Rmu_sc0002547.1_g000021

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002547.1
OG0011678
Physical Location & Seq
Forward (+)
99116 .. 101509
2394 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1914 bp
atggcgttggggaataggtcgcgcagtagcagtaacaagcggtcgtcttcaagctcttacacatcaaccgtgatggtaatgggattcatagcactgtgtgtggttggtgtgtggatgatgaattactccagcgctggagtttcaacacaaaccaccacggctcgaaccaacaccgtcacttccactgcctcctcggatgaaaacaatacacaagccgctgagaataacaataataacaatgagaaacccaaggcatttgaagacaatcccggtgatcttccggacgatgccataaaaaatgatgatgggcctaacaaatcggatgctgctgctatcaacgagggaaagacgtccagagaaaaacaagagcaagatgcagctgaagaacagaaagaggatcagaagcagcaagagcttaaccaagcacagttatctgaggaaaccactatacctgaaaaccaacaagctgaacaaaccgccacacaagagaagatcatcactgctgatgatcaacctgacgagaaaaaccatcaacaaaaaaacaataacggcggccagagcggtggaaatgatcaaatagatttagaaagcaaacctcaagagcaacctgcagaaacaaattccgataatagcaagcaaaataataatgctcaagcttttcaggacaaaaaaccggggaatgcagatcaaactcagatgcaacaacaacaacaacaacaggagcagcagcaaccactaaaacaccagcaccaacaacaacaacaagaagagcagtcgctaggtgttgaaagaaatgaaaattcacaggagtcccagaataatattcaaggctcgtcaaatcaacaacaacaacagcagcagcagcaggagaatcagcatcaacatcatagtcaggaacaagagcatcatgaccaacaaaaaccgcaacaatacaccgacgagacaaaccatgtcacaacgctaccaactatacctgaccagaatgaacaaaagaaaaaccaaggagaacaacaacaacaacaacaacaacagcaacaagaagaatcttaccctcagcgaaagaaaggtaatgcagcagctgatcaaaacaataagggcaccgagtccaactcgggcgaaacgctactcaacggcggggacagctcgggcattccgaaagagtcaaaggagtcgaagaagtcgtggaggactcaagcggatcagtcggagaacgagaaggagaggcgcaaggacgaaaaggagggtcaagatagcatctacgggtacacgtggcagctttgcaatgtgactgcaggtcctgattatataccttgtttggacaatgagaaggctataaagaagctgcgttctaccaaacacttccagcatcgagagaggcattgtccgcaggagggtcccacttgcctggttccacatccggagggttacaagacgtctatccagtggccgaaaagcagagacaagatatggtatcacaatgtaccgcacacattgctggcggaggtgaagggacaccagaactgggttaaggtgtccggtgagtttttgacttttccgggtggtgggacccaattcatacatggcgctctcctctacattgatttccttcaaaacgcagtaccgaatctcgcatggggaaagcacactcgagttatactggacgttgggtgtggtgttgcgagctttgggggttatctctttgacagagatgttcttacaatgtcttttgcacccaaagatgagcatgaagctcaagttcagtttgccctggaaaggggtattccagccatttcagcagtcatgggtactcagaggctgcccttccccagcagggtctttgatgccgtccactgtgcacgttgcagagtgccttggcacctcgaaggttctgtccatcataattacgtggtttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

637

Amino Acids

71.71

Weight (kDa)

5.36

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 434 - 630 4.4e-100 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011678)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51070 AT3G51070
fragaria_vesca FvH4_1g12411
malus_domestica MD02G1136800.v1.1 MD15G1250600.v1.1
prunus_persica Prupe.7G163700_v2.0.a1
pyrus_communis pycom15g22090
rosa_chinensis RchiOBHm_Chr2g0100281
rosa_laevigata RLG00000016952
rosa_multiflora Rmu_sc0002547.1_g000021 Rmu_sc0002547.1_g000022
rosa_roxburghii Rroxscaffold_2G00142420
rosa_rugosa Rorug02G0087100 Rorug02G0087200
rosa_samantha Rh2AG136100 Rh2BG139700 Rh2CG141400 Rh2DG141600
rosa_wichuraiana Rw2G010650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 353, 1436
Acc36I ACCTGC 2 cut(s) 616, 1283
AccB1I GGYRCC 2 cut(s) 1097, 1875
AccBSI CCGCTC 1 cut(s) 561
AccII CGCG 1 cut(s) 22
AccIII TCCGGA 2 cut(s) 280, 1417
AclWI GGATC 2 cut(s) 405, 1206
AcoI YGGCCR 2 cut(s) 553, 1445
AcsI RAATTY 2 cut(s) 619, 799
AcuI CTGAAG 1 cut(s) 402
AcvI CACGTG 1 cut(s) 1269
AcyI GRCGYC 2 cut(s) 350, 1433
AdeI CACNNNGTG 1 cut(s) 98
AfaI GTAC 4 cut(s) 1265, 1482, 1620, 1807
AfeI AGCGCT 1 cut(s) 133
AfiI CCNNNNNNNGG 6 cut(s) 1391, 1522, 1562, 1773, 1774, 1831
AflIII ACRYGT 1 cut(s) 1266
AgsI TTSAA 6 cut(s) 51, 144, 260, 788, 827, 1610
AhdI GACNNNNNGTC 1 cut(s) 1293
AjnI CCWGG 2 cut(s) 1404, 1767
Alw21I GWGCWC 1 cut(s) 1858
Alw26I GTCTC 2 cut(s) 935, 1452
Alw44I GTGCAC 1 cut(s) 1854
AlwI GGATC 2 cut(s) 405, 1206
AlwNI CAGNNNCTG 3 cut(s) 1079, 1298, 1816
Ama87I CYCGRG 3 cut(s) 1111, 1144, 1647
Aor13HI TCCGGA 2 cut(s) 280, 1417
Aor51HI AGCGCT 1 cut(s) 133
AoxI GGCC 3 cut(s) 308, 553, 1445
ApaLI GTGCAC 1 cut(s) 1854
ApoI RAATTY 2 cut(s) 619, 799
ArsI GACNNNNNNTTYG 2 cut(s) 1875, 1907
AspLEI GCGC 4 cut(s) 24, 134, 1227, 1586
AspS9I GGNCC 4 cut(s) 308, 1295, 1394, 1566
AsuC2I CCSGG 3 cut(s) 270, 675, 1557
AsuHPI GGTGA 3 cut(s) 284, 1516, 1550
AvaI CYCGRG 3 cut(s) 1111, 1144, 1647
AvaII GGWCC 3 cut(s) 1295, 1394, 1566
BaeGI GKGCMC 2 cut(s) 1100, 1858
BanI GGYRCC 2 cut(s) 1097, 1875
BbrPI CACGTG 1 cut(s) 1269
BbsI GAAGAC 2 cut(s) 39, 267
Bbv12I GWGCWC 1 cut(s) 1858
BbvCI CCTCAGC 1 cut(s) 1053
BccI CCATC 4 cut(s) 67, 299, 537, 1902
BceAI ACGGC 4 cut(s) 174, 565, 1147, 1829
BciT130I CCWGG 2 cut(s) 1406, 1769
BclI TGATCA 3 cut(s) 508, 571, 1081
BcnI CCSGG 3 cut(s) 270, 675, 1557
BcoDI GTCTC 2 cut(s) 935, 1452
BfaI CTAG 1 cut(s) 779
BfmI CTRYAG 2 cut(s) 609, 1290
BfoI RGCGCY 2 cut(s) 135, 1587
BfuAI ACCTGC 2 cut(s) 616, 1283
Bme1390I CCNGG 5 cut(s) 270, 675, 1406, 1557, 1769
Bme18I GGWCC 3 cut(s) 1295, 1394, 1566
BmeRI GACNNNNNGTC 1 cut(s) 1293
BmeT110I CYCGRG 3 cut(s) 1111, 1144, 1647
BmgT120I GGNCC 4 cut(s) 308, 1295, 1394, 1566
BmiI GGNNCC 7 cut(s) 1099, 1395, 1396, 1410, 1567, 1568, 1877
BmrFI CCNGG 5 cut(s) 270, 675, 1406, 1557, 1769
BmrI ACTGGG 1 cut(s) 1531
BmsI GCATC 9 cut(s) 277, 313, 364, 687, 886, 913, 1263, 1375, 1831
BmuI ACTGGG 1 cut(s) 1531
BpiI GAAGAC 2 cut(s) 39, 267
BplI GAGNNNNNCTC 2 cut(s) 1094, 1126
BpmI CTGGAG 2 cut(s) 112, 156
Bpu10I CCTNAGC 1 cut(s) 1053
BpuEI CTTGAG 4 cut(s) 582, 636, 1176, 1737
BpuMI CCSGG 3 cut(s) 270, 675, 1557
BsaAI YACGTR 2 cut(s) 1269, 1906
BsaHI GRCGYC 2 cut(s) 350, 1433
BsaJI CCNNGG 7 cut(s) 156, 192, 249, 674, 1000, 1767, 1871
BsaWI WCCGGW 3 cut(s) 280, 1417, 1535
Bsc4I CCNNNNNNNGG 6 cut(s) 1391, 1522, 1562, 1773, 1774, 1831
Bse1I ACTGG 3 cut(s) 1441, 1526, 1662
Bse3DI GCAATG 2 cut(s) 1288, 1490
BseAI TCCGGA 2 cut(s) 280, 1417
BseBI CCWGG 2 cut(s) 1406, 1769
BseDI CCNNGG 7 cut(s) 156, 192, 249, 674, 1000, 1767, 1871
BseGI GGATG 4 cut(s) 120, 202, 328, 1414
BseLI CCNNNNNNNGG 6 cut(s) 1391, 1522, 1562, 1773, 1774, 1831
BseMI GCAATG 2 cut(s) 1288, 1490
BseMII CTCAG 5 cut(s) 210, 426, 707, 1067, 1823
BseNI ACTGG 3 cut(s) 1441, 1526, 1662
BseRI GAGGAG 2 cut(s) 181, 1580
BseSI GKGCMC 2 cut(s) 1100, 1858
BseYI CCCAGC 1 cut(s) 1826
Bsh1236I CGCG 1 cut(s) 22
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 3 cut(s) 310, 555, 1447
BshNI GGYRCC 2 cut(s) 1097, 1875
BsiEI CGRYCG 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 1858
BsiHKCI CYCGRG 3 cut(s) 1111, 1144, 1647
BsiSI CCGG 6 cut(s) 270, 281, 674, 1418, 1536, 1556
BslFI GGGAC 5 cut(s) 796, 1151, 1380, 1524, 1579
BslI CCNNNNNNNGG 6 cut(s) 1391, 1522, 1562, 1773, 1774, 1831
BsmAI GTCTC 2 cut(s) 935, 1452
BsmFI GGGAC 5 cut(s) 796, 1151, 1380, 1524, 1579
BsmI GAATGC 2 cut(s) 685, 1149
BsnI GGCC 3 cut(s) 310, 555, 1447
BsoBI CYCGRG 3 cut(s) 1111, 1144, 1647
Bsp1286I GDGCHC 2 cut(s) 1100, 1858
Bsp13I TCCGGA 2 cut(s) 280, 1417
Bsp143I GATC 8 cut(s) 274, 397, 492, 508, 571, 685, 1081, 1198
BspANI GGCC 3 cut(s) 310, 555, 1447
BspCNI CTCAG 5 cut(s) 211, 427, 706, 1066, 1822
BspEI TCCGGA 2 cut(s) 280, 1417
BspFNI CGCG 1 cut(s) 22
BspHI TCATGA 1 cut(s) 907
BspLI GGNNCC 7 cut(s) 1099, 1395, 1396, 1410, 1567, 1568, 1877
BspMAI CTGCAG 2 cut(s) 613, 1294
BspMI ACCTGC 2 cut(s) 616, 1283
BspPI GGATC 2 cut(s) 405, 1206
BspQI GCTCTTC 1 cut(s) 762
BspT107I GGYRCC 2 cut(s) 1097, 1875
BsrBI CCGCTC 1 cut(s) 561
BsrDI GCAATG 2 cut(s) 1288, 1490
BsrI ACTGG 3 cut(s) 1441, 1526, 1662
BssECI CCNNGG 7 cut(s) 156, 192, 249, 674, 1000, 1767, 1871
BssMI GATC 8 cut(s) 274, 397, 492, 508, 571, 685, 1081, 1198
BssNI GRCGYC 2 cut(s) 350, 1433
BssT1I CCWWGG 3 cut(s) 249, 1000, 1871
Bst2UI CCWGG 2 cut(s) 1406, 1769
Bst4CI ACNGT 5 cut(s) 70, 96, 175, 429, 1853
Bst6I CTCTTC 1 cut(s) 762
BstACI GRCGYC 2 cut(s) 350, 1433
BstAPI GCANNNNNTGC 1 cut(s) 1492
BstBAI YACGTR 2 cut(s) 1269, 1906
BstC8I GCNNGC 3 cut(s) 635, 1497, 1681
BstDEI CTNAG 5 cut(s) 219, 435, 693, 1053, 1809
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 4 cut(s) 120, 202, 328, 1414
BstFNI CGCG 1 cut(s) 22
BstH2I RGCGCY 2 cut(s) 135, 1587
BstHHI GCGC 4 cut(s) 24, 134, 1227, 1586
BstKTI GATC 8 cut(s) 277, 400, 495, 511, 574, 688, 1084, 1201
BstMAI GTCTC 2 cut(s) 935, 1452
BstMBI GATC 8 cut(s) 274, 397, 492, 508, 571, 685, 1081, 1198
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 7 cut(s) 412, 558, 862, 1140, 1384, 1492, 1793
BstNI CCWGG 2 cut(s) 1406, 1769
BstSCI CCNGG 5 cut(s) 268, 673, 1404, 1555, 1767
BstSFI CTRYAG 2 cut(s) 609, 1290
BstSLI GKGCMC 2 cut(s) 1100, 1858
BstUI CGCG 1 cut(s) 22
BstV2I GAAGAC 2 cut(s) 39, 267
BstXI CCANNNNNNTGG 2 cut(s) 563, 1405
BsuRI GGCC 3 cut(s) 310, 555, 1447
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 4 cut(s) 120, 202, 328, 1414
BtsI GCAGTG 2 cut(s) 183, 498
BtsIMutI CAGTG 5 cut(s) 92, 183, 498, 1448, 1849
BveI ACCTGC 2 cut(s) 616, 1283
Cac8I GCNNGC 3 cut(s) 635, 1497, 1681
CaiI CAGNNNCTG 3 cut(s) 1079, 1298, 1816
CciI TCATGA 1 cut(s) 907
CfoI GCGC 4 cut(s) 24, 134, 1227, 1586
Cfr13I GGNCC 4 cut(s) 308, 1295, 1394, 1566
Csp6I GTAC 4 cut(s) 1264, 1481, 1619, 1806
CviAII CATG 6 cut(s) 908, 950, 1580, 1632, 1745, 1801
CviQI GTAC 4 cut(s) 1264, 1481, 1619, 1806
DdeI CTNAG 5 cut(s) 219, 435, 693, 1053, 1809
DpnI GATC 8 cut(s) 276, 399, 494, 510, 573, 687, 1083, 1200
DpnII GATC 8 cut(s) 274, 397, 492, 508, 571, 685, 1081, 1198
DraIII CACNNNGTG 1 cut(s) 98
DriI GACNNNNNGTC 1 cut(s) 1293
EaeI YGGCCR 2 cut(s) 553, 1445
Eam1104I CTCTTC 1 cut(s) 762
Eam1105I GACNNNNNGTC 1 cut(s) 1293
EarI CTCTTC 1 cut(s) 762
EciI GGCGGA 1 cut(s) 1514
Eco130I CCWWGG 3 cut(s) 249, 1000, 1871
Eco47I GGWCC 3 cut(s) 1295, 1394, 1566
Eco47III AGCGCT 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 402
Eco72I CACGTG 1 cut(s) 1269
Eco88I CYCGRG 3 cut(s) 1111, 1144, 1647
EcoO109I RGGNCCY 3 cut(s) 1295, 1394, 1566
EcoRII CCWGG 2 cut(s) 1404, 1767
EcoT14I CCWWGG 3 cut(s) 249, 1000, 1871
ErhI CCWWGG 3 cut(s) 249, 1000, 1871
FaeI CATG 6 cut(s) 911, 953, 1583, 1635, 1748, 1804
FaqI GGGAC 5 cut(s) 796, 1151, 1380, 1524, 1579
FatI CATG 6 cut(s) 907, 949, 1579, 1631, 1744, 1800
FauI CCCGC 1 cut(s) 1127
FbaI TGATCA 3 cut(s) 508, 571, 1081
FokI GGATG 4 cut(s) 127, 209, 335, 1401
FspBI CTAG 1 cut(s) 779
GlaI GCGC 4 cut(s) 23, 133, 1226, 1585
GsaI CCCAGC 1 cut(s) 1830
GsuI CTGGAG 2 cut(s) 112, 156
HaeII RGCGCY 2 cut(s) 135, 1587
HaeIII GGCC 3 cut(s) 310, 555, 1447
HapII CCGG 6 cut(s) 270, 281, 674, 1418, 1536, 1556
HhaI GCGC 4 cut(s) 24, 134, 1227, 1586
Hin1I GRCGYC 2 cut(s) 350, 1433
Hin1II CATG 6 cut(s) 911, 953, 1583, 1635, 1748, 1804
Hin6I GCGC 4 cut(s) 22, 132, 1225, 1584
HinP1I GCGC 4 cut(s) 22, 132, 1225, 1584
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 9 cut(s) 84, 809, 871, 1043, 1103, 1160, 1169, 1189, 1624
HpaII CCGG 6 cut(s) 270, 281, 674, 1418, 1536, 1556
HphI GGTGA 3 cut(s) 284, 1516, 1550
Hpy166II GTNNAC 3 cut(s) 1266, 1849, 1856
Hpy188I TCNGA 9 cut(s) 196, 322, 402, 436, 625, 696, 1155, 1207, 1812
Hpy8I GTNNAC 3 cut(s) 1266, 1849, 1856
Hpy99I CGWCG 1 cut(s) 941
HpyAV CCTTC 6 cut(s) 1210, 1321, 1501, 1616, 1831, 1877
HpyCH4III ACNGT 5 cut(s) 70, 96, 175, 429, 1853
HpyCH4IV ACGT 6 cut(s) 350, 1268, 1433, 1662, 1858, 1905
HpyF10VI GCNNNNNNNGC 7 cut(s) 412, 558, 862, 1140, 1384, 1492, 1793
HpyF3I CTNAG 5 cut(s) 219, 435, 693, 1053, 1809
HpySE526I ACGT 6 cut(s) 350, 1268, 1433, 1662, 1858, 1905
Hsp92I GRCGYC 2 cut(s) 350, 1433
Hsp92II CATG 6 cut(s) 911, 953, 1583, 1635, 1748, 1804
HspAI GCGC 4 cut(s) 22, 132, 1225, 1584
KflI GGGWCCC 2 cut(s) 1394, 1566
Kpn2I TCCGGA 2 cut(s) 280, 1417
Ksp22I TGATCA 3 cut(s) 508, 571, 1081
Kzo9I GATC 8 cut(s) 274, 397, 492, 508, 571, 685, 1081, 1198
LguI GCTCTTC 1 cut(s) 762
LmnI GCTCC 1 cut(s) 721
LweI GCATC 9 cut(s) 277, 313, 364, 687, 886, 913, 1263, 1375, 1831
MaeI CTAG 1 cut(s) 779
MaeII ACGT 6 cut(s) 350, 1268, 1433, 1662, 1858, 1905
MaeIII GTNAC 5 cut(s) 32, 175, 952, 1285, 1424
MalI GATC 8 cut(s) 276, 399, 494, 510, 573, 687, 1083, 1200
MbiI CCGCTC 1 cut(s) 561
MboI GATC 8 cut(s) 274, 397, 492, 508, 571, 685, 1081, 1198
MboII GAAGA 8 cut(s) 39, 269, 272, 395, 502, 779, 1052, 1186
MhlI GDGCHC 2 cut(s) 1100, 1858
MluCI AATT 5 cut(s) 121, 619, 799, 1571, 1900
MlyI GAGTC 5 cut(s) 818, 1112, 1169, 1178, 1183
MmeI TCCRAC 2 cut(s) 1131, 1185
MroI TCCGGA 2 cut(s) 280, 1417
MseI TTAA 2 cut(s) 417, 1527
MspA1I CMGCKG 3 cut(s) 218, 380, 1079
MspI CCGG 6 cut(s) 270, 281, 674, 1418, 1536, 1556
MspR9I CCNGG 5 cut(s) 270, 675, 1406, 1557, 1769
Mva1269I GAATGC 2 cut(s) 685, 1149
MvaI CCWGG 2 cut(s) 1406, 1769
MvnI CGCG 1 cut(s) 22
MwoI GCNNNNNNNGC 7 cut(s) 412, 558, 862, 1140, 1384, 1492, 1793
NciI CCSGG 3 cut(s) 270, 675, 1557
NdeII GATC 8 cut(s) 274, 397, 492, 508, 571, 685, 1081, 1198
NlaIII CATG 6 cut(s) 911, 953, 1583, 1635, 1748, 1804
NlaIV GGNNCC 7 cut(s) 1099, 1395, 1396, 1410, 1567, 1568, 1877
NmuCI GTSAC 3 cut(s) 175, 952, 1285
PaeR7I CTCGAG 1 cut(s) 1647
PagI TCATGA 1 cut(s) 907
PciSI GCTCTTC 1 cut(s) 762
PcsI WCGNNNNNNNCGW 2 cut(s) 1151, 1178
PctI GAATGC 2 cut(s) 685, 1149
PfeI GAWTC 4 cut(s) 84, 871, 1043, 1624
PleI GAGTC 5 cut(s) 817, 1111, 1168, 1177, 1183
PmaCI CACGTG 1 cut(s) 1269
PmlI CACGTG 1 cut(s) 1269
PpsI GAGTC 5 cut(s) 817, 1111, 1168, 1177, 1183
Ppu21I YACGTR 2 cut(s) 1269, 1906
PpuMI RGGWCCY 3 cut(s) 1295, 1394, 1566
Psp5II RGGWCCY 3 cut(s) 1295, 1394, 1566
Psp6I CCWGG 2 cut(s) 1404, 1767
PspCI CACGTG 1 cut(s) 1269
PspFI CCCAGC 1 cut(s) 1826
PspGI CCWGG 2 cut(s) 1404, 1767
PspN4I GGNNCC 7 cut(s) 1099, 1395, 1396, 1410, 1567, 1568, 1877
PspPI GGNCC 4 cut(s) 308, 1295, 1394, 1566
PspPPI RGGWCCY 3 cut(s) 1295, 1394, 1566
PspXI VCTCGAGB 1 cut(s) 1647
PstI CTGCAG 2 cut(s) 613, 1294
PstNI CAGNNNCTG 3 cut(s) 1079, 1298, 1816
PvuII CAGCTG 2 cut(s) 380, 1079
RsaI GTAC 4 cut(s) 1265, 1482, 1620, 1807
RsaNI GTAC 4 cut(s) 1264, 1481, 1619, 1806
SapI GCTCTTC 1 cut(s) 762
SaqAI TTAA 2 cut(s) 417, 1527
Sau3AI GATC 8 cut(s) 274, 397, 492, 508, 571, 685, 1081, 1198
Sau96I GGNCC 4 cut(s) 308, 1295, 1394, 1566
SchI GAGTC 5 cut(s) 818, 1112, 1169, 1178, 1183
ScrFI CCNGG 5 cut(s) 270, 675, 1406, 1557, 1769
SduI GDGCHC 2 cut(s) 1100, 1858
SfaNI GCATC 9 cut(s) 277, 313, 364, 687, 886, 913, 1263, 1375, 1831
SfcI CTRYAG 2 cut(s) 609, 1290
Sfr274I CTCGAG 1 cut(s) 1647
SinI GGWCC 3 cut(s) 1295, 1394, 1566
SlaI CTCGAG 1 cut(s) 1647
SmlI CTYRAG 5 cut(s) 597, 651, 1191, 1647, 1752
SmoI CTYRAG 5 cut(s) 597, 651, 1191, 1647, 1752
Sse9I AATT 5 cut(s) 121, 619, 799, 1571, 1900
SspI AATATT 1 cut(s) 823
SspMI CTAG 1 cut(s) 779
StyD4I CCNGG 5 cut(s) 268, 673, 1404, 1555, 1767
StyI CCWWGG 3 cut(s) 249, 1000, 1871
TaaI ACNGT 5 cut(s) 70, 96, 175, 429, 1853
TaiI ACGT 6 cut(s) 353, 1271, 1436, 1665, 1861, 1908
TaqI TCGA 5 cut(s) 163, 1172, 1369, 1648, 1881
TasI AATT 5 cut(s) 121, 619, 799, 1571, 1900
TauI GCSGC 2 cut(s) 218, 555
TfiI GAWTC 4 cut(s) 84, 871, 1043, 1624
Tru1I TTAA 2 cut(s) 417, 1527
Tru9I TTAA 2 cut(s) 417, 1527
TscAI CASTG 5 cut(s) 99, 190, 505, 1448, 1856
TseFI GTSAC 3 cut(s) 175, 952, 1285
Tsp45I GTSAC 3 cut(s) 175, 952, 1285
TspDTI ATGAA 7 cut(s) 76, 134, 213, 810, 999, 1564, 1761
TspRI CASTG 5 cut(s) 99, 190, 505, 1448, 1856
VneI GTGCAC 1 cut(s) 1854
VpaK11BI GGWCC 3 cut(s) 1295, 1394, 1566
XapI RAATTY 2 cut(s) 619, 799
XcmI CCANNNNNNNNNTGG 1 cut(s) 1577
XhoI CTCGAG 1 cut(s) 1647
XspI CTAG 1 cut(s) 779
ZraI GACGTC 2 cut(s) 351, 1434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.