Rmu_sc0004143.1_g000003

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004143.1
OG0014656
Physical Location & Seq
Reverse (-)
14785 .. 28188
13404 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1077 bp
atgttgaaggccaagagtttgagcaaagtagcggtgccgccttcactcatacacaatccttctcctgctaacatccaatccactcgcctcgctctccatgttagcgaggatggctcttcatgttggctctacatcgcctccggctgccaaatctacaagctacaggttgcgttggatgattctctggtcagtgaagggaaagatagccttctgatccctgtacacaggcaggttatgaactcatttatggttaatcggtgcccacatcgttcagaaattcagagtatggtgcttgctgaaactgagagcacttgcaattcaatgttgggaactgtggattcttatggtcaccttattgtatctaaactggacactaatggtgaagagaggcttacttattcagtattgcctcgtgattgcggcattggagagagtgggtgggcaggtctttgcttcaatccaatacaattgtccacggcagctgttgcacgtagcttctgcaaaaccattgatgtttatgaccaggatatccacgttaggacattatgtacactcttgtatccaacatcactgagcttcatgcaaagttcgcgttatggaaatgaaagttctatattagctgtcactgaaggttgccagctgagtatatgggacttgagaatgaaagaaaatggcggctgtctacaacgaatatgtggttcccttggtgatgtcttctacactgtatgcagttcctcaactggaaatattgcagtcggtggagctgatcgtacagtgacaatctatgatcctcgcagatgggcatcactatcaagatgggtgaactgctcgaaatatgagataactggacttgctttctcttcagttgactccaaccatatctatatacaaggggttgattacgaggtcttctgtggagactggaaaagaaacagcaaggtattttcctttagaggagattcaaattggcttggctttagcaagtgccagaacagtgatgttgtcggtggatggtgtgactcgggtagcatctttgtggctaatgttgtagctgaagacggtgaaacaaccatgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

358

Amino Acids

39.26

Weight (kDa)

5.52

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 198 - 302 7.5e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13700 FvH4_2g13700 FvH4_2g13700
malus_domestica MD05G1026600.v1.1
prunus_persica Prupe.8G031100_v2.0.a1
pyrus_communis pycom05g01840
rosa_chinensis RchiOBHm_Chr6g0275381
rosa_laevigata RLG00000013470
rosa_multiflora Rmu_sc0004143.1_g000003
rosa_roxburghii Rroxscaffold_7G00193370
rosa_rugosa Rorug06G0091200 Rorug06G0091200 Rorug06G0091200
rosa_samantha Rh6AG205100 Rh6BG207900 Rh6CG210300
rosa_wichuraiana Rw6G017800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 220, 434
AccB1I GGYRCC 2 cut(s) 34, 258
AccI GTMKAC 1 cut(s) 682
AccII CGCG 1 cut(s) 592
AciI CCGC 4 cut(s) 32, 38, 420, 675
AclWI GGATC 2 cut(s) 208, 782
AcsI RAATTY 1 cut(s) 276
AcuI CTGAAG 3 cut(s) 648, 846, 1074
AfaI GTAC 3 cut(s) 222, 550, 772
AgsI TTSAA 4 cut(s) 7, 321, 457, 963
AjnI CCWGG 1 cut(s) 522
AluBI AGCT 8 cut(s) 160, 482, 495, 576, 620, 640, 764, 1052
AluI AGCT 8 cut(s) 160, 482, 495, 576, 620, 640, 764, 1052
Alw21I GWGCWC 1 cut(s) 311
Alw26I GTCTC 1 cut(s) 912
AlwI GGATC 2 cut(s) 208, 782
Ama87I CYCGRG 1 cut(s) 1021
AoxI GGCC 1 cut(s) 9
ApeKI GCWGC 2 cut(s) 144, 479
ApoI RAATTY 1 cut(s) 276
AsuHPI GGTGA 5 cut(s) 341, 392, 719, 832, 1073
AvaI CYCGRG 1 cut(s) 1021
BaeGI GKGCMC 1 cut(s) 263
BaeI ACNNNNGTAYC 2 cut(s) 542, 575
BanI GGYRCC 2 cut(s) 34, 258
BauI CACGAG 1 cut(s) 411
BbsI GAAGAC 3 cut(s) 706, 901, 1062
Bbv12I GWGCWC 1 cut(s) 311
BbvI GCAGC 2 cut(s) 131, 491
BccI CCATC 4 cut(s) 104, 792, 810, 1005
BceAI ACGGC 1 cut(s) 492
BciT130I CCWGG 1 cut(s) 524
BciVI GTATCC 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 912
BfmI CTRYAG 1 cut(s) 161
BfuAI ACCTGC 2 cut(s) 220, 434
BfuI GTATCC 1 cut(s) 570
BisI GCNGC 5 cut(s) 38, 145, 421, 480, 676
BlsI GCNGC 5 cut(s) 39, 146, 422, 481, 677
Bme1390I CCNGG 1 cut(s) 524
BmeT110I CYCGRG 1 cut(s) 1021
BmiI GGNNCC 3 cut(s) 36, 260, 700
BmrFI CCNGG 1 cut(s) 524
BmsI GCATC 2 cut(s) 812, 1038
BpiI GAAGAC 3 cut(s) 706, 901, 1062
BplI GAGNNNNNCTC 2 cut(s) 98, 130
BpuEI CTTGAG 1 cut(s) 676
BsaAI YACGTR 1 cut(s) 491
BsaBI GATNNNNATC 1 cut(s) 802
BsaJI CCNNGG 2 cut(s) 474, 703
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bse1I ACTGG 4 cut(s) 372, 745, 850, 926
Bse8I GATNNNNATC 1 cut(s) 802
BseBI CCWGG 1 cut(s) 524
BseDI CCNNGG 2 cut(s) 474, 703
BseGI GGATG 4 cut(s) 72, 115, 181, 1016
BseJI GATNNNNATC 1 cut(s) 802
BseMII CTCAG 3 cut(s) 294, 563, 632
BseNI ACTGG 4 cut(s) 372, 745, 850, 926
BseRI GAGGAG 1 cut(s) 969
BseSI GKGCMC 1 cut(s) 263
BseXI GCAGC 2 cut(s) 131, 491
Bsh1236I CGCG 1 cut(s) 592
BshFI GGCC 1 cut(s) 11
BshNI GGYRCC 2 cut(s) 34, 258
BsiHKAI GWGCWC 1 cut(s) 311
BsiHKCI CYCGRG 1 cut(s) 1021
BsiSI CCGG 1 cut(s) 141
BslFI GGGAC 1 cut(s) 665
BsmAI GTCTC 1 cut(s) 912
BsmFI GGGAC 1 cut(s) 665
BsnI GGCC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 1021
Bsp1286I GDGCHC 2 cut(s) 263, 311
Bsp1407I TGTACA 2 cut(s) 220, 548
Bsp143I GATC 3 cut(s) 213, 766, 787
BspACI CCGC 4 cut(s) 32, 38, 420, 675
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 3 cut(s) 295, 564, 633
BspFNI CGCG 1 cut(s) 592
BspLI GGNNCC 3 cut(s) 36, 260, 700
BspMI ACCTGC 2 cut(s) 220, 434
BspPI GGATC 2 cut(s) 208, 782
BspQI GCTCTTC 1 cut(s) 121
BspT107I GGYRCC 2 cut(s) 34, 258
BsrGI TGTACA 2 cut(s) 220, 548
BsrI ACTGG 4 cut(s) 372, 745, 850, 926
BssECI CCNNGG 2 cut(s) 474, 703
BssMI GATC 3 cut(s) 213, 766, 787
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 1 cut(s) 703
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 1 cut(s) 524
Bst4CI ACNGT 5 cut(s) 334, 724, 775, 995, 1061
Bst6I CTCTTC 3 cut(s) 121, 378, 865
BstAPI GCANNNNNTGC 1 cut(s) 485
BstAUI TGTACA 2 cut(s) 220, 548
BstBAI YACGTR 1 cut(s) 491
BstC8I GCNNGC 2 cut(s) 294, 638
BstDEI CTNAG 3 cut(s) 303, 572, 641
BstDSI CCRYGG 1 cut(s) 474
BstEII GGTNACC 1 cut(s) 347
BstF5I GGATG 4 cut(s) 72, 115, 181, 1016
BstFNI CGCG 1 cut(s) 592
BstKTI GATC 3 cut(s) 216, 769, 790
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 3 cut(s) 213, 766, 787
BstMWI GCNNNNNNNGC 3 cut(s) 111, 485, 589
BstNI CCWGG 1 cut(s) 524
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 1 cut(s) 522
BstSFI CTRYAG 1 cut(s) 161
BstSLI GKGCMC 1 cut(s) 263
BstUI CGCG 1 cut(s) 592
BstV1I GCAGC 2 cut(s) 131, 491
BstV2I GAAGAC 3 cut(s) 706, 901, 1062
BsuI GTATCC 1 cut(s) 570
BsuRI GGCC 1 cut(s) 11
BtgI CCRYGG 1 cut(s) 474
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 4 cut(s) 72, 115, 181, 1016
BtsIMutI CAGTG 6 cut(s) 196, 569, 624, 720, 780, 1000
BveI ACCTGC 2 cut(s) 220, 434
Cac8I GCNNGC 2 cut(s) 294, 638
Csp6I GTAC 3 cut(s) 221, 549, 771
CviAII CATG 4 cut(s) 98, 120, 580, 1072
CviQI GTAC 3 cut(s) 221, 549, 771
DdeI CTNAG 3 cut(s) 303, 572, 641
DpnI GATC 3 cut(s) 215, 768, 789
DpnII GATC 3 cut(s) 213, 766, 787
Eam1104I CTCTTC 3 cut(s) 121, 378, 865
EarI CTCTTC 3 cut(s) 121, 378, 865
Eco130I CCWWGG 1 cut(s) 703
Eco32I GATATC 1 cut(s) 529
Eco57I CTGAAG 3 cut(s) 648, 846, 1074
Eco88I CYCGRG 1 cut(s) 1021
Eco91I GGTNACC 1 cut(s) 347
EcoO65I GGTNACC 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 522
EcoRV GATATC 1 cut(s) 529
EcoT14I CCWWGG 1 cut(s) 703
ErhI CCWWGG 1 cut(s) 703
FaeI CATG 4 cut(s) 101, 123, 583, 1075
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 375, 407
FaqI GGGAC 1 cut(s) 665
FatI CATG 4 cut(s) 97, 119, 579, 1071
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 5 cut(s) 38, 145, 421, 480, 676
FokI GGATG 4 cut(s) 59, 122, 188, 1023
Fsp4HI GCNGC 5 cut(s) 38, 145, 421, 480, 676
GluI GCNGC 5 cut(s) 38, 145, 421, 480, 676
HaeIII GGCC 1 cut(s) 11
HapII CCGG 1 cut(s) 141
Hin1II CATG 4 cut(s) 101, 123, 583, 1075
HincII GTYRAC 1 cut(s) 868
HindII GTYRAC 1 cut(s) 868
HinfI GANTC 5 cut(s) 179, 338, 869, 959, 1019
HpaII CCGG 1 cut(s) 141
HphI GGTGA 5 cut(s) 341, 392, 719, 832, 1073
Hpy166II GTNNAC 6 cut(s) 223, 474, 551, 683, 823, 868
Hpy188I TCNGA 3 cut(s) 213, 274, 282
Hpy188III TCNNGA 2 cut(s) 413, 813
Hpy8I GTNNAC 6 cut(s) 223, 474, 551, 683, 823, 868
HpyAV CCTTC 5 cut(s) 51, 69, 188, 218, 623
HpyCH4III ACNGT 5 cut(s) 334, 724, 775, 995, 1061
HpyCH4IV ACGT 2 cut(s) 490, 534
HpyCH4V TGCA 6 cut(s) 315, 488, 501, 583, 729, 752
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 485, 589
HpyF3I CTNAG 3 cut(s) 303, 572, 641
HpySE526I ACGT 2 cut(s) 490, 534
Hsp92II CATG 4 cut(s) 101, 123, 583, 1075
Kzo9I GATC 3 cut(s) 213, 766, 787
LguI GCTCTTC 1 cut(s) 121
LmnI GCTCC 1 cut(s) 761
Lsp1109I GCAGC 2 cut(s) 131, 491
LweI GCATC 2 cut(s) 812, 1038
MaeII ACGT 2 cut(s) 490, 534
MaeIII GTNAC 4 cut(s) 347, 622, 775, 1016
MalI GATC 3 cut(s) 215, 768, 789
MboI GATC 3 cut(s) 213, 766, 787
MboII GAAGA 6 cut(s) 108, 395, 706, 852, 901, 1067
MfeI CAATTG 1 cut(s) 467
MhlI GDGCHC 2 cut(s) 263, 311
MluCI AATT 4 cut(s) 276, 316, 467, 964
MlyI GAGTC 2 cut(s) 863, 1013
MmeI TCCRAC 3 cut(s) 153, 587, 897
MnlI CCTC 9 cut(s) 98, 100, 148, 381, 420, 745, 801, 898, 947
MseI TTAA 1 cut(s) 252
MslI CAYNNNNRTG 1 cut(s) 1034
MspA1I CMGCKG 2 cut(s) 482, 640
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 1 cut(s) 524
MunI CAATTG 1 cut(s) 467
MvaI CCWGG 1 cut(s) 524
MvnI CGCG 1 cut(s) 592
MwoI GCNNNNNNNGC 3 cut(s) 111, 485, 589
NdeII GATC 3 cut(s) 213, 766, 787
NlaIII CATG 4 cut(s) 101, 123, 583, 1075
NlaIV GGNNCC 3 cut(s) 36, 260, 700
NmuCI GTSAC 4 cut(s) 347, 622, 775, 1016
PciSI GCTCTTC 1 cut(s) 121
PfeI GAWTC 3 cut(s) 179, 338, 959
PkrI GCNGC 5 cut(s) 39, 146, 422, 481, 677
PleI GAGTC 2 cut(s) 863, 1013
PpsI GAGTC 2 cut(s) 863, 1013
Ppu21I YACGTR 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 522
PspEI GGTNACC 1 cut(s) 347
PspGI CCWGG 1 cut(s) 522
PspN4I GGNNCC 3 cut(s) 36, 260, 700
PvuII CAGCTG 2 cut(s) 482, 640
RsaI GTAC 3 cut(s) 222, 550, 772
RsaNI GTAC 3 cut(s) 221, 549, 771
RseI CAYNNNNRTG 1 cut(s) 1034
SapI GCTCTTC 1 cut(s) 121
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 5 cut(s) 38, 145, 421, 480, 676
Sau3AI GATC 3 cut(s) 213, 766, 787
SchI GAGTC 2 cut(s) 863, 1013
ScrFI CCNGG 1 cut(s) 524
SduI GDGCHC 2 cut(s) 263, 311
SfaNI GCATC 2 cut(s) 812, 1038
SfcI CTRYAG 1 cut(s) 161
SmiMI CAYNNNNRTG 1 cut(s) 1034
SmlI CTYRAG 1 cut(s) 655
SmoI CTYRAG 1 cut(s) 655
Sse9I AATT 4 cut(s) 276, 316, 467, 964
SsiI CCGC 4 cut(s) 32, 38, 420, 675
SspI AATATT 1 cut(s) 748
StyD4I CCNGG 1 cut(s) 522
StyI CCWWGG 1 cut(s) 703
TaaI ACNGT 5 cut(s) 334, 724, 775, 995, 1061
TaiI ACGT 2 cut(s) 493, 537
TaqI TCGA 1 cut(s) 830
TasI AATT 4 cut(s) 276, 316, 467, 964
TatI WGTACW 2 cut(s) 220, 548
TauI GCSGC 3 cut(s) 40, 423, 678
TfiI GAWTC 3 cut(s) 179, 338, 959
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 6 cut(s) 196, 576, 631, 727, 780, 1000
TseFI GTSAC 4 cut(s) 347, 622, 775, 1016
TseI GCWGC 2 cut(s) 144, 479
Tsp45I GTSAC 4 cut(s) 347, 622, 775, 1016
TspDTI ATGAA 5 cut(s) 108, 251, 568, 618, 677
TspRI CASTG 6 cut(s) 196, 576, 631, 727, 780, 1000
XapI RAATTY 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.