Rorug01G0223800

VIT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0015687
Physical Location & Seq
Reverse (-)
32167584 .. 32168463
880 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0223800.1

Sequence Viewer

Length: 480 bp
ATGGGATTGTTAATGAGTTCCATGTCCCTAGGGTCCGCAAAGATGATCAATTATGCAATGGAACCGGTGTACGACAAGTTTATACAGAAGTCAGTTACAGATTTAGACGAGTTTCACGCTGCCATGCTTGGTGTTTGCAGCGCTTTAAATGCTGCTATGCCCGGTAAACACTACAATGCACCCGGACTGGAGGAAGTAAAGGTTTTTTACGAAGAATGGAAAAACGCTGACCTGACTGAAAAAAAGGAGAAATTCGTCAAATTTGTGAACGACTGTGTGAAAGTTAACCAGGCTGATACCGCTACCTTGTTGACAGGAGTACTAGCGCCTCCAGCGGCCATGGCTGCTAAAAAAGCTGGGGAGAGTCTTCCCCAGCTGAAAATGATCAAGAACGTCCCAGACGTCCTTTTCGTGCCGTCGGCAACAGTAGTAGCACTCATTACCGTAAGGTTCTCCAAAAGACTTTTCATGCGAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

159

Amino Acids

17.48

Weight (kDa)

8.82

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7874 PF25284 20 - 148 6e-06 Family of unknown function (DUF7874)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 405
AciI CCGC 3 cut(s) 36, 300, 335
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 2 cut(s) 251, 260
AcyI GRCGYC 1 cut(s) 402
AfaI GTAC 2 cut(s) 71, 321
AfeI AGCGCT 1 cut(s) 142
AgeI ACCGGT 1 cut(s) 64
AjnI CCWGG 1 cut(s) 288
AjuI GAANNNNNNNTTGG 1 cut(s) 449
AluBI AGCT 2 cut(s) 356, 376
AluI AGCT 2 cut(s) 356, 376
Aor51HI AGCGCT 1 cut(s) 142
AoxI GGCC 1 cut(s) 336
ApeKI GCWGC 4 cut(s) 119, 138, 152, 344
ApoI RAATTY 2 cut(s) 251, 260
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AsiGI ACCGGT 1 cut(s) 64
AspA2I CCTAGG 1 cut(s) 28
AspLEI GCGC 2 cut(s) 143, 328
AspS9I GGNCC 1 cut(s) 33
AsuC2I CCSGG 2 cut(s) 162, 183
AvaII GGWCC 1 cut(s) 33
AvrII CCTAGG 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 359
BbvI GCAGC 4 cut(s) 106, 139, 150, 331
BceAI ACGGC 1 cut(s) 400
BciT130I CCWGG 1 cut(s) 290
BclI TGATCA 2 cut(s) 45, 384
BcnI CCSGG 2 cut(s) 162, 183
BfaI CTAG 3 cut(s) 29, 323, 478
BfoI RGCGCY 2 cut(s) 144, 329
BisI GCNGC 5 cut(s) 120, 139, 153, 336, 345
BlnI CCTAGG 1 cut(s) 28
BlsI GCNGC 5 cut(s) 121, 140, 154, 337, 346
BmcAI AGTACT 1 cut(s) 321
Bme1390I CCNGG 3 cut(s) 162, 183, 290
Bme18I GGWCC 1 cut(s) 33
BmgT120I GGNCC 1 cut(s) 33
BmiI GGNNCC 2 cut(s) 34, 63
BmrFI CCNGG 3 cut(s) 162, 183, 290
BpiI GAAGAC 1 cut(s) 359
BpmI CTGGAG 2 cut(s) 209, 315
BpuMI CCSGG 2 cut(s) 162, 183
BsaHI GRCGYC 1 cut(s) 402
BsaJI CCNNGG 2 cut(s) 28, 339
BsaWI WCCGGW 1 cut(s) 64
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 192
Bse3DI GCAATG 1 cut(s) 63
BseBI CCWGG 1 cut(s) 290
BseDI CCNNGG 2 cut(s) 28, 339
BseMI GCAATG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 192
BseXI GCAGC 4 cut(s) 106, 139, 150, 331
BseYI CCCAGC 2 cut(s) 356, 372
BshFI GGCC 1 cut(s) 338
BshTI ACCGGT 1 cut(s) 64
BsiSI CCGG 3 cut(s) 65, 162, 183
BslFI GGGAC 2 cut(s) 10, 380
BsmFI GGGAC 2 cut(s) 10, 380
BsnI GGCC 1 cut(s) 338
Bsp143I GATC 2 cut(s) 45, 384
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 3 cut(s) 36, 300, 335
BspANI GGCC 1 cut(s) 338
BspLI GGNNCC 2 cut(s) 34, 63
BsrDI GCAATG 1 cut(s) 63
BsrFI RCCGGY 1 cut(s) 64
BsrI ACTGG 1 cut(s) 192
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 2 cut(s) 28, 339
BssMI GATC 2 cut(s) 45, 384
BssNI GRCGYC 1 cut(s) 402
BssT1I CCWWGG 2 cut(s) 28, 339
Bst2UI CCWGG 1 cut(s) 290
Bst4CI ACNGT 3 cut(s) 275, 427, 445
BstACI GRCGYC 1 cut(s) 402
BstDSI CCRYGG 1 cut(s) 339
BstH2I RGCGCY 2 cut(s) 144, 329
BstHHI GCGC 2 cut(s) 143, 328
BstKTI GATC 2 cut(s) 48, 387
BstMBI GATC 2 cut(s) 45, 384
BstMWI GCNNNNNNNGC 6 cut(s) 149, 299, 332, 341, 344, 353
BstNI CCWGG 1 cut(s) 290
BstSCI CCNGG 3 cut(s) 160, 181, 288
BstV1I GCAGC 4 cut(s) 106, 139, 150, 331
BstV2I GAAGAC 1 cut(s) 359
BsuRI GGCC 1 cut(s) 338
BtgI CCRYGG 1 cut(s) 339
CfoI GCGC 2 cut(s) 143, 328
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 1 cut(s) 33
Csp6I GTAC 2 cut(s) 70, 320
CspAI ACCGGT 1 cut(s) 64
CviAII CATG 4 cut(s) 22, 124, 340, 469
CviJI RGCY 5 cut(s) 293, 338, 344, 356, 376
CviKI_1 RGCY 5 cut(s) 293, 338, 344, 356, 376
CviQI GTAC 2 cut(s) 70, 320
DpnI GATC 2 cut(s) 47, 386
DpnII GATC 2 cut(s) 45, 384
DraI TTTAAA 1 cut(s) 147
EaeI YGGCCR 1 cut(s) 336
Eco130I CCWWGG 2 cut(s) 28, 339
Eco47I GGWCC 1 cut(s) 33
Eco47III AGCGCT 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 288
EcoT14I CCWWGG 2 cut(s) 28, 339
ErhI CCWWGG 2 cut(s) 28, 339
FaeI CATG 4 cut(s) 25, 127, 343, 472
FaiI YATR 7 cut(s) 23, 54, 83, 125, 158, 341, 470
FaqI GGGAC 2 cut(s) 10, 380
FatI CATG 4 cut(s) 21, 123, 339, 468
FbaI TGATCA 2 cut(s) 45, 384
Fnu4HI GCNGC 5 cut(s) 120, 139, 153, 336, 345
Fsp4HI GCNGC 5 cut(s) 120, 139, 153, 336, 345
FspBI CTAG 3 cut(s) 29, 323, 478
GlaI GCGC 2 cut(s) 142, 327
GluI GCNGC 5 cut(s) 120, 139, 153, 336, 345
GsaI CCCAGC 2 cut(s) 360, 376
GsuI CTGGAG 2 cut(s) 209, 315
HaeII RGCGCY 2 cut(s) 144, 329
HaeIII GGCC 1 cut(s) 338
HapII CCGG 3 cut(s) 65, 162, 183
HhaI GCGC 2 cut(s) 143, 328
Hin1I GRCGYC 1 cut(s) 402
Hin1II CATG 4 cut(s) 25, 127, 343, 472
Hin6I GCGC 2 cut(s) 141, 326
HinP1I GCGC 2 cut(s) 141, 326
HincII GTYRAC 2 cut(s) 286, 312
HindII GTYRAC 2 cut(s) 286, 312
HinfI GANTC 1 cut(s) 364
HpaI GTTAAC 1 cut(s) 286
HpaII CCGG 3 cut(s) 65, 162, 183
Hpy166II GTNNAC 5 cut(s) 70, 167, 268, 286, 312
Hpy188III TCNNGA 1 cut(s) 388
Hpy8I GTNNAC 5 cut(s) 70, 167, 268, 286, 312
Hpy99I CGWCG 1 cut(s) 421
HpyCH4III ACNGT 3 cut(s) 275, 427, 445
HpyCH4IV ACGT 2 cut(s) 393, 402
HpyCH4V TGCA 3 cut(s) 56, 138, 179
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 299, 332, 341, 344, 353
HpySE526I ACGT 2 cut(s) 393, 402
Hsp92I GRCGYC 1 cut(s) 402
Hsp92II CATG 4 cut(s) 25, 127, 343, 472
HspAI GCGC 2 cut(s) 141, 326
Ksp22I TGATCA 2 cut(s) 45, 384
KspAI GTTAAC 1 cut(s) 286
Kzo9I GATC 2 cut(s) 45, 384
Lsp1109I GCAGC 4 cut(s) 106, 139, 150, 331
MaeI CTAG 3 cut(s) 29, 323, 478
MaeII ACGT 2 cut(s) 393, 402
MaeIII GTNAC 1 cut(s) 94
MalI GATC 2 cut(s) 47, 386
MboI GATC 2 cut(s) 45, 384
MboII GAAGA 2 cut(s) 224, 359
MluCI AATT 3 cut(s) 49, 251, 260
MlyI GAGTC 1 cut(s) 373
MnlI CCTC 2 cut(s) 184, 339
MseI TTAA 3 cut(s) 11, 146, 285
MslI CAYNNNNRTG 1 cut(s) 174
MspA1I CMGCKG 2 cut(s) 335, 376
MspI CCGG 3 cut(s) 65, 162, 183
MspR9I CCNGG 3 cut(s) 162, 183, 290
MvaI CCWGG 1 cut(s) 290
MwoI GCNNNNNNNGC 6 cut(s) 149, 299, 332, 341, 344, 353
NciI CCSGG 2 cut(s) 162, 183
NcoI CCATGG 1 cut(s) 339
NdeII GATC 2 cut(s) 45, 384
NlaIII CATG 4 cut(s) 25, 127, 343, 472
NlaIV GGNNCC 2 cut(s) 34, 63
PcsI WCGNNNNNNNCGW 2 cut(s) 399, 408
PinAI ACCGGT 1 cut(s) 64
PkrI GCNGC 5 cut(s) 121, 140, 154, 337, 346
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 288
PspFI CCCAGC 2 cut(s) 356, 372
PspGI CCWGG 1 cut(s) 288
PspN4I GGNNCC 2 cut(s) 34, 63
PspPI GGNCC 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 376
RsaI GTAC 2 cut(s) 71, 321
RsaNI GTAC 2 cut(s) 70, 320
RseI CAYNNNNRTG 1 cut(s) 174
SaqAI TTAA 3 cut(s) 11, 146, 285
SatI GCNGC 5 cut(s) 120, 139, 153, 336, 345
Sau3AI GATC 2 cut(s) 45, 384
Sau96I GGNCC 1 cut(s) 33
ScaI AGTACT 1 cut(s) 321
SchI GAGTC 1 cut(s) 373
ScrFI CCNGG 3 cut(s) 162, 183, 290
SetI ASST 8 cut(s) 204, 234, 308, 358, 378, 396, 405, 452
SinI GGWCC 1 cut(s) 33
SmiMI CAYNNNNRTG 1 cut(s) 174
Sse9I AATT 3 cut(s) 49, 251, 260
SsiI CCGC 3 cut(s) 36, 300, 335
SspMI CTAG 3 cut(s) 29, 323, 478
StyD4I CCNGG 3 cut(s) 160, 181, 288
StyI CCWWGG 2 cut(s) 28, 339
TaaI ACNGT 3 cut(s) 275, 427, 445
TaiI ACGT 2 cut(s) 396, 405
TasI AATT 3 cut(s) 49, 251, 260
TatI WGTACW 1 cut(s) 319
TauI GCSGC 1 cut(s) 338
Tru1I TTAA 3 cut(s) 11, 146, 285
Tru9I TTAA 3 cut(s) 11, 146, 285
TseI GCWGC 4 cut(s) 119, 138, 152, 344
TspDTI ATGAA 1 cut(s) 457
VpaK11BI GGWCC 1 cut(s) 33
XapI RAATTY 2 cut(s) 251, 260
XmaJI CCTAGG 1 cut(s) 28
XspI CTAG 3 cut(s) 29, 323, 478
ZraI GACGTC 1 cut(s) 403
ZrmI AGTACT 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.