Rmu_sc0003727.1_g000001

acyl-activating enzyme 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003727.1
OG0005780
Physical Location & Seq
Forward (+)
4 .. 3479
3476 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1479 bp
atgacactaaaagaacttcgggaacaagtaatgttggtggctaatgcactagaccaggaggcaaccttttcaaagggtgatgccatcgcaattgacatgccaatgacagtcagtgcagttatcatatatttggcaatcatactagcaggatatgtagttgtatcaatagctgacagtttcgctgctaacgaaattgcaactcgtctgcgtgtgtctaaagcaaagggaatctttacccaggatttcataatacgaggaggtcgtaaatttcctctgtacagtcgggttgtggatgctgctccatgtaaagctattgtgcttccagcaattgggaacagtgtaggcattcaattacgagaacaggatatatcctggaatgactttctgtctagtgtcaatcatctgccgagaaagaataactacgctccagtctatcagccaatagactccatgacaaatattctcttttcatcaggaactacaggagatccaaaagctatcccatggacacaactttcgccaattcgatgtgcagctgattcatgggctcacattgatactcaagttggagatgtcttctgctggcccacaaatttaggatgggttatgggtccagttttaatctactcatgctttctaagtggtgcaactcttgctctctatcatggatctcctctaggtcgtggcttcggaaagtttgttcaggatgcaggagtcactattttgggtacagttcccagcttagtaaaagcttggaagagtacagagtgtatgaaaggcctagattggacaaagataaaatcattcgcttcgactggtgaagcatctaacgtcgatgacgacctttggctctcttcccgtgcttattacaagcccatccttgaatgctgtggaggcacagaacttgcttcatcctacataatggcaagtccactgcagccacaagcttttgggacctttagcacaaagtcgatgacaacaggctttgttatccttgatgaaagtggagtttcttacccagatgatgaagcttgtgttggggaagtgggtttattccctctgtacatgggagcaactgatagattgctaaatgctgaccatgaagaagtctacttcaagggaatgccaatgtacaatggaatgcaactcaggagacatggagacataatcaaaagaacagttgggggctatttcattgttcaaggcagggctgatgacaccatgaaccttggtggcatcaagacaagttctattgaaattgagcgtgtatgtgaccggtctgatgaaagtatcttggagacagctgcagttagtgttgcaccagtgaatgggggtccggaacagttggttatatacgtggtattaaagaagggatttgagaccaaagcagacgagctaaagacgaaattctcaagagccattcatagcaaccttaatcctttgttcaaggtatcaatacctctaatagttcctatttag

Protein Analysis

492

Amino Acids

53.6

Weight (kDa)

5.65

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 1 - 349 5.3e-38 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 329, 1328
AccI GTMKAC 1 cut(s) 1110
AccIII TCCGGA 1 cut(s) 1336
AclWI GGATC 2 cut(s) 482, 676
AcsI RAATTY 3 cut(s) 266, 592, 1406
AfaI GTAC 5 cut(s) 278, 730, 763, 1064, 1133
AfiI CCNNNNNNNGG 2 cut(s) 329, 1328
AgeI ACCGGT 1 cut(s) 1275
AgsI TTSAA 7 cut(s) 72, 350, 884, 1117, 1202, 1256, 1447
AhdI GACNNNNNGTC 1 cut(s) 385
AjnI CCWGG 3 cut(s) 54, 237, 371
AjuI GAANNNNNNNTTGG 2 cut(s) 1020, 1052
AloI GAACNNNNNNTCC 2 cut(s) 684, 716
Alw26I GTCTC 4 cut(s) 1147, 1155, 1292, 1373
AlwI GGATC 2 cut(s) 482, 676
Aor13HI TCCGGA 1 cut(s) 1336
AoxI GGCC 2 cut(s) 584, 778
ApeKI GCWGC 5 cut(s) 182, 296, 533, 937, 1304
ApoI RAATTY 3 cut(s) 266, 592, 1406
ArsI GACNNNNNNTTYG 2 cut(s) 499, 531
AsiGI ACCGGT 1 cut(s) 1275
AspS9I GGNCC 4 cut(s) 585, 611, 954, 1334
AsuHPI GGTGA 2 cut(s) 89, 830
AvaII GGWCC 3 cut(s) 611, 954, 1334
BaeI ACNNNNGTAYC 2 cut(s) 549, 582
BanII GRGCYC 1 cut(s) 550
BbsI GAAGAC 1 cut(s) 568
BbvI GCAGC 5 cut(s) 169, 283, 545, 949, 1291
BccI CCATC 3 cut(s) 92, 594, 884
BciT130I CCWGG 3 cut(s) 56, 239, 373
BcoDI GTCTC 4 cut(s) 1147, 1155, 1292, 1373
BfaI CTAG 5 cut(s) 50, 143, 390, 677, 782
BfmI CTRYAG 3 cut(s) 480, 935, 1305
BisI GCNGC 5 cut(s) 183, 297, 534, 938, 1305
BlsI GCNGC 5 cut(s) 184, 298, 535, 939, 1306
Bme1390I CCNGG 3 cut(s) 56, 239, 373
Bme18I GGWCC 3 cut(s) 611, 954, 1334
BmeRI GACNNNNNGTC 1 cut(s) 385
BmgT120I GGNCC 4 cut(s) 585, 611, 954, 1334
BmiI GGNNCC 3 cut(s) 612, 955, 1335
BmrFI CCNGG 3 cut(s) 56, 239, 373
BmsI GCATC 5 cut(s) 70, 283, 697, 833, 1245
BpiI GAAGAC 1 cut(s) 568
BpmI CTGGAG 1 cut(s) 411
BpuEI CTTGAG 2 cut(s) 546, 1396
BsaAI YACGTR 1 cut(s) 1357
BsaI GGTCTC 1 cut(s) 1373
BsaJI CCNNGG 3 cut(s) 237, 503, 1228
BsaWI WCCGGW 2 cut(s) 1275, 1336
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 1328
Bse118I RCCGGY 1 cut(s) 1275
Bse1I ACTGG 4 cut(s) 428, 614, 820, 1322
BseAI TCCGGA 1 cut(s) 1336
BseBI CCWGG 3 cut(s) 56, 239, 373
BseDI CCNNGG 3 cut(s) 237, 503, 1228
BseGI GGATG 5 cut(s) 298, 605, 712, 876, 911
BseLI CCNNNNNNNGG 2 cut(s) 329, 1328
BseMII CTCAG 1 cut(s) 1162
BseNI ACTGG 4 cut(s) 428, 614, 820, 1322
BseRI GAGGAG 2 cut(s) 270, 663
BseXI GCAGC 5 cut(s) 169, 283, 545, 949, 1291
BseYI CCCAGC 1 cut(s) 737
BsgI GTGCAG 2 cut(s) 135, 552
BshFI GGCC 2 cut(s) 586, 780
BshTI ACCGGT 1 cut(s) 1275
BsiSI CCGG 2 cut(s) 1276, 1337
BslFI GGGAC 1 cut(s) 967
BslI CCNNNNNNNGG 2 cut(s) 329, 1328
BsmAI GTCTC 4 cut(s) 1147, 1155, 1292, 1373
BsmFI GGGAC 1 cut(s) 967
BsmI GAATGC 4 cut(s) 345, 890, 1128, 1146
BsnI GGCC 2 cut(s) 586, 780
Bso31I GGTCTC 1 cut(s) 1373
Bsp1286I GDGCHC 1 cut(s) 550
Bsp13I TCCGGA 1 cut(s) 1336
Bsp1407I TGTACA 3 cut(s) 276, 1062, 1131
Bsp143I GATC 2 cut(s) 487, 668
Bsp19I CCATGG 1 cut(s) 503
BspANI GGCC 2 cut(s) 586, 780
BspCNI CTCAG 1 cut(s) 1161
BspEI TCCGGA 1 cut(s) 1336
BspLI GGNNCC 3 cut(s) 612, 955, 1335
BspMAI CTGCAG 2 cut(s) 939, 1309
BspPI GGATC 2 cut(s) 482, 676
BspTNI GGTCTC 1 cut(s) 1373
BsrFI RCCGGY 1 cut(s) 1275
BsrGI TGTACA 3 cut(s) 276, 1062, 1131
BsrI ACTGG 4 cut(s) 428, 614, 820, 1322
BssAI RCCGGY 1 cut(s) 1275
BssECI CCNNGG 3 cut(s) 237, 503, 1228
BssMI GATC 2 cut(s) 487, 668
BssT1I CCWWGG 2 cut(s) 503, 1228
Bst2UI CCWGG 3 cut(s) 56, 239, 373
Bst4CI ACNGT 7 cut(s) 109, 176, 281, 338, 733, 1180, 1344
Bst6I CTCTTC 2 cut(s) 752, 859
BstAPI GCANNNNNTGC 1 cut(s) 653
BstAUI TGTACA 3 cut(s) 276, 1062, 1131
BstBAI YACGTR 1 cut(s) 1357
BstC8I GCNNGC 1 cut(s) 584
BstDEI CTNAG 3 cut(s) 638, 742, 1148
BstDSI CCRYGG 1 cut(s) 503
BstF5I GGATG 5 cut(s) 298, 605, 712, 876, 911
BstKTI GATC 2 cut(s) 490, 671
BstMAI GTCTC 4 cut(s) 1147, 1155, 1292, 1373
BstMBI GATC 2 cut(s) 487, 668
BstMWI GCNNNNNNNGC 2 cut(s) 653, 894
BstNI CCWGG 3 cut(s) 56, 239, 373
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 3 cut(s) 54, 237, 371
BstSFI CTRYAG 3 cut(s) 480, 935, 1305
BstV1I GCAGC 5 cut(s) 169, 283, 545, 949, 1291
BstV2I GAAGAC 1 cut(s) 568
BstX2I RGATCY 2 cut(s) 487, 668
BstYI RGATCY 2 cut(s) 487, 668
BsuRI GGCC 2 cut(s) 586, 780
BtgI CCRYGG 1 cut(s) 503
BtgZI GCGATG 1 cut(s) 70
BtsCI GGATG 5 cut(s) 298, 605, 712, 876, 911
BtsI GCAGTG 1 cut(s) 932
BtsIMutI CAGTG 4 cut(s) 118, 343, 932, 1329
Cac8I GCNNGC 1 cut(s) 584
Cfr10I RCCGGY 1 cut(s) 1275
Cfr13I GGNCC 4 cut(s) 585, 611, 954, 1334
Csp6I GTAC 5 cut(s) 277, 729, 762, 1063, 1132
CspAI ACCGGT 1 cut(s) 1275
CviQI GTAC 5 cut(s) 277, 729, 762, 1063, 1132
DdeI CTNAG 3 cut(s) 638, 742, 1148
DpnI GATC 2 cut(s) 489, 670
DpnII GATC 2 cut(s) 487, 668
DriI GACNNNNNGTC 1 cut(s) 385
Eam1104I CTCTTC 2 cut(s) 752, 859
Eam1105I GACNNNNNGTC 1 cut(s) 385
EarI CTCTTC 2 cut(s) 752, 859
Eco130I CCWWGG 2 cut(s) 503, 1228
Eco147I AGGCCT 1 cut(s) 780
Eco24I GRGCYC 1 cut(s) 550
Eco31I GGTCTC 1 cut(s) 1373
Eco47I GGWCC 3 cut(s) 611, 954, 1334
EcoO109I RGGNCCY 1 cut(s) 954
EcoRII CCWGG 3 cut(s) 54, 237, 371
EcoT14I CCWWGG 2 cut(s) 503, 1228
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 2 cut(s) 503, 1228
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FaqI GGGAC 1 cut(s) 967
FblI GTMKAC 1 cut(s) 1110
Fnu4HI GCNGC 5 cut(s) 183, 297, 534, 938, 1305
FokI GGATG 5 cut(s) 305, 612, 719, 863, 898
FriOI GRGCYC 1 cut(s) 550
Fsp4HI GCNGC 5 cut(s) 183, 297, 534, 938, 1305
FspBI CTAG 5 cut(s) 50, 143, 390, 677, 782
GluI GCNGC 5 cut(s) 183, 297, 534, 938, 1305
GsaI CCCAGC 1 cut(s) 741
GsuI CTGGAG 1 cut(s) 411
HaeIII GGCC 2 cut(s) 586, 780
HapII CCGG 2 cut(s) 1276, 1337
HindIII AAGCTT 3 cut(s) 750, 945, 1029
HinfI GANTC 4 cut(s) 228, 446, 539, 714
HpaII CCGG 2 cut(s) 1276, 1337
HphI GGTGA 2 cut(s) 89, 830
Hpy166II GTNNAC 2 cut(s) 932, 1111
Hpy188I TCNGA 2 cut(s) 692, 1282
Hpy188III TCNNGA 7 cut(s) 20, 474, 704, 1150, 1240, 1337, 1413
Hpy8I GTNNAC 2 cut(s) 932, 1111
Hpy99I CGWCG 1 cut(s) 836
HpyAV CCTTC 1 cut(s) 1363
HpyCH4III ACNGT 7 cut(s) 109, 176, 281, 338, 733, 1180, 1344
HpyCH4IV ACGT 2 cut(s) 831, 1356
HpyF10VI GCNNNNNNNGC 2 cut(s) 653, 894
HpyF3I CTNAG 3 cut(s) 638, 742, 1148
HpySE526I ACGT 2 cut(s) 831, 1356
Kpn2I TCCGGA 1 cut(s) 1336
Kzo9I GATC 2 cut(s) 487, 668
LmnI GCTCC 3 cut(s) 304, 430, 1070
Lsp1109I GCAGC 5 cut(s) 169, 283, 545, 949, 1291
LweI GCATC 5 cut(s) 70, 283, 697, 833, 1245
MaeI CTAG 5 cut(s) 50, 143, 390, 677, 782
MaeII ACGT 2 cut(s) 831, 1356
MaeIII GTNAC 2 cut(s) 715, 1271
MalI GATC 2 cut(s) 489, 670
MboI GATC 2 cut(s) 487, 668
MboII GAAGA 4 cut(s) 568, 769, 846, 1115
MfeI CAATTG 2 cut(s) 90, 327
MflI RGATCY 2 cut(s) 487, 668
MhlI GDGCHC 1 cut(s) 550
MluCI AATT 9 cut(s) 90, 192, 266, 327, 350, 522, 592, 1257, 1406
MlyI GAGTC 2 cut(s) 440, 723
MmeI TCCRAC 1 cut(s) 547
MnlI CCTC 8 cut(s) 52, 248, 251, 282, 684, 887, 1068, 1470
MroI TCCGGA 1 cut(s) 1336
MseI TTAA 3 cut(s) 620, 1364, 1434
MslI CAYNNNNRTG 1 cut(s) 101
MspA1I CMGCKG 2 cut(s) 536, 1304
MspI CCGG 2 cut(s) 1276, 1337
MspR9I CCNGG 3 cut(s) 56, 239, 373
MunI CAATTG 2 cut(s) 90, 327
Mva1269I GAATGC 4 cut(s) 345, 890, 1128, 1146
MvaI CCWGG 3 cut(s) 56, 239, 373
MwoI GCNNNNNNNGC 2 cut(s) 653, 894
NcoI CCATGG 1 cut(s) 503
NdeII GATC 2 cut(s) 487, 668
NlaIV GGNNCC 3 cut(s) 612, 955, 1335
NmeAIII GCCGAG 1 cut(s) 432
NmuCI GTSAC 2 cut(s) 715, 1271
NspI RCATGY 1 cut(s) 100
PceI AGGCCT 1 cut(s) 780
PcsI WCGNNNNNNNCGW 3 cut(s) 186, 259, 837
PctI GAATGC 4 cut(s) 345, 890, 1128, 1146
PfeI GAWTC 2 cut(s) 228, 539
PflMI CCANNNNNTGG 2 cut(s) 329, 1328
PfoI TCCNGGA 1 cut(s) 371
PinAI ACCGGT 1 cut(s) 1275
PkrI GCNGC 5 cut(s) 184, 298, 535, 939, 1306
PleI GAGTC 2 cut(s) 440, 722
PpsI GAGTC 2 cut(s) 440, 722
Ppu21I YACGTR 1 cut(s) 1357
PpuMI RGGWCCY 1 cut(s) 954
Psp5II RGGWCCY 1 cut(s) 954
Psp6I CCWGG 3 cut(s) 54, 237, 371
PspFI CCCAGC 1 cut(s) 737
PspGI CCWGG 3 cut(s) 54, 237, 371
PspN4I GGNNCC 3 cut(s) 612, 955, 1335
PspPI GGNCC 4 cut(s) 585, 611, 954, 1334
PspPPI RGGWCCY 1 cut(s) 954
PstI CTGCAG 2 cut(s) 939, 1309
PsuI RGATCY 2 cut(s) 487, 668
PvuII CAGCTG 2 cut(s) 536, 1304
RsaI GTAC 5 cut(s) 278, 730, 763, 1064, 1133
RsaNI GTAC 5 cut(s) 277, 729, 762, 1063, 1132
RseI CAYNNNNRTG 1 cut(s) 101
SaqAI TTAA 3 cut(s) 620, 1364, 1434
SatI GCNGC 5 cut(s) 183, 297, 534, 938, 1305
Sau3AI GATC 2 cut(s) 487, 668
Sau96I GGNCC 4 cut(s) 585, 611, 954, 1334
SchI GAGTC 2 cut(s) 440, 723
ScrFI CCNGG 3 cut(s) 56, 239, 373
SduI GDGCHC 1 cut(s) 550
SfaNI GCATC 5 cut(s) 70, 283, 697, 833, 1245
SfcI CTRYAG 3 cut(s) 480, 935, 1305
SinI GGWCC 3 cut(s) 611, 954, 1334
SmiMI CAYNNNNRTG 1 cut(s) 101
SmlI CTYRAG 2 cut(s) 561, 1411
SmoI CTYRAG 2 cut(s) 561, 1411
Sse9I AATT 9 cut(s) 90, 192, 266, 327, 350, 522, 592, 1257, 1406
SseBI AGGCCT 1 cut(s) 780
SspI AATATT 1 cut(s) 460
SspMI CTAG 5 cut(s) 50, 143, 390, 677, 782
StuI AGGCCT 1 cut(s) 780
StyD4I CCNGG 3 cut(s) 54, 237, 371
StyI CCWWGG 2 cut(s) 503, 1228
TaaI ACNGT 7 cut(s) 109, 176, 281, 338, 733, 1180, 1344
TaiI ACGT 2 cut(s) 834, 1359
TaqI TCGA 4 cut(s) 526, 812, 834, 971
TasI AATT 9 cut(s) 90, 192, 266, 327, 350, 522, 592, 1257, 1406
TatI WGTACW 4 cut(s) 276, 761, 1062, 1131
TfiI GAWTC 2 cut(s) 228, 539
Tru1I TTAA 3 cut(s) 620, 1364, 1434
Tru9I TTAA 3 cut(s) 620, 1364, 1434
TscAI CASTG 4 cut(s) 118, 343, 939, 1329
TseFI GTSAC 2 cut(s) 715, 1271
TseI GCWGC 5 cut(s) 182, 296, 533, 937, 1304
Tsp45I GTSAC 2 cut(s) 715, 1271
TspRI CASTG 4 cut(s) 118, 343, 939, 1329
Van91I CCANNNNNTGG 2 cut(s) 329, 1328
VpaK11BI GGWCC 3 cut(s) 611, 954, 1334
XapI RAATTY 3 cut(s) 266, 592, 1406
XceI RCATGY 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 1110
XspI CTAG 5 cut(s) 50, 143, 390, 677, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.