Rroxscaffold_4G00303320

VIT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015687
Physical Location & Seq
Forward (+)
23759601 .. 23761212
1612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303320.1

Sequence Viewer

Length: 816 bp
ATGGCTGCAGAAGAGGCCAACTCCAGTACTAGGCTACTTGATCCAGTCGTAGTTGACGATATTGAAGAAGATAGAGAAAGCTTTGATAACGGTGCTACTAGTTCGAGACCAAAAGAGCCATGGAAAGCAGAGTATGCCAAAAGCATTCTGTATGCTGGTCTCGATGCCATTGTCACTTGCTTCTCTCTAATCTCTTCCATCTCCGGCAGCCGTCTAACCTCCGCGGATGTGCTGGTGCTAGGATTTGCAAATCTGGTGGCGGATGGGATATCTATGGGAGTTGGAGATTTTTTGTCCAGCAGCGGCGAAAAGTATGTTGCTGCCAAAGAAATGGCGGTGACGGAATGGGATGTCACAACTCACGGCGGCCTTGAGAAGATGGAGTTGCTCCAAAAATATCAAGCACTTGGGATGGACGTTGATGATGCCGCCACGGTTGTTAGCATTTTTGGCAAGTACAACGACATCTTGGTCCACGAGAAAATGATGGCTCATGGAAGGCTACCGCCAGAAGAAGCAGAAAAGCCATGGAAGAACGGCCTTGTAACATTTGCTGCCTTCCTTGTCTTTGGCACTGCTCCTCTCCTGTCTTTTATTGTCCTCATCCCATTTACGAACAGTGACTTGGTTAAGTTTGTTGGTGCTTGTATCCTCTCTGCGCTTGCACTCACGCTTCTTGGGATTGCAAAGGCCAAGATTGCAGGCCGGAACTATGCTTTCTCCGTGGTAGTGACTCTCTTCAATGGTGCTGCTGCCGCAGCTGCCGCTTATGCCGTTGGATGGACACTGAACAATATAGCTGGCCTAGATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

271

Amino Acids

28.73

Weight (kDa)

4.67

Isoelectric Point (pI)

30.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 48 - 260 2.3e-47 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 470
AccII CGCG 1 cut(s) 224
AclWI GGATC 1 cut(s) 35
AfaI GTAC 2 cut(s) 28, 458
AfiI CCNNNNNNNGG 2 cut(s) 30, 780
AgsI TTSAA 2 cut(s) 65, 742
AhlI ACTAGT 1 cut(s) 98
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
AluBI AGCT 3 cut(s) 81, 761, 800
AluI AGCT 3 cut(s) 81, 761, 800
Alw26I GTCTC 2 cut(s) 100, 164
AlwI GGATC 1 cut(s) 35
AoxI GGCC 6 cut(s) 15, 367, 538, 690, 703, 802
ApeKI GCWGC 9 cut(s) 5, 207, 300, 320, 554, 749, 752, 758, 761
AspLEI GCGC 1 cut(s) 661
AspS9I GGNCC 1 cut(s) 472
AsuHPI GGTGA 1 cut(s) 349
AvaII GGWCC 1 cut(s) 472
BauI CACGAG 1 cut(s) 476
BbvI GCAGC 8 cut(s) 219, 307, 312, 541, 736, 739, 748, 770
BccI CCATC 7 cut(s) 206, 257, 373, 406, 481, 774, 803
BceAI ACGGC 4 cut(s) 195, 379, 553, 758
BciVI GTATCC 1 cut(s) 659
BcoDI GTCTC 2 cut(s) 100, 164
BcuI ACTAGT 1 cut(s) 98
BfaI CTAG 4 cut(s) 30, 99, 239, 806
BfmI CTRYAG 1 cut(s) 6
BfuI GTATCC 1 cut(s) 659
BmcAI AGTACT 1 cut(s) 28
Bme18I GGWCC 1 cut(s) 472
BmgT120I GGNCC 1 cut(s) 472
BmsI GCATC 2 cut(s) 154, 415
BplI GAGNNNNNCTC 1 cut(s) 37
BpmI CTGGAG 1 cut(s) 7
BpuEI CTTGAG 1 cut(s) 392
BsaI GGTCTC 2 cut(s) 100, 164
BsaJI CCNNGG 5 cut(s) 119, 222, 432, 527, 723
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 780
Bse1I ACTGG 2 cut(s) 24, 44
BseDI CCNNGG 5 cut(s) 119, 222, 432, 527, 723
BseGI GGATG 6 cut(s) 232, 268, 355, 417, 603, 785
BseLI CCNNNNNNNGG 2 cut(s) 30, 780
BseNI ACTGG 2 cut(s) 24, 44
BseRI GAGGAG 1 cut(s) 570
BseXI GCAGC 8 cut(s) 219, 307, 312, 541, 736, 739, 748, 770
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 6 cut(s) 17, 369, 540, 692, 705, 804
BsiSI CCGG 2 cut(s) 204, 706
BslI CCNNNNNNNGG 2 cut(s) 30, 780
BsmAI GTCTC 2 cut(s) 100, 164
BsmI GAATGC 1 cut(s) 144
BsnI GGCC 6 cut(s) 17, 369, 540, 692, 705, 804
Bso31I GGTCTC 2 cut(s) 100, 164
Bsp143I GATC 1 cut(s) 40
Bsp19I CCATGG 2 cut(s) 119, 527
BspANI GGCC 6 cut(s) 17, 369, 540, 692, 705, 804
BspFNI CGCG 1 cut(s) 224
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 1 cut(s) 35
BspTNI GGTCTC 2 cut(s) 100, 164
BsrI ACTGG 2 cut(s) 24, 44
BssECI CCNNGG 5 cut(s) 119, 222, 432, 527, 723
BssMI GATC 1 cut(s) 40
BssSI CACGAG 1 cut(s) 476
BssT1I CCWWGG 2 cut(s) 119, 527
Bst2BI CACGAG 1 cut(s) 476
Bst4CI ACNGT 3 cut(s) 92, 436, 620
Bst6I CTCTTC 3 cut(s) 6, 199, 743
BstAPI GCANNNNNTGC 1 cut(s) 134
BstC8I GCNNGC 3 cut(s) 663, 703, 802
BstDSI CCRYGG 5 cut(s) 119, 222, 432, 527, 723
BstF5I GGATG 6 cut(s) 232, 268, 355, 417, 603, 785
BstFNI CGCG 1 cut(s) 224
BstHHI GCGC 1 cut(s) 661
BstKTI GATC 1 cut(s) 43
BstMAI GTCTC 2 cut(s) 100, 164
BstMBI GATC 1 cut(s) 40
BstMWI GCNNNNNNNGC 9 cut(s) 14, 134, 450, 698, 755, 758, 761, 764, 770
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 8 cut(s) 219, 307, 312, 541, 736, 739, 748, 770
BstXI CCANNNNNNTGG 1 cut(s) 331
BsuI GTATCC 1 cut(s) 659
BsuRI GGCC 6 cut(s) 17, 369, 540, 692, 705, 804
BtgI CCRYGG 5 cut(s) 119, 222, 432, 527, 723
BtsCI GGATG 6 cut(s) 232, 268, 355, 417, 603, 785
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 3 cut(s) 573, 625, 785
Cac8I GCNNGC 3 cut(s) 663, 703, 802
CfoI GCGC 1 cut(s) 661
Cfr13I GGNCC 1 cut(s) 472
Cfr42I CCGCGG 1 cut(s) 225
Csp6I GTAC 2 cut(s) 27, 457
CspCI CAANNNNNGTGG 2 cut(s) 237, 272
CviAII CATG 3 cut(s) 120, 494, 528
CviQI GTAC 2 cut(s) 27, 457
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
DrdI GACNNNNNNGTC 1 cut(s) 470
DseDI GACNNNNNNGTC 1 cut(s) 470
Eam1104I CTCTTC 3 cut(s) 6, 199, 743
EarI CTCTTC 3 cut(s) 6, 199, 743
EciI GGCGGA 1 cut(s) 275
Eco130I CCWWGG 2 cut(s) 119, 527
Eco31I GGTCTC 2 cut(s) 100, 164
Eco32I GATATC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 472
EcoRV GATATC 1 cut(s) 270
EcoT14I CCWWGG 2 cut(s) 119, 527
ErhI CCWWGG 2 cut(s) 119, 527
FaeI CATG 3 cut(s) 123, 497, 531
FatI CATG 3 cut(s) 119, 493, 527
FokI GGATG 6 cut(s) 239, 275, 362, 424, 590, 792
FspBI CTAG 4 cut(s) 30, 99, 239, 806
GlaI GCGC 1 cut(s) 660
GsuI CTGGAG 1 cut(s) 7
HaeIII GGCC 6 cut(s) 17, 369, 540, 692, 705, 804
HapII CCGG 2 cut(s) 204, 706
HhaI GCGC 1 cut(s) 661
Hin1II CATG 3 cut(s) 123, 497, 531
Hin6I GCGC 1 cut(s) 659
HinP1I GCGC 1 cut(s) 659
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 1 cut(s) 733
HpaII CCGG 2 cut(s) 204, 706
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 2 cut(s) 55, 475
Hpy188III TCNNGA 2 cut(s) 105, 161
Hpy8I GTNNAC 2 cut(s) 55, 475
HpyAV CCTTC 2 cut(s) 492, 568
HpyCH4III ACNGT 3 cut(s) 92, 436, 620
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 5 cut(s) 8, 248, 665, 686, 701
HpyF10VI GCNNNNNNNGC 9 cut(s) 14, 134, 450, 698, 755, 758, 761, 764, 770
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 3 cut(s) 123, 497, 531
HspAI GCGC 1 cut(s) 659
KspI CCGCGG 1 cut(s) 225
Kzo9I GATC 1 cut(s) 40
LmnI GCTCC 2 cut(s) 393, 583
Lsp1109I GCAGC 8 cut(s) 219, 307, 312, 541, 736, 739, 748, 770
LweI GCATC 2 cut(s) 154, 415
MaeI CTAG 4 cut(s) 30, 99, 239, 806
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 6 cut(s) 172, 337, 352, 544, 620, 730
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MboII GAAGA 8 cut(s) 23, 77, 80, 186, 388, 524, 544, 730
MlyI GAGTC 1 cut(s) 727
MmeI TCCRAC 2 cut(s) 262, 757
MnlI CCTC 5 cut(s) 7, 229, 591, 611, 662
MseI TTAA 1 cut(s) 630
MspA1I CMGCKG 3 cut(s) 224, 303, 761
MspI CCGG 2 cut(s) 204, 706
Mva1269I GAATGC 1 cut(s) 144
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 9 cut(s) 14, 134, 450, 698, 755, 758, 761, 764, 770
NcoI CCATGG 2 cut(s) 119, 527
NdeII GATC 1 cut(s) 40
NlaIII CATG 3 cut(s) 123, 497, 531
NmuCI GTSAC 5 cut(s) 172, 337, 352, 620, 730
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PctI GAATGC 1 cut(s) 144
PleI GAGTC 1 cut(s) 727
PpsI GAGTC 1 cut(s) 727
PspPI GGNCC 1 cut(s) 472
PstI CTGCAG 1 cut(s) 10
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 2 cut(s) 28, 458
RsaNI GTAC 2 cut(s) 27, 457
SacII CCGCGG 1 cut(s) 225
SaqAI TTAA 1 cut(s) 630
Sau3AI GATC 1 cut(s) 40
Sau96I GGNCC 1 cut(s) 472
ScaI AGTACT 1 cut(s) 28
SchI GAGTC 1 cut(s) 727
SetI ASST 5 cut(s) 83, 221, 420, 763, 802
SfaNI GCATC 2 cut(s) 154, 415
SfcI CTRYAG 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 225
SgrBI CCGCGG 1 cut(s) 225
SinI GGWCC 1 cut(s) 472
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SpeI ACTAGT 1 cut(s) 98
SspMI CTAG 4 cut(s) 30, 99, 239, 806
StyI CCWWGG 2 cut(s) 119, 527
TaaI ACNGT 3 cut(s) 92, 436, 620
TaiI ACGT 1 cut(s) 420
TaqI TCGA 2 cut(s) 104, 162
TatI WGTACW 2 cut(s) 26, 456
TauI GCSGC 5 cut(s) 306, 369, 431, 758, 767
Tru1I TTAA 1 cut(s) 630
Tru9I TTAA 1 cut(s) 630
TscAI CASTG 3 cut(s) 580, 625, 792
TseFI GTSAC 5 cut(s) 172, 337, 352, 620, 730
TseI GCWGC 9 cut(s) 5, 207, 300, 320, 554, 749, 752, 758, 761
Tsp45I GTSAC 5 cut(s) 172, 337, 352, 620, 730
TspGWI ACGGA 2 cut(s) 356, 712
TspRI CASTG 3 cut(s) 580, 625, 792
VpaK11BI GGWCC 1 cut(s) 472
XcmI CCANNNNNNNNNTGG 1 cut(s) 117
XspI CTAG 4 cut(s) 30, 99, 239, 806
ZrmI AGTACT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.