Rmu_sc0003459.1_g000025

Translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003459.1
OG0002846
Physical Location & Seq
Reverse (-)
138056 .. 141006
2951 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1965 bp
atggtggaaaattttttgtatagatgctatcatgggagaaatgggaatgaccagtcatttggtttaaaacctcccaagagagagaagtttgtaaagagggacaataaggcacagcctcctgttgatgctccatatgtgcctcctaaaccacaaagaactaccaaggccatgccagataagacaattgagatttttgaaggtatgacaattgatgaacttgccaagcgcactggacagtcaatatctactctgcaaaccattcttacaaatgttggggaaaaggttggctcggagtttgatactctcagcgtcgacattgcagagctggttgcaatggaagttggggtcaatgtgaggaggctgcactccaatgaaggtacagaaattctaccacgacctccagttgtaactgtcatgggccatgttgaccatgggaaaacttcacttttggatgctctacgtcagacgtctgtggctgccaaagaagctggagggattactcagcatgttggtgcttttgttgtgggcatgacatctggagcgtcaattacatttcttgacacccctggtcatgcagcatttagtgcgatgcgagctagaggggctgccgttacagatatagttgtgcttgttgtagctgctgatgatggggtgatgcctcagactcttgaagccatggcccatgcaaaggcagctaaagtaccaattgttgttgcaattaataaatgtgataaaccagctgctaacgcagaaaaggtaagactccagcttgcgtcggagggcttgctgctagaggatatgggtggggatgttcaggttgttgaggtttcagctatgaagaagactggtttagataacttggaggaggctttgcttctccaggctgagatgatggatctgaaagctcgtattgatggaccggcccaagcttatgtggtggaagcaagacttgataggggaaagggtccattggttactgcaatagtgaaagcaggaactttaatttgtgggaaatatgttgtcgtgggctccgagtggggtagaataagggctattcgggacatggcagggaagtcgacagagcgggcaacacctgcgatgccagttgagattgaaggcctgaagggacttccaagggctggagatgatatcattgttgtggaatccgaggagcgagctagaatgcttagtgcagggaggaaaaggaagttcgagaaagatagactcatgaaaattatggatggaagggtagaagatccagaaacagaaccatcagatgaggcgcccaagcgggttgaattgccgataatagtaaaaggggatgtgcagggtactgtccaagctgttacagatgcattagggagtttaaatagtcctcaggtttttgtgaatgtggtacatgttggtgttgggtcactatctcagtctgatgtggacatggcacaagcctgtggtgcatgtattattggatttaacatcaaggctccacccagttccatcagtttggctgcgtctcgagccaatatcaagataatgcaacaccgtgtgatctatcatcttctggaagacattgggaactttatagttaagaaggcccctgggactaccgagaccaaggttgctggggaagccgaggttcttagtatttttgagctcaaaggaaggagcaagtctaagggacctgatgtgaagattgccggatgccgggtggttgatggttttgtcactaaatcggcaaccctaaggcttcttaggagtggagaagttatatttgagggatcatgtgaatcgcttaagcgggagaaacaggatgtggacacggtaaagaaggggaatgagtgtggactggtgatccaaaattgctatgatttccaggttggtgacatggtccagtgcttgcagcaagttataaggaagcccaaattcatttcatcggagagtggtgctgttcgaatagagtgttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP_EFTU IF-2

Protein Analysis

654

Amino Acids

70.39

Weight (kDa)

7.13

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 136 - 292 3.1e-33 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 136 - 242 1.3e-09 50S ribosome-binding GTPase
FeoB_N PF02421 136 - 290 4e-06 Ferrous iron transport protein B
EF-G_D2 PF22042 307 - 386 3.5e-19 Elongation factor G domain 2
IF-2 PF11987 430 - 524 3.4e-28 Translation-initiation factor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1910
AarI CACCTGC 1 cut(s) 1102
AatII GACGTC 1 cut(s) 470
Acc36I ACCTGC 1 cut(s) 1102
AccB1I GGYRCC 1 cut(s) 1282
AccB7I CCANNNNNTGG 1 cut(s) 1139
AccBSI CCGCTC 1 cut(s) 1084
AccI GTMKAC 2 cut(s) 312, 1076
AciI CCGC 3 cut(s) 1084, 1291, 1798
AclWI GGATC 4 cut(s) 903, 1250, 1786, 1846
AcsI RAATTY 3 cut(s) 10, 384, 1922
AcuI CTGAAG 1 cut(s) 1142
AcyI GRCGYC 2 cut(s) 467, 1283
AdeI CACNNNGTG 1 cut(s) 1546
AfaI GTAC 4 cut(s) 379, 702, 1333, 1398
AfiI CCNNNNNNNGG 3 cut(s) 688, 1139, 1704
AflII CTTAAG 1 cut(s) 1793
AflIII ACRYGT 1 cut(s) 1399
AgsI TTSAA 4 cut(s) 197, 671, 1115, 1298
AjnI CCWGG 4 cut(s) 565, 879, 1597, 1872
Alw21I GWGCWC 1 cut(s) 1656
Alw26I GTCTC 2 cut(s) 1518, 1604
AlwI GGATC 4 cut(s) 903, 1250, 1786, 1846
Ama87I CYCGRG 1 cut(s) 1515
AoxI GGCC 6 cut(s) 165, 418, 678, 921, 1117, 1593
ApoI RAATTY 3 cut(s) 10, 384, 1922
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
AseI ATTAAT 1 cut(s) 720
AspLEI GCGC 2 cut(s) 228, 1285
AspS9I GGNCC 8 cut(s) 418, 679, 917, 922, 965, 1594, 1679, 1888
AsuC2I CCSGG 1 cut(s) 1706
AsuHPI GGTGA 3 cut(s) 664, 1861, 1892
AsuII TTCGAA 1 cut(s) 1951
AvaI CYCGRG 1 cut(s) 1515
AvaII GGWCC 4 cut(s) 917, 965, 1679, 1888
AxyI CCTNAGG 2 cut(s) 1377, 1742
BanI GGYRCC 1 cut(s) 1282
BanII GRGCYC 2 cut(s) 1031, 1656
BbsI GAAGAC 2 cut(s) 848, 1572
Bbv12I GWGCWC 1 cut(s) 1656
BccI CCATC 7 cut(s) 641, 886, 908, 1235, 1279, 1505, 1709
BceAI ACGGC 1 cut(s) 593
BcgI CGANNNNNNTGC 2 cut(s) 1056, 1090
BciT130I CCWGG 4 cut(s) 567, 881, 1599, 1874
BcnI CCSGG 1 cut(s) 1706
BcoDI GTCTC 2 cut(s) 1518, 1604
BfaI CTAG 3 cut(s) 597, 791, 1179
BfoI RGCGCY 1 cut(s) 1286
BfrI CTTAAG 1 cut(s) 1793
BfuAI ACCTGC 1 cut(s) 1102
Bme1390I CCNGG 5 cut(s) 567, 881, 1599, 1706, 1874
Bme18I GGWCC 4 cut(s) 917, 965, 1679, 1888
BmeT110I CYCGRG 1 cut(s) 1515
BmgT120I GGNCC 8 cut(s) 418, 679, 917, 922, 965, 1594, 1679, 1888
BmiI GGNNCC 6 cut(s) 966, 1030, 1284, 1485, 1596, 1680
BmrFI CCNGG 5 cut(s) 567, 881, 1599, 1706, 1874
BmrI ACTGGG 1 cut(s) 1485
BmsI GCATC 8 cut(s) 14, 115, 442, 579, 645, 1089, 1342, 1691
BmuI ACTGGG 1 cut(s) 1485
BpiI GAAGAC 2 cut(s) 848, 1572
BpmI CTGGAG 6 cut(s) 384, 510, 558, 749, 863, 1161
Bpu14I TTCGAA 1 cut(s) 1951
BpuMI CCSGG 1 cut(s) 1706
BsaHI GRCGYC 2 cut(s) 467, 1283
BsaI GGTCTC 1 cut(s) 1604
Bsc4I CCNNNNNNNGG 3 cut(s) 688, 1139, 1704
Bse118I RCCGGY 1 cut(s) 919
Bse1I ACTGG 8 cut(s) 52, 235, 401, 850, 1103, 1491, 1851, 1891
Bse21I CCTNAGG 2 cut(s) 1377, 1742
Bse3DI GCAATG 2 cut(s) 315, 339
BseBI CCWGG 4 cut(s) 567, 881, 1599, 1874
BseGI GGATG 6 cut(s) 457, 814, 1246, 1327, 1706, 1816
BseLI CCNNNNNNNGG 3 cut(s) 688, 1139, 1704
BseMI GCAATG 2 cut(s) 315, 339
BseMII CTCAG 6 cut(s) 319, 515, 674, 876, 1391, 1436
BseNI ACTGG 8 cut(s) 52, 235, 401, 850, 1103, 1491, 1851, 1891
BseRI GAGGAG 3 cut(s) 370, 878, 1184
BseYI CCCAGC 1 cut(s) 1622
BsgI GTGCAG 3 cut(s) 347, 1212, 1346
BshFI GGCC 6 cut(s) 167, 420, 680, 923, 1119, 1595
BshNI GGYRCC 1 cut(s) 1282
BsiHKAI GWGCWC 1 cut(s) 1656
BsiHKCI CYCGRG 1 cut(s) 1515
BsiSI CCGG 3 cut(s) 920, 1698, 1705
BslFI GGGAC 5 cut(s) 113, 1073, 1140, 1615, 1692
BslI CCNNNNNNNGG 3 cut(s) 688, 1139, 1704
BsmAI GTCTC 2 cut(s) 1518, 1604
BsmBI CGTCTC 1 cut(s) 1518
BsmFI GGGAC 5 cut(s) 113, 1073, 1140, 1615, 1692
BsmI GAATGC 1 cut(s) 1188
BsnI GGCC 6 cut(s) 167, 420, 680, 923, 1119, 1595
Bso31I GGTCTC 1 cut(s) 1604
BsoBI CYCGRG 1 cut(s) 1515
Bsp119I TTCGAA 1 cut(s) 1951
Bsp1286I GDGCHC 2 cut(s) 1031, 1656
Bsp143I GATC 5 cut(s) 895, 1255, 1548, 1778, 1851
Bsp19I CCATGG 2 cut(s) 430, 675
BspACI CCGC 3 cut(s) 1084, 1291, 1798
BspANI GGCC 6 cut(s) 167, 420, 680, 923, 1119, 1595
BspCNI CTCAG 6 cut(s) 318, 514, 673, 877, 1390, 1435
BspHI TCATGA 1 cut(s) 1227
BspLI GGNNCC 6 cut(s) 966, 1030, 1284, 1485, 1596, 1680
BspMI ACCTGC 1 cut(s) 1102
BspPI GGATC 4 cut(s) 903, 1250, 1786, 1846
BspT104I TTCGAA 1 cut(s) 1951
BspT107I GGYRCC 1 cut(s) 1282
BspTI CTTAAG 1 cut(s) 1793
BspTNI GGTCTC 1 cut(s) 1604
BsrBI CCGCTC 1 cut(s) 1084
BsrDI GCAATG 2 cut(s) 315, 339
BsrFI RCCGGY 1 cut(s) 919
BsrI ACTGG 8 cut(s) 52, 235, 401, 850, 1103, 1491, 1851, 1891
BssAI RCCGGY 1 cut(s) 919
BssMI GATC 5 cut(s) 895, 1255, 1548, 1778, 1851
BssNI GRCGYC 2 cut(s) 467, 1283
BssT1I CCWWGG 5 cut(s) 162, 430, 675, 1133, 1614
Bst2UI CCWGG 4 cut(s) 567, 881, 1599, 1874
Bst4CI ACNGT 5 cut(s) 237, 412, 1336, 1544, 1822
BstACI GRCGYC 2 cut(s) 467, 1283
BstAFI CTTAAG 1 cut(s) 1793
BstAPI GCANNNNNTGC 2 cut(s) 584, 1094
BstBI TTCGAA 1 cut(s) 1951
BstC8I GCNNGC 6 cut(s) 594, 771, 785, 1086, 1176, 1898
BstDSI CCRYGG 2 cut(s) 430, 675
BstF5I GGATG 6 cut(s) 457, 814, 1246, 1327, 1706, 1816
BstH2I RGCGCY 1 cut(s) 1286
BstHHI GCGC 2 cut(s) 228, 1285
BstKTI GATC 5 cut(s) 898, 1258, 1551, 1781, 1854
BstMAI GTCTC 2 cut(s) 1518, 1604
BstMBI GATC 5 cut(s) 895, 1255, 1548, 1778, 1851
BstNI CCWGG 4 cut(s) 567, 881, 1599, 1874
BstNSI RCATGY 3 cut(s) 509, 1403, 1461
BstSCI CCNGG 5 cut(s) 565, 879, 1597, 1704, 1872
BstV2I GAAGAC 2 cut(s) 848, 1572
BstX2I RGATCY 2 cut(s) 895, 1255
BstXI CCANNNNNNTGG 2 cut(s) 59, 1504
BstYI RGATCY 2 cut(s) 895, 1255
Bsu36I CCTNAGG 2 cut(s) 1377, 1742
BsuRI GGCC 6 cut(s) 167, 420, 680, 923, 1119, 1595
BtgI CCRYGG 2 cut(s) 430, 675
BtgZI GCGATG 2 cut(s) 602, 1112
BtsCI GGATG 6 cut(s) 457, 814, 1246, 1327, 1706, 1816
BtsIMutI CAGTG 2 cut(s) 228, 1898
BveI ACCTGC 1 cut(s) 1102
Cac8I GCNNGC 6 cut(s) 594, 771, 785, 1086, 1176, 1898
CciI TCATGA 1 cut(s) 1227
CfoI GCGC 2 cut(s) 228, 1285
Cfr10I RCCGGY 1 cut(s) 919
Cfr13I GGNCC 8 cut(s) 418, 679, 917, 922, 965, 1594, 1679, 1888
CseI GACGC 4 cut(s) 298, 531, 762, 1500
Csp6I GTAC 4 cut(s) 378, 701, 1332, 1397
CviQI GTAC 4 cut(s) 378, 701, 1332, 1397
DinI GGCGCC 1 cut(s) 1284
DpnI GATC 5 cut(s) 897, 1257, 1550, 1780, 1853
DpnII GATC 5 cut(s) 895, 1255, 1548, 1778, 1851
DraI TTTAAA 2 cut(s) 66, 1368
DraIII CACNNNGTG 1 cut(s) 1546
Ecl136II GAGCTC 1 cut(s) 1654
Eco130I CCWWGG 5 cut(s) 162, 430, 675, 1133, 1614
Eco147I AGGCCT 1 cut(s) 1119
Eco24I GRGCYC 2 cut(s) 1031, 1656
Eco31I GGTCTC 1 cut(s) 1604
Eco32I GATATC 1 cut(s) 1150
Eco47I GGWCC 4 cut(s) 917, 965, 1679, 1888
Eco53kI GAGCTC 1 cut(s) 1654
Eco57I CTGAAG 1 cut(s) 1142
Eco81I CCTNAGG 2 cut(s) 1377, 1742
Eco88I CYCGRG 1 cut(s) 1515
EcoICRI GAGCTC 1 cut(s) 1654
EcoO109I RGGNCCY 2 cut(s) 1594, 1679
EcoRII CCWGG 4 cut(s) 565, 879, 1597, 1872
EcoRV GATATC 1 cut(s) 1150
EcoT14I CCWWGG 5 cut(s) 162, 430, 675, 1133, 1614
EcoT22I ATGCAT 1 cut(s) 1357
EcoT38I GRGCYC 2 cut(s) 1031, 1656
EgeI GGCGCC 1 cut(s) 1284
EheI GGCGCC 1 cut(s) 1284
ErhI CCWWGG 5 cut(s) 162, 430, 675, 1133, 1614
Esp3I CGTCTC 1 cut(s) 1518
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 5 cut(s) 113, 1073, 1140, 1615, 1692
FauI CCCGC 3 cut(s) 1077, 1284, 1791
FauNDI CATATG 1 cut(s) 133
FblI GTMKAC 2 cut(s) 312, 1076
FokI GGATG 6 cut(s) 464, 821, 1253, 1334, 1713, 1823
FriOI GRGCYC 2 cut(s) 1031, 1656
FspBI CTAG 3 cut(s) 597, 791, 1179
GlaI GCGC 2 cut(s) 227, 1284
GsaI CCCAGC 1 cut(s) 1626
GsuI CTGGAG 6 cut(s) 384, 510, 558, 749, 863, 1161
HaeII RGCGCY 1 cut(s) 1286
HaeIII GGCC 6 cut(s) 167, 420, 680, 923, 1119, 1595
HapII CCGG 3 cut(s) 920, 1698, 1705
HgaI GACGC 4 cut(s) 298, 531, 762, 1500
HhaI GCGC 2 cut(s) 228, 1285
Hin1I GRCGYC 2 cut(s) 467, 1283
Hin6I GCGC 2 cut(s) 226, 1283
HinP1I GCGC 2 cut(s) 226, 1283
HincII GTYRAC 3 cut(s) 313, 427, 1077
HindII GTYRAC 3 cut(s) 313, 427, 1077
HindIII AAGCTT 1 cut(s) 927
HinfI GANTC 5 cut(s) 664, 762, 1163, 1224, 1787
HpaII CCGG 3 cut(s) 920, 1698, 1705
HphI GGTGA 3 cut(s) 664, 1861, 1892
Hpy166II GTNNAC 6 cut(s) 313, 427, 1077, 1435, 1816, 1844
Hpy8I GTNNAC 6 cut(s) 313, 427, 1077, 1435, 1816, 1844
Hpy99I CGWCG 2 cut(s) 314, 778
HpyAV CCTTC 8 cut(s) 191, 368, 1109, 1117, 1239, 1585, 1656, 1822
HpyCH4III ACNGT 5 cut(s) 237, 412, 1336, 1544, 1822
HpyCH4IV ACGT 2 cut(s) 460, 467
HpySE526I ACGT 2 cut(s) 460, 467
Hsp92I GRCGYC 2 cut(s) 467, 1283
HspAI GCGC 2 cut(s) 226, 1283
KasI GGCGCC 1 cut(s) 1282
Kzo9I GATC 5 cut(s) 895, 1255, 1548, 1778, 1851
LmnI GCTCC 6 cut(s) 133, 539, 1034, 1171, 1489, 1665
LweI GCATC 8 cut(s) 14, 115, 442, 579, 645, 1089, 1342, 1691
MaeI CTAG 3 cut(s) 597, 791, 1179
MaeII ACGT 2 cut(s) 460, 467
MaeIII GTNAC 7 cut(s) 406, 610, 973, 1345, 1413, 1723, 1880
MalI GATC 5 cut(s) 897, 1257, 1550, 1780, 1853
MbiI CCGCTC 1 cut(s) 1084
MboI GATC 5 cut(s) 895, 1255, 1548, 1778, 1851
MboII GAAGA 6 cut(s) 850, 853, 1265, 1550, 1577, 1702
MfeI CAATTG 3 cut(s) 183, 207, 705
MflI RGATCY 2 cut(s) 895, 1255
MhlI GDGCHC 2 cut(s) 1031, 1656
Mly113I GGCGCC 1 cut(s) 1283
MlyI GAGTC 3 cut(s) 658, 756, 1218
MmeI TCCRAC 1 cut(s) 756
Mph1103I ATGCAT 1 cut(s) 1357
MseI TTAA 7 cut(s) 65, 720, 1001, 1367, 1473, 1587, 1794
MslI CAYNNNNRTG 4 cut(s) 369, 510, 1157, 1404
MspA1I CMGCKG 1 cut(s) 740
MspCI CTTAAG 1 cut(s) 1793
MspI CCGG 3 cut(s) 920, 1698, 1705
MspR9I CCNGG 5 cut(s) 567, 881, 1599, 1706, 1874
MunI CAATTG 3 cut(s) 183, 207, 705
Mva1269I GAATGC 1 cut(s) 1188
MvaI CCWGG 4 cut(s) 567, 881, 1599, 1874
NarI GGCGCC 1 cut(s) 1283
NciI CCSGG 1 cut(s) 1706
NcoI CCATGG 2 cut(s) 430, 675
NdeI CATATG 1 cut(s) 133
NdeII GATC 5 cut(s) 895, 1255, 1548, 1778, 1851
NlaIV GGNNCC 6 cut(s) 966, 1030, 1284, 1485, 1596, 1680
NmeAIII GCCGAG 1 cut(s) 1657
NmuCI GTSAC 3 cut(s) 1413, 1723, 1880
NsiI ATGCAT 1 cut(s) 1357
NspI RCATGY 3 cut(s) 509, 1403, 1461
NspV TTCGAA 1 cut(s) 1951
PaeR7I CTCGAG 1 cut(s) 1515
PagI TCATGA 1 cut(s) 1227
PaqCI CACCTGC 1 cut(s) 1102
PasI CCCWGGG 1 cut(s) 1598
PceI AGGCCT 1 cut(s) 1119
PciI ACATGT 1 cut(s) 1399
PcsI WCGNNNNNNNCGW 1 cut(s) 1029
PctI GAATGC 1 cut(s) 1188
PfeI GAWTC 2 cut(s) 1163, 1787
PflFI GACNNNGTC 1 cut(s) 1886
PflMI CCANNNNNTGG 1 cut(s) 1139
PleI GAGTC 3 cut(s) 658, 756, 1218
PluTI GGCGCC 1 cut(s) 1286
PpsI GAGTC 3 cut(s) 658, 756, 1218
PpuMI RGGWCCY 1 cut(s) 1679
PscI ACATGT 1 cut(s) 1399
PshBI ATTAAT 1 cut(s) 720
PsiI TTATAA 1 cut(s) 1910
Psp124BI GAGCTC 1 cut(s) 1656
Psp5II RGGWCCY 1 cut(s) 1679
Psp6I CCWGG 4 cut(s) 565, 879, 1597, 1872
PspFI CCCAGC 1 cut(s) 1622
PspGI CCWGG 4 cut(s) 565, 879, 1597, 1872
PspN4I GGNNCC 6 cut(s) 966, 1030, 1284, 1485, 1596, 1680
PspPI GGNCC 8 cut(s) 418, 679, 917, 922, 965, 1594, 1679, 1888
PspPPI RGGWCCY 1 cut(s) 1679
PsuI RGATCY 2 cut(s) 895, 1255
PsyI GACNNNGTC 1 cut(s) 1886
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 4 cut(s) 379, 702, 1333, 1398
RsaNI GTAC 4 cut(s) 378, 701, 1332, 1397
RseI CAYNNNNRTG 4 cut(s) 369, 510, 1157, 1404
SacI GAGCTC 1 cut(s) 1656
SalI GTCGAC 2 cut(s) 311, 1075
SaqAI TTAA 7 cut(s) 65, 720, 1001, 1367, 1473, 1587, 1794
Sau3AI GATC 5 cut(s) 895, 1255, 1548, 1778, 1851
Sau96I GGNCC 8 cut(s) 418, 679, 917, 922, 965, 1594, 1679, 1888
SchI GAGTC 3 cut(s) 658, 756, 1218
ScrFI CCNGG 5 cut(s) 567, 881, 1599, 1706, 1874
SduI GDGCHC 2 cut(s) 1031, 1656
SfaNI GCATC 8 cut(s) 14, 115, 442, 579, 645, 1089, 1342, 1691
SfoI GGCGCC 1 cut(s) 1284
Sfr274I CTCGAG 1 cut(s) 1515
SfuI TTCGAA 1 cut(s) 1951
SinI GGWCC 4 cut(s) 917, 965, 1679, 1888
SlaI CTCGAG 1 cut(s) 1515
SmiMI CAYNNNNRTG 4 cut(s) 369, 510, 1157, 1404
SmlI CTYRAG 2 cut(s) 1515, 1793
SmoI CTYRAG 2 cut(s) 1515, 1793
SseBI AGGCCT 1 cut(s) 1119
SsiI CCGC 3 cut(s) 1084, 1291, 1798
SspDI GGCGCC 1 cut(s) 1282
SspMI CTAG 3 cut(s) 597, 791, 1179
SstI GAGCTC 1 cut(s) 1656
StuI AGGCCT 1 cut(s) 1119
StyD4I CCNGG 5 cut(s) 565, 879, 1597, 1704, 1872
StyI CCWWGG 5 cut(s) 162, 430, 675, 1133, 1614
TaaI ACNGT 5 cut(s) 237, 412, 1336, 1544, 1822
TaiI ACGT 2 cut(s) 463, 470
TaqI TCGA 5 cut(s) 312, 1076, 1212, 1516, 1951
TfiI GAWTC 2 cut(s) 1163, 1787
Tru1I TTAA 7 cut(s) 65, 720, 1001, 1367, 1473, 1587, 1794
Tru9I TTAA 7 cut(s) 65, 720, 1001, 1367, 1473, 1587, 1794
TscAI CASTG 2 cut(s) 235, 1898
TseFI GTSAC 3 cut(s) 1413, 1723, 1880
Tsp45I GTSAC 3 cut(s) 1413, 1723, 1880
TspDTI ATGAA 6 cut(s) 228, 387, 851, 1244, 1915, 1920
TspRI CASTG 2 cut(s) 235, 1898
Tth111I GACNNNGTC 1 cut(s) 1886
Van91I CCANNNNNTGG 1 cut(s) 1139
Vha464I CTTAAG 1 cut(s) 1793
VpaK11BI GGWCC 4 cut(s) 917, 965, 1679, 1888
VspI ATTAAT 1 cut(s) 720
XapI RAATTY 3 cut(s) 10, 384, 1922
XceI RCATGY 3 cut(s) 509, 1403, 1461
XcmI CCANNNNNNNNNTGG 1 cut(s) 428
XhoI CTCGAG 1 cut(s) 1515
XmiI GTMKAC 2 cut(s) 312, 1076
XspI CTAG 3 cut(s) 597, 791, 1179
ZraI GACGTC 1 cut(s) 468
Zsp2I ATGCAT 1 cut(s) 1357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.