Rmu_sc0003823.1_g000008

Methionine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003823.1
OG0015103
Physical Location & Seq
Forward (+)
44959 .. 55235
10277 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3447 bp
atggcgaagaacgacgcgtcgtcgtcggcgttgggatcggtggacgagttcctgaagcggtgcgagcaatccggcgatgccgcgtactctgctctgagatcagtgctggagcgtctcgaggatccgaaaacccgaactcaagctcggatcttcctctccagcctccagaagcgcttccctaccaaggacgactgcgatcactgcttccgcacctatcatttccagattgaggacatcttcttcgatcagtatcaaggttaccagggtaggaagaagctgacaatgatggttatccctagcatttttgttccagaagattggtccttcacttttttcgaaggactaaatagacatccggactctatctttaaggacaagtcactcgctgagcttggctgtggaaatggttggatatccattgccattgctgagaagtggttgccatccaaggtgtatggactcgatatcaatcctagagcagtcaagatgtcatggataaatttgtatctaaatgctttggacgaggaaggtcagcccatctatgatgctgagaaaaagaccttgctggacagggttgagtttcatgaatcagatctcctatcttattgcagagacaatgatattcaacttgaacgaattgttggttgcatccctcagattcttaacccgaatccagatgcaatgtctaagatgataactgagaatgcaagtgaggaatttctacattcattgagcaattattgtgcgcttcagggttttcttgaggatcagtttggcttaggtctaattgctagggcagtggaggaaggaatagctgtcatcaaacctatggggataatgatcttcaacatgggagggcgtccaggacaagctgtttgtaagcgcttgtttgaacgccgtggctttcatgttaacaagctttggcagactaaaattcttcaggcaagtgtacccttctttgtttggggttttcttgaggatcagtttggcttaggtctaattgctagggcagtggaggaaggaatagctgtcatcaaacctatggggataatgatcttcaacatgggagggcgtccaggacaagctgtttgtaagcgcttgtttgaacgccgtggctttcatgttaacaagctttggcagactaaaattcttcaggctgcagacacagatatatcagcattagttgaaattgaaaagaacagtccacaccgttttgagttcttcatgggactttctggagatcagcctatttgtgctcgaacagcatgggcatatggaaatgccggtggtcgaatctcgcacgctttatcagtttacagctgtcaactccgccaaccaaatcaggtcaagacaatttttgagtttcttaagaatggcttccacgacatcagtagttctttagatttatcgtttgaagatgattcagtggctgatgagaagattcccttcctagcttatctttccagtgttctgaaagggagttctttctgcaagtatgaaccaccagctggaagcaaacattttcgcaacctcattgctggggctgtagcaatagaaaatgctcttcggttgttctcacctcgtcttgcaattgttgatgaacatctgacgcgccaccttccaaggaactggttaacatctttagcagttaagtgtgcaggaactgataatcctgcagaggattcacttacagttattgaagcgcctcgacagtctgatttgataatagagcttataaggaagttgaagccacaggtggtggttactgggatagcggattatgagtctgttactagttcagcctttgtacaccttttagatgttacaagagaagttggatctcgtcttttcttagacatatctgatcattttgagctatccagccttccaagttccaatggagtcctcaaatatatagggggaaatgttctgccatcccatgcagcaattatatgtggcctagtgaaaaacaaggtttattcagatctcgaagtagcttttgtaatttcagaggaggaaaatattttcaaggccttgtcaaagactgttgaactactagaagggagtacagcaccaattagtcaaggttattatggttgtctttttcatgaacttttagcttttcaacttgctgacagacatccacctccacagagggaatgcacatcagttaaatcagcagagatgattggttttgctagttcagcaaactcggtccttaataatgctgagttggcaattaatgaggcagggaactcttccctgattcatatggacgtggatcaaagcttcttacatgtaccatcacctgtcaatgctgcaatttttgaaagctttgccagacagaacatagctgagtcagaaatagatgtcaccactagcattaaggaattcataaagagcaattatggttacccacttgatagcaacaccgaatttatatatgctgacagctcgctagctctgtttaacaagttggttctttgttgcatacaagaaggggggacattgtgctttccttctggctcaaatggaaactatgtctctgctgcaaaatttttgaaagcaaatattgtaaatatacctacaaagatcgaagaaggctttaaactgacagaaaagggtctttctggagtcctagagaccctggataaaccatgggtatacatttctggaccaacaattaacccaactggtgcgctatacaacaacaaagagattgaaaatttactgtctacctgtgccaaatttggggctagggttgtgattgacacttcattctcgggtttggaatttgaccctgagggttggggtggctggagtctggtggacagtttggcaaagttatattcttctagcaaaccctctttttgtgtctctctgctcgggggattgtctttgaagatgctgagtgggggactcaaatttgggtttctggttctgaaccagtctgctgtggtggaaacattttatagctttccaggattaagcaaacctcataacactgttaaatatgccgttaagaagctactgggtctgagagagcagaaatcaggagatctttgggatgccattgcagaacagataagaaatttgaagagtcgatccaagcgcttgaaggagacactggagaagtgtggatgggatgtgcttgaatctagtggtggtgtttccatggtggccaagccctcatcttatttgaataagtctgttaagttcaagcaatataaagatggaggcagcactgaagatggaacagtgcatgaagttaagcttgatgactctaatatcagggaggtcgttcacaagtctactggcttatgcatcaatagtggctcctggactggaattcctggctattgtcgtttcaccattgctcttgaagaaagtgagtttgagcgggcattggattgcattgttcaatttaagaagaccatcagtaactga

Protein Analysis

1148

Amino Acids

127.29

Weight (kDa)

5.85

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 790 - 1138 1.3e-19 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49810
fragaria_vesca FvH4_5g27550
malus_domestica MD08G1249200.v1.1 MD15G1443800.v1.1
prunus_persica Prupe.1G586400_v2.0.a1
pyrus_communis pycom08g21570
rosa_chinensis RchiOBHm_Chr7g0218921
rosa_laevigata RLG00000002317
rosa_multiflora Rmu_sc0003823.1_g000008
rosa_roxburghii Rroxscaffold_3G00240310
rosa_rugosa Rorug07G0179800
rosa_samantha Rh7AG323100 Rh7BG314100 Rh7CG339800 Rh7DG320200
rosa_wichuraiana Rw7G027380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1736
AccB7I CCANNNNNTGG 1 cut(s) 1508
AccBSI CCGCTC 1 cut(s) 3400
AccI GTMKAC 3 cut(s) 2664, 2735, 3311
AccII CGCG 3 cut(s) 17, 83, 1612
AccIII TCCGGA 1 cut(s) 355
AciI CCGC 6 cut(s) 58, 81, 208, 1330, 1775, 3400
AclWI GGATC 9 cut(s) 43, 116, 129, 155, 774, 987, 1846, 2289, 3099
AcoI YGGCCR 1 cut(s) 3180
AcuI CTGAAG 5 cut(s) 74, 734, 923, 1136, 3267
AcyI GRCGYC 2 cut(s) 859, 1072
AfaI GTAC 5 cut(s) 86, 951, 1809, 2068, 2301
AfeI AGCGCT 4 cut(s) 173, 884, 1097, 3113
AfiI CCNNNNNNNGG 4 cut(s) 184, 229, 1508, 2751
AflII CTTAAG 1 cut(s) 1367
AflIII ACRYGT 2 cut(s) 15, 2296
AhdI GACNNNNNGTC 1 cut(s) 19
AhlI ACTAGT 1 cut(s) 1793
AjiI CACGTC 1 cut(s) 2278
AjnI CCWGG 7 cut(s) 261, 862, 1075, 2646, 2980, 3338, 3352
AjuI GAANNNNNNNTTGG 2 cut(s) 624, 656
AloI GAACNNNNNNTCC 2 cut(s) 1654, 1686
Alw21I GWGCWC 1 cut(s) 1258
Alw26I GTCTC 6 cut(s) 119, 606, 2548, 2636, 2881, 3116
AlwI GGATC 9 cut(s) 43, 116, 129, 155, 774, 987, 1846, 2289, 3099
AlwNI CAGNNNCTG 3 cut(s) 1430, 1664, 3444
Ama87I CYCGRG 3 cut(s) 116, 2782, 2885
Aor13HI TCCGGA 1 cut(s) 355
Aor51HI AGCGCT 4 cut(s) 173, 884, 1097, 3113
AoxI GGCC 3 cut(s) 1957, 2031, 3180
ApeKI GCWGC 5 cut(s) 1157, 1943, 2318, 2549, 3240
ArsI GACNNNNNNTTYG 6 cut(s) 224, 256, 858, 890, 1071, 1103
AseI ATTAAT 1 cut(s) 2241
Asp700I GAANNNNTTC 2 cut(s) 173, 1376
AspLEI GCGC 8 cut(s) 174, 748, 885, 1098, 1614, 1705, 2701, 3114
AspS9I GGNCC 3 cut(s) 321, 2215, 2675
AsuHPI GGTGA 4 cut(s) 1569, 2298, 2365, 3361
AsuII TTCGAA 1 cut(s) 336
AsuNHI GCTAGC 1 cut(s) 2458
AvaI CYCGRG 3 cut(s) 116, 2782, 2885
AvaII GGWCC 3 cut(s) 321, 2215, 2675
AxyI CCTNAGG 1 cut(s) 2802
BalI TGGCCA 1 cut(s) 3182
BamHI GGATCC 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 3437
Bbv12I GWGCWC 1 cut(s) 1258
BbvI GCAGC 5 cut(s) 1144, 1955, 2305, 2536, 3252
BccI CCATC 9 cut(s) 280, 451, 545, 1942, 2311, 3135, 3227, 3245, 3443
BceAI ACGGC 3 cut(s) 882, 1095, 3002
BciT130I CCWGG 7 cut(s) 263, 864, 1077, 2648, 2982, 3340, 3354
BclI TGATCA 1 cut(s) 1864
BcoDI GTCTC 6 cut(s) 119, 606, 2548, 2636, 2881, 3116
BcuI ACTAGT 1 cut(s) 1793
BfmI CTRYAG 3 cut(s) 1158, 1545, 1674
BfoI RGCGCY 5 cut(s) 175, 886, 1099, 1706, 3115
BfrI CTTAAG 1 cut(s) 1367
BglII AGATCT 3 cut(s) 592, 1984, 3058
BisI GCNGC 6 cut(s) 81, 1158, 1944, 2319, 2550, 3241
BlpI GCTNAGC 1 cut(s) 387
BlsI GCNGC 6 cut(s) 82, 1159, 1945, 2320, 2551, 3242
Bme1390I CCNGG 7 cut(s) 263, 864, 1077, 2648, 2982, 3340, 3354
Bme18I GGWCC 3 cut(s) 321, 2215, 2675
BmeRI GACNNNNNGTC 1 cut(s) 19
BmeT110I CYCGRG 3 cut(s) 116, 2782, 2885
BmgBI CACGTC 1 cut(s) 2278
BmgT120I GGNCC 3 cut(s) 321, 2215, 2675
BmiI GGNNCC 2 cut(s) 123, 3337
BmrFI CCNGG 7 cut(s) 263, 864, 1077, 2648, 2982, 3340, 3354
BmrI ACTGGG 2 cut(s) 1776, 3041
BmsI GCATC 7 cut(s) 67, 535, 657, 667, 2895, 3058, 3333
BmtI GCTAGC 1 cut(s) 2462
BmuI ACTGGG 2 cut(s) 1776, 3041
BpiI GAAGAC 1 cut(s) 3437
BpmI CTGGAG 7 cut(s) 128, 142, 149, 1257, 2652, 2839, 3149
Bpu10I CCTNAGC 2 cut(s) 778, 991
Bpu1102I GCTNAGC 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 336
BpuEI CTTGAG 3 cut(s) 123, 782, 995
BsaBI GATNNNNATC 6 cut(s) 249, 290, 468, 839, 1052, 1602
BsaHI GRCGYC 2 cut(s) 859, 1072
BsaI GGTCTC 1 cut(s) 2636
BsaJI CCNNGG 9 cut(s) 183, 262, 447, 898, 1111, 1622, 2646, 2657, 3174
BsaWI WCCGGW 1 cut(s) 355
Bsc4I CCNNNNNNNGG 4 cut(s) 184, 229, 1508, 2751
Bse118I RCCGGY 1 cut(s) 1283
Bse1I ACTGG 9 cut(s) 1464, 1634, 1771, 2698, 2947, 3036, 3132, 3319, 3349
Bse21I CCTNAGG 1 cut(s) 2802
Bse3DI GCAATG 6 cut(s) 417, 423, 687, 1533, 3072, 3372
Bse8I GATNNNNATC 6 cut(s) 249, 290, 468, 839, 1052, 1602
BseAI TCCGGA 1 cut(s) 355
BseBI CCWGG 7 cut(s) 263, 864, 1077, 2648, 2982, 3340, 3354
BseDI CCNNGG 9 cut(s) 183, 262, 447, 898, 1111, 1622, 2646, 2657, 3174
BseGI GGATG 8 cut(s) 352, 443, 648, 1934, 2140, 3073, 3146, 3151
BseJI GATNNNNATC 6 cut(s) 249, 290, 468, 839, 1052, 1602
BseLI CCNNNNNNNGG 4 cut(s) 184, 229, 1508, 2751
BseMI GCAATG 6 cut(s) 417, 423, 687, 1533, 3072, 3372
BseNI ACTGG 9 cut(s) 1464, 1634, 1771, 2698, 2947, 3036, 3132, 3319, 3349
BseRI GAGGAG 1 cut(s) 2027
BseXI GCAGC 5 cut(s) 1144, 1955, 2305, 2536, 3252
BseYI CCCAGC 1 cut(s) 1538
BsgI GTGCAG 1 cut(s) 1677
Bsh1236I CGCG 3 cut(s) 17, 83, 1612
BshFI GGCC 3 cut(s) 1959, 2033, 3182
BsiHKAI GWGCWC 1 cut(s) 1258
BsiHKCI CYCGRG 3 cut(s) 116, 2782, 2885
BsiSI CCGG 3 cut(s) 72, 356, 1284
BslFI GGGAC 3 cut(s) 1242, 2518, 2931
BslI CCNNNNNNNGG 4 cut(s) 184, 229, 1508, 2751
BsmAI GTCTC 6 cut(s) 119, 606, 2548, 2636, 2881, 3116
BsmBI CGTCTC 1 cut(s) 119
BsmFI GGGAC 3 cut(s) 1242, 2518, 2931
BsmI GAATGC 2 cut(s) 709, 2165
BsnI GGCC 3 cut(s) 1959, 2033, 3182
Bso31I GGTCTC 1 cut(s) 2636
BsoBI CYCGRG 3 cut(s) 116, 2782, 2885
Bsp119I TTCGAA 1 cut(s) 336
Bsp1286I GDGCHC 1 cut(s) 1258
Bsp13I TCCGGA 1 cut(s) 355
Bsp1407I TGTACA 1 cut(s) 1807
Bsp1720I GCTNAGC 1 cut(s) 387
Bsp19I CCATGG 2 cut(s) 2657, 3174
BspACI CCGC 6 cut(s) 58, 81, 208, 1330, 1775, 3400
BspANI GGCC 3 cut(s) 1959, 2033, 3182
BspEI TCCGGA 1 cut(s) 355
BspFNI CGCG 3 cut(s) 17, 83, 1612
BspHI TCATGA 2 cut(s) 583, 2107
BspLI GGNNCC 2 cut(s) 123, 3337
BspMAI CTGCAG 2 cut(s) 1162, 1678
BspOI GCTAGC 1 cut(s) 2462
BspPI GGATC 9 cut(s) 43, 116, 129, 155, 774, 987, 1846, 2289, 3099
BspQI GCTCTTC 1 cut(s) 1569
BspT104I TTCGAA 1 cut(s) 336
BspTI CTTAAG 1 cut(s) 1367
BspTNI GGTCTC 1 cut(s) 2636
BsrBI CCGCTC 1 cut(s) 3400
BsrDI GCAATG 6 cut(s) 417, 423, 687, 1533, 3072, 3372
BsrFI RCCGGY 1 cut(s) 1283
BsrGI TGTACA 1 cut(s) 1807
BsrI ACTGG 9 cut(s) 1464, 1634, 1771, 2698, 2947, 3036, 3132, 3319, 3349
BssAI RCCGGY 1 cut(s) 1283
BssECI CCNNGG 9 cut(s) 183, 262, 447, 898, 1111, 1622, 2646, 2657, 3174
BssNAI GTATAC 1 cut(s) 2665
BssNI GRCGYC 2 cut(s) 859, 1072
BssT1I CCWWGG 5 cut(s) 183, 447, 1622, 2657, 3174
Bst1107I GTATAC 1 cut(s) 2665
Bst2UI CCWGG 7 cut(s) 263, 864, 1077, 2648, 2982, 3340, 3354
Bst4CI ACNGT 9 cut(s) 1202, 1211, 1693, 1713, 2047, 2733, 2834, 3007, 3259
Bst6I CTCTTC 3 cut(s) 1569, 2263, 3092
BstACI GRCGYC 2 cut(s) 859, 1072
BstAFI CTTAAG 1 cut(s) 1367
BstAUI TGTACA 1 cut(s) 1807
BstBI TTCGAA 1 cut(s) 336
BstC8I GCNNGC 5 cut(s) 65, 1302, 2456, 2460, 3402
BstDSI CCRYGG 4 cut(s) 898, 1111, 2657, 3174
BstEII GGTNACC 2 cut(s) 257, 2411
BstF5I GGATG 8 cut(s) 352, 443, 648, 1934, 2140, 3073, 3146, 3151
BstFNI CGCG 3 cut(s) 17, 83, 1612
BstH2I RGCGCY 5 cut(s) 175, 886, 1099, 1706, 3115
BstHHI GCGC 8 cut(s) 174, 748, 885, 1098, 1614, 1705, 2701, 3114
BstMAI GTCTC 6 cut(s) 119, 606, 2548, 2636, 2881, 3116
BstMWI GCNNNNNNNGC 6 cut(s) 64, 89, 201, 1262, 2204, 2234
BstNI CCWGG 7 cut(s) 263, 864, 1077, 2648, 2982, 3340, 3354
BstNSI RCATGY 1 cut(s) 2300
BstPI GGTNACC 2 cut(s) 257, 2411
BstSCI CCNGG 7 cut(s) 261, 862, 1075, 2646, 2980, 3338, 3352
BstSFI CTRYAG 3 cut(s) 1158, 1545, 1674
BstUI CGCG 3 cut(s) 17, 83, 1612
BstV1I GCAGC 5 cut(s) 1144, 1955, 2305, 2536, 3252
BstV2I GAAGAC 1 cut(s) 3437
BstX2I RGATCY 6 cut(s) 121, 147, 592, 1838, 1984, 3058
BstXI CCANNNNNNTGG 2 cut(s) 318, 1629
BstYI RGATCY 6 cut(s) 121, 147, 592, 1838, 1984, 3058
BstZ17I GTATAC 1 cut(s) 2665
Bsu36I CCTNAGG 1 cut(s) 2802
BsuRI GGCC 3 cut(s) 1959, 2033, 3182
BtgI CCRYGG 4 cut(s) 898, 1111, 2657, 3174
BtgZI GCGATG 1 cut(s) 90
BtrI CACGTC 1 cut(s) 2278
BtsCI GGATG 8 cut(s) 352, 443, 648, 1934, 2140, 3073, 3146, 3151
BtsI GCAGTG 3 cut(s) 199, 804, 1017
Cac8I GCNNGC 5 cut(s) 65, 1302, 2456, 2460, 3402
CaiI CAGNNNCTG 3 cut(s) 1430, 1664, 3444
CciI TCATGA 2 cut(s) 583, 2107
CfoI GCGC 8 cut(s) 174, 748, 885, 1098, 1614, 1705, 2701, 3114
Cfr10I RCCGGY 1 cut(s) 1283
Cfr13I GGNCC 3 cut(s) 321, 2215, 2675
CseI GACGC 6 cut(s) 6, 23, 101, 848, 1061, 1618
Csp6I GTAC 5 cut(s) 85, 950, 1808, 2067, 2300
CviQI GTAC 5 cut(s) 85, 950, 1808, 2067, 2300
DraI TTTAAA 1 cut(s) 2608
DriI GACNNNNNGTC 1 cut(s) 19
EaeI YGGCCR 1 cut(s) 3180
Eam1104I CTCTTC 3 cut(s) 1569, 2263, 3092
Eam1105I GACNNNNNGTC 1 cut(s) 19
EarI CTCTTC 3 cut(s) 1569, 2263, 3092
EciI GGCGGA 1 cut(s) 1319
Eco130I CCWWGG 5 cut(s) 183, 447, 1622, 2657, 3174
Eco147I AGGCCT 1 cut(s) 2033
Eco31I GGTCTC 1 cut(s) 2636
Eco32I GATATC 2 cut(s) 414, 466
Eco47I GGWCC 3 cut(s) 321, 2215, 2675
Eco47III AGCGCT 4 cut(s) 173, 884, 1097, 3113
Eco57I CTGAAG 5 cut(s) 74, 734, 923, 1136, 3267
Eco81I CCTNAGG 1 cut(s) 2802
Eco88I CYCGRG 3 cut(s) 116, 2782, 2885
Eco91I GGTNACC 2 cut(s) 257, 2411
EcoO65I GGTNACC 2 cut(s) 257, 2411
EcoRI GAATTC 2 cut(s) 2390, 3348
EcoRII CCWGG 7 cut(s) 261, 862, 1075, 2646, 2980, 3338, 3352
EcoRV GATATC 2 cut(s) 414, 466
EcoT14I CCWWGG 5 cut(s) 183, 447, 1622, 2657, 3174
EcoT22I ATGCAT 1 cut(s) 3326
ErhI CCWWGG 5 cut(s) 183, 447, 1622, 2657, 3174
Esp3I CGTCTC 1 cut(s) 119
FalI AAGNNNNNCTT 6 cut(s) 1361, 1393, 1430, 1462, 3258, 3290
FaqI GGGAC 3 cut(s) 1242, 2518, 2931
FauI CCCGC 1 cut(s) 3393
FauNDI CATATG 2 cut(s) 1273, 2271
FbaI TGATCA 1 cut(s) 1864
FblI GTMKAC 3 cut(s) 2664, 2735, 3311
Fnu4HI GCNGC 6 cut(s) 81, 1158, 1944, 2319, 2550, 3241
FokI GGATG 8 cut(s) 339, 430, 635, 1921, 2127, 3080, 3153, 3158
Fsp4HI GCNGC 6 cut(s) 81, 1158, 1944, 2319, 2550, 3241
GlaI GCGC 8 cut(s) 173, 747, 884, 1097, 1613, 1704, 2700, 3113
GluI GCNGC 6 cut(s) 81, 1158, 1944, 2319, 2550, 3241
GsaI CCCAGC 1 cut(s) 1542
GsuI CTGGAG 7 cut(s) 128, 142, 149, 1257, 2652, 2839, 3149
HaeII RGCGCY 5 cut(s) 175, 886, 1099, 1706, 3115
HaeIII GGCC 3 cut(s) 1959, 2033, 3182
HapII CCGG 3 cut(s) 72, 356, 1284
HgaI GACGC 6 cut(s) 6, 23, 101, 848, 1061, 1618
HhaI GCGC 8 cut(s) 174, 748, 885, 1098, 1614, 1705, 2701, 3114
Hin1I GRCGYC 2 cut(s) 859, 1072
Hin6I GCGC 8 cut(s) 172, 746, 883, 1096, 1612, 1703, 2699, 3112
HinP1I GCGC 8 cut(s) 172, 746, 883, 1096, 1612, 1703, 2699, 3112
HincII GTYRAC 4 cut(s) 913, 1126, 1325, 1635
HindII GTYRAC 4 cut(s) 913, 1126, 1325, 1635
HindIII AAGCTT 5 cut(s) 917, 1130, 2287, 2332, 3272
HpaI GTTAAC 3 cut(s) 913, 1126, 1635
HpaII CCGG 3 cut(s) 72, 356, 1284
HphI GGTGA 4 cut(s) 1569, 2298, 2365, 3361
Hpy99I CGWCG 4 cut(s) 17, 22, 25, 28
HpyCH4III ACNGT 9 cut(s) 1202, 1211, 1693, 1713, 2047, 2733, 2834, 3007, 3259
HpyCH4IV ACGT 1 cut(s) 2277
HpyF10VI GCNNNNNNNGC 6 cut(s) 64, 89, 201, 1262, 2204, 2234
HpySE526I ACGT 1 cut(s) 2277
Hsp92I GRCGYC 2 cut(s) 859, 1072
HspAI GCGC 8 cut(s) 172, 746, 883, 1096, 1612, 1703, 2699, 3112
Kpn2I TCCGGA 1 cut(s) 355
Ksp22I TGATCA 1 cut(s) 1864
KspAI GTTAAC 3 cut(s) 913, 1126, 1635
LguI GCTCTTC 1 cut(s) 1569
LmnI GCTCC 2 cut(s) 109, 3341
Lsp1109I GCAGC 5 cut(s) 1144, 1955, 2305, 2536, 3252
LweI GCATC 7 cut(s) 67, 535, 657, 667, 2895, 3058, 3333
MaeII ACGT 1 cut(s) 2277
MaeIII GTNAC 8 cut(s) 257, 378, 1762, 1789, 1822, 2371, 2411, 3440
MbiI CCGCTC 1 cut(s) 3400
MfeI CAATTG 1 cut(s) 1590
MflI RGATCY 6 cut(s) 121, 147, 592, 1838, 1984, 3058
MhlI GDGCHC 1 cut(s) 1258
MlsI TGGCCA 1 cut(s) 3182
MluI ACGCGT 1 cut(s) 15
MluNI TGGCCA 1 cut(s) 3182
MmeI TCCRAC 2 cut(s) 389, 1816
Mox20I TGGCCA 1 cut(s) 3182
Mph1103I ATGCAT 1 cut(s) 3326
MroI TCCGGA 1 cut(s) 355
MroXI GAANNNNTTC 2 cut(s) 173, 1376
MscI TGGCCA 1 cut(s) 3182
Msp20I TGGCCA 1 cut(s) 3182
MspA1I CMGCKG 2 cut(s) 1320, 1508
MspCI CTTAAG 1 cut(s) 1367
MspI CCGG 3 cut(s) 72, 356, 1284
MspR9I CCNGG 7 cut(s) 263, 864, 1077, 2648, 2982, 3340, 3354
MunI CAATTG 1 cut(s) 1590
Mva1269I GAATGC 2 cut(s) 709, 2165
MvaI CCWGG 7 cut(s) 263, 864, 1077, 2648, 2982, 3340, 3354
MvnI CGCG 3 cut(s) 17, 83, 1612
MwoI GCNNNNNNNGC 6 cut(s) 64, 89, 201, 1262, 2204, 2234
NcoI CCATGG 2 cut(s) 2657, 3174
NdeI CATATG 2 cut(s) 1273, 2271
NheI GCTAGC 1 cut(s) 2458
NlaIV GGNNCC 2 cut(s) 123, 3337
NmuCI GTSAC 2 cut(s) 378, 2371
NsiI ATGCAT 1 cut(s) 3326
NspI RCATGY 1 cut(s) 2300
NspV TTCGAA 1 cut(s) 336
PaeR7I CTCGAG 1 cut(s) 116
PagI TCATGA 2 cut(s) 583, 2107
PceI AGGCCT 1 cut(s) 2033
PciI ACATGT 1 cut(s) 2296
PciSI GCTCTTC 1 cut(s) 1569
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PctI GAATGC 2 cut(s) 709, 2165
PdmI GAANNNNTTC 2 cut(s) 173, 1376
PfeI GAWTC 9 cut(s) 587, 658, 670, 1293, 1421, 1441, 1682, 2266, 3155
PflMI CCANNNNNTGG 1 cut(s) 1508
PfoI TCCNGGA 4 cut(s) 862, 1075, 2980, 3338
PkrI GCNGC 6 cut(s) 82, 1159, 1945, 2320, 2551, 3242
PscI ACATGT 1 cut(s) 2296
PshBI ATTAAT 1 cut(s) 2241
PsiI TTATAA 1 cut(s) 1736
Psp6I CCWGG 7 cut(s) 261, 862, 1075, 2646, 2980, 3338, 3352
PspEI GGTNACC 2 cut(s) 257, 2411
PspFI CCCAGC 1 cut(s) 1538
PspGI CCWGG 7 cut(s) 261, 862, 1075, 2646, 2980, 3338, 3352
PspN4I GGNNCC 2 cut(s) 123, 3337
PspPI GGNCC 3 cut(s) 321, 2215, 2675
PstI CTGCAG 2 cut(s) 1162, 1678
PstNI CAGNNNCTG 3 cut(s) 1430, 1664, 3444
PsuI RGATCY 6 cut(s) 121, 147, 592, 1838, 1984, 3058
PvuII CAGCTG 2 cut(s) 1320, 1508
RsaI GTAC 5 cut(s) 86, 951, 1809, 2068, 2301
RsaNI GTAC 5 cut(s) 85, 950, 1808, 2067, 2300
SapI GCTCTTC 1 cut(s) 1569
SatI GCNGC 6 cut(s) 81, 1158, 1944, 2319, 2550, 3241
Sau96I GGNCC 3 cut(s) 321, 2215, 2675
ScrFI CCNGG 7 cut(s) 263, 864, 1077, 2648, 2982, 3340, 3354
SduI GDGCHC 1 cut(s) 1258
SfaNI GCATC 7 cut(s) 67, 535, 657, 667, 2895, 3058, 3333
SfcI CTRYAG 3 cut(s) 1158, 1545, 1674
Sfr274I CTCGAG 1 cut(s) 116
SfuI TTCGAA 1 cut(s) 336
SinI GGWCC 3 cut(s) 321, 2215, 2675
SlaI CTCGAG 1 cut(s) 116
SmlI CTYRAG 5 cut(s) 116, 138, 761, 974, 1367
SmoI CTYRAG 5 cut(s) 116, 138, 761, 974, 1367
SpeI ACTAGT 1 cut(s) 1793
SseBI AGGCCT 1 cut(s) 2033
SsiI CCGC 6 cut(s) 58, 81, 208, 1330, 1775, 3400
SspI AATATT 2 cut(s) 2023, 2572
StuI AGGCCT 1 cut(s) 2033
StyD4I CCNGG 7 cut(s) 261, 862, 1075, 2646, 2980, 3338, 3352
StyI CCWWGG 5 cut(s) 183, 447, 1622, 2657, 3174
TaaI ACNGT 9 cut(s) 1202, 1211, 1693, 1713, 2047, 2733, 2834, 3007, 3259
TaiI ACGT 1 cut(s) 2280
TaqII GACCGA 1 cut(s) 2203
TatI WGTACW 2 cut(s) 1807, 2066
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 9 cut(s) 587, 658, 670, 1293, 1421, 1441, 1682, 2266, 3155
TseFI GTSAC 2 cut(s) 378, 2371
TseI GCWGC 5 cut(s) 1157, 1943, 2318, 2549, 3240
Tsp45I GTSAC 2 cut(s) 378, 2371
Van91I CCANNNNNTGG 1 cut(s) 1508
Vha464I CTTAAG 1 cut(s) 1367
VpaK11BI GGWCC 3 cut(s) 321, 2215, 2675
VspI ATTAAT 1 cut(s) 2241
XceI RCATGY 1 cut(s) 2300
XhoI CTCGAG 1 cut(s) 116
XmiI GTMKAC 3 cut(s) 2664, 2735, 3311
XmnI GAANNNNTTC 2 cut(s) 173, 1376
Zsp2I ATGCAT 1 cut(s) 3326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.