Rmu_sc0003352.1_g000003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003352.1
OG0016293
Physical Location & Seq
Reverse (-)
10454 .. 10963
510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003352.1_g000003.1.cds

Sequence Viewer

Length: 510 bp
atgtggtatcttagtagaacaccttcaaaccgcaaaccaaatgatgactccagcttcagtttgcgcaattccatcttcaccgtctccgattccggttttttccaagacatttgcatgattgaggtgccaaacaaatccgtggttgatcacgaagaacacaacaaagaagaagagacgtgcccattttcattctcattcccaaaatgggaatggtttgcctatctctgctctcccacccaattccattccgtggcggtgcctcgaaactcttccatgtttagagtacaggtgattgggattctgcctcaacatttcttgtgtgccaagttggtgcgaggtccgaaaccttatgtcactctgtgcacttactcatgcagtgagaattctctcaatgatgtgcccaaaatgaactgtgcggatcgcaatctgttcggagctgcatgtgctattcttgatgggtggctatatgttgctggagggtataaggataactctttaggtaagtattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1

Protein Analysis

169

Amino Acids

19.23

Weight (kDa)

6.4

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 65
AccB1I GGYRCC 2 cut(s) 124, 256
AccB7I CCANNNNNTGG 1 cut(s) 250
AciI CCGC 3 cut(s) 31, 254, 416
AclWI GGATC 1 cut(s) 426
AcsI RAATTY 1 cut(s) 382
AcuI CTGAAG 1 cut(s) 40
AdeI CACNNNGTG 1 cut(s) 360
AfaI GTAC 1 cut(s) 285
AfiI CCNNNNNNNGG 2 cut(s) 205, 250
AgsI TTSAA 1 cut(s) 27
AjiI CACGTC 1 cut(s) 177
AluBI AGCT 2 cut(s) 54, 437
AluI AGCT 2 cut(s) 54, 437
Alw21I GWGCWC 1 cut(s) 365
Alw26I GTCTC 2 cut(s) 88, 167
Alw44I GTGCAC 1 cut(s) 361
AlwI GGATC 1 cut(s) 426
ApaLI GTGCAC 1 cut(s) 361
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 1 cut(s) 382
Asp700I GAANNNNTTC 2 cut(s) 22, 268
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 1 cut(s) 338
AsuHPI GGTGA 2 cut(s) 70, 301
AvaII GGWCC 1 cut(s) 338
BaeGI GKGCMC 3 cut(s) 182, 365, 402
BanI GGYRCC 2 cut(s) 124, 256
BarI GAAGNNNNNNTAC 2 cut(s) 7, 39
Bbv12I GWGCWC 1 cut(s) 365
BbvI GCAGC 1 cut(s) 424
BccI CCATC 2 cut(s) 80, 449
BcgI CGANNNNNNTGC 2 cut(s) 412, 446
BclI TGATCA 1 cut(s) 145
BcoDI GTCTC 2 cut(s) 88, 167
BisI GCNGC 1 cut(s) 438
BlsI GCNGC 1 cut(s) 439
Bme18I GGWCC 1 cut(s) 338
BmgBI CACGTC 1 cut(s) 177
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 2 cut(s) 126, 258
BpmI CTGGAG 2 cut(s) 34, 495
BsaBI GATNNNNATC 1 cut(s) 423
BsaJI CCNNGG 2 cut(s) 138, 249
BsaWI WCCGGW 1 cut(s) 92
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 250
Bse8I GATNNNNATC 1 cut(s) 423
BseDI CCNNGG 2 cut(s) 138, 249
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 2 cut(s) 205, 250
BseSI GKGCMC 3 cut(s) 182, 365, 402
BseXI GCAGC 1 cut(s) 424
BshNI GGYRCC 2 cut(s) 124, 256
BsiHKAI GWGCWC 1 cut(s) 365
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 2 cut(s) 205, 250
BsmAI GTCTC 2 cut(s) 88, 167
BsmBI CGTCTC 2 cut(s) 88, 167
Bsp1286I GDGCHC 3 cut(s) 182, 365, 402
Bsp143I GATC 2 cut(s) 145, 418
BspACI CCGC 3 cut(s) 31, 254, 416
BspLI GGNNCC 2 cut(s) 126, 258
BspPI GGATC 1 cut(s) 426
BspT107I GGYRCC 2 cut(s) 124, 256
BssECI CCNNGG 2 cut(s) 138, 249
BssMI GATC 2 cut(s) 145, 418
Bst4CI ACNGT 2 cut(s) 82, 413
Bst6I CTCTTC 2 cut(s) 165, 274
BstDEI CTNAG 1 cut(s) 11
BstDSI CCRYGG 2 cut(s) 138, 249
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 2 cut(s) 148, 421
BstMAI GTCTC 2 cut(s) 88, 167
BstMBI GATC 2 cut(s) 145, 418
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstNSI RCATGY 1 cut(s) 444
BstSLI GKGCMC 3 cut(s) 182, 365, 402
BstV1I GCAGC 1 cut(s) 424
BtgI CCRYGG 2 cut(s) 138, 249
BtrI CACGTC 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 382
BtsIMutI CAGTG 1 cut(s) 382
CfoI GCGC 1 cut(s) 66
Cfr13I GGNCC 1 cut(s) 338
Csp6I GTAC 1 cut(s) 284
CviAII CATG 4 cut(s) 115, 274, 372, 441
CviJI RGCY 3 cut(s) 54, 437, 463
CviKI_1 RGCY 3 cut(s) 54, 437, 463
CviQI GTAC 1 cut(s) 284
DdeI CTNAG 1 cut(s) 11
DpnI GATC 2 cut(s) 147, 420
DpnII GATC 2 cut(s) 145, 418
DraIII CACNNNGTG 1 cut(s) 360
Eam1104I CTCTTC 2 cut(s) 165, 274
EarI CTCTTC 2 cut(s) 165, 274
Eco47I GGWCC 1 cut(s) 338
Eco57I CTGAAG 1 cut(s) 40
EcoRI GAATTC 1 cut(s) 382
Esp3I CGTCTC 2 cut(s) 88, 167
FaeI CATG 4 cut(s) 118, 277, 375, 444
FaiI YATR 8 cut(s) 116, 275, 351, 373, 442, 466, 468, 483
FatI CATG 4 cut(s) 114, 273, 371, 440
FbaI TGATCA 1 cut(s) 145
Fnu4HI GCNGC 1 cut(s) 438
Fsp4HI GCNGC 1 cut(s) 438
FspI TGCGCA 1 cut(s) 65
GlaI GCGC 1 cut(s) 65
GluI GCNGC 1 cut(s) 438
GsuI CTGGAG 2 cut(s) 34, 495
HapII CCGG 1 cut(s) 93
HhaI GCGC 1 cut(s) 66
Hin1II CATG 4 cut(s) 118, 277, 375, 444
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HinfI GANTC 3 cut(s) 47, 89, 298
HpaII CCGG 1 cut(s) 93
HphI GGTGA 2 cut(s) 70, 301
Hpy166II GTNNAC 1 cut(s) 363
Hpy188I TCNGA 3 cut(s) 88, 342, 434
Hpy188III TCNNGA 2 cut(s) 149, 452
Hpy8I GTNNAC 1 cut(s) 363
HpyAV CCTTC 1 cut(s) 33
HpyCH4III ACNGT 2 cut(s) 82, 413
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 4 cut(s) 114, 363, 375, 440
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 1 cut(s) 11
HpySE526I ACGT 1 cut(s) 176
Hsp92II CATG 4 cut(s) 118, 277, 375, 444
HspAI GCGC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 2 cut(s) 145, 418
LmnI GCTCC 1 cut(s) 434
LpnPI CCDG 4 cut(s) 64, 106, 272, 459
Lsp1109I GCAGC 1 cut(s) 424
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 147, 420
MboI GATC 2 cut(s) 145, 418
MboII GAAGA 5 cut(s) 67, 164, 179, 182, 261
MhlI GDGCHC 3 cut(s) 182, 365, 402
MluCI AATT 3 cut(s) 67, 239, 382
MlyI GAGTC 1 cut(s) 41
MnlI CCTC 5 cut(s) 115, 270, 315, 329, 470
MroXI GAANNNNTTC 2 cut(s) 22, 268
MslI CAYNNNNRTG 1 cut(s) 113
MspI CCGG 1 cut(s) 93
MwoI GCNNNNNNNGC 1 cut(s) 443
NdeII GATC 2 cut(s) 145, 418
NlaIII CATG 4 cut(s) 118, 277, 375, 444
NlaIV GGNNCC 2 cut(s) 126, 258
NmuCI GTSAC 1 cut(s) 352
NsbI TGCGCA 1 cut(s) 65
NspI RCATGY 1 cut(s) 444
PdmI GAANNNNTTC 2 cut(s) 22, 268
PfeI GAWTC 2 cut(s) 89, 298
PflMI CCANNNNNTGG 1 cut(s) 250
PkrI GCNGC 1 cut(s) 439
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
PspN4I GGNNCC 2 cut(s) 126, 258
PspPI GGNCC 1 cut(s) 338
RsaI GTAC 1 cut(s) 285
RsaNI GTAC 1 cut(s) 284
RseI CAYNNNNRTG 1 cut(s) 113
SatI GCNGC 1 cut(s) 438
Sau3AI GATC 2 cut(s) 145, 418
Sau96I GGNCC 1 cut(s) 338
SchI GAGTC 1 cut(s) 41
SduI GDGCHC 3 cut(s) 182, 365, 402
SetI ASST 9 cut(s) 25, 56, 126, 179, 291, 340, 349, 439, 502
SinI GGWCC 1 cut(s) 338
SmiMI CAYNNNNRTG 1 cut(s) 113
Sse9I AATT 3 cut(s) 67, 239, 382
SsiI CCGC 3 cut(s) 31, 254, 416
TaaI ACNGT 2 cut(s) 82, 413
TaiI ACGT 1 cut(s) 179
TaqI TCGA 1 cut(s) 262
TasI AATT 3 cut(s) 67, 239, 382
TatI WGTACW 1 cut(s) 283
TfiI GAWTC 2 cut(s) 89, 298
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 2 cut(s) 177, 422
TspGWI ACGGA 2 cut(s) 127, 238
TspRI CASTG 1 cut(s) 382
Van91I CCANNNNNTGG 1 cut(s) 250
VneI GTGCAC 1 cut(s) 361
VpaK11BI GGWCC 1 cut(s) 338
XapI RAATTY 1 cut(s) 382
XceI RCATGY 1 cut(s) 444
XcmI CCANNNNNNNNNTGG 1 cut(s) 207
XmnI GAANNNNTTC 2 cut(s) 22, 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.