cut(s) 294, 435
HinfI GANTC 3 cut(s) 146, 158, 491
HpaI GTTAAC 1 cut(s) 294
HpaII CCGG 4 cut(s) 39, 114, 139, 349
HphI GGTGA 2 cut(s) 167, 523
Hpy166II GTNNAC 2 cut(s) 294, 435
Hpy188I TCNGA 3 cut(s) 30, 151, 521
Hpy188III TCNNGA 4 cut(s) 283, 395, 506, 544
Hpy8I GTNNAC 2 cut(s) 294, 435
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 2 cut(s) 428, 480
HpyF3I CTNAG 1 cut(s) 300
HpySE526I ACGT 1 cut(s) 43
Hsp92II CATG 2 cut(s) 286, 547
HspAI GCGC 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 546
KspAI GTTAAC 1 cut(s) 294
Kzo9I GATC 1 cut(s) 546
Lsp1109I GCAGC 2 cut(s) 197, 412
LweI GCATC 3 cut(s) 71, 313, 361
MaeII ACGT 1 cut(s) 43
MalI GATC 1 cut(s) 548
MboI GATC 1 cut(s) 546
MboII GAAGA 1 cut(s) 11
MluCI AATT 1 cut(s) 461
MlyI GAGTC 2 cut(s) 155, 167
MmeI TCCRAC 1 cut(s) 53
MnlI CCTC 4 cut(s) 33, 36, 78, 93
MseI TTAA 1 cut(s) 293
MslI CAYNNNNRTG 1 cut(s) 329
MspA1I CMGCKG 1 cut(s) 222
MspI CCGG 4 cut(s) 39, 114, 139, 349
MspR9I CCNGG 1 cut(s) 257
MvaI CCWGG 1 cut(s) 257
NdeII GATC 1 cut(s) 546
NlaIII CATG 2 cut(s) 286, 547
NsbI TGCGCA 1 cut(s) 79
OliI CACNNNNGTG 1 cut(s) 329
PagI TCATGA 2 cut(s) 282, 543
PfeI GAWTC 1 cut(s) 491
PflMI CCANNNNNTGG 1 cut(s) 334
PinAI ACCGGT 1 cut(s) 138
PkrI GCNGC 4 cut(s) 94, 102, 187, 427
PleI GAGTC 2 cut(s) 154, 166
PpsI GAGTC 2 cut(s) 154, 166
Psp6I CCWGG 1 cut(s) 255
PspFI CCCAGC 1 cut(s) 222
PspGI CCWGG 1 cut(s) 255
PspPI GGNCC 1 cut(s) 273
PvuII CAGCTG 1 cut(s) 222
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
RseI CAYNNNNRTG 1 cut(s) 329
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 4 cut(s) 93, 101, 186, 426
Sau3AI GATC 1 cut(s) 546
Sau96I GGNCC 1 cut(s) 273
SchI GAGTC 2 cut(s) 155, 167
ScrFI CCNGG 1 cut(s) 257
SetI ASST 6 cut(s) 46, 76, 85, 224, 281, 301
SfaNI GCATC 3 cut(s) 71, 313, 361
SinI GGWCC 1 cut(s) 273
SmiMI CAYNNNNRTG 1 cut(s) 329
SmlI CTYRAG 2 cut(s) 51, 393
SmoI CTYRAG 2 cut(s) 51, 393
Sse9I AATT 1 cut(s) 461
SsiI CCGC 4 cut(s) 93, 100, 111, 127
StyD4I CCNGG 1 cut(s) 255
TaiI ACGT 1 cut(s) 46
TaqI TCGA 2 cut(s) 156, 489
TaqII GACCGA 2 cut(s) 255, 302
TasI AATT 1 cut(s) 461
TatI WGTACW 1 cut(s) 399
TauI GCSGC 2 cut(s) 95, 103
TfiI GAWTC 1 cut(s) 491
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TseI GCWGC 2 cut(s) 185, 425
TspDTI ATGAA 3 cut(s) 271, 474, 532
Van91I CCANNNNNTGG 1 cut(s) 334
VpaK11BI GGWCC 1 cut(s) 273
XcmI CCANNNNNNNNNTGG 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.