Rmu_sc0002464.1_g000006

VIT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002464.1
OG0015687
Physical Location & Seq
Reverse (-)
27325 .. 29342
2018 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 648 bp
atgaatcgctcacccagccacgctgccaccgccatcgtaggtaaaaatgaagttacggatgtgctggtgctaggatttgcaaatctggtggcagatgggatatctatgggagttggagattttttgtccagcagcggcgaaaaggatgttgccgccatagaaatggcagtgacggaatgggatgtcacaaatcacggcggccttgagaagatggagttgctccaaaagtatcaagcacttgggatggacgttgatgatgccgccacggttgttagcattttggccaagtacaaagacatcttggtgcacgagaagatgatggctcatggaaggctaccgccagaagaagcagaaaagccatggaagaacggccttgtaacatttgctgccttccttgtctttggcactgctcctctcctgtcttttattgtcctcatcccatttacgaacagtgacttggttaagtttggtggtgcctgtatcctctctgcgcttgcactcacgcttcttgggattgcgaaggccaagattgcaggccagaaatatgctttccctgtggtagtgactctcttcactggtgctgccgccgcagctgccgcttatgccgttggatggacactgaacaatatagctggcctagaaggttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

215

Amino Acids

22.52

Weight (kDa)

5.16

Isoelectric Point (pI)

29.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 15 - 204 4.4e-43 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 473
AciI CCGC 9 cut(s) 30, 135, 153, 198, 261, 338, 585, 588, 597
AcoI YGGCCR 1 cut(s) 282
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 1 cut(s) 612
AjuI GAANNNNNNNTTGG 2 cut(s) 440, 472
AluBI AGCT 2 cut(s) 593, 632
AluI AGCT 2 cut(s) 593, 632
Alw21I GWGCWC 1 cut(s) 309
Alw44I GTGCAC 1 cut(s) 305
AoxI GGCC 6 cut(s) 199, 282, 370, 522, 535, 634
ApaLI GTGCAC 1 cut(s) 305
ApeKI GCWGC 6 cut(s) 23, 132, 386, 581, 590, 593
AspLEI GCGC 1 cut(s) 493
AsuHPI GGTGA 1 cut(s) 3
BaeGI GKGCMC 1 cut(s) 309
BalI TGGCCA 1 cut(s) 284
BanI GGYRCC 1 cut(s) 473
BauI CACGAG 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 6 cut(s) 10, 144, 373, 568, 580, 602
BccI CCATC 6 cut(s) 41, 89, 205, 238, 313, 606
BceAI ACGGC 3 cut(s) 211, 385, 590
BciVI GTATCC 1 cut(s) 491
BfaI CTAG 2 cut(s) 71, 638
BfuI GTATCC 1 cut(s) 491
BmiI GGNNCC 1 cut(s) 475
BmsI GCATC 1 cut(s) 247
BpuEI CTTGAG 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 264, 359
Bsc4I CCNNNNNNNGG 1 cut(s) 612
Bse1I ACTGG 1 cut(s) 580
BseDI CCNNGG 2 cut(s) 264, 359
BseGI GGATG 6 cut(s) 64, 151, 187, 249, 435, 617
BseLI CCNNNNNNNGG 1 cut(s) 612
BseNI ACTGG 1 cut(s) 580
BseRI GAGGAG 1 cut(s) 402
BseSI GKGCMC 1 cut(s) 309
BseXI GCAGC 6 cut(s) 10, 144, 373, 568, 580, 602
BseYI CCCAGC 1 cut(s) 14
BshFI GGCC 6 cut(s) 201, 284, 372, 524, 537, 636
BshNI GGYRCC 1 cut(s) 473
BsiHKAI GWGCWC 1 cut(s) 309
BslI CCNNNNNNNGG 1 cut(s) 612
BsnI GGCC 6 cut(s) 201, 284, 372, 524, 537, 636
Bsp1286I GDGCHC 1 cut(s) 309
Bsp19I CCATGG 1 cut(s) 359
BspACI CCGC 9 cut(s) 30, 135, 153, 198, 261, 338, 585, 588, 597
BspANI GGCC 6 cut(s) 201, 284, 372, 524, 537, 636
BspLI GGNNCC 1 cut(s) 475
BspT107I GGYRCC 1 cut(s) 473
BsrI ACTGG 1 cut(s) 580
BssECI CCNNGG 2 cut(s) 264, 359
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 359
Bst2BI CACGAG 1 cut(s) 308
Bst4CI ACNGT 2 cut(s) 268, 452
Bst6I CTCTTC 1 cut(s) 575
BstC8I GCNNGC 3 cut(s) 495, 535, 634
BstDSI CCRYGG 2 cut(s) 264, 359
BstF5I GGATG 6 cut(s) 64, 151, 187, 249, 435, 617
BstHHI GCGC 1 cut(s) 493
BstMWI GCNNNNNNNGC 8 cut(s) 15, 29, 530, 587, 590, 593, 596, 602
BstSLI GKGCMC 1 cut(s) 309
BstV1I GCAGC 6 cut(s) 10, 144, 373, 568, 580, 602
BstXI CCANNNNNNTGG 1 cut(s) 163
BsuI GTATCC 1 cut(s) 491
BsuRI GGCC 6 cut(s) 201, 284, 372, 524, 537, 636
BtgI CCRYGG 2 cut(s) 264, 359
BtsCI GGATG 6 cut(s) 64, 151, 187, 249, 435, 617
BtsI GCAGTG 2 cut(s) 174, 405
BtsIMutI CAGTG 5 cut(s) 174, 405, 457, 573, 617
Cac8I GCNNGC 3 cut(s) 495, 535, 634
CfoI GCGC 1 cut(s) 493
Csp6I GTAC 1 cut(s) 289
CspCI CAANNNNNGTGG 2 cut(s) 69, 104
CviAII CATG 2 cut(s) 326, 360
CviQI GTAC 1 cut(s) 289
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
Eco130I CCWWGG 1 cut(s) 359
Eco32I GATATC 1 cut(s) 102
EcoRV GATATC 1 cut(s) 102
EcoT14I CCWWGG 1 cut(s) 359
ErhI CCWWGG 1 cut(s) 359
FaeI CATG 2 cut(s) 329, 363
FaiI YATR 7 cut(s) 107, 158, 327, 361, 546, 603, 629
FatI CATG 2 cut(s) 325, 359
FokI GGATG 6 cut(s) 71, 158, 194, 256, 422, 624
FspBI CTAG 2 cut(s) 71, 638
GlaI GCGC 1 cut(s) 492
GsaI CCCAGC 1 cut(s) 18
HaeIII GGCC 6 cut(s) 201, 284, 372, 524, 537, 636
HhaI GCGC 1 cut(s) 493
Hin1II CATG 2 cut(s) 329, 363
Hin6I GCGC 1 cut(s) 491
HinP1I GCGC 1 cut(s) 491
HinfI GANTC 2 cut(s) 4, 565
HphI GGTGA 1 cut(s) 3
Hpy166II GTNNAC 1 cut(s) 307
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 4 cut(s) 324, 400, 514, 635
HpyCH4III ACNGT 2 cut(s) 268, 452
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 4 cut(s) 80, 307, 497, 533
HpyF10VI GCNNNNNNNGC 8 cut(s) 15, 29, 530, 587, 590, 593, 596, 602
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 2 cut(s) 329, 363
HspAI GCGC 1 cut(s) 491
LmnI GCTCC 2 cut(s) 225, 415
Lsp1109I GCAGC 6 cut(s) 10, 144, 373, 568, 580, 602
LweI GCATC 1 cut(s) 247
MaeI CTAG 2 cut(s) 71, 638
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 6 cut(s) 52, 169, 184, 376, 452, 562
MboII GAAGA 5 cut(s) 220, 325, 356, 376, 562
MhlI GDGCHC 1 cut(s) 309
MlsI TGGCCA 1 cut(s) 284
MluNI TGGCCA 1 cut(s) 284
MlyI GAGTC 1 cut(s) 559
MmeI TCCRAC 2 cut(s) 94, 589
MnlI CCTC 3 cut(s) 423, 443, 494
Mox20I TGGCCA 1 cut(s) 284
MscI TGGCCA 1 cut(s) 284
MseI TTAA 1 cut(s) 462
MslI CAYNNNNRTG 2 cut(s) 161, 302
Msp20I TGGCCA 1 cut(s) 284
MspA1I CMGCKG 2 cut(s) 135, 593
MwoI GCNNNNNNNGC 8 cut(s) 15, 29, 530, 587, 590, 593, 596, 602
NcoI CCATGG 1 cut(s) 359
NlaIII CATG 2 cut(s) 329, 363
NlaIV GGNNCC 1 cut(s) 475
NmuCI GTSAC 4 cut(s) 169, 184, 452, 562
PfeI GAWTC 1 cut(s) 4
PleI GAGTC 1 cut(s) 559
PpsI GAGTC 1 cut(s) 559
PspFI CCCAGC 1 cut(s) 14
PspN4I GGNNCC 1 cut(s) 475
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
RseI CAYNNNNRTG 2 cut(s) 161, 302
SaqAI TTAA 1 cut(s) 462
SchI GAGTC 1 cut(s) 559
SduI GDGCHC 1 cut(s) 309
SetI ASST 5 cut(s) 43, 252, 595, 634, 646
SfaNI GCATC 1 cut(s) 247
SmiMI CAYNNNNRTG 2 cut(s) 161, 302
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
SsiI CCGC 9 cut(s) 30, 135, 153, 198, 261, 338, 585, 588, 597
SspMI CTAG 2 cut(s) 71, 638
StyI CCWWGG 1 cut(s) 359
TaaI ACNGT 2 cut(s) 268, 452
TaiI ACGT 1 cut(s) 252
TatI WGTACW 1 cut(s) 288
TauI GCSGC 7 cut(s) 138, 155, 201, 263, 587, 590, 599
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 5 cut(s) 174, 412, 457, 580, 624
TseFI GTSAC 4 cut(s) 169, 184, 452, 562
TseI GCWGC 6 cut(s) 23, 132, 386, 581, 590, 593
Tsp45I GTSAC 4 cut(s) 169, 184, 452, 562
TspDTI ATGAA 2 cut(s) 17, 63
TspGWI ACGGA 2 cut(s) 71, 188
TspRI CASTG 5 cut(s) 174, 412, 457, 580, 624
VneI GTGCAC 1 cut(s) 305
XspI CTAG 2 cut(s) 71, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.