Rmu_sc0002547.1_g000022

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002547.1
OG0011678
Physical Location & Seq
Forward (+)
101798 .. 103266
1469 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 858 bp
atgactgccttaacaaagtctatgtgttgggaccttattacgatcaagaacaacaaagtaaatttagttgccgctgccatctaccgcaagccaacctcgaaccaatgctacgaacaaagaaagcaaaagcaacctcctatgtgcaaaaccgatgatgatcccaatgctgcctggtatgtacctctgcaggcatgtatgcacagggttcctgttgacaaatctgagagagggactcaatggcctgagaactggcctaatcgactggaaactcctccttactggttaaacagctcccggatgggtatttatggaaaaccagcaccccaagattttgcaagagattatgaacactggaaacgtgtgatcaacaacagttacatgaaaagtttgggtatcaattggtccggagtcagaaatgtcatggatatgagagcggtttatggagggtttgccgccgctctcaaggatcttaaggtctgggtgatgaatgtggtgaatgtagattctgcagatactctccccatcatctatgaacgtggtctttttggaatttatcatgattggtgtgaatcttttagcacctacccaagaacctatgacctgctccatgcagatcatctcttttcttcgctcaaaaagaggtgcagacttcctccggtaatggcagaaattgatcggattgtcaggccgggaggcaaattgatcgtccgcgatgaatccagcgcaattggggaggttgagaacttgttgaagtccctgcattgggaggttcacttaaccttctccaaaaaccaagaagggatgcttagtgcacagaaaggcaattggcggccaaccacatcggcagatacttcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

285

Amino Acids

32.8

Weight (kDa)

9.02

Isoelectric Point (pI)

29.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 1 - 272 5.8e-106 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011678)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51070 AT3G51070
fragaria_vesca FvH4_1g12411
malus_domestica MD02G1136800.v1.1 MD15G1250600.v1.1
prunus_persica Prupe.7G163700_v2.0.a1
pyrus_communis pycom15g22090
rosa_chinensis RchiOBHm_Chr2g0100281
rosa_laevigata RLG00000016952
rosa_multiflora Rmu_sc0002547.1_g000021 Rmu_sc0002547.1_g000022
rosa_roxburghii Rroxscaffold_2G00142420
rosa_rugosa Rorug02G0087100 Rorug02G0087200
rosa_samantha Rh2AG136100 Rh2BG139700 Rh2CG141400 Rh2DG141600
rosa_wichuraiana Rw2G010650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 609
AccBSI CCGCTC 2 cut(s) 434, 458
AccII CGCG 1 cut(s) 711
AccIII TCCGGA 1 cut(s) 404
AciI CCGC 7 cut(s) 72, 85, 434, 453, 456, 709, 829
AclWI GGATC 2 cut(s) 152, 474
AcoI YGGCCR 1 cut(s) 830
AcsI RAATTY 2 cut(s) 61, 549
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 2 cut(s) 762, 763
AflII CTTAAG 1 cut(s) 470
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 1 cut(s) 751
AjnI CCWGG 1 cut(s) 170
AluBI AGCT 1 cut(s) 291
AluI AGCT 1 cut(s) 291
Alw21I GWGCWC 1 cut(s) 814
Alw44I GTGCAC 1 cut(s) 810
AlwI GGATC 2 cut(s) 152, 474
Aor13HI TCCGGA 1 cut(s) 404
AoxI GGCC 4 cut(s) 239, 251, 686, 830
ApaLI GTGCAC 1 cut(s) 810
ApeKI GCWGC 2 cut(s) 74, 167
ApoI RAATTY 2 cut(s) 61, 549
ArsI GACNNNNNNTTYG 2 cut(s) 690, 722
AspLEI GCGC 1 cut(s) 725
AspS9I GGNCC 2 cut(s) 31, 402
AsuC2I CCSGG 2 cut(s) 295, 690
AsuHPI GGTGA 2 cut(s) 493, 505
AvaII GGWCC 2 cut(s) 31, 402
BaeGI GKGCMC 1 cut(s) 814
Bbv12I GWGCWC 1 cut(s) 814
BbvI GCAGC 2 cut(s) 61, 154
BccI CCATC 3 cut(s) 86, 292, 530
BcgI CGANNNNNNTGC 2 cut(s) 685, 719
BciT130I CCWGG 1 cut(s) 172
BclI TGATCA 1 cut(s) 363
BcnI CCSGG 2 cut(s) 295, 690
BfmI CTRYAG 2 cut(s) 185, 507
BfrI CTTAAG 1 cut(s) 470
BfuAI ACCTGC 1 cut(s) 609
BisI GCNGC 6 cut(s) 72, 75, 168, 453, 456, 830
BlsI GCNGC 6 cut(s) 73, 76, 169, 454, 457, 831
Bme1390I CCNGG 3 cut(s) 172, 295, 690
Bme18I GGWCC 2 cut(s) 31, 402
BmgT120I GGNCC 2 cut(s) 31, 402
BmiI GGNNCC 2 cut(s) 32, 207
BmrFI CCNGG 3 cut(s) 172, 295, 690
BmsI GCATC 1 cut(s) 792
BplI GAGNNNNNCTC 2 cut(s) 217, 249
BpuEI CTTGAG 1 cut(s) 446
BpuMI CCSGG 2 cut(s) 295, 690
BsaBI GATNNNNATC 1 cut(s) 156
BsaWI WCCGGW 2 cut(s) 404, 655
Bsc4I CCNNNNNNNGG 2 cut(s) 762, 763
Bse1I ACTGG 4 cut(s) 254, 267, 284, 356
Bse8I GATNNNNATC 1 cut(s) 156
BseAI TCCGGA 1 cut(s) 404
BseBI CCWGG 1 cut(s) 172
BseGI GGATG 2 cut(s) 303, 807
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 2 cut(s) 762, 763
BseMII CTCAG 2 cut(s) 213, 234
BseNI ACTGG 4 cut(s) 254, 267, 284, 356
BseRI GAGGAG 1 cut(s) 261
BseSI GKGCMC 1 cut(s) 814
BseXI GCAGC 2 cut(s) 61, 154
BsgI GTGCAG 1 cut(s) 664
Bsh1236I CGCG 1 cut(s) 711
BshFI GGCC 4 cut(s) 241, 253, 688, 832
BsiHKAI GWGCWC 1 cut(s) 814
BsiSI CCGG 4 cut(s) 295, 405, 656, 689
BslFI GGGAC 3 cut(s) 44, 244, 739
BslI CCNNNNNNNGG 2 cut(s) 762, 763
BsmFI GGGAC 3 cut(s) 44, 244, 739
BsnI GGCC 4 cut(s) 241, 253, 688, 832
Bsp1286I GDGCHC 1 cut(s) 814
Bsp13I TCCGGA 1 cut(s) 404
Bsp143I GATC 7 cut(s) 42, 157, 363, 466, 613, 673, 702
BspACI CCGC 7 cut(s) 72, 85, 434, 453, 456, 709, 829
BspANI GGCC 4 cut(s) 241, 253, 688, 832
BspCNI CTCAG 2 cut(s) 214, 235
BspEI TCCGGA 1 cut(s) 404
BspFNI CGCG 1 cut(s) 711
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 2 cut(s) 32, 207
BspMAI CTGCAG 2 cut(s) 189, 511
BspMI ACCTGC 1 cut(s) 609
BspPI GGATC 2 cut(s) 152, 474
BspTI CTTAAG 1 cut(s) 470
BsrBI CCGCTC 2 cut(s) 434, 458
BsrI ACTGG 4 cut(s) 254, 267, 284, 356
BssMI GATC 7 cut(s) 42, 157, 363, 466, 613, 673, 702
Bst2UI CCWGG 1 cut(s) 172
Bst4CI ACNGT 1 cut(s) 374
BstAFI CTTAAG 1 cut(s) 470
BstC8I GCNNGC 2 cut(s) 89, 189
BstDEI CTNAG 3 cut(s) 222, 243, 806
BstF5I GGATG 2 cut(s) 303, 807
BstFNI CGCG 1 cut(s) 711
BstHHI GCGC 1 cut(s) 725
BstKTI GATC 7 cut(s) 45, 160, 366, 469, 616, 676, 705
BstMBI GATC 7 cut(s) 42, 157, 363, 466, 613, 673, 702
BstNI CCWGG 1 cut(s) 172
BstNSI RCATGY 1 cut(s) 195
BstSCI CCNGG 3 cut(s) 170, 293, 688
BstSFI CTRYAG 2 cut(s) 185, 507
BstSLI GKGCMC 1 cut(s) 814
BstUI CGCG 1 cut(s) 711
BstV1I GCAGC 2 cut(s) 61, 154
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 4 cut(s) 241, 253, 688, 832
BtgZI GCGATG 1 cut(s) 726
BtsCI GGATG 2 cut(s) 303, 807
BtsIMutI CAGTG 1 cut(s) 349
BveI ACCTGC 1 cut(s) 609
Cac8I GCNNGC 2 cut(s) 89, 189
CciI TCATGA 1 cut(s) 556
CfoI GCGC 1 cut(s) 725
Cfr13I GGNCC 2 cut(s) 31, 402
Csp6I GTAC 1 cut(s) 179
CviAII CATG 5 cut(s) 192, 379, 421, 557, 608
CviJI RGCY 6 cut(s) 91, 241, 253, 291, 688, 832
CviKI_1 RGCY 6 cut(s) 91, 241, 253, 291, 688, 832
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 3 cut(s) 222, 243, 806
DpnI GATC 7 cut(s) 44, 159, 365, 468, 615, 675, 704
DpnII GATC 7 cut(s) 42, 157, 363, 466, 613, 673, 702
EaeI YGGCCR 1 cut(s) 830
Eco47I GGWCC 2 cut(s) 31, 402
EcoO109I RGGNCCY 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 170
FaeI CATG 5 cut(s) 195, 382, 424, 560, 611
FalI AAGNNNNNCTT 2 cut(s) 789, 821
FaqI GGGAC 3 cut(s) 44, 244, 739
FatI CATG 5 cut(s) 191, 378, 420, 556, 607
FbaI TGATCA 1 cut(s) 363
Fnu4HI GCNGC 6 cut(s) 72, 75, 168, 453, 456, 830
FokI GGATG 2 cut(s) 310, 814
Fsp4HI GCNGC 6 cut(s) 72, 75, 168, 453, 456, 830
GlaI GCGC 1 cut(s) 724
GluI GCNGC 6 cut(s) 72, 75, 168, 453, 456, 830
HaeIII GGCC 4 cut(s) 241, 253, 688, 832
HapII CCGG 4 cut(s) 295, 405, 656, 689
HhaI GCGC 1 cut(s) 725
Hin1II CATG 5 cut(s) 195, 382, 424, 560, 611
Hin6I GCGC 1 cut(s) 723
HinP1I GCGC 1 cut(s) 723
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 5 cut(s) 232, 408, 503, 569, 716
HpaII CCGG 4 cut(s) 295, 405, 656, 689
HphI GGTGA 2 cut(s) 493, 505
Hpy166II GTNNAC 3 cut(s) 214, 772, 812
Hpy188I TCNGA 3 cut(s) 223, 413, 678
Hpy188III TCNNGA 3 cut(s) 46, 405, 557
Hpy8I GTNNAC 3 cut(s) 214, 772, 812
HpyAV CCTTC 2 cut(s) 790, 791
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 2 cut(s) 358, 535
HpyCH4V TGCA 9 cut(s) 144, 187, 199, 335, 509, 611, 645, 760, 812
HpyF3I CTNAG 3 cut(s) 222, 243, 806
HpySE526I ACGT 2 cut(s) 358, 535
Hsp92II CATG 5 cut(s) 195, 382, 424, 560, 611
HspAI GCGC 1 cut(s) 723
Kpn2I TCCGGA 1 cut(s) 404
Ksp22I TGATCA 1 cut(s) 363
Kzo9I GATC 7 cut(s) 42, 157, 363, 466, 613, 673, 702
LmnI GCTCC 2 cut(s) 296, 609
Lsp1109I GCAGC 2 cut(s) 61, 154
LweI GCATC 1 cut(s) 792
MaeII ACGT 2 cut(s) 358, 535
MaeIII GTNAC 1 cut(s) 374
MalI GATC 7 cut(s) 44, 159, 365, 468, 615, 675, 704
MbiI CCGCTC 2 cut(s) 434, 458
MboI GATC 7 cut(s) 42, 157, 363, 466, 613, 673, 702
MboII GAAGA 1 cut(s) 618
MfeI CAATTG 3 cut(s) 397, 726, 823
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 1 cut(s) 814
MluCI AATT 7 cut(s) 61, 397, 549, 669, 698, 726, 823
MlyI GAGTC 2 cut(s) 226, 417
MroI TCCGGA 1 cut(s) 404
MseI TTAA 5 cut(s) 11, 284, 471, 776, 856
MslI CAYNNNNRTG 1 cut(s) 425
MspA1I CMGCKG 1 cut(s) 74
MspCI CTTAAG 1 cut(s) 470
MspI CCGG 4 cut(s) 295, 405, 656, 689
MspR9I CCNGG 3 cut(s) 172, 295, 690
MunI CAATTG 3 cut(s) 397, 726, 823
MvaI CCWGG 1 cut(s) 172
MvnI CGCG 1 cut(s) 711
NciI CCSGG 2 cut(s) 295, 690
NdeII GATC 7 cut(s) 42, 157, 363, 466, 613, 673, 702
NlaIII CATG 5 cut(s) 195, 382, 424, 560, 611
NlaIV GGNNCC 2 cut(s) 32, 207
NspI RCATGY 1 cut(s) 195
PagI TCATGA 1 cut(s) 556
PfeI GAWTC 3 cut(s) 503, 569, 716
PfoI TCCNGGA 1 cut(s) 293
PkrI GCNGC 6 cut(s) 73, 76, 169, 454, 457, 831
PleI GAGTC 2 cut(s) 226, 416
PpsI GAGTC 2 cut(s) 226, 416
PpuMI RGGWCCY 1 cut(s) 31
Psp5II RGGWCCY 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 170
PspGI CCWGG 1 cut(s) 170
PspN4I GGNNCC 2 cut(s) 32, 207
PspPI GGNCC 2 cut(s) 31, 402
PspPPI RGGWCCY 1 cut(s) 31
PsrI GAACNNNNNNTAC 2 cut(s) 92, 124
PstI CTGCAG 2 cut(s) 189, 511
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
RseI CAYNNNNRTG 1 cut(s) 425
SaqAI TTAA 5 cut(s) 11, 284, 471, 776, 856
SatI GCNGC 6 cut(s) 72, 75, 168, 453, 456, 830
Sau3AI GATC 7 cut(s) 42, 157, 363, 466, 613, 673, 702
Sau96I GGNCC 2 cut(s) 31, 402
SchI GAGTC 2 cut(s) 226, 417
ScrFI CCNGG 3 cut(s) 172, 295, 690
SduI GDGCHC 1 cut(s) 814
SfaNI GCATC 1 cut(s) 792
SfcI CTRYAG 2 cut(s) 185, 507
SinI GGWCC 2 cut(s) 31, 402
SmiMI CAYNNNNRTG 1 cut(s) 425
SmlI CTYRAG 2 cut(s) 461, 470
SmoI CTYRAG 2 cut(s) 461, 470
Sse9I AATT 7 cut(s) 61, 397, 549, 669, 698, 726, 823
SsiI CCGC 7 cut(s) 72, 85, 434, 453, 456, 709, 829
StyD4I CCNGG 3 cut(s) 170, 293, 688
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 2 cut(s) 361, 538
TaqI TCGA 2 cut(s) 98, 259
TasI AATT 7 cut(s) 61, 397, 549, 669, 698, 726, 823
TauI GCSGC 4 cut(s) 74, 455, 458, 832
TfiI GAWTC 3 cut(s) 503, 569, 716
Tru1I TTAA 5 cut(s) 11, 284, 471, 776, 856
Tru9I TTAA 5 cut(s) 11, 284, 471, 776, 856
TscAI CASTG 1 cut(s) 356
TseI GCWGC 2 cut(s) 74, 167
TspDTI ATGAA 5 cut(s) 360, 395, 500, 546, 729
TspRI CASTG 1 cut(s) 356
Vha464I CTTAAG 1 cut(s) 470
VneI GTGCAC 1 cut(s) 810
VpaK11BI GGWCC 2 cut(s) 31, 402
XapI RAATTY 2 cut(s) 61, 549
XceI RCATGY 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.