Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0002832.1

Orthogroup ID

OG0011400

Physical Location & Seq

Genomic Coordinates Reverse (-)

64619 .. 79462

Gene Structure 14844 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4029 bp

atgccttgctctgcgattagcggcggcggtagcggcggtagtgacggtaatggttctctgcgtaatttcaagctcaacgagtccacgttcttagcctcgctcatgccgaagaaggagatcggcgccgatcgattcgtcgaggctcatccgaattacgacggccgcggcgtcgttattgctatcttcgattctggagtggacccggctgctgctggattacaggttacgtcagatggaaagccgaaaattctagatgttcttgactgcactgggagtggagatgttgatacttcaaaggtggtgaaggctgatgagaacggttgcatacctggagcttcaggggcatctctgcacgtcaactcttcatggaaaaatccttctggtgaatggcacgtgggttataagttggtttacgagctatttactgacacattgacttcccgcttgaagagagaaagaaggaaaaaatgggacgaacaaaaccaagaggaaattgctaaggctgttaagcatcttcatgaatttgaccagaaacacattagagcggaggatgctaacttgaaaagggctcaggaggacctgcaaaacagagttgattacctacaaaagcaagctgatagctatgatgacaaagggcctgtcatagatgctgttgtatggcatgatggagaagtatggagggttgccattgacacacagactcttgaggatgaccctgattgtggaaaactcgctgactttgtgcccctaactaattataggatagaacgaaagtatggcgttttcagcaaattggatgcctgtacgtttgttgttaatgtgtatgatgaagggaagatattgagtattgtaacagattgctctccccatggcacacatgttgctggtattgctactgctttccatgcgaaggagcccatgttgaatggagttgcacctggagcacaagttatatcttgtaaaattggggatgcacgcttaggttcaatggagacaggaactgggctgactcgagctttgattgctgctgtggagcataaatgtgatctgatcaacatgagctatggagaaccagcattactgccaaactatgggcgctttgttgacctcgttaatgaagctgtgaacaagcaccgtctggtatttgtgagtagtgccggcaatagtgggccggcattgagcactgttggggcacctggtggtaccacatcaagcattataggagttggtgcatatgtatctcctgcaatggctgctggtgctcattgtgtagttgaagcccccggtgaaggtcttgaatacacttggtctagccgaggaccaactgccgatggagatcttggtgtctgtattagtgctcctggtgcagctattgctcctgttcctacgtggaccctccaacgacggatgctcatgaatggaacatcaatggcatccccatctgcttgtggtggaattgcactactcataagtgcattgaagtggacagtgcaagttgaaccaaaatttcatgagggtgcgagtaatttagaagaattggttccttttgaggagtgtattgagttatattccactgacaacgcagttgtgagagctcctgagtttctccttcttactcataatggccgtagcttcaacatagttgtaaatcctaccaaccttagtgaaggtctgcactattatgaactatatggtgttaattgcaaagcaccttggcgcggccctcttttcagaatcccaattactataacaaagccaatcactttcataagtcgacctccactatattcattttcgaaattgtcttttttgccaggccacatagaaaggagatttattgaagtacctcttggtgccacatgggttgaagcaaccatgcaaacatcaggatttgatacaacacgaagattttttgttgactcggttcagctttgtcccttacaaagacctcgtaagtgggagagtgttgtaacattctcttcgcctgctgccaaaagcttttcatttcctgttgtgggtggtcagacaatggaattagctatagctcagttttggtcaagtggcataggaagcaacgaaacaacttgtgtggattttgagattgtgtttcatgggattaatgttaataaagaggaactagtgcttgatggaagtgaggcaccagtaagaattgaagctgaagctctactagcatccgagaaacttgcacctgctgctactctaagcaagatcagaattccatatcgaccagttgctgctgaactttgttctcttccaacagatcgtgacaaactaccctcagaaaaacgtattttggcgctgacattaacttacaagttcaaattggaagatggagctgaagtaaaacctcaagttccattacttaacaatcgcatatatgatactaaatttgagtctcagttttatatgatctctgatgcaaacaagcgtgtatatgcaacgggtgaagcttatccaagctcttcaaaactgcccaaaggcgaatacactttacagctatatcttaggcacgaaaacatgcaatatctggaaaaattgaagcagctggttttgttcattgaaagaaaattggaagagaaggatgtaatgcgtttgagttttttctctcaaccggatggccctgtgatgggaaatggtgcttataagtcctcagttttagttccagggaaaaaagaagcaatctatttgggcccaccatccaaagacaagctcctaaagttttctccgcaaggatctgttttgcttggagctatctcctatgggaagctatcatatgctgaaaaaggacaagggaaagatccacaaaagaacccggtgtcttatcagatttcttatattgtgccaccgaataagatggatgaagacaaaggaaaaggttcttctgcttccaccaaagctgtttctgaacgtttagaagaagaggttcgagatgcgaagattaaagttcttacaagcctcaaacatgacaatgaagaagaacgctcagagtggaacaaattatctacctctcttaagtctgaatacccaaaacacaccccattgcttgcaaagattttggaaggtttgctttctcgaaacagtgtcgaggacaaaatctgtcatgacaaagaggttattgatgcagcaaatgaggttgttgatagtattgacagagatgagctagcaaaatttttttcacttagaactgatcccgaagatgaggaggcagagaaaaataaaaagaagatggagaccactcgtgatcagttagcagaggcattgtaccaaaaaggactagcactggcagatattttgtctttacagggggataagccttcggcgtcggaagtaactgaaggttcaaaagaggcagacaagactgctgactctggaccaggctctggtggcctgcttgatcgctttgaagacacttttaaagaacttcaaaaatgggtcgaagtgaaatcctctaaatacggaatcctttcagtaacacgtgagaaacatcatggaaggcttggaacagcactcaaggtcttgaatgacataattcaagagaatacggagcctccgaagaagaagctttacgaagaaaagctgtctctgcttgaagagatcgggtggcagcacttggtgacatatgagagacaatggatgcattctggtcttactggagagtcacttttagtgaccaaaaatcctggttatgccgtttactcaggattaacatgcaaacagggaaccactgcaagtggcctagtggttcttgttgtagtctggtcttactggagagtcacttttagtgaccaaaaatcctggttatgccgtttactcaggattaacatgcaaacaaggaaccactgcaagtggcctagtggttattgtctagttgggtgtgctccccaacctagattcgaatcccgaagccgtcaaagtggccaggcactgtgctgcaatgcacagttggagcatttcacatgcgccgaaggggtttatcttgggcctaggaagcctttgggctccccttaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004175 GO:0004177 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0008240 GO:0009507 GO:0009536 GO:0009987 GO:0016020 GO:0016567 GO:0016604 GO:0016787 GO:0017171 GO:0019538 GO:0022626 GO:0031090 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044451 GO:0044464 GO:0070011 GO:0070013 GO:0070647 GO:0071704 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S8 TPPII

Protein Analysis

1342

Amino Acids

147.67

Weight (kDa)

6.09

Isoelectric Point (pI)

38.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S8	PF00082	54 - 495	2.3e-42	Subtilase family
TPPII_Ig-like-1	PF21223	493 - 583	1.3e-15	Tripeptidyl-peptidase II, first Ig-like domain
TPPII_GBD	PF21316	603 - 687	2e-15	TPPII, galactose-binding domain-like
TPPII	PF12580	729 - 913	1.2e-59	Tripeptidyl peptidase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20850
fragaria_vesca	FvH4_7g29630 FvH4_7g29630 FvH4_7g29630
malus_domestica	MD01G1203100.v1.1 MD07G1272600.v1.1
prunus_persica	Prupe.2G295900_v2.0.a1 Prupe.2G295900_v2.0.a1
pyrus_communis	pycom01g21240 pycom07g24900
rosa_chinensis	RchiOBHm_Chr1g0377641
rosa_laevigata	RLG00000026509
rosa_multiflora	Rmu_sc0002832.1_g000013
rosa_roxburghii	Rroxscaffold_4G00281150
rosa_rugosa	Rorug01G0403900
rosa_samantha	Rh1AG428700 Rh1BG380700 Rh1CG394700 Rh1DG411700
rosa_wichuraiana	Rw1G037000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	402, 2664
AarI	CACCTGC	1 cut(s)	2218
Acc36I	ACCTGC	2 cut(s)	588, 2218
Acc65I	GGTACC	1 cut(s)	1202
AccB1I	GGYRCC	5 cut(s)	122, 1192, 1202, 1853, 2158
AccB7I	CCANNNNNTGG	2 cut(s)	1091, 3401
AccBSI	CCGCTC	1 cut(s)	545
AccI	GTMKAC	1 cut(s)	1774
AccII	CGCG	2 cut(s)	165, 1719
AclI	AACGTT	1 cut(s)	2929
AclWI	GGATC	3 cut(s)	2761, 2813, 3203
AcoI	YGGCCR	3 cut(s)	160, 1624, 3937
AcsI	RAATTY	6 cut(s)	246, 521, 1505, 2235, 2408, 3188
AcuI	CTGAAG	4 cut(s)	321, 2199, 2379, 3375
AcvI	CACGTG	2 cut(s)	394, 3497
AcyI	GRCGYC	3 cut(s)	123, 168, 3341
AdeI	CACNNNGTG	2 cut(s)	1199, 3634
AfaI	GTAC	4 cut(s)	805, 1204, 1845, 3284
AfiI	CCNNNNNNNGG	9 cut(s)	722, 910, 1091, 1289, 1718, 2015, 2647, 2648, 3401
AflII	CTTAAG	1 cut(s)	3032
AflIII	ACRYGT	2 cut(s)	877, 3494
AhlI	ACTAGT	1 cut(s)	2137
AjiI	CACGTC	1 cut(s)	355
AjuI	GAANNNNNNNTTGG	2 cut(s)	1833, 1865
Alw21I	GWGCWC	6 cut(s)	946, 1184, 1264, 1360, 1597, 3901
Alw26I	GTCTC	5 cut(s)	986, 2421, 3245, 3606, 3640
AlwI	GGATC	3 cut(s)	2761, 2813, 3203
AlwNI	CAGNNNCTG	4 cut(s)	1001, 2255, 3401, 3946
Ama87I	CYCGRG	1 cut(s)	1011
ApaI	GGGCCC	1 cut(s)	2714
ApoI	RAATTY	6 cut(s)	246, 521, 1505, 2235, 2408, 3188
ArsI	GACNNNNNNTTYG	4 cut(s)	624, 656, 1000, 1032
AseI	ATTAAT	1 cut(s)	2118
Asp700I	GAANNNNTTC	4 cut(s)	1539, 2896, 2943, 3484
Asp718I	GGTACC	1 cut(s)	1202
AspA2I	CCTAGG	1 cut(s)	4004
AspLEI	GCGC	5 cut(s)	125, 1098, 1719, 2320, 3983
AsuC2I	CCSGG	3 cut(s)	203, 1284, 2834
AsuHPI	GGTGA	5 cut(s)	313, 395, 1298, 2477, 3646
AsuII	TTCGAA	2 cut(s)	1796, 3915
AsuNHI	GCTAGC	1 cut(s)	3181
AvaI	CYCGRG	1 cut(s)	1011
AvaII	GGWCC	5 cut(s)	199, 577, 1319, 1392, 3392
AvrII	CCTAGG	1 cut(s)	4004
BaeGI	GKGCMC	3 cut(s)	747, 1195, 2714
BalI	TGGCCA	1 cut(s)	3939
BanI	GGYRCC	5 cut(s)	122, 1192, 1202, 1853, 2158
BanII	GRGCYC	5 cut(s)	571, 918, 1597, 2714, 4022
BarI	GAAGNNNNNNTAC	2 cut(s)	3569, 3601
BauI	CACGAG	1 cut(s)	3258
BbrPI	CACGTG	2 cut(s)	394, 3497
BbsI	GAAGAC	2 cut(s)	2889, 3432
Bbv12I	GWGCWC	6 cut(s)	946, 1184, 1264, 1360, 1597, 3901
BceAI	ACGGC	5 cut(s)	175, 1611, 3695, 3810, 3912
BcgI	CGANNNNNNTGC	2 cut(s)	2186, 2220
BclI	TGATCA	2 cut(s)	1050, 3262
BcnI	CCSGG	3 cut(s)	203, 1284, 2834
BcoDI	GTCTC	5 cut(s)	986, 2421, 3245, 3606, 3640
BcuI	ACTAGT	1 cut(s)	2137
BfmI	CTRYAG	1 cut(s)	2040
BfoI	RGCGCY	3 cut(s)	126, 1099, 2321
BfrI	CTTAAG	1 cut(s)	3032
BfuAI	ACCTGC	2 cut(s)	588, 2218
BglII	AGATCT	1 cut(s)	1336
BlnI	CCTAGG	1 cut(s)	4004
Bme18I	GGWCC	5 cut(s)	199, 577, 1319, 1392, 3392
BmeT110I	CYCGRG	1 cut(s)	1011
BmgBI	CACGTC	1 cut(s)	355
BmrI	ACTGGG	2 cut(s)	279, 1011
BmtI	GCTAGC	1 cut(s)	3185
BmuI	ACTGGG	2 cut(s)	279, 1011
BpiI	GAAGAC	2 cut(s)	2889, 3432
BpmI	CTGGAG	5 cut(s)	213, 351, 960, 3693, 3808
Bpu10I	CCTNAGC	3 cut(s)	498, 570, 979
Bpu14I	TTCGAA	2 cut(s)	1796, 3915
BpuEI	CTTGAG	3 cut(s)	725, 2355, 3515
BpuMI	CCSGG	3 cut(s)	203, 1284, 2834
Bsa29I	ATCGAT	1 cut(s)	130
BsaAI	YACGTR	3 cut(s)	394, 1389, 3497
BsaBI	GATNNNNATC	2 cut(s)	1335, 3916
BsaHI	GRCGYC	3 cut(s)	123, 168, 3341
BsaI	GGTCTC	1 cut(s)	3245
BsaJI	CCNNGG	7 cut(s)	163, 868, 1282, 1315, 1712, 2684, 4004
BsaWI	WCCGGW	1 cut(s)	2632
BsaXI	ACNNNNNCTCC	8 cut(s)	905, 933, 935, 963, 2346, 2376, 3553, 3583
Bsc4I	CCNNNNNNNGG	9 cut(s)	722, 910, 1091, 1289, 1718, 2015, 2647, 2648, 3401
Bse118I	RCCGGY	2 cut(s)	1157, 1171
Bse1I	ACTGG	7 cut(s)	274, 1006, 2162, 2249, 3306, 3676, 3791
Bse3DI	GCAATG	3 cut(s)	1254, 3059, 3961
Bse8I	GATNNNNATC	2 cut(s)	1335, 3916
BseCI	ATCGAT	1 cut(s)	130
BseDI	CCNNGG	7 cut(s)	163, 868, 1282, 1315, 1712, 2684, 4004
BseJI	GATNNNNATC	2 cut(s)	1335, 3916
BseLI	CCNNNNNNNGG	9 cut(s)	722, 910, 1091, 1289, 1718, 2015, 2647, 2648, 3401
BseMI	GCAATG	3 cut(s)	1254, 3059, 3961
BseMII	CTCAG	9 cut(s)	584, 1590, 2060, 2313, 2432, 2685, 3018, 3732, 3847
BseNI	ACTGG	7 cut(s)	274, 1006, 2162, 2249, 3306, 3676, 3791
BseRI	GAGGAG	2 cut(s)	1565, 3236
BseSI	GKGCMC	3 cut(s)	747, 1195, 2714
BseX3I	CGGCCG	1 cut(s)	160
BsgI	GTGCAG	4 cut(s)	250, 335, 1386, 1658
Bsh1236I	CGCG	2 cut(s)	165, 1719
Bsh1285I	CGRYCG	2 cut(s)	130, 163
BshNI	GGYRCC	5 cut(s)	122, 1192, 1202, 1853, 2158
BshVI	ATCGAT	1 cut(s)	130
BsiEI	CGRYCG	2 cut(s)	130, 163
BsiHKAI	GWGCWC	6 cut(s)	946, 1184, 1264, 1360, 1597, 3901
BsiHKCI	CYCGRG	1 cut(s)	1011
BsiSI	CCGG	6 cut(s)	203, 1158, 1172, 1284, 2633, 2834
BslFI	GGGAC	2 cut(s)	485, 1920
BslI	CCNNNNNNNGG	9 cut(s)	722, 910, 1091, 1289, 1718, 2015, 2647, 2648, 3401
BsmAI	GTCTC	5 cut(s)	986, 2421, 3245, 3606, 3640
BsmFI	GGGAC	2 cut(s)	485, 1920
BsmI	GAATGC	1 cut(s)	3658
Bso31I	GGTCTC	1 cut(s)	3245
BsoBI	CYCGRG	1 cut(s)	1011
Bsp119I	TTCGAA	2 cut(s)	1796, 3915
Bsp120I	GGGCCC	1 cut(s)	2710
Bsp19I	CCATGG	1 cut(s)	868
BspCNI	CTCAG	9 cut(s)	583, 1591, 2059, 2312, 2431, 2684, 3017, 3731, 3846
BspDI	ATCGAT	1 cut(s)	130
BspFNI	CGCG	2 cut(s)	165, 1719
BspHI	TCATGA	4 cut(s)	517, 1413, 1510, 3121
BspMI	ACCTGC	2 cut(s)	588, 2218
BspOI	GCTAGC	1 cut(s)	3185
BspPI	GGATC	3 cut(s)	2761, 2813, 3203
BspQI	GCTCTTC	1 cut(s)	2488
BspT104I	TTCGAA	2 cut(s)	1796, 3915
BspT107I	GGYRCC	5 cut(s)	122, 1192, 1202, 1853, 2158
BspTI	CTTAAG	1 cut(s)	3032
BspTNI	GGTCTC	1 cut(s)	3245
BsrBI	CCGCTC	1 cut(s)	545
BsrDI	GCAATG	3 cut(s)	1254, 3059, 3961
BsrFI	RCCGGY	2 cut(s)	1157, 1171
BsrI	ACTGG	7 cut(s)	274, 1006, 2162, 2249, 3306, 3676, 3791
BssAI	RCCGGY	2 cut(s)	1157, 1171
BssECI	CCNNGG	7 cut(s)	163, 868, 1282, 1315, 1712, 2684, 4004
BssNI	GRCGYC	3 cut(s)	123, 168, 3341
BssSI	CACGAG	1 cut(s)	3258
BssT1I	CCWWGG	3 cut(s)	868, 1712, 4004
Bst2BI	CACGAG	1 cut(s)	3258
Bst4CI	ACNGT	8 cut(s)	47, 320, 1136, 1186, 1489, 3101, 3948, 3963
Bst6I	CTCTTC	8 cut(s)	367, 443, 1984, 2277, 2488, 2589, 2934, 3606
BstACI	GRCGYC	3 cut(s)	123, 168, 3341
BstAFI	CTTAAG	1 cut(s)	3032
BstAPI	GCANNNNNTGC	3 cut(s)	1253, 1373, 2213
BstBAI	YACGTR	3 cut(s)	394, 1389, 3497
BstBI	TTCGAA	2 cut(s)	1796, 3915
BstC8I	GCNNGC	8 cut(s)	612, 976, 1159, 1173, 1986, 3066, 3183, 3410
BstDSI	CCRYGG	2 cut(s)	163, 868
BstFNI	CGCG	2 cut(s)	165, 1719
BstH2I	RGCGCY	3 cut(s)	126, 1099, 2321
BstHHI	GCGC	5 cut(s)	125, 1098, 1719, 2320, 3983
BstMAI	GTCTC	5 cut(s)	986, 2421, 3245, 3606, 3640
BstMCI	CGRYCG	2 cut(s)	130, 163
BstNSI	RCATGY	5 cut(s)	881, 2542, 3732, 3847, 3981
BstSFI	CTRYAG	1 cut(s)	2040
BstSLI	GKGCMC	3 cut(s)	747, 1195, 2714
BstUI	CGCG	2 cut(s)	165, 1719
BstV2I	GAAGAC	2 cut(s)	2889, 3432
BstX2I	RGATCY	3 cut(s)	1336, 2753, 2818
BstYI	RGATCY	3 cut(s)	1336, 2753, 2818
BstZI	CGGCCG	1 cut(s)	160
Bsu15I	ATCGAT	1 cut(s)	130
BsuTUI	ATCGAT	1 cut(s)	130
BtgI	CCRYGG	2 cut(s)	163, 868
BtrI	CACGTC	1 cut(s)	355
BtsI	GCAGTG	2 cut(s)	3744, 3859
BtsIMutI	CAGTG	9 cut(s)	267, 1182, 1494, 1572, 3106, 3299, 3744, 3859, 3944
BveI	ACCTGC	2 cut(s)	588, 2218
Cac8I	GCNNGC	8 cut(s)	612, 976, 1159, 1173, 1986, 3066, 3183, 3410
CaiI	CAGNNNCTG	4 cut(s)	1001, 2255, 3401, 3946
CciI	TCATGA	4 cut(s)	517, 1413, 1510, 3121
CfoI	GCGC	5 cut(s)	125, 1098, 1719, 2320, 3983
Cfr10I	RCCGGY	2 cut(s)	1157, 1171
Cfr42I	CCGCGG	1 cut(s)	166
ClaI	ATCGAT	1 cut(s)	130
CseI	GACGC	2 cut(s)	157, 3330
CsiI	ACCWGGT	1 cut(s)	1195
Csp6I	GTAC	4 cut(s)	804, 1203, 1844, 3283
CspCI	CAANNNNNGTGG	4 cut(s)	1846, 1881, 2070, 2105
CviQI	GTAC	4 cut(s)	804, 1203, 1844, 3283
DinI	GGCGCC	1 cut(s)	124
DraI	TTTAAA	1 cut(s)	3436
DraIII	CACNNNGTG	2 cut(s)	1199, 3634
EaeI	YGGCCR	3 cut(s)	160, 1624, 3937
EagI	CGGCCG	1 cut(s)	160
Eam1104I	CTCTTC	8 cut(s)	367, 443, 1984, 2277, 2488, 2589, 2934, 3606
EarI	CTCTTC	8 cut(s)	367, 443, 1984, 2277, 2488, 2589, 2934, 3606
Ecl136II	GAGCTC	1 cut(s)	1595
EclXI	CGGCCG	1 cut(s)	160
Eco130I	CCWWGG	3 cut(s)	868, 1712, 4004
Eco24I	GRGCYC	5 cut(s)	571, 918, 1597, 2714, 4022
Eco31I	GGTCTC	1 cut(s)	3245
Eco47I	GGWCC	5 cut(s)	199, 577, 1319, 1392, 3392
Eco52I	CGGCCG	1 cut(s)	160
Eco53kI	GAGCTC	1 cut(s)	1595
Eco57I	CTGAAG	4 cut(s)	321, 2199, 2379, 3375
Eco72I	CACGTG	2 cut(s)	394, 3497
Eco88I	CYCGRG	1 cut(s)	1011
EcoICRI	GAGCTC	1 cut(s)	1595
EcoO109I	RGGNCCY	2 cut(s)	577, 635
EcoRI	GAATTC	1 cut(s)	2235
EcoT14I	CCWWGG	3 cut(s)	868, 1712, 4004
EcoT22I	ATGCAT	1 cut(s)	3660
EcoT38I	GRGCYC	5 cut(s)	571, 918, 1597, 2714, 4022
EgeI	GGCGCC	1 cut(s)	124
EheI	GGCGCC	1 cut(s)	124
ErhI	CCWWGG	3 cut(s)	868, 1712, 4004
FalI	AAGNNNNNCTT	4 cut(s)	1833, 1865, 3072, 3104
FaqI	GGGAC	2 cut(s)	485, 1920
FauI	CCCGC	1 cut(s)	449
FauNDI	CATATG	3 cut(s)	1234, 2794, 3640
FbaI	TGATCA	2 cut(s)	1050, 3262
FblI	GTMKAC	1 cut(s)	1774
FriOI	GRGCYC	5 cut(s)	571, 918, 1597, 2714, 4022
GlaI	GCGC	5 cut(s)	124, 1097, 1718, 2319, 3982
GsuI	CTGGAG	5 cut(s)	213, 351, 960, 3693, 3808
HaeII	RGCGCY	3 cut(s)	126, 1099, 2321
HapII	CCGG	6 cut(s)	203, 1158, 1172, 1284, 2633, 2834
HgaI	GACGC	2 cut(s)	157, 3330
HhaI	GCGC	5 cut(s)	125, 1098, 1719, 2320, 3983
Hin1I	GRCGYC	3 cut(s)	123, 168, 3341
Hin6I	GCGC	5 cut(s)	123, 1096, 1717, 2318, 3981
HinP1I	GCGC	5 cut(s)	123, 1096, 1717, 2318, 3981
HincII	GTYRAC	4 cut(s)	358, 1105, 1775, 1918
HindII	GTYRAC	4 cut(s)	358, 1105, 1775, 1918
HindIII	AAGCTT	3 cut(s)	1996, 2469, 3581
HpaII	CCGG	6 cut(s)	203, 1158, 1172, 1284, 2633, 2834
HphI	GGTGA	5 cut(s)	313, 395, 1298, 2477, 3646
Hpy99I	CGWCG	5 cut(s)	140, 161, 173, 1407, 3346
HpyCH4III	ACNGT	8 cut(s)	47, 320, 1136, 1186, 1489, 3101, 3948, 3963
HpyCH4IV	ACGT	9 cut(s)	86, 227, 354, 393, 806, 1388, 2308, 2929, 3496
HpySE526I	ACGT	9 cut(s)	86, 227, 354, 393, 806, 1388, 2308, 2929, 3496
Hsp92I	GRCGYC	3 cut(s)	123, 168, 3341
HspAI	GCGC	5 cut(s)	123, 1096, 1717, 2318, 3981
KasI	GGCGCC	1 cut(s)	122
KpnI	GGTACC	1 cut(s)	1206
KroI	GCCGGC	2 cut(s)	1157, 1171
KroNI	GCCGGC	2 cut(s)	1159, 1173
Ksp22I	TGATCA	2 cut(s)	1050, 3262
KspI	CCGCGG	1 cut(s)	166
LguI	GCTCTTC	1 cut(s)	2488
MabI	ACCWGGT	1 cut(s)	1195
MaeII	ACGT	9 cut(s)	86, 227, 354, 393, 806, 1388, 2308, 2929, 3496
MbiI	CCGCTC	1 cut(s)	545
MflI	RGATCY	3 cut(s)	1336, 2753, 2818
MlsI	TGGCCA	1 cut(s)	3939
MluNI	TGGCCA	1 cut(s)	3939
Mly113I	GGCGCC	1 cut(s)	123
MlyI	GAGTC	8 cut(s)	89, 694, 1003, 1913, 2423, 3380, 3686, 3801
MmeI	TCCRAC	4 cut(s)	1423, 2300, 3324, 3945
Mox20I	TGGCCA	1 cut(s)	3939
Mph1103I	ATGCAT	1 cut(s)	3660
MroNI	GCCGGC	2 cut(s)	1157, 1171
MroXI	GAANNNNTTC	4 cut(s)	1539, 2896, 2943, 3484
MscI	TGGCCA	1 cut(s)	3939
MslI	CAYNNNNRTG	6 cut(s)	516, 1041, 1480, 1515, 1680, 2988
Msp20I	TGGCCA	1 cut(s)	3939
MspA1I	CMGCKG	2 cut(s)	165, 2566
MspCI	CTTAAG	1 cut(s)	3032
MspI	CCGG	6 cut(s)	203, 1158, 1172, 1284, 2633, 2834
Mva1269I	GAATGC	1 cut(s)	3658
MvnI	CGCG	2 cut(s)	165, 1719
NaeI	GCCGGC	2 cut(s)	1159, 1173
NarI	GGCGCC	1 cut(s)	123
NciI	CCSGG	3 cut(s)	203, 1284, 2834
NcoI	CCATGG	1 cut(s)	868
NdeI	CATATG	3 cut(s)	1234, 2794, 3640
NgoMIV	GCCGGC	2 cut(s)	1157, 1171
NheI	GCTAGC	1 cut(s)	3181
NmeAIII	GCCGAG	1 cut(s)	1340
NmuCI	GTSAC	7 cut(s)	41, 2285, 3634, 3678, 3688, 3793, 3803
NsiI	ATGCAT	1 cut(s)	3660
NspI	RCATGY	5 cut(s)	881, 2542, 3732, 3847, 3981
NspV	TTCGAA	2 cut(s)	1796, 3915
PaeR7I	CTCGAG	1 cut(s)	1011
PagI	TCATGA	4 cut(s)	517, 1413, 1510, 3121
PaqCI	CACCTGC	1 cut(s)	2218
PciI	ACATGT	1 cut(s)	877
PciSI	GCTCTTC	1 cut(s)	2488
PcsI	WCGNNNNNNNCGW	3 cut(s)	104, 165, 3569
PctI	GAATGC	1 cut(s)	3658
PdiI	GCCGGC	2 cut(s)	1159, 1173
PdmI	GAANNNNTTC	4 cut(s)	1539, 2896, 2943, 3484
PfeI	GAWTC	6 cut(s)	132, 188, 1734, 3480, 3912, 3917
PflMI	CCANNNNNTGG	2 cut(s)	1091, 3401
Ple19I	CGATCG	1 cut(s)	130
PleI	GAGTC	8 cut(s)	88, 694, 1003, 1913, 2422, 3380, 3685, 3800
PluTI	GGCGCC	1 cut(s)	126
PmaCI	CACGTG	2 cut(s)	394, 3497
PmlI	CACGTG	2 cut(s)	394, 3497
PpsI	GAGTC	8 cut(s)	88, 694, 1003, 1913, 2422, 3380, 3685, 3800
Ppu21I	YACGTR	3 cut(s)	394, 1389, 3497
PpuMI	RGGWCCY	1 cut(s)	577
PscI	ACATGT	1 cut(s)	877
PshBI	ATTAAT	1 cut(s)	2118
PsiI	TTATAA	2 cut(s)	402, 2664
Psp124BI	GAGCTC	1 cut(s)	1597
Psp1406I	AACGTT	1 cut(s)	2929
Psp5II	RGGWCCY	1 cut(s)	577
PspCI	CACGTG	2 cut(s)	394, 3497
PspOMI	GGGCCC	1 cut(s)	2710
PspPPI	RGGWCCY	1 cut(s)	577
PspXI	VCTCGAGB	1 cut(s)	1011
PsrI	GAACNNNNNNTAC	2 cut(s)	1062, 1094
PstNI	CAGNNNCTG	4 cut(s)	1001, 2255, 3401, 3946
PsuI	RGATCY	3 cut(s)	1336, 2753, 2818
PvuI	CGATCG	1 cut(s)	130
PvuII	CAGCTG	1 cut(s)	2566
RsaI	GTAC	4 cut(s)	805, 1204, 1845, 3284
RsaNI	GTAC	4 cut(s)	804, 1203, 1844, 3283
RseI	CAYNNNNRTG	6 cut(s)	516, 1041, 1480, 1515, 1680, 2988
SacI	GAGCTC	1 cut(s)	1597
SacII	CCGCGG	1 cut(s)	166
SalI	GTCGAC	1 cut(s)	1773
SapI	GCTCTTC	1 cut(s)	2488
SchI	GAGTC	8 cut(s)	89, 694, 1003, 1913, 2423, 3380, 3686, 3801
SexAI	ACCWGGT	1 cut(s)	1195
SfcI	CTRYAG	1 cut(s)	2040
SfoI	GGCGCC	1 cut(s)	124
Sfr274I	CTCGAG	1 cut(s)	1011
Sfr303I	CCGCGG	1 cut(s)	166
SfuI	TTCGAA	2 cut(s)	1796, 3915
SgrBI	CCGCGG	1 cut(s)	166
SinI	GGWCC	5 cut(s)	199, 577, 1319, 1392, 3392
SlaI	CTCGAG	1 cut(s)	1011
SmiMI	CAYNNNNRTG	6 cut(s)	516, 1041, 1480, 1515, 1680, 2988
SmlI	CTYRAG	5 cut(s)	704, 1011, 2370, 3032, 3530
SmoI	CTYRAG	5 cut(s)	704, 1011, 2370, 3032, 3530
SpeI	ACTAGT	1 cut(s)	2137
SspDI	GGCGCC	1 cut(s)	122
SstI	GAGCTC	1 cut(s)	1597
StyI	CCWWGG	3 cut(s)	868, 1712, 4004
TaaI	ACNGT	8 cut(s)	47, 320, 1136, 1186, 1489, 3101, 3948, 3963
TaiI	ACGT	9 cut(s)	89, 230, 357, 396, 809, 1391, 2311, 2932, 3499
TauI	GCSGC	6 cut(s)	24, 27, 36, 165, 168, 1722
TfiI	GAWTC	6 cut(s)	132, 188, 1734, 3480, 3912, 3917
TscAI	CASTG	9 cut(s)	274, 1189, 1494, 1579, 3106, 3306, 3751, 3866, 3951
TseFI	GTSAC	7 cut(s)	41, 2285, 3634, 3678, 3688, 3793, 3803
Tsp45I	GTSAC	7 cut(s)	41, 2285, 3634, 3678, 3688, 3793, 3803
TspGWI	ACGGA	3 cut(s)	1420, 3492, 3578
TspRI	CASTG	9 cut(s)	274, 1189, 1494, 1579, 3106, 3306, 3751, 3866, 3951
Van91I	CCANNNNNTGG	2 cut(s)	1091, 3401
Vha464I	CTTAAG	1 cut(s)	3032
VpaK11BI	GGWCC	5 cut(s)	199, 577, 1319, 1392, 3392
VspI	ATTAAT	1 cut(s)	2118
XapI	RAATTY	6 cut(s)	246, 521, 1505, 2235, 2408, 3188
XbaI	TCTAGA	1 cut(s)	250
XceI	RCATGY	5 cut(s)	881, 2542, 3732, 3847, 3981
XhoI	CTCGAG	1 cut(s)	1011
XmaJI	CCTAGG	1 cut(s)	4004
XmiI	GTMKAC	1 cut(s)	1774
XmnI	GAANNNNTTC	4 cut(s)	1539, 2896, 2943, 3484
Zsp2I	ATGCAT	1 cut(s)	3660

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.