Rmu_sc0003272.1_g000010

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003272.1
OG0018962
Physical Location & Seq
Forward (+)
40733 .. 43150
2418 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 921 bp
atggtcaacgagacggtggttgaagctgtgacgccgggcaagagtggtagttgtggtgtgcttgttgagagggaacaaggtgagagctttgaggagtgtgcaacaagagaagtgaaggaagaaactggtttagacattgataagttacagtttttgacagtcacaaacaacctgttcttagacaaagccaaaccataccattatgtgagaaggaaaacccatagtagttcgtttctctcccggccgagcaccactggcgatcctgcagttgcgttccggcccgggaggggatggtgttgttggttagtggtgggtcttcagggcagagatgcggtccaagggaggagggtgtgggtagatctctcgatcggtccctctcaaatcaggggcgacagcggttgtggggatgcagcaacttgtttctggccggcggtgggagttggcgtcatcaacgaagcgatctgggttatggaggatgcgggcaactcgcggcagcagggatcctggttgggctctgtttctgtctggtttctggatctggttgcggtgtcaattgggtgtagaatttgtctgcatattggcatggtggagcgacggtatcgggaggcggcgaggccgtggggtggcgttgatggtgcttgccggtggcgggattgggtttctgcattggtggagtactgctcaaggaagtgggtgttgggtcgggcccctgtttttgggccttggagcacttttggacacggtgttgaagaggacgctgtcttcttggaggttgattgtaaggttctactagaaatttcggggtcaaatgttcaaagtagccttggaaatgggtttagtgtttggaccctttggcctgatggtctctatgaccgtggtttcttggggtttaggtttatgtccccccctttgtacgcgtga

Protein Analysis

306

Amino Acids

33.81

Weight (kDa)

5.69

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 25 - 89 3.5e-08 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 490, 917
AciI CCGC 8 cut(s) 332, 396, 431, 479, 490, 545, 608, 649
AclWI GGATC 4 cut(s) 254, 495, 508, 543
AcoI YGGCCR 2 cut(s) 242, 425
AcsI RAATTY 2 cut(s) 564, 795
AcuI CTGAAG 1 cut(s) 302
AcyI GRCGYC 2 cut(s) 32, 444
AfaI GTAC 2 cut(s) 677, 914
AfiI CCNNNNNNNGG 5 cut(s) 287, 434, 623, 649, 716
AflIII ACRYGT 1 cut(s) 915
AgsI TTSAA 3 cut(s) 23, 749, 815
AjnI CCWGG 1 cut(s) 503
AjuI GAANNNNNNNTTGG 2 cut(s) 680, 712
AluBI AGCT 2 cut(s) 26, 87
AluI AGCT 2 cut(s) 26, 87
Alw21I GWGCWC 2 cut(s) 251, 731
Alw26I GTCTC 2 cut(s) 5, 869
AlwI GGATC 4 cut(s) 254, 495, 508, 543
Ama87I CYCGRG 1 cut(s) 281
AoxI GGCC 7 cut(s) 242, 278, 425, 614, 705, 719, 854
ApaI GGGCCC 1 cut(s) 709
ApeKI GCWGC 2 cut(s) 410, 493
ApoI RAATTY 2 cut(s) 564, 795
AspS9I GGNCC 7 cut(s) 279, 334, 371, 705, 706, 719, 846
AsuC2I CCSGG 4 cut(s) 36, 241, 282, 283
AsuHPI GGTGA 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 3 cut(s) 334, 371, 846
BaeGI GKGCMC 1 cut(s) 709
BamHI GGATCC 1 cut(s) 500
BanII GRGCYC 2 cut(s) 515, 709
BbsI GAAGAC 2 cut(s) 308, 754
Bbv12I GWGCWC 2 cut(s) 251, 731
BbvI GCAGC 2 cut(s) 422, 505
BccI CCATC 3 cut(s) 285, 626, 854
BceAI ACGGC 1 cut(s) 601
BciT130I CCWGG 1 cut(s) 505
BcnI CCSGG 4 cut(s) 36, 241, 282, 283
BcoDI GTCTC 2 cut(s) 5, 869
BfaI CTAG 1 cut(s) 791
BfmI CTRYAG 1 cut(s) 264
BglII AGATCT 1 cut(s) 358
BisI GCNGC 4 cut(s) 411, 491, 494, 609
BlsI GCNGC 4 cut(s) 412, 492, 495, 610
BmcAI AGTACT 1 cut(s) 677
Bme1390I CCNGG 5 cut(s) 36, 241, 282, 283, 505
Bme18I GGWCC 3 cut(s) 334, 371, 846
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 7 cut(s) 279, 334, 371, 705, 706, 719, 846
BmiI GGNNCC 5 cut(s) 373, 502, 707, 708, 848
BmrFI CCNGG 5 cut(s) 36, 241, 282, 283, 505
BmsI GCATC 3 cut(s) 319, 397, 466
BpiI GAAGAC 2 cut(s) 308, 754
BplI GAGNNNNNCTC 2 cut(s) 665, 697
BpuEI CTTGAG 1 cut(s) 667
BpuMI CCSGG 4 cut(s) 36, 241, 282, 283
BsaHI GRCGYC 2 cut(s) 32, 444
BsaI GGTCTC 1 cut(s) 869
BsaJI CCNNGG 6 cut(s) 281, 337, 617, 722, 823, 874
BsaXI ACNNNNNCTCC 4 cut(s) 429, 459, 581, 611
Bsc4I CCNNNNNNNGG 5 cut(s) 287, 434, 623, 649, 716
Bse118I RCCGGY 2 cut(s) 427, 642
Bse1I ACTGG 2 cut(s) 130, 259
BseBI CCWGG 1 cut(s) 505
BseDI CCNNGG 6 cut(s) 281, 337, 617, 722, 823, 874
BseGI GGATG 3 cut(s) 296, 412, 481
BseLI CCNNNNNNNGG 5 cut(s) 287, 434, 623, 649, 716
BseNI ACTGG 2 cut(s) 130, 259
BseRI GAGGAG 2 cut(s) 107, 358
BseSI GKGCMC 1 cut(s) 709
BseX3I CGGCCG 1 cut(s) 242
BseXI GCAGC 2 cut(s) 422, 505
Bsh1236I CGCG 2 cut(s) 490, 917
Bsh1285I CGRYCG 2 cut(s) 245, 369
BshFI GGCC 7 cut(s) 244, 280, 427, 616, 707, 721, 856
BsiEI CGRYCG 2 cut(s) 245, 369
BsiHKAI GWGCWC 2 cut(s) 251, 731
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 6 cut(s) 35, 241, 277, 282, 428, 643
BslFI GGGAC 2 cut(s) 357, 886
BslI CCNNNNNNNGG 5 cut(s) 287, 434, 623, 649, 716
BsmAI GTCTC 2 cut(s) 5, 869
BsmBI CGTCTC 1 cut(s) 5
BsmFI GGGAC 2 cut(s) 357, 886
BsnI GGCC 7 cut(s) 244, 280, 427, 616, 707, 721, 856
Bso31I GGTCTC 1 cut(s) 869
BsoBI CYCGRG 1 cut(s) 281
Bsp120I GGGCCC 1 cut(s) 705
Bsp1286I GDGCHC 4 cut(s) 251, 515, 709, 731
Bsp143I GATC 6 cut(s) 259, 358, 366, 459, 500, 535
BspACI CCGC 8 cut(s) 332, 396, 431, 479, 490, 545, 608, 649
BspANI GGCC 7 cut(s) 244, 280, 427, 616, 707, 721, 856
BspFNI CGCG 2 cut(s) 490, 917
BspLI GGNNCC 5 cut(s) 373, 502, 707, 708, 848
BspMAI CTGCAG 1 cut(s) 268
BspPI GGATC 4 cut(s) 254, 495, 508, 543
BspTNI GGTCTC 1 cut(s) 869
BsrFI RCCGGY 2 cut(s) 427, 642
BsrI ACTGG 2 cut(s) 130, 259
BssAI RCCGGY 2 cut(s) 427, 642
BssECI CCNNGG 6 cut(s) 281, 337, 617, 722, 823, 874
BssMI GATC 6 cut(s) 259, 358, 366, 459, 500, 535
BssNI GRCGYC 2 cut(s) 32, 444
BssT1I CCWWGG 3 cut(s) 337, 722, 823
Bst2UI CCWGG 1 cut(s) 505
Bst4CI ACNGT 6 cut(s) 16, 150, 160, 597, 743, 875
Bst6I CTCTTC 1 cut(s) 744
BstACI GRCGYC 2 cut(s) 32, 444
BstC8I GCNNGC 3 cut(s) 429, 481, 640
BstDEI CTNAG 1 cut(s) 178
BstDSI CCRYGG 2 cut(s) 617, 874
BstF5I GGATG 3 cut(s) 296, 412, 481
BstFNI CGCG 2 cut(s) 490, 917
BstKTI GATC 6 cut(s) 262, 361, 369, 462, 503, 538
BstMAI GTCTC 2 cut(s) 5, 869
BstMBI GATC 6 cut(s) 259, 358, 366, 459, 500, 535
BstMCI CGRYCG 2 cut(s) 245, 369
BstMWI GCNNNNNNNGC 1 cut(s) 255
BstNI CCWGG 1 cut(s) 505
BstSCI CCNGG 5 cut(s) 34, 239, 280, 281, 503
BstSFI CTRYAG 1 cut(s) 264
BstSLI GKGCMC 1 cut(s) 709
BstUI CGCG 2 cut(s) 490, 917
BstV1I GCAGC 2 cut(s) 422, 505
BstV2I GAAGAC 2 cut(s) 308, 754
BstX2I RGATCY 3 cut(s) 358, 500, 535
BstYI RGATCY 3 cut(s) 358, 500, 535
BstZI CGGCCG 1 cut(s) 242
BsuRI GGCC 7 cut(s) 244, 280, 427, 616, 707, 721, 856
BtgI CCRYGG 2 cut(s) 617, 874
BtsCI GGATG 3 cut(s) 296, 412, 481
BtsIMutI CAGTG 1 cut(s) 252
Cac8I GCNNGC 3 cut(s) 429, 481, 640
Cfr10I RCCGGY 2 cut(s) 427, 642
Cfr13I GGNCC 7 cut(s) 279, 334, 371, 705, 706, 719, 846
Cfr9I CCCGGG 1 cut(s) 281
CseI GACGC 3 cut(s) 40, 433, 764
Csp6I GTAC 2 cut(s) 676, 913
CviAII CATG 1 cut(s) 583
CviQI GTAC 2 cut(s) 676, 913
DdeI CTNAG 1 cut(s) 178
DpnI GATC 6 cut(s) 261, 360, 368, 461, 502, 537
DpnII GATC 6 cut(s) 259, 358, 366, 459, 500, 535
EaeI YGGCCR 2 cut(s) 242, 425
EagI CGGCCG 1 cut(s) 242
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
EclXI CGGCCG 1 cut(s) 242
Eco130I CCWWGG 3 cut(s) 337, 722, 823
Eco24I GRGCYC 2 cut(s) 515, 709
Eco31I GGTCTC 1 cut(s) 869
Eco47I GGWCC 3 cut(s) 334, 371, 846
Eco52I CGGCCG 1 cut(s) 242
Eco57I CTGAAG 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 281
EcoO109I RGGNCCY 1 cut(s) 706
EcoRII CCWGG 1 cut(s) 503
EcoT14I CCWWGG 3 cut(s) 337, 722, 823
EcoT38I GRGCYC 2 cut(s) 515, 709
ErhI CCWWGG 3 cut(s) 337, 722, 823
Esp3I CGTCTC 1 cut(s) 5
FaeI CATG 1 cut(s) 586
FaiI YATR 8 cut(s) 196, 204, 222, 470, 576, 584, 870, 899
FaqI GGGAC 2 cut(s) 357, 886
FatI CATG 1 cut(s) 582
FauI CCCGC 2 cut(s) 472, 642
Fnu4HI GCNGC 4 cut(s) 411, 491, 494, 609
FokI GGATG 3 cut(s) 303, 419, 488
FriOI GRGCYC 2 cut(s) 515, 709
Fsp4HI GCNGC 4 cut(s) 411, 491, 494, 609
FspBI CTAG 1 cut(s) 791
GluI GCNGC 4 cut(s) 411, 491, 494, 609
HaeIII GGCC 7 cut(s) 244, 280, 427, 616, 707, 721, 856
HapII CCGG 6 cut(s) 35, 241, 277, 282, 428, 643
HgaI GACGC 3 cut(s) 40, 433, 764
Hin1I GRCGYC 2 cut(s) 32, 444
Hin1II CATG 1 cut(s) 586
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HpaII CCGG 6 cut(s) 35, 241, 277, 282, 428, 643
HphI GGTGA 1 cut(s) 92
Hpy166II GTNNAC 1 cut(s) 7
Hpy188III TCNNGA 3 cut(s) 364, 533, 602
Hpy8I GTNNAC 1 cut(s) 7
Hpy99I CGWCG 1 cut(s) 597
HpyAV CCTTC 2 cut(s) 109, 204
HpyCH4III ACNGT 6 cut(s) 16, 150, 160, 597, 743, 875
HpyCH4V TGCA 5 cut(s) 101, 266, 410, 574, 665
HpyF10VI GCNNNNNNNGC 1 cut(s) 255
HpyF3I CTNAG 1 cut(s) 178
Hsp92I GRCGYC 2 cut(s) 32, 444
Hsp92II CATG 1 cut(s) 586
KroI GCCGGC 1 cut(s) 427
KroNI GCCGGC 1 cut(s) 429
Kzo9I GATC 6 cut(s) 259, 358, 366, 459, 500, 535
LmnI GCTCC 2 cut(s) 589, 726
Lsp1109I GCAGC 2 cut(s) 422, 505
LweI GCATC 3 cut(s) 319, 397, 466
MaeI CTAG 1 cut(s) 791
MaeIII GTNAC 3 cut(s) 28, 144, 160
MalI GATC 6 cut(s) 261, 360, 368, 461, 502, 537
MboI GATC 6 cut(s) 259, 358, 366, 459, 500, 535
MboII GAAGA 4 cut(s) 131, 308, 754, 761
MfeI CAATTG 1 cut(s) 552
MflI RGATCY 3 cut(s) 358, 500, 535
MhlI GDGCHC 4 cut(s) 251, 515, 709, 731
MluCI AATT 3 cut(s) 552, 564, 795
MluI ACGCGT 1 cut(s) 915
MroNI GCCGGC 1 cut(s) 427
MspA1I CMGCKG 1 cut(s) 396
MspI CCGG 6 cut(s) 35, 241, 277, 282, 428, 643
MspR9I CCNGG 5 cut(s) 36, 241, 282, 283, 505
MunI CAATTG 1 cut(s) 552
MvaI CCWGG 1 cut(s) 505
MvnI CGCG 2 cut(s) 490, 917
MwoI GCNNNNNNNGC 1 cut(s) 255
NaeI GCCGGC 1 cut(s) 429
NciI CCSGG 4 cut(s) 36, 241, 282, 283
NdeII GATC 6 cut(s) 259, 358, 366, 459, 500, 535
NgoMIV GCCGGC 1 cut(s) 427
NlaIII CATG 1 cut(s) 586
NlaIV GGNNCC 5 cut(s) 373, 502, 707, 708, 848
NmeAIII GCCGAG 1 cut(s) 270
NmuCI GTSAC 2 cut(s) 28, 160
PdiI GCCGGC 1 cut(s) 429
PflFI GACNNNGTC 1 cut(s) 758
PkrI GCNGC 4 cut(s) 412, 492, 495, 610
Ple19I CGATCG 1 cut(s) 369
Psp6I CCWGG 1 cut(s) 503
PspGI CCWGG 1 cut(s) 503
PspN4I GGNNCC 5 cut(s) 373, 502, 707, 708, 848
PspOMI GGGCCC 1 cut(s) 705
PspPI GGNCC 7 cut(s) 279, 334, 371, 705, 706, 719, 846
PstI CTGCAG 1 cut(s) 268
PsuI RGATCY 3 cut(s) 358, 500, 535
PsyI GACNNNGTC 1 cut(s) 758
PvuI CGATCG 1 cut(s) 369
RsaI GTAC 2 cut(s) 677, 914
RsaNI GTAC 2 cut(s) 676, 913
SatI GCNGC 4 cut(s) 411, 491, 494, 609
Sau3AI GATC 6 cut(s) 259, 358, 366, 459, 500, 535
Sau96I GGNCC 7 cut(s) 279, 334, 371, 705, 706, 719, 846
ScaI AGTACT 1 cut(s) 677
ScrFI CCNGG 5 cut(s) 36, 241, 282, 283, 505
SduI GDGCHC 4 cut(s) 251, 515, 709, 731
SetI ASST 7 cut(s) 28, 82, 89, 174, 774, 786, 896
SfaNI GCATC 3 cut(s) 319, 397, 466
SfcI CTRYAG 1 cut(s) 264
SinI GGWCC 3 cut(s) 334, 371, 846
SmaI CCCGGG 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 682
SmoI CTYRAG 1 cut(s) 682
Sse9I AATT 3 cut(s) 552, 564, 795
SsiI CCGC 8 cut(s) 332, 396, 431, 479, 490, 545, 608, 649
SspMI CTAG 1 cut(s) 791
StyD4I CCNGG 5 cut(s) 34, 239, 280, 281, 503
StyI CCWWGG 3 cut(s) 337, 722, 823
TaaI ACNGT 6 cut(s) 16, 150, 160, 597, 743, 875
TaqI TCGA 1 cut(s) 365
TaqII GACCGA 1 cut(s) 359
TasI AATT 3 cut(s) 552, 564, 795
TatI WGTACW 1 cut(s) 675
TauI GCSGC 2 cut(s) 493, 611
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 2 cut(s) 28, 160
TseI GCWGC 2 cut(s) 410, 493
Tsp45I GTSAC 2 cut(s) 28, 160
TspMI CCCGGG 1 cut(s) 281
TspRI CASTG 1 cut(s) 259
Tth111I GACNNNGTC 1 cut(s) 758
VpaK11BI GGWCC 3 cut(s) 334, 371, 846
XapI RAATTY 2 cut(s) 564, 795
XmaI CCCGGG 1 cut(s) 281
XspI CTAG 1 cut(s) 791
ZrmI AGTACT 1 cut(s) 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.